##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431402_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4743158
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.76910594165322	31.0	31.0	34.0	27.0	34.0
2	30.903007658610573	33.0	31.0	34.0	27.0	34.0
3	31.0115800485668	34.0	31.0	34.0	27.0	34.0
4	34.23493356114218	37.0	35.0	37.0	30.0	37.0
5	34.31705774928855	37.0	35.0	37.0	31.0	37.0
6	34.3642149386548	37.0	35.0	37.0	31.0	37.0
7	34.258692204645094	37.0	35.0	37.0	31.0	37.0
8	34.24835394477687	37.0	35.0	37.0	30.0	37.0
9	35.71957206569969	39.0	37.0	39.0	30.0	39.0
10-11	35.72528439912817	39.0	37.0	39.0	30.0	39.0
12-13	35.65255806363608	39.0	37.0	39.0	30.0	39.0
14-15	37.01560226330221	41.0	38.0	41.0	30.0	41.0
16-17	37.048773939219394	41.0	38.0	41.0	30.0	41.0
18-19	37.08765847564007	41.0	38.0	41.0	30.0	41.0
20-21	37.178233784326814	41.0	38.0	41.0	30.0	41.0
22-23	37.15371847195476	40.0	38.0	41.0	30.0	41.0
24-25	37.202895728963696	40.5	38.0	41.0	30.0	41.0
26-27	37.23648073709541	40.0	38.0	41.0	30.0	41.0
28-29	37.19729734915008	40.0	38.0	41.0	30.0	41.0
30-31	37.127607492729524	40.0	38.0	41.0	30.0	41.0
32-33	37.07073230535437	40.0	38.0	41.0	30.0	41.0
34-35	36.994220938876595	40.0	38.0	41.0	30.0	41.0
36-37	36.86276790695145	40.0	38.0	41.0	30.0	41.0
38-39	36.74046299954587	40.0	37.0	41.0	30.0	41.0
40-41	36.67858840038641	40.0	37.0	41.0	29.5	41.0
42-43	36.520269301591895	40.0	37.0	41.0	29.0	41.0
44-45	36.380265426536496	40.0	37.0	41.0	29.0	41.0
46-47	36.256173734882964	40.0	36.0	41.0	28.5	41.0
48-49	36.124747900027785	40.0	36.0	41.0	28.0	41.0
50-51	35.99966688860038	40.0	36.0	41.0	28.0	41.0
52-53	35.84690864187952	40.0	35.0	41.0	28.0	41.0
54-55	35.693398259134526	40.0	35.0	41.0	28.0	41.0
56-57	35.511545999521836	39.0	35.0	41.0	27.5	41.0
58-59	35.307777539774136	39.0	35.0	41.0	27.0	41.0
60-61	35.08218553967631	39.0	34.5	41.0	26.5	41.0
62-63	34.99439656026639	39.0	34.5	41.0	26.5	41.0
64-65	34.75735206375162	38.0	34.0	40.5	26.0	41.0
66-67	34.51498115812292	38.0	34.0	40.0	26.0	41.0
68-69	34.23196254478556	37.0	34.0	40.0	26.0	41.0
70-71	33.909649541508	37.0	34.0	40.0	25.5	41.0
72-73	33.575924310343446	36.0	33.0	39.0	25.0	41.0
74-75	33.29030732267405	36.0	33.0	39.0	25.0	41.0
76-77	33.01512958244275	35.5	33.0	39.0	24.0	40.5
78-79	32.65726663543572	35.0	33.0	37.5	23.5	39.5
80-81	32.31262620810861	35.0	33.0	37.0	23.0	39.0
82-83	31.967363726867205	35.0	33.0	37.0	22.0	39.0
84-85	31.650267184858695	35.0	32.0	36.0	20.5	38.0
86-87	31.350830079875053	35.0	32.0	36.0	20.0	37.0
88-89	31.068539146281864	35.0	32.0	36.0	19.0	37.0
90-91	30.824449132835127	35.0	32.0	35.0	18.0	36.5
92-93	30.584660789288485	35.0	32.0	35.0	12.5	36.0
94-95	30.369259678889044	35.0	31.5	35.0	4.5	36.0
96-97	30.166156704035583	35.0	31.0	35.0	2.0	36.0
98-99	29.950712373486187	35.0	31.0	35.0	2.0	35.5
100	29.78427010021593	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2654944842195093
1101	2	0.23367723711530886
1101	3	0.26847592745328797
1101	4	0.2937957190515945
1101	5	0.24540583817472594
1101	6	0.24432854042771623
1101	7	0.2687400763964831
1101	8	0.22502857323468817
1101	9	0.21816656493238185
1101	10-11	0.21965161136693467
1101	12-13	0.1263962064665023
1101	14-15	0.2006473989726416
1101	16-17	0.19981415262190438
1101	18-19	0.08535983452835438
1101	20-21	0.1442079690299849
1101	22-23	0.1846890567532924
1101	24-25	0.13046664024704313
1101	26-27	0.10240824656147396
1101	28-29	0.16016262590196817
1101	30-31	0.20839542905321196
1101	32-33	0.19429866641934979
1101	34-35	0.1835666627918755
1101	36-37	0.0918031444572378
1101	38-39	0.21895496331821818
1101	40-41	0.14201670454335868
1101	42-43	0.0801981162359624
1101	44-45	0.04862157780956977
1101	46-47	0.12655556484025965
1101	48-49	0.1750863303665966
1101	50-51	0.12563171806285567
1101	52-53	0.07244286300548453
1101	54-55	0.08665947396438156
1101	56-57	0.09362917099592494
1101	58-59	0.022241166360018383
1101	60-61	0.0612259763534766
1101	62-63	0.05582146735647342
1101	64-65	0.07613321053405286
1101	66-67	0.06275135166995938
1101	68-69	0.06074811563131277
1101	70-71	0.0496799693136154
1101	72-73	-0.012038223981704732
1101	74-75	0.0851280903121534
1101	76-77	0.15321686718740324
1101	78-79	0.12331645167390093
1101	80-81	0.05806817756203486
1101	82-83	0.07489941302640091
1101	84-85	-0.04284655126118153
1101	86-87	0.03847061962013498
1101	88-89	3.669289562679978E-4
1101	90-91	-0.1081899615273123
1101	92-93	-0.10646494482645963
1101	94-95	-0.06490241994701407
1101	96-97	-0.08773748119409319
1101	98-99	-0.13625654336534154
1101	100	-0.14709616279487392
1102	1	-0.07913066771627442
1102	2	-0.03587004211106759
1102	3	-0.026061748290228337
1102	4	0.033501649280538004
1102	5	0.02560249049986396
1102	6	0.06897107523376178
1102	7	-0.09418028222769692
1102	8	-0.012684020827592235
1102	9	5.12403216610835E-4
1102	10-11	0.06632965905092902
1102	12-13	-0.05389593217061872
1102	14-15	0.007000245567546415
1102	16-17	-0.04799758793514286
1102	18-19	-0.00949366244740446
1102	20-21	-0.05743762312349787
1102	22-23	-0.04069626953350536
1102	24-25	-0.06537257002398889
1102	26-27	-0.03298759303519461
1102	28-29	-0.008950765548071615
1102	30-31	-0.00654193189949126
1102	32-33	0.015026591100145481
1102	34-35	0.008864497341399158
1102	36-37	0.03735306215974532
1102	38-39	0.05493903483874618
1102	40-41	-0.041271445378370686
1102	42-43	-0.023305110840738053
1102	44-45	-0.037723582001753186
1102	46-47	-0.0864268228007603
1102	48-49	-0.07227781393920907
1102	50-51	-0.16305993603197066
1102	52-53	-0.17990992922631932
1102	54-55	-0.19767190577618265
1102	56-57	-0.1898570600590901
1102	58-59	-0.19996169739435743
1102	60-61	-0.2612993285851388
1102	62-63	-0.1904484417438752
1102	64-65	-0.12908449084006435
1102	66-67	-0.14416363122454356
1102	68-69	-0.19149603921312064
1102	70-71	-0.20912966338497085
1102	72-73	-0.22322912736376566
1102	74-75	-0.19423878067659928
1102	76-77	-0.1525055849849437
1102	78-79	-0.15345918889250498
1102	80-81	-0.23801219571709709
1102	82-83	-0.2422875368608075
1102	84-85	-0.2780308388661865
1102	86-87	-0.2614336808792288
1102	88-89	-0.29624541664795245
1102	90-91	-0.3107722069359724
1102	92-93	-0.25310428766531246
1102	94-95	-0.22472218492517015
1102	96-97	-0.25814040864745635
1102	98-99	-0.2919768902996829
1102	100	-0.37211905725818184
1103	1	-0.3177829050984293
1103	2	-0.2652303411566663
1103	3	-0.22744933469317985
1103	4	-0.107700998809662
1103	5	-0.21921566040339258
1103	6	-0.1324447107523099
1103	7	-0.2165620805124817
1103	8	-0.1268972959438628
1103	9	-0.08417199908443962
1103	10-11	-0.14683246123092175
1103	12-13	-0.15370337118550026
1103	14-15	-0.17474551858713028
1103	16-17	-0.1908825036794397
1103	18-19	-0.2233898284197835
1103	20-21	-0.30268746484125586
1103	22-23	-0.24027640321710209
1103	24-25	-0.24194131678620323
1103	26-27	-0.22931245381402476
1103	28-29	-0.23121790309799906
1103	30-31	-0.23352984218475115
1103	32-33	-0.26583763300238417
1103	34-35	-0.26515954890872706
1103	36-37	-0.3354412318961906
1103	38-39	-0.24925538081801335
1103	40-41	-0.15304662055620355
1103	42-43	-0.33235079917279364
1103	44-45	-0.40379398854222615
1103	46-47	-0.4511341177166628
1103	48-49	-0.3682713779334179
1103	50-51	-0.4903762967888028
1103	52-53	-0.5979115811420783
1103	54-55	-0.5913582694856245
1103	56-57	-0.5468586431889619
1103	58-59	-0.634482133614668
1103	60-61	-0.5537053301943615
1103	62-63	-0.5043577675217179
1103	64-65	-0.5667634999928097
1103	66-67	-0.5472133326823183
1103	68-69	-0.4989461011336047
1103	70-71	-0.5384051923753361
1103	72-73	-0.5421858051612105
1103	74-75	-0.5127970405321562
1103	76-77	-0.47517916110584935
1103	78-79	-0.46789464334109
1103	80-81	-0.46188517214444147
1103	82-83	-0.4979035585110587
1103	84-85	-0.5632404122820454
1103	86-87	-0.5409580953491471
1103	88-89	-0.5870328192880123
1103	90-91	-0.5634552742810612
1103	92-93	-0.5629470754286601
1103	94-95	-0.6112078158319854
1103	96-97	-0.5843236469030977
1103	98-99	-0.6737415068859391
1103	100	-0.5884425396835624
1104	1	-0.17270581679697372
1104	2	-0.1037099004460238
1104	3	-0.07553096077106503
1104	4	0.019746511942081213
1104	5	-0.011483543298616894
1104	6	0.02730855579384439
1104	7	-0.027976545788582996
1104	8	-0.04078280499537357
1104	9	0.024456506912727605
1104	10-11	-0.03866639564258634
1104	12-13	-0.05603629423912082
1104	14-15	-0.12408032948331282
1104	16-17	-0.10696093250685124
1104	18-19	-0.1312276695218344
1104	20-21	-0.11078541886830351
1104	22-23	-0.12212791287867475
1104	24-25	-0.12990183864273774
1104	26-27	-0.1349917812137562
1104	28-29	-0.10373138733466902
1104	30-31	-0.13084197137656872
1104	32-33	-0.23289437628978504
1104	34-35	-0.2091212851427855
1104	36-37	-0.30585168625363934
1104	38-39	-0.2162278281506076
1104	40-41	-0.29030481581001055
1104	42-43	-0.3564894642884866
1104	44-45	-0.32528320762150287
1104	46-47	-0.41276277100878644
1104	48-49	-0.3682285476369387
1104	50-51	-0.4249912180388691
1104	52-53	-0.5203069414411488
1104	54-55	-0.40935464242786423
1104	56-57	-0.4459190135341302
1104	58-59	-0.5898080175853551
1104	60-61	-0.5607736400257579
1104	62-63	-0.44228926449194006
1104	64-65	-0.41848962326300665
1104	66-67	-0.34060039078079285
1104	68-69	-0.3347702142767659
1104	70-71	-0.40426185353516075
1104	72-73	-0.4419720125679021
1104	74-75	-0.3440740504281692
1104	76-77	-0.3386339839111301
1104	78-79	-0.3496809404915382
1104	80-81	-0.33330128315266094
1104	82-83	-0.41290833947245176
1104	84-85	-0.39564189475615663
1104	86-87	-0.33537482128265594
1104	88-89	-0.3970646589822735
1104	90-91	-0.4663081137234073
1104	92-93	-0.5293105825375228
1104	94-95	-0.5030537633574141
1104	96-97	-0.5409642361585796
1104	98-99	-0.536186164361979
1104	100	-0.49726395178876714
1105	1	-0.08410280437425754
1105	2	-0.0014041783025220411
1105	3	0.02012950108458611
1105	4	0.12476402258096186
1105	5	0.05469044711811222
1105	6	0.11137746647831648
1105	7	0.06237670597487721
1105	8	0.039280707990215546
1105	9	0.06444986909450989
1105	10-11	-0.012860608782688132
1105	12-13	0.04566073219336886
1105	14-15	-0.0165130116341885
1105	16-17	-0.017089692141851742
1105	18-19	0.01659299665582381
1105	20-21	0.053371265524162936
1105	22-23	-0.027035976474699908
1105	24-25	-0.055623878968852125
1105	26-27	-0.010151981265032362
1105	28-29	-0.024208904315379698
1105	30-31	0.02593673662695295
1105	32-33	0.05881528136361425
1105	34-35	-0.01570259649201944
1105	36-37	-0.041124708918083286
1105	38-39	-0.05316514905732106
1105	40-41	-0.0926364202858494
1105	42-43	-0.08270998159093779
1105	44-45	-0.052747311583480894
1105	46-47	-0.11255012656974372
1105	48-49	-0.1859422640933488
1105	50-51	-0.15729505598578442
1105	52-53	-0.14767848255454652
1105	54-55	-0.24947771860543355
1105	56-57	-0.2860995860381337
1105	58-59	-0.20409951971076623
1105	60-61	-0.29726237330399385
1105	62-63	-0.18648770650322888
1105	64-65	-0.24445477611273958
1105	66-67	-0.26884583165720954
1105	68-69	-0.17836188663527253
1105	70-71	-0.154060336257956
1105	72-73	-0.09334174229859116
1105	74-75	-0.11996827528530218
1105	76-77	-0.13949340123856047
1105	78-79	-0.1328539106259683
1105	80-81	-0.13109611139668687
1105	82-83	-0.05530787058827258
1105	84-85	-0.13642472304432474
1105	86-87	-0.15454309225786744
1105	88-89	-0.14646598469385452
1105	90-91	-0.21025560789628628
1105	92-93	-0.23558634409480916
1105	94-95	-0.23242871369957285
1105	96-97	-0.20223886301175398
1105	98-99	-0.23255082354883072
1105	100	-0.1973249091001712
1106	1	-0.15487536424880588
1106	2	-0.18130892474139415
1106	3	-0.13751866148297154
1106	4	-0.07839933652524422
1106	5	-0.06968608122481612
1106	6	-0.03231344654194146
1106	7	0.0070144721160687595
1106	8	-0.12910091140686575
1106	9	-0.06330296478220987
1106	10-11	-0.04093462175003282
1106	12-13	-0.02421946849977985
1106	14-15	-0.11717783851295849
1106	16-17	-0.11815686546458437
1106	18-19	-0.15093494286745113
1106	20-21	-0.12565279696494258
1106	22-23	-0.0639859005897776
1106	24-25	-0.07003235988977252
1106	26-27	-0.17051159391187554
1106	28-29	-0.16548731033763886
1106	30-31	-0.14412272785874336
1106	32-33	-0.20690015192912625
1106	34-35	-0.16553694699928911
1106	36-37	-0.23359949552056491
1106	38-39	-0.2134502810743868
1106	40-41	-0.17628406636729466
1106	42-43	-0.25839370315838295
1106	44-45	-0.3178563880473533
1106	46-47	-0.33742474233828545
1106	48-49	-0.2901859781037288
1106	50-51	-0.281155486021305
1106	52-53	-0.3426132731200724
1106	54-55	-0.280886357346084
1106	56-57	-0.30156834944576616
1106	58-59	-0.37227374045640715
1106	60-61	-0.33591265610994014
1106	62-63	-0.3019944266604213
1106	64-65	-0.27169264246497704
1106	66-67	-0.1695701124078326
1106	68-69	-0.16061867788482687
1106	70-71	-0.18013796626105005
1106	72-73	-0.20515878309111457
1106	74-75	-0.221907641480243
1106	76-77	-0.16541531386031494
1106	78-79	-0.17725371431878756
1106	80-81	-0.1691431194716131
1106	82-83	-0.20941686630525425
1106	84-85	-0.14305190701682946
1106	86-87	-0.21009539562185608
1106	88-89	-0.24448304457497372
1106	90-91	-0.2377017914059465
1106	92-93	-0.2377791444611681
1106	94-95	-0.22989814806562592
1106	96-97	-0.26467359295589077
1106	98-99	-0.3405273254714487
1106	100	-0.4163814426128596
1107	1	-0.3685992210096529
1107	2	-0.41603922706873675
1107	3	-0.34003642037065873
1107	4	-0.3240785848621428
1107	5	-0.2760831310665708
1107	6	-0.3528803605386699
1107	7	-0.3165467702111968
1107	8	-0.30916488999005765
1107	9	-0.40456882274729367
1107	10-11	-0.40122287358003206
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2216	54-55	0.3808663500718623
2216	56-57	0.4032375305781315
2216	58-59	0.3433221435861711
2216	60-61	0.3891879467907273
2216	62-63	0.3481937295569466
2216	64-65	0.35581714101573425
2216	66-67	0.27450952860998257
2216	68-69	0.26502422420423954
2216	70-71	0.2482752043281522
2216	72-73	0.30966739793331044
2216	74-75	0.28480051198879863
2216	76-77	0.31613042419004955
2216	78-79	0.31267774076420096
2216	80-81	0.3328923931916705
2216	82-83	0.35378980697517903
2216	84-85	0.30853527334869213
2216	86-87	0.26556876147140684
2216	88-89	0.2942244188478824
2216	90-91	0.30796720490803864
2216	92-93	0.2768364143007638
2216	94-95	0.2830959010275045
2216	96-97	0.2909696848030201
2216	98-99	0.2552805451083131
2216	100	0.3034880813195322
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	108745.0
3	21020.0
4	14582.0
5	12502.0
6	8479.0
7	7529.0
8	8375.0
9	9242.0
10	9494.0
11	10204.0
12	11588.0
13	12970.0
14	13872.0
15	14191.0
16	14056.0
17	14262.0
18	14069.0
19	13684.0
20	13426.0
21	14126.0
22	16709.0
23	23877.0
24	37168.0
25	49805.0
26	57608.0
27	47838.0
28	43552.0
29	53246.0
30	64777.0
31	72262.0
32	90543.0
33	120509.0
34	169922.0
35	261671.0
36	431499.0
37	805822.0
38	1320533.0
39	726121.0
40	3280.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.95709464867954	21.75368136396219	14.272489996396887	22.01673399096138
2	14.596103290509626	22.496025403626383	40.79610616269404	22.111765143169944
3	16.792274808831383	29.0759632674071	30.976380909815386	23.155381013946126
4	11.39523243314023	19.439757038676554	40.25768177219842	28.9073287559848
5	11.72388970492252	40.31240640080164	35.34873925972302	12.614964634552821
6	27.07864545058848	40.939502866584256	19.226232183935693	12.755619498891576
7	24.248544266166252	35.47106107900059	23.75005644014791	16.53033821468524
8	23.480519502438145	37.472685025592426	21.682235340629703	17.364560131339722
9	24.48558069085689	18.632799933338184	22.023544699805605	34.858074675999326
10-11	21.716245739476996	29.418258615724298	29.92753814295525	18.937957501843456
12-13	22.78501429710423	27.533123435710287	29.36517397148512	20.31668829570036
14-15	20.693807399330932	29.171574057498777	26.800994414514562	23.333624128655728
16-17	19.81783406756941	32.19075271820651	27.36217831198416	20.629234902239922
18-19	19.865145791768725	31.03177425406808	29.283916538032408	19.819163416130795
20-21	21.730596287909837	30.07959753427504	28.417654185608725	19.772151992206393
22-23	21.222936926732164	30.191472288360256	28.41827207945186	20.167318705455724
24-25	20.841834770252866	30.252463931086936	28.45656592934217	20.449135369318032
26-27	20.560651340596024	30.13222457152629	28.513989752308238	20.793134335569444
28-29	20.501670049876278	30.18181986964296	28.69531038939489	20.62119969108587
30-31	20.847773862266365	30.612036491197276	28.25124473737402	20.28894490916234
32-33	20.40793742755377	30.35468113952675	28.75974582475512	20.47763560816436
34-35	20.617349211672305	29.998332189988563	28.70787342408569	20.676445174253445
36-37	20.919235969304996	30.287257943443073	28.235016086550335	20.558490000701592
38-39	20.726299270746793	30.287655444343343	28.48813809655682	20.497907188353047
40-41	20.590919472386922	30.046221791810467	28.64644469868276	20.716414037119847
42-43	20.4690149767671	30.027370987386348	28.69969084340514	20.80392319244141
44-45	20.751635348878825	30.142255490561404	28.520807301412425	20.585301859147346
46-47	20.7280922308675	30.33973635573461	28.38684283180691	20.545328581590983
48-49	20.64423218438948	30.051849386210673	28.492474743931346	20.811443685468507
50-51	20.611499125727498	30.00379375397864	28.487853551812485	20.896853568481376
52-53	20.648127146225544	30.010402105740674	28.510019675382566	20.83145107265122
54-55	20.798518692164105	30.324251837102135	28.29595688247199	20.58127258826177
56-57	20.64539895629248	30.057280697571677	28.27610186592152	21.02121848021433
58-59	20.58983208061616	29.790466858171822	28.58002387695393	21.03967718425809
60-61	20.832855518046543	30.20419970361562	28.158921698461647	20.804023079876195
62-63	20.712696451613173	30.086971216774224	28.36546941207348	20.834862919539123
64-65	20.68428783496733	29.866525758083394	28.469437831296222	20.979748575653055
66-67	20.512511882308523	29.94369175611738	28.32721253569176	21.21658382588234
68-69	21.01489674917204	30.052581415747632	28.15019855827022	20.782323276810114
70-71	20.90020452576952	29.813346632940853	28.345927770515107	20.940521070774523
72-73	20.68608804354235	29.96401673754918	28.551679224188497	20.798215994719964
74-75	20.717536405526648	29.99677628357396	28.368904976527844	20.916782334371547
76-77	20.72026700123755	30.001781794616367	28.246508384087406	21.031442820058682
78-79	20.9040652396246	29.97356932290312	28.399448705972823	20.72291673149945
80-81	20.680940678718894	29.893073617096068	28.464979642062367	20.96100606212267
82-83	20.622195804974883	29.86609072003457	28.668674124264033	20.843039350726517
84-85	20.60869649619074	29.86400567854522	28.413763213610956	21.113534611653087
86-87	20.842278679562455	29.949937007179557	28.251683905381853	20.95610040787614
88-89	20.69583563322581	29.737071650301562	28.41416927883812	21.152923437634513
90-91	20.590812541315547	29.701057445828503	28.40616790299443	21.301962109861524
92-93	20.702860866899574	29.89224147479555	28.308892019316197	21.09600563898869
94-95	20.56704610461719	29.77419276913471	28.425777841820903	21.232983284427192
96-97	20.534895906021163	29.811553067163892	28.410387572416195	21.24316345439875
98-99	20.548214874050405	29.791924409882103	28.3710755381077	21.288785177959788
100	20.65614713807108	29.904881960743683	28.190427677730963	21.248543223454277
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	23847.0
1	17291.0
2	9158.5
3	6329.0
4	4273.0
5	3048.5
6	2506.0
7	2451.5
8	2687.0
9	2741.0
10	2236.5
11	1749.0
12	1675.0
13	1724.0
14	1821.5
15	1993.5
16	2277.0
17	2815.0
18	3739.5
19	5087.5
20	7152.0
21	10326.0
22	15043.0
23	22107.0
24	30625.5
25	39751.5
26	51354.0
27	65662.5
28	83276.0
29	101441.0
30	114465.5
31	126265.0
32	137997.5
33	148936.0
34	159594.0
35	165843.5
36	168504.0
37	170886.5
38	171457.0
39	169034.5
40	166130.0
41	161298.0
42	154153.5
43	148936.0
44	144719.0
45	139829.5
46	136358.5
47	132736.0
48	128153.0
49	124452.0
50	121405.0
51	116559.5
52	109368.0
53	102730.5
54	98401.5
55	95670.5
56	88922.0
57	79740.0
58	72907.0
59	66268.5
60	58596.0
61	49463.5
62	41136.5
63	33415.0
64	26103.0
65	21033.5
66	17047.0
67	13255.0
68	10150.0
69	7881.0
70	6142.5
71	4851.5
72	3805.5
73	2944.0
74	2490.5
75	2074.5
76	1494.0
77	1170.0
78	943.0
79	695.5
80	480.0
81	323.0
82	259.0
83	206.0
84	158.0
85	139.0
86	127.5
87	107.5
88	89.0
89	86.0
90	84.0
91	68.5
92	56.5
93	49.0
94	40.5
95	34.0
96	32.5
97	32.0
98	25.5
99	25.5
100	35.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17633821179897444
2	0.17047713780565604
3	0.18394917479029793
4	0.17389258380176245
5	0.1871327077866687
6	0.07473923491479727
7	0.07640479191289853
8	0.06501997192587723
9	0.05930647893239062
10-11	0.0721671089177295
12-13	0.07140812091859475
14-15	0.06922603042108233
16-17	0.06501997192587723
18-19	0.0695422754207218
20-21	0.07116566641887113
22-23	0.06918386442113039
24-25	0.06900465892133469
26-27	0.07287338941692434
28-29	0.07019584841997674
30-31	0.07598313191337923
32-33	0.07244118791741705
34-35	0.07188248841805396
36-37	0.08328839140505123
38-39	0.0784393014105792
40-41	0.07401187141562646
42-43	0.07696349141226162
44-45	0.08822181339942714
46-47	0.07808089041098779
48-49	0.08328839140505123
50-51	0.07988348690893282
52-53	0.07870283891027875
54-55	0.08080059740788732
56-57	0.07412782791549428
58-59	0.08138037990722637
60-61	0.07810197341096375
62-63	0.06986906192034927
64-65	0.07256768591727283
66-67	0.07259931041723679
68-69	0.07095483641911149
70-71	0.0668014854238463
72-73	0.06403961242699484
74-75	0.0692998209209982
76-77	0.07493952341456894
78-79	0.07169274141827028
80-81	0.0786185069103749
82-83	0.08074788990794741
84-85	0.08256102790588044
86-87	0.0786185069103749
88-89	0.08024189790852423
90-91	0.07840767691061525
92-93	0.06959498292066171
94-95	0.06950010942076987
96-97	0.06920494742110636
98-99	0.07805980741101182
100	0.0864192169014821
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4743158.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.66471246391646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.17908638649308	49.26098716946428
2	15.064491336271491	19.482820013503133
3	4.555345572828552	8.837103349221993
4	1.746064774142514	4.516351062531957
5	0.8322628622501669	2.690901934090158
6	0.43312014445003666	1.680455376191495
7	0.269891774707333	1.221673180547807
8	0.18657559616994257	0.9651885823290505
9	0.12414843602369836	0.7225220626485644
>10	0.5585174733402781	6.148901510384964
>50	0.03277933776134766	1.468718806182775
>100	0.01686919031999608	1.9493230489521647
>500	5.86464529224429E-4	0.2739344758115763
>1k	2.2806937336577898E-4	0.24026664756721544
>5k	0.0	0.0
>10k+	3.2581339051865744E-5	0.5408527805726868
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25671	0.5412216923830072	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	4.216599995193076E-5	0.0	0.0	0.0	0.0
10-11	4.216599995193076E-5	0.0	0.0	1.054149998798269E-5	0.0
12-13	4.216599995193076E-5	0.0	0.0	2.108299997596538E-5	0.0
14-15	4.216599995193076E-5	0.0	0.0	2.108299997596538E-5	0.0
16-17	4.216599995193076E-5	0.0	0.0	4.216599995193076E-5	0.0
18-19	9.487349989184421E-5	0.0	0.0	4.216599995193076E-5	0.0
20-21	1.054149998798269E-4	0.0	0.0	8.433199990386152E-5	1.054149998798269E-5
22-23	1.054149998798269E-4	0.0	0.0	2.213714997476365E-4	2.108299997596538E-5
24-25	1.1595649986780959E-4	0.0	0.0	5.481579993750999E-4	2.108299997596538E-5
26-27	1.2649799985579228E-4	0.0	0.0	9.592764989064249E-4	2.108299997596538E-5
28-29	1.2649799985579228E-4	0.0	0.0	0.002066133997644607	2.108299997596538E-5
30-31	1.2649799985579228E-4	0.0	0.0	0.004764757994568176	2.108299997596538E-5
32-33	1.2649799985579228E-4	0.0	0.0	0.010246337988319174	2.108299997596538E-5
34-35	1.2649799985579228E-4	0.0	0.0	0.01850033247890962	2.108299997596538E-5
36-37	1.2649799985579228E-4	0.0	0.0	0.03175099796380386	2.108299997596538E-5
38-39	1.2649799985579228E-4	0.0	0.0	0.05102085994183622	4.216599995193076E-5
40-41	1.2649799985579228E-4	0.0	0.0	0.07353750391616724	4.216599995193076E-5
42-43	1.2649799985579228E-4	0.0	0.0	0.09541111639123133	4.216599995193076E-5
44-45	1.2649799985579228E-4	0.0	0.0	0.12148024586151251	5.270749993991345E-5
46-47	1.2649799985579228E-4	0.0	0.0	0.1554765833227567	6.324899992789614E-5
48-49	1.2649799985579228E-4	0.0	0.0	0.1931624457797948	6.324899992789614E-5
50-51	1.4758099983175766E-4	0.0	0.0	0.23292498373446552	6.324899992789614E-5
52-53	1.5812249981974035E-4	0.0	0.0	0.2717809526901697	6.324899992789614E-5
54-55	1.6866399980772304E-4	0.0	0.0	0.31631879013939657	6.324899992789614E-5
56-57	2.108299997596538E-4	0.0	0.0	0.370839428077243	7.379049991587883E-5
58-59	2.5299599971158456E-4	0.0	0.0	0.4345417125046225	8.433199990386152E-5
60-61	2.5299599971158456E-4	0.0	0.0	0.503588537425909	8.433199990386152E-5
62-63	2.5299599971158456E-4	0.0	0.0	0.5746277058449244	8.433199990386152E-5
64-65	2.5299599971158456E-4	0.0	0.0	0.6492826087598178	8.433199990386152E-5
66-67	2.5299599971158456E-4	0.0	0.0	0.7370300546597857	8.433199990386152E-5
68-69	2.5299599971158456E-4	0.0	0.0	0.8383338695442994	8.433199990386152E-5
70-71	2.6353749969956725E-4	0.0	0.0	0.95480690291152	8.433199990386152E-5
72-73	2.7407899968754995E-4	0.0	0.0	1.0825909657658463	8.433199990386152E-5
74-75	2.7407899968754995E-4	0.0	0.0	1.2093630446213262	8.433199990386152E-5
76-77	2.7407899968754995E-4	0.0	0.0	1.3486900499624932	8.433199990386152E-5
78-79	2.7407899968754995E-4	0.0	0.0	1.512800121775408	8.433199990386152E-5
80-81	2.7407899968754995E-4	0.0	0.0	1.6976874900646362	8.433199990386152E-5
82-83	2.7407899968754995E-4	0.0	0.0	1.8971432113372568	8.433199990386152E-5
84-85	2.7407899968754995E-4	0.0	0.0	2.0974844186088677	8.433199990386152E-5
86-87	2.7407899968754995E-4	0.0	0.0	2.3126680578635583	8.433199990386152E-5
88	2.7407899968754995E-4	0.0	0.0	2.4856435311663665	8.433199990386152E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5895	0.0	48.76799	1
GTATCAA	10460	0.0	38.59518	1
TCAACGC	11790	0.0	32.87718	4
ATCAACG	11905	0.0	32.5242	3
CAACGCA	12580	0.0	30.774511	5
TATCAAC	13055	0.0	30.272465	2
AACGCAG	13350	0.0	28.970438	6
GTACATG	21760	0.0	27.22352	1
TACATGG	22075	0.0	26.214994	2
CGCAGAG	14660	0.0	25.676455	8
ACGCAGA	14995	0.0	25.290857	7
CATGGGT	10665	0.0	25.009405	4
ACATGGG	22475	0.0	24.61749	3
GCAGAGT	16525	0.0	21.811974	9
ATGGGTG	6125	0.0	19.814613	5
TACCTGG	3550	0.0	19.614567	2
ATGGGTA	4620	0.0	18.734749	5
CAGAGTA	16535	0.0	18.573214	10-11
AGAGTAC	14970	0.0	18.333132	10-11
GTACCTG	4010	0.0	18.304493	1
>>END_MODULE