##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431397_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3918778
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.737780246801428	31.0	31.0	34.0	27.0	34.0
2	30.87350291340821	33.0	31.0	34.0	27.0	34.0
3	30.982228133361982	34.0	31.0	34.0	27.0	34.0
4	34.180773190009745	37.0	35.0	37.0	30.0	37.0
5	34.29499017295698	37.0	35.0	37.0	31.0	37.0
6	34.33562477894895	37.0	35.0	37.0	31.0	37.0
7	34.23560405820386	37.0	35.0	37.0	30.0	37.0
8	34.21204390756506	37.0	35.0	37.0	30.0	37.0
9	35.65942393266472	39.0	37.0	39.0	30.0	39.0
10-11	35.678545454731044	39.0	37.0	39.0	30.0	39.0
12-13	35.60949204063103	39.0	37.0	39.0	30.0	39.0
14-15	36.96572183981843	41.0	38.0	41.0	30.0	41.0
16-17	37.002537525728684	41.0	38.0	41.0	30.0	41.0
18-19	37.04595960781652	41.0	38.0	41.0	30.0	41.0
20-21	37.135338490723385	41.0	38.0	41.0	30.0	41.0
22-23	37.11900750693201	40.5	38.0	41.0	30.0	41.0
24-25	37.174365963062975	41.0	38.0	41.0	30.0	41.0
26-27	37.21997239955925	41.0	38.0	41.0	30.0	41.0
28-29	37.18634316616047	40.0	38.0	41.0	30.0	41.0
30-31	37.12225813761331	40.0	38.0	41.0	30.0	41.0
32-33	37.06726510151889	40.0	38.0	41.0	30.0	41.0
34-35	36.98975892484851	40.0	38.0	41.0	30.0	41.0
36-37	36.85865504501659	40.0	38.0	41.0	30.0	41.0
38-39	36.73822183854253	40.0	37.0	41.0	29.5	41.0
40-41	36.678346795863405	40.0	37.0	41.0	29.5	41.0
42-43	36.51838532317983	40.0	37.0	41.0	29.0	41.0
44-45	36.38294845995358	40.0	37.0	41.0	28.5	41.0
46-47	36.26380047555641	40.0	36.0	41.0	28.0	41.0
48-49	36.136456696449756	40.0	36.0	41.0	28.0	41.0
50-51	36.0161755782032	40.0	36.0	41.0	28.0	41.0
52-53	35.86519751820593	40.0	35.0	41.0	28.0	41.0
54-55	35.71334941657833	40.0	35.0	41.0	28.0	41.0
56-57	35.5337434271602	39.0	35.0	41.0	28.0	41.0
58-59	35.32983917435486	39.0	35.0	41.0	27.0	41.0
60-61	35.10364302341189	39.0	35.0	41.0	27.0	41.0
62-63	35.003904278323496	39.0	34.5	41.0	26.5	41.0
64-65	34.75598005296549	38.5	34.0	40.5	26.0	41.0
66-67	34.487395050191665	38.0	34.0	40.0	26.0	41.0
68-69	34.18335728637856	37.0	34.0	40.0	26.0	41.0
70-71	33.84324373567475	37.0	34.0	40.0	25.0	41.0
72-73	33.49053403892744	36.0	33.5	39.0	24.0	41.0
74-75	33.19791271666831	36.0	33.0	39.0	24.0	41.0
76-77	32.91881857048294	35.5	33.0	39.0	23.0	40.5
78-79	32.56374627498674	35.0	33.0	37.5	22.5	39.5
80-81	32.226997676316444	35.0	33.0	37.0	22.0	39.0
82-83	31.891638668993245	35.0	33.0	37.0	20.5	39.0
84-85	31.585048323737656	35.0	32.0	36.0	20.0	38.0
86-87	31.29443783240592	35.0	32.0	36.0	19.5	37.0
88-89	31.02118581353677	35.0	32.0	36.0	18.0	37.0
90-91	30.784252769613385	35.0	32.0	35.0	16.0	36.5
92-93	30.550437661944613	35.0	32.0	35.0	8.5	36.0
94-95	30.340107809118045	35.0	32.0	35.0	2.0	36.0
96-97	30.140821832724384	35.0	31.0	35.0	2.0	36.0
98-99	29.93080738434277	35.0	31.0	35.0	2.0	35.5
100	29.760310739725497	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.36027354857476723
1101	2	0.36750763670980646
1101	3	0.3784072926711879
1101	4	0.5458082171695935
1101	5	0.42167199230031827
1101	6	0.49063706518274586
1101	7	0.4262766559428428
1101	8	0.4311370821441116
1101	9	0.4950197389710027
1101	10-11	0.4949285520663338
1101	12-13	0.40493854253730177
1101	14-15	0.4325102228789177
1101	16-17	0.4449399697677947
1101	18-19	0.34630220563956726
1101	20-21	0.4021491192238358
1101	22-23	0.4058650853102037
1101	24-25	0.4336374645504506
1101	26-27	0.3897095980861849
1101	28-29	0.39158652906871794
1101	30-31	0.43624784663991534
1101	32-33	0.4191126706243793
1101	34-35	0.40689280821394647
1101	36-37	0.3913245190865027
1101	38-39	0.42977423233917733
1101	40-41	0.4208566263540163
1101	42-43	0.39368076435561505
1101	44-45	0.3448034627682546
1101	46-47	0.3602439565723614
1101	48-49	0.36771129681230974
1101	50-51	0.2834223369996707
1101	52-53	0.3125088102171105
1101	54-55	0.31291159804671764
1101	56-57	0.25282084948661065
1101	58-59	0.262309801477393
1101	60-61	0.3017738298537651
1101	62-63	0.3461514075670138
1101	64-65	0.34681871415155285
1101	66-67	0.36457183671239335
1101	68-69	0.3147527296286441
1101	70-71	0.3635605979905492
1101	72-73	0.3393388317293926
1101	74-75	0.38524600801385134
1101	76-77	0.4194408165834105
1101	78-79	0.39627522107382873
1101	80-81	0.3212161859560325
1101	82-83	0.3316051610199935
1101	84-85	0.23972787461917022
1101	86-87	0.3085619801042938
1101	88-89	0.25672739725411375
1101	90-91	0.23634060275485425
1101	92-93	0.28982235566993
1101	94-95	0.17780154898392908
1101	96-97	0.15597757386036548
1101	98-99	0.26937061587588573
1101	100	0.2571985358307316
1102	1	3.090359562030187E-4
1102	2	0.050959000076112204
1102	3	0.028773828917653788
1102	4	0.1947184732927738
1102	5	0.10817281731631567
1102	6	0.06687242463196696
1102	7	0.017826250450966086
1102	8	0.09928307389992597
1102	9	0.1565698323202227
1102	10-11	0.19244794964542677
1102	12-13	0.15232520919899173
1102	14-15	0.1402456466425619
1102	16-17	0.09753821347393199
1102	18-19	0.1545582621427144
1102	20-21	0.08768869548507041
1102	22-23	0.044677510110837204
1102	24-25	0.07961964161739843
1102	26-27	0.10710429907511809
1102	28-29	0.08524763645614541
1102	30-31	0.13196857063871903
1102	32-33	0.0783046512996961
1102	34-35	0.03251561318872831
1102	36-37	0.019091190082271225
1102	38-39	0.031480514577246765
1102	40-41	0.0011325364840004681
1102	42-43	0.011752191185458116
1102	44-45	-0.06385652440368972
1102	46-47	-0.05599003386282675
1102	48-49	-0.09639378972480728
1102	50-51	-0.09746125115312765
1102	52-53	-0.14730698928708819
1102	54-55	-0.20666018667984076
1102	56-57	-0.20032029131944284
1102	58-59	-0.2045091019131391
1102	60-61	-0.21526762695132362
1102	62-63	-0.1491261626713154
1102	64-65	-0.07293185721431428
1102	66-67	-0.1026148997870493
1102	68-69	-0.1046109599891949
1102	70-71	-0.06196012493842318
1102	72-73	-0.0917132272527823
1102	74-75	-0.09368117449680113
1102	76-77	-0.18715505694333245
1102	78-79	-0.15999134890972044
1102	80-81	-0.13146231394296137
1102	82-83	-0.13533411421987296
1102	84-85	-0.15988671465422755
1102	86-87	-0.1981955907112365
1102	88-89	-0.21282070185031188
1102	90-91	-0.22550763211894065
1102	92-93	-0.1603415061662652
1102	94-95	-0.12116258135566227
1102	96-97	-0.15107666123834562
1102	98-99	-0.2128125155325442
1102	100	-0.28393968271192804
1103	1	-0.24119942156114504
1103	2	-0.23874082221441384
1103	3	-0.17803399786455998
1103	4	-0.1562935596759374
1103	5	-0.14298168856332438
1103	6	-0.14631367783430704
1103	7	-0.1975008086599317
1103	8	-0.16560772797824086
1103	9	-0.13995192126783706
1103	10-11	-0.2110456127126028
1103	12-13	-0.18854098295766164
1103	14-15	-0.21218788598349647
1103	16-17	-0.24490898854129028
1103	18-19	-0.24756122250969526
1103	20-21	-0.31535430424990807
1103	22-23	-0.24250649188283546
1103	24-25	-0.19855564203046328
1103	26-27	-0.24054864035038293
1103	28-29	-0.24485363359303136
1103	30-31	-0.25691805711876725
1103	32-33	-0.233008424686183
1103	34-35	-0.2683080484769462
1103	36-37	-0.34572775417313295
1103	38-39	-0.27383451119408875
1103	40-41	-0.22561201034694278
1103	42-43	-0.2937088089025579
1103	44-45	-0.3606738423750997
1103	46-47	-0.451241648650587
1103	48-49	-0.47658600670244056
1103	50-51	-0.5236278837019555
1103	52-53	-0.5457660988014084
1103	54-55	-0.41033410079285915
1103	56-57	-0.4875850914185236
1103	58-59	-0.49807822479276354
1103	60-61	-0.4996935224953276
1103	62-63	-0.46362686221076643
1103	64-65	-0.4718159718710524
1103	66-67	-0.42180277020055
1103	68-69	-0.5454320881575825
1103	70-71	-0.5545226199320012
1103	72-73	-0.552510539681272
1103	74-75	-0.5466412097667401
1103	76-77	-0.5675614993815756
1103	78-79	-0.5792330167383781
1103	80-81	-0.5311319425578453
1103	82-83	-0.4962666835112408
1103	84-85	-0.5192965741925377
1103	86-87	-0.5688041387397682
1103	88-89	-0.6380976899318114
1103	90-91	-0.641788866018036
1103	92-93	-0.6923813621147836
1103	94-95	-0.7017648645565302
1103	96-97	-0.7706191656464689
1103	98-99	-0.7850993618173057
1103	100	-0.7487494732481323
1104	1	-0.262250967599563
1104	2	-0.17692413373922733
1104	3	-0.1048911290057788
1104	4	-0.08621694016382264
1104	5	-0.10400913914530463
1104	6	-0.048648495126236924
1104	7	-0.08652709394475266
1104	8	-0.004127745506657732
1104	9	-0.04635799550167263
1104	10-11	-0.0636277190361696
1104	12-13	-0.03695921753246978
1104	14-15	-0.1400271349543729
1104	16-17	-0.06969972161357418
1104	18-19	-0.11985404871027328
1104	20-21	-0.1870659813352944
1104	22-23	-0.05231194111590298
1104	24-25	-0.0923140038331951
1104	26-27	-0.09689495026312045
1104	28-29	-0.08323259447112008
1104	30-31	-0.08864018148485542
1104	32-33	-0.16985351115931024
1104	34-35	-0.09758789911817445
1104	36-37	-0.18865568874512206
1104	38-39	-0.12849995340109643
1104	40-41	-0.18349865153701472
1104	42-43	-0.19546033498892967
1104	44-45	-0.2124389095326933
1104	46-47	-0.3314166023508349
1104	48-49	-0.34336694368948173
1104	50-51	-0.4178288778766941
1104	52-53	-0.4711375372701738
1104	54-55	-0.3352494117203335
1104	56-57	-0.34241945275122987
1104	58-59	-0.40728840241489195
1104	60-61	-0.3638665562705867
1104	62-63	-0.2915322558718927
1104	64-65	-0.3056141643139938
1104	66-67	-0.2721244509703311
1104	68-69	-0.22407371827610945
1104	70-71	-0.3634105812617605
1104	72-73	-0.3001368128479811
1104	74-75	-0.217084907063672
1104	76-77	-0.23082628684337436
1104	78-79	-0.24204732259705253
1104	80-81	-0.25505124266960166
1104	82-83	-0.25888711489151817
1104	84-85	-0.27023806880854906
1104	86-87	-0.2494017115677778
1104	88-89	-0.3166133957383437
1104	90-91	-0.29526830929487247
1104	92-93	-0.3382598272519566
1104	94-95	-0.39178812459773127
1104	96-97	-0.4028017017345711
1104	98-99	-0.38141386474829986
1104	100	-0.35223805101029626
1105	1	-0.10334166360715713
1105	2	-0.05187740069387914
1105	3	-0.01435759744039089
1105	4	0.11197935799784631
1105	5	2.501057649126892E-4
1105	6	0.03907475807485383
1105	7	0.053871565567483515
1105	8	0.07140568920267754
1105	9	0.011054882479072603
1105	10-11	-0.04197131103973817
1105	12-13	0.03184151082504627
1105	14-15	0.052413129075233655
1105	16-17	0.05474008719257739
1105	18-19	0.05496047194912279
1105	20-21	0.05129785358325023
1105	22-23	0.04544632554818406
1105	24-25	0.001465052982567272
1105	26-27	0.008203431272235662
1105	28-29	-0.037486754438575076
1105	30-31	-0.025019678027092596
1105	32-33	0.08671369241273652
1105	34-35	-0.023212257446822093
1105	36-37	-0.030012738897184477
1105	38-39	0.004874463041183219
1105	40-41	-0.09420693563519222
1105	42-43	-0.1341119534535693
1105	44-45	-0.1551450434265007
1105	46-47	-0.13119431771026768
1105	48-49	-0.21565848525042242
1105	50-51	-0.1063784342759746
1105	52-53	-0.058771925043060946
1105	54-55	-0.14174687893455484
1105	56-57	-0.22985879664832964
1105	58-59	-0.08042586052464884
1105	60-61	-0.1953527639687067
1105	62-63	-0.05929335557551241
1105	64-65	-0.11593490313197208
1105	66-67	-0.10359988343260795
1105	68-69	-0.043262960474351075
1105	70-71	-0.019905627074678023
1105	72-73	0.008032995183327785
1105	74-75	-0.020142168361928725
1105	76-77	-0.08234184192540539
1105	78-79	-0.09917423308268525
1105	80-81	-0.07967168801373248
1105	82-83	-0.05984005737839482
1105	84-85	-0.0401532471205428
1105	86-87	-0.0390539741251672
1105	88-89	-0.04711578808284145
1105	90-91	-0.13323999827071376
1105	92-93	-0.11314372105897874
1105	94-95	-0.17335375425892963
1105	96-97	-0.137786228980179
1105	98-99	-0.1817874869709506
1105	100	-0.23153894402310016
1106	1	-0.18122136647840392
1106	2	-0.12743384782981693
1106	3	-0.09098793318498366
1106	4	-0.07783890754875955
1106	5	-0.1040640943630251
1106	6	-0.02081601902316521
1106	7	-0.04729529319210002
1106	8	-0.11064678597788458
1106	9	-0.052341296703694695
1106	10-11	-0.10065431570163241
1106	12-13	-0.025015231542198535
1106	14-15	-0.025683823128858307
1106	16-17	-0.07860694274103963
1106	18-19	-0.14765492704523098
1106	20-21	-0.13510668258047076
1106	22-23	-0.010627074609061538
1106	24-25	-0.019029857494622604
1106	26-27	-0.09706574420299319
1106	28-29	-0.10277439126367938
1106	30-31	-0.055453928944587005
1106	32-33	-0.09848268971219198
1106	34-35	-0.020026256793052255
1106	36-37	-0.1229001701030299
1106	38-39	-0.13772229260818136
1106	40-41	-0.1516436846998559
1106	42-43	-0.11596005398813958
1106	44-45	-0.1517118229536223
1106	46-47	-0.13147552952064245
1106	48-49	-0.16819060680155218
1106	50-51	-0.20623196343135675
1106	52-53	-0.2585756755984434
1106	54-55	-0.19411993675311834
1106	56-57	-0.26132730820536665
1106	58-59	-0.3188094644318866
1106	60-61	-0.33278232598747337
1106	62-63	-0.297916635310699
1106	64-65	-0.24033913330906387
1106	66-67	-0.12322462241638021
1106	68-69	-0.15359400724451433
1106	70-71	-0.18009159438224742
1106	72-73	-0.27211578758636534
1106	74-75	-0.2565918099607032
1106	76-77	-0.2372388256440061
1106	78-79	-0.22761499754672343
1106	80-81	-0.2276126715764022
1106	82-83	-0.23949539267680464
1106	84-85	-0.20362757265837672
1106	86-87	-0.2802523673548549
1106	88-89	-0.3489275083805339
1106	90-91	-0.2640929612451579
1106	92-93	-0.21341977242716226
1106	94-95	-0.33268366937171123
1106	96-97	-0.3165320583565894
1106	98-99	-0.3615297983230299
1106	100	-0.4314896996668267
1107	1	-0.217141557811253
1107	2	-0.22718016381177009
1107	3	-0.19467743003061244
1107	4	-0.13199814672484678
1107	5	-0.0970451805758401
1107	6	-0.16117804848148154
1107	7	-0.1291509923025984
1107	8	-0.12199014983695378
1107	9	-0.1117541352097362
1107	10-11	-0.10984055309304352
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2216	56-57	0.25790036142613104
2216	58-59	0.27889313466580035
2216	60-61	0.28400484242944657
2216	62-63	0.22609389442350647
2216	64-65	0.23006928129539972
2216	66-67	0.1622754227807448
2216	68-69	0.1739194909124464
2216	70-71	0.10834270071718777
2216	72-73	0.13389923220709932
2216	74-75	0.18234216445437568
2216	76-77	0.19307113097153916
2216	78-79	0.21544537863683644
2216	80-81	0.15513781016754535
2216	82-83	0.14140064389412998
2216	84-85	0.10245184705837573
2216	86-87	0.12750965280832105
2216	88-89	0.11212907124460259
2216	90-91	0.1517911016749771
2216	92-93	0.10677281993161003
2216	94-95	0.07291161501385446
2216	96-97	0.046823620052247605
2216	98-99	0.11380445712728715
2216	100	0.1175295227417621
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	89433.0
3	17888.0
4	12631.0
5	10862.0
6	7469.0
7	6266.0
8	7275.0
9	7541.0
10	7872.0
11	8507.0
12	9979.0
13	11311.0
14	12686.0
15	13601.0
16	13750.0
17	13323.0
18	12597.0
19	11775.0
20	10800.0
21	11495.0
22	13426.0
23	19907.0
24	32105.0
25	42405.0
26	46189.0
27	35078.0
28	34620.0
29	43489.0
30	50702.0
31	56692.0
32	72960.0
33	96400.0
34	135832.0
35	212258.0
36	353831.0
37	668527.0
38	1101796.0
39	602896.0
40	2604.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.11563466534544	21.846512077383608	14.250346284289048	21.7875069729819
2	14.494111102703434	22.746262048260533	40.68104243400938	22.078584415026658
3	16.992499004145518	29.320512761510876	30.95366298034319	22.73332525400042
4	11.441086572158122	19.590735667516444	40.16723062968542	28.800947130640015
5	11.622472277832749	40.830603943105956	35.07592434922899	12.4709994298323
6	26.651168064741693	41.218559912065054	19.263938143160907	12.866333880032343
7	24.02239787513887	35.963862037261684	23.526452222548556	16.487287865050888
8	23.458480229252114	37.57657612106931	21.667843876898026	17.297099772780548
9	24.550236908252824	18.92740669471366	21.97475974673951	34.547596650294004
10-11	21.591614433942496	29.76766647636576	29.80859117275368	18.83212791693806
12-13	22.673579938063007	28.02844969684634	29.17594276554947	20.122027599541187
14-15	20.66231000245158	29.56509969763831	26.72684532565171	23.045744974258398
16-17	19.597837181349036	32.5531837198203	27.422694733734748	20.426284365095924
18-19	19.670265447831888	31.419245513675115	29.27543974545283	19.63504929304016
20-21	21.680775450205886	30.31569318273522	28.407495957366173	19.596035409692718
22-23	21.118297457005674	30.38509141022614	28.593456340682256	19.90315479208593
24-25	20.680063311827606	30.45959810575261	28.547972652722674	20.31236592969711
26-27	20.370905965945912	30.4371460147752	28.614615715140335	20.57733230413855
28-29	20.32196103212728	30.511524355526966	28.754853235552087	20.41166137679367
30-31	20.602422546484366	30.92227085544313	28.342404549198903	20.1329020488736
32-33	20.203240747952922	30.615258928094867	28.83205331561322	20.34944700833899
34-35	20.49500046415551	30.29546211692965	28.73334578742801	20.476191631486827
36-37	20.71142522958995	30.502806237741186	28.36987655210188	20.415891980566983
38-39	20.49773150679054	30.525941297941543	28.62755554261548	20.348771652652438
40-41	20.383273036707646	30.246444899712486	28.81481504182456	20.55546702175531
42-43	20.301557525785306	30.22204032430044	28.805275437012597	20.671126712901653
44-45	20.56448291385569	30.350241214276817	28.649615110773386	20.435660761094102
46-47	20.595576022342975	30.568495213961626	28.50158351282281	20.33434525087259
48-49	20.47818717808701	30.26362704347546	28.601364504991487	20.656821273446045
50-51	20.46629825082287	30.227045043146795	28.588660837140406	20.717995868889933
52-53	20.46586976390073	30.306116754636538	28.561477153139847	20.666536328322884
54-55	20.636972168495394	30.56296152892322	28.401436079177948	20.398630223403433
56-57	20.604144288540304	30.289971994461208	28.27195661312261	20.83392710387588
58-59	20.476169374761437	30.095091613617704	28.64407708214596	20.7846619294749
60-61	20.68542087140074	30.39707162153275	28.245571580988525	20.671935926077982
62-63	20.541653211652765	30.347756725176666	28.398704590205924	20.711885472964646
64-65	20.573542616471673	30.174494268736545	28.54128082833382	20.710682286457953
66-67	20.418803575961604	30.227389622601514	28.38192232197445	20.971884479462428
68-69	20.81226518799453	30.531386519443004	28.125867087801858	20.5304812047606
70-71	20.793134303660022	30.185623754447217	28.220191259705224	20.801050682187537
72-73	20.601491509773158	30.33145541200889	28.511462623390205	20.555590454827744
74-75	20.631898299717555	30.442614829077947	28.343131196020245	20.582355675184253
76-77	20.702270000139187	30.43533502323325	28.132543112942756	20.72985186368481
78-79	20.78689289048789	30.339859256217427	28.375825636248113	20.497422217046566
80-81	20.711814093233045	30.296659652992318	28.334086546706743	20.65743970706789
82-83	20.58348894766825	30.260391319344365	28.591964719783736	20.564155013203646
84-85	20.579250597173793	30.210053207690052	28.361314248461333	20.84938194667482
86-87	20.830414884364295	30.269065139014796	28.233457358635228	20.667062617985682
88-89	20.652864054039004	30.139980803975284	28.362075438640407	20.84507970334531
90-91	20.565563105540203	30.091849988015458	28.31655433632902	21.026032570115316
92-93	20.697157852640633	30.24330399438997	28.25234010973885	20.80719804323055
94-95	20.548569470224713	30.182123143709035	28.372025972974633	20.897281413091626
96-97	20.54947008106986	30.312647316747544	28.281738315914524	20.856144286268076
98-99	20.57670075912797	30.257686191435813	28.22151156710181	20.944101482334414
100	20.67113648263796	30.370790391172754	28.01236223388631	20.945710892302976
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	21320.0
1	15572.0
2	8633.0
3	6280.0
4	4239.0
5	3017.0
6	2480.0
7	2385.0
8	2568.0
9	2544.5
10	2104.5
11	1650.5
12	1546.5
13	1602.0
14	1643.5
15	1765.0
16	2064.0
17	2527.0
18	3284.5
19	4303.0
20	5864.0
21	8331.5
22	12386.0
23	18980.0
24	25987.5
25	32882.5
26	42663.0
27	55409.0
28	72273.5
29	87372.0
30	95965.0
31	105786.0
32	116565.5
33	125824.0
34	134881.5
35	141006.0
36	143047.5
37	143493.5
38	142304.5
39	139942.0
40	137700.0
41	133223.0
42	125817.0
43	119823.5
44	116040.0
45	112200.5
46	109476.5
47	107127.0
48	104885.5
49	103241.5
50	101709.5
51	97286.5
52	90906.5
53	85466.0
54	81875.0
55	79805.0
56	73551.5
57	65301.5
58	59335.0
59	53407.5
60	46063.0
61	37767.5
62	30974.0
63	25155.0
64	19566.0
65	15565.5
66	12516.5
67	9630.5
68	7226.0
69	5429.5
70	4046.5
71	3136.5
72	2449.0
73	1913.0
74	1605.0
75	1283.5
76	959.0
77	763.5
78	574.5
79	431.5
80	325.5
81	226.0
82	160.5
83	124.0
84	112.5
85	101.0
86	85.0
87	77.5
88	75.5
89	64.5
90	56.0
91	47.0
92	36.0
93	33.5
94	27.5
95	21.5
96	24.5
97	22.0
98	19.0
99	23.5
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18505768890199956
2	0.17888229442953898
3	0.1930193545028578
4	0.183067272501785
5	0.19516287985693498
6	0.08053530973175821
7	0.08237261717811012
8	0.06991975559728059
9	0.0636422884889116
10-11	0.07781762580069602
12-13	0.0771796718262683
14-15	0.0749723510747483
16-17	0.07086392747943364
18-19	0.07557202781071039
20-21	0.07691173115700864
22-23	0.07506166463116819
24-25	0.07456406053111456
26-27	0.07899146111364308
28-29	0.07559754596968749
30-31	0.08133913173953718
32-33	0.07851937517256656
34-35	0.07820039818535268
36-37	0.08886698863778454
38-39	0.0840440565911108
40-41	0.08002494655221602
42-43	0.08352093433208005
44-45	0.09417476570502335
46-47	0.08355921157054572
48-49	0.08961977432760927
50-51	0.0858813640374627
52-53	0.08491167399633254
54-55	0.08663414972728743
56-57	0.0797187286444907
58-59	0.08799937123256281
60-61	0.08422268370395057
62-63	0.07531684622093929
64-65	0.0781366027879099
66-67	0.07849385701358945
68-69	0.07687345391854297
70-71	0.07262468044885421
72-73	0.07007286455114324
74-75	0.07548271425429048
76-77	0.08067565960613232
78-79	0.0768096585211002
80-81	0.08501374663224097
82-83	0.08631517274007357
84-85	0.08830558914028812
86-87	0.08455441977065299
88-89	0.08635344997853923
90-91	0.08465649240656144
92-93	0.07492131475679409
94-95	0.07498511015423685
96-97	0.0739005883977097
98-99	0.08417164738599635
100	0.092579880768954
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3918778.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.17666365120807
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.7490880999099	51.451752523758834
2	14.328947566930017	18.964838872250482
3	4.208375877387162	8.354888248671239
4	1.6080475892067532	4.256608977842853
5	0.7385433582273774	2.4437167704623413
6	0.3819877315013024	1.5167204175869804
7	0.23718777934214297	1.098740712699137
8	0.14752285549694485	0.7810056311269674
9	0.10896401015148438	0.6489787184893444
>10	0.4442640293745364	5.048101515171634
>50	0.02712164298085502	1.234507930424199
>100	0.01760009675468833	2.35737716016223
>500	0.001656108279068627	0.6902236228589035
>1k	6.54740321255419E-4	0.5813414359966594
>5k	0.0	0.0
>10k+	3.851413654443417E-5	0.5711974624981606
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22411	0.5718874608360055	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.55181589771097E-5	0.0
5	0.0	0.0	0.0	2.55181589771097E-5	0.0
6	0.0	0.0	0.0	2.55181589771097E-5	0.0
7	0.0	0.0	0.0	2.55181589771097E-5	0.0
8	0.0	0.0	0.0	2.55181589771097E-5	0.0
9	0.0	0.0	0.0	2.55181589771097E-5	0.0
10-11	0.0	0.0	0.0	2.55181589771097E-5	0.0
12-13	0.0	0.0	0.0	3.827723846566455E-5	0.0
14-15	0.0	0.0	0.0	5.10363179542194E-5	0.0
16-17	0.0	0.0	0.0	6.379539744277426E-5	0.0
18-19	0.0	0.0	0.0	1.020726359084388E-4	0.0
20-21	0.0	0.0	0.0	1.275907948855485E-4	0.0
22-23	0.0	0.0	0.0	2.296634307939873E-4	0.0
24-25	0.0	0.0	0.0	4.593268615879746E-4	0.0
26-27	0.0	0.0	0.0	9.696900411301685E-4	0.0
28-29	0.0	0.0	0.0	0.002373188784871202	0.0
30-31	0.0	0.0	0.0	0.006086080916040664	0.0
32-33	0.0	0.0	0.0	0.012988742919348838	0.0
34-35	0.0	0.0	0.0	0.02342566994098671	0.0
36-37	0.0	0.0	0.0	0.04020385946843633	0.0
38-39	0.0	0.0	0.0	0.06315744346834651	0.0
40-41	0.0	0.0	0.0	0.08973460604300626	0.0
42-43	0.0	0.0	0.0	0.11558450108681839	0.0
44-45	0.0	0.0	0.0	0.1480818765441676	0.0
46-47	0.0	0.0	0.0	0.18759674572022195	0.0
48-49	0.0	0.0	0.0	0.23105417045823978	0.0
50-51	0.0	0.0	0.0	0.2775865333529993	0.0
52-53	0.0	0.0	0.0	0.3213884532372081	0.0
54-55	0.0	0.0	0.0	0.37213131236319075	0.0
56-57	0.0	0.0	0.0	0.43500805608278903	0.0
58-59	0.0	0.0	0.0	0.5091638260702699	1.275907948855485E-5
60-61	0.0	0.0	0.0	0.588410468773684	2.55181589771097E-5
62-63	0.0	0.0	0.0	0.6700940956594121	2.55181589771097E-5
64-65	0.0	0.0	0.0	0.7563327139225544	2.55181589771097E-5
66-67	0.0	0.0	0.0	0.860191620959391	2.55181589771097E-5
68-69	0.0	0.0	0.0	0.9808159584441885	2.55181589771097E-5
70-71	0.0	0.0	0.0	1.1154114879689536	2.55181589771097E-5
72-73	0.0	0.0	0.0	1.2598697859383716	2.55181589771097E-5
74-75	0.0	0.0	0.0	1.4067140317721494	2.55181589771097E-5
76-77	0.0	0.0	0.0	1.5679377601895284	2.55181589771097E-5
78-79	0.0	0.0	0.0	1.7530082081710165	2.55181589771097E-5
80-81	0.0	0.0	0.0	1.9663400172196537	2.55181589771097E-5
82-83	0.0	0.0	0.0	2.1918695062593496	2.55181589771097E-5
84-85	0.0	0.0	0.0	2.4197211477659617	2.55181589771097E-5
86-87	0.0	0.0	0.0	2.661658813027939	2.55181589771097E-5
88	0.0	0.0	0.0	2.8634181369804566	2.55181589771097E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5640	0.0	44.802425	1
GTATCAA	10125	0.0	38.8059	1
TCAACGC	11240	0.0	33.945137	4
ATCAACG	11270	0.0	33.858242	3
TATCAAC	12080	0.0	32.289864	2
CAACGCA	12190	0.0	31.337894	5
AACGCAG	13290	0.0	28.784344	6
GTACATG	20965	0.0	28.437283	1
TACATGG	21010	0.0	27.545942	2
CATGGGT	10345	0.0	26.786	4
ACATGGG	21465	0.0	25.931122	3
CGCAGAG	14445	0.0	25.799248	8
ACGCAGA	15065	0.0	25.2054	7
ATGGGTG	5595	0.0	23.37557	5
GCAGAGT	16120	0.0	22.18559	9
ATGGGTA	4700	0.0	21.220495	5
TCTGTCG	775	0.0	20.617147	8
GTGGTAT	3135	0.0	19.962753	1
TGGTATC	2975	0.0	19.76977	2
CATGGGG	8485	0.0	19.29525	4
>>END_MODULE