FastQCFastQC Report
Wed 25 May 2016
SRR2431401_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431401_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4141346
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA192590.46504204188686477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50600.049.0077971
GTATCAA90300.038.4568441
TCAACGC104000.032.392764
ATCAACG105750.031.9460813
CAACGCA111850.030.2030985
TATCAAC114650.029.835542
AACGCAG120750.028.1776626
GTACATG205150.027.5930141
TACATGG206800.026.3697742
CGCAGAG132450.025.0844888
ACATGGG211150.024.9797483
ACGCAGA136400.024.496477
CATGGGT89700.024.33854
GCAGAGT150150.021.5959369
GTGGTAT28350.020.581341
CATGGGG97150.019.6146474
TGGTATC27950.019.3592972
ATGGGTG51450.019.2042315
GTCGTAC2001.3947789E-518.8219681
ATGGGTA41100.018.5453995
Produced by FastQC (version 0.11.5)