##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431397_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3918778
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.66063961775839	34.0	31.0	34.0	31.0	34.0
2	32.78614634460028	34.0	31.0	34.0	31.0	34.0
3	32.86603272754925	34.0	31.0	34.0	31.0	34.0
4	36.331701362006214	37.0	37.0	37.0	35.0	37.0
5	36.24088529638576	37.0	37.0	37.0	35.0	37.0
6	36.281887363867	37.0	37.0	37.0	35.0	37.0
7	36.238148218653876	37.0	37.0	37.0	35.0	37.0
8	36.254462232869535	37.0	37.0	37.0	35.0	37.0
9	38.05444248181448	39.0	39.0	39.0	35.0	39.0
10-11	38.03130019102894	39.0	39.0	39.0	35.0	39.0
12-13	38.01521596783488	39.0	39.0	39.0	35.0	39.0
14-15	39.48082552775381	41.0	39.0	41.0	36.0	41.0
16-17	39.512938599736955	41.0	39.0	41.0	36.5	41.0
18-19	39.58821767908262	41.0	40.0	41.0	37.0	41.0
20-21	39.58624946348071	41.0	40.0	41.0	37.0	41.0
22-23	39.522642262460394	41.0	40.0	41.0	36.0	41.0
24-25	39.472744437168934	41.0	39.0	41.0	36.0	41.0
26-27	39.35746819544256	41.0	39.0	41.0	36.0	41.0
28-29	39.27368314816506	41.0	39.0	41.0	36.0	41.0
30-31	39.090558459805585	41.0	39.0	41.0	35.0	41.0
32-33	38.859186077904894	41.0	38.0	41.0	35.0	41.0
34-35	38.72154508369701	41.0	38.0	41.0	35.0	41.0
36-37	38.57501573194501	41.0	38.0	41.0	35.0	41.0
38-39	38.424792116317896	41.0	38.0	41.0	35.0	41.0
40-41	38.2803931480681	41.0	38.0	41.0	34.5	41.0
42-43	38.10514400152293	40.0	38.0	41.0	34.0	41.0
44-45	37.84963399304579	40.0	38.0	41.0	33.5	41.0
46-47	37.47302819909676	40.0	38.0	41.0	33.0	41.0
48-49	37.12865094680025	40.0	38.0	41.0	32.0	41.0
50-51	36.96134904809612	40.0	37.0	41.0	31.0	41.0
52-53	36.90315935222664	40.0	37.0	41.0	31.0	41.0
54-55	36.7695910561915	40.0	37.0	41.0	31.0	41.0
56-57	36.62879882453152	40.0	36.0	41.0	31.0	41.0
58-59	36.46010108253134	40.0	36.0	41.0	31.0	41.0
60-61	36.316210308417574	40.0	35.5	41.0	31.0	41.0
62-63	36.165424527748186	39.0	35.0	41.0	31.0	41.0
64-65	35.93988853157796	39.0	35.0	41.0	30.0	41.0
66-67	35.68729103817567	39.0	35.0	41.0	30.0	41.0
68-69	35.40121397537702	38.5	35.0	41.0	29.0	41.0
70-71	35.10569646966478	37.0	35.0	40.5	29.0	41.0
72-73	34.79062873681541	37.0	35.0	40.0	29.0	41.0
74-75	34.45612152563886	36.5	35.0	39.0	29.0	41.0
76-77	34.11654998063172	36.0	35.0	39.0	29.0	41.0
78-79	33.787393799801876	36.0	35.0	38.5	28.5	40.0
80-81	33.511438004398315	35.0	34.5	37.0	28.5	39.0
82-83	33.29613823492936	35.0	35.0	37.0	29.0	39.0
84-85	33.02040904077751	35.0	34.0	36.5	29.0	39.0
86-87	32.76524709998883	35.0	34.0	36.0	29.0	37.0
88-89	32.52418853530361	35.0	34.0	36.0	28.0	37.0
90-91	32.33259092502816	35.0	34.0	36.0	27.5	37.0
92-93	32.16743561385718	35.0	34.0	35.0	27.0	36.0
94-95	32.025929894472206	35.0	34.0	35.0	27.0	36.0
96-97	31.883157708857198	35.0	34.0	35.0	26.5	36.0
98-99	31.76587752610635	35.0	34.0	35.0	26.0	36.0
100	31.664115446192664	35.0	34.0	35.0	25.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.03600838351284352
1101	2	-6.038383356710142E-4
1101	3	-0.010632940992636009
1101	4	0.052448924420936294
1101	5	-0.02558353944666436
1101	6	0.007480761964806959
1101	7	-0.006709624495755406
1101	8	0.01410286790767401
1101	9	0.004242273976949207
1101	10-11	0.0289111789573937
1101	12-13	0.02158952922822266
1101	14-15	0.030527701264801976
1101	16-17	-0.027708432824525175
1101	18-19	-0.056979966581842234
1101	20-21	-0.03661049802762051
1101	22-23	0.012142022592485091
1101	24-25	0.002414832102346054
1101	26-27	0.01967752013329971
1101	28-29	0.03893439887748684
1101	30-31	0.02870695413874813
1101	32-33	0.020380026289814168
1101	34-35	0.035284235721057655
1101	36-37	0.09686083283052227
1101	38-39	-0.003047145375887794
1101	40-41	0.0033201908950317716
1101	42-43	-0.007085476630443566
1101	44-45	0.013337533352782316
1101	46-47	0.06977085648174608
1101	48-49	0.10675830093973815
1101	50-51	0.08725577256183925
1101	52-53	0.16492869908763197
1101	54-55	0.16210215032783282
1101	56-57	0.14197844125411763
1101	58-59	0.11142813394338447
1101	60-61	0.15488505066328884
1101	62-63	0.16220678889886386
1101	64-65	0.13669065749650855
1101	66-67	0.07343387888725061
1101	68-69	0.08993773937622507
1101	70-71	0.15306066557944575
1101	72-73	0.1054911420856044
1101	74-75	0.058800963060015476
1101	76-77	0.06377910753982263
1101	78-79	0.09905000135768205
1101	80-81	0.10971003784004552
1101	82-83	0.13353610454525722
1101	84-85	0.15205067420781404
1101	86-87	0.22081258480177723
1101	88-89	0.21855731062925088
1101	90-91	0.17123375837616805
1101	92-93	0.2014302790819542
1101	94-95	0.2245752312436835
1101	96-97	0.20357674793704206
1101	98-99	0.20863009995420967
1101	100	0.20827532779609115
1102	1	-0.06848517807191712
1102	2	-0.03271231773862837
1102	3	-0.024227418261901335
1102	4	0.008123254331799501
1102	5	-0.026518493304479307
1102	6	-0.03936772543951861
1102	7	-0.0727613482218814
1102	8	-0.04073945476613261
1102	9	-0.03501194863207502
1102	10-11	-0.01917315305333034
1102	12-13	-0.07960760232351305
1102	14-15	-0.0654300282344451
1102	16-17	-0.07278116721514039
1102	18-19	-0.070696370691401
1102	20-21	-0.012504390794454423
1102	22-23	-0.02116496608418572
1102	24-25	-0.06060478367307098
1102	26-27	-0.0828508858193544
1102	28-29	-0.06312788298838257
1102	30-31	-0.04087279525144538
1102	32-33	-0.03332805488619073
1102	34-35	0.0030934817469940867
1102	36-37	-0.04385583388590675
1102	38-39	-0.08615559591821409
1102	40-41	-0.1179842798159072
1102	42-43	-0.04292332192552095
1102	44-45	-0.06768811566311683
1102	46-47	-0.1187241822263374
1102	48-49	-0.12843669243724776
1102	50-51	-0.09309576299794031
1102	52-53	-0.09624398862839456
1102	54-55	-0.12533786434133276
1102	56-57	-0.14114454534360021
1102	58-59	-0.14240221306092593
1102	60-61	-0.14976423543414796
1102	62-63	-0.1675794079316404
1102	64-65	-0.19367065273637962
1102	66-67	-0.13229862323500896
1102	68-69	-0.1342586008717177
1102	70-71	-0.1671679037841045
1102	72-73	-0.11758299229447289
1102	74-75	-0.11880536037909906
1102	76-77	-0.15520393895365459
1102	78-79	-0.17692500284702106
1102	80-81	-0.17303765169219787
1102	82-83	-0.19167083054021106
1102	84-85	-0.122818280484978
1102	86-87	-0.12122544791323975
1102	88-89	-0.1434575353047336
1102	90-91	-0.1306857566607249
1102	92-93	-0.10768755340496483
1102	94-95	-0.10520199328904312
1102	96-97	-0.07688106192414068
1102	98-99	-0.062025529013503444
1102	100	-0.10700894569720987
1103	1	-0.12322985791301733
1103	2	-0.09811360170689909
1103	3	-0.08808156298683656
1103	4	-0.03816517521880769
1103	5	-0.09656011336814885
1103	6	-0.08600920052706584
1103	7	-0.12293293873652544
1103	8	-0.07942782508024493
1103	9	-0.07239679472102978
1103	10-11	-0.0332804806271767
1103	12-13	-0.09225339430750523
1103	14-15	-0.06652941710471083
1103	16-17	-0.10117096991015728
1103	18-19	-0.06596985922137577
1103	20-21	-0.11205020624063877
1103	22-23	-0.0834237152102304
1103	24-25	-0.11718484292934761
1103	26-27	-0.1457757364425234
1103	28-29	-0.04864712879114563
1103	30-31	-0.06518027714673735
1103	32-33	-0.15322144156203166
1103	34-35	-0.08282933120018043
1103	36-37	-0.06837377097259889
1103	38-39	-0.09294810675433496
1103	40-41	-0.13697432234949503
1103	42-43	-0.139994496449674
1103	44-45	-0.20802731689511234
1103	46-47	-0.16583360335032182
1103	48-49	-0.21582312698389217
1103	50-51	-0.25098226071204977
1103	52-53	-0.15381592227462448
1103	54-55	-0.10729465384213199
1103	56-57	-0.10043212763436316
1103	58-59	-0.12552775511439052
1103	60-61	-0.10802803511393932
1103	62-63	-0.2218871846496242
1103	64-65	-0.2351469517115632
1103	66-67	-0.22683702670276062
1103	68-69	-0.19371990295211106
1103	70-71	-0.18764156270552235
1103	72-73	-0.22951123714885568
1103	74-75	-0.19808764760487918
1103	76-77	-0.20683825485622975
1103	78-79	-0.1699867803769024
1103	80-81	-0.14807066627090393
1103	82-83	-0.16268446000161418
1103	84-85	-0.21184436493801684
1103	86-87	-0.1739291914073675
1103	88-89	-0.16984464516222175
1103	90-91	-0.19578886487066427
1103	92-93	-0.2149700694428276
1103	94-95	-0.2573432651528975
1103	96-97	-0.22942397562280092
1103	98-99	-0.21061258045035913
1103	100	-0.22128849422416152
1104	1	-0.08384608834808205
1104	2	-0.06992760950900845
1104	3	-0.07119020124163455
1104	4	-0.028729052965424273
1104	5	-0.039035531351181874
1104	6	-0.06805658601397369
1104	7	-0.08932084804409612
1104	8	-0.14151900713466858
1104	9	-0.08180607964230546
1104	10-11	-0.1052061525397221
1104	12-13	-0.0871616302165279
1104	14-15	-0.08756405592991001
1104	16-17	-0.05105358335832477
1104	18-19	-0.03941464041601961
1104	20-21	-0.0022182256883738205
1104	22-23	-0.05396900879289035
1104	24-25	-0.01673452963333233
1104	26-27	-0.08142524578142485
1104	28-29	-0.0802900111144993
1104	30-31	-0.07586153532493256
1104	32-33	-0.04856550105583324
1104	34-35	-0.1079833474972034
1104	36-37	-0.12587550912591894
1104	38-39	-0.1807471272682193
1104	40-41	-0.24333195473985825
1104	42-43	-0.22716510415458657
1104	44-45	-0.19385102408237742
1104	46-47	-0.14669843255227732
1104	48-49	-0.09192094829438702
1104	50-51	-0.17185561543364258
1104	52-53	-0.10795147252867565
1104	54-55	-0.07452250738190713
1104	56-57	-0.17982535605830918
1104	58-59	-0.08160725237190292
1104	60-61	-0.12330291426943774
1104	62-63	-0.16306099202551394
1104	64-65	-0.13739274984830985
1104	66-67	-0.21007792373676892
1104	68-69	-0.24281552770194992
1104	70-71	-0.20684642794356023
1104	72-73	-0.2301846032541377
1104	74-75	-0.18938522190175178
1104	76-77	-0.2342472649779168
1104	78-79	-0.21618313172379544
1104	80-81	-0.21109731448703428
1104	82-83	-0.21004512926408836
1104	84-85	-0.22611119166983684
1104	86-87	-0.20916930137616419
1104	88-89	-0.17594995460859053
1104	90-91	-0.1382525013567104
1104	92-93	-0.18185688689633395
1104	94-95	-0.18012618632764088
1104	96-97	-0.1352806954826704
1104	98-99	-0.1486020174967706
1104	100	-0.09874302376603339
1105	1	-0.18685928014243558
1105	2	-0.16234232775475022
1105	3	-0.12312877513311804
1105	4	-0.06382799142308926
1105	5	-0.14687772378478314
1105	6	-0.07922239389031205
1105	7	-0.06798999745424084
1105	8	-0.09872388674259724
1105	9	-0.04389783138087466
1105	10-11	-0.05426967558177154
1105	12-13	-0.0922319234966551
1105	14-15	-0.12121487533212161
1105	16-17	-0.12721960916077535
1105	18-19	-0.21311967499363504
1105	20-21	-0.11747777569514994
1105	22-23	-0.18317643571499787
1105	24-25	-0.16372533315114168
1105	26-27	-0.11110411953924881
1105	28-29	-0.1209247572547838
1105	30-31	-0.11475977351333455
1105	32-33	-0.07900263663493945
1105	34-35	-0.1357948365621624
1105	36-37	-0.08424798844715298
1105	38-39	-0.028698379616983516
1105	40-41	-0.020421503003227315
1105	42-43	-0.09888350002464819
1105	44-45	-0.015368806853629735
1105	46-47	-0.015542988273558933
1105	48-49	-0.01144567564460175
1105	50-51	-0.02401901643707305
1105	52-53	0.043570136056651165
1105	54-55	0.07650210858384554
1105	56-57	0.10218847498598649
1105	58-59	0.12162035450874953
1105	60-61	0.06798464166492835
1105	62-63	0.02220429670237678
1105	64-65	-0.029228268899892385
1105	66-67	-4.2452312076335375E-4
1105	68-69	0.053433358453446544
1105	70-71	0.0842305871466209
1105	72-73	0.1181056452784901
1105	74-75	0.06256001084334173
1105	76-77	0.01658351781269829
1105	78-79	0.11564546212004245
1105	80-81	0.12433032919891929
1105	82-83	0.07881012905100704
1105	84-85	0.05339866827630857
1105	86-87	0.02228603345520952
1105	88-89	0.11542174502141478
1105	90-91	0.09307401492479528
1105	92-93	0.014629417657836541
1105	94-95	0.051544411080492125
1105	96-97	0.08177686606935097
1105	98-99	0.08376134282711334
1105	100	0.09045679995594824
1106	1	-0.176882307808512
1106	2	-0.1234147463125197
1106	3	-0.12558970136849723
1106	4	-0.07996373723673145
1106	5	-0.06881432326120063
1106	6	-0.10239594960054177
1106	7	-0.08877331148435275
1106	8	-0.06343908434467949
1106	9	-0.1227989965253542
1106	10-11	-0.11250215426603205
1106	12-13	-0.12115896130185178
1106	14-15	-0.16020172260369492
1106	16-17	-0.18514087619434605
1106	18-19	-0.1159448497793889
1106	20-21	-0.12657808929035497
1106	22-23	-0.16180451425047693
1106	24-25	-0.1026999926206642
1106	26-27	-0.12863459374103314
1106	28-29	-0.14004610361473624
1106	30-31	-0.24262317314455828
1106	32-33	-0.13917512456220038
1106	34-35	-0.1442540283253635
1106	36-37	-0.12968606361042845
1106	38-39	-0.10929562183909525
1106	40-41	-0.2743731160200795
1106	42-43	-0.28662863570611563
1106	44-45	-0.28476390909496274
1106	46-47	-0.29572417791576555
1106	48-49	-0.318478131362248
1106	50-51	-0.3572697181163136
1106	52-53	-0.31501437402189225
1106	54-55	-0.29109823413057256
1106	56-57	-0.3050635842594076
1106	58-59	-0.2833978320872035
1106	60-61	-0.3202640745062837
1106	62-63	-0.3580622310374295
1106	64-65	-0.31188007161436815
1106	66-67	-0.3349128209521268
1106	68-69	-0.418977620102595
1106	70-71	-0.3894675665540177
1106	72-73	-0.4243728370278248
1106	74-75	-0.4243530431615383
1106	76-77	-0.37977131959403465
1106	78-79	-0.3906785159597632
1106	80-81	-0.33438804122383914
1106	82-83	-0.3392469803123248
1106	84-85	-0.3031745022148371
1106	86-87	-0.3262952324135924
1106	88-89	-0.38390361082013413
1106	90-91	-0.4263033281155728
1106	92-93	-0.3640431263943782
1106	94-95	-0.3587429451198858
1106	96-97	-0.40693162847643194
1106	98-99	-0.43143615944840974
1106	100	-0.3893692761166001
1107	1	-0.24794217744417324
1107	2	-0.18986326338961845
1107	3	-0.17183924638609227
1107	4	-0.1348447262829282
1107	5	-0.1940304939149513
1107	6	-0.16253001947823265
1107	7	-0.1620528730696975
1107	8	-0.10163427413067438
1107	9	-0.11598817345743129
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2216	34-35	0.06416643501928831
2216	36-37	0.07086680004586299
2216	38-39	0.08133726224917126
2216	40-41	0.023069802881927615
2216	42-43	0.048307712873238984
2216	44-45	0.0381890796784603
2216	46-47	0.061368183483303085
2216	48-49	0.059032329739288514
2216	50-51	0.15902728868621097
2216	52-53	0.10039698437408617
2216	54-55	0.1076931612935681
2216	56-57	0.10641856574626019
2216	58-59	0.13054124792892452
2216	60-61	0.10537778443947587
2216	62-63	0.07389110735606863
2216	64-65	0.11093617792349875
2216	66-67	0.1150845724412477
2216	68-69	0.08970257126537717
2216	70-71	0.09529277629367527
2216	72-73	0.13872918746854168
2216	74-75	0.10596502023974352
2216	76-77	0.11787615814338892
2216	78-79	0.15636241159043607
2216	80-81	0.1661422477842649
2216	82-83	0.1419504421602582
2216	84-85	0.14939269442995595
2216	86-87	0.12750921756610722
2216	88-89	0.15187400211169688
2216	90-91	0.08700813363594051
2216	92-93	0.10486663288430265
2216	94-95	0.10066979836467027
2216	96-97	0.1069934619536852
2216	98-99	0.04630820274842762
2216	100	0.07828928806881663
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	55.0
3	341.0
4	98.0
5	155.0
6	314.0
7	706.0
8	1409.0
9	2428.0
10	3431.0
11	5160.0
12	7805.0
13	11678.0
14	13519.0
15	17839.0
16	22821.0
17	34343.0
18	40904.0
19	20982.0
20	12289.0
21	10867.0
22	10420.0
23	10343.0
24	11211.0
25	13044.0
26	16043.0
27	20505.0
28	25285.0
29	31641.0
30	40376.0
31	51111.0
32	64411.0
33	84200.0
34	116967.0
35	192256.0
36	306082.0
37	635468.0
38	1225807.0
39	847618.0
40	8846.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.75685473097208	21.045707733763148	14.863147815874717	22.334289719390053
2	14.17114339647583	22.113118547275974	41.040479538977856	22.67525851727034
3	16.240266963292544	28.654228488180262	32.410128661220526	22.695375887306664
4	10.723623537745695	18.90010100087323	40.53840253262624	29.837872928754834
5	10.876192012875476	39.24400495901865	35.86038871516892	14.019414312936954
6	26.39919893395339	40.787561836878744	18.890455136779885	13.922784092387985
7	23.370499681278194	35.66106577101331	23.96655283866552	17.00188170904297
8	24.413707538421416	35.17254613555552	21.64103197476356	18.772714351259502
9	25.604882749103293	15.849744920352682	22.644468578964453	35.90090375157958
10-11	22.169883060484672	27.72821017164024	30.132390760589146	19.969516007285947
12-13	24.17821320830116	26.14350698100275	30.15670956609433	19.52157024460176
14-15	21.40694660604571	27.193382016889455	29.146006521932737	22.2536648551321
16-17	18.74488679889496	30.568138843282266	29.654193220437598	21.032781137385175
18-19	18.640737455509022	27.96355593027612	34.0381757346434	19.357530879571456
20-21	20.472721343235058	26.976560039889986	33.730030126738484	18.820688490136465
22-23	19.881919777767372	27.071038626469257	33.99117045843262	19.055871137330747
24-25	19.5496937501555	27.1608301313356	33.87135401282671	19.418122105682194
26-27	19.248233981431685	27.2195183568324	33.94936807380372	19.58287958793219
28-29	19.220557046810317	27.209958321137762	34.08986690476841	19.479617727283514
30-31	19.559769524356586	27.606602130186154	33.63859103077232	19.195037314684942
32-33	19.183020195849366	27.387306486853124	34.025063247596286	19.404610069701224
34-35	19.467390096384285	27.27289546220272	33.705485219039126	19.554229222373873
36-37	19.71610466954498	27.46394051342615	33.23672472709929	19.583230089929575
38-39	19.607871157483757	27.52922337870296	33.302906867326534	19.559998596486754
40-41	19.546804053365236	27.37187715987816	33.326579510884606	19.754739275871998
42-43	19.548331211699427	27.422576523956003	33.05214294381394	19.976949320530633
44-45	19.924402347929103	27.61702803620266	32.605350272311036	19.8532193435572
46-47	20.048201342742576	28.024325486782143	31.94947241743297	19.978000753042313
48-49	20.121104217939617	27.99847962634227	31.486535264158544	20.393880891559565
50-51	20.106354840812873	28.113092161478793	31.19797711959765	20.58257587811069
52-53	20.153150896733766	28.304590533281086	31.099295834040934	20.44296273594421
54-55	20.300813181364592	28.547727467304902	30.922467642378827	20.22899170895168
56-57	20.194580331692478	28.201860825939278	30.89247937448484	20.7110794678834
58-59	20.1048190852598	27.935998501563763	31.306931390401843	20.6522510227746
60-61	20.23227170949156	28.183305328933777	31.008672581253695	20.57575038032097
62-63	20.097398753067317	28.080483094548853	31.191269327660752	20.630848824723074
64-65	20.075772316603395	27.903749534126444	31.36752661300705	20.65295153626311
66-67	20.063479271948175	27.994318099898706	31.134202035108306	20.808000593044813
68-69	20.484621882931787	28.211441023597605	30.786434966206837	20.51750212726377
70-71	20.374066734272983	27.993404046069603	30.784027420876882	20.848501798780532
72-73	20.26768586336665	28.13041583812278	30.975707316513013	20.626190981997553
74-75	20.329321555948848	28.268989105847055	30.705720581812557	20.69596875639154
76-77	20.410508871970777	28.227065494931285	30.542870091605835	20.8195555414921
78-79	20.502152536159812	28.196852082532963	30.720016678790007	20.580978702517218
80-81	20.346746481396323	28.173875697864265	30.72173416877151	20.757643651967904
82-83	20.197732579993453	28.170296119122355	30.97214988438848	20.659821416495713
84-85	20.166755279412126	28.20138582933555	30.65222680830926	20.979632082943063
86-87	20.30765505050518	28.29543009977526	30.50097902190697	20.895935827812593
88-89	20.08708172859464	28.092246663940447	30.655192718563974	21.16547888890094
90-91	19.964138928541715	28.129671470428967	30.591397629948858	21.314791971080457
92-93	20.07049971942505	28.213654953800187	30.568289952456645	21.147555374318117
94-95	19.888005217985103	28.088762397435513	30.702947926815515	21.320284457763872
96-97	19.84182546958263	28.240488571357485	30.546950607620115	21.37073535143977
98-99	19.811088683438935	28.130153083748215	30.53253954684761	21.52621868596524
100	19.882386565172236	28.217195701712527	30.362179969735404	21.538237763379836
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2772.0
1	1639.5
2	2460.0
3	4239.5
4	2537.5
5	1392.0
6	3452.0
7	9176.5
8	16397.5
9	17870.5
10	11273.0
11	5299.0
12	4293.5
13	4693.0
14	5208.0
15	5702.5
16	6377.0
17	7070.5
18	7900.5
19	8880.0
20	10337.0
21	12598.5
22	16267.5
23	22402.0
24	29073.0
25	35414.5
26	44495.5
27	56420.0
28	72338.0
29	87225.0
30	95612.0
31	104804.5
32	115075.5
33	123627.0
34	131774.0
35	138153.0
36	140591.5
37	141199.0
38	140191.5
39	137463.0
40	136016.5
41	131628.0
42	124738.0
43	120158.5
44	116294.0
45	112237.5
46	108913.0
47	106404.5
48	104561.0
49	102266.0
50	100313.0
51	95876.5
52	88809.0
53	82839.5
54	79183.5
55	76709.5
56	70158.5
57	62417.0
58	56705.5
59	51452.5
60	44480.5
61	35965.5
62	29479.0
63	23777.5
64	18009.5
65	14007.0
66	11179.5
67	8510.0
68	6283.5
69	4472.5
70	3179.5
71	2350.5
72	1799.0
73	1410.5
74	1189.5
75	914.0
76	562.5
77	432.0
78	358.0
79	224.5
80	98.0
81	38.5
82	20.5
83	17.5
84	10.0
85	3.5
86	2.5
87	2.0
88	2.5
89	2.5
90	0.5
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005282258908261708
2	0.004618786774856856
3	9.44171882153059E-4
4	0.0
5	0.04782102992310358
6	0.0
7	0.0
8	0.0
9	5.10363179542194E-5
10-11	0.0
12-13	0.0
14-15	2.55181589771097E-5
16-17	0.0
18-19	1.531089538626582E-4
20-21	0.0
22-23	3.827723846566455E-5
24-25	1.275907948855485E-5
26-27	1.4034987437410335E-4
28-29	1.275907948855485E-4
30-31	3.1897698721387126E-4
32-33	0.0013269442668097044
34-35	9.569309616416138E-4
36-37	0.0011483171539699367
38-39	0.001033485438572943
40-41	2.4242251028254216E-4
42-43	5.486404180078586E-4
44-45	1.4034987437410335E-4
46-47	1.9138619232832274E-4
48-49	1.1483171539699365E-4
50-51	2.4242251028254216E-4
52-53	7.272675308476265E-4
54-55	8.54858325733175E-4
56-57	0.0022455979899856536
58-59	6.634721334048522E-4
60-61	0.0012376307103898205
62-63	0.0010717626770386074
64-65	0.0016459212540235757
66-67	4.720859410765295E-4
68-69	2.9345882823676153E-4
70-71	3.062179077253164E-4
72-73	1.4034987437410335E-4
74-75	6.251948949391877E-4
76-77	8.165810872675104E-4
78-79	7.272675308476265E-4
80-81	9.186537231759492E-4
82-83	0.001301426107832595
84-85	0.0010590035975500526
86-87	0.0018117892873747887
88-89	0.0011610762334584915
90-91	0.0011227989949928268
92-93	9.952082001072784E-4
94-95	6.124358154506328E-4
96-97	1.4034987437410335E-4
98-99	2.041452718168776E-4
100	2.041452718168776E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3918778.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.76611239774799
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.3799479174032	46.55926335602716
2	15.787060027408309	19.502106481657922
3	4.62913477759345	8.577709769314835
4	1.777781570968548	4.392266253243533
5	0.8482354219683791	2.6196102206525054
6	0.4386119985848132	1.6254814802154274
7	0.2724665280380547	1.1780438736795849
8	0.1720350229637191	0.8500747651780983
9	0.11964129055663358	0.665079965993939
>10	0.5136554018263302	5.518545612705808
>50	0.034558377681582146	1.482206540271294
>100	0.02339471781135548	2.9231925627250126
>500	0.0021937881809952985	0.8826022522282486
>1k	0.0010761978831697137	1.0974363895574308
>5k	8.27844525515149E-5	0.2693161311030366
>10k+	1.2417667882727235E-4	1.8570643454462947
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31288	0.7984121580758082	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24949	0.6366525483199099	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16400	0.4184978072245991	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5324	0.13585867839413204	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5210	0.13294960827074154	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4823	0.12307408074660009	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.55181589771097E-5	0.0
5	0.0	0.0	0.0	2.55181589771097E-5	0.0
6	0.0	0.0	0.0	2.55181589771097E-5	0.0
7	0.0	0.0	0.0	2.55181589771097E-5	0.0
8	0.0	0.0	0.0	7.65544769313291E-5	0.0
9	0.0	0.0	0.0	7.65544769313291E-5	0.0
10-11	0.0	0.0	0.0	8.931355641988395E-5	0.0
12-13	0.0	0.0	0.0	1.531089538626582E-4	0.0
14-15	0.0	0.0	0.0	1.786271128397679E-4	0.0
16-17	0.0	0.0	0.0	1.9138619232832274E-4	0.0
18-19	0.0	0.0	0.0	2.296634307939873E-4	0.0
20-21	0.0	0.0	0.0	2.55181589771097E-4	0.0
22-23	0.0	0.0	0.0	3.572542256795358E-4	0.0
24-25	0.0	0.0	0.0	5.869176564735231E-4	0.0
26-27	0.0	0.0	0.0	0.0011483171539699367	0.0
28-29	0.0	0.0	0.0	0.002653888533619409	0.0
30-31	0.0	0.0	0.0	0.006302985267346097	0.0
32-33	0.0	0.0	0.0	0.013180129111677161	0.0
34-35	0.0	0.0	0.0	0.023770165087177685	0.0
36-37	0.0	0.0	0.0	0.040688704489001415	0.0
38-39	0.0	0.0	0.0	0.0639485063966369	0.0
40-41	0.0	0.0	0.0	0.09064050068669366	0.0
42-43	0.0	0.0	0.0	0.116656263763857	0.0
44-45	0.0	0.0	0.0	0.1491536392212062	0.0
46-47	0.0	0.0	0.0	0.18908955802038288	0.0
48-49	0.0	0.0	0.0	0.23278940526868325	0.0
50-51	0.0	0.0	0.0	0.27971729962758796	0.0
52-53	0.0	0.0	0.0	0.324144414406736	0.0
54-55	0.0	0.0	0.0	0.3755890229045891	0.0
56-57	0.0	0.0	0.0	0.43967787917560014	0.0
58-59	0.0	0.0	0.0	0.5148798936811424	0.0
60-61	0.0	0.0	0.0	0.5953131307769922	0.0
62-63	0.0	0.0	0.0	0.6786426789167439	0.0
64-65	0.0	0.0	0.0	0.7666165319903296	0.0
66-67	0.0	0.0	0.0	0.8720320467247697	0.0
68-69	0.0	0.0	0.0	0.9944171371789879	0.0
70-71	0.0	0.0	0.0	1.1311817102168074	0.0
72-73	0.0	0.0	0.0	1.2775028337915546	0.0
74-75	0.0	0.0	0.0	1.4262226643101497	0.0
76-77	0.0	0.0	0.0	1.589934413227797	0.0
78-79	0.0	0.0	0.0	1.7781818720019351	0.0
80-81	0.0	0.0	0.0	1.9951117414663448	0.0
82-83	0.0	0.0	0.0	2.223933073014088	0.0
84-85	0.0	0.0	0.0	2.4551020751877246	0.0
86-87	0.0	0.0	0.0	2.701696804463024	0.0
88	0.0	0.0	0.0	2.907692142805742	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11695	0.0	60.595028	1
GTATCAA	23340	0.0	46.29916	1
TATCAAC	29885	0.0	35.84328	2
ATCAACG	29825	0.0	35.803196	3
TCAACGC	30090	0.0	35.44055	4
CAACGCA	31050	0.0	34.344803	5
AACGCAG	32255	0.0	33.07484	6
CGCAGAG	36125	0.0	29.414515	8
ACGCAGA	36295	0.0	29.380331	7
GTGGTAT	4300	0.0	28.214684	1
TGGTATC	4280	0.0	27.686245	2
CATGGGT	10055	0.0	27.35605	4
GTACATG	20195	0.0	27.034122	1
GCAGAGT	38970	0.0	27.013859	9
ATAACGC	445	0.0	26.415884	3
TACATGG	20295	0.0	26.204802	2
TATAACG	440	0.0	25.648731	2
ACATGGG	20090	0.0	25.628271	3
GAGTACT	26370	0.0	24.113605	12-13
ATGGGTG	5630	0.0	23.718626	5
>>END_MODULE