##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431399_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2610400
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.788424379405456	31.0	31.0	34.0	25.0	34.0
2	29.949444529574013	31.0	31.0	34.0	25.0	34.0
3	30.10176601287159	31.0	31.0	34.0	25.0	34.0
4	33.24935795280417	37.0	35.0	37.0	25.0	37.0
5	33.43505516395955	37.0	35.0	37.0	27.0	37.0
6	33.4340051333129	37.0	35.0	37.0	27.0	37.0
7	33.335315660435185	37.0	35.0	37.0	27.0	37.0
8	33.28167828685259	37.0	35.0	37.0	26.0	37.0
9	34.631995862703036	39.0	35.0	39.0	24.0	39.0
10-11	34.64970655838186	39.0	35.0	39.0	25.0	39.0
12-13	34.56094276739197	39.0	35.0	39.0	24.0	39.0
14-15	35.76858987128409	40.0	37.0	41.0	24.5	41.0
16-17	35.76502011186025	40.0	36.5	41.0	25.0	41.0
18-19	35.75034669016243	40.0	36.5	41.0	25.0	41.0
20-21	35.800217208090714	40.0	36.5	41.0	25.0	41.0
22-23	35.76609408519767	40.0	36.0	41.0	25.0	41.0
24-25	35.81063496015936	40.0	36.0	41.0	25.5	41.0
26-27	35.84363277658596	40.0	36.0	41.0	26.5	41.0
28-29	35.80648253141281	40.0	36.0	41.0	27.0	41.0
30-31	35.72582037235672	40.0	36.0	41.0	26.0	41.0
32-33	35.664994636837264	40.0	36.0	41.0	26.0	41.0
34-35	35.58148387220349	40.0	35.5	41.0	25.5	41.0
36-37	35.432884615384616	40.0	35.0	41.0	25.0	41.0
38-39	35.29496035090408	40.0	35.0	41.0	25.0	41.0
40-41	35.21053018694453	40.0	35.0	41.0	25.0	41.0
42-43	35.02059090560833	40.0	35.0	41.0	24.0	41.0
44-45	34.844941771376035	40.0	35.0	41.0	24.0	41.0
46-47	34.69240920931658	39.0	34.0	41.0	23.0	41.0
48-49	34.532367453263866	39.0	34.0	41.0	23.0	41.0
50-51	34.37463281489427	39.0	34.0	41.0	22.5	41.0
52-53	34.18537886913883	39.0	33.5	41.0	21.5	41.0
54-55	33.99268368832363	38.5	33.0	40.5	20.0	41.0
56-57	33.781882853202575	38.0	33.0	40.0	18.5	41.0
58-59	33.53687250996016	38.0	33.0	40.0	17.5	41.0
60-61	33.275651815813674	37.5	32.5	40.0	14.5	41.0
62-63	33.1642550950046	37.0	32.5	40.0	13.5	41.0
64-65	32.907320908673	37.0	32.0	40.0	10.0	41.0
66-67	32.63651547655532	36.0	32.0	40.0	8.0	41.0
68-69	32.33962074777812	36.0	32.0	39.0	6.5	41.0
70-71	32.01616763714373	35.5	31.0	39.0	2.0	41.0
72-73	31.68149115078149	35.0	31.0	39.0	2.0	40.5
74-75	31.40086749157217	35.0	31.0	38.0	2.0	40.0
76-77	31.15944108182654	35.0	31.0	37.0	2.0	39.5
78-79	30.82941541526203	35.0	31.0	37.0	2.0	39.0
80-81	30.519780110327922	35.0	31.0	36.0	2.0	39.0
82-83	30.21382470119522	35.0	30.0	36.0	2.0	38.0
84-85	29.929984868219428	35.0	30.0	35.5	2.0	37.0
86-87	29.665488813974868	35.0	30.0	35.0	2.0	37.0
88-89	29.41071330064358	34.5	30.0	35.0	2.0	36.5
90-91	29.18922521452651	34.0	30.0	35.0	2.0	36.0
92-93	28.960224869751762	34.0	29.0	35.0	2.0	36.0
94-95	28.73461864082133	34.0	29.0	35.0	2.0	36.0
96-97	28.528817997241802	34.0	29.0	35.0	2.0	35.0
98-99	28.293557500766166	34.0	29.0	35.0	2.0	35.0
100	28.103286086423537	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.270117788117922
1101	2	0.3489319414301839
1101	3	0.33199373983151403
1101	4	0.37278044204325056
1101	5	0.4021332082543907
1101	6	0.48859581450849987
1101	7	0.3833135556139098
1101	8	0.30435628193926334
1101	9	0.3813068984772059
1101	10-11	0.38861413176674375
1101	12-13	0.1737890864514
1101	14-15	0.3174170381162895
1101	16-17	0.23012728626123646
1101	18-19	0.16674246744697285
1101	20-21	0.2383578172765013
1101	22-23	0.28952281675960734
1101	24-25	0.23616362408533575
1101	26-27	0.2295995431457598
1101	28-29	0.25351053550470226
1101	30-31	0.2577612897151127
1101	32-33	0.2229037024148397
1101	34-35	0.21772697172772126
1101	36-37	0.15905172236084297
1101	38-39	0.17335874303395116
1101	40-41	0.11265320688494285
1101	42-43	0.2015103025680176
1101	44-45	0.11698528469121783
1101	46-47	0.18415701634627624
1101	48-49	0.19302264484454668
1101	50-51	0.11282980107991847
1101	52-53	0.1029576121433351
1101	54-55	0.08915159061373146
1101	56-57	-0.08353291657658701
1101	58-59	-0.08595715300948115
1101	60-61	0.07868374419189905
1101	62-63	-0.05740556390225038
1101	64-65	-0.01743005295982414
1101	66-67	0.047879058831235
1101	68-69	-0.02617912980544901
1101	70-71	-0.06498139165881156
1101	72-73	-0.05136799082703192
1101	74-75	-0.0033054303901778326
1101	76-77	0.05837368198559645
1101	78-79	-5.725132841263303E-4
1101	80-81	-0.1266337106351365
1101	82-83	-0.10817434200384923
1101	84-85	-0.05017319226208272
1101	86-87	-0.08002581022091348
1101	88-89	-0.14767262485563037
1101	90-91	-0.14310368288289155
1101	92-93	-0.05308599670854974
1101	94-95	-0.07623981047591499
1101	96-97	-0.14930248712224525
1101	98-99	-0.19999134305740895
1101	100	-0.19047170496788723
1102	1	-0.022641554510830275
1102	2	0.0271500801592488
1102	3	0.0764103452899434
1102	4	0.047274895775963444
1102	5	0.09953190044555527
1102	6	0.09232977929764274
1102	7	0.026172259168340872
1102	8	0.09873725841275416
1102	9	0.0733709192509906
1102	10-11	0.06173603894160351
1102	12-13	0.02356045817182917
1102	14-15	0.08167055695109582
1102	16-17	-0.01154172896582395
1102	18-19	0.030485894917177347
1102	20-21	0.025070879889668163
1102	22-23	0.06959688121002472
1102	24-25	0.019560690515476153
1102	26-27	0.0652117488528674
1102	28-29	-0.049991294638296324
1102	30-31	0.06735011673179514
1102	32-33	0.10242373320149767
1102	34-35	0.1161930698797633
1102	36-37	0.08513491390770866
1102	38-39	0.07655840137303471
1102	40-41	0.11936403180501998
1102	42-43	0.09957745181153399
1102	44-45	0.03068070164468395
1102	46-47	0.02073435725520767
1102	48-49	0.01227298575285829
1102	50-51	-0.02775129021927114
1102	52-53	0.07657454475166503
1102	54-55	-0.019932479154846305
1102	56-57	0.0031636789460947057
1102	58-59	-0.003823030569101604
1102	60-61	0.0420350343301763
1102	62-63	0.04596226130353642
1102	64-65	0.10302380058558924
1102	66-67	0.09197226006622827
1102	68-69	-0.0304305732596859
1102	70-71	0.06135767794225444
1102	72-73	0.007496551261265694
1102	74-75	0.024479212528873973
1102	76-77	0.08643789781185873
1102	78-79	-0.031061359049985526
1102	80-81	-0.027756739847298206
1102	82-83	0.010301171661513564
1102	84-85	0.06692235286277182
1102	86-87	0.009603474231287379
1102	88-89	-0.01628324288273575
1102	90-91	0.017276275461057367
1102	92-93	0.01346726853722302
1102	94-95	0.06868258575904562
1102	96-97	0.037985036948047224
1102	98-99	0.023880347371278532
1102	100	-0.02071084610437879
1103	1	-0.20898020931323913
1103	2	-0.14400930220138974
1103	3	-0.11437740660746343
1103	4	-0.06604622434966245
1103	5	-0.06194415294361022
1103	6	-0.049256192960925205
1103	7	-0.17115828257465893
1103	8	-0.13994371515437365
1103	9	-0.1166316978649462
1103	10-11	-0.005523840671067148
1103	12-13	-0.03702577579833388
1103	14-15	-0.04041790156762204
1103	16-17	-0.035750051394508375
1103	18-19	-0.019501019503131545
1103	20-21	-0.04729237580256296
1103	22-23	-0.044771085290882695
1103	24-25	0.046647840476218505
1103	26-27	-0.04091230244259947
1103	28-29	-0.05430953593638321
1103	30-31	-0.012733630978225108
1103	32-33	-0.05604263685869171
1103	34-35	-0.14185456163031773
1103	36-37	-0.11939269666543595
1103	38-39	-0.07693544840451239
1103	40-41	-0.021044238281341165
1103	42-43	0.005753845425772397
1103	44-45	-0.1767257364605257
1103	46-47	-0.24529547786649175
1103	48-49	-0.23530618200427256
1103	50-51	-0.19459243128182635
1103	52-53	-0.3021221315110978
1103	54-55	-0.26581052155211893
1103	56-57	-0.23447044824574448
1103	58-59	-0.2699837214877121
1103	60-61	-0.3305114194777232
1103	62-63	-0.27463444113038094
1103	64-65	-0.30132061187187986
1103	66-67	-0.2438037148094594
1103	68-69	-0.28481424245622833
1103	70-71	-0.26486582375887124
1103	72-73	-0.2904620983069144
1103	74-75	-0.28172335224474665
1103	76-77	-0.29435247120148844
1103	78-79	-0.31253217820727386
1103	80-81	-0.29797396047017344
1103	82-83	-0.36433897489698097
1103	84-85	-0.3749776733083898
1103	86-87	-0.3511659578545512
1103	88-89	-0.42808643576417893
1103	90-91	-0.48821966800923633
1103	92-93	-0.4365458170424681
1103	94-95	-0.44335614755684105
1103	96-97	-0.4133366066007653
1103	98-99	-0.5758317698755171
1103	100	-0.5574813250365942
1104	1	-0.23847862844862178
1104	2	-0.20269344878079565
1104	3	-0.19516029028395465
1104	4	-0.0707573770337433
1104	5	-0.0423957279052658
1104	6	-0.12142055059381107
1104	7	-0.1718188910720002
1104	8	-0.18877473670195855
1104	9	-0.09356234374219241
1104	10-11	-0.11058921215430217
1104	12-13	-0.055211807609367725
1104	14-15	-0.1768702605632697
1104	16-17	-0.11804479423878433
1104	18-19	-0.23682793691207138
1104	20-21	-0.3168338177371197
1104	22-23	-0.17468123923288914
1104	24-25	-0.2583697294256808
1104	26-27	-0.18058490426588492
1104	28-29	-0.17776025008430452
1104	30-31	-0.1675059391396374
1104	32-33	-0.2977788731267452
1104	34-35	-0.2130465134621673
1104	36-37	-0.30669168303523975
1104	38-39	-0.15058259760571246
1104	40-41	-0.20510220450982075
1104	42-43	-0.2986460465872369
1104	44-45	-0.18227407040892274
1104	46-47	-0.36331536684683385
1104	48-49	-0.3037432477962554
1104	50-51	-0.44972480616180377
1104	52-53	-0.559613041092355
1104	54-55	-0.39874556341359835
1104	56-57	-0.39078461196162095
1104	58-59	-0.5055134920758917
1104	60-61	-0.48256798825985214
1104	62-63	-0.4239275239911322
1104	64-65	-0.45068627773249403
1104	66-67	-0.39529269141255696
1104	68-69	-0.40880864111261417
1104	70-71	-0.4211142499805547
1104	72-73	-0.42573782151566775
1104	74-75	-0.4744316640783772
1104	76-77	-0.4160297177863761
1104	78-79	-0.3990562051753983
1104	80-81	-0.39014429123675143
1104	82-83	-0.44530004811815616
1104	84-85	-0.41838437119881533
1104	86-87	-0.35699753750004604
1104	88-89	-0.39545071105835916
1104	90-91	-0.40044153752165457
1104	92-93	-0.4687597347604502
1104	94-95	-0.5012354140268549
1104	96-97	-0.4939512421955783
1104	98-99	-0.45050710714910736
1104	100	-0.3403120950548484
1105	1	-0.23724377422892573
1105	2	-0.22762537732284827
1105	3	-0.29591081162299204
1105	4	-0.12781334466104965
1105	5	-0.2756947090722761
1105	6	-0.17067119973270906
1105	7	-0.2977812348914526
1105	8	-0.28065065351963625
1105	9	-0.2904945074686438
1105	10-11	-0.3387518367251374
1105	12-13	-0.21908412833774094
1105	14-15	-0.3138381252277185
1105	16-17	-0.32095864461305723
1105	18-19	-0.2574921123661724
1105	20-21	-0.2385396981365986
1105	22-23	-0.2818774872794876
1105	24-25	-0.42897995401317246
1105	26-27	-0.38191956214470935
1105	28-29	-0.3190919559089025
1105	30-31	-0.28732725479493837
1105	32-33	-0.28508262412743335
1105	34-35	-0.3941166596603054
1105	36-37	-0.360817610945233
1105	38-39	-0.3332135965433025
1105	40-41	-0.45445361002244056
1105	42-43	-0.4802390289459595
1105	44-45	-0.5469432416261597
1105	46-47	-0.5039438672223397
1105	48-49	-0.5605098011443275
1105	50-51	-0.5484245753054466
1105	52-53	-0.5623007850723525
1105	54-55	-0.5291875712677765
1105	56-57	-0.6382178967609491
1105	58-59	-0.5404285085728233
1105	60-61	-0.6476792122449595
1105	62-63	-0.5309841653237513
1105	64-65	-0.5203160024823603
1105	66-67	-0.5463255156968714
1105	68-69	-0.5184510519378449
1105	70-71	-0.4357708113395944
1105	72-73	-0.4874176533370438
1105	74-75	-0.5040342419063109
1105	76-77	-0.441432151237219
1105	78-79	-0.4856866795755508
1105	80-81	-0.4776519095509748
1105	82-83	-0.4019737185981356
1105	84-85	-0.42951466363047786
1105	86-87	-0.4572066787693849
1105	88-89	-0.44261559574042053
1105	90-91	-0.5423019318964997
1105	92-93	-0.46932659994562087
1105	94-95	-0.48397903063490233
1105	96-97	-0.5185788124494337
1105	98-99	-0.6162738328024169
1105	100	-0.6295784993711351
1106	1	0.024205690924443246
1106	2	-0.008732185767840406
1106	3	0.07758182260838353
1106	4	0.04978029901596415
1106	5	0.06227374103441008
1106	6	0.03029362293735005
1106	7	0.1354861129485343
1106	8	0.1486080424966758
1106	9	0.12381860671781908
1106	10-11	0.06884823557200548
1106	12-13	0.07558878995787666
1106	14-15	0.13513144177724712
1106	16-17	0.08824391518271568
1106	18-19	0.05036255579852167
1106	20-21	0.005060150383656037
1106	22-23	0.21174857888829735
1106	24-25	0.1998376764278973
1106	26-27	0.06812933111967112
1106	28-29	0.08430908869221554
1106	30-31	0.16063922236756412
1106	32-33	0.1060718991119316
1106	34-35	0.20258597461741346
1106	36-37	0.12322345542447977
1106	38-39	0.11638009612629219
1106	40-41	0.09762476750241689
1106	42-43	0.09170103724697753
1106	44-45	0.09932243185951961
1106	46-47	0.06812172588904275
1106	48-49	0.13475970104477852
1106	50-51	0.046562921657347545
1106	52-53	0.013081605275317543
1106	54-55	0.15082038009451537
1106	56-57	0.0550150040032662
1106	58-59	-0.022104723274175342
1106	60-61	0.003972764373664006
1106	62-63	0.015647926268520962
1106	64-65	0.03717257860664347
1106	66-67	0.12479869173239422
1106	68-69	0.053722655866472735
1106	70-71	0.03689199462399273
1106	72-73	0.0648923134075936
1106	74-75	0.12501736117704354
1106	76-77	0.07133974927078057
1106	78-79	0.1491306542618318
1106	80-81	0.15999683090954164
1106	82-83	0.0953700232295418
1106	84-85	0.11297325208640885
1106	86-87	0.04120377704030531
1106	88-89	-0.07761284487362019
1106	90-91	-0.01132501997786406
1106	92-93	-0.03498548716537542
1106	94-95	-0.05263291995008146
1106	96-97	-0.09483098649488753
1106	98-99	-0.018812310179434633
1106	100	0.05308064539066493
1107	1	-0.3268041655345755
1107	2	-0.3745836011917554
1107	3	-0.2338185875087042
1107	4	-0.1604924515958004
1107	5	-0.16147762791823084
1107	6	-0.27225482254990396
1107	7	-0.20462151841405074
1107	8	-0.19027833687137985
1107	9	-0.21922776381094877
1107	10-11	-0.19691738673679993
1107	12-13	-0.1600621490542693
1107	14-15	-0.14509113286113973
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2216	94-95	0.1056707004680959
2216	96-97	0.018612953351276218
2216	98-99	-0.05095444604207344
2216	100	-0.09696072928517196
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	91589.0
3	18070.0
4	14495.0
5	13457.0
6	8936.0
7	6836.0
8	6984.0
9	7300.0
10	7690.0
11	8253.0
12	9066.0
13	9869.0
14	10282.0
15	10100.0
16	10023.0
17	10299.0
18	9897.0
19	9575.0
20	9256.0
21	9883.0
22	11791.0
23	18151.0
24	28839.0
25	35422.0
26	37809.0
27	30159.0
28	29289.0
29	34240.0
30	40229.0
31	47091.0
32	60253.0
33	79490.0
34	111071.0
35	174106.0
36	279062.0
37	462904.0
38	600715.0
39	247145.0
40	774.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.34770685822327	22.144504790897813	13.893123970836713	21.614664380042203
2	14.961947589630281	23.22941077378415	39.38836021797299	22.420281418612582
3	17.183336640195144	30.363592334169837	29.553635059847807	22.89943596578722
4	11.689463408045043	20.203129173177466	38.04245550913149	30.06495190964601
5	12.009376946574202	41.82432118651842	33.3904040584309	12.775897808476481
6	27.14154056590464	40.7453567113003	18.66688697241629	13.446215750378762
7	24.898158222214043	36.474499493570534	22.224394642351918	16.40294764186351
8	24.317119926770005	37.78190999809489	20.279312027482714	17.621658047652396
9	25.340053853598704	19.772971626244527	20.427877382446795	34.459097137709975
10-11	22.432419999424972	30.601113634838946	27.88367211983554	19.08279424590054
12-13	23.24107340006624	28.99488922827807	27.335191795777668	20.428845575878015
14-15	21.29338098716417	30.06720462825086	25.074822481685622	23.564591902899345
16-17	20.394132855968245	32.83889119947561	25.898902354179086	20.86807359037706
18-19	20.60775474678419	31.64787937903911	27.602007870401163	20.142358003775538
20-21	22.531735777240712	30.415265297469784	26.865519733789206	20.187479191500298
22-23	22.12894050359478	30.484715244887674	26.903430708279586	20.482913543237967
24-25	21.47391211513331	30.621605780432354	26.99860043248208	20.90588167195226
26-27	21.069313428693334	30.68089770222854	27.11306305252953	21.1367258165486
28-29	21.164761891253573	30.548999298096042	27.29248694424138	20.993751866409006
30-31	21.360848874415634	30.792935911760427	26.96947792966006	20.87673728416388
32-33	21.08043777359412	30.69893270739484	27.219584115852292	21.00104540315875
34-35	21.443616556974185	30.282120869952067	27.234333002182602	21.039929570891143
36-37	21.42550020367575	30.479591690029473	26.841185632473103	21.253722473821675
38-39	21.246077613885774	30.550542952164623	27.111417809597743	21.09196162435186
40-41	21.232486135446237	30.264759163217896	27.264709901954753	21.238044799381107
42-43	21.162428772887704	30.464358506012818	27.223185856687227	21.150026864412254
44-45	21.269509290366486	30.32507895733189	27.207464882558458	21.197946869743166
46-47	21.33011941957868	30.349709597653778	27.086833176790815	21.233337805976728
48-49	21.231846552570236	30.305464820267453	27.102866979511713	21.359821647650595
50-51	21.211249316867743	30.265725268407955	27.077378589355618	21.44564682536868
52-53	21.30865417220399	30.248284843539352	27.063559746829085	21.37950123742757
54-55	21.308365316823448	30.42940111801323	27.02364343804596	21.23859012711736
56-57	21.26798326724262	30.246870275127502	26.914867189872023	21.570279267757854
58-59	21.314275056208896	29.986786668432792	27.16270301863189	21.536235256726425
60-61	21.422021250289923	30.333648337029672	26.899596884722065	21.344733527958333
62-63	21.191462609383997	30.198407792558772	27.14248886154389	21.467640736513342
64-65	21.341123541061133	30.081818648809655	27.054704984409106	21.522352825720105
66-67	21.14131124401538	30.098586922061628	27.030496890814515	21.72960494310848
68-69	21.41038849271115	30.262218444131587	26.889959489881093	21.437433573276163
70-71	21.346111753614128	30.053328815155012	27.07192528445932	21.52863414677154
72-73	21.281011257899017	30.164016619962393	27.0837386137369	21.471233508401692
74-75	21.235927813167553	30.173356116531654	27.127735732788732	21.462980337512064
76-77	21.278112057393653	30.04727025355265	26.967046148732717	21.707571540320984
78-79	21.343936701800388	30.010407861673194	27.16068550048781	21.48496993603861
80-81	21.154853620512277	30.08834697479325	27.152898021916428	21.603901382778044
82-83	21.1943635664135	30.063739492165837	27.28656394661532	21.45533299480534
84-85	21.13114253493199	29.977589009931112	27.139438684347788	21.75182977078911
86-87	21.37902056830332	29.92492421689514	27.101176914397968	21.594878300403575
88-89	21.209140717221043	29.91602428026973	27.265023062269083	21.609811940240142
90-91	21.227525345416296	29.897767241774254	27.080649307308036	21.794058105501417
92-93	21.29809303078	29.961144534205918	27.049629654049884	21.691132780964196
94-95	21.16110748216706	29.830686759772078	27.2442016118645	21.764004146196356
96-97	21.255758922316527	30.024903499657583	26.98034381128004	21.738993766745846
98-99	21.011347133811682	30.0845878641871	27.038309132146303	21.865755869854915
100	21.000099301401224	30.13809029219533	27.02930464864749	21.832505757755957
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	25053.0
1	18657.5
2	10139.0
3	6539.0
4	4246.0
5	3008.5
6	2413.5
7	2294.0
8	2447.5
9	2488.0
10	2086.5
11	1618.0
12	1495.0
13	1497.0
14	1495.5
15	1556.0
16	1703.5
17	2020.5
18	2463.5
19	3052.0
20	4043.5
21	5446.5
22	7478.0
23	10536.5
24	14017.0
25	17382.5
26	21796.5
27	27364.5
28	34678.0
29	42697.0
30	47817.0
31	52895.0
32	58273.0
33	62621.0
34	67590.5
35	73019.0
36	76502.5
37	78690.5
38	80624.5
39	81514.0
40	83509.5
41	84709.0
42	84808.0
43	86157.0
44	86909.0
45	85476.5
46	85512.0
47	85163.0
48	82661.0
49	81369.0
50	81396.0
51	78652.5
52	72349.0
53	66490.5
54	62974.0
55	61267.0
56	56264.5
57	49072.0
58	44189.0
59	40802.5
60	36371.0
61	31014.5
62	26516.0
63	21658.0
64	17015.0
65	13919.0
66	11401.5
67	9085.5
68	7166.5
69	5724.0
70	4591.5
71	3617.5
72	2923.5
73	2353.0
74	1971.0
75	1616.0
76	1178.5
77	895.0
78	710.0
79	533.0
80	359.0
81	233.5
82	174.0
83	135.5
84	105.5
85	92.0
86	76.0
87	67.5
88	61.0
89	44.0
90	42.0
91	49.0
92	38.5
93	29.5
94	24.5
95	19.5
96	17.0
97	24.0
98	24.0
99	17.0
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1731918479926448
2	0.16813515170088875
3	0.18031719276739197
4	0.17131474103585656
5	0.1862166717744407
6	0.07293901317805701
7	0.0743947287771989
8	0.06255746245786085
9	0.05650475022984983
10-11	0.06991265706405149
12-13	0.06864848299111248
14-15	0.06610098069261416
16-17	0.06263407906834202
18-19	0.0676333129022372
20-21	0.0685910205332516
22-23	0.06723107569721117
24-25	0.06654152620288079
26-27	0.0700658902850138
28-29	0.06822709163346613
30-31	0.07318801716212074
32-33	0.0703723567269384
34-35	0.06970196138522831
36-37	0.07996858718970273
38-39	0.0756589028501379
40-41	0.07123429359485137
42-43	0.07395418326693227
44-45	0.08379941771376034
46-47	0.07470119521912351
48-49	0.0796812749003984
50-51	0.07701884768617837
52-53	0.07525666564511185
54-55	0.07749770150168557
56-57	0.07140668096843396
58-59	0.07851287159056083
60-61	0.07527581979773215
62-63	0.06732684646031259
64-65	0.06995096536929206
66-67	0.07044897333741956
68-69	0.06866763714373277
70-71	0.06431964449892737
72-73	0.06165721728470733
74-75	0.06713530493410971
76-77	0.07234523444682807
78-79	0.0687634079068342
80-81	0.07629098988660742
82-83	0.07847456328532025
84-85	0.07973873735825927
86-87	0.07542905301869444
88-89	0.07765093472264786
90-91	0.07621437327612626
92-93	0.06734600061293289
94-95	0.06680968433956481
96-97	0.06629252221881704
98-99	0.07615691081826541
100	0.08347379711921545
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2610400.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.496963760046526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.86734167726691	44.1963875874893
2	13.556163504563115	15.588764834942614
3	4.205983336612518	7.254938144417086
4	1.830316389114248	4.209505403772845
5	0.9423722233557459	2.70917707873799
6	0.5699075802972118	1.9660773294554754
7	0.3716704859815501	1.4958947124212048
8	0.25569727990349167	1.1761453788922824
9	0.19541059983971798	1.0111964559581896
>10	1.0791643557161394	11.499570389526436
>50	0.08621779225723149	3.434556016374759
>100	0.03848923235360065	3.6157249627052654
>500	7.99290150585663E-4	0.3226164328897893
>1k	3.9964507529014707E-4	0.39587916272139856
>5k	0.0	0.0
>10k+	6.660751254835785E-5	1.1235661096953071
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29338	1.1238890591480233	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	1.9154152620288078E-5	0.0
14-15	0.0	0.0	0.0	3.8308305240576155E-5	0.0
16-17	0.0	0.0	0.0	3.8308305240576155E-5	3.8308305240576155E-5
18-19	0.0	0.0	0.0	5.746245786086423E-5	7.661661048115231E-5
20-21	0.0	0.0	0.0	1.5323322096230462E-4	1.3407906834201654E-4
22-23	0.0	0.0	0.0	5.937787312289305E-4	1.5323322096230462E-4
24-25	0.0	0.0	0.0	0.0011109408519767087	1.5323322096230462E-4
26-27	0.0	0.0	0.0	0.0017813361936867913	1.5323322096230462E-4
28-29	0.0	0.0	0.0	0.00339028501379099	1.5323322096230462E-4
30-31	0.0	0.0	0.0	0.0070295740116457246	1.5323322096230462E-4
32-33	0.0	0.0	0.0	0.01315890285013791	1.5323322096230462E-4
34-35	0.0	0.0	0.0	0.023368066196751455	1.5323322096230462E-4
36-37	0.0	0.0	0.0	0.03972571253447747	1.5323322096230462E-4
38-39	0.0	0.0	0.0	0.06318954949433037	2.2984983144345693E-4
40-41	0.0	0.0	0.0	0.08862626417407293	2.2984983144345693E-4
42-43	0.0	0.0	0.0	0.1142353662273981	2.2984983144345693E-4
44-45	0.0	3.8308305240576155E-5	0.0	0.14562902237205028	2.8731228930432113E-4
46-47	0.0	3.8308305240576155E-5	0.0	0.18546965982224944	3.0646644192460924E-4
48-49	0.0	3.8308305240576155E-5	0.0	0.2291028194912657	3.0646644192460924E-4
50-51	0.0	3.8308305240576155E-5	0.0	0.2768541219736439	3.0646644192460924E-4
52-53	0.0	3.8308305240576155E-5	0.0	0.3233029420778425	3.0646644192460924E-4
54-55	0.0	3.8308305240576155E-5	0.0	0.37457860864235365	3.0646644192460924E-4
56-57	0.0	3.8308305240576155E-5	0.0	0.43771069567882315	3.6392889978547347E-4
58-59	0.0	3.8308305240576155E-5	0.0	0.5101708550413729	4.9800796812749E-4
60-61	0.0	3.8308305240576155E-5	0.0	0.5885496475635918	4.9800796812749E-4
62-63	0.0	3.8308305240576155E-5	0.0	0.670606037388906	5.554704259883543E-4
64-65	0.0	3.8308305240576155E-5	0.0	0.7562825620594544	6.320870364695066E-4
66-67	0.0	3.8308305240576155E-5	0.0	0.8566120134845234	6.512411890897947E-4
68-69	0.0	3.8308305240576155E-5	0.0	0.9727627949739504	7.661661048115232E-4
70-71	0.0	3.8308305240576155E-5	0.0	1.1044667483910513	8.044744100520994E-4
72-73	0.0	3.8308305240576155E-5	0.0	1.2481420471958322	8.427827152926755E-4
74-75	0.0	3.8308305240576155E-5	0.0	1.3932922157523753	8.427827152926755E-4
76-77	0.0	3.8308305240576155E-5	0.0	1.5479619981612014	8.427827152926755E-4
78-79	0.0	3.8308305240576155E-5	0.0	1.7280493410971498	8.427827152926755E-4
80-81	0.0	3.8308305240576155E-5	0.0	1.9310450505669627	8.810910205332516E-4
82-83	0.0	3.8308305240576155E-5	0.0	2.1512028807845542	9.385534783941158E-4
84-85	0.0	3.8308305240576155E-5	0.0	2.371418173460006	9.76861783634692E-4
86-87	0.0	3.8308305240576155E-5	0.0	2.603183420165492	9.9601593625498E-4
88	0.0	3.8308305240576155E-5	0.0	2.7927903769537235	9.9601593625498E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4310	0.0	46.939323	1
GTATCAA	7725	0.0	38.61325	1
TCAACGC	9165	0.0	30.952599	4
ATCAACG	9170	0.0	30.886196	3
TATCAAC	9755	0.0	29.95033	2
CAACGCA	9970	0.0	28.40623	5
AACGCAG	10405	0.0	27.32493	6
GTACATG	17360	0.0	26.749338	1
TACATGG	17300	0.0	25.672205	2
ACGCAGA	11560	0.0	24.310234	7
CATGGGT	6880	0.0	24.206226	4
CGCAGAG	11535	0.0	24.036995	8
ACATGGG	17995	0.0	23.504179	3
TACCTGG	1750	0.0	22.582838	2
GCAGAGT	12650	0.0	21.138174	9
GTGGTAT	2220	0.0	20.76915	1
ATGGGTG	3720	0.0	20.234354	5
GTACCTG	2050	0.0	20.196423	1
TGGTATC	2270	0.0	19.689514	2
ATGGGTA	2855	0.0	19.279335	5
>>END_MODULE