##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431403_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3362018
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64595400738485	34.0	31.0	34.0	31.0	34.0
2	32.78029980803196	34.0	31.0	34.0	31.0	34.0
3	32.86704532813328	34.0	31.0	34.0	31.0	34.0
4	36.32948336386063	37.0	37.0	37.0	35.0	37.0
5	36.23778337891112	37.0	37.0	37.0	35.0	37.0
6	36.27899523440981	37.0	37.0	37.0	35.0	37.0
7	36.23488660679389	37.0	37.0	37.0	35.0	37.0
8	36.25443558005936	37.0	37.0	37.0	35.0	37.0
9	38.053824815929005	39.0	39.0	39.0	35.0	39.0
10-11	38.03445951211445	39.0	39.0	39.0	35.0	39.0
12-13	38.01509346469888	39.0	39.0	39.0	35.0	39.0
14-15	39.48005320019108	41.0	39.0	41.0	36.0	41.0
16-17	39.509345577566805	41.0	39.0	41.0	36.5	41.0
18-19	39.580459265833795	41.0	40.0	41.0	37.0	41.0
20-21	39.57418981694923	41.0	40.0	41.0	37.0	41.0
22-23	39.501607516675996	41.0	39.5	41.0	36.0	41.0
24-25	39.446395587412084	41.0	39.0	41.0	36.0	41.0
26-27	39.32132382991406	41.0	39.0	41.0	35.5	41.0
28-29	39.232762436132106	41.0	39.0	41.0	35.0	41.0
30-31	39.03669804861247	41.0	39.0	41.0	35.0	41.0
32-33	38.78664926838583	41.0	38.0	41.0	35.0	41.0
34-35	38.640975449863745	41.0	38.0	41.0	35.0	41.0
36-37	38.48865859730674	41.0	38.0	41.0	35.0	41.0
38-39	38.33337685877946	41.0	38.0	41.0	35.0	41.0
40-41	38.186050907520425	40.0	38.0	41.0	34.0	41.0
42-43	38.0017725961015	40.0	38.0	41.0	34.0	41.0
44-45	37.74325821575018	40.0	38.0	41.0	33.0	41.0
46-47	37.35517864568244	40.0	38.0	41.0	33.0	41.0
48-49	37.0027032871329	40.0	37.0	41.0	31.5	41.0
50-51	36.83164605305504	40.0	37.0	41.0	31.0	41.0
52-53	36.76607591036098	40.0	37.0	41.0	31.0	41.0
54-55	36.62619578479354	40.0	36.5	41.0	31.0	41.0
56-57	36.478398390490476	40.0	36.0	41.0	31.0	41.0
58-59	36.306471291944305	40.0	35.5	41.0	30.5	41.0
60-61	36.15713761794255	39.5	35.0	41.0	30.0	41.0
62-63	36.00124776250455	39.0	35.0	41.0	30.0	41.0
64-65	35.77213953048437	39.0	35.0	41.0	30.0	41.0
66-67	35.5157994395033	39.0	35.0	41.0	29.0	41.0
68-69	35.228449550240356	38.0	35.0	41.0	29.0	41.0
70-71	34.933411718795085	37.0	35.0	40.0	29.0	41.0
72-73	34.62095072066836	37.0	35.0	39.5	29.0	41.0
74-75	34.28724786720357	36.0	35.0	39.0	28.0	41.0
76-77	33.954624871133944	36.0	35.0	39.0	28.0	41.0
78-79	33.63367536997125	35.5	34.5	38.5	28.0	40.0
80-81	33.365728113293855	35.0	34.0	37.0	28.0	39.0
82-83	33.16144336526455	35.0	34.0	37.0	28.5	39.0
84-85	32.89460199201789	35.0	34.0	36.5	28.0	38.5
86-87	32.64793629897282	35.0	34.0	36.0	27.5	37.0
88-89	32.410636855602796	35.0	34.0	36.0	27.0	37.0
90-91	32.2251278845027	35.0	34.0	35.5	27.0	37.0
92-93	32.05967948416695	35.0	34.0	35.0	26.5	36.0
94-95	31.917666562165937	35.0	34.0	35.0	26.0	36.0
96-97	31.774962983541432	35.0	34.0	35.0	26.0	36.0
98-99	31.660186233387208	35.0	34.0	35.0	25.0	36.0
100	31.556480958757508	35.0	34.0	35.0	25.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.024469771850540667
1101	2	0.04722863435051039
1101	3	0.03153013933036419
1101	4	0.05456346526261058
1101	5	0.0317854141485725
1101	6	0.055987971876895415
1101	7	0.05074357935681206
1101	8	0.062263002421126146
1101	9	0.06479625984755444
1101	10-11	0.11938691071799212
1101	12-13	0.06344162173140688
1101	14-15	0.07353216232493764
1101	16-17	0.03365071586372892
1101	18-19	0.079241625673653
1101	20-21	0.09565123972949863
1101	22-23	0.060957471325579604
1101	24-25	0.11880695869431435
1101	26-27	0.09671751421537067
1101	28-29	0.07563295163225803
1101	30-31	0.07952312893985436
1101	32-33	0.06265118813069392
1101	34-35	0.04950404509126116
1101	36-37	0.021566971193372808
1101	38-39	0.016058718205549383
1101	40-41	0.02449301552742611
1101	42-43	-0.06318035074468753
1101	44-45	-0.05210098439820143
1101	46-47	-0.002173162821499375
1101	48-49	-0.062314448022817714
1101	50-51	-0.1693172742088649
1101	52-53	-0.03093978267349229
1101	54-55	-0.05848730632708765
1101	56-57	-0.037141258788416565
1101	58-59	9.148677037416064E-4
1101	60-61	0.0034201254916510493
1101	62-63	0.012655199608332168
1101	64-65	-0.042436838745693706
1101	66-67	0.03706455770605288
1101	68-69	0.030146073400288742
1101	70-71	0.07924554706132625
1101	72-73	0.02451203302106819
1101	74-75	-0.014413271178256082
1101	76-77	-0.04887192262599882
1101	78-79	-0.018886077985079908
1101	80-81	0.026701628585669823
1101	82-83	-0.08746763003593827
1101	84-85	-0.033393722492299105
1101	86-87	0.03756737681046474
1101	88-89	0.05037752363590897
1101	90-91	0.017289247833311094
1101	92-93	0.035080969806912776
1101	94-95	0.031370908855834045
1101	96-97	0.0033369688411823972
1101	98-99	0.03204080482846905
1101	100	0.07733479336317828
1102	1	0.009177764938662847
1102	2	0.007902716930502152
1102	3	0.03584762728426938
1102	4	0.044757457300065084
1102	5	0.0031026267854059597
1102	6	-0.055152537104120825
1102	7	-0.010644606315899807
1102	8	-0.030504156625809742
1102	9	0.04242911728312748
1102	10-11	0.014307680343499385
1102	12-13	-0.012723796619695804
1102	14-15	-9.79396797262666E-5
1102	16-17	-0.014473416055132304
1102	18-19	-0.04614399860278695
1102	20-21	0.016536421038445326
1102	22-23	0.008680546172968207
1102	24-25	0.0807392454750655
1102	26-27	0.0687465131783469
1102	28-29	0.021639945746287026
1102	30-31	0.02582875991709699
1102	32-33	0.01392626169564437
1102	34-35	0.06181535575435504
1102	36-37	0.046997874564560504
1102	38-39	-0.013778953392737492
1102	40-41	-0.08080063528899473
1102	42-43	-0.13051195550065842
1102	44-45	-0.10741561216683948
1102	46-47	-0.22936600847243227
1102	48-49	-0.223364346654499
1102	50-51	-0.20872882071272159
1102	52-53	-0.20797876111939217
1102	54-55	-0.19264223985327789
1102	56-57	-0.19339658072291144
1102	58-59	-0.22946553145992254
1102	60-61	-0.2412602872074956
1102	62-63	-0.24238224638123285
1102	64-65	-0.2791282535215842
1102	66-67	-0.2599721065812517
1102	68-69	-0.33597041988269893
1102	70-71	-0.29304775889156076
1102	72-73	-0.2942649211237267
1102	74-75	-0.19709666237859125
1102	76-77	-0.2438874014955701
1102	78-79	-0.2400381829696414
1102	80-81	-0.28032292940090997
1102	82-83	-0.2950603695937275
1102	84-85	-0.2663335184655722
1102	86-87	-0.24680817983291803
1102	88-89	-0.20564802547472283
1102	90-91	-0.22406640921951748
1102	92-93	-0.22480929804347483
1102	94-95	-0.2065823613101827
1102	96-97	-0.19120252968187046
1102	98-99	-0.25501174734854715
1102	100	-0.18822446757002353
1103	1	-0.07516606566081663
1103	2	-0.1075728218143297
1103	3	-0.06967200056388378
1103	4	0.00325266048759687
1103	5	-0.02697149911737995
1103	6	-0.01368506672373826
1103	7	-0.03298191430434372
1103	8	-0.00728052884792163
1103	9	0.004165864935956165
1103	10-11	0.02479384614311897
1103	12-13	-0.004362309885948434
1103	14-15	-0.031118034474225453
1103	16-17	0.002702231887809603
1103	18-19	-0.0194541358762379
1103	20-21	-0.09700070485556722
1103	22-23	-0.0590050832970519
1103	24-25	-0.04449056824407904
1103	26-27	-0.126032324052062
1103	28-29	-0.053134412312275
1103	30-31	-0.13320782291390998
1103	32-33	-0.09673599703654645
1103	34-35	-0.17505122933088302
1103	36-37	-0.19198607067829698
1103	38-39	-0.20281720493483846
1103	40-41	-0.22983958944627858
1103	42-43	-0.20081775412528913
1103	44-45	-0.23870255435624443
1103	46-47	-0.24537831594854964
1103	48-49	-0.2791373551063714
1103	50-51	-0.40415794294140994
1103	52-53	-0.3348300789620069
1103	54-55	-0.30725378147883475
1103	56-57	-0.33109635032368345
1103	58-59	-0.37306068165855777
1103	60-61	-0.3695889266810042
1103	62-63	-0.4370692313130604
1103	64-65	-0.494003349033342
1103	66-67	-0.40078716947995474
1103	68-69	-0.3677860800043078
1103	70-71	-0.4264842644348974
1103	72-73	-0.37918731142060835
1103	74-75	-0.3911769098162381
1103	76-77	-0.3381406679243213
1103	78-79	-0.35711985613061614
1103	80-81	-0.29587341376625886
1103	82-83	-0.3092023300657658
1103	84-85	-0.34334197326838733
1103	86-87	-0.3395932732660967
1103	88-89	-0.3226118642128242
1103	90-91	-0.32392047018894843
1103	92-93	-0.348098869184728
1103	94-95	-0.3493427900225541
1103	96-97	-0.29168601676106576
1103	98-99	-0.27554677417966644
1103	100	-0.24920048789902438
1104	1	-0.09027059493774914
1104	2	-0.08295376664401033
1104	3	-0.06062065473831524
1104	4	-0.055507480586435065
1104	5	-0.027454819292508148
1104	6	-0.08084744856491
1104	7	-0.0965678370901415
1104	8	-0.11563689257062748
1104	9	-0.05573920943827915
1104	10-11	-0.04952020300213178
1104	12-13	-0.06360795474674319
1104	14-15	-0.04246787534617624
1104	16-17	-0.009116343481558431
1104	18-19	-0.0671863173933005
1104	20-21	-0.017079522635505384
1104	22-23	-0.07478841899066424
1104	24-25	-0.0529666490046381
1104	26-27	-0.0695342350302397
1104	28-29	-0.0425636119910493
1104	30-31	-0.020902747352472772
1104	32-33	-0.04174869166418205
1104	34-35	-0.09041293717805132
1104	36-37	-0.16222329570223337
1104	38-39	-0.24718944415013056
1104	40-41	-0.34548177478784226
1104	42-43	-0.22468130087027305
1104	44-45	-0.14840722406272278
1104	46-47	-0.2502552747329858
1104	48-49	-0.2492133956715179
1104	50-51	-0.25772458634449436
1104	52-53	-0.18547799681412158
1104	54-55	-0.16080401599448635
1104	56-57	-0.15633561479848623
1104	58-59	-0.10685762612868643
1104	60-61	-0.1395772199786336
1104	62-63	-0.17070675093377474
1104	64-65	-0.14507268273074914
1104	66-67	-0.16897059116122648
1104	68-69	-0.18228680886419113
1104	70-71	-0.1831630844196539
1104	72-73	-0.17327500271983354
1104	74-75	-0.17163381889065477
1104	76-77	-0.21952173232263306
1104	78-79	-0.20236587920142313
1104	80-81	-0.1457001664431914
1104	82-83	-0.11273183087094907
1104	84-85	-0.09785816973068506
1104	86-87	-0.16151124324505162
1104	88-89	-0.14927382586775195
1104	90-91	-0.1269149861437313
1104	92-93	-0.15070383306397517
1104	94-95	-0.16713752883292443
1104	96-97	-0.1631808112853328
1104	98-99	-0.13885893990688558
1104	100	-0.08296857901541443
1105	1	-0.2002008099021495
1105	2	-0.14670539277091166
1105	3	-0.1556129600662146
1105	4	-0.08453072812098839
1105	5	-0.1336191468547554
1105	6	-0.0612018691980154
1105	7	-0.041646403469755455
1105	8	-0.10948384569780956
1105	9	-0.0557864020472536
1105	10-11	-0.12955069191135493
1105	12-13	-0.14822036992165977
1105	14-15	-0.1913310612819572
1105	16-17	-0.13087358511496205
1105	18-19	-0.21925932518732338
1105	20-21	-0.12240452550742731
1105	22-23	-0.11584040020055397
1105	24-25	-0.1329725066837355
1105	26-27	-0.09653699724676557
1105	28-29	-0.1376227735149982
1105	30-31	-0.1162284022275415
1105	32-33	-0.18144243647014946
1105	34-35	-0.2065996915788233
1105	36-37	-0.17260173695933645
1105	38-39	-0.13010768605985135
1105	40-41	-0.23747165735483122
1105	42-43	-0.26139849605620213
1105	44-45	-0.1477695028861774
1105	46-47	-0.17348894127787418
1105	48-49	-0.1694948333488142
1105	50-51	-0.1952840040125352
1105	52-53	-0.1770171692668825
1105	54-55	-0.13478422614648622
1105	56-57	-0.13696573962086944
1105	58-59	-0.15425194211010762
1105	60-61	-0.1334207578512192
1105	62-63	-0.13289118917096232
1105	64-65	-0.13662986288957057
1105	66-67	-0.15953300079927857
1105	68-69	-0.10170770587563993
1105	70-71	-0.11601824761190471
1105	72-73	-0.07412229633754919
1105	74-75	-0.10255201526365454
1105	76-77	-0.15946272086290492
1105	78-79	-0.08460158765459624
1105	80-81	-0.0010380541392720488
1105	82-83	-0.018312253389687783
1105	84-85	-0.042492132057319054
1105	86-87	-0.05750825437980467
1105	88-89	-0.009829187662226957
1105	90-91	0.007126305319268056
1105	92-93	-0.023952040314043188
1105	94-95	-0.023071326709874995
1105	96-97	-0.0853141448946495
1105	98-99	-0.07175684552787942
1105	100	-0.07728124867853481
1106	1	-0.1953186541394203
1106	2	-0.1926417310917259
1106	3	-0.1689636376549899
1106	4	-0.07099134524762007
1106	5	-0.11012674717132853
1106	6	-0.10827554915626791
1106	7	-0.09413483873277784
1106	8	-0.08223995276814122
1106	9	-0.1095689773473012
1106	10-11	-0.14367844096310023
1106	12-13	-0.16834526545980566
1106	14-15	-0.21070839821986453
1106	16-17	-0.21711185993824955
1106	18-19	-0.1777817024554622
1106	20-21	-0.14173243292676574
1106	22-23	-0.1540969766199609
1106	24-25	-0.1316978608280337
1106	26-27	-0.19222968847784472
1106	28-29	-0.1698733516009625
1106	30-31	-0.19100433984937837
1106	32-33	-0.24447871254638187
1106	34-35	-0.26825361091515987
1106	36-37	-0.2795641773652804
1106	38-39	-0.18106770910684844
1106	40-41	-0.23441261996100593
1106	42-43	-0.31224722724452647
1106	44-45	-0.27181335252226546
1106	46-47	-0.33813681681446894
1106	48-49	-0.2007524957289064
1106	50-51	-0.22687373136357536
1106	52-53	-0.2002917330418228
1106	54-55	-0.2578803246144261
1106	56-57	-0.19606054974270393
1106	58-59	-0.13309767735056255
1106	60-61	-0.1855346996310132
1106	62-63	-0.20353699578948437
1106	64-65	-0.21415606938625587
1106	66-67	-0.22228398586903353
1106	68-69	-0.2917098866488601
1106	70-71	-0.3091084958136676
1106	72-73	-0.28573445105012496
1106	74-75	-0.26369363395264145
1106	76-77	-0.281881311029224
1106	78-79	-0.24424124907949363
1106	80-81	-0.2698680451626956
1106	82-83	-0.2540517065145238
1106	84-85	-0.2305293033771747
1106	86-87	-0.24563525182927748
1106	88-89	-0.24709490039484905
1106	90-91	-0.32756951503973397
1106	92-93	-0.2766636628294421
1106	94-95	-0.2784870476706871
1106	96-97	-0.27201605532441775
1106	98-99	-0.26900683818486826
1106	100	-0.19276940591309
1107	1	-0.22439706424128048
1107	2	-0.22106748405529686
1107	3	-0.14619136873997007
1107	4	-0.1863337621320511
1107	5	-0.2298983615282637
1107	6	-0.1953893567199927
1107	7	-0.14921339837550107
1107	8	-0.15641340685782978
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2216	26-27	-0.019757723745712497
2216	28-29	-0.015266405707919262
2216	30-31	-0.09442475615054491
2216	32-33	-0.04983392191451941
2216	34-35	0.01965591109537712
2216	36-37	-0.002048788858580508
2216	38-39	0.058122925291876015
2216	40-41	0.07892399330994238
2216	42-43	0.08667471221990297
2216	44-45	0.03392125968117199
2216	46-47	0.06700914966277338
2216	48-49	0.0965088187647325
2216	50-51	0.10434587993476896
2216	52-53	0.10599484347024202
2216	54-55	0.1464171984796323
2216	56-57	0.11157152853784424
2216	58-59	0.10146165371259741
2216	60-61	0.0503941162112298
2216	62-63	0.059277517347005926
2216	64-65	0.11016586021381869
2216	66-67	0.036245186122535245
2216	68-69	0.07816643202937712
2216	70-71	0.05662825194290377
2216	72-73	0.05369790498144056
2216	74-75	0.07554624470043336
2216	76-77	0.03808568473298379
2216	78-79	0.07658451922254272
2216	80-81	0.057190142591935
2216	82-83	0.10109374996060438
2216	84-85	0.12029856561709806
2216	86-87	0.06663342347962953
2216	88-89	0.11247511488972606
2216	90-91	0.05414688995926298
2216	92-93	0.054717051034067765
2216	94-95	0.04037660048931713
2216	96-97	0.01019767920024961
2216	98-99	0.029542522143373162
2216	100	0.09393873834287803
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	68.0
3	314.0
4	91.0
5	141.0
6	305.0
7	778.0
8	1416.0
9	2257.0
10	3256.0
11	4800.0
12	7278.0
13	10607.0
14	12343.0
15	15478.0
16	20461.0
17	30546.0
18	35933.0
19	18694.0
20	10868.0
21	9432.0
22	8866.0
23	9070.0
24	9573.0
25	11375.0
26	14066.0
27	17639.0
28	22148.0
29	27503.0
30	34809.0
31	44948.0
32	56199.0
33	73564.0
34	103606.0
35	176236.0
36	273364.0
37	554924.0
38	1035189.0
39	696313.0
40	7560.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.81973671881623	20.929113972999623	14.81796237437924	22.433186933804905
2	14.435146070142938	22.02337503026582	40.70840762216832	22.833071277422917
3	16.23528473943675	28.517043200060915	32.19234107459103	23.055330985911304
4	10.908150997406915	18.768935799867815	39.99571090934076	30.32720229338451
5	11.097340008135696	38.82378777675577	35.925438454229564	14.153433760878965
6	26.919606022335397	40.315161905736375	18.63238090932291	14.132851162605316
7	23.543568178397617	35.31935284106153	23.98797388949137	17.14910509104948
8	24.414265479839788	35.03375056290597	21.549527694378792	19.00245626287545
9	25.382806030667314	15.838621826903857	22.70747670445352	36.07109543797531
10-11	22.396712331700783	27.550030963546302	29.943310832958066	20.109945871794856
12-13	24.29379085623439	25.98603368364382	30.03058431432912	19.68959114579267
14-15	21.5808779132057	26.92725024077801	29.085343980906703	22.406527865109584
16-17	18.963283361362134	30.16215261191344	29.59270295399965	21.281861072724777
18-19	18.798414046338284	27.53994256420629	34.10673361064719	19.554909778808245
20-21	20.44482219381666	26.664614327557786	33.949362250493934	18.94120122813162
22-23	19.914708887378552	26.677513336496116	34.17405930669554	19.233718469429792
24-25	19.610822792039063	26.83584789602429	34.07929944414303	19.47402986779361
26-27	19.417547922589772	26.794785441627862	34.10111300961136	19.68655362617101
28-29	19.323248111623535	26.804371782993236	34.25578113125611	19.616598974127122
30-31	19.686598242331485	27.20492693285759	33.82337741062974	19.285097414181188
32-33	19.313147573419663	26.950922285538198	34.2048748741067	19.53105526693544
34-35	19.583710899837463	26.775179763297963	33.8957531260127	19.745356210851874
36-37	19.8387972794587	27.078969582787277	33.3873838555755	19.694849282178527
38-39	19.79156333595629	27.144297832780413	33.435202851414516	19.628935979848784
40-41	19.689870324713198	27.017236399740273	33.42742874581389	19.86546452973263
42-43	19.693584039374198	27.1025700933537	33.185053473402796	20.0187923938693
44-45	20.05999983938175	27.25476047208678	32.67525458736131	20.009985101170162
46-47	20.20620105234666	27.641946335674195	32.00838189059521	20.143470721383935
48-49	20.30251852389449	27.572159979345685	31.589415733271387	20.535905763488437
50-51	20.327976955414545	27.70714613856109	31.30208481279546	20.662792093228905
52-53	20.36372775965443	27.87018412444406	31.131865999106186	20.63422211679532
54-55	20.456834416820545	28.17787056690335	30.998005939350097	20.367289076926006
56-57	20.33732163835055	27.752026388221495	31.052190028151777	20.858461945276176
58-59	20.217745583949064	27.49300490155107	31.41461756212156	20.8746319523783
60-61	20.39584726672107	27.780992650607374	31.079095241090922	20.744064841580634
62-63	20.247080883385244	27.70580456255488	31.310633984299706	20.736480569760165
64-65	20.242500965209047	27.48568843349397	31.453515591769815	20.818295009527162
66-67	20.2096309536858	27.60969761036834	31.20652610861351	20.974145327332348
68-69	20.714361774272994	27.706953701478405	30.916166767003528	20.662517757245073
70-71	20.645756940495087	27.535300332420388	30.986050598332902	20.832892128751627
72-73	20.46852889452374	27.699763162689013	31.012511107558343	20.819196835228897
74-75	20.563474541548587	27.825526342598412	30.766753990745084	20.844245125107914
76-77	20.62164382131473	27.8132189288515	30.63214504387952	20.932992205954243
78-79	20.726968362036825	27.821577075121738	30.75321918762685	20.698235375214587
80-81	20.570233870485595	27.786051649782316	30.768229759228838	20.87548472050325
82-83	20.437656697421424	27.82965538390701	30.94725924330743	20.78542867536414
84-85	20.39152249062903	27.78135371498564	30.715311046056815	21.111812748328514
86-87	20.52675585284281	27.87305961172603	30.573065203708794	21.027119331722368
88-89	20.366002072293508	27.692200612407795	30.718873471862008	21.222923843436696
90-91	20.26010271627177	27.69780422515961	30.657355287264544	21.38473777130408
92-93	20.24689922215457	27.87994809009812	30.616267977878504	21.2568847098688
94-95	20.076526082312533	27.693694004534812	30.758306610576884	21.471473302575767
96-97	20.073033625985385	27.796650399626653	30.62680007483612	21.503515899551847
98-99	20.067156334640686	27.71843655246302	30.587200022843504	21.62720709005278
100	20.145323736299495	27.788487307150394	30.44305922853911	21.623129728011005
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3907.0
1	2288.5
2	4800.0
3	7579.5
4	3806.0
5	1831.5
6	3994.5
7	10028.5
8	17266.0
9	18317.5
10	11029.0
11	4606.0
12	3585.0
13	3799.0
14	4217.5
15	4628.0
16	5084.5
17	5700.5
18	6503.0
19	7390.0
20	8689.0
21	10571.0
22	13448.0
23	17829.0
24	22968.0
25	28609.5
26	36449.0
27	45935.5
28	57198.5
29	68322.0
30	76122.0
31	83859.0
32	92480.5
33	99932.5
34	106279.0
35	111557.0
36	114419.5
37	116150.0
38	116859.5
39	115851.5
40	114589.5
41	112554.0
42	108473.5
43	105205.5
44	102225.0
45	98070.0
46	95295.5
47	92946.5
48	90254.0
49	87856.0
50	85941.5
51	82345.5
52	76885.5
53	72718.5
54	69679.5
55	66302.5
56	61153.5
57	55268.0
58	51474.0
59	48176.5
60	42270.5
61	35289.0
62	30008.0
63	24419.5
64	18634.5
65	14671.5
66	11477.5
67	8618.5
68	6478.0
69	4950.0
70	3788.5
71	2866.5
72	2282.0
73	1812.0
74	1495.0
75	1169.5
76	789.0
77	600.0
78	462.0
79	289.0
80	146.0
81	60.5
82	31.5
83	18.0
84	11.0
85	7.0
86	7.0
87	4.0
88	1.0
89	3.0
90	2.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0063354806547734135
2	0.004164165688583464
3	9.51809300247649E-4
4	0.0
5	0.045181197721130585
6	0.0
7	0.0
8	0.0
9	5.948808126547806E-5
10-11	0.0
12-13	1.4872020316369515E-5
14-15	0.0
16-17	0.0
18-19	8.923212189821708E-5
20-21	1.4872020316369515E-5
22-23	8.923212189821708E-5
24-25	5.948808126547806E-5
26-27	2.2308030474554272E-4
28-29	8.923212189821708E-5
30-31	2.2308030474554272E-4
32-33	0.0013087377878405171
34-35	8.179611174003233E-4
36-37	0.0011897616253095612
38-39	0.0011154015237277136
40-41	1.4872020316369515E-4
42-43	5.80008792338411E-4
44-45	2.6769636569465126E-4
46-47	2.3795232506191224E-4
48-49	2.6769636569465126E-4
50-51	4.0154454854197686E-4
52-53	7.584730361348452E-4
54-55	0.001011297381513127
56-57	0.001888746580178928
58-59	6.246248532875196E-4
60-61	0.0011748896049931915
62-63	0.0012492497065750392
64-65	0.0015466901129024295
66-67	4.312885891747159E-4
68-69	2.974404063273903E-4
70-71	2.974404063273903E-4
72-73	1.6359222348006464E-4
74-75	6.0975283297115E-4
76-77	8.477051580330623E-4
78-79	8.477051580330623E-4
80-81	8.625771783494318E-4
82-83	0.0013384818284732562
84-85	0.0011897616253095612
86-87	0.0018441305192298195
88-89	0.0012641217268914088
90-91	0.0011451455643604527
92-93	0.001160017584676822
94-95	8.030890970839537E-4
96-97	1.4872020316369515E-4
98-99	3.569284875928683E-4
100	2.082082844291732E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3362018.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.37402341866994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.72161353033692	47.230616956407104
2	15.713557197130251	19.60235569208824
3	4.492317765757381	8.406118005763737
4	1.6886711920309734	4.213168659126929
5	0.7785036436675133	2.4279202250818677
6	0.41737187890382416	1.5619898009424844
7	0.2614791958098412	1.1416656638056613
8	0.16954467331703185	0.8460146739189831
9	0.12543504780398637	0.7041499748323047
>10	0.5736215276568205	6.278765471101452
>50	0.03652066915392188	1.5711567190023232
>100	0.019457308120037313	2.204571846355605
>500	0.0010485036748361623	0.4303577307986593
>1k	5.719110910735972E-4	0.6777755199724108
>5k	9.531851517893284E-5	0.3258673355235775
>10k+	1.9063703035786567E-4	2.377505725278661
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29621	0.8810482275823628	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23891	0.7106148747567681	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16058	0.47762980448052333	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10408	0.3095759749055478	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5820	0.17311031648254116	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5142	0.15294385693354406	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4691	0.13952929460817878	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4676	0.13908313399868769	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	8.923212189821708E-5	0.0
4	0.0	0.0	0.0	1.4872020316369515E-4	0.0
5	0.0	0.0	0.0	1.4872020316369515E-4	0.0
6	0.0	0.0	0.0	1.4872020316369515E-4	0.0
7	0.0	0.0	0.0	1.4872020316369515E-4	0.0
8	0.0	0.0	0.0	1.4872020316369515E-4	0.0
9	0.0	0.0	0.0	2.082082844291732E-4	0.0
10-11	0.0	0.0	0.0	2.2308030474554272E-4	0.0
12-13	0.0	0.0	0.0	2.8256838601102075E-4	0.0
14-15	0.0	0.0	0.0	3.718005079092378E-4	0.0
16-17	0.0	0.0	0.0	4.015445485419769E-4	0.0
18-19	0.0	0.0	0.0	4.461606094910854E-4	0.0
20-21	0.0	0.0	0.0	4.461606094910854E-4	0.0
22-23	0.0	0.0	0.0	6.0975283297115E-4	0.0
24-25	0.0	0.0	0.0	9.81553340880388E-4	0.0
26-27	0.0	0.0	0.0	0.001502074051953321	0.0
28-29	0.0	0.0	0.0	0.0032569724492849234	0.0
30-31	0.0	0.0	0.0	0.00675189722363176	0.0
32-33	0.0	0.0	0.0	0.013697130711376323	0.0
34-35	0.0	0.0	0.0	0.024375241298529634	0.0
36-37	0.0	0.0	0.0	0.040600615463688774	0.0
38-39	0.0	0.0	0.0	0.06384558321817432	0.0
40-41	0.0	0.0	0.0	0.08865211310587867	0.0
42-43	0.0	0.0	0.0	0.11396429168433958	0.0
44-45	0.0	0.0	0.0	0.1446452695970099	0.0
46-47	0.0	0.0	0.0	0.1804570945188277	0.0
48-49	0.0	0.0	0.0	0.22315466484712454	0.0
50-51	0.0	0.0	0.0	0.2661199315411161	0.0
52-53	0.0	0.0	0.0	0.3084605733818201	0.0
54-55	0.0	0.0	0.0	0.3549951249517403	0.0
56-57	0.0	0.0	0.0	0.41388832540456355	0.0
58-59	0.0	0.0	0.0	0.4837422048305512	0.0
60-61	0.0	0.0	0.0	0.5601992612770068	0.0
62-63	0.0	0.0	0.0	0.63494603538708	0.0
64-65	0.0	0.0	0.0	0.7181549890571675	0.0
66-67	0.0	0.0	0.0	0.8168457158765956	0.0
68-69	0.0	0.0	0.0	0.9278356034976613	0.0
70-71	0.0	0.0	0.0	1.0547385528572422	0.0
72-73	0.0	0.0	0.0	1.1886164797452006	0.0
74-75	0.0	0.0	0.0	1.3260785635294041	0.0
76-77	0.0	0.0	0.0	1.4767023852935945	0.0
78-79	0.0	0.0	0.0	1.6509132312795471	0.0
80-81	0.0	0.0	0.0	1.8505254879658586	0.0
82-83	0.0	0.0	0.0	2.0640728276886087	0.0
84-85	0.0	0.0	0.0	2.2804458512714687	0.0
86-87	0.0	0.0	0.0	2.5132078412429677	0.0
88	0.0	0.0	0.0	2.705488191913309	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9525	0.0	66.79474	1
GTATCAA	19190	0.0	46.3613	1
GTGGTAT	2755	0.0	37.720737	1
TGGTATC	2860	0.0	36.005447	2
ATCAACG	25130	0.0	35.211533	3
TATCAAC	25445	0.0	34.79669	2
TCAACGC	25415	0.0	34.77916	4
CAACGCA	26215	0.0	33.73574	5
AACGCAG	26990	0.0	32.928505	6
CGCAGAG	30220	0.0	29.25349	8
ACGCAGA	30380	0.0	29.192244	7
GCAGAGT	32365	0.0	27.067844	9
GTACATG	14665	0.0	25.042543	1
TATAACG	320	0.0	24.979805	2
TACATGG	14745	0.0	24.331554	2
CATGGGT	7280	0.0	23.96039	4
ACATGGG	14740	0.0	23.763836	3
GAGTACT	24335	0.0	23.156408	12-13
CAGAGTA	31990	0.0	23.109888	10-11
ATAACGC	310	0.0	22.750309	3
>>END_MODULE