##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431398_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3050834 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.63767710730902 34.0 31.0 34.0 31.0 34.0 2 32.765876478366245 34.0 31.0 34.0 31.0 34.0 3 32.85474398148178 34.0 31.0 34.0 31.0 34.0 4 36.32008821194467 37.0 37.0 37.0 35.0 37.0 5 36.23567162290705 37.0 37.0 37.0 35.0 37.0 6 36.28136044111217 37.0 37.0 37.0 35.0 37.0 7 36.235380227177224 37.0 37.0 37.0 35.0 37.0 8 36.256449220114895 37.0 37.0 37.0 35.0 37.0 9 38.047216597166546 39.0 39.0 39.0 35.0 39.0 10-11 38.028200977175416 39.0 39.0 39.0 35.0 39.0 12-13 38.00895361727318 39.0 39.0 39.0 35.0 39.0 14-15 39.45438230988641 41.0 39.0 41.0 36.0 41.0 16-17 39.48464616560587 41.0 39.0 41.0 36.5 41.0 18-19 39.5677018153069 41.0 40.0 41.0 37.0 41.0 20-21 39.56620648648861 41.0 40.0 41.0 37.0 41.0 22-23 39.49539109633628 41.0 39.5 41.0 36.0 41.0 24-25 39.43589310332847 41.0 39.0 41.0 36.0 41.0 26-27 39.3102422157351 41.0 39.0 41.0 35.5 41.0 28-29 39.22115313386438 41.0 39.0 41.0 35.0 41.0 30-31 39.005531602178294 41.0 38.5 41.0 35.0 41.0 32-33 38.73754668395593 41.0 38.0 41.0 35.0 41.0 34-35 38.57952792580652 41.0 38.0 41.0 35.0 41.0 36-37 38.42769354215929 41.0 38.0 41.0 35.0 41.0 38-39 38.27254252443758 40.0 38.0 41.0 35.0 41.0 40-41 38.11897861371678 40.0 38.0 41.0 34.0 41.0 42-43 37.9219387223297 40.0 38.0 41.0 34.0 41.0 44-45 37.63563815664831 40.0 37.0 41.0 33.0 41.0 46-47 37.204120086507494 40.0 37.0 41.0 32.5 41.0 48-49 36.821044180050436 40.0 37.0 41.0 31.0 41.0 50-51 36.637505023216605 40.0 36.5 41.0 31.0 41.0 52-53 36.5721440432354 40.0 36.0 41.0 31.0 41.0 54-55 36.427312007142966 40.0 36.0 41.0 31.0 41.0 56-57 36.26748243267251 40.0 35.0 41.0 30.5 41.0 58-59 36.0830731858895 39.5 35.0 41.0 30.0 41.0 60-61 35.92453637267711 39.0 35.0 41.0 30.0 41.0 62-63 35.75951739753785 39.0 35.0 41.0 30.0 41.0 64-65 35.52892323869473 39.0 35.0 41.0 29.0 41.0 66-67 35.2610815599931 38.0 35.0 41.0 29.0 41.0 68-69 34.97000803714656 37.0 35.0 40.5 29.0 41.0 70-71 34.68055079365183 37.0 35.0 40.0 28.5 41.0 72-73 34.36842138903657 36.0 35.0 39.0 28.0 41.0 74-75 34.045657351399655 36.0 35.0 39.0 28.0 41.0 76-77 33.72036318593538 35.5 34.5 39.0 27.5 40.5 78-79 33.411040227033 35.0 34.0 37.5 27.0 39.5 80-81 33.15392889288634 35.0 34.0 37.0 27.0 39.0 82-83 32.96319875155449 35.0 34.0 37.0 27.5 39.0 84-85 32.70781628892296 35.0 34.0 36.0 27.0 38.0 86-87 32.47667064153605 35.0 34.0 36.0 27.0 37.0 88-89 32.25461349257285 35.0 34.0 36.0 26.0 37.0 90-91 32.0757007755912 35.0 34.0 35.0 26.0 36.5 92-93 31.920457651907643 35.0 34.0 35.0 26.0 36.0 94-95 31.783467897630615 35.0 34.0 35.0 25.5 36.0 96-97 31.642265852550484 35.0 34.0 35.0 25.0 36.0 98-99 31.530298272537934 35.0 34.0 35.0 25.0 36.0 100 31.428837491649823 35.0 34.0 35.0 24.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0065403348318398 1101 2 0.03554135493617139 1101 3 0.03768749911549918 1101 4 0.043774301744505806 1101 5 0.04632053184175078 1101 6 0.010600285953920263 1101 7 0.008843366283137755 1101 8 0.03628338480739046 1101 9 0.05331400204033088 1101 10-11 0.08022878316882753 1101 12-13 0.048653272946936 1101 14-15 0.04947390262281459 1101 16-17 0.025220317244134094 1101 18-19 0.009538217371172664 1101 20-21 0.03794668056222861 1101 22-23 0.04529110124697411 1101 24-25 0.048975894181012336 1101 26-27 0.056357230702850813 1101 28-29 0.014589988862475423 1101 30-31 0.002290220978842683 1101 32-33 0.04815295904079164 1101 34-35 0.0944727774671037 1101 36-37 0.1387806117091941 1101 38-39 0.0646082300682238 1101 40-41 0.08266960459611283 1101 42-43 0.07064246809152763 1101 44-45 0.05370797804782512 1101 46-47 0.0954830468736958 1101 48-49 0.15703113319358408 1101 50-51 0.09566734153776224 1101 52-53 0.17949404714560302 1101 54-55 0.14832479272099874 1101 56-57 0.1468062301297408 1101 58-59 0.06676590058783205 1101 60-61 0.12609038288660201 1101 62-63 0.10900332343404529 1101 64-65 0.09017806680770235 1101 66-67 0.0731284246391013 1101 68-69 0.0570271295936422 1101 70-71 0.13924614020675108 1101 72-73 0.13176381782103874 1101 74-75 0.07433256923162901 1101 76-77 0.004217363558232989 1101 78-79 0.12228572694621676 1101 80-81 0.16275631170000082 1101 82-83 0.1604320861448798 1101 84-85 0.1360842424101918 1101 86-87 0.09447543277774173 1101 88-89 0.08186640648452936 1101 90-91 0.03585483364521025 1101 92-93 0.0836216601198565 1101 94-95 0.07804823823139273 1101 96-97 0.03689158144867477 1101 98-99 0.09819370518852821 1101 100 0.1366733347746809 1102 1 -0.035330646070285354 1102 2 -0.002637939242752907 1102 3 -0.011924742061736993 1102 4 -0.006319304193766584 1102 5 -0.015820684462454437 1102 6 0.0035532746243234214 1102 7 -0.029494594388822293 1102 8 -0.018842543047796312 1102 9 0.0046984010506179175 1102 10-11 -0.042024426884566424 1102 12-13 -0.02251320706348281 1102 14-15 -0.05185759887477559 1102 16-17 -0.04216103449437725 1102 18-19 -0.042323613596408904 1102 20-21 -0.006372788118582662 1102 22-23 0.03341471109200711 1102 24-25 0.030777535019666402 1102 26-27 0.046720949937125056 1102 28-29 0.013867785450571546 1102 30-31 0.0021383522459359483 1102 32-33 0.007652555342403389 1102 34-35 0.020009487976281548 1102 36-37 -0.07975708851218855 1102 38-39 -0.1127753355002099 1102 40-41 -0.07116623340362338 1102 42-43 -0.055003293075294835 1102 44-45 -0.022040895503415925 1102 46-47 -0.05124384464863141 1102 48-49 -0.06621720643396145 1102 50-51 -0.1315566980507299 1102 52-53 -0.10784378330107103 1102 54-55 -0.06191745236299795 1102 56-57 -0.05442103904592699 1102 58-59 -0.08444459557051687 1102 60-61 -0.19667745146682591 1102 62-63 -0.12399573894386862 1102 64-65 -0.1281806517754447 1102 66-67 -0.11555994624252719 1102 68-69 -0.09372169186748636 1102 70-71 -0.06841655024773274 1102 72-73 -0.09475393188595405 1102 74-75 -0.052816642905369804 1102 76-77 -0.08235021034460743 1102 78-79 -0.016483748245867957 1102 80-81 -0.025182013602773168 1102 82-83 -0.025226176649752574 1102 84-85 -0.03085275459462622 1102 86-87 -0.018317639236840932 1102 88-89 -0.1320086814175454 1102 90-91 -0.10385294080458607 1102 92-93 -0.12026293422836787 1102 94-95 -0.15344953491615243 1102 96-97 -0.11052969736367046 1102 98-99 -0.02491706444293129 1102 100 -0.012242669913430149 1103 1 -0.08647347132968264 1103 2 -0.09929434799364856 1103 3 -0.084773731863379 1103 4 -0.028531308066988004 1103 5 -0.025429820190950636 1103 6 -0.05718258935836218 1103 7 -0.08721622944704421 1103 8 -0.036254430318656716 1103 9 -0.06884234590425109 1103 10-11 -0.02279570013801191 1103 12-13 -0.06006278428677092 1103 14-15 -0.07051410555342841 1103 16-17 -0.025509821297767132 1103 18-19 -0.09648131476600952 1103 20-21 -0.12289238371212718 1103 22-23 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0.013614471799243688 1103 86-87 0.007826329893340755 1103 88-89 -0.0025436775490845775 1103 90-91 -0.04359372415628826 1103 92-93 -0.06475753588287247 1103 94-95 -0.03661558189124747 1103 96-97 -0.052055954322391074 1103 98-99 -0.06537277221324089 1103 100 -0.07023831637226507 1104 1 -0.11239844077084626 1104 2 -0.10822365308951021 1104 3 -0.06961394550729239 1104 4 -0.06493452650589404 1104 5 0.0114428432382212 1104 6 -0.015383662681713872 1104 7 -0.07848632874093653 1104 8 -0.10179687201443954 1104 9 -0.08503334948454722 1104 10-11 -0.10074842389377636 1104 12-13 -0.09336727279945478 1104 14-15 -0.10902561583625925 1104 16-17 -0.05140375980241174 1104 18-19 -0.07252277781341832 1104 20-21 -0.037222500818188564 1104 22-23 -0.07168417965191054 1104 24-25 -0.08083534177404061 1104 26-27 -0.09388996030838825 1104 28-29 -0.074574698135919 1104 30-31 0.010164378282212283 1104 32-33 0.018504162893123066 1104 34-35 -0.019673610975914357 1104 36-37 -0.04199306405325132 1104 38-39 -0.010368976994378443 1104 40-41 -0.030275499737754785 1104 42-43 0.03310538943983943 1104 44-45 0.06454714337688472 1104 46-47 0.08017068732313959 1104 48-49 0.15798137396405565 1104 50-51 0.03900474121673625 1104 52-53 0.13224460894335976 1104 54-55 0.21708498533737242 1104 56-57 0.17609932804306538 1104 58-59 0.17910169513037033 1104 60-61 0.1090913459684657 1104 62-63 0.16096544221414177 1104 64-65 0.12184869281091437 1104 66-67 0.1275650217498523 1104 68-69 0.13280176843794322 1104 70-71 0.15927567471717907 1104 72-73 0.185298970309006 1104 74-75 0.1913540780598737 1104 76-77 0.10163502005904235 1104 78-79 0.13533612823032826 1104 80-81 0.12273897622648633 1104 82-83 0.14422406046269742 1104 84-85 0.18460687483394622 1104 86-87 0.1843855451372889 1104 88-89 0.1970644803208046 1104 90-91 0.2377938384606182 1104 92-93 0.214893570232217 1104 94-95 0.2278768096599606 1104 96-97 0.20773267294145015 1104 98-99 0.1758643311917396 1104 100 0.26567493991111846 1105 1 -0.11162072905248976 1105 2 -0.1440179089301239 1105 3 -0.15043219596950053 1105 4 -0.0027731291233550337 1105 5 -0.07455583724573955 1105 6 0.00605054589715337 1105 7 0.02295013756499742 1105 8 0.0291075483643084 1105 9 0.016787532483988343 1105 10-11 -0.05530965972339885 1105 12-13 -0.05778301066229119 1105 14-15 -0.11287060029556528 1105 16-17 -0.1487816857539812 1105 18-19 -0.1319124891003014 1105 20-21 -0.05767525035346921 1105 22-23 -0.11398748772377587 1105 24-25 -0.11028626255040308 1105 26-27 0.010304093841938311 1105 28-29 0.03326553506219909 1105 30-31 -0.044892453725331904 1105 32-33 -0.08462805712990473 1105 34-35 -0.11047460236896 1105 36-37 -0.07991718435497575 1105 38-39 -0.02835285180592706 1105 40-41 -0.11017550751437 1105 42-43 -0.11604402442912232 1105 44-45 -0.13307220885613447 1105 46-47 -0.13050507547504253 1105 48-49 -0.27061113389409286 1105 50-51 -0.2885641131874692 1105 52-53 -0.28784401938710147 1105 54-55 -0.1624239841105677 1105 56-57 -0.12889862745963399 1105 58-59 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-0.08229954804944839 1106 14-15 -0.11045242143816836 1106 16-17 -0.12235170852986954 1106 18-19 -0.11345156732159012 1106 20-21 -0.039121117646161 1106 22-23 -0.0565843662997878 1106 24-25 0.00429030562216326 1106 26-27 -0.048496454665688304 1106 28-29 -0.059874509015884314 1106 30-31 -0.14483321136439287 1106 32-33 -0.04020586541307836 1106 34-35 -0.14014913043797605 1106 36-37 -0.13533097087763934 1106 38-39 -0.11856084112461218 1106 40-41 -0.13605565078656667 1106 42-43 -0.1534112172770108 1106 44-45 -0.1466665622633485 1106 46-47 -0.16011299273026225 1106 48-49 -0.10203345897991767 1106 50-51 -0.0879417736594732 1106 52-53 -0.04023224548365789 1106 54-55 -0.10759134746444232 1106 56-57 -0.06479559290970371 1106 58-59 -0.03968212707207641 1106 60-61 -0.11517040604942963 1106 62-63 -0.1207162428082924 1106 64-65 -0.08609966382250178 1106 66-67 -0.13927704447923617 1106 68-69 -0.1019064551934008 1106 70-71 -0.09820939963294961 1106 72-73 -0.05711966481293729 1106 74-75 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66-67 0.2754054331000688 2214 68-69 0.34806933652021144 2214 70-71 0.2804335494077179 2214 72-73 0.2705379724038437 2214 74-75 0.29668830011281955 2214 76-77 0.24557712632451256 2214 78-79 0.3594153512354623 2214 80-81 0.31201911334322574 2214 82-83 0.28642173709572205 2214 84-85 0.30804241092231166 2214 86-87 0.28404465768728215 2214 88-89 0.2851334195704709 2214 90-91 0.2687403248441669 2214 92-93 0.24648374353254 2214 94-95 0.22568294182526927 2214 96-97 0.2754778643335669 2214 98-99 0.2996350415326283 2214 100 0.3420489639065458 2215 1 0.08195052113148193 2215 2 0.05246863692421755 2215 3 0.03177814061886153 2215 4 -0.012903220170620955 2215 5 -0.024375036879263234 2215 6 0.031085287160259156 2215 7 -0.028276179061741402 2215 8 5.065303989013614E-4 2215 9 -2.1451845400122238E-4 2215 10-11 -0.020201659422916407 2215 12-13 0.007720457891849719 2215 14-15 0.06458616705194231 2215 16-17 -5.686219832696793E-4 2215 18-19 -0.023770202863126144 2215 20-21 -0.04314852357684629 2215 22-23 -0.03099503123567615 2215 24-25 0.03118358793219045 2215 26-27 0.020032967238783783 2215 28-29 0.01532972561287238 2215 30-31 -0.026272586302077627 2215 32-33 -0.03191468163153388 2215 34-35 -0.03270721341835525 2215 36-37 -0.037281152760151315 2215 38-39 -0.05762667865226234 2215 40-41 -0.10935229176610761 2215 42-43 -0.057578361332382144 2215 44-45 -0.018744618258097034 2215 46-47 0.009234069579648008 2215 48-49 0.08785256846689293 2215 50-51 0.0957630764652464 2215 52-53 0.04476197733888654 2215 54-55 0.011800481003440666 2215 56-57 0.09923004590864082 2215 58-59 0.1407714014858783 2215 60-61 0.18386980009165654 2215 62-63 0.2112596138128353 2215 64-65 0.10665847857082866 2215 66-67 0.11410593315047635 2215 68-69 0.12877561094903456 2215 70-71 0.09121307048045679 2215 72-73 0.029764150233987152 2215 74-75 0.051252345120971654 2215 76-77 0.005480082374418771 2215 78-79 0.03871374614038103 2215 80-81 0.05687129440917005 2215 82-83 0.006568493404351727 2215 84-85 0.023180336558112913 2215 86-87 7.07746532668807E-4 2215 88-89 -0.037771091839999826 2215 90-91 -0.02435244190903063 2215 92-93 -0.05921654592255621 2215 94-95 -0.058871173076006755 2215 96-97 -0.05289840863255435 2215 98-99 -0.03278619455143428 2215 100 0.01503078591802165 2216 1 0.08045865858666446 2216 2 0.06505060774298954 2216 3 0.03572409738624316 2216 4 0.020961964668757105 2216 5 0.03927776503313396 2216 6 0.028307861124915235 2216 7 0.05257504779043387 2216 8 0.042365201816039644 2216 9 0.0239998711847349 2216 10-11 0.012455599532231076 2216 12-13 0.0037630419493908107 2216 14-15 -0.023286143834795325 2216 16-17 0.008126705165160786 2216 18-19 -0.05215129095248017 2216 20-21 -0.06304895758462692 2216 22-23 -0.1419269656003621 2216 24-25 -0.05048354574671521 2216 26-27 -0.0677403302727555 2216 28-29 -0.08807936202151012 2216 30-31 -0.1688656959136594 2216 32-33 -0.10099991229743921 2216 34-35 -0.055629377649694334 2216 36-37 -0.13496516193901442 2216 38-39 -0.1570655859080361 2216 40-41 -0.10313016311584278 2216 42-43 -0.041851118177667956 2216 44-45 -0.10092792335880318 2216 46-47 -0.019319482484931427 2216 48-49 -0.06682278344081283 2216 50-51 -0.09411848889197216 2216 52-53 -0.053819051318711786 2216 54-55 -0.007332866331779542 2216 56-57 -0.006211604429552153 2216 58-59 0.007687000084544593 2216 60-61 -0.03476846341614248 2216 62-63 -0.06619461178340202 2216 64-65 -0.03944247777869947 2216 66-67 -0.042386867656681204 2216 68-69 -0.10220518425330738 2216 70-71 -0.07627338489326263 2216 72-73 -0.028384433566230882 2216 74-75 -0.027413823851347274 2216 76-77 0.04097121566818629 2216 78-79 0.04260772978529559 2216 80-81 0.07948745120187084 2216 82-83 0.027120845984860864 2216 84-85 0.04045961987824853 2216 86-87 0.056621774241151 2216 88-89 0.06893044055343722 2216 90-91 0.09453388900719517 2216 92-93 0.13068724277567512 2216 94-95 0.060089998458519034 2216 96-97 0.061502757084241466 2216 98-99 0.07116755987945922 2216 100 0.08496911487533865 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49.0 3 306.0 4 84.0 5 123.0 6 301.0 7 705.0 8 1337.0 9 2162.0 10 3098.0 11 4312.0 12 6625.0 13 9555.0 14 10772.0 15 14302.0 16 18864.0 17 28668.0 18 34671.0 19 17772.0 20 10601.0 21 9044.0 22 8928.0 23 8579.0 24 9251.0 25 10682.0 26 13013.0 27 17023.0 28 20702.0 29 26033.0 30 33197.0 31 41130.0 32 52801.0 33 68179.0 34 97083.0 35 170933.0 36 268258.0 37 531504.0 38 915780.0 39 579018.0 40 5389.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.799585263139264 20.59454358129803 14.777114995727084 21.828756159835628 2 14.54988931986706 21.9269914310992 40.83826474268221 22.684854506351527 3 16.581924144735332 29.15553574296662 32.18443626446747 22.078103847830576 4 10.879975770559788 18.727567609381566 39.771092101372936 30.621364518685713 5 11.061719391489351 39.265411484471116 35.35905696064724 14.313812163392297 6 26.416383192268082 40.363323602660785 18.673647927091412 14.546645277979724 7 23.652942113533545 36.06246685332601 23.439623394783197 16.84496763835725 8 25.23434575594739 34.62135271863366 20.995963726639992 19.148337798778954 9 26.44603177100542 15.538089281874583 22.18103127278067 35.83484767433933 10-11 22.93164754293416 27.520556674011104 29.265964650977406 20.281831132077325 12-13 24.852155181173412 26.17104044336729 29.486609235376292 19.490195140083006 14-15 22.048928931868772 26.772753539770942 28.94556994761758 22.2327475807427 16-17 19.06023074346228 30.136087378074322 29.553869532068937 21.24981234639446 18-19 19.04118677056831 27.18376352171242 34.26864588502685 19.506403822692416 20-21 20.786561969612244 26.019590053080567 34.26618754084949 18.927660436457703 22-23 20.23499811362995 26.157102011155644 34.43028510573997 19.177614769474435 24-25 19.772593143869138 26.36089538212779 34.33168957380963 19.53482190019344 26-27 19.41819795685209 26.611105857534252 34.30948251320992 19.661213672403736 28-29 19.416214412541642 26.464347887082496 34.59030316022301 19.529134540152857 30-31 19.72319308830032 26.691188979250548 34.1991696022095 19.386448330239638 32-33 19.458111649276937 26.56553891125738 34.42434421590835 19.55200522355734 34-35 19.833231256667283 26.467400558834917 34.109990079688636 19.589378104809164 36-37 19.93061495479139 26.454113779608406 33.72166297938427 19.893608286215933 38-39 19.796774949562494 26.64593443369865 33.77239777698017 19.784892839758687 40-41 19.725330348788862 26.523877038647043 33.722265935302495 20.028526677261603 42-43 19.79878173597134 26.631444401851113 33.49767185276466 20.07210200941289 44-45 20.0960263429995 26.767703668789096 33.06345044847639 20.07281953973501 46-47 20.28512910509391 27.14244671570362 32.304896850150705 20.267527329051767 48-49 20.474808572553794 27.13942224565483 31.672763096069446 20.713006085721933 50-51 20.38888844274341 27.422940068476592 31.37749528938662 20.810676199393377 52-53 20.423709452254922 27.62224020647584 31.26698520429118 20.68706513697806 54-55 20.52455594565245 27.78708860502247 31.202231932579195 20.486123516745884 56-57 20.53495364611349 27.52335558673895 30.989678340116868 20.95201242703069 58-59 20.52146972313505 27.208558225093288 31.414018430130003 20.85595362164166 60-61 20.560562634227438 27.35460169031517 31.31260669360612 20.77222898185127 62-63 20.347404016553693 27.293779876389777 31.538749104947733 20.820067002108793 64-65 20.417426511211772 27.25841204659058 31.558447922561662 20.76571351963599 66-67 20.371034429409516 27.24644374532503 31.430283379364642 20.952238445900807 68-69 20.748069539200202 27.478525487851485 31.030956862174374 20.742448110773935 70-71 20.624936082841884 27.286873972408763 31.049906517059 21.038283427690356 72-73 20.53352064857136 27.339802942053282 31.219798163023587 20.90687824635177 74-75 20.465809573991233 27.58082055055735 30.98241700790034 20.970952867551073 76-77 20.543323936386148 27.435223424834064 30.882500683834003 21.138951954945785 78-79 20.55053019621546 27.470606523623527 31.09819065757901 20.88067262258201 80-81 20.447183025879365 27.55613347148248 31.023246620814348 20.973436881823805 82-83 20.325574740571252 27.50161884325352 31.18551771598794 20.98728870018728 84-85 20.284607456047983 27.504930266617084 30.898402500197896 21.312059777137033 86-87 20.394601630472014 27.495867440809263 30.85190212447796 21.25762880424076 88-89 20.283988690799976 27.35046867361099 31.02436892995438 21.341173705634656 90-91 20.19310366981213 27.455502194263037 30.817820854324435 21.533573281600397 92-93 20.267008609707517 27.489408107806458 30.793213125538692 21.450370156947333 94-95 20.13038170335991 27.317240019581675 31.06435779463923 21.488020482419184 96-97 20.134291434045355 27.530743708059536 30.778059395360767 21.556905462534345 98-99 20.006703104897856 27.475994198290625 30.77754377914171 21.73975891766981 100 20.081217215899535 27.575568754873668 30.607890112562774 21.73532391666402 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3542.0 1 2124.0 2 3940.5 3 6555.5 4 3619.0 5 1730.0 6 4033.5 7 10693.0 8 19271.5 9 21427.0 10 13464.0 11 5690.0 12 4160.0 13 4232.0 14 4702.0 15 5119.5 16 5463.5 17 5907.5 18 6412.5 19 7013.5 20 8048.0 21 9586.0 22 12118.0 23 16686.0 24 21217.5 25 25228.5 26 31140.5 27 39469.5 28 50948.5 29 61062.0 30 66072.5 31 72478.0 32 80166.0 33 86533.5 34 92858.0 35 97460.5 36 98599.0 37 98842.0 38 99292.5 39 98756.0 40 99330.5 41 97710.5 42 93541.0 43 91363.5 44 90656.0 45 89100.0 46 87439.5 47 86377.0 48 84488.0 49 83128.5 50 83665.5 51 80435.0 52 73691.5 53 68149.0 54 64554.0 55 63177.5 56 57707.5 57 50554.5 58 47189.5 59 43874.0 60 39353.5 61 33863.0 62 28959.0 63 23641.5 64 18154.0 65 14980.0 66 12200.0 67 9263.5 68 6947.5 69 5200.5 70 3984.0 71 3182.0 72 2577.0 73 2054.5 74 1828.0 75 1495.5 76 976.0 77 771.5 78 629.5 79 377.0 80 185.0 81 83.5 82 34.0 83 21.0 84 16.0 85 11.5 86 6.5 87 4.5 88 2.5 89 1.0 90 0.5 91 1.0 92 1.5 93 0.5 94 0.0 95 0.0 96 0.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007375032532087947 2 0.004949466277090133 3 7.866701367560477E-4 4 0.0 5 0.042283519850637566 6 0.0 7 0.0 8 0.0 9 6.555584472967064E-5 10-11 0.0 12-13 0.0 14-15 3.277792236483532E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.277792236483532E-5 24-25 8.19448059120883E-5 26-27 8.19448059120883E-5 28-29 1.638896118241766E-4 30-31 2.2944545655384724E-4 32-33 0.0012947279334109953 34-35 7.375032532087947E-4 36-37 0.0010652824768571478 38-39 9.505597485802243E-4 40-41 1.8027857300659426E-4 42-43 5.736136413846181E-4 44-45 1.4750065064175896E-4 46-47 1.8027857300659426E-4 48-49 1.4750065064175896E-4 50-51 3.4416818483077086E-4 52-53 5.572246802022004E-4 54-55 8.194480591208831E-4 56-57 0.0021305649537142956 58-59 4.7527987429011215E-4 60-61 0.0013766727393230834 62-63 0.0011308383215868185 64-65 0.001491395467600007 66-67 3.9333506837802387E-4 68-69 2.622233789186826E-4 70-71 3.277792236483532E-4 72-73 1.8027857300659426E-4 74-75 5.408357190197828E-4 76-77 6.719474084791241E-4 78-79 9.669487097626419E-4 80-81 8.194480591208831E-4 82-83 0.0014586175452351718 84-85 0.0011636162439516537 86-87 0.0019994532642549547 88-89 0.0014258396228703364 90-91 0.0012291720886813246 92-93 0.0010980603992219833 94-95 7.375032532087947E-4 96-97 2.130564953714296E-4 98-99 2.950013012835179E-4 100 9.833376709450597E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3050834.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.17975658902176 #Duplication Level Percentage of deduplicated Percentage of total 1 72.0261412266552 38.30332658480029 2 16.371085170307285 17.412206489101706 3 5.516177201378699 8.800468826136914 4 2.245125489496465 4.775809081729213 5 1.119131969371617 2.9757582861087575 6 0.6846219023510977 2.184481567352664 7 0.4146840308880906 1.5436957076788127 8 0.28219909457218373 1.200582332719285 9 0.20471052290684955 0.9797810201457859 >10 1.0216498113099883 9.709723359331491 >50 0.0665810509675949 2.445615806852586 >100 0.04288852526908402 4.246277782874365 >500 0.0032742251840683616 1.1398124444706887 >1k 0.001297334506321083 0.961616136447862 >5k 2.4711133453734916E-4 0.8076984186734092 >10k+ 1.8533350090301184E-4 2.5131461555762096 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32524 1.0660691469939039 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26938 0.8829716726639338 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17034 0.5583391295626049 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8441 0.27667844268157493 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5492 0.18001634962767557 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5425 0.17782022882923162 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5227 0.1713302002009942 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4334 0.1420595155291963 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.555584472967064E-5 0.0 12-13 0.0 0.0 0.0 1.311116894593413E-4 0.0 14-15 0.0 0.0 0.0 1.8027857300659426E-4 0.0 16-17 0.0 0.0 0.0 2.1305649537142957E-4 0.0 18-19 0.0 0.0 0.0 2.950013012835179E-4 0.0 20-21 0.0 0.0 0.0 3.933350683780238E-4 0.0 22-23 0.0 0.0 0.0 7.047253308439594E-4 0.0 24-25 0.0 0.0 0.0 0.0010161155933098949 0.0 26-27 0.0 0.0 0.0 0.0016061181958769307 0.0 28-29 0.0 0.0 0.0 0.0029172350904703437 0.0 30-31 0.0 0.0 0.0 0.006817807851885746 0.0 32-33 0.0 0.0 0.0 0.013438948169582481 0.0 34-35 0.0 0.0 0.0 0.02423927358879572 0.0 36-37 0.0 0.0 0.0 0.04202129647171888 0.0 38-39 0.0 0.0 0.0 0.06714557396436516 0.0 40-41 0.0 0.0 0.0 0.09343346770096309 0.0 42-43 0.0 0.0 0.0 0.12072108806968848 0.0 44-45 0.0 0.0 0.0 0.15564596434942052 0.0 46-47 0.0 0.0 0.0 0.1971100361409372 0.0 48-49 0.0 0.0 0.0 0.24362190797663852 0.0 50-51 0.0 0.0 0.0 0.29485380063287614 0.0 52-53 0.0 0.0 0.0 0.34351262638347413 0.0 54-55 0.0 0.0 0.0 0.39846481322812055 0.0 56-57 0.0 0.0 0.0 0.46490566186164173 0.0 58-59 0.0 1.638896118241766E-5 0.0 0.540917663825695 0.0 60-61 0.0 3.277792236483532E-5 0.0 0.6261238730130843 0.0 62-63 0.0 3.277792236483532E-5 0.0 0.7133131465035463 0.0 64-65 0.0 3.277792236483532E-5 0.0 0.8056485538052873 0.0 66-67 0.0 3.277792236483532E-5 0.0 0.9140779209881627 0.0 68-69 0.0 3.277792236483532E-5 0.0 1.0439932162811874 0.0 70-71 0.0 3.277792236483532E-5 0.0 1.1895763584646035 0.0 72-73 0.0 3.277792236483532E-5 0.0 1.3420592533058175 0.0 74-75 0.0 3.277792236483532E-5 0.0 1.4986885553261828 0.0 76-77 0.0 3.277792236483532E-5 0.0 1.6679537464181924 0.0 78-79 0.0 3.277792236483532E-5 0.0 1.8688987994758155 0.0 80-81 0.0 3.277792236483532E-5 0.0 2.096312024843043 0.0 82-83 0.0 3.277792236483532E-5 0.0 2.3314116730048244 0.0 84-85 0.0 3.277792236483532E-5 0.0 2.574296077728254 0.0 86-87 0.0 3.277792236483532E-5 0.0 2.8371914040554156 0.0 88 0.0 3.277792236483532E-5 0.0 3.0533945799738693 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10680 0.0 63.582462 1 GTATCAA 21225 0.0 45.10986 1 GTGGTAT 3440 0.0 35.54318 1 ATCAACG 27225 0.0 34.905758 3 TCAACGC 27470 0.0 34.593872 4 TATCAAC 27555 0.0 34.556553 2 CAACGCA 28325 0.0 33.582848 5 TGGTATC 3570 0.0 33.324074 2 AACGCAG 29500 0.0 32.373283 6 TATAACG 275 0.0 30.778366 2 CGCAGAG 33080 0.0 28.599821 8 ACGCAGA 33325 0.0 28.572903 7 GTACATG 19140 0.0 26.928366 1 GCAGAGT 35110 0.0 26.812368 9 TACATGG 19035 0.0 26.580626 2 CATGGGT 8645 0.0 25.944477 4 ACATGGG 19060 0.0 25.780542 3 GAGTACT 24980 0.0 23.818764 12-13 ATGGGTG 4435 0.0 23.112898 5 CAGAGTA 34995 0.0 22.689772 10-11 >>END_MODULE