FastQCFastQC Report
Wed 25 May 2016
SRR2431400_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431400_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2679199
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA262470.9796584725509379No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29950.11178714235112808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39450.048.1908571
TACCTGG31800.044.980892
GTACCTG35700.040.5987631
GTATCAA71400.039.3465271
TATAACG2950.036.6849062
ACCTGGG39350.035.2743343
TCAACGC85650.031.859694
ATCAACG85800.031.6972893
TATCAAC93850.029.7806192
CAACGCA91950.029.7785875
AACGCAG98250.028.269956
GTACATG161700.027.5885331
TACATGG160500.026.384512
TCTGTCG6650.026.1482188
CATGGGT66000.025.8763854
ATAACGC3900.025.3358963
CGCAGAG110250.024.7662128
ACATGGG164650.024.6621323
ACGCAGA112700.024.5201747
GTACACG3750.023.8425451
Produced by FastQC (version 0.11.5)