FastQCFastQC Report
Wed 25 May 2016
SRR2431397_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431397_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3918778
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA224110.5718874608360055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56400.044.8024251
GTATCAA101250.038.80591
TCAACGC112400.033.9451374
ATCAACG112700.033.8582423
TATCAAC120800.032.2898642
CAACGCA121900.031.3378945
AACGCAG132900.028.7843446
GTACATG209650.028.4372831
TACATGG210100.027.5459422
CATGGGT103450.026.7864
ACATGGG214650.025.9311223
CGCAGAG144450.025.7992488
ACGCAGA150650.025.20547
ATGGGTG55950.023.375575
GCAGAGT161200.022.185599
ATGGGTA47000.021.2204955
TCTGTCG7750.020.6171478
GTGGTAT31350.019.9627531
TGGTATC29750.019.769772
CATGGGG84850.019.295254
Produced by FastQC (version 0.11.5)