FastQCFastQC Report
Wed 25 May 2016
SRR2431403_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431403_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3362018
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA349061.0382454823263885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40050.050.879041
GTATCAA71750.038.0416761
TCAACGC82150.032.3047834
ATCAACG82600.032.132143
CAACGCA89200.029.8038485
TATCAAC90800.029.7486082
AACGCAG94100.028.4154876
GTACATG151950.026.7897661
TACATGG153550.025.9888212
CATGGGT74900.025.8199424
CGCAGAG103700.025.241518
ACGCAGA105950.025.0603087
ACATGGG154750.024.6013223
GCAGAGT117750.021.3918959
ATGGGTA36650.020.670015
GTACGAC2109.42533E-720.1680453
ATGGGTG43250.019.691615
CAGAGTA116150.018.61161210-11
AGAGTAC107750.017.44568610-11
CATGGGG63300.017.245654
Produced by FastQC (version 0.11.5)