##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431402_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4743158
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.65766668536026	34.0	31.0	34.0	31.0	34.0
2	32.76939709788289	34.0	31.0	34.0	31.0	34.0
3	32.84669391152477	34.0	31.0	34.0	31.0	34.0
4	36.31145325540494	37.0	37.0	37.0	35.0	37.0
5	36.21802668180145	37.0	37.0	37.0	35.0	37.0
6	36.26857718001382	37.0	37.0	37.0	35.0	37.0
7	36.22493747836357	37.0	37.0	37.0	35.0	37.0
8	36.23907763561745	37.0	37.0	37.0	35.0	37.0
9	38.0329832993124	39.0	39.0	39.0	35.0	39.0
10-11	38.01192306897641	39.0	39.0	39.0	35.0	39.0
12-13	37.994660835671084	39.0	39.0	39.0	35.0	39.0
14-15	39.4644748287955	41.0	39.0	41.0	36.0	41.0
16-17	39.50421944620019	41.0	39.0	41.0	36.5	41.0
18-19	39.57367022140102	41.0	40.0	41.0	37.0	41.0
20-21	39.57007419951012	41.0	40.0	41.0	37.0	41.0
22-23	39.506828783692214	41.0	39.5	41.0	36.0	41.0
24-25	39.45991742210569	41.0	39.0	41.0	36.0	41.0
26-27	39.34531571581634	41.0	39.0	41.0	36.0	41.0
28-29	39.26222360714107	41.0	39.0	41.0	36.0	41.0
30-31	39.08419664704401	41.0	39.0	41.0	35.0	41.0
32-33	38.86142903103797	41.0	38.0	41.0	35.0	41.0
34-35	38.72650057198179	41.0	38.0	41.0	35.0	41.0
36-37	38.58022429360354	41.0	38.0	41.0	35.0	41.0
38-39	38.431483623358105	41.0	38.0	41.0	35.0	41.0
40-41	38.29188559605225	41.0	38.0	41.0	34.5	41.0
42-43	38.11895998404438	40.0	38.0	41.0	34.0	41.0
44-45	37.879908069686905	40.0	38.0	41.0	33.5	41.0
46-47	37.53014784664563	40.0	38.0	41.0	33.0	41.0
48-49	37.21100340743446	40.0	38.0	41.0	32.0	41.0
50-51	37.050180386147794	40.0	37.0	41.0	31.5	41.0
52-53	36.997007478983406	40.0	37.0	41.0	32.0	41.0
54-55	36.86748670400607	40.0	37.0	41.0	31.0	41.0
56-57	36.72632547766699	40.0	36.5	41.0	31.0	41.0
58-59	36.560869361720606	40.0	36.0	41.0	31.0	41.0
60-61	36.418032669373446	40.0	36.0	41.0	31.0	41.0
62-63	36.2665716596411	39.5	35.0	41.0	31.0	41.0
64-65	36.043832821930025	39.0	35.0	41.0	30.5	41.0
66-67	35.79269507783633	39.0	35.0	41.0	30.0	41.0
68-69	35.50883577987493	38.5	35.0	41.0	30.0	41.0
70-71	35.21204532929327	37.5	35.0	40.5	29.5	41.0
72-73	34.89945854639461	37.0	35.0	40.0	29.0	41.0
74-75	34.56461791911634	36.5	35.0	39.0	29.0	41.0
76-77	34.22574168939765	36.0	35.0	39.0	29.0	41.0
78-79	33.89945728141462	36.0	35.0	38.5	29.0	40.0
80-81	33.62251088831534	35.0	35.0	37.0	29.0	39.0
82-83	33.407820591260084	35.0	35.0	37.0	29.0	39.0
84-85	33.134026844562214	35.0	34.0	36.5	29.0	39.0
86-87	32.881012080980646	35.0	34.0	36.0	29.0	37.0
88-89	32.63736502136341	35.0	34.0	36.0	29.0	37.0
90-91	32.44884231138832	35.0	34.0	36.0	29.0	37.0
92-93	32.28049961650023	35.0	34.0	35.0	28.5	36.0
94-95	32.13908982159144	35.0	34.0	35.0	27.5	36.0
96-97	31.99544607200519	35.0	34.0	35.0	27.0	36.0
98-99	31.88036683576638	35.0	34.0	35.0	27.0	36.0
100	31.777949627653136	35.0	34.0	35.0	27.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.013192899962518823
1101	2	-0.013295034148761431
1101	3	0.01552087627467813
1101	4	0.05447966098719803
1101	5	0.01644910065886762
1101	6	0.01333312615004445
1101	7	0.01975389798793259
1101	8	0.07604997725844242
1101	9	0.059950388351914796
1101	10-11	0.042245965122873486
1101	12-13	0.04028401737992482
1101	14-15	0.01660995032511181
1101	16-17	0.0020213590562718764
1101	18-19	-0.012471254454027303
1101	20-21	0.028296322009474295
1101	22-23	0.0489699671441457
1101	24-25	0.05573332615776394
1101	26-27	0.07615927332111028
1101	28-29	0.04173221747561229
1101	30-31	0.0103567912170206
1101	32-33	0.042360750402345104
1101	34-35	-0.002676023992307819
1101	36-37	-0.01569329251189089
1101	38-39	-0.030740460035758588
1101	40-41	-0.07998975577904588
1101	42-43	-0.08575607346135428
1101	44-45	-0.12144070562377607
1101	46-47	-0.1356084326431315
1101	48-49	-0.14857860465573935
1101	50-51	-0.1679105372853229
1101	52-53	-0.10898454827722759
1101	54-55	-0.1610358975823587
1101	56-57	-0.10113503614781649
1101	58-59	-0.07802471815097078
1101	60-61	-0.12306981128407557
1101	62-63	-0.10820673979949902
1101	64-65	-0.1301917735978506
1101	66-67	-0.17437345682591143
1101	68-69	-0.1355659457161238
1101	70-71	-0.09790248480664587
1101	72-73	-0.1246087327130283
1101	74-75	-0.1277266202915257
1101	76-77	-0.1624413683525603
1101	78-79	-0.1396532609596548
1101	80-81	-0.07581944960019626
1101	82-83	-0.09991720771306234
1101	84-85	-0.06696661314439467
1101	86-87	-0.08662424136372948
1101	88-89	-0.08238043926716898
1101	90-91	-0.12383009634869069
1101	92-93	-0.17837197473851063
1101	94-95	-0.20337555104534033
1101	96-97	-0.19016155978635751
1101	98-99	-0.15281376303404315
1101	100	-0.09831693071129166
1102	1	-0.08427190861215905
1102	2	-0.04244334234796554
1102	3	-0.053145902700961756
1102	4	-0.0010691756711622702
1102	5	-0.03752433843262537
1102	6	-0.12406207885505438
1102	7	-0.09541638934019403
1102	8	-0.07058882150518997
1102	9	-0.054633896650408076
1102	10-11	-0.039501026218864865
1102	12-13	-0.10982247257584987
1102	14-15	-0.1003052528643309
1102	16-17	-0.09961375798364003
1102	18-19	-0.11375869632291824
1102	20-21	-0.10930091256054908
1102	22-23	-0.09361542679451418
1102	24-25	-0.07001609304748513
1102	26-27	-0.045744144754863214
1102	28-29	-0.12900194075274385
1102	30-31	-0.15154925796231566
1102	32-33	-0.05676222698691191
1102	34-35	-0.0738484470266556
1102	36-37	-0.08264903263651036
1102	38-39	-0.10301399138907641
1102	40-41	-0.18957974113521203
1102	42-43	-0.12218306440691151
1102	44-45	-0.12152288318959137
1102	46-47	-0.13427202844561492
1102	48-49	-0.16779996061644908
1102	50-51	-0.22764656575560593
1102	52-53	-0.24033466672351977
1102	54-55	-0.23444045028834637
1102	56-57	-0.1451152901701036
1102	58-59	-0.15455771449225608
1102	60-61	-0.24305147794770932
1102	62-63	-0.22174875433194075
1102	64-65	-0.21983780447583
1102	66-67	-0.2009523198188461
1102	68-69	-0.1553731152418294
1102	70-71	-0.15547632503036368
1102	72-73	-0.15153449362665583
1102	74-75	-0.07711855293954528
1102	76-77	-0.07909354115138711
1102	78-79	-0.10009137520114564
1102	80-81	-0.13592252898155976
1102	82-83	-0.14075714837733244
1102	84-85	-0.14845617918066267
1102	86-87	-0.14921398879246084
1102	88-89	-0.18152484655806234
1102	90-91	-0.17116425211649045
1102	92-93	-0.20121290770715206
1102	94-95	-0.18886032362345162
1102	96-97	-0.24575642076714388
1102	98-99	-0.17055235856225792
1102	100	-0.10369023981987979
1103	1	-0.09231305048619731
1103	2	-0.10261409301999436
1103	3	-0.06642847737033719
1103	4	-0.017503207490307204
1103	5	-0.044195428597760156
1103	6	-0.02607924510969184
1103	7	-0.03953685093134851
1103	8	0.017297243745481694
1103	9	-0.02440073705052015
1103	10-11	0.0166566865823512
1103	12-13	0.008867069077261647
1103	14-15	-0.012950082101802707
1103	16-17	0.008364905561691671
1103	18-19	-0.0066647617545569915
1103	20-21	-0.0016628182795912494
1103	22-23	-0.023232517744844472
1103	24-25	-0.0288251552514609
1103	26-27	-0.0029012033433204465
1103	28-29	0.006453307255554819
1103	30-31	0.03450045658498624
1103	32-33	0.029773947939055745
1103	34-35	0.009789627751793262
1103	36-37	0.02830503832470299
1103	38-39	-0.004645521755854531
1103	40-41	-0.008200922957776413
1103	42-43	0.07902910916862993
1103	44-45	8.462208903310398E-4
1103	46-47	0.0039942611841397024
1103	48-49	0.04376714819801464
1103	50-51	0.03369225820933508
1103	52-53	0.0936088376518569
1103	54-55	0.16760744121301485
1103	56-57	0.15477359380899713
1103	58-59	0.09241708957554806
1103	60-61	0.10432897404856334
1103	62-63	0.05023938743588019
1103	64-65	0.06438686994856369
1103	66-67	0.08405952708078956
1103	68-69	0.095605766081583
1103	70-71	0.060185806891503546
1103	72-73	0.02602244647084717
1103	74-75	0.08560288077293876
1103	76-77	0.04839556621057994
1103	78-79	0.0691198060301943
1103	80-81	0.09212247034945875
1103	82-83	0.061967853768379655
1103	84-85	0.05409108213467562
1103	86-87	0.07124903430651841
1103	88-89	0.08188697620061447
1103	90-91	0.06167511559087302
1103	92-93	0.04868597789978679
1103	94-95	0.028880692986454903
1103	96-97	0.03589227444868115
1103	98-99	0.007874991097725825
1103	100	0.03207551107484008
1104	1	-0.08748414408915295
1104	2	-0.08886195746874392
1104	3	-0.09848550368584341
1104	4	-0.07503265541444648
1104	5	-0.06374476190003264
1104	6	-0.11954892076551005
1104	7	-0.09832963142390838
1104	8	-0.12814924243163262
1104	9	-0.08246587529094285
1104	10-11	-0.07749801593276828
1104	12-13	-0.07674581527307822
1104	14-15	-0.14474399758647394
1104	16-17	-0.08123164765891744
1104	18-19	-0.08553753712037349
1104	20-21	-0.08357749027150874
1104	22-23	-0.11248660051653303
1104	24-25	-0.0870138630878472
1104	26-27	-0.08197395729614243
1104	28-29	-0.1347639181656035
1104	30-31	-0.07969695502094964
1104	32-33	-0.09600139754971337
1104	34-35	-0.0771764081454549
1104	36-37	-0.11164995053662352
1104	38-39	-0.08725316129676486
1104	40-41	-0.06249997152346509
1104	42-43	-0.11849973745511022
1104	44-45	-0.043117445178459946
1104	46-47	-0.04055247070950685
1104	48-49	-0.055731004511741844
1104	50-51	-0.1280948134538562
1104	52-53	-0.0418918541014861
1104	54-55	0.03273644937552689
1104	56-57	-0.03340377442165021
1104	58-59	0.010603357825758053
1104	60-61	-0.01632735586738221
1104	62-63	-0.025777146562887765
1104	64-65	-0.016366838826307628
1104	66-67	-0.02233004827004237
1104	68-69	-0.05767476385018
1104	70-71	-0.032406041099754646
1104	72-73	0.04681968225981592
1104	74-75	-0.004177103453990583
1104	76-77	0.0017643764107262427
1104	78-79	0.07584638683024991
1104	80-81	0.030153102371201612
1104	82-83	0.0350449507519528
1104	84-85	0.018482934260681816
1104	86-87	0.025873655447504973
1104	88-89	0.06623633886731994
1104	90-91	0.018068135847130407
1104	92-93	0.009580262455678223
1104	94-95	0.021528205319881977
1104	96-97	-0.06372103437932708
1104	98-99	-0.11072525893836982
1104	100	-0.04030016834840566
1105	1	-0.09449417147385475
1105	2	-0.12943527248387454
1105	3	-0.09203181325046472
1105	4	-0.042119195726336045
1105	5	-0.09226416605922338
1105	6	0.015565289835826945
1105	7	0.012432923991489986
1105	8	-0.014047312216845853
1105	9	0.0162931935150894
1105	10-11	-0.07123662475719073
1105	12-13	-0.03307832260721
1105	14-15	-0.06278372952483835
1105	16-17	-0.07483366703053917
1105	18-19	-0.1454956344657674
1105	20-21	-0.03757051341630557
1105	22-23	-0.07122419641615352
1105	24-25	-0.09485329004514398
1105	26-27	-0.06836077439803034
1105	28-29	-0.029249089145238827
1105	30-31	-0.007019325622714234
1105	32-33	-0.05574952917809384
1105	34-35	0.01143926848006771
1105	36-37	0.038902663263925774
1105	38-39	0.03741031235578873
1105	40-41	-0.015645856754836984
1105	42-43	-0.0860540815790074
1105	44-45	-0.04455074117318958
1105	46-47	-0.06435590756695575
1105	48-49	-0.04261718022144123
1105	50-51	-0.04548547857059759
1105	52-53	-0.022884809633389125
1105	54-55	0.052144667880760665
1105	56-57	0.08360437891473538
1105	58-59	0.105892065073661
1105	60-61	0.06517981281138674
1105	62-63	0.06889065532940464
1105	64-65	0.06130562404231199
1105	66-67	0.03190469623886827
1105	68-69	0.13960116824043212
1105	70-71	0.09845215166902932
1105	72-73	0.16984685211239992
1105	74-75	0.10963733455752589
1105	76-77	0.07592445751575383
1105	78-79	0.12160103103020958
1105	80-81	0.13662921626817592
1105	82-83	0.07957385234441006
1105	84-85	0.016214836850593883
1105	86-87	0.06777688089522371
1105	88-89	0.1147767905510193
1105	90-91	0.11679434960949209
1105	92-93	0.0748703591717117
1105	94-95	0.04390996384444179
1105	96-97	0.08336963247530704
1105	98-99	0.10259153510114416
1105	100	0.09594938063472114
1106	1	-0.11220488552367414
1106	2	-0.0821177222675189
1106	3	-0.08331551803170356
1106	4	-0.04278868453326368
1106	5	-0.09303244528765475
1106	6	-0.11945223240079628
1106	7	-0.10860840706329355
1106	8	-0.07708833775613044
1106	9	-0.13978718139211566
1106	10-11	-0.09463317661224124
1106	12-13	-0.12954590371393948
1106	14-15	-0.16200172577585903
1106	16-17	-0.1835813874007428
1106	18-19	-0.11267733397290414
1106	20-21	-0.09000034547829472
1106	22-23	-0.10592992465220163
1106	24-25	-0.03782839706333618
1106	26-27	-0.10738141922842459
1106	28-29	-0.08576370102420583
1106	30-31	-0.14026725422843356
1106	32-33	-0.09834569328482701
1106	34-35	-0.10412564413408631
1106	36-37	-0.08928946140861171
1106	38-39	-0.03703606734674736
1106	40-41	-0.1317275616586997
1106	42-43	-0.14045472131638093
1106	44-45	-0.12954467209386422
1106	46-47	-0.18018020627130937
1106	48-49	-0.09049683568943578
1106	50-51	-0.14481806354607585
1106	52-53	-0.12791213171653482
1106	54-55	-0.15900732559155273
1106	56-57	-0.19308556800051946
1106	58-59	-0.13202807079011336
1106	60-61	-0.1407296342553579
1106	62-63	-0.17437204203609724
1106	64-65	-0.18097906856149137
1106	66-67	-0.18346997541565457
1106	68-69	-0.16937559078220943
1106	70-71	-0.17702630626310878
1106	72-73	-0.1896365099068973
1106	74-75	-0.20312101566202756
1106	76-77	-0.177894363790422
1106	78-79	-0.17649922916416472
1106	80-81	-0.17661369688663342
1106	82-83	-0.17077891518759714
1106	84-85	-0.10109782936326894
1106	86-87	-0.12439055319340042
1106	88-89	-0.22426291429912482
1106	90-91	-0.19309434297765904
1106	92-93	-0.1890627234625626
1106	94-95	-0.19414570268024534
1106	96-97	-0.18954545895359587
1106	98-99	-0.20086012466366654
1106	100	-0.17363041144981395
1107	1	-0.2343726329085669
1107	2	-0.20728856315931665
1107	3	-0.19933323345372145
1107	4	-0.20795922764920505
1107	5	-0.21268462708013658
1107	6	-0.1656741692278061
1107	7	-0.12889630828623666
1107	8	-0.11402703227156508
1107	9	-0.11394035729167484
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2216	24-25	-0.06999317475329292
2216	26-27	-0.005926094034279572
2216	28-29	-6.902254405218855E-4
2216	30-31	-0.062063618436383194
2216	32-33	-0.011846155210442078
2216	34-35	0.02695312641715475
2216	36-37	0.02791359753250333
2216	38-39	0.052825839385306494
2216	40-41	0.09005491265116916
2216	42-43	0.12866521770646955
2216	44-45	0.12763880258524551
2216	46-47	0.1477486220885993
2216	48-49	0.1637914602672197
2216	50-51	0.15546517174237096
2216	52-53	0.1485510158134602
2216	54-55	0.18493413318386587
2216	56-57	0.20801326628608052
2216	58-59	0.17996934104240125
2216	60-61	0.12983604049463082
2216	62-63	0.0768269626365381
2216	64-65	0.10724350889940126
2216	66-67	0.13561417611176552
2216	68-69	0.157616089613704
2216	70-71	0.13717646054527677
2216	72-73	0.1093261964581842
2216	74-75	0.11981357873564491
2216	76-77	0.10860183774261145
2216	78-79	0.1493364261547967
2216	80-81	0.16852792817213214
2216	82-83	0.1794567434926222
2216	84-85	0.2036829400975435
2216	86-87	0.21098570733956734
2216	88-89	0.22050605616284713
2216	90-91	0.19141762976848042
2216	92-93	0.21458584629809963
2216	94-95	0.1779503323717293
2216	96-97	0.19559398128845018
2216	98-99	0.2201296368502419
2216	100	0.23185659259802804
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	50.0
3	288.0
4	89.0
5	158.0
6	404.0
7	888.0
8	1686.0
9	2954.0
10	4275.0
11	6318.0
12	9773.0
13	14421.0
14	16071.0
15	20856.0
16	26627.0
17	39428.0
18	44970.0
19	23098.0
20	13851.0
21	12162.0
22	11832.0
23	11992.0
24	13182.0
25	15384.0
26	18970.0
27	24226.0
28	30292.0
29	38460.0
30	48714.0
31	62232.0
32	79097.0
33	103050.0
34	143662.0
35	235479.0
36	372317.0
37	770873.0
38	1486248.0
39	1027845.0
40	10936.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.44939832246307	21.073325088036743	14.853425305076676	22.623851284423516
2	14.136333955804083	22.060786514319112	41.04710023999948	22.755779289877324
3	16.10969830394268	28.546917871324307	32.27151422334422	23.07186960138879
4	10.846697495634764	18.79960566356845	40.44467841889306	29.90901842190372
5	11.038807505148046	39.05322772957186	35.99698720913776	13.910977556142337
6	26.780153644470623	40.62082688369226	18.89700912345741	13.702010348379709
7	23.568706756131675	35.241541605824644	24.187787967425923	17.001963670617762
8	24.2981574723001	35.36416033368486	21.740979322215285	18.59670287179976
9	25.25681213099464	15.964939799576486	22.71150263664109	36.06674543278779
10-11	22.19834338219389	27.627321291004854	30.314159890941855	19.8601754358594
12-13	24.07558677948181	25.894501711149164	30.291783479675722	19.7381280296933
14-15	21.327398608142214	27.08554660956827	29.045591364570058	22.541463417719466
16-17	18.984355992357834	30.37251763487533	29.481191644891442	21.161934727875394
18-19	18.89313125967842	27.954515507083364	33.64877386465338	19.503579368584834
20-21	20.563419983057702	27.083538014124763	33.32138630001362	19.031655702803913
22-23	19.989441630273006	27.204099211147682	33.45391407599559	19.35254508258372
24-25	19.697005633905263	27.30073795778445	33.407334671049824	19.594921737260464
26-27	19.52246332292816	27.21410690017655	33.46370368746197	19.799726089433317
28-29	19.44954126506145	27.277930945934063	33.56134621843318	19.711181570571306
30-31	19.80540378714168	27.638807924261382	33.12643166837263	19.429356620224304
32-33	19.38736742858685	27.433353770020645	33.54603862786901	19.633240173523497
34-35	19.651527394108633	27.241804062825636	33.29913648747583	19.807532055589906
36-37	20.00541838582702	27.57033887048793	32.69907297110967	19.725169772575377
38-39	19.89003869391538	27.60414393638959	32.76297072624935	19.74284664344568
40-41	19.77770696225623	27.467053301142375	32.768810876561346	19.986428860040057
42-43	19.77126978580129	27.558765101406923	32.53515997042045	20.134805142371327
44-45	20.12006146121336	27.735953755665534	32.10633683831476	20.03764794480635
46-47	20.24055090768716	28.08734441259972	31.477145797922645	20.19495888179047
48-49	20.327859969939826	28.036772164001455	31.09678308922356	20.538584776835155
50-51	20.345928666957402	28.15241489007664	30.84423617196647	20.657420270999488
52-53	20.34147282490676	28.27673919964243	30.77108365151282	20.610704323937988
54-55	20.485186198346977	28.558804291050578	30.535474747410856	20.420534763191586
56-57	20.29743013139082	28.26545543824155	30.58569635426555	20.851418076102078
58-59	20.158789341953785	27.94813435278469	30.95929394444334	20.93378236081819
60-61	20.43507557388016	28.29074908071861	30.586375133430472	20.68780021197076
62-63	20.283282009850904	28.149152097839437	30.819347104583944	20.748218787725712
64-65	20.259654585209827	27.96728896595039	30.980660575162833	20.79239587367695
66-67	20.200423959163807	28.082703766941158	30.731800236783798	20.985072037111237
68-69	20.69895134002235	28.170918536495325	30.457669625987887	20.672460497494438
70-71	20.645485085215594	27.99798909837479	30.528946399710232	20.82757941669938
72-73	20.5000239292302	28.15210683695002	30.62427039566297	20.72359883815681
74-75	20.571518566146803	28.206088567362244	30.38945440205113	20.83293846443982
76-77	20.605742953920338	28.24029428643593	30.248913482098544	20.905049277545185
78-79	20.69828217701598	28.28586340378301	30.330158879414004	20.685695539787
80-81	20.546508087904787	28.20173463441456	30.403107060904063	20.848650216776594
82-83	20.397514074384855	28.201277539332114	30.603629164665954	20.797579221617074
84-85	20.422024834130436	28.201520542589204	30.310738134972425	21.065716488307935
86-87	20.533914952897142	28.301730258466158	30.170000384771278	20.994354403865422
88-89	20.31954146531315	28.139496688775235	30.31554830386567	21.225413542045946
90-91	20.22043755239176	28.135704997056788	30.253386384297904	21.39047106625355
92-93	20.27514205595122	28.306543792384474	30.171742674932826	21.246571476731475
94-95	20.108904936668896	28.182533570940272	30.303781629315257	21.404779863075575
96-97	20.078601706796785	28.20695891383666	30.243072436610746	21.47136694275581
98-99	20.051364642791935	28.133300134552048	30.21842464895662	21.596910573699397
100	20.204981834864242	28.239849787651757	30.013375072103955	21.54179330538005
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3227.0
1	1902.5
2	3603.5
3	5913.5
4	3188.5
5	1562.5
6	3625.0
7	9710.5
8	17134.0
9	18317.5
10	11399.5
11	5330.0
12	4481.5
13	4892.5
14	5523.0
15	6221.5
16	6970.5
17	7820.0
18	8914.5
19	10234.5
20	12134.5
21	14939.5
22	19398.5
23	26204.5
24	34105.0
25	42454.5
26	53690.5
27	67725.5
28	84421.0
29	101406.5
30	113526.0
31	125509.5
32	137381.0
33	147728.0
34	157307.5
35	163580.5
36	166661.0
37	168993.5
38	169503.0
39	166835.5
40	164262.5
41	159707.0
42	153156.0
43	148527.0
44	144186.5
45	139103.0
46	135671.0
47	132628.5
48	128286.5
49	123687.5
50	120137.5
51	114591.0
52	106783.5
53	100502.0
54	95531.5
55	91302.5
56	84481.5
57	75769.0
58	69339.0
59	63753.0
60	56253.5
61	47088.0
62	39274.5
63	31677.0
64	24329.5
65	19240.5
66	15135.5
67	11503.0
68	8698.5
69	6531.5
70	4871.0
71	3805.0
72	2942.0
73	2216.0
74	1926.0
75	1501.5
76	938.0
77	693.0
78	552.0
79	342.5
80	170.5
81	85.5
82	42.5
83	26.0
84	17.0
85	10.0
86	7.5
87	3.5
88	1.0
89	1.0
90	1.5
91	1.0
92	0.5
93	0.5
94	0.5
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005312915993943275
2	0.004870172994448003
3	8.433199990386151E-4
4	0.0
5	0.04298823695099341
6	0.0
7	0.0
8	0.0
9	4.216599995193076E-5
10-11	0.0
12-13	1.054149998798269E-5
14-15	2.108299997596538E-5
16-17	0.0
18-19	6.324899992789614E-5
20-21	0.0
22-23	3.162449996394807E-5
24-25	1.054149998798269E-5
26-27	8.433199990386152E-5
28-29	1.0541499987982689E-4
30-31	6.324899992789614E-5
32-33	0.0011384819987021305
34-35	6.43031499266944E-4
36-37	0.0010119839988463383
38-39	7.379049991587883E-4
40-41	9.487349989184421E-5
42-43	3.267864996274634E-4
44-45	8.433199990386152E-5
46-47	8.433199990386152E-5
48-49	1.0541499987982689E-4
50-51	2.6353749969956725E-4
52-53	5.903239993270307E-4
54-55	6.43031499266944E-4
56-57	0.0019923434977287286
58-59	3.9003549955535954E-4
60-61	0.0010752329987742343
62-63	8.433199990386151E-4
64-65	0.0010330669988223035
66-67	2.6353749969956725E-4
68-69	2.0028849977167111E-4
70-71	2.5299599971158456E-4
72-73	1.0541499987982689E-4
74-75	4.7436749945922106E-4
76-77	5.059919994231691E-4
78-79	6.851974992188748E-4
80-81	5.481579993750999E-4
82-83	0.0011701064986660787
84-85	0.001022525498834321
86-87	0.001697181498065213
88-89	0.0010330669988223035
90-91	9.698179988944075E-4
92-93	9.381934989304595E-4
94-95	6.43031499266944E-4
96-97	8.433199990386152E-5
98-99	2.5299599971158456E-4
100	1.2649799985579228E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4743158.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.46831554430159
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.86045166771325	44.66217097687921
2	16.453087252300676	19.897809433000866
3	4.9427539454679135	8.966400156971863
4	1.9275941185179002	4.662334775995211
5	0.9179417374155107	2.7753195314662764
6	0.5072274805488146	1.8402714807940095
7	0.32165882674140794	1.3615117203106504
8	0.20135160577063113	0.9740313946472272
9	0.14716968722891488	0.8009192777322778
>10	0.6561825364508811	6.8571400661675925
>50	0.03986706590982127	1.6521493050568807
>100	0.022897833422420466	2.525274708568547
>500	0.0010478326158094755	0.44650256900938834
>1k	5.937712832968972E-4	0.7212127768016295
>5k	6.985544509363868E-5	0.2762311674997174
>10k+	1.0478316764045802E-4	1.5807206590984673
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31900	0.6725476992332956	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25534	0.5383333213863	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17410	0.3670550295815573	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7874	0.16600754181075142	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5205	0.1097370148748998	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4999	0.10539391687985093	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4887	0.10303262088254281	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.108299997596538E-5	0.0
10-11	0.0	0.0	0.0	3.162449996394807E-5	0.0
12-13	0.0	0.0	0.0	4.216599995193076E-5	0.0
14-15	0.0	0.0	0.0	6.324899992789614E-5	0.0
16-17	0.0	0.0	0.0	8.433199990386152E-5	4.216599995193076E-5
18-19	0.0	0.0	0.0	8.433199990386152E-5	4.216599995193076E-5
20-21	0.0	0.0	0.0	1.2649799985579228E-4	6.324899992789614E-5
22-23	1.054149998798269E-5	0.0	0.0	2.8462049967553264E-4	6.324899992789614E-5
24-25	2.108299997596538E-5	0.0	0.0	6.11406999302996E-4	6.324899992789614E-5
26-27	4.216599995193076E-5	0.0	0.0	0.0010436084988102864	7.379049991587883E-5
28-29	5.270749993991345E-5	0.0	0.0	0.0021715489975244343	8.433199990386152E-5
30-31	6.324899992789614E-5	0.0	0.0	0.004880714494435986	8.433199990386152E-5
32-33	6.324899992789614E-5	0.0	0.0	0.010446626488090846	8.433199990386152E-5
34-35	6.324899992789614E-5	0.0	0.0	0.018795494478573135	1.054149998798269E-4
36-37	6.324899992789614E-5	0.0	0.0	0.03229915596317896	1.054149998798269E-4
38-39	6.324899992789614E-5	0.0	0.0	0.051758764940995	1.054149998798269E-4
40-41	8.433199990386152E-5	0.0	0.0	0.07471815191482131	1.054149998798269E-4
42-43	8.433199990386152E-5	0.0	0.0	0.09691855088951284	1.054149998798269E-4
44-45	8.433199990386152E-5	0.0	0.0	0.12339879885932536	1.2649799985579228E-4
46-47	8.433199990386152E-5	0.0	0.0	0.15782733782007682	1.2649799985579228E-4
48-49	8.433199990386152E-5	0.0	0.0	0.1959243187766463	1.3703949984377497E-4
50-51	8.433199990386152E-5	0.0	0.0	0.23631934673059596	1.4758099983175766E-4
52-53	8.433199990386152E-5	0.0	0.0	0.27584997168553105	1.4758099983175766E-4
54-55	8.433199990386152E-5	0.0	0.0	0.32122058763380856	1.4758099983175766E-4
56-57	1.054149998798269E-4	0.0	0.0	0.3766688775705975	1.4758099983175766E-4
58-59	1.2649799985579228E-4	0.0	0.0	0.4412671894969554	1.5812249981974035E-4
60-61	1.2649799985579228E-4	0.0	0.0	0.5113259984170884	1.6866399980772304E-4
62-63	1.2649799985579228E-4	0.0	0.0	0.583345526334986	1.6866399980772304E-4
64-65	1.2649799985579228E-4	0.0	0.0	0.6593919072482932	1.6866399980772304E-4
66-67	1.2649799985579228E-4	0.0	1.054149998798269E-5	0.7486994951464826	1.6866399980772304E-4
68-69	1.2649799985579228E-4	0.0	2.108299997596538E-5	0.8518586140288812	1.6866399980772304E-4
70-71	1.3703949984377497E-4	0.0	2.108299997596538E-5	0.9704504888936865	1.6866399980772304E-4
72-73	1.4758099983175766E-4	0.0	2.108299997596538E-5	1.1002058122457652	1.6866399980772304E-4
74-75	1.4758099983175766E-4	0.0	2.108299997596538E-5	1.2291072740988178	1.6866399980772304E-4
76-77	1.4758099983175766E-4	0.0	2.108299997596538E-5	1.3706796189374253	1.6866399980772304E-4
78-79	1.4758099983175766E-4	0.0	2.108299997596538E-5	1.5375304807472152	1.6866399980772304E-4
80-81	1.4758099983175766E-4	0.0	2.108299997596538E-5	1.7256857140327182	1.6866399980772304E-4
82-83	1.4758099983175766E-4	0.0	2.108299997596538E-5	1.929358035300532	1.6866399980772304E-4
84-85	1.4758099983175766E-4	0.0	2.108299997596538E-5	2.1344007515667833	1.7920549979570573E-4
86-87	1.4758099983175766E-4	0.0	2.108299997596538E-5	2.353621785316871	1.8974699978368842E-4
88	1.4758099983175766E-4	0.0	2.108299997596538E-5	2.5307189851149805	1.8974699978368842E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11835	0.0	64.56434	1
GTATCAA	23915	0.0	46.22638	1
ATCAACG	31015	0.0	35.426674	3
TATCAAC	31350	0.0	35.170692	2
TCAACGC	31375	0.0	35.01908	4
CAACGCA	32440	0.0	33.82593	5
AACGCAG	33500	0.0	32.828747	6
ATAACGC	445	0.0	32.752556	3
GTGGTAT	3970	0.0	32.690853	1
TGGTATC	3820	0.0	32.49497	2
ACGCAGA	37635	0.0	29.096935	7
CGCAGAG	37760	0.0	28.988165	8
GTACATG	20680	0.0	27.626978	1
GCAGAGT	40665	0.0	26.616835	9
TACATGG	21170	0.0	26.496906	2
ACATGGG	21355	0.0	25.450819	3
CATGGGT	10205	0.0	25.200205	4
TATAACG	505	0.0	25.138998	2
GAGTACT	29460	0.0	22.92489	12-13
AGAGTAC	38690	0.0	22.630667	10-11
>>END_MODULE