FastQCFastQC Report
Wed 25 May 2016
SRR2431398_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431398_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3050834
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325241.0660691469939039No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269380.8829716726639338No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170340.5583391295626049No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84410.27667844268157493No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54920.18001634962767557No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54250.17782022882923162No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52270.1713302002009942No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43340.1420595155291963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106800.063.5824621
GTATCAA212250.045.109861
GTGGTAT34400.035.543181
ATCAACG272250.034.9057583
TCAACGC274700.034.5938724
TATCAAC275550.034.5565532
CAACGCA283250.033.5828485
TGGTATC35700.033.3240742
AACGCAG295000.032.3732836
TATAACG2750.030.7783662
CGCAGAG330800.028.5998218
ACGCAGA333250.028.5729037
GTACATG191400.026.9283661
GCAGAGT351100.026.8123689
TACATGG190350.026.5806262
CATGGGT86450.025.9444774
ACATGGG190600.025.7805423
GAGTACT249800.023.81876412-13
ATGGGTG44350.023.1128985
CAGAGTA349950.022.68977210-11
Produced by FastQC (version 0.11.5)