FastQCFastQC Report
Wed 25 May 2016
SRR2431397_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431397_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3918778
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312880.7984121580758082No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249490.6366525483199099No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164000.4184978072245991No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53240.13585867839413204No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52100.13294960827074154No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48230.12307408074660009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116950.060.5950281
GTATCAA233400.046.299161
TATCAAC298850.035.843282
ATCAACG298250.035.8031963
TCAACGC300900.035.440554
CAACGCA310500.034.3448035
AACGCAG322550.033.074846
CGCAGAG361250.029.4145158
ACGCAGA362950.029.3803317
GTGGTAT43000.028.2146841
TGGTATC42800.027.6862452
CATGGGT100550.027.356054
GTACATG201950.027.0341221
GCAGAGT389700.027.0138599
ATAACGC4450.026.4158843
TACATGG202950.026.2048022
TATAACG4400.025.6487312
ACATGGG200900.025.6282713
GAGTACT263700.024.11360512-13
ATGGGTG56300.023.7186265
Produced by FastQC (version 0.11.5)