FastQCFastQC Report
Wed 25 May 2016
SRR2431398_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431398_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3050834
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA297510.9751759682762157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56200.046.3024831
GTATCAA96200.037.224171
TCAACGC111500.030.7205624
ATCAACG112000.030.6699563
TATCAAC123350.028.6489752
CAACGCA120450.028.4378815
GTACATG201350.027.8339981
TACATGG201200.026.8720232
AACGCAG129950.026.5787646
CATGGGT90200.026.4990224
ACATGGG204000.025.3037433
CGCAGAG140250.024.0244968
ACGCAGA145250.023.5533777
ATGGGTG48700.023.1875555
TACCTGG21800.022.448392
GCAGAGT153200.021.2885369
GTACCTG26250.020.435771
ATGGGTA37300.020.3090535
CATGGGG83450.019.4520514
GTGGTAT28550.018.7894551
Produced by FastQC (version 0.11.5)