##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431401_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4141346
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62863667995864	34.0	31.0	34.0	31.0	34.0
2	32.75571227325609	34.0	31.0	34.0	31.0	34.0
3	32.86328044070696	34.0	31.0	34.0	31.0	34.0
4	36.32991640881974	37.0	37.0	37.0	35.0	37.0
5	36.22516689018498	37.0	37.0	37.0	35.0	37.0
6	36.265134330722425	37.0	37.0	37.0	35.0	37.0
7	36.2269445730929	37.0	37.0	37.0	35.0	37.0
8	36.24056429962626	37.0	37.0	37.0	35.0	37.0
9	38.03774256002759	39.0	39.0	39.0	35.0	39.0
10-11	38.019050931750215	39.0	39.0	39.0	35.0	39.0
12-13	37.99868786621548	39.0	39.0	39.0	35.0	39.0
14-15	39.473249518393295	41.0	39.0	41.0	36.0	41.0
16-17	39.49946152772553	41.0	39.0	41.0	36.0	41.0
18-19	39.5657075018605	41.0	40.0	41.0	36.5	41.0
20-21	39.56379302767748	41.0	40.0	41.0	37.0	41.0
22-23	39.50861603932634	41.0	39.5	41.0	36.0	41.0
24-25	39.459896854790685	41.0	39.0	41.0	36.0	41.0
26-27	39.34722660217234	41.0	39.0	41.0	36.0	41.0
28-29	39.2702757750741	41.0	39.0	41.0	36.0	41.0
30-31	39.10556966261694	41.0	39.0	41.0	35.0	41.0
32-33	38.89969130809162	41.0	38.0	41.0	35.0	41.0
34-35	38.77117089468014	41.0	38.0	41.0	35.0	41.0
36-37	38.63281128889014	41.0	38.0	41.0	35.0	41.0
38-39	38.491744109282344	41.0	38.0	41.0	35.0	41.0
40-41	38.35729796544409	41.0	38.0	41.0	35.0	41.0
42-43	38.19243658945666	40.0	38.0	41.0	34.0	41.0
44-45	37.96472016585912	40.0	38.0	41.0	33.5	41.0
46-47	37.625311915498	40.0	38.0	41.0	33.0	41.0
48-49	37.31100154394248	40.0	38.0	41.0	32.5	41.0
50-51	37.14631559401219	40.0	37.0	41.0	32.0	41.0
52-53	37.084378484676236	40.0	37.0	41.0	32.0	41.0
54-55	36.944595428636006	40.0	37.0	41.0	32.0	41.0
56-57	36.796772232988985	40.0	36.0	41.0	31.0	41.0
58-59	36.620587606058514	40.0	36.0	41.0	31.0	41.0
60-61	36.47098117375366	40.0	35.0	41.0	31.0	41.0
62-63	36.31328051314718	39.0	35.0	41.0	31.0	41.0
64-65	36.081596659636745	39.0	35.0	41.0	31.0	41.0
66-67	35.821046104334194	39.0	35.0	41.0	30.5	41.0
68-69	35.53341582664187	38.0	35.0	41.0	30.0	41.0
70-71	35.23224562738781	37.0	35.0	40.0	30.0	41.0
72-73	34.914273282164785	37.0	35.0	39.5	29.5	41.0
74-75	34.57749038597596	36.0	35.0	39.0	29.0	41.0
76-77	34.242861620352414	36.0	35.0	39.0	29.0	41.0
78-79	33.92153722485395	35.5	35.0	38.0	29.0	39.5
80-81	33.65639118296322	35.0	34.5	37.0	29.0	39.0
82-83	33.45470627182563	35.0	35.0	37.0	29.0	39.0
84-85	33.1905539889688	35.0	34.0	36.5	29.0	38.0
86-87	32.949094690470204	35.0	34.0	36.0	29.0	37.0
88-89	32.71643663678427	35.0	34.0	36.0	29.0	37.0
90-91	32.53609792565026	35.0	34.0	35.5	29.0	36.5
92-93	32.376051530106395	35.0	34.0	35.0	29.0	36.0
94-95	32.24174531661929	35.0	34.0	35.0	29.0	36.0
96-97	32.103506927457886	35.0	34.0	35.0	29.0	36.0
98-99	31.991905530230994	35.0	34.0	35.0	28.0	36.0
100	31.890952361864958	35.0	34.0	35.0	27.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.029930184380162927
1101	2	-0.01414135249459747
1101	3	-0.01650838195930504
1101	4	0.04241090210416587
1101	5	-0.02430519621157856
1101	6	9.122867030910697E-4
1101	7	0.01325241423391077
1101	8	0.04073449830786302
1101	9	0.02095075539537561
1101	10-11	0.053678804135039115
1101	12-13	0.030033362453217194
1101	14-15	0.015465779932206658
1101	16-17	-0.018142339353211412
1101	18-19	-0.0166492496220485
1101	20-21	0.0385504715327869
1101	22-23	0.026761844486792086
1101	24-25	0.04506566006831747
1101	26-27	0.09147440996139267
1101	28-29	0.06178536069207041
1101	30-31	0.012465150657803292
1101	32-33	0.04158498454564352
1101	34-35	0.01581784529135888
1101	36-37	-0.04654314620192679
1101	38-39	-0.10031865583540878
1101	40-41	-0.018908608645844538
1101	42-43	-0.07031266407394554
1101	44-45	-0.02725835398631915
1101	46-47	-0.06067385215110477
1101	48-49	-0.02763435348181531
1101	50-51	-0.07307947284522953
1101	52-53	0.028865922135238975
1101	54-55	-0.012898507386132962
1101	56-57	0.04724360130980898
1101	58-59	0.023883232420402578
1101	60-61	0.04588903418892443
1101	62-63	0.06160743120770462
1101	64-65	0.0624714861164648
1101	66-67	0.04003110575406055
1101	68-69	0.08624985587794498
1101	70-71	0.11631230311789409
1101	72-73	0.08616508002724288
1101	74-75	0.1017669364942293
1101	76-77	0.08719468427861443
1101	78-79	0.04901046645912999
1101	80-81	0.07408787263508287
1101	82-83	0.03807475520299164
1101	84-85	0.0419552840834001
1101	86-87	0.047984762066036524
1101	88-89	0.09913742099579537
1101	90-91	0.039748853341016854
1101	92-93	0.006773968669627095
1101	94-95	0.010959053761311566
1101	96-97	0.033276473795972095
1101	98-99	0.06473182914128017
1101	100	0.06420039629116303
1102	1	-0.005441306775438193
1102	2	-0.00803339332674824
1102	3	0.023018752424128763
1102	4	0.06158389672254572
1102	5	0.05725638362593344
1102	6	0.021118028455461513
1102	7	-0.003450991315880003
1102	8	0.021704435160145863
1102	9	-0.004272842630498985
1102	10-11	0.0031697445723111173
1102	12-13	-0.0057135579445670714
1102	14-15	-0.004776739341146197
1102	16-17	-7.807020397265774E-4
1102	18-19	-0.030594412649641356
1102	20-21	-0.009773874490619505
1102	22-23	0.006513915912684354
1102	24-25	0.020706806026105085
1102	26-27	-0.015595829465425481
1102	28-29	-0.020502424926519325
1102	30-31	-0.036925772094775766
1102	32-33	-0.02073073252685731
1102	34-35	-0.03381715426974097
1102	36-37	-0.08563463153037532
1102	38-39	-0.07346113415548672
1102	40-41	-0.09701019651602394
1102	42-43	-0.04025983409522382
1102	44-45	-0.09326275624127334
1102	46-47	-0.09385705019897017
1102	48-49	-0.09229086674786657
1102	50-51	-0.14469038636422482
1102	52-53	-0.08754271659247337
1102	54-55	-0.10337140935092037
1102	56-57	-0.11994337426170176
1102	58-59	-0.10512575706468397
1102	60-61	-0.11674810410665515
1102	62-63	-0.12910509419418048
1102	64-65	-0.10000985573128673
1102	66-67	-0.12409922851411181
1102	68-69	-0.15079317304884654
1102	70-71	-0.15485704546006218
1102	72-73	-0.12707678231658548
1102	74-75	-0.09812819176072196
1102	76-77	-0.12149353424432263
1102	78-79	-0.08712178504840296
1102	80-81	-0.09735520659534558
1102	82-83	-0.11541484052218465
1102	84-85	-0.08050443029235055
1102	86-87	-0.05655524115837096
1102	88-89	-0.05082389391764508
1102	90-91	-0.03929438216954395
1102	92-93	0.0018491724035030188
1102	94-95	-0.03414713038313266
1102	96-97	-0.03425097575793501
1102	98-99	-0.04514965823096517
1102	100	-0.025837672306842308
1103	1	-0.1033245070480504
1103	2	-0.13950219511686157
1103	3	-0.13327360826124135
1103	4	-0.039714900792802155
1103	5	-0.06727913193422097
1103	6	-0.044785750620533804
1103	7	-0.08160391464899419
1103	8	-0.06370693000963001
1103	9	-0.03426302426255745
1103	10-11	-0.02631217339585845
1103	12-13	-0.037755775032941585
1103	14-15	-0.09965620996532465
1103	16-17	-0.07772842343404562
1103	18-19	-0.0662921339722331
1103	20-21	-0.08927889652642307
1103	22-23	-0.12560546967979747
1103	24-25	-0.08844239615187632
1103	26-27	-0.09758060775273947
1103	28-29	-0.07488733995594998
1103	30-31	-0.04456292609830825
1103	32-33	-0.10304149853459421
1103	34-35	-0.03941208602006441
1103	36-37	-0.04782236579548993
1103	38-39	-0.09123227109196108
1103	40-41	-0.12514295687306998
1103	42-43	-0.1398984786726558
1103	44-45	-0.15100809076619015
1103	46-47	-0.14305436748731637
1103	48-49	-0.17894705968531355
1103	50-51	-0.24617985269411946
1103	52-53	-0.17888269111142563
1103	54-55	-0.11041506542213853
1103	56-57	-0.17341804296997765
1103	58-59	-0.21759711585416142
1103	60-61	-0.23208312485182603
1103	62-63	-0.22062470484760155
1103	64-65	-0.22918855146247807
1103	66-67	-0.1056558154315752
1103	68-69	-0.16563617356366223
1103	70-71	-0.19386094107213836
1103	72-73	-0.2092683552083372
1103	74-75	-0.16442669401916277
1103	76-77	-0.19296464185022444
1103	78-79	-0.12892798140325112
1103	80-81	-0.065572192928542
1103	82-83	-0.1369407184265441
1103	84-85	-0.17271716297501882
1103	86-87	-0.1294725910908241
1103	88-89	-0.1374722104683883
1103	90-91	-0.1427798167076446
1103	92-93	-0.14260680111990354
1103	94-95	-0.12779302112154767
1103	96-97	-0.14101463704220407
1103	98-99	-0.1408693915436885
1103	100	-0.1309513955663668
1104	1	-0.08513690488801018
1104	2	-0.06722922063154613
1104	3	-0.08729904252213316
1104	4	-0.012709611932621101
1104	5	-0.009720275405513235
1104	6	-0.06279219626600963
1104	7	-0.014348549031922175
1104	8	-0.06953015924593586
1104	9	-0.05971941070369979
1104	10-11	-0.05039034837565026
1104	12-13	-0.04402086121850601
1104	14-15	-0.06680309212236324
1104	16-17	-0.059808119559782824
1104	18-19	-0.06157777030254863
1104	20-21	0.0210130003682778
1104	22-23	-0.009853680382398977
1104	24-25	-0.035338596369811626
1104	26-27	0.010337058118651044
1104	28-29	-0.005096095964105984
1104	30-31	0.049974468417119056
1104	32-33	0.11285290103877799
1104	34-35	0.05498854528052277
1104	36-37	0.0654996226466622
1104	38-39	0.0876434061689153
1104	40-41	0.029579978315211974
1104	42-43	0.06848315832471741
1104	44-45	0.11909851913165426
1104	46-47	0.0679010286505104
1104	48-49	0.10280020712644244
1104	50-51	0.04342454470536694
1104	52-53	0.10808708765921438
1104	54-55	0.15261560514363026
1104	56-57	0.09022378199107806
1104	58-59	0.10507721842551376
1104	60-61	0.06945249086020766
1104	62-63	0.09096174161918924
1104	64-65	0.10404498937327133
1104	66-67	0.10410838419619495
1104	68-69	-0.016318101287389197
1104	70-71	-0.005447330660913963
1104	72-73	0.015545144586504023
1104	74-75	0.045679968206471244
1104	76-77	0.011575879819112345
1104	78-79	0.08034331324643063
1104	80-81	0.01787045997182446
1104	82-83	0.030489649502627003
1104	84-85	0.014045761788665345
1104	86-87	-0.02465199064314305
1104	88-89	0.040811935316362735
1104	90-91	0.03723299449904971
1104	92-93	-0.012720168127302145
1104	94-95	-0.03976392323321676
1104	96-97	0.022089730382568007
1104	98-99	0.02279255117536394
1104	100	0.09303012089510432
1105	1	-0.16315402733746254
1105	2	-0.18217221303132902
1105	3	-0.1591653632508283
1105	4	-0.08811342121553878
1105	5	-0.10651037113862571
1105	6	-0.046137481933854474
1105	7	-0.06875273994752717
1105	8	-0.07359600237458608
1105	9	-0.08189514101229634
1105	10-11	-0.11252894419324377
1105	12-13	-0.11743441067571325
1105	14-15	-0.1374616836512459
1105	16-17	-0.14748754854513635
1105	18-19	-0.17598147492719107
1105	20-21	-0.08740017268637246
1105	22-23	-0.13846174093887242
1105	24-25	-0.14254060447328243
1105	26-27	-0.062004856840630396
1105	28-29	-0.12867677594687876
1105	30-31	-0.15193125801505403
1105	32-33	-0.21137062332547885
1105	34-35	-0.20499904484117337
1105	36-37	-0.18180523210061494
1105	38-39	-0.16858595880574967
1105	40-41	-0.22403560055548155
1105	42-43	-0.3204093440534521
1105	44-45	-0.3100587497581486
1105	46-47	-0.2937751901468175
1105	48-49	-0.315538798766859
1105	50-51	-0.3303916998497982
1105	52-53	-0.2614917626677169
1105	54-55	-0.25544500974513795
1105	56-57	-0.2702903321589858
1105	58-59	-0.251935433874948
1105	60-61	-0.2843007740077397
1105	62-63	-0.262010884372593
1105	64-65	-0.28349855634105836
1105	66-67	-0.30622635034875145
1105	68-69	-0.24525825294674775
1105	70-71	-0.2746871210127537
1105	72-73	-0.17843677941089453
1105	74-75	-0.17241596453715147
1105	76-77	-0.19708802518967872
1105	78-79	-0.17740245606393046
1105	80-81	-0.21654931172847824
1105	82-83	-0.18801947755001436
1105	84-85	-0.18167559827480062
1105	86-87	-0.19888109845111757
1105	88-89	-0.21135642718339653
1105	90-91	-0.20410804964331675
1105	92-93	-0.20536270548894464
1105	94-95	-0.16852034704502472
1105	96-97	-0.17802719366128983
1105	98-99	-0.18795689664236193
1105	100	-0.18773068966179451
1106	1	-0.1619882998800577
1106	2	-0.13057786518771763
1106	3	-0.07884351929996569
1106	4	-0.043108166830869266
1106	5	-0.08408328869948889
1106	6	-0.08135997870870426
1106	7	-0.0890027246470737
1106	8	-0.045694262247202744
1106	9	-0.11482743049507604
1106	10-11	-0.11010442767765483
1106	12-13	-0.13942582179572582
1106	14-15	-0.10827483901805834
1106	16-17	-0.16455112760403523
1106	18-19	-0.13884965515841685
1106	20-21	-0.1436981155147592
1106	22-23	-0.13262532285028072
1106	24-25	-0.07628444093342068
1106	26-27	-0.17326948886562832
1106	28-29	-0.1619430640371533
1106	30-31	-0.17128078568290306
1106	32-33	-0.18280900158680424
1106	34-35	-0.25469146212195426
1106	36-37	-0.24191823183799244
1106	38-39	-0.24097047450174358
1106	40-41	-0.2400174607080885
1106	42-43	-0.3306461603155171
1106	44-45	-0.24956994132296018
1106	46-47	-0.28814545211073295
1106	48-49	-0.22773660468795498
1106	50-51	-0.26162498903835285
1106	52-53	-0.2516795022939746
1106	54-55	-0.2312045184270275
1106	56-57	-0.2121591467732955
1106	58-59	-0.21560199666367197
1106	60-61	-0.19032568569697617
1106	62-63	-0.2274618219993556
1106	64-65	-0.15120954228378025
1106	66-67	-0.1418703897927287
1106	68-69	-0.19924296748842352
1106	70-71	-0.1685103296840751
1106	72-73	-0.14028085348680008
1106	74-75	-0.1859690227068853
1106	76-77	-0.20998061249636635
1106	78-79	-0.22939303553543766
1106	80-81	-0.14809423474206085
1106	82-83	-0.1841880689048594
1106	84-85	-0.13462430109733248
1106	86-87	-0.15535394191358165
1106	88-89	-0.15849325960970617
1106	90-91	-0.17097281649982676
1106	92-93	-0.16339971773077622
1106	94-95	-0.18454787420853336
1106	96-97	-0.19533213827363483
1106	98-99	-0.17392183053835808
1106	100	-0.1787575639611383
1107	1	-0.2543921536693432
1107	2	-0.22550847380888683
1107	3	-0.15997682286592863
1107	4	-0.18392807989800275
1107	5	-0.21733712667214888
1107	6	-0.1776786752625057
1107	7	-0.17282472446742503
1107	8	-0.12292183550812297
1107	9	-0.14536458394498908
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2216	32-33	-2.902429700668563E-5
2216	34-35	0.0665383557127015
2216	36-37	0.08969791857442289
2216	38-39	0.0856273011327815
2216	40-41	0.11835904232628991
2216	42-43	0.15200345526802295
2216	44-45	0.10350181721899787
2216	46-47	0.16697702419713067
2216	48-49	0.15541096260184162
2216	50-51	0.12055502848382105
2216	52-53	0.10563133602843777
2216	54-55	0.10436341861655052
2216	56-57	0.04839454261620091
2216	58-59	0.015620182896327606
2216	60-61	-0.01612199340600995
2216	62-63	-0.056222743583056456
2216	64-65	-0.006134456018713763
2216	66-67	-0.010413815204742605
2216	68-69	-0.0036804388344009453
2216	70-71	0.018496040651676537
2216	72-73	0.03162146096602925
2216	74-75	0.05740205357417949
2216	76-77	0.06726272274434564
2216	78-79	0.0742061249364312
2216	80-81	0.09170389402230228
2216	82-83	0.12060144096010106
2216	84-85	0.11846071083229504
2216	86-87	0.03895457281608827
2216	88-89	0.04192657790985521
2216	90-91	0.0438270830556462
2216	92-93	0.04252745327747931
2216	94-95	0.04181549590444433
2216	96-97	0.032678796873021554
2216	98-99	0.006257198278994736
2216	100	0.0773063436013608
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	46.0
3	249.0
4	75.0
5	123.0
6	298.0
7	658.0
8	1271.0
9	2327.0
10	3501.0
11	4754.0
12	7308.0
13	10518.0
14	12723.0
15	16630.0
16	21086.0
17	31572.0
18	37294.0
19	19371.0
20	11600.0
21	10252.0
22	10030.0
23	10273.0
24	11399.0
25	13257.0
26	16737.0
27	21434.0
28	26843.0
29	34307.0
30	43526.0
31	55168.0
32	70164.0
33	92280.0
34	127961.0
35	207238.0
36	343276.0
37	716126.0
38	1303933.0
39	838524.0
40	7214.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.263878578945565	20.98701454253393	14.714324282423922	23.034782596096587
2	14.28666295432516	21.83078967106175	40.51621509235858	23.36633228225451
3	16.467965306604896	27.934635128475136	32.11220515648804	23.485194408431926
4	11.256074715804958	18.54493683937541	39.677124297269536	30.521864147550097
5	11.515759848773094	38.895367166126135	35.69356017369352	13.895312811407242
6	27.58407532237104	39.92153758705503	18.501931497633862	13.992455592940074
7	24.176777308633472	35.071834133153814	23.546764747499967	17.20462381071275
8	24.64242301898948	35.38975009574182	21.203613511162796	18.764213374105907
9	25.61039951996272	15.93781730331571	22.268852269009223	36.182930907712354
10-11	22.777159889562476	27.464138470922254	29.680253714613556	20.07844792490171
12-13	24.372402112743057	25.714260532686716	29.744001104954766	20.169336249615462
14-15	21.677773564171353	26.904565195055568	28.29859281240843	23.11906842836465
16-17	19.495436990775463	30.200024339912673	29.005473099808615	21.29906556950325
18-19	19.38614108830651	27.902075232850216	32.85520335495072	19.856580323892555
20-21	20.993307489883723	26.919605365019006	32.586506898964736	19.500580246132536
22-23	20.48321999853429	27.0443637433118	32.69071981109512	19.781696447058785
24-25	20.265713224292256	27.11370667183085	32.64189926725488	19.97868083662202
26-27	19.98319142267547	27.152227130458805	32.67586466016539	20.188716786700336
28-29	19.863336537609435	27.13596875987754	32.86716790527513	20.1335267972379
30-31	20.207761281102407	27.441145552061112	32.47400991877145	19.877083248065027
32-33	19.94792929356346	27.323175955716234	32.68783027132595	20.04106447939435
34-35	20.23423273103175	27.153664113649107	32.45955958122897	20.15254357409017
36-37	20.348764838484914	27.31730044704556	32.079174162802246	20.25476055166728
38-39	20.253151719346846	27.385989605706985	32.1358864378937	20.22497223705247
40-41	20.23465101827512	27.225960476816507	32.1484475501477	20.390940954760673
42-43	20.223669798673278	27.290714476129395	31.982518651022083	20.50309707417524
44-45	20.559966165536537	27.455488737400064	31.53687351640695	20.44767158065645
46-47	20.660117017637884	27.75470219009951	30.981627571645564	20.603553220617037
48-49	20.72641446333446	27.76594586149752	30.53531452734754	20.972325147820474
50-51	20.692853790763873	27.932740537442573	30.322537258814215	21.051868412979342
52-53	20.749968246854504	28.07393387908499	30.258441628059835	20.917656246000675
54-55	20.834653869748205	28.24575173625637	30.131254377389567	20.788340016605858
56-57	20.74827788293878	27.972947422637844	30.052720554979643	21.226054139443733
58-59	20.689943269377974	27.689307724144392	30.451780420087672	21.168968586389962
60-61	20.821166274881953	27.92153681025667	30.227138546907316	21.030158367954062
62-63	20.671683597702177	27.854398728904624	30.398315991799695	21.075601681593508
64-65	20.676580798175642	27.767697729260465	30.45089288517067	21.104828587393214
66-67	20.63058168440853	27.844342464446854	30.231966262086985	21.293109589057636
68-69	21.0267062078088	27.91867753931366	30.014581027568248	21.040035225309293
70-71	20.928460577605456	27.781123448237395	30.01581131126913	21.274604662888017
72-73	20.829146887445397	27.861458402000704	30.178063095987508	21.131331614566392
74-75	20.8988040181821	27.99569847866341	29.924670253521484	21.180827249632998
76-77	20.901907433928724	27.929758195629756	29.827303979911733	21.341030390529788
78-79	20.923512979083707	27.905434430641268	30.00799021180943	21.163062378465593
80-81	20.835404430491845	27.96314994469763	30.006107950755357	21.195337674055168
82-83	20.7037824409472	27.900965253855436	30.22749652644801	21.16775577874935
84-85	20.682768283649892	27.951145804973194	29.89320388935607	21.47288202202085
86-87	20.811879124855434	27.958724853647354	29.84040085176533	21.38899516973188
88-89	20.670519552866228	27.807800476708945	29.960391769659417	21.56128820076541
90-91	20.566442288897132	27.870867742277827	29.818418326882266	21.744271641942774
92-93	20.590189775607342	27.942847580670087	29.830787924213386	21.636174719509178
94-95	20.492871778458102	27.8157896738492	29.98329880529652	21.708039742396178
96-97	20.515446628935727	27.94155660467909	29.79876926323735	21.744227503147833
98-99	20.441273483990173	27.87582594199161	29.797297101512264	21.885603472505945
100	20.52318319114509	27.922984366658042	29.602681836632144	21.95115060556472
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2290.0
1	1370.5
2	1936.0
3	3333.0
4	2003.5
5	1150.5
6	2978.0
7	7803.5
8	13955.0
9	15593.5
10	10084.0
11	4718.0
12	3792.5
13	4095.5
14	4592.5
15	5150.5
16	5681.0
17	6270.0
18	6998.5
19	7760.5
20	9047.5
21	11023.5
22	14331.0
23	19856.0
24	25763.0
25	32223.0
26	41115.0
27	52551.0
28	68051.0
29	83388.5
30	93072.5
31	101988.5
32	111472.0
33	121063.5
34	131102.5
35	137707.0
36	140107.0
37	141646.5
38	142531.5
39	141159.0
40	140814.0
41	138407.0
42	132778.5
43	129338.0
44	126518.0
45	123299.5
46	121902.5
47	120545.5
48	116655.0
49	113227.5
50	111866.0
51	107662.5
52	100779.5
53	94671.5
54	90593.0
55	87730.0
56	80943.0
57	71880.0
58	65580.0
59	60657.0
60	53334.0
61	44718.0
62	38152.0
63	31151.5
64	23969.5
65	19250.5
66	15435.5
67	11819.0
68	9228.5
69	7028.0
70	5170.0
71	3995.0
72	3093.5
73	2477.0
74	2117.0
75	1645.0
76	1075.0
77	781.5
78	615.0
79	372.5
80	192.0
81	93.5
82	54.5
83	38.5
84	20.5
85	10.5
86	13.5
87	12.5
88	5.0
89	4.5
90	7.0
91	3.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	1.0
98	1.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006084978168933482
2	0.004925934708184247
3	9.417228118587532E-4
4	0.0
5	0.04517854823045454
6	0.0
7	0.0
8	0.0
9	2.4146738765609053E-5
10-11	0.0
12-13	0.0
14-15	1.2073369382804527E-5
16-17	0.0
18-19	8.45135856796317E-5
20-21	0.0
22-23	6.036684691402264E-5
24-25	3.622010814841358E-5
26-27	1.9317391012487243E-4
28-29	1.2073369382804528E-4
30-31	1.5695380197645886E-4
32-33	0.0013039238933428888
34-35	9.055027037103395E-4
36-37	0.001098676613835212
38-39	8.45135856796317E-4
40-41	1.0866032444524075E-4
42-43	4.34641297780963E-4
44-45	1.5695380197645886E-4
46-47	1.0866032444524075E-4
48-49	1.2073369382804528E-4
50-51	2.535407570388951E-4
52-53	5.795217303746173E-4
54-55	8.813559649447306E-4
56-57	0.0018592988849518972
58-59	4.829347753121811E-4
60-61	9.900162893899712E-4
62-63	8.69282595561926E-4
64-65	0.0012556304158116709
66-67	2.656141264216996E-4
68-69	1.328070632108498E-4
70-71	1.690271713592634E-4
72-73	1.2073369382804528E-4
74-75	4.950081446949856E-4
76-77	5.553749916090083E-4
78-79	7.002554242026626E-4
80-81	5.433016222262038E-4
82-83	0.0012556304158116709
84-85	0.001050383136303994
86-87	0.001557464650381784
88-89	8.45135856796317E-4
90-91	0.0010020896587727758
92-93	8.20989118030708E-4
94-95	6.157418385230309E-4
96-97	1.328070632108498E-4
98-99	3.380543427185268E-4
100	1.2073369382804528E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4141346.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.945461970920775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.44300401681608	41.25300330298861
2	16.60287795206307	18.909171100540934
3	5.307469090941167	9.06708837840083
4	2.24906196909021	5.122954913242831
5	1.077914723741472	3.069117595435778
6	0.5961895484895441	2.0370173556583056
7	0.38264155520670834	1.5252790091362314
8	0.2490327156488498	1.1345026430797363
9	0.1878475902431127	0.9627361025866454
>10	0.8154126633325374	8.18763595176243
>50	0.054064845280238395	2.14383139611602
>100	0.03146891523503957	3.3431239675625655
>500	0.0018680876197172448	0.6958402590382089
>1k	0.0010189567040584044	1.0207799359724954
>5k	0.0	0.0
>10k+	1.2736958800728556E-4	1.527918088478465
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26912	0.649837033660071	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22553	0.544581399380781	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13732	0.3315830167293436	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4880	0.1178360851761722	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4237	0.10230973214988556	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.8293477531218114E-5
2	0.0	0.0	0.0	0.0	4.8293477531218114E-5
3	0.0	0.0	0.0	4.8293477531218114E-5	4.8293477531218114E-5
4	0.0	0.0	0.0	4.8293477531218114E-5	4.8293477531218114E-5
5	2.4146738765609057E-5	0.0	0.0	4.8293477531218114E-5	4.8293477531218114E-5
6	2.4146738765609057E-5	0.0	0.0	4.8293477531218114E-5	7.244021629682716E-5
7	2.4146738765609057E-5	0.0	0.0	4.8293477531218114E-5	7.244021629682716E-5
8	2.4146738765609057E-5	0.0	0.0	4.8293477531218114E-5	7.244021629682716E-5
9	9.658695506243623E-5	0.0	0.0	7.244021629682716E-5	7.244021629682716E-5
10-11	9.658695506243623E-5	0.0	0.0	7.244021629682716E-5	7.244021629682716E-5
12-13	9.658695506243623E-5	0.0	0.0	7.244021629682716E-5	7.244021629682716E-5
14-15	9.658695506243623E-5	0.0	0.0	7.244021629682716E-5	7.244021629682716E-5
16-17	9.658695506243623E-5	0.0	0.0	1.2073369382804527E-4	1.2073369382804528E-4
18-19	9.658695506243623E-5	0.0	0.0	1.8110054074206794E-4	1.2073369382804528E-4
20-21	9.658695506243623E-5	0.0	0.0	2.656141264216996E-4	1.2073369382804528E-4
22-23	9.658695506243623E-5	0.0	0.0	4.708614059293766E-4	1.2073369382804528E-4
24-25	9.658695506243623E-5	0.0	0.0	7.123287935854672E-4	1.2073369382804528E-4
26-27	9.658695506243623E-5	0.0	0.0	0.0012556304158116709	1.328070632108498E-4
28-29	9.658695506243623E-5	0.0	0.0	0.0025716276785373643	1.4488043259365432E-4
30-31	9.658695506243623E-5	0.0	0.0	0.0059280243669570225	1.4488043259365432E-4
32-33	9.658695506243623E-5	1.2073369382804528E-5	0.0	0.01178360851761722	1.4488043259365432E-4
34-35	1.0866032444524075E-4	2.4146738765609057E-5	0.0	0.021659624672751322	1.690271713592634E-4
36-37	1.2073369382804528E-4	2.4146738765609057E-5	0.0	0.038381241267935594	1.690271713592634E-4
38-39	1.2073369382804528E-4	2.4146738765609057E-5	0.0	0.061163689293287735	1.690271713592634E-4
40-41	1.2073369382804528E-4	2.4146738765609057E-5	0.0	0.08492408023864706	1.690271713592634E-4
42-43	1.2073369382804528E-4	2.4146738765609057E-5	0.0	0.10857581085956111	1.690271713592634E-4
44-45	1.4488043259365432E-4	2.4146738765609057E-5	0.0	0.1399303511467045	1.9317391012487245E-4
46-47	1.4488043259365432E-4	2.4146738765609057E-5	0.0	0.1791567282714364	1.9317391012487245E-4
48-49	1.690271713592634E-4	2.4146738765609057E-5	0.0	0.22137730100310382	1.9317391012487245E-4
50-51	1.690271713592634E-4	2.4146738765609057E-5	0.0	0.2645758166547785	1.9317391012487245E-4
52-53	1.690271713592634E-4	2.4146738765609057E-5	0.0	0.3069533431884223	1.9317391012487245E-4
54-55	1.690271713592634E-4	2.4146738765609057E-5	0.0	0.35670769841495975	1.9317391012487245E-4
56-57	1.9317391012487245E-4	2.4146738765609057E-5	0.0	0.4185474963936846	1.9317391012487245E-4
58-59	2.173206488904815E-4	2.4146738765609057E-5	0.0	0.4881021774080214	1.9317391012487245E-4
60-61	2.4146738765609056E-4	2.4146738765609057E-5	0.0	0.5631743882303	1.9317391012487245E-4
62-63	2.656141264216996E-4	2.4146738765609057E-5	0.0	0.6412166479207484	2.0524727950767697E-4
64-65	2.656141264216996E-4	2.4146738765609057E-5	0.0	0.7252352254556852	2.173206488904815E-4
66-67	2.656141264216996E-4	2.4146738765609057E-5	0.0	0.8261323733877826	2.173206488904815E-4
68-69	2.656141264216996E-4	2.4146738765609057E-5	0.0	0.942410993913573	2.173206488904815E-4
70-71	2.656141264216996E-4	2.4146738765609057E-5	0.0	1.071004451209824	2.173206488904815E-4
72-73	2.776874958045041E-4	2.4146738765609057E-5	0.0	1.2126492208088868	2.173206488904815E-4
74-75	3.380543427185268E-4	2.4146738765609057E-5	0.0	1.354583751273137	2.173206488904815E-4
76-77	3.380543427185268E-4	2.4146738765609057E-5	0.0	1.5128414771429386	2.173206488904815E-4
78-79	3.380543427185268E-4	2.4146738765609057E-5	0.0	1.6944973928766154	2.173206488904815E-4
80-81	3.380543427185268E-4	2.4146738765609057E-5	0.0	1.9026905745136968	2.173206488904815E-4
82-83	3.6220108148413583E-4	2.4146738765609057E-5	0.0	2.1246353238777926	2.173206488904815E-4
84-85	3.6220108148413583E-4	2.4146738765609057E-5	0.0	2.34865669277573	2.173206488904815E-4
86-87	3.6220108148413583E-4	2.4146738765609057E-5	0.0	2.586272675598706	2.173206488904815E-4
88	3.6220108148413583E-4	2.4146738765609057E-5	0.0	2.784191419891021	2.173206488904815E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10875	0.0	65.60327	1
GTATCAA	21360	0.0	44.805603	1
GTGGTAT	3320	0.0	38.671738	1
TGGTATC	3605	0.0	35.482304	2
ATCAACG	26995	0.0	35.22417	3
TCAACGC	27015	0.0	35.180683	4
TATCAAC	27550	0.0	34.600334	2
CAACGCA	27865	0.0	34.191906	5
AACGCAG	29100	0.0	32.834095	6
CGCAGAG	32685	0.0	29.045815	8
ACGCAGA	32855	0.0	28.938438	7
GCAGAGT	35245	0.0	26.842745	9
GTACATG	19405	0.0	25.326302	1
TACATGG	19430	0.0	24.590597	2
GAGTACT	24725	0.0	24.036057	12-13
ACATGGG	19680	0.0	23.728327	3
CAGAGTA	34750	0.0	23.546408	10-11
CATGGGT	8975	0.0	22.949993	4
GTACTTT	26745	0.0	22.001	14-15
AGAGTAC	33930	0.0	21.52523	10-11
>>END_MODULE