##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431401_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4141346 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.781032060590928 31.0 31.0 34.0 27.0 34.0 2 30.915254122693444 33.0 31.0 34.0 27.0 34.0 3 31.023877502628373 34.0 31.0 34.0 27.0 34.0 4 34.267482118132605 37.0 35.0 37.0 30.0 37.0 5 34.358473549420886 37.0 35.0 37.0 31.0 37.0 6 34.40752885656016 37.0 35.0 37.0 31.0 37.0 7 34.30850235648023 37.0 35.0 37.0 31.0 37.0 8 34.29585960699734 37.0 35.0 37.0 31.0 37.0 9 35.78463209787349 39.0 37.0 39.0 30.0 39.0 10-11 35.789176634842875 39.0 37.0 39.0 30.0 39.0 12-13 35.72076626777864 39.0 37.0 39.0 30.0 39.0 14-15 37.07531874902507 41.0 38.0 41.0 30.5 41.0 16-17 37.102418392474334 40.5 38.0 41.0 30.5 41.0 18-19 37.13441523601264 40.5 38.0 41.0 30.0 41.0 20-21 37.21239543858446 41.0 38.0 41.0 30.0 41.0 22-23 37.18505203380737 40.0 38.0 41.0 30.0 41.0 24-25 37.2275550026489 40.5 38.0 41.0 30.0 41.0 26-27 37.25334347818318 40.0 38.0 41.0 30.0 41.0 28-29 37.20977805283596 40.0 38.0 41.0 30.0 41.0 30-31 37.13958529424974 40.0 38.0 41.0 30.0 41.0 32-33 37.08069646921557 40.0 38.0 41.0 30.0 41.0 34-35 36.998533206353684 40.0 38.0 41.0 30.0 41.0 36-37 36.866943259510315 40.0 38.0 41.0 30.0 41.0 38-39 36.746644327713746 40.0 37.0 41.0 30.0 41.0 40-41 36.67919391424913 40.0 37.0 41.0 30.0 41.0 42-43 36.518583933822484 40.0 37.0 41.0 29.0 41.0 44-45 36.370817000076784 40.0 36.5 41.0 29.0 41.0 46-47 36.24216233079777 40.0 36.0 41.0 28.5 41.0 48-49 36.105086124173155 40.0 36.0 41.0 28.0 41.0 50-51 35.97340949053762 40.0 35.5 41.0 28.0 41.0 52-53 35.8140468099019 40.0 35.0 41.0 28.0 41.0 54-55 35.653585331918656 39.5 35.0 41.0 28.0 41.0 56-57 35.464429076923295 39.0 35.0 41.0 27.5 41.0 58-59 35.25301556547075 39.0 35.0 41.0 27.0 41.0 60-61 35.02342221586895 39.0 34.0 41.0 26.5 41.0 62-63 34.92940531894703 38.5 34.0 41.0 26.5 41.0 64-65 34.68679397954192 38.0 34.0 40.0 26.0 41.0 66-67 34.43157466195773 37.5 34.0 40.0 26.0 41.0 68-69 34.13901869102461 37.0 34.0 40.0 26.0 41.0 70-71 33.806621566997784 36.5 34.0 40.0 25.5 41.0 72-73 33.46125535031364 36.0 33.0 39.0 25.0 41.0 74-75 33.168710849081435 36.0 33.0 39.0 24.5 41.0 76-77 32.89064123113596 35.0 33.0 38.5 24.0 40.0 78-79 32.53356541568853 35.0 33.0 37.5 23.5 39.5 80-81 32.19382973554975 35.0 33.0 37.0 22.5 39.0 82-83 31.856774343413953 35.0 33.0 36.5 21.5 39.0 84-85 31.549193909419785 35.0 32.0 36.0 20.0 37.5 86-87 31.25919085244266 35.0 32.0 36.0 20.0 37.0 88-89 30.987329481767524 35.0 32.0 35.5 18.5 37.0 90-91 30.75467758549998 35.0 32.0 35.0 17.5 36.0 92-93 30.520116285864546 35.0 32.0 35.0 10.0 36.0 94-95 30.30762220785223 35.0 31.5 35.0 4.5 36.0 96-97 30.10635358649096 35.0 31.0 35.0 2.0 36.0 98-99 29.891646097669692 35.0 31.0 35.0 2.0 35.0 100 29.7222224368599 35.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17019693960203952 1101 2 0.16907544586230472 1101 3 0.18974813589883155 1101 4 0.30174555726448204 1101 5 0.24115760745498704 1101 6 0.2678509413602015 1101 7 0.29734026089240473 1101 8 0.2806728386486341 1101 9 0.2820494936817468 1101 10-11 0.25204095309175756 1101 12-13 0.16966071096450008 1101 14-15 0.2790874145420119 1101 16-17 0.239152055150484 1101 18-19 0.1367961586324853 1101 20-21 0.16246968749089774 1101 22-23 0.1982485098160396 1101 24-25 0.2543023509517397 1101 26-27 0.21728177675914395 1101 28-29 0.1975336802282257 1101 30-31 0.24158850767680207 1101 32-33 0.19295735357417243 1101 34-35 0.1496896433084416 1101 36-37 0.22102157445456783 1101 38-39 0.24090994151366374 1101 40-41 0.10798682990959207 1101 42-43 0.1662305643958888 1101 44-45 0.0994088178803878 1101 46-47 0.09290116580329055 1101 48-49 0.1204695042124726 1101 50-51 0.08359470397284952 1101 52-53 0.016065292643212103 1101 54-55 0.028489894771112745 1101 56-57 -0.009692686348294899 1101 58-59 0.027996793086522587 1101 60-61 -0.020578186509084162 1101 62-63 0.024310278709265276 1101 64-65 0.10600541434503441 1101 66-67 0.09075930787027175 1101 68-69 0.11173609591156008 1101 70-71 0.03216332917027387 1101 72-73 0.0370157256737258 1101 74-75 0.09940388814413836 1101 76-77 0.12280271124944875 1101 78-79 0.16266675651392148 1101 80-81 0.1765260483887161 1101 82-83 0.1463095180687155 1101 84-85 0.08167701011451811 1101 86-87 0.12571538377762081 1101 88-89 0.1245826225762201 1101 90-91 0.11234550190469861 1101 92-93 0.10543483948725196 1101 94-95 0.12500690958837524 1101 96-97 0.1014706673464616 1101 98-99 0.13043360620253708 1101 100 0.09169705095624181 1102 1 0.009565549990742994 1102 2 0.08721289138614097 1102 3 0.08719457673778308 1102 4 0.18733781602276167 1102 5 0.21008555359415482 1102 6 0.13027951533601367 1102 7 0.07183186461298874 1102 8 0.08177868861849902 1102 9 0.07539640292893779 1102 10-11 0.1323847161579792 1102 12-13 0.10459004274327555 1102 14-15 0.1338015242679873 1102 16-17 0.08721932200911198 1102 18-19 0.08376702319415585 1102 20-21 0.0934564260892472 1102 22-23 0.12852248002326405 1102 24-25 0.18898670437186382 1102 26-27 0.15789160415350523 1102 28-29 0.11656958484834234 1102 30-31 0.15515921543743616 1102 32-33 0.2158249864678865 1102 34-35 0.21147127063847648 1102 36-37 0.1645303720525888 1102 38-39 0.16239365489616375 1102 40-41 0.15705996339816863 1102 42-43 0.13105023247604208 1102 44-45 0.1045573846117378 1102 46-47 -0.010158706770177162 1102 48-49 -0.020419010929039416 1102 50-51 -0.07978552628075875 1102 52-53 -0.08659439184687301 1102 54-55 -0.09914103957167697 1102 56-57 -0.07947735983017168 1102 58-59 -0.0746629200242026 1102 60-61 -0.04265816511001219 1102 62-63 -0.042633596017516595 1102 64-65 -0.01848208660696571 1102 66-67 -0.020225705475958478 1102 68-69 -0.06936606256829947 1102 70-71 -0.11755951857985991 1102 72-73 -0.06529205375078106 1102 74-75 -0.04721313373470082 1102 76-77 -0.07936307832343203 1102 78-79 -0.03015785755608391 1102 80-81 -0.16507429924900663 1102 82-83 -0.10565002974923132 1102 84-85 -0.1748550997666385 1102 86-87 -0.17018321284083626 1102 88-89 -0.18640835244909582 1102 90-91 -0.13341212518789902 1102 92-93 -0.12447013054679701 1102 94-95 -0.16276746109314644 1102 96-97 -0.1493987060255435 1102 98-99 -0.1987543567237786 1102 100 -0.16272468749513536 1103 1 -0.21337019539348034 1103 2 -0.19258253259674518 1103 3 -0.20098160962538714 1103 4 -0.08348105216141732 1103 5 -0.044947859214644836 1103 6 -0.03776062674769065 1103 7 -0.15488936690612576 1103 8 -0.08702352615831188 1103 9 -0.10204926391924829 1103 10-11 -0.08464976485528553 1103 12-13 -0.08809709231388752 1103 14-15 -0.1392054299645551 1103 16-17 -0.15426957504023164 1103 18-19 -0.11658234405223311 1103 20-21 -0.19231783534814895 1103 22-23 -0.1922737690147045 1103 24-25 -0.08467140753457869 1103 26-27 -0.13300077352994322 1103 28-29 -0.11927037914006888 1103 30-31 -0.06425402043873873 1103 32-33 -0.06371941176268336 1103 34-35 -0.09200365631198082 1103 36-37 -0.18842655550353982 1103 38-39 -0.11571234870097413 1103 40-41 -0.093431198894244 1103 42-43 -0.1554901187551394 1103 44-45 -0.22124417187704637 1103 46-47 -0.3514518533726019 1103 48-49 -0.2222036739026052 1103 50-51 -0.3142933766423823 1103 52-53 -0.4002384800548242 1103 54-55 -0.39747390548666317 1103 56-57 -0.29581292271176807 1103 58-59 -0.32192061754948753 1103 60-61 -0.3148057323853948 1103 62-63 -0.31054683789064796 1103 64-65 -0.30971530093655986 1103 66-67 -0.3760293944713382 1103 68-69 -0.3615939397205423 1103 70-71 -0.37001867284358525 1103 72-73 -0.3881969363243343 1103 74-75 -0.4029381879842191 1103 76-77 -0.37798003933740887 1103 78-79 -0.3583728450947561 1103 80-81 -0.3253917661250014 1103 82-83 -0.3353818917218767 1103 84-85 -0.3421770559821944 1103 86-87 -0.3521113758327452 1103 88-89 -0.42005156492910345 1103 90-91 -0.3359686023237387 1103 92-93 -0.37875213032608457 1103 94-95 -0.4197290678829191 1103 96-97 -0.4227736123338417 1103 98-99 -0.5547572795662319 1103 100 -0.5164709179482827 1104 1 -0.04317925244566467 1104 2 0.04181818515844782 1104 3 0.03752154955084208 1104 4 0.12771809475918872 1104 5 0.20538285807073464 1104 6 0.17369367500002397 1104 7 0.11560967293590352 1104 8 0.06548523277339058 1104 9 0.10949939847451873 1104 10-11 0.0812016974958425 1104 12-13 0.1320126515632225 1104 14-15 0.060236090380549 1104 16-17 0.08592271932950268 1104 18-19 0.13310983206807947 1104 20-21 0.03109364953122906 1104 22-23 0.14306863841147077 1104 24-25 0.08305546554699816 1104 26-27 0.008339757376390367 1104 28-29 0.07891672476846878 1104 30-31 0.004179801010444351 1104 32-33 -0.0016262958353721046 1104 34-35 0.02077315624568854 1104 36-37 -0.11012055718097713 1104 38-39 -0.03246074909179697 1104 40-41 9.942596649281654E-4 1104 42-43 -0.1585735903305121 1104 44-45 -0.07111863069684432 1104 46-47 -0.2251468581509286 1104 48-49 -0.2724260897376283 1104 50-51 -0.32543046394822284 1104 52-53 -0.4001541868194778 1104 54-55 -0.26486716619130135 1104 56-57 -0.2660639516068315 1104 58-59 -0.3729650061852112 1104 60-61 -0.33115062868026257 1104 62-63 -0.3220602586591568 1104 64-65 -0.31468931279744794 1104 66-67 -0.27513360536777753 1104 68-69 -0.3492392076223041 1104 70-71 -0.3615565875654454 1104 72-73 -0.3369322022137453 1104 74-75 -0.3179062492659668 1104 76-77 -0.33709204707373175 1104 78-79 -0.3039189600354604 1104 80-81 -0.24928532640620915 1104 82-83 -0.32193396395333096 1104 84-85 -0.30553125445078777 1104 86-87 -0.2435944430736079 1104 88-89 -0.2967729357441442 1104 90-91 -0.29740555627335397 1104 92-93 -0.32553281127209743 1104 94-95 -0.3452021594004613 1104 96-97 -0.32304407872570096 1104 98-99 -0.3165483219823386 1104 100 -0.27103705748077545 1105 1 -0.23013064154358887 1105 2 -0.18257944416754412 1105 3 -0.13973064389125156 1105 4 -0.0642454296351147 1105 5 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-0.42653985076934475 1105 66-67 -0.457973471144804 1105 68-69 -0.3466774168163269 1105 70-71 -0.3171223768070561 1105 72-73 -0.31657132758218154 1105 74-75 -0.3771778705327691 1105 76-77 -0.3667331226135744 1105 78-79 -0.3271860491150065 1105 80-81 -0.3176023907845149 1105 82-83 -0.35660628702590813 1105 84-85 -0.4131666679836634 1105 86-87 -0.3533423568492111 1105 88-89 -0.33815459964820604 1105 90-91 -0.33160651373241024 1105 92-93 -0.4217465631910393 1105 94-95 -0.44864937810826433 1105 96-97 -0.4618508959685954 1105 98-99 -0.4935662583849414 1105 100 -0.5231110689622476 1106 1 -0.2862569781908846 1106 2 -0.2373652330758702 1106 3 -0.2774780174689617 1106 4 -0.20726414226199097 1106 5 -0.2001091112931519 1106 6 -0.17858230360941008 1106 7 -0.14149539719474546 1106 8 -0.1794584083122004 1106 9 -0.10444733875667822 1106 10-11 -0.12909774135642493 1106 12-13 -0.13643245315729047 1106 14-15 -0.14033464765780224 1106 16-17 -0.10997355911804618 1106 18-19 -0.19920380367876334 1106 20-21 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2214 4 0.14595765955773032 2214 5 0.2136446009652957 2214 6 0.22241186095904197 2214 7 0.19741593402161328 2214 8 0.2557008484358434 2214 9 0.18181085710744327 2214 10-11 0.2610134376165618 2214 12-13 0.28567218278205786 2214 14-15 0.27313180230736833 2214 16-17 0.2882344433886459 2214 18-19 0.28786029554400017 2214 20-21 0.315061992436398 2214 22-23 0.3684712011251037 2214 24-25 0.44452071536911575 2214 26-27 0.40270183241482016 2214 28-29 0.38162914159943284 2214 30-31 0.39628927028790173 2214 32-33 0.3566797019578374 2214 34-35 0.3692551363140453 2214 36-37 0.3196579460430371 2214 38-39 0.3559125530069025 2214 40-41 0.3317491562277226 2214 42-43 0.3340565997076297 2214 44-45 0.4293570848437156 2214 46-47 0.4433965305719241 2214 48-49 0.45322120673919386 2214 50-51 0.46466042014760944 2214 52-53 0.3704719747426495 2214 54-55 0.44875870232339565 2214 56-57 0.5203292075621917 2214 58-59 0.47693905583486185 2214 60-61 0.49895105441743226 2214 62-63 0.403103830786101 2214 64-65 0.4194944849200084 2214 66-67 0.3963684767977682 2214 68-69 0.40184199390311903 2214 70-71 0.3914074870890971 2214 72-73 0.43267842267601964 2214 74-75 0.4420513139581175 2214 76-77 0.5032602935752237 2214 78-79 0.5177052744666426 2214 80-81 0.4849853210222719 2214 82-83 0.48952023738502604 2214 84-85 0.5055938136819194 2214 86-87 0.507888649393248 2214 88-89 0.5227236566943105 2214 90-91 0.5604680218733087 2214 92-93 0.5048888839357843 2214 94-95 0.46641945741758306 2214 96-97 0.5382672848508747 2214 98-99 0.5580148678180912 2214 100 0.5701834051177386 2215 1 0.053163602312412905 2215 2 0.016826390022135485 2215 3 -0.006334201898440028 2215 4 -0.03930593116836434 2215 5 0.04645867540420312 2215 6 -0.006123806365835094 2215 7 -0.04487425752053298 2215 8 -0.09540665284065142 2215 9 -0.15626396135904486 2215 10-11 -0.1445089315595638 2215 12-13 -0.1321678056504041 2215 14-15 -0.09865605991242177 2215 16-17 -0.04999231196246967 2215 18-19 0.012944340928505937 2215 20-21 -0.10101405959944287 2215 22-23 -0.0571141794965655 2215 24-25 -0.059759499172542974 2215 26-27 -0.10477696419987126 2215 28-29 -0.13755105441609317 2215 30-31 -0.13575698630356925 2215 32-33 -0.1473587691391316 2215 34-35 -0.12317287158440848 2215 36-37 -0.1670862667996431 2215 38-39 -0.15350866348225622 2215 40-41 -0.12275563119619193 2215 42-43 -0.07031183989491296 2215 44-45 -0.036896546956171505 2215 46-47 0.010169684849422822 2215 48-49 0.051567297499168774 2215 50-51 0.08622800212574333 2215 52-53 0.1703168748596866 2215 54-55 0.08146003846427163 2215 56-57 0.08324745761076713 2215 58-59 0.060151329172143164 2215 60-61 0.07241451500873097 2215 62-63 0.029195630035118825 2215 64-65 0.021751397201029476 2215 66-67 -0.007441919525923879 2215 68-69 0.02317799921695496 2215 70-71 -0.0035645881541128688 2215 72-73 0.017988441699195334 2215 74-75 0.08497365331168538 2215 76-77 0.0943653774172688 2215 78-79 0.06333943304129974 2215 80-81 0.11852880547161959 2215 82-83 0.17488549591495328 2215 84-85 0.09581608209396109 2215 86-87 0.08332841912696765 2215 88-89 0.13774383210516206 2215 90-91 0.17523685188022142 2215 92-93 0.13703838983025918 2215 94-95 0.10873743815944437 2215 96-97 0.09482395448473113 2215 98-99 0.10118245250737345 2215 100 0.10598063884976128 2216 1 0.27947511302474126 2216 2 0.1946181400613476 2216 3 0.11328967058301842 2216 4 0.18308529280055552 2216 5 0.1758090740651781 2216 6 0.14550157826691645 2216 7 0.120922686008619 2216 8 0.1936971750210219 2216 9 0.19354665660872428 2216 10-11 0.13568087197824497 2216 12-13 0.17025367629630495 2216 14-15 0.156097892966244 2216 16-17 0.1605440229225792 2216 18-19 0.23496494080357877 2216 20-21 0.1915637628272293 2216 22-23 0.17218112973723265 2216 24-25 0.1545045168315724 2216 26-27 0.15121654084050817 2216 28-29 0.16820309846412584 2216 30-31 0.07069608442514408 2216 32-33 0.10910447076781793 2216 34-35 0.14667121264522365 2216 36-37 0.14921352684061873 2216 38-39 0.2031889427860989 2216 40-41 0.2130283560202244 2216 42-43 0.23712989283195185 2216 44-45 0.35238272014530025 2216 46-47 0.3690847522572085 2216 48-49 0.38503392070044384 2216 50-51 0.3594536540727731 2216 52-53 0.3687431834013424 2216 54-55 0.3727826980705231 2216 56-57 0.39004164170233224 2216 58-59 0.43574499700481084 2216 60-61 0.4681650498473431 2216 62-63 0.3391417342804246 2216 64-65 0.34836186181766493 2216 66-67 0.3476160246759932 2216 68-69 0.3750916416937784 2216 70-71 0.36194605375830946 2216 72-73 0.38458679230227233 2216 74-75 0.4140850362337787 2216 76-77 0.3953575228885313 2216 78-79 0.4555860692024467 2216 80-81 0.40643462652783313 2216 82-83 0.37402983245697285 2216 84-85 0.36847519351034563 2216 86-87 0.3323717189290072 2216 88-89 0.4009382207266192 2216 90-91 0.4041949744459288 2216 92-93 0.40775084142564566 2216 94-95 0.32805663757508796 2216 96-97 0.39096890142664975 2216 98-99 0.30509313967468543 2216 100 0.29605621508599 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 93747.0 3 17753.0 4 12594.0 5 10590.0 6 7641.0 7 6921.0 8 7711.0 9 8037.0 10 8191.0 11 9073.0 12 10173.0 13 11305.0 14 12099.0 15 12344.0 16 12620.0 17 12797.0 18 12903.0 19 12287.0 20 11882.0 21 12546.0 22 14581.0 23 20029.0 24 31624.0 25 42068.0 26 48699.0 27 40611.0 28 38028.0 29 46886.0 30 56064.0 31 64211.0 32 81562.0 33 109278.0 34 152241.0 35 237228.0 36 400335.0 37 727841.0 38 1137817.0 39 586929.0 40 2100.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.158341292443346 21.41397339092923 14.165280475166126 22.262404841461304 2 14.79058025408417 22.16234496508925 40.37442786067245 22.672646920154133 3 17.304869993975714 28.489525242725495 30.69663970657615 23.508965056722644 4 11.85926465664559 19.01277987114654 39.45140513749465 29.676550334713212 5 12.170185358894994 40.15935188865933 34.968426976540954 12.702035775904722 6 27.78611377006307 40.173629227361054 18.89377824574258 13.146478756833291 7 24.74349753937436 35.12462143291448 23.292013575732266 16.839867451978893 8 23.831638171254703 37.14821319317115 21.39970964932714 17.620438986247006 9 24.82554443594211 18.33807592806784 21.723339002540147 35.1130406334499 10-11 22.254719393118354 29.053103110626083 29.523447502234095 19.168729994021465 12-13 23.128542772074383 27.18568964754787 28.94717700708452 20.738590573293227 14-15 21.025541145259865 28.81360109813646 26.379536346410294 23.78132141019338 16-17 20.237745441541055 31.769571289803455 27.162374409804613 20.830308858850877 18-19 20.264451949752267 30.700284044742034 28.902377124279273 20.13288688122643 20-21 22.074096932459298 29.646288326739086 28.1018611710741 20.17775356972752 22-23 21.602326475768354 29.764661689473865 28.129174065580198 20.50383776917758 24-25 21.242618334995047 29.805446398926705 28.13959085555545 20.81234441052279 26-27 20.919980512993515 29.795183442230837 28.235365050305184 21.049470994470465 28-29 20.826847040776528 29.808040376838896 28.406107079044247 20.95900550334033 30-31 21.089175403975155 30.14324304338181 28.063459128342615 20.704122424300422 32-33 20.850813073750327 29.95896208040165 28.337466446148063 20.85275839969996 34-35 21.154085251164133 29.648451286507616 28.288450870863997 20.90901259146425 36-37 21.21899264009095 29.806616595213704 27.990118633913102 20.984272130782248 38-39 21.03931377967589 29.808323664610636 28.219237572177086 20.933124983536384 40-41 20.972794695934144 29.57256920643816 28.36184696462164 21.092789133006058 42-43 20.87369947867981 29.602658897291317 28.41753271421929 21.106108909809578 44-45 21.107351195310024 29.66385047603579 28.26758596487157 20.961212363782607 46-47 21.136926419182394 29.80727392110939 28.094287479493012 20.961512180215202 48-49 21.017760952007038 29.60390680765802 28.20443383952028 21.173898400814657 50-51 20.94255388616726 29.608832427909903 28.188007616528708 21.260606069394125 52-53 21.024674223352484 29.677085928764026 28.124068816865446 21.174171031018048 54-55 21.139883250074888 29.846802125322803 28.04094718025936 20.972367444342954 56-57 21.102627209091327 29.61726261470093 27.91092333834006 21.36918683786768 58-59 21.05997854396204 29.417163331974304 28.228501729074377 21.294356394989272 60-61 21.242622318916407 29.671047106595672 27.933874710677692 21.152455863810225 62-63 21.072617736105933 29.65942924307058 28.066875466228673 21.201077554594818 64-65 21.14879443268867 29.509862546247962 28.109178110875728 21.23216491018764 66-67 20.946083999505575 29.57761250458087 27.998264434080937 21.478039061832618 68-69 21.333551946293706 29.737134025170054 27.830585749423392 21.098728279112848 70-71 21.221225575107912 29.50031963025105 27.94209950257051 21.336355292070525 72-73 21.117386201151504 29.57962400605534 28.181967006141722 21.121022786651437 74-75 21.089906957305622 29.67738988944995 28.05820753473409 21.174495618510335 76-77 21.158436282113772 29.563882697717602 27.88565707465405 21.392023945514577 78-79 21.19716223485363 29.505923610006896 28.144215847233546 21.15269830790593 80-81 21.095620302832426 29.55040110047063 28.132049225373656 21.221929371323288 82-83 21.009695213368285 29.492683668363355 28.34070869444327 21.15691242382509 84-85 21.041502827420295 29.464237809755865 28.065088911843166 21.429170450980667 86-87 21.25593526625095 29.48242122008351 27.997201460412324 21.264442053253212 88-89 21.091583891915132 29.35813129994293 28.13778046252035 21.41250434562159 90-91 21.04049587056479 29.382899928320334 28.047763405862074 21.528840795252805 92-93 21.123784208300613 29.468736784872835 27.9966652570531 21.410813749773457 94-95 21.039549559857658 29.355344337063116 28.166947319811804 21.438158783267426 96-97 21.091279073209495 29.454847380488737 28.036106291947515 21.417767254354253 98-99 21.019447080327915 29.45541674153408 28.012983973278043 21.512152204859962 100 21.12194581979666 29.508793419062503 27.8611904795281 21.50807028161273 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 18475.0 1 13370.0 2 7211.0 3 5155.0 4 3517.5 5 2527.5 6 2101.5 7 2027.0 8 2150.5 9 2230.0 10 1844.0 11 1452.0 12 1408.0 13 1407.5 14 1498.0 15 1641.0 16 1839.5 17 2197.5 18 2814.0 19 3681.5 20 4967.0 21 7176.0 22 10861.5 23 16580.0 24 22948.5 25 29719.0 26 39474.0 27 51929.0 28 67929.0 29 83127.0 30 92852.5 31 102956.5 32 112818.5 33 122781.5 34 132816.0 35 139496.5 36 142407.5 37 143567.5 38 144300.0 39 142904.0 40 142000.5 41 139101.5 42 133189.0 43 129312.5 44 126517.0 45 122831.5 46 121164.0 47 120228.0 48 117336.5 49 114726.0 50 112907.0 51 108002.5 52 101669.0 53 96224.5 54 92824.5 55 91014.0 56 84265.5 57 74929.5 58 68067.5 59 62484.5 60 55144.5 61 46915.0 62 40264.0 63 32902.0 64 25712.0 65 21016.0 66 17029.5 67 13263.5 68 10486.0 69 8311.5 70 6441.5 71 5021.0 72 3915.0 73 3155.0 74 2654.5 75 2143.0 76 1662.5 77 1288.5 78 984.0 79 752.0 80 511.0 81 355.5 82 285.5 83 228.5 84 188.0 85 147.5 86 121.0 87 109.5 88 97.5 89 77.0 90 64.0 91 56.5 92 49.0 93 47.5 94 43.0 95 35.5 96 31.0 97 25.0 98 21.0 99 25.0 100 32.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1863645297929707 2 0.1815593287786145 3 0.19500906227105874 4 0.18491572546703414 5 0.1987035133021969 6 0.08048108030577499 7 0.08147109659516495 8 0.07007383589779748 9 0.06309542839453645 10-11 0.07746273796007384 12-13 0.07647272167068388 14-15 0.07394938746967773 16-17 0.06968748807754774 18-19 0.07460134941634919 20-21 0.07594149341784048 22-23 0.074118414641037 24-25 0.07369584671263883 26-27 0.07736615100501142 28-29 0.07562758581388757 30-31 0.08074669443219669 32-33 0.07780079230279238 34-35 0.07691943633784765 36-37 0.08835291714336353 38-39 0.0828957541823359 40-41 0.07817506675365932 42-43 0.0818574444154147 44-45 0.09277177033747 46-47 0.08273880038035943 48-49 0.08802693617002781 50-51 0.08458602589592852 52-53 0.08366844982283538 54-55 0.08517762099568595 56-57 0.07841653414131541 58-59 0.08651776499717724 60-61 0.08314929493937478 62-63 0.07454098256943516 64-65 0.0770401700316757 66-67 0.0776196917620503 68-69 0.07568795266080158 70-71 0.0717278875032417 72-73 0.0687819853738374 74-75 0.07448061572252114 76-77 0.07932203684502574 78-79 0.07630369449932461 80-81 0.08403065090431952 82-83 0.08650569162779444 84-85 0.08818388997200427 86-87 0.08387369710234306 88-89 0.08597446337495104 90-91 0.08432041176950682 92-93 0.07475830321832563 94-95 0.07443232224498991 96-97 0.07399768094720896 98-99 0.08392199057987428 100 0.09224054208462659 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4141346.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.29711849958572 #Duplication Level Percentage of deduplicated Percentage of total 1 75.2794998394662 46.144164222493046 2 15.107133797729622 18.520475411770583 3 4.799718690602539 8.82626776027621 4 1.8585871486166028 4.557041467622361 5 0.936249338152898 2.8694693312958446 6 0.5302919057888598 1.9503219473106532 7 0.3264353690350427 1.4006683248737906 8 0.2164118060809859 1.0612336097644446 9 0.14928287152889527 0.8235548879458612 >10 0.7259617759896763 7.789187596426133 >50 0.04424180401063767 1.8715791814302916 >100 0.024256320927154178 2.7846258656208622 >500 0.001417468587894324 0.5625433382246687 >1k 4.7248936901806213E-4 0.3740472888207859 >5k 0.0 0.0 >10k+ 3.9374114084821254E-5 0.46481976612446646 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 19259 0.46504204188686477 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 2.4146738765609057E-5 0.0 6 0.0 0.0 0.0 2.4146738765609057E-5 0.0 7 0.0 0.0 0.0 2.4146738765609057E-5 0.0 8 0.0 0.0 0.0 2.4146738765609057E-5 0.0 9 2.4146738765609057E-5 0.0 0.0 7.244021629682716E-5 0.0 10-11 2.4146738765609057E-5 0.0 0.0 9.658695506243621E-5 0.0 12-13 2.4146738765609057E-5 0.0 0.0 1.2073369382804528E-4 0.0 14-15 2.4146738765609057E-5 0.0 0.0 1.328070632108498E-4 1.2073369382804528E-5 16-17 2.4146738765609057E-5 0.0 0.0 1.8110054074206794E-4 2.4146738765609057E-5 18-19 4.829347753121811E-5 0.0 0.0 2.2939401827328605E-4 2.4146738765609057E-5 20-21 7.244021629682716E-5 0.0 0.0 2.8976086518730864E-4 2.4146738765609057E-5 22-23 7.244021629682716E-5 0.0 0.0 4.708614059293766E-4 2.4146738765609057E-5 24-25 7.244021629682716E-5 0.0 0.0 7.123287935854672E-4 2.4146738765609057E-5 26-27 7.244021629682716E-5 0.0 0.0 0.0012194103076632575 2.4146738765609057E-5 28-29 8.45135856796317E-5 0.0 0.0 0.0024871140928577327 2.4146738765609057E-5 30-31 9.658695506243623E-5 0.0 0.0 0.005783143934363369 2.4146738765609057E-5 32-33 9.658695506243623E-5 0.0 0.0 0.011554214499343934 2.4146738765609057E-5 34-35 1.4488043259365432E-4 0.0 0.0 0.021273276852501577 2.4146738765609057E-5 36-37 1.4488043259365432E-4 0.0 0.0 0.03768098584373293 2.4146738765609057E-5 38-39 1.4488043259365432E-4 0.0 0.0 0.06014952626513216 2.4146738765609057E-5 40-41 1.4488043259365432E-4 0.0 0.0 0.08383747699419464 2.4146738765609057E-5 42-43 1.4488043259365432E-4 0.0 0.0 0.10728396033560103 2.4146738765609057E-5 44-45 1.4488043259365432E-4 0.0 0.0 0.13816763921681502 2.4146738765609057E-5 46-47 1.4488043259365432E-4 0.0 0.0 0.17700766852129718 2.4146738765609057E-5 48-49 1.4488043259365432E-4 0.0 0.0 0.21899884723469132 6.0366846914022634E-5 50-51 1.4488043259365432E-4 0.0 0.0 0.26198004223747545 7.244021629682716E-5 52-53 1.4488043259365432E-4 0.0 0.0 0.30389878073457277 7.244021629682716E-5 54-55 1.690271713592634E-4 0.0 0.0 0.3531822745551808 7.244021629682716E-5 56-57 1.9317391012487245E-4 0.0 0.0 0.41400790950575006 7.244021629682716E-5 58-59 2.173206488904815E-4 0.0 0.0 0.48243976716748616 7.244021629682716E-5 60-61 2.173206488904815E-4 0.0 0.0 0.5559424399700001 7.244021629682716E-5 62-63 2.4146738765609056E-4 0.0 0.0 0.6325479687038948 7.244021629682716E-5 64-65 2.4146738765609056E-4 0.0 0.0 0.7154799429943791 7.244021629682716E-5 66-67 2.4146738765609056E-4 0.0 0.0 0.8145902322578216 7.244021629682716E-5 68-69 2.4146738765609056E-4 1.2073369382804528E-5 0.0 0.9290940674843396 9.658695506243623E-5 70-71 2.4146738765609056E-4 2.4146738765609057E-5 0.0 1.0561300601302088 1.328070632108498E-4 72-73 2.4146738765609056E-4 2.4146738765609057E-5 0.0 1.1954205226996248 1.690271713592634E-4 74-75 2.8976086518730864E-4 2.4146738765609057E-5 0.0 1.3355681944952198 1.690271713592634E-4 76-77 2.8976086518730864E-4 3.622010814841359E-5 0.0 1.4910852655151248 1.690271713592634E-4 78-79 2.8976086518730864E-4 4.8293477531218114E-5 0.0 1.6700246731376707 1.690271713592634E-4 80-81 2.8976086518730864E-4 4.8293477531218114E-5 0.0 1.8746199906986762 1.690271713592634E-4 82-83 2.8976086518730864E-4 4.8293477531218114E-5 0.0 2.092290767301259 1.690271713592634E-4 84-85 2.8976086518730864E-4 4.8293477531218114E-5 0.0 2.312545244951762 1.690271713592634E-4 86-87 2.8976086518730864E-4 4.8293477531218114E-5 0.0 2.545525053931741 1.690271713592634E-4 88 2.8976086518730864E-4 4.8293477531218114E-5 0.0 2.7396165401297066 1.690271713592634E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5060 0.0 49.007797 1 GTATCAA 9030 0.0 38.456844 1 TCAACGC 10400 0.0 32.39276 4 ATCAACG 10575 0.0 31.946081 3 CAACGCA 11185 0.0 30.203098 5 TATCAAC 11465 0.0 29.83554 2 AACGCAG 12075 0.0 28.177662 6 GTACATG 20515 0.0 27.593014 1 TACATGG 20680 0.0 26.369774 2 CGCAGAG 13245 0.0 25.084488 8 ACATGGG 21115 0.0 24.979748 3 ACGCAGA 13640 0.0 24.49647 7 CATGGGT 8970 0.0 24.3385 4 GCAGAGT 15015 0.0 21.595936 9 GTGGTAT 2835 0.0 20.58134 1 CATGGGG 9715 0.0 19.614647 4 TGGTATC 2795 0.0 19.359297 2 ATGGGTG 5145 0.0 19.204231 5 GTCGTAC 200 1.3947789E-5 18.821968 1 ATGGGTA 4110 0.0 18.545399 5 >>END_MODULE