##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431400_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2679199
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6154503640827	34.0	31.0	34.0	31.0	34.0
2	32.699602381159444	34.0	31.0	34.0	31.0	34.0
3	32.803773441241205	34.0	31.0	34.0	31.0	34.0
4	36.28971121592685	37.0	37.0	37.0	35.0	37.0
5	36.20705255563323	37.0	37.0	37.0	35.0	37.0
6	36.254072579155185	37.0	37.0	37.0	35.0	37.0
7	36.20674947997517	37.0	37.0	37.0	35.0	37.0
8	36.21773821205517	37.0	37.0	37.0	35.0	37.0
9	38.00207263439558	39.0	39.0	39.0	35.0	39.0
10-11	37.97575301424045	39.0	38.5	39.0	35.0	39.0
12-13	37.95575916533262	39.0	38.0	39.0	35.0	39.0
14-15	39.382872268913204	41.0	39.0	41.0	36.0	41.0
16-17	39.42105998845177	41.0	39.0	41.0	36.0	41.0
18-19	39.49939683465095	41.0	39.5	41.0	36.0	41.0
20-21	39.46987233871019	41.0	39.0	41.0	36.0	41.0
22-23	39.365241812944845	41.0	39.0	41.0	36.0	41.0
24-25	39.29935812905275	41.0	39.0	41.0	35.0	41.0
26-27	39.16056907306997	41.0	39.0	41.0	35.0	41.0
28-29	39.05983878017273	41.0	39.0	41.0	35.0	41.0
30-31	38.829392665494424	41.0	38.5	41.0	35.0	41.0
32-33	38.53680503016014	40.5	38.0	41.0	35.0	41.0
34-35	38.35095843944403	40.0	38.0	41.0	35.0	41.0
36-37	38.181758988414074	40.0	38.0	41.0	34.0	41.0
38-39	38.026660766893386	40.0	38.0	41.0	34.0	41.0
40-41	37.87579646006138	40.0	38.0	41.0	34.0	41.0
42-43	37.68031769943181	40.0	37.0	41.0	33.0	41.0
44-45	37.394904036616914	40.0	37.0	41.0	33.0	41.0
46-47	36.97264443589297	40.0	37.0	41.0	31.5	41.0
48-49	36.5991576213637	40.0	36.5	41.0	30.5	41.0
50-51	36.415248550033056	40.0	36.0	41.0	30.0	41.0
52-53	36.34613591599579	40.0	36.0	41.0	30.0	41.0
54-55	36.192601967976245	40.0	35.5	41.0	30.0	41.0
56-57	36.033822795544495	40.0	35.0	41.0	29.5	41.0
58-59	35.84310534603813	39.5	35.0	41.0	29.0	41.0
60-61	35.672091733387475	39.0	35.0	41.0	29.0	41.0
62-63	35.48746304399188	39.0	35.0	41.0	28.5	41.0
64-65	35.24033395802253	38.5	35.0	41.0	28.0	41.0
66-67	34.96609621009861	37.5	35.0	41.0	28.0	41.0
68-69	34.66157870318703	37.0	35.0	40.0	28.0	41.0
70-71	34.35239394311509	37.0	35.0	40.0	27.5	41.0
72-73	34.03079707778332	36.0	35.0	39.0	26.0	41.0
74-75	33.70262697918295	36.0	34.0	39.0	26.0	41.0
76-77	33.3844902898217	35.0	34.0	38.5	26.0	40.5
78-79	33.08128287596405	35.0	34.0	37.0	26.0	39.5
80-81	32.833893077744506	35.0	34.0	37.0	26.0	39.0
82-83	32.6540415624222	35.0	34.0	36.5	26.0	39.0
84-85	32.41036817347274	35.0	34.0	36.0	26.0	37.5
86-87	32.185696919116495	35.0	34.0	36.0	25.5	37.0
88-89	31.967394172661308	35.0	34.0	35.5	25.0	37.0
90-91	31.797265526002363	35.0	34.0	35.0	25.0	36.0
92-93	31.638690705692262	35.0	34.0	35.0	24.5	36.0
94-95	31.4992018136764	35.0	34.0	35.0	24.0	36.0
96-97	31.357397864063103	35.0	34.0	35.0	23.0	36.0
98-99	31.24273038322275	35.0	34.0	35.0	20.5	36.0
100	31.138814250079967	35.0	34.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.018088796930875617
1101	2	0.029898975176479325
1101	3	-0.007234702619847155
1101	4	0.06990373468200772
1101	5	0.03185739706707835
1101	6	0.04996035939721111
1101	7	-0.007330440821156969
1101	8	0.03425971298708674
1101	9	0.08687471500513055
1101	10-11	0.07930039874511863
1101	12-13	0.03300727623756927
1101	14-15	0.05823230844715965
1101	16-17	0.0751467876520806
1101	18-19	0.031054975120525796
1101	20-21	0.07706304491927796
1101	22-23	0.061786554124296345
1101	24-25	0.05715155789064852
1101	26-27	0.08737952226997692
1101	28-29	0.0027674719687880156
1101	30-31	-0.04877362576651478
1101	32-33	-0.004116201746718673
1101	34-35	0.0019550069959990424
1101	36-37	-1.9267769519615285E-4
1101	38-39	0.07087265537804655
1101	40-41	0.09018738647590396
1101	42-43	0.13326519656732927
1101	44-45	0.17311652023684587
1101	46-47	0.1273036967827892
1101	48-49	0.2621164599209038
1101	50-51	0.13589557499652472
1101	52-53	0.12494514172966831
1101	54-55	0.05750014337454701
1101	56-57	0.11268639306572226
1101	58-59	0.17470285788900242
1101	60-61	0.21739991786217416
1101	62-63	0.17529983550483053
1101	64-65	0.10954270926591647
1101	66-67	0.14321885013112734
1101	68-69	0.1300178880866767
1101	70-71	0.1331418287509578
1101	72-73	0.06880797934821459
1101	74-75	0.0678383498346875
1101	76-77	0.08359623490630952
1101	78-79	0.07250138399875539
1101	80-81	0.09650867094096327
1101	82-83	0.12000077182707969
1101	84-85	0.1685464274755759
1101	86-87	0.17828043689551265
1101	88-89	0.16923750336200172
1101	90-91	0.16252373878555915
1101	92-93	0.1603816861204308
1101	94-95	0.10558679743279242
1101	96-97	0.1395888471726252
1101	98-99	0.20385701215088758
1101	100	0.2713883163903539
1102	1	-0.06158006637216573
1102	2	-0.04150578549995032
1102	3	-0.03040816281535541
1102	4	-0.0032829348117147106
1102	5	-0.04209561014587848
1102	6	-0.01776315978660392
1102	7	-0.0657644359989078
1102	8	0.007068330098292108
1102	9	0.003680797405074543
1102	10-11	-0.031053154074214717
1102	12-13	-0.09207744326893419
1102	14-15	-0.05903422854539997
1102	16-17	-0.03904486707613586
1102	18-19	-0.0761678535514747
1102	20-21	0.02495813899726329
1102	22-23	0.05702518321457006
1102	24-25	0.040868475826897566
1102	26-27	0.06988503275832869
1102	28-29	0.014181113488845654
1102	30-31	0.019001483380272077
1102	32-33	0.11305438358392905
1102	34-35	0.14214403460456992
1102	36-37	0.11434157548907109
1102	38-39	0.014378350683195151
1102	40-41	0.04211896996461917
1102	42-43	0.05243924409472811
1102	44-45	0.07695601819280284
1102	46-47	0.008739509231162401
1102	48-49	-0.002539908406490099
1102	50-51	0.022754357110564172
1102	52-53	0.04682705995787728
1102	54-55	0.030827140402173825
1102	56-57	0.09423554044236937
1102	58-59	0.11974941357865987
1102	60-61	0.02713794147904025
1102	62-63	0.02134174441282255
1102	64-65	-0.008326324794232676
1102	66-67	0.003742054559474184
1102	68-69	0.030250872044462085
1102	70-71	0.018961654206798073
1102	72-73	0.001306162361053964
1102	74-75	0.05655361235672984
1102	76-77	-0.002749666441211218
1102	78-79	0.0039254611478583
1102	80-81	-0.009824939523063847
1102	82-83	0.006209364503909853
1102	84-85	0.03739790309024471
1102	86-87	0.08260720488637929
1102	88-89	0.031700327407907736
1102	90-91	0.04322159508086898
1102	92-93	0.06420286538761388
1102	94-95	0.058786834963072465
1102	96-97	0.05584058549926141
1102	98-99	0.0898997090813971
1102	100	0.06884293281331111
1103	1	-0.03541584199075487
1103	2	-0.09554974136317895
1103	3	-0.04784964366177746
1103	4	0.007199032859752208
1103	5	0.025946301699910634
1103	6	0.0014993253807062956
1103	7	-0.04700729727751707
1103	8	-8.467728233512162E-4
1103	9	0.007817469229927099
1103	10-11	0.018010679334921065
1103	12-13	-0.01121842740689516
1103	14-15	-0.01681755833990195
1103	16-17	-0.03770366074037668
1103	18-19	-0.06104467588241391
1103	20-21	-0.10152722988772211
1103	22-23	-0.11438559013909355
1103	24-25	-0.14749087230570979
1103	26-27	-0.10397948224298403
1103	28-29	-0.11300696106598451
1103	30-31	-0.04532346127234632
1103	32-33	-0.10221483850514801
1103	34-35	-0.14572969597605123
1103	36-37	-0.11583684273157502
1103	38-39	-0.10976668079483431
1103	40-41	-0.11117674984937764
1103	42-43	0.008024246564879434
1103	44-45	0.012986432598680153
1103	46-47	0.03566516208570647
1103	48-49	0.04162564899310439
1103	50-51	-0.08781716722868538
1103	52-53	-0.015371634252211663
1103	54-55	5.397037013850081E-4
1103	56-57	-0.037934905674518404
1103	58-59	0.0025855780820762675
1103	60-61	-0.05125324066826664
1103	62-63	-0.02475091194745005
1103	64-65	-0.026430167936375426
1103	66-67	0.03729941310305662
1103	68-69	-0.024590041169275878
1103	70-71	0.014647373137329112
1103	72-73	-0.054113404161341805
1103	74-75	-0.03166230594386121
1103	76-77	0.04893371004337155
1103	78-79	0.018081452107281848
1103	80-81	0.04297849272814602
1103	82-83	0.002235507010340143
1103	84-85	-0.02655850413690075
1103	86-87	7.864493730593836E-4
1103	88-89	-0.024629706132611062
1103	90-91	-0.08607543989901245
1103	92-93	-0.07815871062699742
1103	94-95	-0.09531062155587477
1103	96-97	0.012461829322031548
1103	98-99	-0.05733768700542541
1103	100	-0.08811380763323484
1104	1	-0.15795140783062322
1104	2	-0.09519060245876432
1104	3	-0.14018516632557976
1104	4	-0.09938267524512412
1104	5	-0.06501522546795258
1104	6	-0.10076457073499512
1104	7	-0.1222026470755253
1104	8	-0.1508011942839076
1104	9	-0.13474780787650076
1104	10-11	-0.12813007299914148
1104	12-13	-0.11168745035879368
1104	14-15	-0.15522470919452758
1104	16-17	-0.13269685920941754
1104	18-19	-0.1325233794998013
1104	20-21	-0.08585831502657015
1104	22-23	-0.1302793518271912
1104	24-25	-0.0917630482603613
1104	26-27	-0.19319315900811063
1104	28-29	-0.2511183202729299
1104	30-31	-0.13687592149361905
1104	32-33	-0.19907440253091124
1104	34-35	-0.17766975242800953
1104	36-37	-0.2099870880507524
1104	38-39	-0.24137945335402122
1104	40-41	-0.2457463437411036
1104	42-43	-0.24141127496271508
1104	44-45	-0.2590591314300994
1104	46-47	-0.21286806315687556
1104	48-49	-0.2365165494790915
1104	50-51	-0.3261463506493527
1104	52-53	-0.3099542083424893
1104	54-55	-0.17847108798806488
1104	56-57	-0.1828945448135002
1104	58-59	-0.17270750650564537
1104	60-61	-0.27837896036634646
1104	62-63	-0.3797954480463517
1104	64-65	-0.2559155472800114
1104	66-67	-0.29298130798927247
1104	68-69	-0.36992779943340537
1104	70-71	-0.34411463050047075
1104	72-73	-0.35537283386765495
1104	74-75	-0.3262179463274748
1104	76-77	-0.33611038936672344
1104	78-79	-0.2843817808163678
1104	80-81	-0.27646382417022153
1104	82-83	-0.29628161276545484
1104	84-85	-0.37512570623333374
1104	86-87	-0.38804883448083416
1104	88-89	-0.3701492583609358
1104	90-91	-0.35735628515333673
1104	92-93	-0.41117421253234454
1104	94-95	-0.4242944521600407
1104	96-97	-0.4053934112106816
1104	98-99	-0.4494393758138564
1104	100	-0.4251722060694796
1105	1	-0.12941325156337058
1105	2	-0.13058072490634487
1105	3	-0.10315544571663793
1105	4	-0.04357789054486716
1105	5	-0.1302027273404036
1105	6	-0.009726815707196579
1105	7	-0.026339586356762368
1105	8	-0.04637661981172414
1105	9	-0.06237571231517336
1105	10-11	-0.07958290756864983
1105	12-13	-0.09283167026369199
1105	14-15	-0.14911771093644433
1105	16-17	-0.1486817710009376
1105	18-19	-0.14509019924378208
1105	20-21	-0.049285271791148944
1105	22-23	-0.10249552573614551
1105	24-25	-0.17301604418162952
1105	26-27	-0.10906254058576081
1105	28-29	-0.11762093795913842
1105	30-31	-0.1302915820300541
1105	32-33	-0.17593585936182876
1105	34-35	-0.12377353007072855
1105	36-37	-0.053693620443084455
1105	38-39	-0.01758319846960177
1105	40-41	-0.07449172681243965
1105	42-43	-0.09279853363055679
1105	44-45	-0.08871078068475668
1105	46-47	-0.06517188648740557
1105	48-49	-0.09996205688115367
1105	50-51	-0.17660139631574623
1105	52-53	-0.12527732915087597
1105	54-55	-0.09136233658104942
1105	56-57	-0.08994327882277275
1105	58-59	-0.11194453898142598
1105	60-61	-0.14137347462051508
1105	62-63	-0.1844587064959171
1105	64-65	-0.12924150939873158
1105	66-67	-0.10401678397696656
1105	68-69	-0.06944405177129909
1105	70-71	-0.09725292490109183
1105	72-73	-0.05377925787183102
1105	74-75	-0.11890200021554165
1105	76-77	-0.19161703977694344
1105	78-79	-0.1141861016838206
1105	80-81	-0.09235396320237044
1105	82-83	-0.1104812630986487
1105	84-85	-0.17068256774901158
1105	86-87	-0.1061617999720994
1105	88-89	-0.058559737558542935
1105	90-91	-0.03503260581963019
1105	92-93	-0.10585813748879502
1105	94-95	-0.07765927298254738
1105	96-97	-0.014516566579160894
1105	98-99	-0.11925220563268724
1105	100	-0.10756549777377344
1106	1	-0.18030119184957272
1106	2	-0.13244004775491902
1106	3	-0.1730469629883089
1106	4	-0.061386484529563745
1106	5	-0.146703942458835
1106	6	-0.18902554319954135
1106	7	-0.18221555933202893
1106	8	-0.14991982177829044
1106	9	-0.1860001578461734
1106	10-11	-0.13070522172182564
1106	12-13	-0.12066923208141844
1106	14-15	-0.1661026296902932
1106	16-17	-0.15745204757764242
1106	18-19	-0.16472839850662524
1106	20-21	-0.17595421443093073
1106	22-23	-0.09386597059587132
1106	24-25	-0.07692657862655494
1106	26-27	-0.14889065648920763
1106	28-29	-0.1276152676654121
1106	30-31	-0.16068365713742594
1106	32-33	-0.12193126459813186
1106	34-35	-0.12027080615434471
1106	36-37	-0.11606944114007689
1106	38-39	-0.1446392788269506
1106	40-41	-0.16610406881078177
1106	42-43	-0.18878094471351403
1106	44-45	-0.14898694820328728
1106	46-47	-0.28288037952300016
1106	48-49	-0.15448214466387356
1106	50-51	-0.22454609936276881
1106	52-53	-0.29829095235849934
1106	54-55	-0.31904000621593553
1106	56-57	-0.26803549885347877
1106	58-59	-0.17807665410586537
1106	60-61	-0.20868416010062418
1106	62-63	-0.24772799663055878
1106	64-65	-0.23892637495877267
1106	66-67	-0.20742926748015122
1106	68-69	-0.2800411107806724
1106	70-71	-0.24946026704446211
1106	72-73	-0.2053269217470728
1106	74-75	-0.20172878601491107
1106	76-77	-0.24301092331825203
1106	78-79	-0.2510725795288806
1106	80-81	-0.21453302768547644
1106	82-83	-0.19324307813131014
1106	84-85	-0.18272944925580248
1106	86-87	-0.22786437979388197
1106	88-89	-0.20545190771034427
1106	90-91	-0.2203987677235446
1106	92-93	-0.18916749390074727
1106	94-95	-0.2386647316923458
1106	96-97	-0.2327851613615053
1106	98-99	-0.2104807442378167
1106	100	-0.15913577678485424
1107	1	-0.28068201579034735
1107	2	-0.20643459977048195
1107	3	-0.21617312818080592
1107	4	-0.16174522540941894
1107	5	-0.27335788596477073
1107	6	-0.2400277098277428
1107	7	-0.18740022659628863
1107	8	-0.17368536312120142
1107	9	-0.12864957774593222
1107	10-11	-0.12520856124049118
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2216	32-33	0.11061830894862368
2216	34-35	0.11279576952363612
2216	36-37	0.11324911839516716
2216	38-39	0.11818119268799876
2216	40-41	0.14351909521668915
2216	42-43	0.15456334387938142
2216	44-45	0.14643590425746567
2216	46-47	0.10424291005372766
2216	48-49	0.177876660491755
2216	50-51	0.23790068539486953
2216	52-53	0.22771676838394228
2216	54-55	0.2463858860199437
2216	56-57	0.1728499266828294
2216	58-59	0.17735733771925766
2216	60-61	0.1387905712572035
2216	62-63	0.11138402593577723
2216	64-65	0.03103161027581791
2216	66-67	0.12200160622302292
2216	68-69	0.1285828857074165
2216	70-71	0.12217392608905442
2216	72-73	0.0988527741710854
2216	74-75	0.10771589861813169
2216	76-77	0.08672477171170101
2216	78-79	0.11775156037687395
2216	80-81	0.14016850822943638
2216	82-83	0.19251300302986607
2216	84-85	0.16797885312490735
2216	86-87	0.15449016026676787
2216	88-89	0.14767223345345215
2216	90-91	0.1141610105487949
2216	92-93	0.20645525036773904
2216	94-95	0.17940343291250116
2216	96-97	0.1562987166919605
2216	98-99	0.16508758989688133
2216	100	0.19282252060968474
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	22.0
3	144.0
4	53.0
5	120.0
6	375.0
7	899.0
8	1656.0
9	2616.0
10	3617.0
11	5217.0
12	8323.0
13	12179.0
14	11365.0
15	13415.0
16	17236.0
17	26138.0
18	29821.0
19	15307.0
20	9440.0
21	8322.0
22	8042.0
23	8194.0
24	8861.0
25	10235.0
26	12505.0
27	15728.0
28	19099.0
29	23955.0
30	30294.0
31	38182.0
32	47966.0
33	63050.0
34	90695.0
35	155384.0
36	235783.0
37	477257.0
38	803687.0
39	459683.0
40	4334.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.64544556329172	20.0082342713659	14.370035314796642	21.976284850545735
2	14.651380239001293	21.67894395992632	41.06257220336199	22.607103597710395
3	16.14051392878005	29.544242329233178	31.503397513777614	22.811846228209163
4	10.831483588938335	18.41065184034482	38.96492944346426	31.792935127252587
5	10.989868574883042	38.51356725264114	35.03670321865888	15.459860953816937
6	26.54789733797303	39.4849729340747	19.581412205662964	14.385717522289312
7	24.20760831875497	34.55006515006911	24.00904150830155	17.233285022874373
8	25.4764203778816	33.584627345710416	21.97492608798376	18.964026188424228
9	26.22538535785709	15.237992862043045	22.7907381238714	35.745883656228465
10-11	22.825926704212716	26.969870472480768	29.910114926140235	20.29408789716628
12-13	24.92661052799736	25.400110256834225	30.015482239281216	19.657796975887194
14-15	21.995775975538955	26.14063496974935	29.410064241227367	22.453524813484332
16-17	19.272495249513007	29.278470916120824	29.667038543982738	21.78199529038343
18-19	19.293836394887272	26.42812486586456	34.335126782485034	19.942911956763133
20-21	21.194487606183788	25.597781277165303	34.04250673428887	19.16522438236204
22-23	20.45776385321279	25.938713749413072	34.2010743513544	19.402448046019742
24-25	20.032114087872564	25.874216836530934	34.282815230527945	19.810853845068564
26-27	19.753127430766543	25.857962612664398	34.314846692813816	20.074063263755246
28-29	19.829157543118853	25.8148207660253	34.37456085198774	19.981460838868117
30-31	20.18370470787637	26.35691147066409	33.921396421531725	19.537987399927815
32-33	19.67911674055467	26.021553336997407	34.41011060338706	19.889219319060867
34-35	19.950439949029068	25.775238935582383	34.04095814605546	20.233362969333086
36-37	20.44572361269146	26.16894812444817	33.283106363291786	20.10222189956858
38-39	20.255272945386288	26.294807928495807	33.44237201687164	20.007547109246275
40-41	19.9907472189869	26.13666159735697	33.49344364509557	20.379147538560563
42-43	19.988712993904496	26.29616745676954	33.18497365058878	20.530145898737192
44-45	20.562284252013615	26.419002861492242	32.71725310181616	20.30145978467798
46-47	20.521918158968706	26.98481334311737	31.914803481942705	20.57846501597122
48-49	20.679738391324996	26.754815116868237	31.611912917791464	20.953533574015303
50-51	20.683329280493762	26.773468787132238	31.35259275046222	21.190609181911782
52-53	20.706596794541614	27.07630692973223	31.138221396098807	21.07887487962735
54-55	20.862845743802325	27.536835575352736	30.91603573420793	20.68428294663701
56-57	20.535354528812505	27.12035818950145	30.898715464903763	21.445571816782287
58-59	20.496311470897208	26.649713746850956	31.46410782833358	21.389866953918254
60-61	21.003629674986485	27.04510042399396	30.938756862911234	21.01251303810832
62-63	20.72182164151083	26.917984925159544	31.215534983647895	21.14465844968173
64-65	20.652026843553383	26.77670391039558	31.47356058546104	21.097708660589994
66-67	20.598618162897925	26.86927041673463	31.11643569485818	21.415675725509267
68-69	21.248802343692557	27.1117102140161	30.727055293930146	20.912432148361205
70-71	21.194906592052035	26.84047800294753	30.814946391416655	21.14966901358378
72-73	20.951521063513677	26.976204975040076	31.08613428401708	20.986139677429165
74-75	20.851715823498235	27.099709612642677	30.857613150292252	21.19096141356684
76-77	21.01089252408482	27.146340088766063	30.554868155407426	21.28789923174169
78-79	21.080030031627267	27.22703309283024	30.721858273188897	20.971078602353597
80-81	20.83562804233624	27.096129193718728	30.825735054935848	21.242507709009185
82-83	20.62899985741789	27.04986044122818	31.02394483640009	21.297194864953838
84-85	20.807695681303688	27.13584269725829	30.588448266530804	21.46801335490722
86-87	20.895023510277056	27.149363743504228	30.548112785876853	21.407499960341863
88-89	20.64222597629262	27.050787446541204	30.733575299178924	21.573411277987255
90-91	20.517145428302584	27.009139774310615	30.577167771051393	21.896547026335405
92-93	20.671112958289374	27.292214373788216	30.41425460913797	21.622418058784437
94-95	20.578016515877344	27.044007316419076	30.679915250172673	21.69806091753091
96-97	20.500867051807372	27.246720468917147	30.501244030854057	21.751168448421428
98-99	20.387430529376417	27.163060477233792	30.492406286974795	21.957102706415
100	20.51079636412362	27.27090175422488	30.393787075118627	21.82451480653287
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3037.0
1	1840.5
2	7332.5
3	11068.5
4	5006.0
5	2105.0
6	3570.0
7	9177.5
8	15486.5
9	15888.0
10	9961.0
11	4590.5
12	3694.5
13	3949.0
14	4306.5
15	4613.0
16	4932.0
17	5417.0
18	5904.5
19	6440.5
20	7426.5
21	8899.5
22	10828.0
23	14336.5
24	18274.0
25	21473.5
26	26006.5
27	32538.5
28	41613.0
29	49863.5
30	53614.5
31	57973.5
32	63000.5
33	67630.0
34	72973.0
35	77421.0
36	79399.5
37	80243.0
38	81208.5
39	81372.0
40	82351.0
41	82001.5
42	80250.0
43	79868.0
44	79894.5
45	78805.5
46	78457.5
47	78484.0
48	76662.5
49	75365.0
50	76239.0
51	74556.5
52	68859.5
53	64518.0
54	62363.5
55	60429.0
56	56606.0
57	51372.0
58	47827.5
59	44867.0
60	40436.5
61	34698.0
62	29334.0
63	23522.0
64	17747.5
65	14162.5
66	11150.0
67	8322.5
68	6414.0
69	4927.5
70	3693.5
71	2859.0
72	2233.0
73	1788.0
74	1552.5
75	1191.5
76	784.5
77	614.0
78	464.0
79	285.0
80	150.0
81	79.0
82	47.5
83	27.5
84	12.5
85	7.5
86	6.5
87	3.5
88	2.5
89	2.5
90	1.5
91	0.0
92	0.0
93	0.0
94	0.5
95	1.5
96	1.5
97	0.5
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005673337441526366
2	0.0050014948497666654
3	0.0012690360066572137
4	0.0
5	0.04090774892047959
6	0.0
7	0.0
8	0.0
9	3.732458843109452E-5
10-11	0.0
12-13	0.0
14-15	1.866229421554726E-5
16-17	0.0
18-19	1.4929835372437807E-4
20-21	0.0
22-23	3.732458843109452E-5
24-25	3.732458843109452E-5
26-27	1.1197376529328356E-4
28-29	2.0528523637101985E-4
30-31	2.9859670744875614E-4
32-33	0.0011757245355794772
34-35	9.331147107773629E-4
36-37	0.0010264261818550993
38-39	0.0010264261818550993
40-41	1.6796064793992533E-4
42-43	4.4789506117313423E-4
44-45	9.33114710777363E-5
46-47	1.866229421554726E-4
48-49	2.4260982480211435E-4
50-51	2.7993441323320885E-4
52-53	7.091671801907958E-4
54-55	0.0011010753587172883
56-57	0.002183488423219029
58-59	5.412065322508705E-4
60-61	0.0012503737124416664
62-63	0.001231711418226119
64-65	0.0016982687736148006
66-67	4.4789506117313423E-4
68-69	2.9859670744875614E-4
70-71	3.919081785264925E-4
72-73	1.6796064793992533E-4
74-75	7.091671801907958E-4
76-77	8.211409454840794E-4
78-79	8.771278281307211E-4
80-81	0.0010637507702861938
82-83	0.0015303081256748752
84-85	0.0012690360066572137
86-87	0.0019222163042013677
88-89	0.001231711418226119
90-91	0.0011197376529328354
92-93	0.0013996720661660445
94-95	7.27829474406343E-4
96-97	1.866229421554726E-4
98-99	3.3592129587985066E-4
100	3.732458843109452E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2679199.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.551208000764746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.67420546673637	43.55141939108753
2	15.062982727552843	17.337857041306407
3	4.441145820684655	7.667799206638488
4	1.744396942885543	4.015686051836161
5	0.8176000820159433	2.352693619077093
6	0.4948903217964513	1.708892150836378
7	0.33673320360593995	1.356558184904252
8	0.22351534529496622	1.0290862502746685
9	0.17453897624210396	0.9040436033354873
>10	0.9333204265963266	9.79608328285755
>50	0.06275644912033618	2.490249851400654
>100	0.031247304485188417	3.205215176209081
>500	0.0016912257942616438	0.6792656642264452
>1k	6.504714593232047E-4	0.7678378638092448
>5k	6.504714593232047E-5	0.2624597173792899
>10k+	2.601885837292819E-4	2.8748529448213618
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25319	0.9450212544868821	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19439	0.7255526745120464	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17224	0.642878711137172	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14813	0.5528891284298031	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7011	0.2616826894904037	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4096	0.15288151421376314	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3978	0.148477212778894	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3604	0.13451781670566462	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.732458843109452E-5	0.0
4	0.0	0.0	0.0	3.732458843109452E-5	0.0
5	0.0	0.0	0.0	3.732458843109452E-5	0.0
6	0.0	0.0	0.0	3.732458843109452E-5	0.0
7	0.0	0.0	0.0	3.732458843109452E-5	0.0
8	0.0	0.0	0.0	3.732458843109452E-5	0.0
9	1.4929835372437807E-4	0.0	0.0	7.464917686218903E-5	0.0
10-11	1.4929835372437807E-4	0.0	0.0	7.464917686218903E-5	0.0
12-13	1.4929835372437807E-4	0.0	0.0	7.464917686218903E-5	0.0
14-15	1.4929835372437807E-4	0.0	0.0	1.4929835372437807E-4	0.0
16-17	1.4929835372437807E-4	0.0	0.0	1.4929835372437807E-4	3.732458843109452E-5
18-19	2.0528523637101985E-4	0.0	0.0	1.4929835372437807E-4	3.732458843109452E-5
20-21	2.2394753058656712E-4	0.0	0.0	2.612721190176616E-4	3.732458843109452E-5
22-23	2.2394753058656712E-4	0.0	0.0	6.345180033286069E-4	3.732458843109452E-5
24-25	2.2394753058656712E-4	0.0	0.0	0.0013250228893038553	3.732458843109452E-5
26-27	2.2394753058656712E-4	0.0	0.0	0.0018289048331236314	3.732458843109452E-5
28-29	2.2394753058656712E-4	0.0	0.0	0.0030232916629186557	3.732458843109452E-5
30-31	2.2394753058656712E-4	0.0	0.0	0.005430727616724252	3.732458843109452E-5
32-33	2.2394753058656712E-4	0.0	0.0	0.00940579628463582	3.732458843109452E-5
34-35	2.612721190176616E-4	0.0	0.0	0.01604957302537064	3.732458843109452E-5
36-37	2.612721190176616E-4	0.0	0.0	0.026239185667059448	3.732458843109452E-5
38-39	2.612721190176616E-4	0.0	0.0	0.04230742098664564	3.732458843109452E-5
40-41	2.612721190176616E-4	0.0	0.0	0.061380285674934934	3.732458843109452E-5
42-43	2.612721190176616E-4	0.0	0.0	0.0823007174905634	3.732458843109452E-5
44-45	2.612721190176616E-4	3.732458843109452E-5	0.0	0.10626310326332608	3.732458843109452E-5
46-47	2.612721190176616E-4	3.732458843109452E-5	0.0	0.1345364789998802	3.732458843109452E-5
48-49	2.612721190176616E-4	3.732458843109452E-5	0.0	0.16723281846551896	3.732458843109452E-5
50-51	2.612721190176616E-4	3.732458843109452E-5	0.0	0.20476269213298454	3.732458843109452E-5
52-53	2.612721190176616E-4	3.732458843109452E-5	0.0	0.24378554933769386	3.732458843109452E-5
54-55	2.612721190176616E-4	3.732458843109452E-5	0.0	0.28708207191776347	3.732458843109452E-5
56-57	2.612721190176616E-4	3.732458843109452E-5	0.0	0.3359959450567128	3.732458843109452E-5
58-59	3.172590016643034E-4	3.732458843109452E-5	0.0	0.3936624341827539	1.1197376529328356E-4
60-61	3.732458843109452E-4	3.732458843109452E-5	0.0	0.45504271985768885	1.1197376529328356E-4
62-63	4.105704727420397E-4	3.732458843109452E-5	0.0	0.5201927889641643	1.4929835372437807E-4
64-65	4.105704727420397E-4	3.732458843109452E-5	0.0	0.5890753169137493	1.4929835372437807E-4
66-67	4.105704727420397E-4	3.732458843109452E-5	0.0	0.6721411884671501	1.4929835372437807E-4
68-69	4.105704727420397E-4	3.732458843109452E-5	0.0	0.7665350726093881	1.4929835372437807E-4
70-71	4.105704727420397E-4	3.732458843109452E-5	0.0	0.8751682872380887	1.4929835372437807E-4
72-73	4.105704727420397E-4	3.732458843109452E-5	0.0	1.0003736191301953	1.4929835372437807E-4
74-75	5.225442380353232E-4	3.732458843109452E-5	0.0	1.1256349379049484	1.4929835372437807E-4
76-77	5.225442380353232E-4	3.732458843109452E-5	0.0	1.25748404653779	1.4929835372437807E-4
78-79	5.225442380353232E-4	3.732458843109452E-5	0.0	1.4074169182654965	1.4929835372437807E-4
80-81	5.225442380353232E-4	3.732458843109452E-5	0.0	1.5833090412470294	1.4929835372437807E-4
82-83	5.225442380353232E-4	3.732458843109452E-5	0.0	1.7726940029464031	1.4929835372437807E-4
84-85	5.225442380353232E-4	3.732458843109452E-5	0.0	1.9643930891285044	1.4929835372437807E-4
86-87	5.225442380353232E-4	3.732458843109452E-5	0.0	2.165068738828284	1.4929835372437807E-4
88	5.225442380353232E-4	3.732458843109452E-5	0.0	2.3312564688177324	1.4929835372437807E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	360	0.0	62.7106	2
GGTATCA	8015	0.0	61.264282	1
TACCTGG	2995	0.0	48.681858	2
ATAACGC	495	0.0	47.504486	3
GTATCAA	16640	0.0	47.48608	1
GTACCTG	3125	0.0	46.95858	1
ACCTGGG	3530	0.0	40.101593	3
ATCAACG	21895	0.0	35.913784	3
TATCAAC	21985	0.0	35.769436	2
TCAACGC	22125	0.0	35.51786	4
CAACGCA	22790	0.0	34.564007	5
AACGCAG	23725	0.0	33.458107	6
TAACGCA	775	0.0	32.767677	4
ACGCAGA	26415	0.0	29.944113	7
CGCAGAG	26490	0.0	29.859331	8
TCTGTCG	605	0.0	28.742483	8
GCAGAGT	28110	0.0	28.005285	9
GTACATG	15165	0.0	26.734694	1
TATCACG	320	0.0	26.456036	2
GTCTCGC	720	0.0	25.47666	1
>>END_MODULE