FastQCFastQC Report
Wed 25 May 2016
SRR2431396_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431396_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2504966
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA364381.4546305219312357No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28660.11441273055203145No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26690.10654835235288623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37550.052.3669131
GTATCAA70800.041.128991
ATCAACG84300.033.089613
TCAACGC85500.032.5151634
CAACGCA91000.030.6016545
TATCAAC94250.030.5452272
AACGCAG98700.028.3283796
GTACATG162900.027.809671
CATGGGT70900.027.2684334
TACATGG162200.026.7685052
CGCAGAG108350.025.502288
ACATGGG164650.025.4554373
ACGCAGA111700.024.947797
ATGGGTG38150.024.5370925
GCAGAGT118400.022.4648769
GTGGTAT18450.021.6727071
TGGTATC17150.021.120292
ATGGGTA31200.020.956795
TACACGC3453.6379788E-1220.4520455
TATAACG1405.1825953E-420.1602752
Produced by FastQC (version 0.11.5)