##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431398_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3050834 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.4654622309834 31.0 31.0 34.0 26.0 34.0 2 30.599483616611064 33.0 31.0 34.0 26.0 34.0 3 30.720637045476746 34.0 31.0 34.0 26.0 34.0 4 33.8559472590118 37.0 35.0 37.0 28.0 37.0 5 34.0347262420702 37.0 35.0 37.0 30.0 37.0 6 34.03594623634062 37.0 35.0 37.0 30.0 37.0 7 33.950120196641315 37.0 35.0 37.0 30.0 37.0 8 33.90447005638458 37.0 35.0 37.0 29.0 37.0 9 35.304863194785426 39.0 37.0 39.0 27.0 39.0 10-11 35.335571846911364 39.0 37.0 39.0 27.5 39.0 12-13 35.264938538117775 39.0 37.0 39.0 27.0 39.0 14-15 36.59464756194535 40.0 38.0 41.0 27.0 41.0 16-17 36.628414885896774 40.0 38.0 41.0 27.5 41.0 18-19 36.65324662043231 40.0 38.0 41.0 28.0 41.0 20-21 36.73605643571561 40.5 38.0 41.0 28.0 41.0 22-23 36.722837427405096 40.0 38.0 41.0 28.0 41.0 24-25 36.779984915600124 40.0 38.0 41.0 29.0 41.0 26-27 36.824513559243144 40.0 38.0 41.0 29.5 41.0 28-29 36.7901416465137 40.0 38.0 41.0 30.0 41.0 30-31 36.716768103410416 40.0 37.5 41.0 30.0 41.0 32-33 36.65642067054451 40.0 37.0 41.0 30.0 41.0 34-35 36.57277665713703 40.0 37.0 41.0 29.0 41.0 36-37 36.432776906249245 40.0 37.0 41.0 28.5 41.0 38-39 36.30481451957071 40.0 37.0 41.0 28.0 41.0 40-41 36.233365040510236 40.0 36.5 41.0 27.5 41.0 42-43 36.06246439498183 40.0 36.0 41.0 27.0 41.0 44-45 35.91573779497672 40.0 36.0 41.0 27.0 41.0 46-47 35.78443058521047 40.0 35.0 41.0 27.0 41.0 48-49 35.64328803205943 40.0 35.0 41.0 27.0 41.0 50-51 35.50124408604336 40.0 35.0 41.0 26.5 41.0 52-53 35.33366564683624 39.5 35.0 41.0 26.0 41.0 54-55 35.171892177679936 39.0 35.0 41.0 26.0 41.0 56-57 34.97390418488846 39.0 34.5 41.0 26.0 41.0 58-59 34.75056378026468 39.0 34.0 41.0 26.0 41.0 60-61 34.506499534225725 38.0 34.0 40.5 25.0 41.0 62-63 34.39627508412454 38.0 34.0 40.5 25.0 41.0 64-65 34.141142061482206 38.0 34.0 40.0 24.5 41.0 66-67 33.86674873165829 37.0 34.0 40.0 23.0 41.0 68-69 33.56385712890311 37.0 33.0 40.0 23.0 41.0 70-71 33.23009970388425 36.0 33.0 39.5 22.0 41.0 72-73 32.89155965221313 36.0 33.0 39.0 21.0 41.0 74-75 32.60605837616862 35.0 33.0 39.0 20.0 41.0 76-77 32.34798287943559 35.0 33.0 38.5 20.0 40.0 78-79 32.01412089284438 35.0 32.5 37.0 18.5 39.5 80-81 31.700678404659186 35.0 32.0 37.0 18.0 39.0 82-83 31.390429141670772 35.0 32.0 36.5 16.0 39.0 84-85 31.101697437487587 35.0 32.0 36.0 12.0 37.5 86-87 30.837226148653123 35.0 32.0 36.0 7.5 37.0 88-89 30.581532295759125 35.0 31.5 35.0 4.5 37.0 90-91 30.361631934087534 35.0 31.0 35.0 2.0 36.0 92-93 30.141430671088628 35.0 31.0 35.0 2.0 36.0 94-95 29.93720717023607 35.0 31.0 35.0 2.0 36.0 96-97 29.748255886750968 35.0 31.0 35.0 2.0 36.0 98-99 29.541471119044825 35.0 31.0 35.0 2.0 35.0 100 29.376928734896754 35.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22631178892256187 1101 2 0.2621933943562347 1101 3 0.3082673852201836 1101 4 0.4035924255691228 1101 5 0.2968763135232706 1101 6 0.31042930272596436 1101 7 0.33579744933215494 1101 8 0.3129878547632856 1101 9 0.25817623319520777 1101 10-11 0.27930484804900146 1101 12-13 0.19018415277003697 1101 14-15 0.3281811416940954 1101 16-17 0.3608266181845252 1101 18-19 0.18168740789740667 1101 20-21 0.20629916120276448 1101 22-23 0.2147642430270622 1101 24-25 0.22190097745068726 1101 26-27 0.2424103381273781 1101 28-29 0.2116455050709405 1101 30-31 0.26450030391243473 1101 32-33 0.25600839982458723 1101 34-35 0.28565632357485526 1101 36-37 0.24909565148942647 1101 38-39 0.3363372209352846 1101 40-41 0.1813557480101764 1101 42-43 0.18464083108383988 1101 44-45 0.12520814389175428 1101 46-47 0.21079619188913057 1101 48-49 0.18578960031668856 1101 50-51 0.1637293976061116 1101 52-53 0.19720142656311168 1101 54-55 0.1789770681627445 1101 56-57 0.08835410497263041 1101 58-59 0.2171650655283841 1101 60-61 0.15521056222266338 1101 62-63 0.10521084805444758 1101 64-65 0.10929516934528039 1101 66-67 0.1362235148542439 1101 68-69 0.15210981553411074 1101 70-71 0.1263227247327805 1101 72-73 0.16204081235768086 1101 74-75 0.23118298125672965 1101 76-77 0.22277476076456537 1101 78-79 0.19282794254094426 1101 80-81 0.16534142225856385 1101 82-83 0.12156195791850521 1101 84-85 0.049511409964761555 1101 86-87 0.1395117441024034 1101 88-89 0.09952221384812532 1101 90-91 0.09477080823110384 1101 92-93 0.012287857701700489 1101 94-95 0.0436154829137223 1101 96-97 -0.04620709271081935 1101 98-99 -0.03312701556690811 1101 100 0.012476327569082457 1102 1 0.008689598533187848 1102 2 0.06046494013042292 1102 3 0.12198906773092943 1102 4 0.21481716837417508 1102 5 0.12250480681667142 1102 6 0.11479117276112305 1102 7 -0.06683674782892979 1102 8 0.02042757347665969 1102 9 0.034971440904548956 1102 10-11 0.08799933951923578 1102 12-13 0.04605473487708167 1102 14-15 0.10642950736670542 1102 16-17 0.04283031658275149 1102 18-19 0.032884256445399274 1102 20-21 0.012176749430402367 1102 22-23 0.10440346688433522 1102 24-25 0.07090274851463363 1102 26-27 0.07901085902853566 1102 28-29 0.12922550246643283 1102 30-31 0.12266355433565934 1102 32-33 0.12659287697005084 1102 34-35 0.14374300152771013 1102 36-37 0.11386806977304786 1102 38-39 0.1673790753434119 1102 40-41 0.09523133076835677 1102 42-43 0.040894666218626696 1102 44-45 0.09476582067257766 1102 46-47 -0.0326969712831513 1102 48-49 -0.016198418956655303 1102 50-51 -0.07706995126743976 1102 52-53 0.023449167154325323 1102 54-55 -0.0482521804373377 1102 56-57 -0.061332442754938654 1102 58-59 -0.10909147829242727 1102 60-61 -0.11316196024120018 1102 62-63 0.0010100146125964216 1102 64-65 0.006635500117511128 1102 66-67 -0.026908299184036366 1102 68-69 -0.09038178493469928 1102 70-71 -0.1088325298576649 1102 72-73 -0.18787386403247552 1102 74-75 -0.1603573495155004 1102 76-77 -0.21320182472833693 1102 78-79 -0.15193603714391202 1102 80-81 -0.2241158508238641 1102 82-83 -0.2252739582164054 1102 84-85 -0.22638636318278316 1102 86-87 -0.1742570018280567 1102 88-89 -0.17122676388335378 1102 90-91 -0.16311847422754866 1102 92-93 -0.19351516352502074 1102 94-95 -0.19668411338787806 1102 96-97 -0.1374305853530906 1102 98-99 -0.18655029984871518 1102 100 -0.13568155757221234 1103 1 -0.1525720629965832 1103 2 -0.1175680410287292 1103 3 -0.09278607832739638 1103 4 -0.006629332323228709 1103 5 -0.03265391586577948 1103 6 -0.07217492166649464 1103 7 -0.06849417001829039 1103 8 -0.18302232011748032 1103 9 -0.055016408185665 1103 10-11 -0.08865336226215703 1103 12-13 0.018643475958761258 1103 14-15 -0.09753895823777015 1103 16-17 -0.03906327507007035 1103 18-19 0.036994970922620496 1103 20-21 0.0026852615888657283 1103 22-23 -0.05718960662090211 1103 24-25 0.01507090704414793 1103 26-27 0.011398028338597044 1103 28-29 -0.033833123137100074 1103 30-31 -0.04443469495198116 1103 32-33 -0.09467922225126557 1103 34-35 -0.12546264076764402 1103 36-37 -0.1723495449675383 1103 38-39 -0.09304438251691494 1103 40-41 -0.06851043413836067 1103 42-43 -0.1284451557433286 1103 44-45 -0.20292067736648534 1103 46-47 -0.3343748278701213 1103 48-49 -0.2857779672508727 1103 50-51 -0.31800632803550144 1103 52-53 -0.387494280909074 1103 54-55 -0.25376624321587116 1103 56-57 -0.27952597678980595 1103 58-59 -0.22586650385612472 1103 60-61 -0.1939975231736497 1103 62-63 -0.17107859096440592 1103 64-65 -0.19458291377986825 1103 66-67 -0.1437434167832734 1103 68-69 -0.1213573659330649 1103 70-71 -0.18652542425654417 1103 72-73 -0.14627024828279644 1103 74-75 -0.20950800216366616 1103 76-77 -0.23822782837920187 1103 78-79 -0.18170832818356075 1103 80-81 -0.13062423751885888 1103 82-83 -0.16310667710307314 1103 84-85 -0.1583259736373286 1103 86-87 -0.2472941609623298 1103 88-89 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-0.12576302124360694 1104 44-45 -0.12172476140520416 1104 46-47 -0.16891167327940337 1104 48-49 -0.14453463724350257 1104 50-51 -0.17195021716114667 1104 52-53 -0.18020338884620202 1104 54-55 -0.1497722095258638 1104 56-57 -0.1921531181413414 1104 58-59 -0.20413188471238897 1104 60-61 -0.31821030455100896 1104 62-63 -0.28340616639177796 1104 64-65 -0.17621558506884583 1104 66-67 -0.09936300199968429 1104 68-69 -0.08510687788482585 1104 70-71 -0.1629010312864807 1104 72-73 -0.2034288505636752 1104 74-75 -0.14239390477857938 1104 76-77 -0.21242652334458256 1104 78-79 -0.23094605424878267 1104 80-81 -0.14813979764512908 1104 82-83 -0.23920208702531198 1104 84-85 -0.2607903556853195 1104 86-87 -0.2463187534898914 1104 88-89 -0.27276799481961334 1104 90-91 -0.27439708904016413 1104 92-93 -0.3436460106771122 1104 94-95 -0.38562560857905126 1104 96-97 -0.38813710876218366 1104 98-99 -0.29521176677076255 1104 100 -0.26651693085788253 1105 1 -0.10086154696235994 1105 2 -0.029272481317544674 1105 3 0.039208653998290544 1105 4 0.117866453572816 1105 5 0.040538431980834844 1105 6 -0.10320787393974484 1105 7 -0.03193437856310055 1105 8 -0.04357301022570681 1105 9 -0.019649403904907103 1105 10-11 -0.13051622170509347 1105 12-13 -0.04491722827803102 1105 14-15 0.01988255463703581 1105 16-17 -0.02563066501006972 1105 18-19 0.11547043344520347 1105 20-21 -0.010261040372839147 1105 22-23 -0.00941630747847455 1105 24-25 0.03778804407908609 1105 26-27 0.04817606389524798 1105 28-29 0.09682706983348055 1105 30-31 0.09168555572246362 1105 32-33 0.0312925002296538 1105 34-35 0.04180161110714664 1105 36-37 -0.03546095234434432 1105 38-39 0.008896554970299064 1105 40-41 -0.044322203063089205 1105 42-43 -0.09746798146998259 1105 44-45 -0.08402322790680472 1105 46-47 -0.06897250648103181 1105 48-49 -0.2416690798734038 1105 50-51 -0.1794320989014082 1105 52-53 -0.11300159069202209 1105 54-55 -0.1389857679295048 1105 56-57 -0.21508339702079837 1105 58-59 -0.07618383250370186 1105 60-61 -0.21841056494219657 1105 62-63 -0.1329820649044393 1105 64-65 -0.14941548673229477 1105 66-67 -0.23876625964899034 1105 68-69 -0.20554259412364928 1105 70-71 -0.1708172746293286 1105 72-73 -0.1036222374302227 1105 74-75 -0.1379020313417456 1105 76-77 -0.10788432767991907 1105 78-79 -0.10056226573258442 1105 80-81 -0.10347039160294713 1105 82-83 -0.10121723386537695 1105 84-85 -0.09998969040840677 1105 86-87 -0.12026455800585012 1105 88-89 -0.12069928967625998 1105 90-91 -0.16130094247855453 1105 92-93 -0.17882829705222747 1105 94-95 -0.24952517986177014 1105 96-97 -0.1685132962639102 1105 98-99 -0.20164029340315892 1105 100 -0.10784838776916317 1106 1 -0.2541561664444032 1106 2 -0.26855650349069293 1106 3 -0.231246429018384 1106 4 -0.16246987208921837 1106 5 -0.054755558235328294 1106 6 -0.10020316623521808 1106 7 -0.15334540781070416 1106 8 -0.1367416439008693 1106 9 -0.14689860571725433 1106 10-11 -0.15258576958419212 1106 12-13 -0.15880934086504084 1106 14-15 -0.21648923391489916 1106 16-17 -0.20765981903063135 1106 18-19 -0.29128710098204635 1106 20-21 -0.3681707429305021 1106 22-23 -0.19708689637259624 1106 24-25 -0.2596279868743139 1106 26-27 -0.38213016163688707 1106 28-29 -0.40377338538953467 1106 30-31 -0.373107239699074 1106 32-33 -0.405882154945175 1106 34-35 -0.33176200111476106 1106 36-37 -0.40813320282458676 1106 38-39 -0.3832873586655339 1106 40-41 -0.38262614732221323 1106 42-43 -0.3742385654272411 1106 44-45 -0.3903246551024324 1106 46-47 -0.35726101383143316 1106 48-49 -0.3933164817200847 1106 50-51 -0.389476700146524 1106 52-53 -0.4255832976235965 1106 54-55 -0.37634036072300603 1106 56-57 -0.32831979098839525 1106 58-59 -0.47050238693114466 1106 60-61 -0.4756376540275298 1106 62-63 -0.37147788367586543 1106 64-65 -0.39651014261448836 1106 66-67 -0.2852761551127472 1106 68-69 -0.2282918817784747 1106 70-71 -0.34442981337817713 1106 72-73 -0.36845511723528546 1106 74-75 -0.33182666827965335 1106 76-77 -0.2709392734960048 1106 78-79 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34-35 -0.4143008970065267 1107 36-37 -0.4515036334476292 1107 38-39 -0.45342481854863337 1107 40-41 -0.3454387180243259 1107 42-43 -0.43530221259717905 1107 44-45 -0.4834601601654569 1107 46-47 -0.44548672432414094 1107 48-49 -0.4537459196584379 1107 50-51 -0.5080887898647504 1107 52-53 -0.45898809621009207 1107 54-55 -0.42669008557579247 1107 56-57 -0.3853029757051232 1107 58-59 -0.573577667255698 1107 60-61 -0.6653234343574681 1107 62-63 -0.5778027381417274 1107 64-65 -0.45462946923703385 1107 66-67 -0.42764068536338584 1107 68-69 -0.5127799715014802 1107 70-71 -0.42879532283445343 1107 72-73 -0.40665646729411975 1107 74-75 -0.42888019300099955 1107 76-77 -0.4401256589463003 1107 78-79 -0.3761158162030327 1107 80-81 -0.41954973272356 1107 82-83 -0.4142465295678939 1107 84-85 -0.30907525255661383 1107 86-87 -0.4223832349120542 1107 88-89 -0.5236757964114744 1107 90-91 -0.5288501462224957 1107 92-93 -0.46114487974583085 1107 94-95 -0.5592993624150502 1107 96-97 -0.5849868844098722 1107 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2213 94-95 0.35674743560929656 2213 96-97 0.3858001917264673 2213 98-99 0.42254165357972084 2213 100 0.46166684447541684 2214 1 0.21479272044581066 2214 2 0.25083168366167286 2214 3 0.17872445190135977 2214 4 0.1184167692315512 2214 5 0.21515499785550674 2214 6 0.17873189542939372 2214 7 0.10835712504018602 2214 8 0.1609002741663943 2214 9 0.21262853343733923 2214 10-11 0.24852758113384965 2214 12-13 0.2827842452694185 2214 14-15 0.25881785306879834 2214 16-17 0.26035245544000674 2214 18-19 0.2812413649668102 2214 20-21 0.2297333914174189 2214 22-23 0.2840189917823963 2214 24-25 0.31590094480385034 2214 26-27 0.30817368116407096 2214 28-29 0.2854005558095736 2214 30-31 0.30051425499657825 2214 32-33 0.3154802174375604 2214 34-35 0.2773221504921679 2214 36-37 0.3457852398671477 2214 38-39 0.3666656808204749 2214 40-41 0.2946072320812405 2214 42-43 0.3425058799180718 2214 44-45 0.3833218963742695 2214 46-47 0.47665107667298656 2214 48-49 0.5356596114710257 2214 50-51 0.5394933174036964 2214 52-53 0.4123732632200685 2214 54-55 0.507723505576017 2214 56-57 0.546064364242973 2214 58-59 0.5212080505380428 2214 60-61 0.5659393812231315 2214 62-63 0.5362300933682675 2214 64-65 0.5219085817565201 2214 66-67 0.5329622693771938 2214 68-69 0.538562899300473 2214 70-71 0.5454668986316307 2214 72-73 0.5704637413235858 2214 74-75 0.5663472873752937 2214 76-77 0.5911270854918271 2214 78-79 0.5992615252598839 2214 80-81 0.532932379083114 2214 82-83 0.6232698230870426 2214 84-85 0.5625252032558343 2214 86-87 0.5971034518043368 2214 88-89 0.5756037438968207 2214 90-91 0.6116684222910109 2214 92-93 0.6555827076622549 2214 94-95 0.6538621080463507 2214 96-97 0.6409164048542699 2214 98-99 0.6273344149089191 2214 100 0.707050008772665 2215 1 0.08612720323240453 2215 2 0.015098801428052866 2215 3 -0.04349945390803711 2215 4 -0.03355006699180052 2215 5 0.05968891304610224 2215 6 0.10658171942167627 2215 7 0.10764164416903554 2215 8 -0.04920748609401926 2215 9 -0.05289652770873943 2215 10-11 -0.0660325242803097 2215 12-13 -0.0769196619365573 2215 14-15 -0.0847487995974916 2215 16-17 0.054615986331853605 2215 18-19 0.06554337658625542 2215 20-21 1.0630808102973788E-4 2215 22-23 0.04044976966030589 2215 24-25 0.029109169821275316 2215 26-27 -0.04416466120243712 2215 28-29 -0.030457339204318146 2215 30-31 -0.05773063729873229 2215 32-33 -0.04207104686870622 2215 34-35 -0.02050931457758054 2215 36-37 -0.05162770051980203 2215 38-39 -0.04329785399166752 2215 40-41 0.01813561021965171 2215 42-43 0.05429749140606788 2215 44-45 0.09093714155949328 2215 46-47 0.07518275597244894 2215 48-49 0.14604984748179817 2215 50-51 0.16895947561591385 2215 52-53 0.2354483450415188 2215 54-55 0.19328667328833404 2215 56-57 0.22637045754481022 2215 58-59 0.2338375047423895 2215 60-61 0.3428677694177935 2215 62-63 0.2451775424221907 2215 64-65 0.19745603576352266 2215 66-67 0.24296789466283997 2215 68-69 0.23701247751840526 2215 70-71 0.29816706969162965 2215 72-73 0.31865090806043384 2215 74-75 0.31609083317212594 2215 76-77 0.3060599013698777 2215 78-79 0.321226645058168 2215 80-81 0.3851929945181709 2215 82-83 0.3677567411799032 2215 84-85 0.34299965487803874 2215 86-87 0.30096990028286896 2215 88-89 0.3629159140867131 2215 90-91 0.3343130005480788 2215 92-93 0.33280940288573646 2215 94-95 0.28752564563522043 2215 96-97 0.21901215507086036 2215 98-99 0.22034544509309484 2215 100 0.2702180427126386 2216 1 0.1288431090929052 2216 2 -0.0066408810341371804 2216 3 -0.0571768269362245 2216 4 -0.13164711501676152 2216 5 -0.06113798509590396 2216 6 -0.0602986879825167 2216 7 -0.06048181250807971 2216 8 -0.05065937713760604 2216 9 -0.16735707432834346 2216 10-11 -0.1791878606876125 2216 12-13 -0.15878072011678768 2216 14-15 -0.12175366279680588 2216 16-17 -0.11870385026302444 2216 18-19 -0.15325728173996112 2216 20-21 -0.1469717124583525 2216 22-23 -0.19937208424097008 2216 24-25 -0.1750563589798233 2216 26-27 -0.22469994707071095 2216 28-29 -0.22912868514715257 2216 30-31 -0.20555601303168913 2216 32-33 -0.20589646531929873 2216 34-35 -0.1956953863232016 2216 36-37 -0.15131028476041308 2216 38-39 -0.11596599238256289 2216 40-41 -0.12164159358228233 2216 42-43 -0.06969144404843775 2216 44-45 -0.015322195506882963 2216 46-47 -0.015194774687202539 2216 48-49 -0.01814587522854083 2216 50-51 0.026811188804970243 2216 52-53 -0.04180768428990689 2216 54-55 -0.03662370613071886 2216 56-57 0.0335072860282466 2216 58-59 0.06648188060129456 2216 60-61 0.06631320772531524 2216 62-63 0.06622011376615689 2216 64-65 0.07701208765732304 2216 66-67 0.04376317158414622 2216 68-69 0.02176938451075472 2216 70-71 0.053463878430029865 2216 72-73 0.05669447089426427 2216 74-75 0.04544040802147009 2216 76-77 0.06477047634077593 2216 78-79 0.10863313030710486 2216 80-81 0.03089382902072657 2216 82-83 0.05677063000522509 2216 84-85 0.08882435357039142 2216 86-87 0.03785072977797199 2216 88-89 -0.03755591236171085 2216 90-91 0.0051720471006362345 2216 92-93 0.026535063792813673 2216 94-95 -0.0359991254228369 2216 96-97 0.019495423742913687 2216 98-99 -0.019712949235767496 2216 100 0.07524489125471945 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 81819.0 3 17197.0 4 12793.0 5 11264.0 6 7242.0 7 5835.0 8 6257.0 9 6446.0 10 6717.0 11 7235.0 12 8325.0 13 9578.0 14 10641.0 15 10937.0 16 10654.0 17 10316.0 18 9900.0 19 9285.0 20 8392.0 21 9140.0 22 10930.0 23 17975.0 24 28925.0 25 36349.0 26 39634.0 27 29292.0 28 28183.0 29 34538.0 30 40109.0 31 45174.0 32 58138.0 33 78465.0 34 111054.0 35 180316.0 36 296603.0 37 530992.0 38 804974.0 39 417578.0 40 1632.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.42798667401308 22.070197190720144 14.032575510313979 21.4692406249528 2 14.635937806794445 23.146723509212944 39.956842743283275 22.260495940709337 3 17.16961782061104 30.275486644032085 30.23824762502738 22.316647910329493 4 11.50849666506758 20.07185053040743 38.940314675245915 29.479338129279075 5 11.725101154612457 41.778817119094306 33.92558711710094 12.570494609192304 6 26.671252540600403 41.20847291054069 18.878645572958696 13.241628975900216 7 24.322456287449505 36.795926307177645 22.635362766111694 16.246254639261156 8 23.977681754759075 37.91231714973009 20.742841623901292 17.367159471609543 9 25.098327836420264 19.693511018752478 21.073249318461492 34.134911826365766 10-11 22.100994561402356 30.43846643355267 28.572646937262597 18.887892067782378 12-13 22.96616950819312 28.81165626935811 28.07455359800671 20.147620624442062 14-15 20.988011288239964 30.06055612310919 25.79445162701837 23.15698096163248 16-17 19.956629143424763 32.96936209614108 26.61270920583576 20.46129955459839 18-19 20.173354282359192 31.76732804796846 28.351035041509903 19.708282628162443 20-21 22.177013960802423 30.56064622301267 27.531426648301995 19.730913167882903 22-23 21.634008012972163 30.585090636722796 27.709847683334583 20.07105366697046 24-25 21.103765698748365 30.735474788273855 27.62476217248028 20.535997340497502 26-27 20.608534093995484 30.886690555446943 27.72619442807977 20.7785809224778 28-29 20.669177502326438 30.773508825045027 27.951639625762592 20.605674046865943 30-31 20.880896041316387 31.05519463745075 27.625857580288905 20.43805174094396 32-33 20.590168688040947 30.84985957424196 28.010543248574916 20.54942848914218 34-35 21.065347464796265 30.583846217178905 27.786063882863377 20.564742435161456 36-37 20.99334207060128 30.603937675613345 27.608788007525643 20.793932246259732 38-39 20.83369554627343 30.649644353365428 27.854661688740062 20.66199841162108 40-41 20.715803761711683 30.459095592376595 27.985353079930555 20.839747565981174 42-43 20.67287103307933 30.49899711147767 27.9951299753038 20.8330018801392 44-45 20.86279541502044 30.55453398103163 27.863799638269665 20.718870965678267 46-47 20.96299675094143 30.728969074650603 27.692003878072637 20.61603029633533 48-49 20.91997205624902 30.449601437407132 27.73318868281366 20.897237823530194 50-51 20.8069237058852 30.4810033567147 27.738174613133094 20.973898324267005 52-53 20.855296404483216 30.568445313900583 27.71089099881626 20.86536728279994 54-55 20.917565390713893 30.727513364199215 27.677617018652224 20.677304226434664 56-57 20.932359772329875 30.508357543061276 27.463090039850307 21.09619264475854 58-59 21.021659435919727 30.327720961511652 27.68366233915347 20.966957263415154 60-61 21.037026418293028 30.596706225983443 27.479778035689336 20.886489320034197 62-63 20.814683792181338 30.556677630472983 27.678403761643406 20.95023481570227 64-65 20.93218886513464 30.522071359511116 27.62180065948791 20.923939115866336 66-67 20.767587135307053 30.469344536756644 27.605317471147334 21.157750856788972 68-69 21.094726359412117 30.737323666927548 27.34555869940439 20.822391274255946 70-71 21.041684023529676 30.444172784482536 27.415353722481306 21.098789469506478 72-73 20.918806043483603 30.545202225987268 27.62597601781288 20.910015712716245 74-75 20.87120171550639 30.734412221752983 27.537502101331324 20.856883961409306 76-77 20.96569783833777 30.543965651422226 27.44090925716018 21.049427253079823 78-79 21.04218073333481 30.525690827697566 27.66261317667165 20.769515262295982 80-81 20.924868688860133 30.600879579200758 27.556403143364715 20.91784858857439 82-83 20.865857983177012 30.598567280631457 27.750055850270755 20.78551888592078 84-85 20.811194500509714 30.529667115826697 27.58843709531515 21.070701288348438 86-87 21.02294040624938 30.461765415512442 27.525584335512566 20.989709842725617 88-89 20.93463756829158 30.364370477435816 27.66475764075102 21.036234313521586 90-91 20.85598397709739 30.46754405978376 27.50025300269503 21.176218960423817 92-93 20.945773517396834 30.41840048965807 27.498377974833517 21.137448018111577 94-95 20.83293493811029 30.337461391197756 27.72084530090716 21.108758369784788 96-97 20.85233734127246 30.62227527217348 27.476097181125457 21.049290205428612 98-99 20.70629244116157 30.58015625515125 27.505538540318458 21.208012763368725 100 20.76098855945145 30.727483463153415 27.293055262576875 21.218472714818255 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 27208.0 1 20360.5 2 11603.0 3 8020.0 4 5274.0 5 3620.5 6 2838.5 7 2693.0 8 2973.5 9 3037.5 10 2393.0 11 1745.0 12 1596.0 13 1568.5 14 1579.0 15 1697.5 16 1863.5 17 2177.0 18 2634.0 19 3373.5 20 4583.5 21 6272.0 22 9163.0 23 14112.0 24 18838.5 25 23234.0 26 29924.5 27 38692.0 28 50629.0 29 61393.0 30 66813.0 31 73264.5 32 81055.5 33 87967.5 34 95241.0 35 100185.0 36 101383.5 37 100930.5 38 100850.0 39 100642.0 40 100949.0 41 98968.0 42 94217.5 43 91662.0 44 90938.0 45 89507.0 46 87978.5 47 86590.5 48 84866.5 49 83772.5 50 83931.5 51 81136.0 52 75093.5 53 70098.0 54 67245.5 55 66157.5 56 61018.0 57 53950.0 58 49590.0 59 45481.5 60 40938.5 61 35507.0 62 30240.0 63 24638.0 64 19196.0 65 15859.5 66 13161.5 67 10335.0 68 7916.0 69 6146.0 70 4782.5 71 3800.0 72 3046.0 73 2467.5 74 2199.0 75 1842.0 76 1319.0 77 1051.5 78 865.5 79 647.0 80 435.5 81 278.5 82 197.5 83 148.0 84 126.0 85 113.0 86 93.0 87 80.5 88 64.5 89 58.0 90 53.0 91 37.5 92 35.0 93 33.5 94 23.5 95 19.0 96 15.0 97 13.5 98 18.0 99 20.5 100 24.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17555855218605798 2 0.17136297812335904 3 0.18490026006003604 4 0.1751979950400448 5 0.18824360814124924 6 0.07676589417844433 7 0.07853590198614543 8 0.06647362655588603 9 0.060639156374945345 10-11 0.07388143701033881 12-13 0.07239004154273881 14-15 0.0712755921823344 16-17 0.06596556875923108 18-19 0.07093142399750363 20-21 0.07299643310648826 22-23 0.07055447789030803 24-25 0.07025947658902451 26-27 0.0741436603892575 28-29 0.07148864867770582 30-31 0.07730672989746411 32-33 0.0736847694761498 34-35 0.07286532141702891 36-37 0.08486204100255865 38-39 0.0800764643372927 40-41 0.07456977338000036 42-43 0.078322845490774 44-45 0.08872983584160922 46-47 0.07907673770516521 48-49 0.08415731567171468 50-51 0.08084674551286632 52-53 0.07999451953138059 54-55 0.08143674811543336 56-57 0.07540561040030366 58-59 0.08223980721337182 60-61 0.07919146043344213 62-63 0.07101336880341573 64-65 0.07396338181625091 66-67 0.07348810194196079 68-69 0.0721114292026377 70-71 0.06844030189777615 72-73 0.06652279343943329 74-75 0.0711772584152399 76-77 0.07615950261469487 78-79 0.07298004414530584 80-81 0.08012563122083995 82-83 0.08210869552391248 84-85 0.08363286891387732 86-87 0.07983062991955642 88-89 0.08220702929100698 90-91 0.08022396498793445 92-93 0.07132475906588165 94-95 0.07029225451138935 96-97 0.06998086424892341 98-99 0.07940451692881356 100 0.08656649296553008 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3050834.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.235756603372124 #Duplication Level Percentage of deduplicated Percentage of total 1 75.0735897234639 43.71967298477063 2 14.70988536421367 17.132826074677066 3 4.917221141948634 8.590742807624284 4 1.973675941710867 4.597540470216213 5 1.0162007704073328 2.9589610362800336 6 0.5806599827840383 2.0289104056037703 7 0.3605683459666903 1.4698579304217656 8 0.24085527543577975 1.122111135753298 9 0.17670249312306407 0.9261363042651417 >10 0.8575774762413594 8.93290588864453 >50 0.05654641682966485 2.2600091478043147 >100 0.03348154444479195 3.6932763515697533 >500 0.001967468890534789 0.7637705447239459 >1k 0.001011841142828715 0.8293413470023893 >5k 0.0 0.0 >10k+ 5.6213396823817504E-5 0.9739375706429338 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 29751 0.9751759682762157 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.277792236483532E-5 0.0 4 0.0 0.0 0.0 3.277792236483532E-5 0.0 5 0.0 0.0 0.0 3.277792236483532E-5 0.0 6 0.0 0.0 0.0 3.277792236483532E-5 0.0 7 0.0 0.0 0.0 3.277792236483532E-5 0.0 8 0.0 0.0 0.0 3.277792236483532E-5 0.0 9 0.0 0.0 0.0 6.555584472967064E-5 0.0 10-11 0.0 0.0 0.0 9.833376709450597E-5 0.0 12-13 0.0 0.0 0.0 1.311116894593413E-4 0.0 14-15 0.0 0.0 0.0 1.311116894593413E-4 0.0 16-17 0.0 0.0 0.0 1.4750065064175896E-4 0.0 18-19 0.0 0.0 0.0 2.458344177362649E-4 0.0 20-21 0.0 0.0 0.0 3.769461071956062E-4 0.0 22-23 0.0 0.0 0.0 7.047253308439594E-4 0.0 24-25 0.0 0.0 0.0 9.997266321274774E-4 0.0 26-27 0.0 0.0 0.0 0.0016061181958769307 0.0 28-29 0.0 0.0 0.0 0.0029500130128351787 0.0 30-31 0.0 0.0 0.0 0.006834196813068164 0.0 32-33 0.0 0.0 0.0 0.013291447518940722 0.0 34-35 0.0 0.0 0.0 0.023944272287512204 0.0 36-37 0.0 0.0 0.0 0.041578794519793606 0.0 38-39 0.0 0.0 0.0 0.06649001551706844 0.0 40-41 0.0 0.0 0.0 0.09253207483593011 0.0 42-43 0.0 0.0 0.0 0.11945913805864232 0.0 44-45 0.0 0.0 0.0 0.1540398461535436 0.0 46-47 0.0 0.0 0.0 0.19494669326485808 0.0 48-49 0.0 0.0 0.0 0.24085217353680993 0.0 50-51 0.0 0.0 0.0 0.2911662843668322 0.0 52-53 0.0 0.0 0.0 0.33895649517476206 0.0 54-55 0.0 0.0 0.0 0.39343340214511835 0.0 56-57 0.0 0.0 0.0 0.4590220247971538 0.0 58-59 0.0 0.0 0.0 0.5336409650607015 0.0 60-61 0.0 0.0 0.0 0.6174868904699502 0.0 62-63 0.0 0.0 0.0 0.7033650470658187 0.0 64-65 0.0 0.0 0.0 0.7948154504637093 0.0 66-67 0.0 0.0 0.0 0.9019828676355384 0.0 68-69 0.0 1.638896118241766E-5 0.0 1.029964265509038 0.0 70-71 0.0 3.277792236483532E-5 0.0 1.173547954428199 0.0 72-73 0.0 6.555584472967064E-5 0.0 1.3233102817131317 0.0 74-75 0.0 6.555584472967064E-5 0.0 1.478054853197519 0.0 76-77 0.0 6.555584472967064E-5 0.0 1.645173090374632 0.0 78-79 0.0 6.555584472967064E-5 0.0 1.842594516778035 0.0 80-81 0.0 6.555584472967064E-5 0.0 2.066566060296955 0.0 82-83 0.0 6.555584472967064E-5 0.0 2.2980109701150573 0.0 84-85 0.0 6.555584472967064E-5 0.0 2.5368309124652475 0.0 86-87 0.0 6.555584472967064E-5 0.0 2.795333997195521 0.0 88 0.0 6.555584472967064E-5 0.0 3.0085871601011394 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5620 0.0 46.302483 1 GTATCAA 9620 0.0 37.22417 1 TCAACGC 11150 0.0 30.720562 4 ATCAACG 11200 0.0 30.669956 3 TATCAAC 12335 0.0 28.648975 2 CAACGCA 12045 0.0 28.437881 5 GTACATG 20135 0.0 27.833998 1 TACATGG 20120 0.0 26.872023 2 AACGCAG 12995 0.0 26.578764 6 CATGGGT 9020 0.0 26.499022 4 ACATGGG 20400 0.0 25.303743 3 CGCAGAG 14025 0.0 24.024496 8 ACGCAGA 14525 0.0 23.553377 7 ATGGGTG 4870 0.0 23.187555 5 TACCTGG 2180 0.0 22.44839 2 GCAGAGT 15320 0.0 21.288536 9 GTACCTG 2625 0.0 20.43577 1 ATGGGTA 3730 0.0 20.309053 5 CATGGGG 8345 0.0 19.452051 4 GTGGTAT 2855 0.0 18.789455 1 >>END_MODULE