##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431400_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2679199
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.1554475796684	31.0	31.0	34.0	26.0	34.0
2	30.29018635793758	33.0	31.0	34.0	26.0	34.0
3	30.42292491151273	33.0	31.0	34.0	26.0	34.0
4	33.54449781445872	37.0	35.0	37.0	26.0	37.0
5	33.71754207134296	37.0	35.0	37.0	28.0	37.0
6	33.70048884013468	37.0	35.0	37.0	28.0	37.0
7	33.60515251013456	37.0	35.0	37.0	28.0	37.0
8	33.540471611104664	37.0	35.0	37.0	27.0	37.0
9	34.92478572886896	39.0	37.0	39.0	25.0	39.0
10-11	34.94047381325538	39.0	37.0	39.0	26.5	39.0
12-13	34.85871374242824	39.0	37.0	39.0	26.0	39.0
14-15	36.1497889854393	40.0	37.5	41.0	26.0	41.0
16-17	36.17811536209143	40.0	37.5	41.0	27.0	41.0
18-19	36.199396536054245	40.0	37.0	41.0	27.0	41.0
20-21	36.27513484440686	40.0	37.0	41.0	27.0	41.0
22-23	36.25719907330512	40.0	37.0	41.0	27.0	41.0
24-25	36.31564004764111	40.0	37.0	41.0	27.0	41.0
26-27	36.35854316905911	40.0	37.0	41.0	28.0	41.0
28-29	36.32331715561256	40.0	37.0	41.0	28.0	41.0
30-31	36.25009340478255	40.0	37.0	41.0	28.0	41.0
32-33	36.186348046561676	40.0	37.0	41.0	28.0	41.0
34-35	36.10516034829813	40.0	36.0	41.0	27.0	41.0
36-37	35.96286446060931	40.0	36.0	41.0	27.0	41.0
38-39	35.827643262034655	40.0	36.0	41.0	27.0	41.0
40-41	35.757176865175	40.0	36.0	41.0	26.5	41.0
42-43	35.58799495670161	40.0	35.0	41.0	26.0	41.0
44-45	35.43417622207234	40.0	35.0	41.0	25.0	41.0
46-47	35.28757177051798	40.0	35.0	41.0	25.0	41.0
48-49	35.13674105581556	40.0	35.0	41.0	25.0	41.0
50-51	34.984477263540334	39.5	35.0	41.0	25.0	41.0
52-53	34.802707824241494	39.0	34.0	41.0	24.0	41.0
54-55	34.62287702406577	39.0	34.0	41.0	24.0	41.0
56-57	34.42154856731433	39.0	34.0	41.0	23.0	41.0
58-59	34.18315660762788	38.5	33.5	41.0	22.5	41.0
60-61	33.92704946515731	38.0	33.0	40.0	22.0	41.0
62-63	33.803696739211986	38.0	33.0	40.0	21.5	41.0
64-65	33.53800072335052	37.0	33.0	40.0	20.0	41.0
66-67	33.270789889067586	37.0	33.0	40.0	19.0	41.0
68-69	32.970816464174554	36.0	33.0	40.0	18.0	41.0
70-71	32.642576755216766	36.0	32.0	39.0	16.0	41.0
72-73	32.30748779765892	35.0	32.0	39.0	12.5	41.0
74-75	32.03867107295875	35.0	32.0	39.0	10.5	40.0
76-77	31.791453900960697	35.0	32.0	37.5	7.5	40.0
78-79	31.460130807752616	35.0	31.5	37.0	6.0	39.0
80-81	31.14946108892994	35.0	31.0	37.0	2.0	39.0
82-83	30.842510392098532	35.0	31.0	36.0	2.0	39.0
84-85	30.556534247735982	35.0	31.0	36.0	2.0	37.0
86-87	30.28555195041503	35.0	31.0	35.5	2.0	37.0
88-89	30.02748433393712	35.0	31.0	35.0	2.0	36.5
90-91	29.80786626898562	35.0	30.5	35.0	2.0	36.0
92-93	29.57136815891615	35.0	30.0	35.0	2.0	36.0
94-95	29.35344351054177	34.5	30.0	35.0	2.0	36.0
96-97	29.149050518457194	34.0	30.0	35.0	2.0	35.5
98-99	28.92584966626219	34.0	30.0	35.0	2.0	35.0
100	28.744597545759014	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.44908714834591734
1101	2	0.3979686628222403
1101	3	0.429852984391605
1101	4	0.612167030006411
1101	5	0.4524184315054143
1101	6	0.48270140912774906
1101	7	0.490872103498198
1101	8	0.40171148089125097
1101	9	0.45929416852995075
1101	10-11	0.5374189672250864
1101	12-13	0.42602577564719013
1101	14-15	0.5445960270832728
1101	16-17	0.4607270681973077
1101	18-19	0.36278555118233413
1101	20-21	0.4809281123231912
1101	22-23	0.4517689196083481
1101	24-25	0.41080654318830767
1101	26-27	0.5046801703370107
1101	28-29	0.5248829814862717
1101	30-31	0.5626616393254125
1101	32-33	0.5542579909263168
1101	34-35	0.5449515275750869
1101	36-37	0.5488971276571917
1101	38-39	0.6720844512342765
1101	40-41	0.502713527362225
1101	42-43	0.4823744517581616
1101	44-45	0.5834383933602965
1101	46-47	0.5761641652248102
1101	48-49	0.5780975936833954
1101	50-51	0.5829225664525453
1101	52-53	0.5336972323117024
1101	54-55	0.4531439003377571
1101	56-57	0.4397387691015666
1101	58-59	0.5276033205492681
1101	60-61	0.5443222600980278
1101	62-63	0.4452785421949983
1101	64-65	0.4687955317788379
1101	66-67	0.5477127748320498
1101	68-69	0.5553357008351512
1101	70-71	0.6246684030056286
1101	72-73	0.5700138229810037
1101	74-75	0.5462313981320079
1101	76-77	0.6819004442263612
1101	78-79	0.524159093554946
1101	80-81	0.4976862631877239
1101	82-83	0.4992718867224326
1101	84-85	0.4985774410950299
1101	86-87	0.44426200470277877
1101	88-89	0.37858674247751267
1101	90-91	0.39367067702516323
1101	92-93	0.3795572433767944
1101	94-95	0.33308717214006833
1101	96-97	0.22900855968870815
1101	98-99	0.21596974693072468
1101	100	0.25078303845808847
1102	1	0.05836843780019052
1102	2	0.14051015730976957
1102	3	0.16801712721303375
1102	4	0.12729025915258774
1102	5	0.24989040993514067
1102	6	0.19614488892177917
1102	7	0.13882907009908507
1102	8	0.18725313865250826
1102	9	0.19486159292655714
1102	10-11	0.1343659327431439
1102	12-13	0.1589216478265456
1102	14-15	0.17147519857114446
1102	16-17	0.15434799053513615
1102	18-19	0.16760839068255962
1102	20-21	0.1735925298186487
1102	22-23	0.14547810471936629
1102	24-25	0.09585017355700387
1102	26-27	0.12411249365981547
1102	28-29	0.08598792652594511
1102	30-31	0.14500120446171394
1102	32-33	0.12545777362485921
1102	34-35	0.19414544857667693
1102	36-37	0.17808778460150876
1102	38-39	0.19176038831493258
1102	40-41	0.15549589170188227
1102	42-43	0.16137961160483627
1102	44-45	0.08936440059763129
1102	46-47	0.049370903781053244
1102	48-49	0.010706964404903374
1102	50-51	0.008981145721655537
1102	52-53	-0.037037461201393285
1102	54-55	-0.054740293234580406
1102	56-57	-0.07174304486956373
1102	58-59	-0.07525129808468733
1102	60-61	0.018096736275651892
1102	62-63	0.07021486969262014
1102	64-65	0.0958970432513695
1102	66-67	0.07514991869831533
1102	68-69	0.02870365802569097
1102	70-71	-0.058744190841338195
1102	72-73	-0.07963027005327916
1102	74-75	-0.0894091380294384
1102	76-77	0.0160831103956518
1102	78-79	-0.04223286600759124
1102	80-81	-0.11388167342352062
1102	82-83	-0.05162458809571646
1102	84-85	-0.041939388287893564
1102	86-87	-0.12018665024483965
1102	88-89	-0.0989164195336798
1102	90-91	-0.12547359288898718
1102	92-93	-0.042475086522312466
1102	94-95	-0.04218208069553597
1102	96-97	0.030353009714190193
1102	98-99	-0.031153545710790098
1102	100	-0.030409046847896803
1103	1	-0.11658046352111029
1103	2	-0.06983597829752597
1103	3	-0.07338384776121387
1103	4	0.02015400123388389
1103	5	0.023424847257174974
1103	6	0.04971432517513108
1103	7	0.05641240640193956
1103	8	0.07510671160299154
1103	9	0.09102505753119061
1103	10-11	0.05206471154077974
1103	12-13	0.0915341209716658
1103	14-15	0.03938174925974636
1103	16-17	-0.024192103644857355
1103	18-19	0.004357486453301362
1103	20-21	-0.0988311293071007
1103	22-23	-0.069047962898793
1103	24-25	-0.025504382640228584
1103	26-27	-0.09601199024898222
1103	28-29	-0.13635628637517527
1103	30-31	-0.09692504264932467
1103	32-33	-0.1497172825308013
1103	34-35	-0.24020815041426857
1103	36-37	-0.24977917616414658
1103	38-39	-0.18569928359784882
1103	40-41	-0.16348177205549064
1103	42-43	-0.2882106044381487
1103	44-45	-0.3098291747130091
1103	46-47	-0.43712197290746246
1103	48-49	-0.3642068930029225
1103	50-51	-0.48555348678077337
1103	52-53	-0.4826780328037472
1103	54-55	-0.4891630874377668
1103	56-57	-0.4336326343321488
1103	58-59	-0.42675162529475585
1103	60-61	-0.3850301071582578
1103	62-63	-0.2822612892358194
1103	64-65	-0.3298829913188257
1103	66-67	-0.3855875441237657
1103	68-69	-0.3678897376233934
1103	70-71	-0.40136201756625667
1103	72-73	-0.4690837318978325
1103	74-75	-0.3889307527652548
1103	76-77	-0.42747215155760543
1103	78-79	-0.43472729753255024
1103	80-81	-0.44291987824739465
1103	82-83	-0.3821881801123119
1103	84-85	-0.4056842458255261
1103	86-87	-0.37334698078780093
1103	88-89	-0.406682772077275
1103	90-91	-0.4860999216637296
1103	92-93	-0.499761679737329
1103	94-95	-0.5344200225424593
1103	96-97	-0.5223336797767075
1103	98-99	-0.5637568607694199
1103	100	-0.5643098160639077
1104	1	-0.29900646290536415
1104	2	-0.19991263977183849
1104	3	-0.23998165722607823
1104	4	-0.234756523418298
1104	5	-0.16458679905554874
1104	6	-0.2700835029619455
1104	7	-0.32752145618728434
1104	8	-0.2867504780129053
1104	9	-0.30822412518310927
1104	10-11	-0.31578233594317595
1104	12-13	-0.27037933168585937
1104	14-15	-0.33334050447069075
1104	16-17	-0.36819255374918924
1104	18-19	-0.3515749721608543
1104	20-21	-0.3887509209339868
1104	22-23	-0.32268798319729797
1104	24-25	-0.23822676368120455
1104	26-27	-0.2555521076457197
1104	28-29	-0.2794720788633853
1104	30-31	-0.3411388816327161
1104	32-33	-0.3309321206965308
1104	34-35	-0.3483387348804925
1104	36-37	-0.43126667105289584
1104	38-39	-0.44119502601880356
1104	40-41	-0.3941421044071589
1104	42-43	-0.4909058094717551
1104	44-45	-0.4763996740153402
1104	46-47	-0.5261040950864242
1104	48-49	-0.5925157108452268
1104	50-51	-0.5928299270906905
1104	52-53	-0.5968238003514159
1104	54-55	-0.5091746945990749
1104	56-57	-0.4803556338559005
1104	58-59	-0.5472135352840155
1104	60-61	-0.5889063911636825
1104	62-63	-0.6384458363929397
1104	64-65	-0.5482029632064709
1104	66-67	-0.5375619593611631
1104	68-69	-0.5518609857032999
1104	70-71	-0.6858679972598232
1104	72-73	-0.7153235879520565
1104	74-75	-0.616604386877249
1104	76-77	-0.5862475844031465
1104	78-79	-0.5468754190930305
1104	80-81	-0.4654235548523573
1104	82-83	-0.5642208515540794
1104	84-85	-0.4346672071588529
1104	86-87	-0.38305985036308954
1104	88-89	-0.49684720526546045
1104	90-91	-0.45740464106515333
1104	92-93	-0.5162220075167738
1104	94-95	-0.5090827625690153
1104	96-97	-0.5260201410408349
1104	98-99	-0.5975660548622628
1104	100	-0.5355101202950117
1105	1	-0.31698747411439143
1105	2	-0.2657759626337146
1105	3	-0.18004597858571358
1105	4	-0.178024206843574
1105	5	-0.1918924814622116
1105	6	-0.22550820690072726
1105	7	-0.281024758120509
1105	8	-0.2499855754445406
1105	9	-0.11613942941891509
1105	10-11	-0.28423207508402726
1105	12-13	-0.1904209348675252
1105	14-15	-0.24959280223530556
1105	16-17	-0.332977171084444
1105	18-19	-0.2646638311856364
1105	20-21	-0.26180303712437336
1105	22-23	-0.3745818200522635
1105	24-25	-0.39532605386185793
1105	26-27	-0.34712779103675473
1105	28-29	-0.3191142695666116
1105	30-31	-0.24741375847894886
1105	32-33	-0.1961470272237804
1105	34-35	-0.31504903410882434
1105	36-37	-0.30183096887315486
1105	38-39	-0.26412009642294976
1105	40-41	-0.36009820720578745
1105	42-43	-0.3729082404478703
1105	44-45	-0.4017967539550824
1105	46-47	-0.4588546236525275
1105	48-49	-0.549893648453029
1105	50-51	-0.4359089878479736
1105	52-53	-0.42483645330390374
1105	54-55	-0.49138072784952413
1105	56-57	-0.5544351370465535
1105	58-59	-0.4686474393760136
1105	60-61	-0.6404976265960158
1105	62-63	-0.5778859629064144
1105	64-65	-0.4285743557962789
1105	66-67	-0.5047025210322005
1105	68-69	-0.4271474706744769
1105	70-71	-0.4529048251569847
1105	72-73	-0.49911129376114616
1105	74-75	-0.5032488239864392
1105	76-77	-0.4709111691941352
1105	78-79	-0.5038631513304246
1105	80-81	-0.4243850508442435
1105	82-83	-0.3615120037426003
1105	84-85	-0.3626356970219433
1105	86-87	-0.4003192324115332
1105	88-89	-0.31244938811509826
1105	90-91	-0.3248265652169877
1105	92-93	-0.394895555350935
1105	94-95	-0.39431523803455804
1105	96-97	-0.3205559150156958
1105	98-99	-0.38602131238309667
1105	100	-0.3313913170371592
1106	1	-0.22492910958300527
1106	2	-0.24127372870114172
1106	3	-0.13080616569116188
1106	4	-0.16589775476150947
1106	5	-0.10736457517446496
1106	6	-0.08330399966968116
1106	7	-0.10958532447136093
1106	8	-0.21946744345346758
1106	9	-0.07015702481037067
1106	10-11	-0.11698184770253306
1106	12-13	-0.07369941478854969
1106	14-15	-0.20373583944092388
1106	16-17	-0.1841751776375915
1106	18-19	-0.20747790252644904
1106	20-21	-0.2192257222357057
1106	22-23	-0.07758853246802033
1106	24-25	-0.1202807458971833
1106	26-27	-0.2421977043728134
1106	28-29	-0.23166083356802858
1106	30-31	-0.18534126418161634
1106	32-33	-0.2719885006248077
1106	34-35	-0.23403663870949032
1106	36-37	-0.29517416835354027
1106	38-39	-0.35272588139153527
1106	40-41	-0.30684247579357304
1106	42-43	-0.23160281167577068
1106	44-45	-0.3309737947946587
1106	46-47	-0.39703605595914127
1106	48-49	-0.3980899537885705
1106	50-51	-0.3736163310272502
1106	52-53	-0.3960781000120974
1106	54-55	-0.4243144192094448
1106	56-57	-0.3719487954750065
1106	58-59	-0.4439067850663321
1106	60-61	-0.49174370568955794
1106	62-63	-0.42340561509041663
1106	64-65	-0.415948014038257
1106	66-67	-0.3506245905560377
1106	68-69	-0.3267889692998267
1106	70-71	-0.3937461095050594
1106	72-73	-0.37049785732748575
1106	74-75	-0.2672457025408974
1106	76-77	-0.29541052848590965
1106	78-79	-0.3176820222854708
1106	80-81	-0.34165975151903183
1106	82-83	-0.23272524718686327
1106	84-85	-0.2696869384006071
1106	86-87	-0.3194147655305031
1106	88-89	-0.3496042823075953
1106	90-91	-0.32782396094781063
1106	92-93	-0.30196893923305623
1106	94-95	-0.24639182286805905
1106	96-97	-0.26241271154706425
1106	98-99	-0.4053660587310084
1106	100	-0.4570394061002645
1107	1	-0.2721721171839313
1107	2	-0.45727810052514783
1107	3	-0.3872293194384113
1107	4	-0.2603112379942445
1107	5	-0.2817639003658883
1107	6	-0.20680678369050298
1107	7	-0.2359106347072668
1107	8	-0.30621891322113726
1107	9	-0.2320373694554192
1107	10-11	-0.29976947024019296
1107	12-13	-0.26619527965763723
1107	14-15	-0.34196220885507955
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2216	58-59	0.5503229863399213
2216	60-61	0.6084270617585474
2216	62-63	0.5519324448273153
2216	64-65	0.46781185947764214
2216	66-67	0.37310746165322684
2216	68-69	0.39420917748060447
2216	70-71	0.4551915392043355
2216	72-73	0.4536067105278718
2216	74-75	0.41326605567137875
2216	76-77	0.4797643225093644
2216	78-79	0.5271809009369939
2216	80-81	0.4730124104719309
2216	82-83	0.49910442537555255
2216	84-85	0.4660314370707397
2216	86-87	0.4794154580167316
2216	88-89	0.46944985565789565
2216	90-91	0.4519385264473996
2216	92-93	0.46923420826384543
2216	94-95	0.49163223533606626
2216	96-97	0.509063141213673
2216	98-99	0.4844472579592498
2216	100	0.4806450503082651
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	87376.0
3	17192.0
4	12489.0
5	10767.0
6	6715.0
7	5259.0
8	5767.0
9	6235.0
10	6714.0
11	7263.0
12	8451.0
13	9349.0
14	9653.0
15	9611.0
16	9334.0
17	8939.0
18	8911.0
19	8656.0
20	8601.0
21	8805.0
22	10960.0
23	17513.0
24	27756.0
25	34874.0
26	37930.0
27	29194.0
28	27905.0
29	33023.0
30	39129.0
31	43790.0
32	55098.0
33	73403.0
34	102900.0
35	162244.0
36	264581.0
37	470861.0
38	670965.0
39	319697.0
40	1289.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.49780479151034	21.42480864130372	13.541462203643114	21.535924363542826
2	15.310361535148587	22.910697408718956	39.684112851126955	22.094828205005506
3	16.88680695071213	30.249125888833923	29.75485252750959	23.10921463294435
4	11.574569110554455	19.952368489924105	38.268777806856846	30.2042845926646
5	11.76090949338311	40.811511355206164	33.33344550936894	14.094133642041784
6	26.83587763592201	40.69414994048084	19.118219960564588	13.351752463032568
7	25.051060046913985	35.89080545636551	22.36613079141205	16.69200370530845
8	24.375614600345912	37.23556545846151	20.731765160204112	17.657054780988464
9	25.181022419555237	19.639900855470742	20.802126595628277	34.376950129345744
10-11	22.19671394547844	30.145448223043392	28.326313629045497	19.33152420243267
12-13	23.33359914050287	28.4453511353172	27.742200909508718	20.47884881467121
14-15	21.191101566329746	29.807007633231404	25.53133359788977	23.470557202549077
16-17	20.215473663728275	32.627748648667826	25.95526943918361	21.201508248420286
18-19	20.469068462728764	31.158248652710657	27.94751874290593	20.42516414165465
20-21	22.71537593153014	30.255650808205402	26.91683015034273	20.112143109921725
22-23	21.878068466223482	30.565518441703766	27.092607594501754	20.463805497571
24-25	21.36716654938994	30.44104267229595	27.246148169063744	20.94564260925037
26-27	21.054947304178352	30.337008986881415	27.3131947409133	21.29484896802693
28-29	21.115476839644135	30.33235353437264	27.428550619626357	21.12361900635687
30-31	21.40047903408799	30.911107894724555	26.95032705982323	20.73808601136422
32-33	20.84883759366285	30.543646519858868	27.57065694114958	21.036858945328703
34-35	21.197292123815302	30.027396236985854	27.474205144964753	21.30110649423409
36-37	21.574973978231178	30.39768434739944	26.93804512407243	21.089296550296954
38-39	21.280061556505547	30.413788209015713	27.29263432335468	21.01351591112406
40-41	20.99805440422152	30.162897425008282	27.557950479750527	21.281097691019667
42-43	20.89351123575046	30.25193338426896	27.494219316903575	21.360336063077007
44-45	21.330599136819217	30.317305883783447	27.348751447304302	21.003343532093034
46-47	21.220565071441673	30.618246023205426	27.12127771392628	21.039911191426626
48-49	21.07918349081527	30.182723374483277	27.410654035489046	21.327439099212405
50-51	21.07163728027441	29.99789516895518	27.3932274493127	21.537240101457712
52-53	21.117221757703515	30.095321480044383	27.324911983717335	21.462544778534763
54-55	21.24405758210874	30.544261418201664	27.134293134754074	21.077387864935524
56-57	21.000622452320208	30.23538334229443	27.039997370498448	21.72399683488691
58-59	20.971687548512847	29.813912382159074	27.4957547758305	21.71864529349758
60-61	21.455385067060508	30.25906830583641	26.95630038596165	21.329246241141426
62-63	21.177525595459525	30.151548426325565	27.170235763576088	21.500690214638826
64-65	21.174272849221644	29.89939120783775	27.406310828559803	21.520025114380804
66-67	21.035000128859576	29.9994248007252	27.091101480465824	21.8744735899494
68-69	21.582849355928303	30.25089261686936	26.87473820026701	21.29151982693532
70-71	21.53017677246323	29.833987648467414	27.100598080696354	21.535237498373007
72-73	21.19023445443635	29.99594966530118	27.48358438001215	21.33023150025032
74-75	21.11555942495366	30.11247025586955	27.296829521266563	21.475140797910225
76-77	21.249000389572604	30.086042132855834	27.032846236851928	21.632111240719638
78-79	21.350430973708313	30.18141887507038	27.220562735597557	21.24758741562374
80-81	21.14769945759949	30.030801147329665	27.298908214000367	21.52259118107048
82-83	20.954589064492858	30.005679565312484	27.53965376876792	21.500077601426746
84-85	21.107724569921825	30.018279104576013	27.195243510656397	21.678752814845765
86-87	21.29851584475378	30.0486201989818	27.167939243521026	21.484924712743393
88-89	21.026677914990984	29.92645695153353	27.360359816886643	21.686505316588843
90-91	21.02285747689777	29.893751868806557	27.194378106934675	21.889012547361
92-93	21.186086966265066	30.12694135300154	27.08705310247655	21.599918578256847
94-95	21.087051032001728	29.949918167428468	27.31598129242427	21.647049508145535
96-97	21.021304504286466	30.156939178995145	27.131734305008315	21.690022011710074
98-99	20.917814587012945	30.04902703256933	27.20827552254756	21.824882857870165
100	21.012775494957044	30.304632050803136	27.043145311916323	21.639447142323494
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	22847.0
1	17290.0
2	10175.0
3	7136.0
4	4698.5
5	3235.5
6	2534.5
7	2460.0
8	2682.5
9	2624.5
10	2136.5
11	1698.5
12	1551.0
13	1506.0
14	1552.0
15	1625.5
16	1726.5
17	2018.0
18	2414.5
19	2922.5
20	3924.0
21	5430.5
22	7766.5
23	11539.0
24	15670.0
25	19447.5
26	24590.0
27	31602.0
28	40785.0
29	49519.0
30	54106.0
31	58753.5
32	64158.0
33	68916.0
34	74235.5
35	78975.5
36	81010.5
37	81771.0
38	82617.0
39	82656.0
40	83360.5
41	83040.5
42	81192.5
43	80547.5
44	80466.5
45	79568.5
46	79550.5
47	79184.0
48	77440.0
49	76639.0
50	77025.5
51	75441.5
52	70977.5
53	66875.0
54	65006.5
55	63538.5
56	59317.5
57	54205.5
58	50425.5
59	47051.0
60	42560.5
61	36405.0
62	30721.5
63	24886.0
64	19272.0
65	15593.0
66	12440.0
67	9575.5
68	7440.5
69	5864.5
70	4611.5
71	3605.0
72	2918.0
73	2398.0
74	1967.0
75	1627.0
76	1261.0
77	987.5
78	761.5
79	572.5
80	424.5
81	289.5
82	214.5
83	162.5
84	132.0
85	117.5
86	97.0
87	80.5
88	81.0
89	67.5
90	48.0
91	42.5
92	36.5
93	30.5
94	30.5
95	29.5
96	26.0
97	19.5
98	16.0
99	15.5
100	20.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16833389382423627
2	0.16359367109348727
3	0.17796363763945866
4	0.16792332335149424
5	0.1803150867106176
6	0.07128996390339053
7	0.07311886873651416
8	0.06143627255758157
9	0.05568828593919302
10-11	0.06921844924546477
12-13	0.06791208865037648
14-15	0.065747262521373
16-17	0.061809518441892516
18-19	0.06666171493793481
20-21	0.0678001148850832
22-23	0.06576592481558854
24-25	0.06610184611146838
26-27	0.06912513777438706
28-29	0.06640044281891715
30-31	0.07227906549681454
32-33	0.06880787877272275
34-35	0.0680800492983164
36-37	0.07888551764911826
38-39	0.07405198344729153
40-41	0.06979698036614675
42-43	0.07328682938445408
44-45	0.08358841579143618
46-47	0.07390268509356715
48-49	0.07910946517970482
50-51	0.07556362927875085
52-53	0.07448121621424911
54-55	0.07644075710688157
56-57	0.07037551148682872
58-59	0.07737387181765894
60-61	0.07427593097787809
62-63	0.06610184611146838
64-65	0.06899450171487821
66-67	0.06964768201242237
68-69	0.06787476406194538
70-71	0.06410498063040483
72-73	0.061305636498072746
74-75	0.06643776740734825
76-77	0.07114066554966615
78-79	0.06835998371154961
80-81	0.0756196161613975
82-83	0.07679534069697697
84-85	0.07832564882265185
86-87	0.07537700633659539
88-89	0.07686998987383917
90-91	0.07548898010188866
92-93	0.06649375428999488
94-95	0.06647509199577933
96-97	0.0657099379329419
98-99	0.07474248833326677
100	0.08207676995997684
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2679199.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.10218694408313
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.79600559299054	49.722002753759085
2	13.294092918587982	16.77772673201501
3	3.793621275453411	7.181573967561366
4	1.4665134598625622	3.701608260010462
5	0.7167134597773174	2.2613093362104446
6	0.44734945857287683	1.6937237498520026
7	0.2871763759480672	1.2685020152699464
8	0.20147782876852877	1.0170953292831737
9	0.14689980213086132	0.834272889849939
>10	0.774117943283048	8.852878558740361
>50	0.05046520696714607	2.1678914284239585
>100	0.023853185422968107	2.5840740475870114
>500	0.0011817187825163547	0.48436319626161867
>1k	4.726875129869466E-4	0.47437094494840276
>5k	0.0	0.0
>10k+	5.9085939123368326E-5	0.9786067902272048
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26247	0.9796584725509379	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2995	0.11178714235112808	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.732458843109452E-5	0.0
4	0.0	0.0	0.0	3.732458843109452E-5	0.0
5	0.0	0.0	0.0	3.732458843109452E-5	0.0
6	0.0	0.0	0.0	3.732458843109452E-5	0.0
7	0.0	0.0	0.0	3.732458843109452E-5	0.0
8	0.0	0.0	0.0	3.732458843109452E-5	0.0
9	3.732458843109452E-5	0.0	0.0	3.732458843109452E-5	0.0
10-11	3.732458843109452E-5	0.0	0.0	3.732458843109452E-5	0.0
12-13	3.732458843109452E-5	0.0	0.0	3.732458843109452E-5	0.0
14-15	3.732458843109452E-5	0.0	0.0	5.598688264664177E-5	0.0
16-17	3.732458843109452E-5	0.0	0.0	7.464917686218903E-5	1.1197376529328356E-4
18-19	3.732458843109452E-5	0.0	0.0	7.464917686218903E-5	1.1197376529328356E-4
20-21	3.732458843109452E-5	0.0	0.0	2.2394753058656712E-4	1.1197376529328356E-4
22-23	3.732458843109452E-5	0.0	0.0	6.531802975441541E-4	1.1197376529328356E-4
24-25	3.732458843109452E-5	0.0	0.0	0.001436996654597139	1.1197376529328356E-4
26-27	3.732458843109452E-5	0.0	0.0	0.002015527775279104	1.1197376529328356E-4
28-29	3.732458843109452E-5	0.0	0.0	0.0032099146050741283	1.1197376529328356E-4
30-31	3.732458843109452E-5	0.0	0.0	0.005598688264664177	1.1197376529328356E-4
32-33	3.732458843109452E-5	1.866229421554726E-5	0.0	0.009629743815222386	1.306360595088308E-4
34-35	1.4929835372437807E-4	3.732458843109452E-5	0.0	0.016030910731155097	1.4929835372437807E-4
36-37	1.4929835372437807E-4	3.732458843109452E-5	0.0	0.025959251253826237	1.4929835372437807E-4
38-39	1.4929835372437807E-4	3.732458843109452E-5	0.0	0.04174755216017922	1.4929835372437807E-4
40-41	1.4929835372437807E-4	3.732458843109452E-5	0.0	0.06050315784680421	1.4929835372437807E-4
42-43	1.4929835372437807E-4	3.732458843109452E-5	0.0	0.08125562901449276	1.4929835372437807E-4
44-45	1.4929835372437807E-4	3.732458843109452E-5	0.0	0.1047701197260823	1.6796064793992533E-4
46-47	1.4929835372437807E-4	3.732458843109452E-5	0.0	0.13267024957832546	1.866229421554726E-4
48-49	1.866229421554726E-4	3.732458843109452E-5	0.0	0.1649933431596533	1.866229421554726E-4
50-51	2.4260982480211438E-4	3.732458843109452E-5	0.0	0.20220595782545456	1.866229421554726E-4
52-53	2.612721190176616E-4	3.732458843109452E-5	0.0	0.24050098555575752	1.866229421554726E-4
54-55	2.612721190176616E-4	3.732458843109452E-5	0.0	0.2834429245457318	1.866229421554726E-4
56-57	2.612721190176616E-4	3.732458843109452E-5	0.0	0.33166629279870585	1.866229421554726E-4
58-59	2.612721190176616E-4	3.732458843109452E-5	0.0	0.3883063557428918	2.2394753058656712E-4
60-61	2.612721190176616E-4	3.732458843109452E-5	0.0	0.44836161852852285	2.2394753058656712E-4
62-63	2.612721190176616E-4	3.732458843109452E-5	0.0	0.5125412483357898	2.2394753058656712E-4
64-65	2.612721190176616E-4	7.464917686218903E-5	0.0	0.5806212976341063	2.2394753058656712E-4
66-67	2.612721190176616E-4	7.464917686218903E-5	0.0	0.6620262250023234	2.2394753058656712E-4
68-69	2.612721190176616E-4	7.464917686218903E-5	0.0	0.7546658534883001	2.2394753058656712E-4
70-71	2.612721190176616E-4	7.464917686218903E-5	0.0	0.8616567862260325	2.2394753058656712E-4
72-73	2.612721190176616E-4	7.464917686218903E-5	0.0	0.9845479936354111	2.4260982480211438E-4
74-75	2.612721190176616E-4	7.464917686218903E-5	0.0	1.1072525781026346	2.612721190176616E-4
76-77	2.612721190176616E-4	7.464917686218903E-5	0.0	1.2369181983122566	2.612721190176616E-4
78-79	2.7993441323320885E-4	7.464917686218903E-5	0.0	1.3838837652596916	2.612721190176616E-4
80-81	2.9859670744875614E-4	7.464917686218903E-5	0.0	1.5565659736361501	2.612721190176616E-4
82-83	2.9859670744875614E-4	7.464917686218903E-5	0.0	1.7424237617287854	2.612721190176616E-4
84-85	2.9859670744875614E-4	9.33114710777363E-5	0.0	1.9301851038314064	2.612721190176616E-4
86-87	2.9859670744875614E-4	1.1197376529328356E-4	0.0	2.1262138422715147	2.612721190176616E-4
88	2.9859670744875614E-4	1.1197376529328356E-4	0.0	2.288781087183147	2.612721190176616E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3945	0.0	48.190857	1
TACCTGG	3180	0.0	44.98089	2
GTACCTG	3570	0.0	40.598763	1
GTATCAA	7140	0.0	39.346527	1
TATAACG	295	0.0	36.684906	2
ACCTGGG	3935	0.0	35.274334	3
TCAACGC	8565	0.0	31.85969	4
ATCAACG	8580	0.0	31.697289	3
TATCAAC	9385	0.0	29.780619	2
CAACGCA	9195	0.0	29.778587	5
AACGCAG	9825	0.0	28.26995	6
GTACATG	16170	0.0	27.588533	1
TACATGG	16050	0.0	26.38451	2
TCTGTCG	665	0.0	26.148218	8
CATGGGT	6600	0.0	25.876385	4
ATAACGC	390	0.0	25.335896	3
CGCAGAG	11025	0.0	24.766212	8
ACATGGG	16465	0.0	24.662132	3
ACGCAGA	11270	0.0	24.520174	7
GTACACG	375	0.0	23.842545	1
>>END_MODULE