FastQCFastQC Report
Wed 25 May 2016
SRR2431402_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431402_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4743158
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA256710.5412216923830072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58950.048.767991
GTATCAA104600.038.595181
TCAACGC117900.032.877184
ATCAACG119050.032.52423
CAACGCA125800.030.7745115
TATCAAC130550.030.2724652
AACGCAG133500.028.9704386
GTACATG217600.027.223521
TACATGG220750.026.2149942
CGCAGAG146600.025.6764558
ACGCAGA149950.025.2908577
CATGGGT106650.025.0094054
ACATGGG224750.024.617493
GCAGAGT165250.021.8119749
ATGGGTG61250.019.8146135
TACCTGG35500.019.6145672
ATGGGTA46200.018.7347495
CAGAGTA165350.018.57321410-11
AGAGTAC149700.018.33313210-11
GTACCTG40100.018.3044931
Produced by FastQC (version 0.11.5)