FastQCFastQC Report
Wed 25 May 2016
SRR2431399_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431399_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2610400
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA293381.1238890591480233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43100.046.9393231
GTATCAA77250.038.613251
TCAACGC91650.030.9525994
ATCAACG91700.030.8861963
TATCAAC97550.029.950332
CAACGCA99700.028.406235
AACGCAG104050.027.324936
GTACATG173600.026.7493381
TACATGG173000.025.6722052
ACGCAGA115600.024.3102347
CATGGGT68800.024.2062264
CGCAGAG115350.024.0369958
ACATGGG179950.023.5041793
TACCTGG17500.022.5828382
GCAGAGT126500.021.1381749
GTGGTAT22200.020.769151
ATGGGTG37200.020.2343545
GTACCTG20500.020.1964231
TGGTATC22700.019.6895142
ATGGGTA28550.019.2793355
Produced by FastQC (version 0.11.5)