FastQCFastQC Report
Wed 25 May 2016
SRR2431403_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431403_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3362018
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296210.8810482275823628No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238910.7106148747567681No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160580.47762980448052333No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104080.3095759749055478No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58200.17311031648254116No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51420.15294385693354406No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46910.13952929460817878No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46760.13908313399868769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95250.066.794741
GTATCAA191900.046.36131
GTGGTAT27550.037.7207371
TGGTATC28600.036.0054472
ATCAACG251300.035.2115333
TATCAAC254450.034.796692
TCAACGC254150.034.779164
CAACGCA262150.033.735745
AACGCAG269900.032.9285056
CGCAGAG302200.029.253498
ACGCAGA303800.029.1922447
GCAGAGT323650.027.0678449
GTACATG146650.025.0425431
TATAACG3200.024.9798052
TACATGG147450.024.3315542
CATGGGT72800.023.960394
ACATGGG147400.023.7638363
GAGTACT243350.023.15640812-13
CAGAGTA319900.023.10988810-11
ATAACGC3100.022.7503093
Produced by FastQC (version 0.11.5)