##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431399_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2610400
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.59653616304015	34.0	31.0	34.0	31.0	34.0
2	32.68094544897334	34.0	31.0	34.0	31.0	34.0
3	32.775894115844316	34.0	31.0	34.0	31.0	34.0
4	36.272857416487895	37.0	35.0	37.0	35.0	37.0
5	36.18780570027582	37.0	37.0	37.0	35.0	37.0
6	36.24175758504444	37.0	37.0	37.0	35.0	37.0
7	36.19241495556236	37.0	37.0	37.0	35.0	37.0
8	36.2056520073552	37.0	37.0	37.0	35.0	37.0
9	37.983728930432115	39.0	39.0	39.0	35.0	39.0
10-11	37.95749310450506	39.0	38.0	39.0	35.0	39.0
12-13	37.94890840484217	39.0	38.0	39.0	35.0	39.0
14-15	39.370712726019	41.0	39.0	41.0	36.0	41.0
16-17	39.40248161201349	41.0	39.0	41.0	36.0	41.0
18-19	39.48621667177444	41.0	39.5	41.0	36.0	41.0
20-21	39.47633734293595	41.0	39.0	41.0	36.0	41.0
22-23	39.40641185258964	41.0	39.0	41.0	36.0	41.0
24-25	39.35152965062826	41.0	39.0	41.0	36.0	41.0
26-27	39.22266070334048	41.0	39.0	41.0	35.0	41.0
28-29	39.13112377413424	41.0	39.0	41.0	35.0	41.0
30-31	38.91369043058535	41.0	38.5	41.0	35.0	41.0
32-33	38.648391817346	41.0	38.0	41.0	35.0	41.0
34-35	38.49080830524058	40.5	38.0	41.0	35.0	41.0
36-37	38.33929665951578	40.0	38.0	41.0	35.0	41.0
38-39	38.192734255286545	40.0	38.0	41.0	34.0	41.0
40-41	38.04414419246093	40.0	38.0	41.0	34.0	41.0
42-43	37.84905761569108	40.0	37.0	41.0	33.5	41.0
44-45	37.5700438630095	40.0	37.0	41.0	33.0	41.0
46-47	37.14474754826847	40.0	37.0	41.0	32.5	41.0
48-49	36.7717273214833	40.0	37.0	41.0	31.0	41.0
50-51	36.595432309224634	40.0	36.0	41.0	31.0	41.0
52-53	36.530727666258045	40.0	36.0	41.0	31.0	41.0
54-55	36.37935124885075	40.0	35.5	41.0	31.0	41.0
56-57	36.212573360404534	40.0	35.0	41.0	30.0	41.0
58-59	36.01538997854735	39.0	35.0	41.0	30.0	41.0
60-61	35.8416556849525	39.0	35.0	41.0	30.0	41.0
62-63	35.66253409439166	39.0	35.0	41.0	29.5	41.0
64-65	35.41275532485443	38.5	35.0	41.0	29.0	41.0
66-67	35.13119598528961	37.5	35.0	41.0	29.0	41.0
68-69	34.82657696138523	37.0	35.0	40.0	29.0	41.0
70-71	34.52369751762182	36.5	35.0	39.5	28.0	41.0
72-73	34.200224486668716	36.0	35.0	39.0	28.0	41.0
74-75	33.86885055930126	35.5	34.5	39.0	27.5	41.0
76-77	33.55203493717438	35.0	34.0	38.0	27.0	40.5
78-79	33.24791947594238	35.0	34.0	37.0	27.0	39.0
80-81	33.00704355654306	35.0	34.0	37.0	27.0	39.0
82-83	32.833890016855655	35.0	34.0	36.0	27.0	39.0
84-85	32.59542962764327	35.0	34.0	36.0	27.0	37.0
86-87	32.37706998927368	35.0	34.0	36.0	27.0	37.0
88-89	32.16347379711921	35.0	34.0	35.5	26.0	37.0
90-91	31.997070563898255	35.0	34.0	35.0	26.0	36.0
92-93	31.84785052099295	35.0	34.0	35.0	26.0	36.0
94-95	31.711763331290225	35.0	34.0	35.0	25.0	36.0
96-97	31.575097494636836	35.0	34.0	35.0	25.0	36.0
98-99	31.4640767698437	35.0	34.0	35.0	25.0	36.0
100	31.36140093472265	35.0	34.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.004070189860932771
1101	2	0.04310722640629194
1101	3	-0.026114218228784125
1101	4	0.10423185239949362
1101	5	0.06947132788120314
1101	6	0.05985189176224992
1101	7	0.08806607310242498
1101	8	0.10955168490563949
1101	9	0.07933649947405996
1101	10-11	0.11652198819770376
1101	12-13	0.07090564085061146
1101	14-15	0.16315665147686076
1101	16-17	0.0805719434384855
1101	18-19	0.03637252607674668
1101	20-21	0.13246673916894025
1101	22-23	0.08771290800984843
1101	24-25	0.10289936856335657
1101	26-27	0.09002687234896456
1101	28-29	0.017111878654290535
1101	30-31	0.07418835959921921
1101	32-33	0.13264199799620968
1101	34-35	0.15546088016466797
1101	36-37	0.12061087632079648
1101	38-39	0.06342137873767228
1101	40-41	0.11586377198486275
1101	42-43	0.11384598239923349
1101	44-45	0.1731234309899321
1101	46-47	0.14535411618981442
1101	48-49	0.2634429959449349
1101	50-51	0.20059449447516187
1101	52-53	0.25171691003153285
1101	54-55	0.19913486012927706
1101	56-57	0.15652961920188346
1101	58-59	0.18445372556934103
1101	60-61	0.11097774596652954
1101	62-63	0.17411755670761409
1101	64-65	0.1779908768941425
1101	66-67	0.1277744238304237
1101	68-69	0.1034816609606537
1101	70-71	0.17542566718382346
1101	72-73	0.15909078098042784
1101	74-75	0.15982779461955232
1101	76-77	0.09137215288326672
1101	78-79	0.14841709788813517
1101	80-81	0.18591831696915762
1101	82-83	0.14912933135592255
1101	84-85	0.1388443789396021
1101	86-87	0.19852444850218376
1101	88-89	0.23728159955355466
1101	90-91	0.22828145534594668
1101	92-93	0.22374573569313227
1101	94-95	0.19961197823231203
1101	96-97	0.22621858031786957
1101	98-99	0.2166733945322612
1101	100	0.2582200858271655
1102	1	-0.02551480408321538
1102	2	-0.016062414660382274
1102	3	-0.036931792623583704
1102	4	0.04919542329707838
1102	5	0.00907206544593464
1102	6	-0.009733218713961378
1102	7	-0.03671888882315244
1102	8	-0.02878498496728099
1102	9	-3.9425319197761155E-4
1102	10-11	0.0367456284887453
1102	12-13	-0.02827337688536602
1102	14-15	-0.07854662972618343
1102	16-17	-0.05558068665204985
1102	18-19	-0.007019297797796753
1102	20-21	0.031208074957959298
1102	22-23	0.0416300258278568
1102	24-25	0.08323368102036
1102	26-27	0.05194405509197253
1102	28-29	0.03816079486101387
1102	30-31	-0.0124514959065678
1102	32-33	0.009859167913759848
1102	34-35	-0.06005351907126766
1102	36-37	-0.07027578910241061
1102	38-39	-0.017658093840992706
1102	40-41	0.03565627832112028
1102	42-43	0.0032758166746873485
1102	44-45	-0.09489670935767691
1102	46-47	-0.22802422395381683
1102	48-49	-0.3333513110198041
1102	50-51	-0.36913246604328975
1102	52-53	-0.30590739794661204
1102	54-55	-0.3556906667465256
1102	56-57	-0.2576204115299774
1102	58-59	-0.3175609265342416
1102	60-61	-0.390452719801722
1102	62-63	-0.3671733952088232
1102	64-65	-0.38831167100313735
1102	66-67	-0.4144069232289169
1102	68-69	-0.3427762829018235
1102	70-71	-0.36676670880299866
1102	72-73	-0.3241621047661951
1102	74-75	-0.2782104768686722
1102	76-77	-0.3434735562361837
1102	78-79	-0.2948757985877606
1102	80-81	-0.30103460053938846
1102	82-83	-0.2751814283062046
1102	84-85	-0.3060438623023103
1102	86-87	-0.28125078750473165
1102	88-89	-0.3444474005534701
1102	90-91	-0.3630287694478689
1102	92-93	-0.32913460744940437
1102	94-95	-0.38062731897390734
1102	96-97	-0.401269058439393
1102	98-99	-0.37710880135217195
1102	100	-0.2857427104790169
1103	1	-0.0802125271923515
1103	2	-0.07547094645833852
1103	3	-0.05368133462829405
1103	4	-0.05601263742001095
1103	5	-0.019192786687014518
1103	6	-0.049513380975149346
1103	7	-0.05242158445256706
1103	8	-0.012558866150214953
1103	9	-0.07681403858846636
1103	10-11	-0.009053900430387785
1103	12-13	0.024461509270096826
1103	14-15	-0.01966152111586439
1103	16-17	0.042430305234887555
1103	18-19	0.03982707749662495
1103	20-21	-0.05829729012971541
1103	22-23	-0.028770392910693943
1103	24-25	-0.020142398539960027
1103	26-27	-0.03664531941731752
1103	28-29	-0.0704495165757848
1103	30-31	-0.05851273641953014
1103	32-33	-0.02900500376904347
1103	34-35	-0.0421262748943505
1103	36-37	-0.10482171948711994
1103	38-39	-0.13909227337514096
1103	40-41	-0.16193597501570167
1103	42-43	-0.11109214982622717
1103	44-45	-0.10431832673533137
1103	46-47	-0.15225871754189058
1103	48-49	-0.08119219037074998
1103	50-51	-0.1990199300579505
1103	52-53	-0.16523741626642874
1103	54-55	-0.08952997582791511
1103	56-57	-0.11407160231773616
1103	58-59	-0.09929345728535566
1103	60-61	-0.06632291085119135
1103	62-63	-0.05534403116799069
1103	64-65	-0.016288640382882136
1103	66-67	-0.038196507107478794
1103	68-69	-0.07151221590365964
1103	70-71	-0.057161893309675804
1103	72-73	-0.12264417752202661
1103	74-75	-0.11830469304243962
1103	76-77	-0.02850365306947822
1103	78-79	-0.05357770908239701
1103	80-81	-0.013544414724158571
1103	82-83	-0.0956472749432038
1103	84-85	-0.13710902848917073
1103	86-87	-0.11427879064527247
1103	88-89	-0.1490796957641507
1103	90-91	-0.10916852343897077
1103	92-93	-0.1519558584308207
1103	94-95	-0.11917743674412407
1103	96-97	-0.046649848808403505
1103	98-99	-0.040547691695014265
1103	100	0.0029251775084198073
1104	1	-0.10548757892235727
1104	2	-0.0945532579193582
1104	3	-0.07329236680396178
1104	4	0.00543716225091373
1104	5	-0.06387708297316408
1104	6	-0.07464154672913281
1104	7	-0.036254288824970615
1104	8	-0.13389335585801376
1104	9	-0.08900158425384319
1104	10-11	-0.07887589113920512
1104	12-13	-0.04054148624062748
1104	14-15	-0.12425733906917458
1104	16-17	-0.09275391523470233
1104	18-19	-0.08157242731532222
1104	20-21	-0.07424105763735156
1104	22-23	-0.15007715660053123
1104	24-25	-0.05451399212689978
1104	26-27	-0.07993099977527152
1104	28-29	-0.04642997874580601
1104	30-31	-0.028606820499128105
1104	32-33	-0.024597908495294973
1104	34-35	1.1046087074362276E-5
1104	36-37	-0.07285716393312214
1104	38-39	-0.04153910477975842
1104	40-41	-0.06748849882636421
1104	42-43	-0.008222052893863463
1104	44-45	0.008607714834163005
1104	46-47	-0.11664825942551005
1104	48-49	-0.2538271059997257
1104	50-51	-0.2580851201868768
1104	52-53	-0.16293519358922026
1104	54-55	-0.0553540467448812
1104	56-57	-0.15229817878608287
1104	58-59	-0.07880609718478837
1104	60-61	-0.11484781101687958
1104	62-63	-0.11974775253624159
1104	64-65	-0.1030241900364004
1104	66-67	-0.1332305640142053
1104	68-69	-0.1404759040392065
1104	70-71	-0.12595516619340685
1104	72-73	-0.15176230989745676
1104	74-75	-0.16022495301479722
1104	76-77	-0.14146887569908273
1104	78-79	-0.12804346380386988
1104	80-81	-0.07904159382542986
1104	82-83	-0.13457714972067691
1104	84-85	-0.1542350389064424
1104	86-87	-0.11887914245610176
1104	88-89	-0.07474675761257288
1104	90-91	-0.12569783967821735
1104	92-93	-0.20916605412009304
1104	94-95	-0.1865545843409322
1104	96-97	-0.2134367074189214
1104	98-99	-0.24210954713389654
1104	100	-0.0892263367174344
1105	1	-0.15991065171002106
1105	2	-0.1320904171911863
1105	3	-0.17758358389141193
1105	4	-0.09103131528122077
1105	5	-0.07729879011250773
1105	6	0.013538449911870032
1105	7	0.02079160724802165
1105	8	-0.006477509818871852
1105	9	-0.002943078867055249
1105	10-11	-0.07312602036813587
1105	12-13	-0.10285650649397837
1105	14-15	-0.08958485648819448
1105	16-17	-0.10982696911779044
1105	18-19	-0.16136894380966282
1105	20-21	-0.13056200595313072
1105	22-23	-0.15035466875240644
1105	24-25	-0.13383823438534392
1105	26-27	-0.054831937872499736
1105	28-29	-0.11766295203467791
1105	30-31	-0.07326062170787395
1105	32-33	-0.10617168004467459
1105	34-35	-0.1166141842997348
1105	36-37	-0.09436677151542483
1105	38-39	-0.04724617144501053
1105	40-41	-0.18232068631624543
1105	42-43	-0.2404391926105518
1105	44-45	-0.1672070875197491
1105	46-47	-0.17190836003761945
1105	48-49	-0.08187688833165652
1105	50-51	-0.1472051671257688
1105	52-53	-0.19383179601841505
1105	54-55	-0.10994568196515786
1105	56-57	-0.09256464080965543
1105	58-59	-0.13013240520068337
1105	60-61	-0.11897588933982917
1105	62-63	-0.2574476422663281
1105	64-65	-0.2821964947319415
1105	66-67	-0.29814532463921495
1105	68-69	-0.1526105618091549
1105	70-71	-0.17308119373012687
1105	72-73	-0.1556626213806851
1105	74-75	-0.1844371397043929
1105	76-77	-0.22490346898386093
1105	78-79	-0.1789946404472218
1105	80-81	-0.15802442400296002
1105	82-83	-0.2467375852255742
1105	84-85	-0.19597537082676553
1105	86-87	-0.2594038990171015
1105	88-89	-0.14368195342128587
1105	90-91	-0.20193617130619046
1105	92-93	-0.23474950334435363
1105	94-95	-0.2159140157935262
1105	96-97	-0.24412357653471872
1105	98-99	-0.15896168691726942
1105	100	-0.17723253099553915
1106	1	-0.08720785721335034
1106	2	-0.07304231641478509
1106	3	-0.0580076079957621
1106	4	0.02642758557701086
1106	5	-0.013007490862555926
1106	6	-0.034133641468550024
1106	7	-0.011012451588086947
1106	8	0.0017561556267295941
1106	9	0.039586844671426036
1106	10-11	-0.006456429595161239
1106	12-13	-0.017118840539907865
1106	14-15	-0.00406739229084252
1106	16-17	-0.005938448408826957
1106	18-19	0.010506041707728286
1106	20-21	0.03173367349773315
1106	22-23	-0.047002186384766276
1106	24-25	-0.027586219234720488
1106	26-27	0.008880884874351125
1106	28-29	-0.02202855448112473
1106	30-31	-0.07815057710477191
1106	32-33	-0.06413436359303404
1106	34-35	-0.02787688181759762
1106	36-37	-0.046491526469800704
1106	38-39	-0.028150653802057946
1106	40-41	-0.006945148308268756
1106	42-43	-0.006515476695973632
1106	44-45	-0.0398048459108864
1106	46-47	-0.06384491068236997
1106	48-49	-0.040849381649799454
1106	50-51	-0.13436106877780674
1106	52-53	-0.13883349420694913
1106	54-55	-0.05914733473625233
1106	56-57	-0.03819019983809824
1106	58-59	-0.039268162960212294
1106	60-61	-0.10726775663880517
1106	62-63	-0.14380850699849645
1106	64-65	-0.08171904886046377
1106	66-67	-0.10668830536063467
1106	68-69	-0.09856847586426198
1106	70-71	-0.09715925611356369
1106	72-73	-0.036445615396310416
1106	74-75	-0.03751854086551987
1106	76-77	-0.051513222610800824
1106	78-79	0.030969124910122048
1106	80-81	-2.9839444719925723E-4
1106	82-83	-0.03924675312643444
1106	84-85	-0.048774237112134244
1106	86-87	-0.05879546421728321
1106	88-89	-0.08306287928822798
1106	90-91	-0.12161625642085738
1106	92-93	-0.09393938465653306
1106	94-95	9.284393170290173E-4
1106	96-97	0.021549213982968496
1106	98-99	-0.007555732465799991
1106	100	0.05131464719773504
1107	1	-0.23532609572470875
1107	2	-0.19079856647572058
1107	3	-0.14164842082053042
1107	4	-0.20879048817256773
1107	5	-0.26830537820709566
1107	6	-0.3229272407822066
1107	7	-0.2498211621890576
1107	8	-0.12363698157400904
1107	9	-0.19850919812113688
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2216	24-25	-0.20781064334033772
2216	26-27	-0.16354246176202736
2216	28-29	-0.1934512833271782
2216	30-31	-0.20732836191236714
2216	32-33	-0.2244104747666995
2216	34-35	-0.17753212263965423
2216	36-37	-0.1611100126392202
2216	38-39	-0.15279506691017275
2216	40-41	-0.13705703567859473
2216	42-43	-0.07356217080278071
2216	44-45	-0.047150742371137255
2216	46-47	-0.05522867167317003
2216	48-49	-0.04206667286853616
2216	50-51	0.028153445124857512
2216	52-53	-0.034382991008683916
2216	54-55	0.06677823374107561
2216	56-57	0.11117002673665866
2216	58-59	0.1072012576096455
2216	60-61	0.007523377206034354
2216	62-63	-0.0018560154923648042
2216	64-65	-0.023644821083486534
2216	66-67	-0.09512216217745362
2216	68-69	-0.11240677088208884
2216	70-71	-0.025215277841674322
2216	72-73	-0.04187990651317364
2216	74-75	-0.08162632830036642
2216	76-77	-0.119298494894295
2216	78-79	-0.04058982164118419
2216	80-81	-0.05344659323390033
2216	82-83	-0.012041637798617444
2216	84-85	-0.019831296036933566
2216	86-87	-0.006766198708632487
2216	88-89	5.154254879329301E-4
2216	90-91	-0.08456364058219279
2216	92-93	-0.06195681980791079
2216	94-95	-0.05107258775196044
2216	96-97	-0.04212396956748776
2216	98-99	-0.09774212999749565
2216	100	-0.12384839404647252
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	34.0
3	246.0
4	76.0
5	96.0
6	317.0
7	628.0
8	1083.0
9	1764.0
10	2653.0
11	3870.0
12	5987.0
13	8528.0
14	9050.0
15	11558.0
16	15305.0
17	24404.0
18	28033.0
19	13766.0
20	8734.0
21	7837.0
22	7934.0
23	7686.0
24	8389.0
25	9770.0
26	11932.0
27	15224.0
28	19412.0
29	24273.0
30	30279.0
31	38486.0
32	48458.0
33	62940.0
34	89516.0
35	154172.0
36	239853.0
37	483318.0
38	780788.0
39	430288.0
40	3713.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.68145560617246	20.591945795001916	14.797189069659906	21.92940952916572
2	14.942678453419658	21.856471061478196	40.427502849316646	22.773347635785502
3	16.582508963264694	29.1843285471792	31.649183240581724	22.58397924897438
4	11.214641434262948	18.618717437940546	38.94862856267239	31.21801256512412
5	11.376551764312717	39.11956058510981	35.05925754458688	14.4446301059906
6	27.029880478087648	39.67047195832056	18.69889672080907	14.600750842782714
7	24.341480232914495	35.27440239043825	23.412503830830524	16.971613545816734
8	25.6398253141281	34.26739197057922	20.84841403616304	19.244368679129636
9	26.732475277716272	15.509627267566096	21.860804367763077	35.897093086954555
10-11	23.363794820717132	27.343319031566043	28.990844315047504	20.302041832669325
12-13	25.220157830217595	25.949605424456024	29.149249157217284	19.680987588109105
14-15	22.438323628562674	26.426850291143122	28.52857799570947	22.606248084584738
16-17	19.596153846153847	29.626110940851973	29.15761952191235	21.620115691081825
18-19	19.58327435447117	26.8585237081618	33.689377311548405	19.868824625818625
20-21	21.286028961078763	25.80460848912044	33.641702421084894	19.267660128715907
22-23	20.82367484817455	25.868420881252252	33.773208616767015	19.534695653806182
24-25	20.32116156933067	26.108474967145835	33.77483025107843	19.79553321244507
26-27	20.0043709826513	26.145065290835074	33.827287573499355	20.023276153014276
28-29	20.001114771896024	26.051491352185746	34.038927757992596	19.908466117925627
30-31	20.25215714397356	26.295320709961246	33.644148244174445	19.808373901890747
32-33	19.988066825775654	26.278477763688674	33.80760198745772	19.925853423077953
34-35	20.373171507689587	26.043626190271425	33.540001720055095	20.043200581983896
36-37	20.41981340604833	26.209730866151748	33.04401974582952	20.326435981970402
38-39	20.319558960595376	26.35525339423892	33.1209950738954	20.2041925712703
40-41	20.31129747697381	26.14677001237743	33.14030394610475	20.401628564544012
42-43	20.293112952723977	26.475017363285914	32.831703885200426	20.400165798789686
44-45	20.60190856063437	26.403632551606627	32.487344355541396	20.507114532217617
46-47	20.73409084205135	26.63209723877461	31.851950602187635	20.781861316986408
48-49	20.86560330432244	26.84275468999184	31.251122912627533	21.040519093058187
50-51	20.932200094660022	26.99162904304311	30.946452729654467	21.129718132642402
52-53	20.920303977857692	27.159933151687248	30.849921707026258	21.0698411634288
54-55	20.916455730146826	27.3206704381446	30.853179562765227	20.909694268943344
56-57	20.884029078159813	27.102960376103685	30.751751493289774	21.261259052446725
58-59	20.838511363061738	26.725145840388482	31.079479372415136	21.35686342413465
60-61	20.97481452366874	27.06275557884724	30.90111434244946	21.061315555034557
62-63	20.836109113741042	26.86525739385537	31.188387730967598	21.11024576143599
64-65	20.857040080172602	26.837901782430677	31.141911791747372	21.16314634564935
66-67	20.859119680446582	26.882829520305947	31.003741582206207	21.254309217041264
68-69	21.15960566902493	27.03095622273402	30.742806838502663	21.066631269738384
70-71	21.100270054863334	26.91076888540453	30.776083547493087	21.212877512239046
72-73	21.03013449678344	26.98447783996074	30.72128492332775	21.264102739928063
74-75	21.00724412827202	27.09731112975456	30.591674041043674	21.303770700929746
76-77	21.0704319342871	27.052058019782905	30.46061587455186	21.416894171378136
78-79	21.044490006068074	27.096454008299165	30.577685122188917	21.281370863443847
80-81	20.84939659226186	27.09212155689827	30.575001939370956	21.483479911468915
82-83	20.80768719494232	27.08124949480284	30.72502831977266	21.38603499048218
84-85	20.846571366237782	27.11875087750752	30.407356946402174	21.627320809852524
86-87	20.9425377448372	27.01645790211837	30.418042436341082	21.62296191670335
88-89	20.79226240699364	27.01861515933071	30.52640954499789	21.662712888677767
90-91	20.731538431922917	27.044633999974334	30.373637815708886	21.850189752393863
92-93	20.780500885888042	27.084326964516592	30.326811281903943	21.808360867691423
94-95	20.63629791972896	26.95547125113154	30.50299937058996	21.905231458549537
96-97	20.797143116545108	27.12005108801458	30.200482915050852	21.882322880389456
98-99	20.505337605230782	27.084288250028205	30.314375974350227	22.095998170390786
100	20.603915802845314	27.143955497956824	30.226781596822246	22.025347102375616
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2436.0
1	1437.5
2	3984.0
3	6488.0
4	3428.0
5	1708.0
6	3579.5
7	9474.0
8	16619.0
9	17904.0
10	11253.0
11	4950.0
12	3673.0
13	3663.0
14	3927.5
15	4189.0
16	4476.5
17	5010.5
18	5498.0
19	6026.5
20	6876.5
21	8120.5
22	9882.5
23	12594.5
24	15872.5
25	18895.0
26	22989.0
27	28093.0
28	34695.0
29	42334.5
30	47787.0
31	52592.0
32	57211.5
33	61682.5
34	66811.5
35	71971.5
36	75311.5
37	77241.0
38	79344.5
39	80834.0
40	82735.5
41	83985.5
42	84235.0
43	85557.0
44	86743.5
45	86056.5
46	85499.0
47	84968.0
48	82477.0
49	80261.5
50	80830.0
51	78615.0
52	71347.0
53	64754.5
54	61014.5
55	58820.0
56	53268.5
57	46230.5
58	42331.5
59	39362.0
60	34871.0
61	29393.0
62	25033.0
63	20368.5
64	15616.0
65	12551.5
66	10114.5
67	7774.5
68	5947.0
69	4617.5
70	3588.0
71	2746.5
72	2184.0
73	1782.5
74	1513.0
75	1183.5
76	757.0
77	573.5
78	466.0
79	284.5
80	135.5
81	70.0
82	34.0
83	18.5
84	10.0
85	11.5
86	7.5
87	3.0
88	3.0
89	1.0
90	0.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006167637143732762
2	0.00478853815507202
3	8.810910205332516E-4
4	0.0
5	0.04259883542752069
6	0.0
7	0.0
8	0.0
9	7.661661048115231E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	5.746245786086424E-5
20-21	0.0
22-23	3.8308305240576155E-5
24-25	1.9154152620288078E-5
26-27	1.1492491572172848E-4
28-29	1.9154152620288078E-5
30-31	1.5323322096230462E-4
32-33	0.0011492491572172847
34-35	7.087036469506589E-4
36-37	9.9601593625498E-4
38-39	8.044744100520993E-4
40-41	1.1492491572172848E-4
42-43	2.681581366840331E-4
44-45	1.3407906834201656E-4
46-47	3.8308305240576155E-5
48-49	3.8308305240576155E-5
50-51	2.1069567882316887E-4
52-53	4.788538155072019E-4
54-55	6.703953417100828E-4
56-57	0.002145265093472265
58-59	4.596996628869139E-4
60-61	0.0010151700888752682
62-63	8.810910205332516E-4
64-65	0.001225865767698437
66-67	2.681581366840331E-4
68-69	1.5323322096230462E-4
70-71	1.723873735825927E-4
72-73	1.1492491572172848E-4
74-75	3.830830524057616E-4
76-77	5.554704259883543E-4
78-79	6.129328838492185E-4
80-81	6.703953417100828E-4
82-83	0.0012641740729390132
84-85	9.002451731535397E-4
86-87	0.0016472571253447748
88-89	0.0010534783941158444
90-91	9.385534783941157E-4
92-93	9.577076310144038E-4
94-95	7.278577995709469E-4
96-97	1.1492491572172848E-4
98-99	2.49003984063745E-4
100	1.1492491572172848E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2610400.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.16114990922562
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.72838707922192	37.2086791401626
2	15.708700671177125	16.07350379834492
3	5.062945909013901	7.770784039000822
4	2.190344194730391	4.482421107976144
5	1.106992683782863	2.8317509321715524
6	0.6650234319172842	2.0414018096080726
7	0.4735327057356196	1.6958534421542881
8	0.3238352314669646	1.3254226258376123
9	0.2434536594827755	1.120983225187306
>10	1.3292576582313835	12.636385305400735
>50	0.11011025720730183	3.8899243083054023
>100	0.05464169723113214	4.597276955551679
>500	0.0019498740765506208	0.7041749623831616
>1k	4.499709407406228E-4	0.627284719662626
>5k	1.4999031358020758E-4	0.5484760577146761
>10k+	2.249854703703114E-4	2.445677570538572
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27219	1.0427137603432424	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21592	0.8271529267545205	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14931	0.5719813055470426	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9283	0.3556159975482685	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5012	0.19200122586576768	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4449	0.17043365001532332	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4401	0.16859485136377567	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2993	0.11465675758504444	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	3.8308305240576155E-5
3	0.0	0.0	0.0	0.0	3.8308305240576155E-5
4	0.0	0.0	0.0	0.0	3.8308305240576155E-5
5	0.0	0.0	0.0	0.0	3.8308305240576155E-5
6	0.0	0.0	0.0	0.0	3.8308305240576155E-5
7	0.0	0.0	0.0	0.0	3.8308305240576155E-5
8	0.0	0.0	0.0	0.0	7.661661048115231E-5
9	0.0	0.0	0.0	0.0	7.661661048115231E-5
10-11	0.0	0.0	0.0	1.9154152620288078E-5	7.661661048115231E-5
12-13	0.0	0.0	0.0	3.8308305240576155E-5	7.661661048115231E-5
14-15	0.0	0.0	0.0	3.8308305240576155E-5	7.661661048115231E-5
16-17	0.0	0.0	0.0	3.8308305240576155E-5	1.1492491572172847E-4
18-19	0.0	0.0	0.0	5.746245786086423E-5	1.1492491572172847E-4
20-21	0.0	0.0	0.0	1.5323322096230462E-4	1.1492491572172847E-4
22-23	0.0	0.0	0.0	5.937787312289305E-4	1.5323322096230462E-4
24-25	0.0	0.0	0.0	0.0011109408519767087	1.5323322096230462E-4
26-27	0.0	0.0	0.0	0.0017813361936867913	1.723873735825927E-4
28-29	0.0	0.0	0.0	0.0035435182347532948	1.915415262028808E-4
30-31	0.0	0.0	0.0	0.007297732148329758	1.915415262028808E-4
32-33	0.0	0.0	0.0	0.01367606497088569	1.915415262028808E-4
34-35	0.0	0.0	0.0	0.023961844927980386	1.915415262028808E-4
36-37	0.0	0.0	0.0	0.040530186944529575	1.915415262028808E-4
38-39	0.0	0.0	0.0	0.06439626110940852	1.915415262028808E-4
40-41	0.0	0.0	0.0	0.09017775053631627	1.915415262028808E-4
42-43	0.0	0.0	0.0	0.1159975482684646	1.915415262028808E-4
44-45	0.0	0.0	0.0	0.14809990806006743	1.915415262028808E-4
46-47	0.0	0.0	0.0	0.18878332822555932	1.915415262028808E-4
48-49	0.0	0.0	0.0	0.23302942077842476	1.915415262028808E-4
50-51	0.0	0.0	0.0	0.2818342016549188	1.915415262028808E-4
52-53	0.0	0.0	0.0	0.3288768004903463	2.2984983144345693E-4
54-55	0.0	0.0	0.0	0.3811484829911125	2.2984983144345693E-4
56-57	0.0	0.0	0.0	0.44550643579528043	2.49003984063745E-4
58-59	0.0	0.0	0.0	0.5192499233833896	2.6815813668403307E-4
60-61	0.0	0.0	0.0	0.5992185105730923	3.0646644192460924E-4
62-63	0.0	0.0	0.0	0.6827689243027888	3.0646644192460924E-4
64-65	0.0	0.0	0.0	0.7700160894882011	3.0646644192460924E-4
66-67	0.0	0.0	0.0	0.8727206558381857	3.0646644192460924E-4
68-69	0.0	0.0	0.0	0.9917637143732762	3.0646644192460924E-4
70-71	0.0	0.0	0.0	1.126723873735826	3.6392889978547347E-4
72-73	0.0	0.0	0.0	1.2742874655225251	4.2139135764633775E-4
74-75	0.0	0.0	0.0	1.4230003064664418	4.2139135764633775E-4
76-77	0.0	0.0	0.0	1.5811561446521605	4.2139135764633775E-4
78-79	0.0	0.0	0.0	1.7659937174379405	4.2139135764633775E-4
80-81	0.0	0.0	0.0	1.974601593625498	4.405455102666258E-4
82-83	0.0	0.0	0.0	2.2009462151394423	4.9800796812749E-4
84-85	0.0	0.0	0.0	2.4278271529267546	5.363162733680661E-4
86-87	0.0	0.0	0.0	2.6669092859331904	5.363162733680661E-4
88	0.0	0.0	0.0	2.8635458167330676	5.363162733680661E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8865	0.0	64.76206	1
TATAACG	260	0.0	45.20819	2
GTATCAA	17800	0.0	44.959686	1
ATCAACG	23540	0.0	33.91421	3
TCAACGC	23685	0.0	33.766136	4
TATCAAC	23775	0.0	33.61854	2
GTGGTAT	2735	0.0	33.352478	1
CAACGCA	24475	0.0	32.69545	5
TGGTATC	2820	0.0	32.011246	2
AACGCAG	25365	0.0	31.554682	6
ACGCAGA	28200	0.0	28.2991	7
CGCAGAG	28430	0.0	28.020565	8
GCAGAGT	29970	0.0	26.45528	9
GTACATG	16695	0.0	26.220863	1
ATAACGC	455	0.0	25.83325	3
TACATGG	16765	0.0	25.80089	2
TAACGCA	475	0.0	25.735357	4
CATGGGT	6900	0.0	25.416176	4
ACATGGG	16925	0.0	24.751383	3
GAGTACT	20410	0.0	23.384117	12-13
>>END_MODULE