##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431403_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3362018
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.62111981553936	31.0	31.0	34.0	26.0	34.0
2	30.75535050674922	33.0	31.0	34.0	26.0	34.0
3	30.889439318885266	34.0	31.0	34.0	27.0	34.0
4	34.085998349800626	37.0	35.0	37.0	30.0	37.0
5	34.24215099383763	37.0	35.0	37.0	31.0	37.0
6	34.2367155678524	37.0	35.0	37.0	30.0	37.0
7	34.170584452552006	37.0	35.0	37.0	30.0	37.0
8	34.093717523225635	37.0	35.0	37.0	30.0	37.0
9	35.547788560323	39.0	37.0	39.0	29.0	39.0
10-11	35.578541518814	39.0	37.0	39.0	30.0	39.0
12-13	35.511852554031535	39.0	37.0	39.0	29.5	39.0
14-15	36.87101898324161	41.0	38.0	41.0	29.5	41.0
16-17	36.903404592122946	41.0	38.0	41.0	30.0	41.0
18-19	36.924955785483604	41.0	38.0	41.0	30.0	41.0
20-21	37.001750882951846	41.0	38.0	41.0	30.0	41.0
22-23	36.984943269191305	40.0	38.0	41.0	29.5	41.0
24-25	37.0383353093291	40.5	38.0	41.0	30.0	41.0
26-27	37.07297283952674	40.0	38.0	41.0	30.0	41.0
28-29	37.04027030789246	40.0	38.0	41.0	30.0	41.0
30-31	36.97094185694425	40.0	38.0	41.0	30.0	41.0
32-33	36.90796242018931	40.0	38.0	41.0	30.0	41.0
34-35	36.827670464584074	40.0	37.5	41.0	30.0	41.0
36-37	36.69501992553282	40.0	37.0	41.0	29.0	41.0
38-39	36.56852209000665	40.0	37.0	41.0	29.0	41.0
40-41	36.504448816157435	40.0	37.0	41.0	29.0	41.0
42-43	36.34912781549653	40.0	36.5	41.0	28.0	41.0
44-45	36.213944273944996	40.0	36.0	41.0	28.0	41.0
46-47	36.090700436464054	40.0	36.0	41.0	27.5	41.0
48-49	35.956171858687256	40.0	35.5	41.0	27.0	41.0
50-51	35.82903452628749	40.0	35.0	41.0	27.0	41.0
52-53	35.67309603934304	40.0	35.0	41.0	27.0	41.0
54-55	35.51655285605253	40.0	35.0	41.0	27.0	41.0
56-57	35.332767700827304	39.0	35.0	41.0	27.0	41.0
58-59	35.11987963776518	39.0	35.0	41.0	26.5	41.0
60-61	34.88473351421676	39.0	34.0	41.0	26.0	41.0
62-63	34.77905264040823	39.0	34.0	41.0	26.0	41.0
64-65	34.52324035742819	38.0	34.0	40.5	26.0	41.0
66-67	34.2585509655213	37.5	34.0	40.0	25.0	41.0
68-69	33.95809436475355	37.0	34.0	40.0	25.0	41.0
70-71	33.62356447823896	36.5	33.5	40.0	24.0	41.0
72-73	33.27466197385023	36.0	33.0	39.0	23.0	41.0
74-75	32.99057426224368	36.0	33.0	39.0	22.5	41.0
76-77	32.72305294022816	35.0	33.0	39.0	22.0	40.5
78-79	32.36851334525871	35.0	33.0	37.5	21.5	39.5
80-81	32.037508276279304	35.0	33.0	37.0	20.0	39.0
82-83	31.70972656898327	35.0	32.0	37.0	20.0	39.0
84-85	31.40592510212616	35.0	32.0	36.0	18.5	38.0
86-87	31.120723178757522	35.0	32.0	36.0	17.5	37.0
88-89	30.851217334350977	35.0	32.0	35.5	13.5	37.0
90-91	30.61780261140779	35.0	32.0	35.0	7.5	36.5
92-93	30.3875136896947	35.0	31.5	35.0	2.0	36.0
94-95	30.18041917086702	35.0	31.0	35.0	2.0	36.0
96-97	29.98148671422937	35.0	31.0	35.0	2.0	36.0
98-99	29.76882738283971	35.0	31.0	35.0	2.0	35.5
100	29.60181831269196	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.22864495429640286
1101	2	0.2530256004248592
1101	3	0.2957040375967992
1101	4	0.35046452060778677
1101	5	0.32842745860353517
1101	6	0.33814637393400204
1101	7	0.33387523300800126
1101	8	0.33141831547137457
1101	9	0.29250748873769794
1101	10-11	0.32885506298568146
1101	12-13	0.27329515531639004
1101	14-15	0.29006856901747824
1101	16-17	0.23646593408454208
1101	18-19	0.1864973753730581
1101	20-21	0.21880604832264794
1101	22-23	0.24139364550600106
1101	24-25	0.2964864719395379
1101	26-27	0.29618398090129716
1101	28-29	0.29213312806720637
1101	30-31	0.27511419360678957
1101	32-33	0.29412398658485017
1101	34-35	0.28055323452193903
1101	36-37	0.2821797564204118
1101	38-39	0.26446885273796994
1101	40-41	0.19288112045813932
1101	42-43	0.32021848156598054
1101	44-45	0.24229708981243903
1101	46-47	0.25640162278075707
1101	48-49	0.2666791230868739
1101	50-51	0.26346265237070554
1101	52-53	0.19860673232879122
1101	54-55	0.1989581566320453
1101	56-57	0.15128356581693225
1101	58-59	0.24772667522042013
1101	60-61	0.25695855402395296
1101	62-63	0.2433599352400435
1101	64-65	0.28121529017030156
1101	66-67	0.2553947878515075
1101	68-69	0.2940569887651705
1101	70-71	0.3193692075954573
1101	72-73	0.22699009988600238
1101	74-75	0.2326015331544724
1101	76-77	0.257469372265831
1101	78-79	0.24836997668146665
1101	80-81	0.24274394844935898
1101	82-83	0.26987573067170345
1101	84-85	0.21248484591300354
1101	86-87	0.1816799151742643
1101	88-89	0.14427525964729426
1101	90-91	0.11787805614040181
1101	92-93	0.04364186185923202
1101	94-95	0.05403305925932855
1101	96-97	0.11296661173820866
1101	98-99	0.07967757449408452
1101	100	0.09508653982992143
1102	1	0.08237087568056012
1102	2	0.18039653283690882
1102	3	0.19227240642186771
1102	4	0.20470465116041225
1102	5	0.2559411351330212
1102	6	0.20782412430757802
1102	7	0.15555844695179388
1102	8	0.13504344229587417
1102	9	0.15931705600134194
1102	10-11	0.2728183907874637
1102	12-13	0.11390239678979697
1102	14-15	0.16119153838025824
1102	16-17	0.15436624635685803
1102	18-19	0.07372000342787999
1102	20-21	0.097802177279533
1102	22-23	0.09549350953397351
1102	24-25	0.10987685684531101
1102	26-27	0.14517254928820478
1102	28-29	0.12500660714037082
1102	30-31	0.2286325970355776
1102	32-33	0.20697755102803228
1102	34-35	0.206243902629609
1102	36-37	0.18775539206857417
1102	38-39	0.14909935367854388
1102	40-41	0.1583924638776253
1102	42-43	0.1747966514003565
1102	44-45	0.18864742612609575
1102	46-47	0.1688987615957558
1102	48-49	0.11821669978032645
1102	50-51	0.11855820443097542
1102	52-53	0.06827833218694224
1102	54-55	0.03475280452742169
1102	56-57	0.0034601223819734628
1102	58-59	0.0734869419519768
1102	60-61	0.009772577825010842
1102	62-63	0.06828440560676796
1102	64-65	0.13686058529550138
1102	66-67	0.1282051500312349
1102	68-69	-0.022188321955823653
1102	70-71	-0.006549733740449426
1102	72-73	0.002734397010755174
1102	74-75	0.0015878047694002362
1102	76-77	0.007299048628752303
1102	78-79	-0.0408666387320622
1102	80-81	-0.06783641157703357
1102	82-83	-0.17204140975851345
1102	84-85	-0.14444199494495535
1102	86-87	-0.22641443260204142
1102	88-89	-0.1656180899807751
1102	90-91	-0.17574670208783516
1102	92-93	-0.20300856310009507
1102	94-95	-0.20253201827932799
1102	96-97	-0.19668145620451227
1102	98-99	-0.212529131332456
1102	100	-0.17044020813134608
1103	1	-0.3312594199155079
1103	2	-0.34963880285213733
1103	3	-0.20386057521352896
1103	4	-0.19282843762380253
1103	5	-0.2075170197409335
1103	6	-0.20740754841426678
1103	7	-0.2076753101951212
1103	8	-0.24899577534210948
1103	9	-0.27549269629752615
1103	10-11	-0.34920647300765495
1103	12-13	-0.2976921444756684
1103	14-15	-0.31970245162504085
1103	16-17	-0.3279724961375976
1103	18-19	-0.21018154554586488
1103	20-21	-0.3643645562331699
1103	22-23	-0.3992412678687529
1103	24-25	-0.3264446159532355
1103	26-27	-0.2549704621790596
1103	28-29	-0.24565364621096109
1103	30-31	-0.21030245790014845
1103	32-33	-0.2520508346257344
1103	34-35	-0.24120633611317288
1103	36-37	-0.37329747679708447
1103	38-39	-0.32509820122058386
1103	40-41	-0.2869030882754515
1103	42-43	-0.3710881460561737
1103	44-45	-0.3852062132742162
1103	46-47	-0.5183741692756882
1103	48-49	-0.5188865787978187
1103	50-51	-0.5528118366427393
1103	52-53	-0.5470361905763284
1103	54-55	-0.5329800319008271
1103	56-57	-0.4591120786479408
1103	58-59	-0.506121555007752
1103	60-61	-0.49370024414108116
1103	62-63	-0.4023009021240469
1103	64-65	-0.4486440241529124
1103	66-67	-0.41613572372931174
1103	68-69	-0.40920048012621635
1103	70-71	-0.5131938386526045
1103	72-73	-0.43666467670821163
1103	74-75	-0.40496327681885447
1103	76-77	-0.3979401186551428
1103	78-79	-0.4116505816328697
1103	80-81	-0.4031778093714067
1103	82-83	-0.4513975507616763
1103	84-85	-0.49025528666785334
1103	86-87	-0.5001811233723146
1103	88-89	-0.5418963373071755
1103	90-91	-0.5261376049776345
1103	92-93	-0.5512301415836731
1103	94-95	-0.5251593746018415
1103	96-97	-0.463940905646961
1103	98-99	-0.5944831920091325
1103	100	-0.43586553916556525
1104	1	-0.21614827620333443
1104	2	-0.2086911238829039
1104	3	-0.11164983591471156
1104	4	-0.06121673719665921
1104	5	-0.0896741566741639
1104	6	-0.0787030618767588
1104	7	-0.10110060396508658
1104	8	-0.10303783127818633
1104	9	-0.17279625393088338
1104	10-11	-0.06570972213697246
1104	12-13	-0.11168258661674457
1104	14-15	-0.16251196691413128
1104	16-17	-0.2422274944882261
1104	18-19	-0.21569024649622293
1104	20-21	-0.22154556449358154
1104	22-23	-0.13457312171686908
1104	24-25	-0.14327438922227032
1104	26-27	-0.17774146110293998
1104	28-29	-0.1867163145928501
1104	30-31	-0.16860630894306894
1104	32-33	-0.2212780862685335
1104	34-35	-0.20029401893235388
1104	36-37	-0.290635607574643
1104	38-39	-0.2676025408837077
1104	40-41	-0.24786477627932868
1104	42-43	-0.3200477505129484
1104	44-45	-0.31928607325171754
1104	46-47	-0.379976842694731
1104	48-49	-0.3882817353352124
1104	50-51	-0.42510146580188035
1104	52-53	-0.5231136863172239
1104	54-55	-0.3855981610502184
1104	56-57	-0.4224847918977517
1104	58-59	-0.44198523488943664
1104	60-61	-0.4952336483110571
1104	62-63	-0.3837229887409208
1104	64-65	-0.44487104005045097
1104	66-67	-0.3626101025447923
1104	68-69	-0.3724695884240248
1104	70-71	-0.40113402719793356
1104	72-73	-0.39038964255482966
1104	74-75	-0.3664273438994101
1104	76-77	-0.3726167533322524
1104	78-79	-0.31191511664231086
1104	80-81	-0.2973034700729258
1104	82-83	-0.40892655887623164
1104	84-85	-0.3908012743768765
1104	86-87	-0.29281425372163383
1104	88-89	-0.35073678440908296
1104	90-91	-0.3668341686089249
1104	92-93	-0.4332008659236912
1104	94-95	-0.4216628611724147
1104	96-97	-0.39965355592395824
1104	98-99	-0.38052830245748126
1104	100	-0.32981210131284655
1105	1	-0.17964003250318328
1105	2	-0.11650967658079736
1105	3	-0.07427163372542722
1105	4	0.05127781650718077
1105	5	0.028317508034767513
1105	6	-0.04455155083358875
1105	7	0.005331769336727632
1105	8	-0.012508332275338319
1105	9	-0.05921668419086501
1105	10-11	-0.05789272182752114
1105	12-13	-0.07511100546770422
1105	14-15	-0.009073478236736321
1105	16-17	-0.16225796269893777
1105	18-19	-0.08540894335925486
1105	20-21	-0.10503125612920883
1105	22-23	-0.10540643348925727
1105	24-25	-0.20835962052297674
1105	26-27	-0.21906728363596528
1105	28-29	-0.16235902928558943
1105	30-31	-0.19452105383907536
1105	32-33	-0.1602125061357924
1105	34-35	-0.20755288384660986
1105	36-37	-0.22626085272265328
1105	38-39	-0.2685840643563395
1105	40-41	-0.29575430417729365
1105	42-43	-0.3645748050214479
1105	44-45	-0.39430338238296514
1105	46-47	-0.3894798403568345
1105	48-49	-0.5307839578380182
1105	50-51	-0.40471177490312726
1105	52-53	-0.4039986098310422
1105	54-55	-0.42803663213388177
1105	56-57	-0.46634908002933173
1105	58-59	-0.41749475465289265
1105	60-61	-0.5003059223428039
1105	62-63	-0.37078186462539264
1105	64-65	-0.3713696899899688
1105	66-67	-0.44041933056271887
1105	68-69	-0.4203836440595552
1105	70-71	-0.46456337748196574
1105	72-73	-0.40743165950070903
1105	74-75	-0.4347416466751852
1105	76-77	-0.41079754035180827
1105	78-79	-0.43489998706444766
1105	80-81	-0.4731512035340373
1105	82-83	-0.39090198353208905
1105	84-85	-0.44457404403451406
1105	86-87	-0.4161355784678449
1105	88-89	-0.4260827859221443
1105	90-91	-0.4752601191622503
1105	92-93	-0.6028735085959518
1105	94-95	-0.5632399636497176
1105	96-97	-0.5615067144652528
1105	98-99	-0.5503384867466892
1105	100	-0.4745673569239557
1106	1	-0.379796669960097
1106	2	-0.314726230580888
1106	3	-0.22622151456749506
1106	4	-0.19539479430888917
1106	5	-0.14984096175447803
1106	6	-0.1632203376517367
1106	7	-0.17830434410693385
1106	8	-0.17780490737501253
1106	9	-0.18112697715883996
1106	10-11	-0.2029283042408352
1106	12-13	-0.17560236344968416
1106	14-15	-0.26480597461452504
1106	16-17	-0.22577187639423357
1106	18-19	-0.34723790797077925
1106	20-21	-0.38116381978102964
1106	22-23	-0.26602182998963286
1106	24-25	-0.25470280180437754
1106	26-27	-0.35695695099562386
1106	28-29	-0.358985530025727
1106	30-31	-0.28032512544729826
1106	32-33	-0.4085586592236581
1106	34-35	-0.3342431742153664
1106	36-37	-0.3674645107886718
1106	38-39	-0.3996769616745084
1106	40-41	-0.35822335791887383
1106	42-43	-0.3866290813286355
1106	44-45	-0.3614632310485675
1106	46-47	-0.38926991075717865
1106	48-49	-0.42528585434624233
1106	50-51	-0.46572154130691246
1106	52-53	-0.5289370995530405
1106	54-55	-0.5111735416402396
1106	56-57	-0.577187522957253
1106	58-59	-0.5611858420902323
1106	60-61	-0.544063458986443
1106	62-63	-0.47801555378956806
1106	64-65	-0.5067162820489699
1106	66-67	-0.481263422578472
1106	68-69	-0.46026749173214654
1106	70-71	-0.44880546020891643
1106	72-73	-0.4419945009757882
1106	74-75	-0.5293179992877057
1106	76-77	-0.5869152771598394
1106	78-79	-0.5280117009952789
1106	80-81	-0.5038758036619093
1106	82-83	-0.4896400320297509
1106	84-85	-0.4424934576237902
1106	86-87	-0.46792095577983517
1106	88-89	-0.555313395448259
1106	90-91	-0.5139324306249478
1106	92-93	-0.4977051162885324
1106	94-95	-0.5247581881945251
1106	96-97	-0.4919595830068779
1106	98-99	-0.53467109972744
1106	100	-0.592150867059523
1107	1	-0.38049566258697354
1107	2	-0.45686529217397975
1107	3	-0.4082790352054033
1107	4	-0.36541427580764463
1107	5	-0.33152938260300857
1107	6	-0.37934925391139274
1107	7	-0.45039789712170375
1107	8	-0.3943929806044082
1107	9	-0.34777474000224373
1107	10-11	-0.38818499334286116
1107	12-13	-0.4018807714605117
1107	14-15	-0.45494590937415325
1107	16-17	-0.4408625212828454
1107	18-19	-0.4616529418329165
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2216	70-71	0.10411484415001127
2216	72-73	0.14574658476833946
2216	74-75	0.11152285991220623
2216	76-77	0.1594841402240803
2216	78-79	0.21395721831412118
2216	80-81	0.14988654000308088
2216	82-83	0.10680110869132164
2216	84-85	0.14659674509366027
2216	86-87	0.13992738276240857
2216	88-89	0.20396263912652657
2216	90-91	0.07817656496011338
2216	92-93	0.14574563029000132
2216	94-95	0.15434073749571553
2216	96-97	0.14217294084766507
2216	98-99	0.1702589809200319
2216	100	0.19980597359231922
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	80314.0
3	16125.0
4	11348.0
5	10064.0
6	6771.0
7	5937.0
8	6581.0
9	6914.0
10	7095.0
11	7839.0
12	9224.0
13	10439.0
14	11486.0
15	11796.0
16	11615.0
17	11462.0
18	10877.0
19	10021.0
20	9500.0
21	10001.0
22	11810.0
23	18063.0
24	30809.0
25	38553.0
26	41915.0
27	31900.0
28	30768.0
29	37679.0
30	43913.0
31	49456.0
32	63437.0
33	84277.0
34	117821.0
35	191432.0
36	306650.0
37	570580.0
38	917917.0
39	507234.0
40	2395.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.617343248971736	22.210958514692432	14.127907444506127	22.0437907918297
2	14.856383820307478	22.99584074886186	39.95029398417763	22.19748144665304
3	16.99086441209321	29.339285054421353	30.447857177766096	23.221993355719338
4	11.558444035096398	19.91791452598231	39.26121881493939	29.262422623981905
5	11.809469032086948	40.70396385710923	34.78881382298685	12.697753287816974
6	27.225635530154197	40.981157349526704	18.777817467404894	13.01538965291421
7	24.257579591289982	35.92230639855924	23.243692976319824	16.57642103383095
8	23.279792722981288	37.850944488511814	21.344234921891626	17.525027866615275
9	24.324921175079123	19.342983793346267	21.62469516169451	34.7073998698801
10-11	21.72973719546372	30.01732701928535	29.32426113106948	18.92867465418145
12-13	22.724026190023675	28.283624150865442	28.636129498722067	20.356220160388816
14-15	20.68619726593835	29.79814365744667	26.2427383835717	23.272920693043282
16-17	19.841208186930853	32.637878767585036	26.868899276582002	20.652013768902105
18-19	19.854077301763578	31.56414761695411	28.794551235017874	19.78722384626444
20-21	21.693178784075048	30.63140025711907	27.92937292177216	19.74604803703372
22-23	21.159473824499724	30.649081446117936	28.01580782720808	20.17563690217426
24-25	20.79740779512866	30.746729308839626	28.004685661524505	20.45117723450721
26-27	20.563523873450844	30.580460993277242	28.139580917908546	20.716434215363368
28-29	20.440882031954256	30.653130068057543	28.31413477295623	20.591853127031968
30-31	20.756175021529263	31.030939926135204	27.91749432410316	20.295390728232373
32-33	20.39361932817471	30.76864803417305	28.344842274399127	20.492890363253107
34-35	20.666809048647757	30.372651348449143	28.265154937144803	20.695384665758297
36-37	20.85895944550995	30.67982784194384	27.897062402457784	20.564150310088422
38-39	20.711523010686495	30.691468159761282	28.12197945575951	20.475029373792715
40-41	20.586552600715592	30.44800354818929	28.31316640174314	20.65227744935198
42-43	20.4978152692859	30.517868324373982	28.27205415590004	20.71226225044008
44-45	20.748864763257778	30.581668721351146	28.128551103590333	20.540915411800746
46-47	20.701224149833106	30.795823905556823	27.97134590992022	20.53160603468985
48-49	20.63203251078925	30.468653946511758	28.11771854089644	20.78159500180255
50-51	20.584300153948078	30.429713541670544	28.11662247772903	20.86936382665235
52-53	20.65077383842374	30.477082528972616	28.03088328407198	20.84126034853167
54-55	20.772659095587517	30.784133449609964	27.879792708478924	20.5634147463236
56-57	20.70263140739709	30.458583038866106	27.846806790502153	20.991978763234652
58-59	20.60045686806182	30.225958721120705	28.165348907790023	21.008235503027453
60-61	20.79894061215459	30.63023425872991	27.797217186862078	20.77360794225342
62-63	20.712593117789112	30.57406724453497	27.930395652563455	20.782943985112464
64-65	20.732410456806615	30.300780236083032	28.059819091911404	20.90699021519895
66-67	20.55339392127239	30.411721255394642	27.870620644122535	21.164264179210438
68-69	21.03223096794108	30.541871921182267	27.73317605029366	20.69272106058299
70-71	20.98417442804974	30.303548929677714	27.884247619348102	20.828029022924444
72-73	20.796220354510673	30.4735480176416	28.04061032458615	20.68962130326158
74-75	20.80362854124629	30.539631805933286	27.896598599768364	20.760141053052056
76-77	20.82707419330209	30.550283895689752	27.717250638912276	20.905391272095887
78-79	20.992358402743207	30.516888681521237	27.86458693777259	20.62616597796297
80-81	20.837156716048028	30.44674822323405	27.898779003195195	20.817316057522728
82-83	20.76669022869456	30.45967387950501	28.072645467986078	20.700990423814346
84-85	20.7864844923446	30.33923475293775	27.889994041671386	20.98428671304627
86-87	20.988867103456887	30.442831697342154	27.741894576469324	20.826406622731636
88-89	20.863804821328287	30.258814584756966	27.922274802227125	20.95510579168762
90-91	20.790164309214248	30.222911127278618	27.852921284229105	21.134003279278033
92-93	20.854931321263315	30.44039729234938	27.751231064848213	20.95344032153909
94-95	20.728825884202596	30.330955179605724	27.89552296115411	21.044695975037573
96-97	20.70997700946886	30.408787359613104	27.807767727771154	21.07346790314688
98-99	20.737607243931066	30.37542855152635	27.807498092597793	21.07946611194479
100	20.827425117362996	30.435218812136743	27.64143026112057	21.095925809379693
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	32936.0
1	22867.5
2	10639.0
3	6802.0
4	4160.5
5	2727.0
6	2130.0
7	2193.5
8	2610.5
9	2633.0
10	1960.5
11	1370.5
12	1248.0
13	1263.5
14	1348.0
15	1444.0
16	1660.0
17	2095.0
18	2735.5
19	3710.5
20	5084.0
21	7069.0
22	10171.0
23	14825.0
24	20183.0
25	26344.0
26	34710.0
27	44459.5
28	56167.5
29	67782.5
30	76355.5
31	84786.5
32	93066.5
33	100747.5
34	107596.0
35	112818.5
36	115808.0
37	117518.5
38	118523.0
39	117098.5
40	115515.5
41	113398.5
42	108969.5
43	105315.5
44	102183.5
45	98586.0
46	96359.0
47	93771.5
48	91013.5
49	89026.0
50	87126.0
51	83669.0
52	78611.0
53	74579.0
54	71675.5
55	69217.0
56	64469.0
57	58123.0
58	53737.0
59	49857.0
60	44074.0
61	37249.5
62	31614.5
63	25892.5
64	20173.0
65	16120.0
66	12693.5
67	9743.0
68	7583.0
69	5928.5
70	4663.5
71	3681.5
72	2953.5
73	2382.5
74	1969.5
75	1611.0
76	1224.0
77	928.5
78	719.0
79	550.0
80	381.0
81	274.0
82	213.0
83	155.0
84	121.5
85	106.0
86	96.5
87	77.0
88	62.5
89	51.0
90	50.0
91	44.0
92	28.0
93	27.5
94	25.0
95	22.5
96	25.5
97	23.0
98	20.5
99	20.5
100	34.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18164685614413725
2	0.1750436791236692
3	0.18887465801789283
4	0.17858321995896512
5	0.19178957399990126
6	0.07786989837651077
7	0.07935710040814772
8	0.06731076395188842
9	0.06067784289078762
10-11	0.07510370259766605
12-13	0.0741965093583675
14-15	0.0714154415592064
16-17	0.06760820435821581
18-19	0.07230776277818858
20-21	0.07391394097235648
22-23	0.07187647418901386
24-25	0.07159390580300284
26-27	0.07534165492272796
28-29	0.07241186692040316
30-31	0.07843503514853281
32-33	0.07520780673988063
34-35	0.07467241400849134
36-37	0.08612386965209586
38-39	0.08123097496801028
40-41	0.07608525593854643
42-43	0.07969915687542423
44-45	0.09003521099530104
46-47	0.08051711799282456
48-49	0.08587104530671757
50-51	0.082346376491738
52-53	0.08105251072421385
54-55	0.08291151326376003
56-57	0.07617448806044465
58-59	0.0845474354985607
60-61	0.08088891850073378
62-63	0.0722482746969231
64-65	0.07501447047576783
66-67	0.07580268755253541
68-69	0.07345290834254903
70-71	0.06927387063364919
72-73	0.06638869869227351
74-75	0.07230776277818858
76-77	0.07726014554353962
78-79	0.07273905136736329
80-81	0.08192995992287964
82-83	0.08326844175135291
84-85	0.08490436398615356
86-87	0.08148379931338857
88-89	0.08323869771072016
90-91	0.08155815941497041
92-93	0.07217391459534125
94-95	0.07165339388426832
96-97	0.07061235246212245
98-99	0.08140943921180673
100	0.0890834016950534
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3362018.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.91929327889176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.16390419487564	52.3067322864008
2	14.249856395595097	19.071806386378395
3	4.032703516705556	8.095970080237118
4	1.5288720316925277	4.0924414349890945
5	0.6661967046086068	2.229070632856729
6	0.37821432390418364	1.5185901158173096
7	0.20905566964920883	0.9792900368209361
8	0.14581416492359237	0.7806224693390855
9	0.09713330628192172	0.5850082989205505
>10	0.48399847855825906	5.656744053574826
>50	0.02986033647897478	1.3816045516581417
>100	0.013282610727418915	1.571449424715907
>500	7.536208840441233E-4	0.31955947036600507
>1k	3.103144756882539E-4	0.3755980681163106
>5k	0.0	0.0
>10k+	4.433063938403626E-5	1.035512689808699
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	34906	1.0382454823263885	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.9744040632739027E-5	0.0
4	0.0	0.0	0.0	1.1897616253095611E-4	0.0
5	0.0	0.0	0.0	1.1897616253095611E-4	0.0
6	0.0	0.0	0.0	1.1897616253095611E-4	0.0
7	0.0	0.0	0.0	1.1897616253095611E-4	0.0
8	0.0	0.0	0.0	1.1897616253095611E-4	0.0
9	0.0	0.0	0.0	1.1897616253095611E-4	0.0
10-11	0.0	0.0	0.0	1.4872020316369515E-4	0.0
12-13	0.0	0.0	0.0	1.784642437964342E-4	0.0
14-15	0.0	0.0	0.0	2.8256838601102075E-4	0.0
16-17	0.0	0.0	0.0	3.718005079092378E-4	0.0
18-19	0.0	0.0	0.0	4.164165688583464E-4	0.0
20-21	0.0	0.0	0.0	4.164165688583464E-4	0.0
22-23	0.0	0.0	0.0	5.502647517056721E-4	0.0
24-25	0.0	0.0	0.0	8.774491986658013E-4	0.0
26-27	0.0	0.0	0.0	0.0014425859706878427	0.0
28-29	0.0	0.0	0.0	0.0031528683070703367	0.0
30-31	0.0	0.0	0.0	0.006618049040784434	0.0
32-33	0.0	1.4872020316369514E-5	0.0	0.013429434345681671	0.0
34-35	0.0	2.9744040632739027E-5	0.0	0.024033184831253135	0.0
36-37	0.0	2.9744040632739027E-5	0.0	0.040094966772932214	0.0
38-39	2.9744040632739027E-5	2.9744040632739027E-5	0.0	0.06314659826330496	0.0
40-41	2.9744040632739027E-5	2.9744040632739027E-5	0.0	0.08764081572436555	0.0
42-43	2.9744040632739027E-5	2.9744040632739027E-5	0.0	0.11262580985586633	0.0
44-45	2.9744040632739027E-5	2.9744040632739027E-5	0.0	0.14290524321999465	0.0
46-47	2.9744040632739027E-5	2.9744040632739027E-5	0.0	0.1783898836948523	0.0
48-49	2.9744040632739027E-5	2.9744040632739027E-5	0.0	0.22061154937302535	0.0
50-51	2.9744040632739027E-5	2.9744040632739027E-5	0.0	0.26307116737626035	0.0
52-53	2.9744040632739027E-5	2.9744040632739027E-5	0.0	0.30495077658715686	0.0
54-55	2.9744040632739027E-5	2.9744040632739027E-5	0.0	0.35109865562885145	0.0
56-57	2.9744040632739027E-5	2.9744040632739027E-5	0.0	0.40933748718775453	0.0
58-59	2.9744040632739027E-5	2.9744040632739027E-5	0.0	0.4778528847852689	0.0
60-61	2.9744040632739027E-5	2.9744040632739027E-5	0.0	0.5532540277892624	0.0
62-63	2.9744040632739027E-5	2.9744040632739027E-5	0.0	0.627019248558455	0.0
64-65	2.9744040632739027E-5	2.9744040632739027E-5	0.0	0.7090681846438657	0.0
66-67	2.9744040632739027E-5	2.9744040632739027E-5	0.0	0.8063758135738714	0.0
68-69	2.9744040632739027E-5	4.461606094910854E-5	0.0	0.9159379872445657	0.0
70-71	2.9744040632739027E-5	5.9488081265478054E-5	0.0	1.041205014369346	0.0
72-73	2.9744040632739027E-5	5.9488081265478054E-5	0.0	1.1736254832663002	0.0
74-75	2.9744040632739027E-5	5.9488081265478054E-5	0.0	1.3092285645109574	0.0
76-77	2.9744040632739027E-5	5.9488081265478054E-5	0.0	1.457963639694969	0.0
78-79	2.9744040632739027E-5	5.9488081265478054E-5	0.0	1.6295272660646076	0.0
80-81	2.9744040632739027E-5	5.9488081265478054E-5	0.0	1.826075886565747	0.0
82-83	2.9744040632739027E-5	5.9488081265478054E-5	0.0	2.0359349652500374	0.0
84-85	2.9744040632739027E-5	5.9488081265478054E-5	0.0	2.2488130640585506	0.0
86-87	2.9744040632739027E-5	5.9488081265478054E-5	0.0	2.4771580639960877	0.0
88	2.9744040632739027E-5	5.9488081265478054E-5	0.0	2.6656906655467045	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4005	0.0	50.87904	1
GTATCAA	7175	0.0	38.041676	1
TCAACGC	8215	0.0	32.304783	4
ATCAACG	8260	0.0	32.13214	3
CAACGCA	8920	0.0	29.803848	5
TATCAAC	9080	0.0	29.748608	2
AACGCAG	9410	0.0	28.415487	6
GTACATG	15195	0.0	26.789766	1
TACATGG	15355	0.0	25.988821	2
CATGGGT	7490	0.0	25.819942	4
CGCAGAG	10370	0.0	25.24151	8
ACGCAGA	10595	0.0	25.060308	7
ACATGGG	15475	0.0	24.601322	3
GCAGAGT	11775	0.0	21.391895	9
ATGGGTA	3665	0.0	20.67001	5
GTACGAC	210	9.42533E-7	20.168045	3
ATGGGTG	4325	0.0	19.69161	5
CAGAGTA	11615	0.0	18.611612	10-11
AGAGTAC	10775	0.0	17.445686	10-11
CATGGGG	6330	0.0	17.24565	4
>>END_MODULE