FastQCFastQC Report
Wed 25 May 2016
SRR2431402_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431402_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4743158
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319000.6725476992332956No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255340.5383333213863No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174100.3670550295815573No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78740.16600754181075142No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52050.1097370148748998No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49990.10539391687985093No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48870.10303262088254281No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118350.064.564341
GTATCAA239150.046.226381
ATCAACG310150.035.4266743
TATCAAC313500.035.1706922
TCAACGC313750.035.019084
CAACGCA324400.033.825935
AACGCAG335000.032.8287476
ATAACGC4450.032.7525563
GTGGTAT39700.032.6908531
TGGTATC38200.032.494972
ACGCAGA376350.029.0969357
CGCAGAG377600.028.9881658
GTACATG206800.027.6269781
GCAGAGT406650.026.6168359
TACATGG211700.026.4969062
ACATGGG213550.025.4508193
CATGGGT102050.025.2002054
TATAACG5050.025.1389982
GAGTACT294600.022.9248912-13
AGAGTAC386900.022.63066710-11
Produced by FastQC (version 0.11.5)