FastQCFastQC Report
Wed 25 May 2016
SRR2431401_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431401_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4141346
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269120.649837033660071No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225530.544581399380781No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137320.3315830167293436No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48800.1178360851761722No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42370.10230973214988556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108750.065.603271
GTATCAA213600.044.8056031
GTGGTAT33200.038.6717381
TGGTATC36050.035.4823042
ATCAACG269950.035.224173
TCAACGC270150.035.1806834
TATCAAC275500.034.6003342
CAACGCA278650.034.1919065
AACGCAG291000.032.8340956
CGCAGAG326850.029.0458158
ACGCAGA328550.028.9384387
GCAGAGT352450.026.8427459
GTACATG194050.025.3263021
TACATGG194300.024.5905972
GAGTACT247250.024.03605712-13
ACATGGG196800.023.7283273
CAGAGTA347500.023.54640810-11
CATGGGT89750.022.9499934
GTACTTT267450.022.00114-15
AGAGTAC339300.021.5252310-11
Produced by FastQC (version 0.11.5)