FastQCFastQC Report
Wed 25 May 2016
SRR2431399_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431399_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2610400
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272191.0427137603432424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215920.8271529267545205No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149310.5719813055470426No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92830.3556159975482685No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50120.19200122586576768No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44490.17043365001532332No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44010.16859485136377567No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29930.11465675758504444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88650.064.762061
TATAACG2600.045.208192
GTATCAA178000.044.9596861
ATCAACG235400.033.914213
TCAACGC236850.033.7661364
TATCAAC237750.033.618542
GTGGTAT27350.033.3524781
CAACGCA244750.032.695455
TGGTATC28200.032.0112462
AACGCAG253650.031.5546826
ACGCAGA282000.028.29917
CGCAGAG284300.028.0205658
GCAGAGT299700.026.455289
GTACATG166950.026.2208631
ATAACGC4550.025.833253
TACATGG167650.025.800892
TAACGCA4750.025.7353574
CATGGGT69000.025.4161764
ACATGGG169250.024.7513833
GAGTACT204100.023.38411712-13
Produced by FastQC (version 0.11.5)