##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431404_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3183772
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.594862634635895	34.0	31.0	34.0	31.0	34.0
2	32.74095883750469	34.0	31.0	34.0	31.0	34.0
3	32.82296156885606	34.0	31.0	34.0	31.0	34.0
4	36.293245244948444	37.0	37.0	37.0	35.0	37.0
5	36.210977733330154	37.0	37.0	37.0	35.0	37.0
6	36.262698773655906	37.0	37.0	37.0	35.0	37.0
7	36.21461775529152	37.0	37.0	37.0	35.0	37.0
8	36.23177727550842	37.0	37.0	37.0	35.0	37.0
9	38.02338389809321	39.0	39.0	39.0	35.0	39.0
10-11	37.99925528586846	39.0	39.0	39.0	35.0	39.0
12-13	37.98413626980826	39.0	39.0	39.0	35.0	39.0
14-15	39.42958698047473	41.0	39.0	41.0	36.0	41.0
16-17	39.45819047343842	41.0	39.0	41.0	36.0	41.0
18-19	39.53800240720755	41.0	39.5	41.0	36.5	41.0
20-21	39.53433270347249	41.0	40.0	41.0	36.0	41.0
22-23	39.46936998001113	41.0	39.0	41.0	36.0	41.0
24-25	39.41903723005291	41.0	39.0	41.0	36.0	41.0
26-27	39.29827245795239	41.0	39.0	41.0	35.5	41.0
28-29	39.21424681164355	41.0	39.0	41.0	35.0	41.0
30-31	39.02169235108545	41.0	39.0	41.0	35.0	41.0
32-33	38.78573025957889	41.0	38.0	41.0	35.0	41.0
34-35	38.64545953667536	41.0	38.0	41.0	35.0	41.0
36-37	38.4999484887737	41.0	38.0	41.0	35.0	41.0
38-39	38.352762854877795	40.0	38.0	41.0	35.0	41.0
40-41	38.20764316665892	40.0	38.0	41.0	34.0	41.0
42-43	38.025367865538115	40.0	38.0	41.0	34.0	41.0
44-45	37.76830061951672	40.0	38.0	41.0	33.0	41.0
46-47	37.385388149653934	40.0	38.0	41.0	33.0	41.0
48-49	37.040894731155376	40.0	37.0	41.0	31.5	41.0
50-51	36.869334236245564	40.0	37.0	41.0	31.0	41.0
52-53	36.809926244718525	40.0	37.0	41.0	31.0	41.0
54-55	36.66996521735852	40.0	36.0	41.0	31.0	41.0
56-57	36.52056428663862	40.0	36.0	41.0	31.0	41.0
58-59	36.33828553049653	40.0	35.5	41.0	31.0	41.0
60-61	36.18161429273202	39.5	35.0	41.0	30.5	41.0
62-63	36.020607631451	39.0	35.0	41.0	30.0	41.0
64-65	35.78781976221916	39.0	35.0	41.0	30.0	41.0
66-67	35.52015847868503	38.5	35.0	41.0	29.5	41.0
68-69	35.22420716684486	37.5	35.0	40.5	29.0	41.0
70-71	34.928840695878975	37.0	35.0	40.0	29.0	41.0
72-73	34.608784956963	37.0	35.0	39.5	29.0	41.0
74-75	34.27252752395586	36.0	35.0	39.0	28.5	41.0
76-77	33.943495953855994	36.0	35.0	39.0	28.0	41.0
78-79	33.623446025657614	35.0	34.0	37.5	28.0	39.5
80-81	33.36020654117192	35.0	34.0	37.0	28.0	39.0
82-83	33.1610456087936	35.0	34.0	37.0	29.0	39.0
84-85	32.90039236477989	35.0	34.0	36.0	28.5	38.5
86-87	32.65812438830419	35.0	34.0	36.0	28.0	37.0
88-89	32.42709261215941	35.0	34.0	36.0	27.0	37.0
90-91	32.2432733876672	35.0	34.0	35.5	27.0	36.5
92-93	32.084867415128976	35.0	34.0	35.0	27.0	36.0
94-95	31.94364577614226	35.0	34.0	35.0	26.5	36.0
96-97	31.79755978129087	35.0	34.0	35.0	26.0	36.0
98-99	31.685517367449677	35.0	34.0	35.0	25.5	36.0
100	31.58069076554477	35.0	34.0	35.0	25.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.04999795311083233
1101	2	-0.021299523535148523
1101	3	-0.028502577225644643
1101	4	0.01589258553414652
1101	5	-0.0069436937780480434
1101	6	-0.023943481547732404
1101	7	0.03923993497772926
1101	8	0.03825367958822312
1101	9	-0.03433802805648156
1101	10-11	0.051954128983695114
1101	12-13	0.052226181209675815
1101	14-15	0.06366739141576971
1101	16-17	0.015287696008677187
1101	18-19	-0.0198628788127877
1101	20-21	0.026306578926053703
1101	22-23	0.022134302688137097
1101	24-25	0.027456281809733696
1101	26-27	0.07124352683300117
1101	28-29	3.638086046677813E-4
1101	30-31	-0.00510197742284646
1101	32-33	-0.027118632465644055
1101	34-35	0.04630746051981305
1101	36-37	0.055796727908528965
1101	38-39	-0.029269171921569637
1101	40-41	-0.03434624410515852
1101	42-43	-0.014350316976894817
1101	44-45	-0.03399398676040022
1101	46-47	-0.06973172272298456
1101	48-49	-0.05258018955758814
1101	50-51	-0.17481075789942935
1101	52-53	-0.10755102210242029
1101	54-55	-0.0548492382016974
1101	56-57	0.029355403649340417
1101	58-59	0.04892418801581755
1101	60-61	0.03539120966919285
1101	62-63	-0.03654707212454156
1101	64-65	-0.012747489480943841
1101	66-67	7.717282598562747E-4
1101	68-69	-0.014691680926361528
1101	70-71	0.03382299847780246
1101	72-73	0.018540876157423725
1101	74-75	0.01926130547033722
1101	76-77	0.02102976528312439
1101	78-79	0.022799862185173936
1101	80-81	0.06733561058896953
1101	82-83	-0.018441381069770557
1101	84-85	0.020745261267457238
1101	86-87	0.025111306370995123
1101	88-89	0.019228470600523906
1101	90-91	-0.010686425013730627
1101	92-93	-0.012352261183572466
1101	94-95	-0.028689468508119376
1101	96-97	0.01070442398314242
1101	98-99	-0.06322891236998274
1101	100	0.001655830768758193
1102	1	-0.017796293395022644
1102	2	0.010011486816097204
1102	3	-0.016707132479652387
1102	4	0.02461649606006233
1102	5	0.02873558060249337
1102	6	0.032175082437888136
1102	7	0.002270100390333596
1102	8	0.028678735039264325
1102	9	0.05959184861409028
1102	10-11	0.048876425161260784
1102	12-13	0.008302671290408625
1102	14-15	-0.052633533027240276
1102	16-17	0.001130569224976341
1102	18-19	0.015696946499318187
1102	20-21	0.02749415780012754
1102	22-23	0.028076998215247784
1102	24-25	0.07604245712246183
1102	26-27	0.0754382765941557
1102	28-29	0.09149127096603848
1102	30-31	0.07760198302118937
1102	32-33	0.08799826178434955
1102	34-35	0.11031330447850962
1102	36-37	0.056453373068954704
1102	38-39	0.05777876844017982
1102	40-41	0.1208168201090345
1102	42-43	0.1014847792932585
1102	44-45	0.10112649791985007
1102	46-47	0.07326198637005632
1102	48-49	0.026544172230671848
1102	50-51	0.02549011458903294
1102	52-53	0.048101203442463714
1102	54-55	0.013178212353935237
1102	56-57	0.05958747579379775
1102	58-59	0.010810518426794147
1102	60-61	0.00891099797296846
1102	62-63	0.019496644842867283
1102	64-65	0.016956733751037234
1102	66-67	0.013193573198265085
1102	68-69	0.04638508632260141
1102	70-71	-0.0012465058039268229
1102	72-73	0.06413936353791883
1102	74-75	0.10227049887368622
1102	76-77	0.04355725654420439
1102	78-79	0.038670422071945154
1102	80-81	0.05177884224524121
1102	82-83	0.07253200359031808
1102	84-85	0.07300682643063539
1102	86-87	0.04101243594169546
1102	88-89	0.08801466627757293
1102	90-91	0.01849025082770339
1102	92-93	-0.03283174598002603
1102	94-95	-0.06730684206200266
1102	96-97	-0.04658404505322267
1102	98-99	0.03410125018976373
1102	100	0.049137693645402436
1103	1	-0.07191103166434942
1103	2	-0.07556155442723878
1103	3	-0.07191478332960344
1103	4	-0.03273319324136281
1103	5	-0.03727999739709276
1103	6	-0.007346349147340447
1103	7	-0.039469765445893756
1103	8	-0.019255703138682634
1103	9	0.017307420216560843
1103	10-11	0.03784843284425676
1103	12-13	-0.003418472032308273
1103	14-15	0.0011215583902171034
1103	16-17	-0.021693853689974674
1103	18-19	-0.06746357636387756
1103	20-21	-0.07898830753943287
1103	22-23	-0.01309768188889393
1103	24-25	-0.06812018094366579
1103	26-27	-0.03378289040017535
1103	28-29	-0.025956993858578414
1103	30-31	-0.019599408823445685
1103	32-33	-0.06896104221019073
1103	34-35	-0.04921909225956256
1103	36-37	-0.08991446623937094
1103	38-39	-0.07345175588488928
1103	40-41	-0.024673760783109344
1103	42-43	0.014194148933228234
1103	44-45	-0.018211385135920466
1103	46-47	0.13266842703479398
1103	48-49	0.1342118809871593
1103	50-51	0.04227527403524789
1103	52-53	0.14376788958528408
1103	54-55	0.1717987554800331
1103	56-57	0.13469660192792787
1103	58-59	0.07766009930067952
1103	60-61	0.026815027943776215
1103	62-63	9.329505378374847E-4
1103	64-65	0.006472957581500793
1103	66-67	0.05214485378632361
1103	68-69	0.0456559775599743
1103	70-71	0.03395763933793461
1103	72-73	0.013445369591842393
1103	74-75	0.04830663538013624
1103	76-77	0.059563677671199855
1103	78-79	0.042146500233073425
1103	80-81	0.06847931505394911
1103	82-83	0.06166345401815221
1103	84-85	0.0839534576830232
1103	86-87	0.08300562267422151
1103	88-89	0.025571146167905567
1103	90-91	0.04000472134222832
1103	92-93	0.05268178136507373
1103	94-95	0.002152666894065902
1103	96-97	0.012231367406727145
1103	98-99	-0.001186357594018972
1103	100	0.07591012584701673
1104	1	-0.1098733491118864
1104	2	-0.04218152220072113
1104	3	-0.05270970780308204
1104	4	-0.028149267437278525
1104	5	-0.045478993560770675
1104	6	-0.03727737447803747
1104	7	-0.057218914644074914
1104	8	-0.06938116672872496
1104	9	-0.06403642512238861
1104	10-11	-0.08026488933736431
1104	12-13	-0.05809477633458471
1104	14-15	-0.07083195404799625
1104	16-17	-0.03893658679563572
1104	18-19	-0.017997718310226674
1104	20-21	-0.04890226989479629
1104	22-23	-0.04323412189108211
1104	24-25	0.01849832124227646
1104	26-27	-0.009623094510580188
1104	28-29	-0.02278551893156333
1104	30-31	0.05014180013497338
1104	32-33	0.09375369530243916
1104	34-35	0.05722227890694853
1104	36-37	0.10375517993882255
1104	38-39	0.0487839413980069
1104	40-41	-0.049454927509273716
1104	42-43	-0.0831384999731597
1104	44-45	-0.07638245037288272
1104	46-47	-0.08015892283385284
1104	48-49	0.06911844385717814
1104	50-51	-0.005495998425587345
1104	52-53	0.05355464204108529
1104	54-55	0.1568364784090619
1104	56-57	0.1278923520157207
1104	58-59	0.11947434628808651
1104	60-61	0.09567373992747719
1104	62-63	0.10978404201319591
1104	64-65	0.15111753847586584
1104	66-67	0.09149838567456214
1104	68-69	0.06377266293688422
1104	70-71	0.054112111170901755
1104	72-73	0.0809102011873577
1104	74-75	0.0852406946046429
1104	76-77	0.10573458413618653
1104	78-79	0.10903731004219708
1104	80-81	0.05488102330195943
1104	82-83	0.05526442812346488
1104	84-85	0.07001269421581924
1104	86-87	0.0182777165658905
1104	88-89	0.06858181114270678
1104	90-91	0.038465899997255804
1104	92-93	0.035519505187316724
1104	94-95	0.03919808069423425
1104	96-97	0.014025299418594983
1104	98-99	0.006585767970012313
1104	100	0.0882077086574995
1105	1	-0.14193284280708696
1105	2	-0.1317156294089017
1105	3	-0.09461173894538888
1105	4	-0.0117372212906659
1105	5	-0.11618218573869399
1105	6	-0.029717644260692566
1105	7	0.014593095142004131
1105	8	-0.049673400002539836
1105	9	0.019014339811221248
1105	10-11	-0.014811282743160348
1105	12-13	-0.014176075629578122
1105	14-15	-0.02293615243893754
1105	16-17	-0.05608359368493865
1105	18-19	-0.12085925659308572
1105	20-21	-0.03638913163677415
1105	22-23	-0.11784980132160428
1105	24-25	-0.09898184908939811
1105	26-27	-0.05554758788640157
1105	28-29	-0.03149770833152843
1105	30-31	-0.007933811907953725
1105	32-33	-0.14158050222638252
1105	34-35	-0.13517321024273343
1105	36-37	-0.1258377830918107
1105	38-39	-0.1055623707996105
1105	40-41	-0.1654370125759499
1105	42-43	-0.20778893185500635
1105	44-45	-0.13635271871272892
1105	46-47	-0.14454317684658236
1105	48-49	-0.04646036983350399
1105	50-51	-0.1388460230605233
1105	52-53	-0.10290607540495955
1105	54-55	-0.05757906621053621
1105	56-57	-0.06306421590723232
1105	58-59	-0.06977778810652069
1105	60-61	-0.13236655236833172
1105	62-63	-0.14796506226247175
1105	64-65	-0.08860681383537639
1105	66-67	-0.11497378935987257
1105	68-69	-0.051918515524107534
1105	70-71	-0.015898935463049213
1105	72-73	-0.02253087218989691
1105	74-75	-0.024613925796302283
1105	76-77	-0.042592786635395896
1105	78-79	-0.016337563042121417
1105	80-81	-0.06922489395057596
1105	82-83	-0.14461941024458014
1105	84-85	-0.19644261381909445
1105	86-87	-0.16788788199148286
1105	88-89	-0.10412268447549167
1105	90-91	-0.1523075475218505
1105	92-93	-0.17930737355566606
1105	94-95	-0.20039724532441028
1105	96-97	-0.24366220636045455
1105	98-99	-0.16257737245445725
1105	100	-0.15437507744384504
1106	1	-0.10412839381448435
1106	2	-0.06557371928004585
1106	3	-0.0713317991988589
1106	4	-0.0344065473569799
1106	5	-0.025130078126828437
1106	6	-0.08579724208440354
1106	7	-0.049408235716100535
1106	8	-0.024712383199158694
1106	9	-0.10188894971904006
1106	10-11	0.0012087044521109647
1106	12-13	-0.07491237374178894
1106	14-15	-0.06316739772076829
1106	16-17	-0.07075684717257502
1106	18-19	-0.06516766645220429
1106	20-21	-0.02687829582835377
1106	22-23	-0.07091944029594544
1106	24-25	-0.014637435696386092
1106	26-27	-0.05811146603470263
1106	28-29	-0.014953478583031199
1106	30-31	-0.08521831410013903
1106	32-33	-0.07842839696002102
1106	34-35	-0.029315718910254418
1106	36-37	-0.04525243573524307
1106	38-39	0.013857407211176564
1106	40-41	0.002685547988086512
1106	42-43	-0.0019118511947482375
1106	44-45	-0.013956079563783419
1106	46-47	-0.039293302157467735
1106	48-49	0.02882595577326441
1106	50-51	-0.07626441895953917
1106	52-53	-0.11074124185343948
1106	54-55	0.0016058309824131811
1106	56-57	-5.687223213257653E-4
1106	58-59	0.01981567470460277
1106	60-61	0.005097428895403766
1106	62-63	0.0037520661071326344
1106	64-65	0.023641011575506354
1106	66-67	-0.0019545092915578266
1106	68-69	0.01534343939525229
1106	70-71	0.08296143041868476
1106	72-73	0.046400280227238966
1106	74-75	-0.02511367083099003
1106	76-77	0.0038140919550784247
1106	78-79	0.05662970757814634
1106	80-81	-0.0025161963759217087
1106	82-83	-0.032642360597144204
1106	84-85	0.03184761141039161
1106	86-87	-0.0032801941141968882
1106	88-89	-0.026722144835119366
1106	90-91	-0.055477652383387976
1106	92-93	-0.019229772019095037
1106	94-95	-0.007360773557799405
1106	96-97	-0.019839672639442085
1106	98-99	0.05394692048374239
1106	100	0.11054738617256277
1107	1	-0.3015014269017797
1107	2	-0.21205452868830577
1107	3	-0.15775444413429796
1107	4	-0.21597650815139247
1107	5	-0.2860668445431358
1107	6	-0.237425073284399
1107	7	-0.19645571608373302
1107	8	-0.20664798450075494
1107	9	-0.16933048357378766
1107	10-11	-0.1924398993876011
1107	12-13	-0.16259967683081555
1107	14-15	-0.2510667756840519
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2216	42-43	-0.22867167318165116
2216	44-45	-0.2435941854131869
2216	46-47	-0.27443473491570103
2216	48-49	-0.254566170743189
2216	50-51	-0.24571401201625065
2216	52-53	-0.26513947231843105
2216	54-55	-0.2648738868745255
2216	56-57	-0.2862558500057517
2216	58-59	-0.3531793700515351
2216	60-61	-0.4150611942965483
2216	62-63	-0.3210546569057371
2216	64-65	-0.32857293406852506
2216	66-67	-0.42117618762052444
2216	68-69	-0.38283980508940374
2216	70-71	-0.35940375732750596
2216	72-73	-0.2941212204578889
2216	74-75	-0.32736699031458016
2216	76-77	-0.361117282773904
2216	78-79	-0.34956107613385257
2216	80-81	-0.36844859378118144
2216	82-83	-0.28979421116761017
2216	84-85	-0.2950620800126913
2216	86-87	-0.34316232144140457
2216	88-89	-0.28696538365250746
2216	90-91	-0.2540815668093117
2216	92-93	-0.16706930817038312
2216	94-95	-0.22752906962980646
2216	96-97	-0.23364499970721297
2216	98-99	-0.238105243231157
2216	100	-0.21030336971685415
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	42.0
3	283.0
4	72.0
5	127.0
6	317.0
7	656.0
8	1135.0
9	2049.0
10	2882.0
11	4081.0
12	6377.0
13	9487.0
14	10871.0
15	14240.0
16	18592.0
17	28350.0
18	32905.0
19	16977.0
20	10218.0
21	8951.0
22	8739.0
23	8640.0
24	9394.0
25	10863.0
26	13477.0
27	17415.0
28	21572.0
29	27107.0
30	34367.0
31	43264.0
32	54824.0
33	71059.0
34	100221.0
35	168058.0
36	266178.0
37	545995.0
38	983654.0
39	623935.0
40	6398.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.550895969081466	21.210506873996415	14.788170092147487	22.45042706477463
2	14.423544156286761	22.156277337069106	40.60924069499173	22.810937811652405
3	16.305676587062276	28.641875728701805	32.22705720057894	22.825390483656978
4	10.872072497653726	18.760325802224532	40.08031982189679	30.28728187822495
5	10.888555470891824	39.47469383586932	35.87638745964289	13.760363233595966
6	26.86432948087991	40.571655256720646	18.530409840905694	14.03360542149375
7	23.707916270386196	35.50530628449525	23.84102881739019	16.945748627728367
8	24.698439461117193	35.243227216019235	21.252526876924605	18.80580644593897
9	25.715488241927027	15.71539401401483	22.395524802356952	36.17359294170119
10-11	22.53677399009728	27.656754315321574	29.845745863711347	19.960725830869798
12-13	24.237209825326687	26.241640418974725	29.823476681119125	19.697673074579463
14-15	21.50485673987596	27.092098160939	28.887830046848524	22.51521505233651
16-17	18.98033213433625	30.2699596579152	29.51517885074685	21.234529357001694
18-19	18.849925803336518	27.82100834278623	33.83777746403358	19.49128838984367
20-21	20.450977645384157	26.846190619177502	33.756233172475916	18.946598562962425
22-23	19.94891589984765	26.8814215350703	33.92700068268133	19.242661882400718
24-25	19.57783402483501	27.06415959813699	33.88272368164612	19.475282695381875
26-27	19.291239408386726	27.14374064198753	33.890853890467554	19.674166059158186
28-29	19.311221797568166	27.050180792067763	34.15738835241764	19.481209057946433
30-31	19.639696855623857	27.3534293611947	33.707952867730285	19.298920915451163
32-33	19.303099247438023	27.19406154646861	33.98159922443038	19.521239981662987
34-35	19.695596954461884	27.03076052547641	33.70561567615692	19.568026843904786
36-37	19.798990523852442	27.185592499113852	33.2558613236893	19.759555653344403
38-39	19.67208326069852	27.434271382511422	33.278413561430206	19.615231795359854
40-41	19.652242358035398	27.22645707022086	33.303295559445424	19.81800501229832
42-43	19.63587359211146	27.398416399241526	33.02348451844786	19.94222549019916
44-45	19.91542718834035	27.478044472810577	32.669554337466124	19.936974001382954
46-47	20.07280682399076	27.731107877695216	32.03808067014944	20.158004628164587
48-49	20.26097693047986	27.849391036030262	31.43316347171025	20.45646856177963
50-51	20.275837374676485	27.944380229665555	31.17295273513079	20.606829660527175
52-53	20.222005531528158	28.14469228813385	31.144959740080054	20.48834244025794
54-55	20.40091791299803	28.36829845615728	30.961738757890085	20.26904487295461
56-57	20.293114422800727	27.989319363867043	30.96908617961052	20.748480033721712
58-59	20.20619670602898	27.709378482521902	31.339242077596396	20.745182733852722
60-61	20.320102370441727	27.99892201964896	31.061570361696646	20.619405248212672
62-63	20.139524934887735	27.89105678493039	31.25154692668855	20.717871353493322
64-65	20.20927696767637	27.762916597499753	31.313067859065026	20.714738575758858
66-67	20.13168075927822	27.811953820018807	31.17073347696851	20.885631943734463
68-69	20.563581257344882	28.00112885233817	30.86852058310217	20.566769307214777
70-71	20.470062738583373	27.756385400750965	30.943836490521527	20.829715370144136
72-73	20.351392141065688	27.88915011001126	30.94966179978736	20.809795949135694
74-75	20.398256459947813	27.98234159219349	30.772539636796516	20.846862311062182
76-77	20.45885497955085	27.916599005387045	30.681199628991084	20.94334638607102
78-79	20.505599934668084	27.95020325921459	30.82824694522249	20.715949860894845
80-81	20.41195131527287	27.895987436199448	30.79122104436592	20.900840204161756
82-83	20.27211474086767	27.92224404672443	30.964681212809943	20.840959999597956
84-85	20.236373316246485	27.879832963484713	30.682625909505663	21.201167810763145
86-87	20.35500020180817	27.93409840354818	30.60878693187663	21.102114462767023
88-89	20.213277533114365	27.87243238217706	30.661417283263482	21.252872801445093
90-91	20.098512976706974	27.82061868687265	30.668775714622694	21.412092621797676
92-93	20.072760395604224	27.913733465777252	30.66772935896882	21.345776779649704
94-95	19.9854102751553	27.67501191598894	30.847468274415608	21.49210953444015
96-97	20.027756410719498	27.82833804839484	30.61641720866055	21.527488332225108
98-99	19.902376527398552	27.75883443585468	30.634629524873553	21.704159511873215
100	19.898893291433257	27.790304874888694	30.52408076601131	21.78672106766674
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4071.0
1	2300.0
2	2540.5
3	4365.0
4	2547.0
5	1419.0
6	3647.0
7	9349.0
8	15834.0
9	16818.0
10	10380.0
11	4487.5
12	3530.0
13	3781.0
14	4204.5
15	4559.0
16	5017.0
17	5655.0
18	6317.5
19	7062.5
20	8224.5
21	10120.0
22	13193.0
23	17865.0
24	23107.0
25	28542.5
26	35817.5
27	44520.0
28	55669.0
29	66095.0
30	72549.5
31	78955.5
32	85828.0
33	92152.0
34	97857.0
35	102226.5
36	104031.0
37	105711.5
38	107567.0
39	107338.0
40	107312.5
41	106651.5
42	104671.0
43	103901.5
44	102935.0
45	100733.0
46	98598.0
47	96055.0
48	92431.5
49	89942.5
50	89400.5
51	84751.0
52	75997.5
53	68632.0
54	64062.0
55	61384.0
56	56084.0
57	49382.5
58	45062.0
59	41315.5
60	35820.5
61	29220.0
62	24245.0
63	19743.5
64	14876.5
65	11558.0
66	9149.0
67	6779.5
68	5004.0
69	3775.5
70	2765.5
71	2109.5
72	1604.5
73	1184.0
74	1038.5
75	812.0
76	491.5
77	375.5
78	311.0
79	182.0
80	87.0
81	50.5
82	22.0
83	11.5
84	9.0
85	4.0
86	3.5
87	3.0
88	3.0
89	2.5
90	2.0
91	2.5
92	1.0
93	0.5
94	0.5
95	0.5
96	0.5
97	0.5
98	0.5
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00565367117997143
2	0.004303071953644922
3	8.794599613288891E-4
4	0.0
5	0.03916737756346874
6	0.0
7	0.0
8	0.0
9	6.281856866634923E-5
10-11	0.0
12-13	0.0
14-15	1.570464216658731E-5
16-17	0.0
18-19	1.0993249516611113E-4
20-21	0.0
22-23	7.852321083293652E-5
24-25	1.570464216658731E-5
26-27	9.422785299952383E-5
28-29	1.2563713733269847E-4
30-31	2.669789168319842E-4
32-33	0.0014919410058257942
34-35	0.0010208017408281748
36-37	0.0013034852998267464
38-39	0.0011621435203274606
40-41	9.422785299952383E-5
42-43	4.711392649976192E-4
44-45	2.0416034816563496E-4
46-47	1.4134177949928574E-4
48-49	1.2563713733269847E-4
50-51	3.769114119980953E-4
52-53	7.067088974964287E-4
54-55	8.794599613288891E-4
56-57	0.0020887174081561117
58-59	5.025485493307939E-4
60-61	0.001272076015493572
62-63	0.0012563713733269844
64-65	0.0015233502901589686
66-67	3.6120676983150804E-4
68-69	2.355696324988096E-4
70-71	1.7275106383246035E-4
72-73	2.1986499033222227E-4
74-75	6.12481044496905E-4
76-77	6.595949709966669E-4
78-79	9.265738878286511E-4
80-81	6.910042553298414E-4
82-83	0.0013034852998267464
84-85	0.0011621435203274606
86-87	0.001743215280491191
88-89	0.0010365063829947622
90-91	9.108692456620636E-4
92-93	0.001083620309494524
94-95	7.695274661627781E-4
96-97	9.422785299952383E-5
98-99	2.826835589985715E-4
100	2.1986499033222227E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3183772.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.37782517313723
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.07187582412166	43.82532842280041
2	15.633584745925955	18.253093542541805
3	4.661763036284655	8.16430762592457
4	1.7938264875580356	4.188787563264233
5	0.8576714234139224	2.503449620602686
6	0.48791074765554926	1.7089900996038199
7	0.29899265600637237	1.2218178700274376
8	0.18886564304446032	0.8820452392689316
9	0.1363139270103496	0.7161939539706581
>10	0.7588682667650902	8.146541129686435
>50	0.07131256050857818	2.9217749406064475
>100	0.03664393052511814	3.7284040532792417
>500	0.0015625405512119832	0.6238662898895688
>1k	5.388070860315913E-4	0.7262188609757895
>5k	1.0776141720631828E-4	0.3279434270847878
>10k+	1.616421258094774E-4	2.061237360473166
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27628	0.8677757075569482	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22172	0.6964066522351475	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15731	0.4940994518451698	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5372	0.16873067543781403	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5054	0.15874252301986447	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4995	0.15688937524420718	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4461	0.14011681741029194	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4114	0.12921779574668035	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.140928433317461E-5	0.0
4	0.0	0.0	0.0	3.140928433317461E-5	0.0
5	0.0	0.0	0.0	3.140928433317461E-5	0.0
6	0.0	0.0	0.0	3.140928433317461E-5	0.0
7	0.0	0.0	0.0	3.140928433317461E-5	0.0
8	0.0	0.0	0.0	3.140928433317461E-5	0.0
9	0.0	0.0	0.0	1.8845570599904766E-4	0.0
10-11	0.0	0.0	0.0	2.0416034816563496E-4	0.0
12-13	0.0	3.140928433317461E-5	0.0	2.1986499033222227E-4	0.0
14-15	0.0	3.140928433317461E-5	0.0	2.1986499033222227E-4	0.0
16-17	0.0	3.140928433317461E-5	0.0	2.826835589985715E-4	0.0
18-19	0.0	3.140928433317461E-5	0.0	3.140928433317461E-4	0.0
20-21	0.0	3.140928433317461E-5	0.0	3.61206769831508E-4	0.0
22-23	0.0	3.140928433317461E-5	0.0	4.711392649976192E-4	0.0
24-25	0.0	3.140928433317461E-5	0.0	7.852321083293653E-4	0.0
26-27	0.0	4.7113926499761915E-5	0.0	0.0013034852998267464	0.0
28-29	0.0	6.281856866634922E-5	0.0	0.0026069705996534928	0.0
30-31	0.0	6.281856866634922E-5	0.0	0.006674472920799605	0.0
32-33	0.0	6.281856866634922E-5	0.0	0.013820085106596828	0.0
34-35	0.0	6.281856866634922E-5	0.0	0.02577131779536977	0.0
36-37	0.0	6.281856866634922E-5	0.0	0.04491527659643969	0.0
38-39	0.0	6.281856866634922E-5	0.0	0.07048243404364382	0.0
40-41	0.0	6.281856866634922E-5	0.0	0.09783992069783892	0.0
42-43	0.0	6.281856866634922E-5	0.0	0.12513458878336764	0.0
44-45	0.0	6.281856866634922E-5	0.0	0.1590880251475294	0.0
46-47	0.0	6.281856866634922E-5	0.0	0.20175753791414713	0.0
48-49	0.0	6.281856866634922E-5	0.0	0.25105440967506465	0.0
50-51	0.0	6.281856866634922E-5	0.0	0.3000057793083173	0.0
52-53	0.0	6.281856866634922E-5	0.0	0.3487372839512377	0.0
54-55	0.0	6.281856866634922E-5	0.0	0.40340514333312816	0.0
56-57	0.0	6.281856866634922E-5	0.0	0.4717360413999495	0.0
58-59	0.0	6.281856866634922E-5	0.0	0.5491128133547252	0.0
60-61	0.0	6.281856866634922E-5	0.0	0.6331326489459672	0.0
62-63	0.0	6.281856866634922E-5	0.0	0.7185816069743688	0.0
64-65	0.0	6.281856866634922E-5	0.0	0.8098098733200745	0.0
66-67	0.0	6.281856866634922E-5	0.0	0.9128637352172204	0.0
68-69	0.0	6.281856866634922E-5	0.0	1.0337580706156095	0.0
70-71	0.0	6.281856866634922E-5	0.0	1.1714720777744136	0.0
72-73	0.0	6.281856866634922E-5	0.0	1.3176351824188415	0.0
74-75	0.0	6.281856866634922E-5	0.0	1.4654472744907614	0.0
76-77	0.0	6.281856866634922E-5	0.0	1.6298591733327639	0.0
78-79	0.0	6.281856866634922E-5	0.0	1.8222567445156248	0.0
80-81	0.0	6.281856866634922E-5	0.0	2.041477844519017	0.0
82-83	0.0	6.281856866634922E-5	0.0	2.2704201180235266	0.0
84-85	0.0	6.281856866634922E-5	0.0	2.496786830212716	0.0
86-87	0.0	6.281856866634922E-5	0.0	2.7422189779921426	0.0
88	0.0	6.281856866634922E-5	0.0	2.9456569126181145	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8750	0.0	66.695305	1
GTATCAA	17545	0.0	45.61816	1
TGGTATC	2645	0.0	36.444542	2
GTGGTAT	2825	0.0	34.62394	1
ATCAACG	23485	0.0	33.91296	3
TATCAAC	23690	0.0	33.6441	2
TCAACGC	23755	0.0	33.487923	4
CAACGCA	24520	0.0	32.462303	5
AACGCAG	25390	0.0	31.356663	6
ACGCAGA	28430	0.0	27.887999	7
CGCAGAG	28550	0.0	27.770782	8
GTACATG	15535	0.0	26.123486	1
CATGGGT	7315	0.0	26.094835	4
GCAGAGT	30390	0.0	25.88832	9
TACATGG	15580	0.0	25.47295	2
ACATGGG	15570	0.0	24.428837	3
AGAGTAC	29640	0.0	22.373192	10-11
CAGAGTA	30580	0.0	22.000523	10-11
GAGTACT	22705	0.0	21.87926	12-13
AGTACTT	23035	0.0	21.565817	12-13
>>END_MODULE