##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431396_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2504966
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.13905178752925	31.0	31.0	34.0	26.0	34.0
2	30.29731820711339	33.0	31.0	34.0	26.0	34.0
3	30.437938878212318	33.0	31.0	34.0	26.0	34.0
4	33.53568711112246	37.0	35.0	37.0	26.0	37.0
5	33.75509448032428	37.0	35.0	37.0	28.0	37.0
6	33.73611218675224	37.0	35.0	37.0	28.0	37.0
7	33.65225635797053	37.0	35.0	37.0	28.0	37.0
8	33.59125912287831	37.0	35.0	37.0	27.0	37.0
9	34.935090536158974	39.0	37.0	39.0	25.0	39.0
10-11	34.97639967967629	39.0	37.0	39.0	26.5	39.0
12-13	34.90129925915162	39.0	37.0	39.0	26.0	39.0
14-15	36.18932153170941	40.0	38.0	41.0	26.0	41.0
16-17	36.215460808649695	40.0	38.0	41.0	27.0	41.0
18-19	36.22028342899664	40.0	37.0	41.0	27.0	41.0
20-21	36.29708846347615	40.0	37.0	41.0	27.0	41.0
22-23	36.28351143288971	40.0	37.0	41.0	27.0	41.0
24-25	36.35127303125073	40.0	37.0	41.0	27.0	41.0
26-27	36.40030443526978	40.0	37.0	41.0	28.0	41.0
28-29	36.37482045664492	40.0	37.0	41.0	28.0	41.0
30-31	36.30132005783711	40.0	37.0	41.0	28.0	41.0
32-33	36.24447158165021	40.0	36.5	41.0	28.0	41.0
34-35	36.16455612571188	40.0	36.0	41.0	27.5	41.0
36-37	36.02426460079698	40.0	36.0	41.0	27.0	41.0
38-39	35.88789608322029	40.0	36.0	41.0	27.0	41.0
40-41	35.81829553774382	40.0	36.0	41.0	26.5	41.0
42-43	35.64250153495097	40.0	35.0	41.0	26.0	41.0
44-45	35.48543213760187	40.0	35.0	41.0	25.5	41.0
46-47	35.34623643594364	40.0	35.0	41.0	25.0	41.0
48-49	35.20344946797681	40.0	35.0	41.0	25.0	41.0
50-51	35.058105778681224	39.5	35.0	41.0	25.0	41.0
52-53	34.89194543957882	39.0	34.5	41.0	25.0	41.0
54-55	34.721073060472676	39.0	34.0	41.0	24.5	41.0
56-57	34.52696962753187	39.0	34.0	41.0	24.0	41.0
58-59	34.29872621025595	38.5	34.0	41.0	23.5	41.0
60-61	34.05325820789584	38.0	33.0	40.0	23.0	41.0
62-63	33.945030591233575	38.0	33.0	40.0	22.5	41.0
64-65	33.694416211637204	37.5	33.0	40.0	21.5	41.0
66-67	33.42972758911698	37.0	33.0	40.0	20.5	41.0
68-69	33.1406829473933	36.5	33.0	40.0	20.0	41.0
70-71	32.818662009783765	36.0	32.5	39.0	18.5	41.0
72-73	32.482266026764435	35.5	32.0	39.0	17.0	41.0
74-75	32.20439498979228	35.0	32.0	39.0	13.5	40.5
76-77	31.947105868902014	35.0	32.0	37.5	11.5	40.0
78-79	31.610766772882346	35.0	32.0	37.0	7.5	39.0
80-81	31.295284446974527	35.0	31.5	37.0	4.5	39.0
82-83	30.977954790603945	35.0	31.0	36.0	2.0	39.0
84-85	30.687968020324426	35.0	31.0	36.0	2.0	37.0
86-87	30.41646752890059	35.0	31.0	35.5	2.0	37.0
88-89	30.159385197244195	35.0	31.0	35.0	2.0	37.0
90-91	29.935437047848154	35.0	31.0	35.0	2.0	36.0
92-93	29.709924605763113	35.0	30.5	35.0	2.0	36.0
94-95	29.49815107270917	35.0	30.0	35.0	2.0	36.0
96-97	29.30053421882772	34.0	30.0	35.0	2.0	35.5
98-99	29.08634907619505	34.0	30.0	35.0	2.0	35.0
100	28.914819203134893	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3516679690926807
1101	2	0.3328313793568505
1101	3	0.33359874801270806
1101	4	0.4729311121198947
1101	5	0.41641197543039254
1101	6	0.5250897091151998
1101	7	0.3686285235670752
1101	8	0.3592954733163012
1101	9	0.38933655862575733
1101	10-11	0.2745128632475371
1101	12-13	0.26564960733942655
1101	14-15	0.3075560874194565
1101	16-17	0.2563900455608277
1101	18-19	0.20006736669592584
1101	20-21	0.19666941887548717
1101	22-23	0.3069637369864182
1101	24-25	0.29616793309882894
1101	26-27	0.20320566708792143
1101	28-29	0.3196670552323937
1101	30-31	0.3557533078310655
1101	32-33	0.36437437277977835
1101	34-35	0.35302880074010545
1101	36-37	0.28692437867270115
1101	38-39	0.3149371850867553
1101	40-41	0.24786353733765054
1101	42-43	0.2679543563321545
1101	44-45	0.26049025621229305
1101	46-47	0.2190602689003498
1101	48-49	0.25084709870301936
1101	50-51	0.23147785857882042
1101	52-53	0.1819860852007693
1101	54-55	0.24037979587890135
1101	56-57	0.11939054504053814
1101	58-59	0.18563042892233028
1101	60-61	0.27963446737678765
1101	62-63	0.266891748282184
1101	64-65	0.21289159443266925
1101	66-67	0.15992753932133041
1101	68-69	0.1327698212532482
1101	70-71	0.19016788483645541
1101	72-73	0.12027694201032091
1101	74-75	0.14998641141751534
1101	76-77	0.1556748761488258
1101	78-79	0.12917950913048415
1101	80-81	0.10505929775272449
1101	82-83	0.12509199202205679
1101	84-85	0.1722425916942747
1101	86-87	0.16039057051457206
1101	88-89	0.11284852902142717
1101	90-91	0.07791108401831437
1101	92-93	0.12496483729848862
1101	94-95	0.09041780667241284
1101	96-97	0.03643646136296752
1101	98-99	0.06734373059224197
1101	100	0.03407559546997874
1102	1	-0.06906275069216505
1102	2	0.02039820807731374
1102	3	0.023195633033321172
1102	4	0.1432927038920937
1102	5	0.08503476443043922
1102	6	0.1147935791963306
1102	7	0.05016089245865629
1102	8	0.036035532128529724
1102	9	1.3924211842919476E-4
1102	10-11	0.07132998381261757
1102	12-13	-0.10212321195335505
1102	14-15	1.1693147838798268E-4
1102	16-17	0.0683269332412948
1102	18-19	0.021756149519205792
1102	20-21	-0.033942494295203574
1102	22-23	-0.011966042984916214
1102	24-25	0.0062429260797856045
1102	26-27	0.06700725708688537
1102	28-29	0.019047310282410024
1102	30-31	0.08684687542817926
1102	32-33	0.0709891970080534
1102	34-35	0.09104071095541144
1102	36-37	-0.03977142319805438
1102	38-39	0.002246968900088575
1102	40-41	0.042781525309216306
1102	42-43	0.050260298140557325
1102	44-45	-0.12456523120514618
1102	46-47	-0.14499614946443273
1102	48-49	-0.12859758381437558
1102	50-51	-0.22694625443523364
1102	52-53	-0.17292008121714986
1102	54-55	-0.1479821062090778
1102	56-57	-0.2305957862880561
1102	58-59	-0.22535504534362616
1102	60-61	-0.17413202738478617
1102	62-63	-0.0035653764204255367
1102	64-65	1.119900170465371E-5
1102	66-67	0.015543590035790089
1102	68-69	-0.10928683354260471
1102	70-71	-0.051560795730381415
1102	72-73	-0.1272123816490378
1102	74-75	-0.04572347287902545
1102	76-77	-0.061569445811187506
1102	78-79	-0.0836040234452824
1102	80-81	-0.16219898841753633
1102	82-83	-0.11275788902355899
1102	84-85	-0.1542907376985383
1102	86-87	-0.1905673447544416
1102	88-89	-0.21893977650663032
1102	90-91	-0.1975652507089798
1102	92-93	-0.2002849669070308
1102	94-95	-0.21110761705640257
1102	96-97	-0.1575582632687187
1102	98-99	-0.2218542508774739
1102	100	-0.32660857694738255
1103	1	-0.47189569006442866
1103	2	-0.4200291346359961
1103	3	-0.4060225786345413
1103	4	-0.3777322115038615
1103	5	-0.3964979467701184
1103	6	-0.3962070719769386
1103	7	-0.3879777962588946
1103	8	-0.39320222219640044
1103	9	-0.39040054546332925
1103	10-11	-0.3997806731264859
1103	12-13	-0.3345579808763546
1103	14-15	-0.3272125420558538
1103	16-17	-0.304736572189249
1103	18-19	-0.351379298250059
1103	20-21	-0.4600559968540239
1103	22-23	-0.41162315140265804
1103	24-25	-0.3555250354495243
1103	26-27	-0.348025297847002
1103	28-29	-0.3932631464563343
1103	30-31	-0.41222298360361975
1103	32-33	-0.4102056574550943
1103	34-35	-0.4330222298266122
1103	36-37	-0.5227966861847833
1103	38-39	-0.4734941043199754
1103	40-41	-0.4041350984058667
1103	42-43	-0.4492636666020928
1103	44-45	-0.5518182869830213
1103	46-47	-0.6553186256831864
1103	48-49	-0.5220289014444646
1103	50-51	-0.7305311012677933
1103	52-53	-0.727733504784851
1103	54-55	-0.6415273978086447
1103	56-57	-0.6923761671013438
1103	58-59	-0.6576585353489008
1103	60-61	-0.6333436676477291
1103	62-63	-0.6559213854518191
1103	64-65	-0.6690812310531058
1103	66-67	-0.6723044948956982
1103	68-69	-0.7923878056779046
1103	70-71	-0.791992986812815
1103	72-73	-0.7725870748032158
1103	74-75	-0.7867590994412303
1103	76-77	-0.7681267904563889
1103	78-79	-0.7990292589827845
1103	80-81	-0.786557530535319
1103	82-83	-0.8101410377334837
1103	84-85	-0.7476434744537244
1103	86-87	-0.8319023021671121
1103	88-89	-0.8597029993145497
1103	90-91	-0.8667211198089788
1103	92-93	-0.8894653090825457
1103	94-95	-1.056212479821447
1103	96-97	-1.0158674728577495
1103	98-99	-1.0133489061099432
1103	100	-1.0114411627616526
1104	1	-0.22703533764823547
1104	2	-0.12062224597739757
1104	3	-0.17736240938344494
1104	4	-0.03145788849150932
1104	5	-0.16330036978481388
1104	6	-0.2749969924678197
1104	7	-0.2587812574322399
1104	8	-0.22603354335367953
1104	9	-0.2356046949745334
1104	10-11	-0.2812700292115551
1104	12-13	-0.30393404827039205
1104	14-15	-0.3213131088413945
1104	16-17	-0.30256257427077315
1104	18-19	-0.20784925393124354
1104	20-21	-0.2258696778663989
1104	22-23	-0.14217576637630458
1104	24-25	-0.12832762132722308
1104	26-27	-0.20782580182989818
1104	28-29	-0.15282887487596497
1104	30-31	-0.12794506347565004
1104	32-33	-0.1927794654411059
1104	34-35	-0.20976517066384304
1104	36-37	-0.37505793479366645
1104	38-39	-0.29853570317354183
1104	40-41	-0.24363142041650576
1104	42-43	-0.4301083752465189
1104	44-45	-0.39841201482407484
1104	46-47	-0.633846775511195
1104	48-49	-0.6056128690324982
1104	50-51	-0.7059215727412962
1104	52-53	-0.6635677147152492
1104	54-55	-0.512942524806455
1104	56-57	-0.5869667730297792
1104	58-59	-0.5881559330733594
1104	60-61	-0.7077775235184376
1104	62-63	-0.6634272501929814
1104	64-65	-0.5627914843943174
1104	66-67	-0.4874971584754064
1104	68-69	-0.5211435360942858
1104	70-71	-0.5318405054016893
1104	72-73	-0.655588379566364
1104	74-75	-0.6815662982831938
1104	76-77	-0.6081706742257502
1104	78-79	-0.5406911382953581
1104	80-81	-0.5773574444187197
1104	82-83	-0.6643484991641415
1104	84-85	-0.5958869524041965
1104	86-87	-0.5914519302036432
1104	88-89	-0.6746641594642817
1104	90-91	-0.7212197639187501
1104	92-93	-0.734969602456065
1104	94-95	-0.7345045154862149
1104	96-97	-0.7775105968972547
1104	98-99	-0.6662963731255367
1104	100	-0.6409030790344517
1105	1	-0.14175787741008605
1105	2	-0.06151882345488957
1105	3	-0.0374392991791872
1105	4	0.15672623591502344
1105	5	0.04741687489683244
1105	6	0.012893817688542697
1105	7	0.004180040657054462
1105	8	0.00628135645603578
1105	9	-0.05909764750075652
1105	10-11	-0.10283175678938505
1105	12-13	0.013649258415995291
1105	14-15	0.07126688805333714
1105	16-17	-0.06473864902940107
1105	18-19	0.02102668803986063
1105	20-21	-0.019720532129852586
1105	22-23	-0.03575372226950435
1105	24-25	-0.11919989034591794
1105	26-27	-0.08576915227151005
1105	28-29	-0.148364871261073
1105	30-31	-0.15285426962266513
1105	32-33	-0.13646675075365522
1105	34-35	-0.17509471815263566
1105	36-37	-0.2405996461590192
1105	38-39	-0.2129786516752361
1105	40-41	-0.21447912000500224
1105	42-43	-0.24512969059804135
1105	44-45	-0.3043449501613793
1105	46-47	-0.2820096175541522
1105	48-49	-0.3321074104053352
1105	50-51	-0.3048993712599426
1105	52-53	-0.2358368506990871
1105	54-55	-0.37066748324529897
1105	56-57	-0.40273156362002993
1105	58-59	-0.26915883586693923
1105	60-61	-0.3415661199776423
1105	62-63	-0.22161987522943605
1105	64-65	-0.1945798011156441
1105	66-67	-0.21600539468852276
1105	68-69	-0.1801737839980433
1105	70-71	-0.1739206980982786
1105	72-73	-0.20929933410441492
1105	74-75	-0.13627654599928718
1105	76-77	-0.12294883901335041
1105	78-79	-0.12079795623159839
1105	80-81	-0.07280543626585612
1105	82-83	-0.14346004845306126
1105	84-85	-0.11265033242941414
1105	86-87	-0.1125129812351311
1105	88-89	-0.10095517247458474
1105	90-91	-0.14175089641289773
1105	92-93	-0.17774048232990936
1105	94-95	-0.19739284421516246
1105	96-97	-0.15877609115727864
1105	98-99	-0.1597115737707533
1105	100	-0.18097289034773922
1106	1	-0.3697379608340974
1106	2	-0.30059998276845334
1106	3	-0.2963178956175483
1106	4	-0.23669413862258182
1106	5	-0.19107684304977113
1106	6	-0.14532012636937708
1106	7	-0.2673856810387889
1106	8	-0.12709950545475834
1106	9	-0.21075168855139736
1106	10-11	-0.1773375985439145
1106	12-13	-0.19102513979165536
1106	14-15	-0.19074106102718247
1106	16-17	-0.2943440362786802
1106	18-19	-0.29508921002241095
1106	20-21	-0.3184364422964663
1106	22-23	-0.20344301516305308
1106	24-25	-0.18984694487907205
1106	26-27	-0.29294998108582604
1106	28-29	-0.32581660309154614
1106	30-31	-0.2763508380142312
1106	32-33	-0.30300454429622903
1106	34-35	-0.21119806184238143
1106	36-37	-0.2764083521698595
1106	38-39	-0.35780361629684165
1106	40-41	-0.3821430191795301
1106	42-43	-0.3715326759400881
1106	44-45	-0.41862613791590775
1106	46-47	-0.33211692717850383
1106	48-49	-0.29760570500548766
1106	50-51	-0.3645942205813739
1106	52-53	-0.42214774571905167
1106	54-55	-0.3768323375763458
1106	56-57	-0.4810880807134623
1106	58-59	-0.5304131205279887
1106	60-61	-0.42868047226780703
1106	62-63	-0.4348208494453587
1106	64-65	-0.35616437341558793
1106	66-67	-0.35047120324500014
1106	68-69	-0.34556815480305403
1106	70-71	-0.27862298707400157
1106	72-73	-0.26251000307796346
1106	74-75	-0.3887160006719945
1106	76-77	-0.3464056662346273
1106	78-79	-0.34671483525323765
1106	80-81	-0.3231325920127297
1106	82-83	-0.3442940698856418
1106	84-85	-0.23416245221666543
1106	86-87	-0.3210553718873079
1106	88-89	-0.4207893811153056
1106	90-91	-0.5094576615959063
1106	92-93	-0.5041373096950856
1106	94-95	-0.4802037511560684
1106	96-97	-0.5263156810233234
1106	98-99	-0.6132905504236277
1106	100	-0.5798501634911872
1107	1	-0.28776279288958406
1107	2	-0.2887963815260619
1107	3	-0.37574963364946257
1107	4	-0.24796864659285944
1107	5	-0.12399193085307303
1107	6	-0.23105297873905073
1107	7	-0.23282234092658172
1107	8	-0.23654824679049113
1107	9	-0.16935834864284516
1107	10-11	-0.2633780149523659
1107	12-13	-0.21906905744263128
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2216	66-67	0.39122157016971926
2216	68-69	0.46459625804935456
2216	70-71	0.4205397429288027
2216	72-73	0.37006109153549005
2216	74-75	0.4298141976732026
2216	76-77	0.3882167157018621
2216	78-79	0.3529325201910112
2216	80-81	0.3604459116181076
2216	82-83	0.3515110685881204
2216	84-85	0.36895684677997664
2216	86-87	0.2980212600001373
2216	88-89	0.3684561011292331
2216	90-91	0.4321250553391458
2216	92-93	0.4129488455718082
2216	94-95	0.4074593372370394
2216	96-97	0.39911652593523783
2216	98-99	0.3841077391177983
2216	100	0.34933382149815273
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	73243.0
3	15942.0
4	12681.0
5	11491.0
6	7214.0
7	5488.0
8	5795.0
9	6061.0
10	6288.0
11	6595.0
12	7548.0
13	8567.0
14	8999.0
15	9107.0
16	8930.0
17	8653.0
18	8657.0
19	8061.0
20	7921.0
21	8010.0
22	9796.0
23	15800.0
24	26727.0
25	33925.0
26	38407.0
27	27735.0
28	24585.0
29	29417.0
30	34492.0
31	38451.0
32	49122.0
33	66105.0
34	93087.0
35	154748.0
36	246122.0
37	434184.0
38	636794.0
39	308977.0
40	1241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.0522527285532	22.844941873648523	13.79475553099159	21.30804986680669
2	14.73475843232446	23.991370226481568	39.3061790058165	21.96769233537747
3	17.000996670831462	30.822645385158083	30.01191045640485	22.164447487605607
4	11.348408023607409	20.716228413430375	38.83580990101861	29.09955366194361
5	11.552474067437661	42.33954008323217	33.63199859213234	12.475987257197824
6	26.122379675832892	41.84951838371893	18.80057370466994	13.227528235778237
7	24.118324131668178	37.367083914471856	22.40225586289033	16.112336090969638
8	23.76547636215756	38.22154612418321	20.662913417061716	17.350064096597507
9	24.950797132392562	20.263632300089757	20.912347193489204	33.87322337402848
10-11	21.683876076270593	31.163067379173242	28.39542596984205	18.757630574714113
12-13	22.56228544216741	29.71847455548351	27.73003056593929	19.989209436409784
14-15	20.75267958085483	30.827324334149626	25.51008912627487	22.909906958720672
16-17	19.69408862154123	33.61633604631375	26.383907634561776	20.305667697583246
18-19	19.8301031912979	32.43441856544501	28.17259658916017	19.56288165409692
20-21	21.861486907581234	31.22151563963169	27.37179241333244	19.545205039454636
22-23	21.393664373847965	31.31391375283739	27.49932286077938	19.793099012535265
24-25	20.794069149972085	31.406938966695396	27.537045341280546	20.261946542051966
26-27	20.320891506454625	31.56587720212905	27.55185692558275	20.561374365833576
28-29	20.473018483052208	31.326268657431932	27.920111315386404	20.280601544129457
30-31	20.731126553826705	31.67139696382042	27.471100840826303	20.126375641526575
32-33	20.294550404038507	31.51732070154524	27.895276382953476	20.292852511462772
34-35	20.819576283188457	31.181044544377883	27.741643846725907	20.257735325707753
36-37	20.839897540435047	31.222099206461777	27.395188108233803	20.54281514486937
38-39	20.5426801800677	31.38477734750729	27.74077362789271	20.3317688445323
40-41	20.47370380757648	31.129452646398693	27.883228621584284	20.513614924440546
42-43	20.401969415214246	31.188506963609125	27.874332819345288	20.53519080183134
44-45	20.627750998110862	31.22141119610281	27.710722060088035	20.440115745698296
46-47	20.670595064824962	31.34976394053307	27.57778655845393	20.401854436188035
48-49	20.557021785551825	31.1045483140375	27.7156824768457	20.62274742356498
50-51	20.539449383670487	31.091174417106405	27.55261099774364	20.81676520147947
52-53	20.512898881899137	31.207101861224128	27.62229122081768	20.65770803605906
54-55	20.636986165911182	31.43940566815886	27.477599587520373	20.446008578409582
56-57	20.633159292399554	31.171018888548414	27.314904890072157	20.88091692897987
58-59	20.651568520593173	30.87763717463326	27.614121080296684	20.85667322447688
60-61	20.779210661314064	31.22122402471318	27.364703974021115	20.63486133995164
62-63	20.45818448825925	31.163404525955574	27.61235589226746	20.766055093517718
64-65	20.6129605418886	31.062054114951575	27.529131406187734	20.79585393697209
66-67	20.446232545819875	30.995076284727336	27.477063844500965	21.081627324951825
68-69	20.780278155415633	31.33626938720396	27.227937948163362	20.65551450921705
70-71	20.78446928818237	31.0203170163226	27.324476434901634	20.870737260593394
72-73	20.60656817919891	31.102028864738955	27.530338495659624	20.761064460402515
74-75	20.555218428421163	31.25006741432987	27.471948647658557	20.722765509590413
76-77	20.633966238193587	31.133937173483954	27.30354803197957	20.92854855634289
78-79	20.732179896513482	31.056741804654013	27.533490097190395	20.677588201642106
80-81	20.667769234288162	31.15019883573174	27.409466166962087	20.77256576301801
82-83	20.54147437359838	31.099877062951197	27.710078720463844	20.648569842986575
84-85	20.48032231913798	31.008473902466015	27.47783436635115	21.033369412044856
86-87	20.75574066860436	30.986985487270147	27.37614239055524	20.88113145357025
88-89	20.613191424501327	30.9131748638541	27.614130127532967	20.859503584111607
90-91	20.565023351365994	30.947536685086867	27.385152410637254	21.10228755290989
92-93	20.642256993881404	31.01792594084024	27.367201344845054	20.972615720433303
94-95	20.565830905094547	30.850164550297098	27.584226661542534	20.99977788306582
96-97	20.60911925113096	31.169792280883417	27.268307454334234	20.952781013651393
98-99	20.406896606831086	31.08303335637263	27.355441735218562	21.154628301577727
100	20.403114961082963	31.28909683709185	27.230657354280872	21.07713084754431
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	33012.0
1	23745.5
2	11979.5
3	7658.5
4	4657.5
5	3047.0
6	2422.5
7	2387.0
8	2728.0
9	2758.5
10	2154.0
11	1571.5
12	1413.5
13	1360.0
14	1381.0
15	1483.5
16	1637.5
17	1921.5
18	2388.0
19	3042.0
20	4014.5
21	5457.5
22	7890.5
23	12126.5
24	16335.5
25	19890.5
26	25246.0
27	32238.0
28	41478.0
29	49961.5
30	53785.0
31	58018.5
32	63860.0
33	69162.0
34	74369.5
35	78635.0
36	80768.5
37	81719.5
38	82092.0
39	81886.5
40	82567.0
41	81958.0
42	80188.0
43	79296.0
44	78801.0
45	77946.0
46	77146.5
47	75468.5
48	72371.5
49	70815.5
50	70879.5
51	67501.5
52	61253.5
53	56304.0
54	54017.5
55	53669.0
56	49109.5
57	42654.0
58	38900.0
59	35435.5
60	30910.0
61	25683.0
62	21412.0
63	17320.0
64	13653.5
65	11274.5
66	9258.0
67	7354.0
68	5665.5
69	4343.0
70	3290.5
71	2694.5
72	2206.5
73	1720.5
74	1456.0
75	1232.0
76	914.5
77	694.0
78	568.5
79	404.0
80	289.0
81	210.0
82	151.0
83	112.5
84	84.5
85	81.0
86	81.0
87	63.0
88	53.0
89	52.5
90	42.5
91	40.5
92	32.5
93	22.0
94	18.0
95	16.5
96	14.5
97	12.5
98	15.5
99	18.0
100	25.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17824593228011879
2	0.17249735126145424
3	0.18531189644889393
4	0.1754514831738235
5	0.18886483888404076
6	0.07728647813982306
7	0.07892322690208171
8	0.06838416170119675
9	0.06207669086127316
10-11	0.07497107745174984
12-13	0.07397305991378725
14-15	0.07089916589686247
16-17	0.06754582696930816
18-19	0.0718373023825473
20-21	0.0733742493910097
22-23	0.07137821431508452
24-25	0.07119857115825125
26-27	0.07493115675023133
28-29	0.07217662834545459
30-31	0.07792520936411912
32-33	0.07437226692897228
34-35	0.07407286166758352
36-37	0.08483149072682025
38-39	0.0805799360150996
40-41	0.07600901569123093
42-43	0.079422235671063
44-45	0.08934252999841115
46-47	0.07964179952941476
48-49	0.08533049949580154
50-51	0.0815779535530622
52-53	0.0805000946120626
54-55	0.08201708126976574
56-57	0.07644814340793447
58-59	0.08375363178582065
60-61	0.08024061005219232
62-63	0.07201694553938058
64-65	0.07459183078732406
66-67	0.07491119639947208
68-69	0.07317464588341718
70-71	0.06864364626106702
72-73	0.06664761118514184
74-75	0.07141813501660302
76-77	0.07706691428147128
78-79	0.0733742493910097
80-81	0.08065977741813661
82-83	0.082196724426599
84-85	0.08403307669645017
86-87	0.07948211672334075
88-89	0.08161787425458071
90-91	0.08014080829839607
92-93	0.07097900729989948
94-95	0.07105884870293648
96-97	0.07097900729989948
98-99	0.07968172023093327
100	0.08806506754981905
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2504966.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.83391703119445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.42128490813674	46.922526546731156
2	13.373586969241613	16.00388186254132
3	3.9211458981472673	7.0385255501085595
4	1.5983500356007527	3.8254217366776846
5	0.7541710632062898	2.2562504411606423
6	0.41261840420309936	1.4813145217579262
7	0.278198815112629	1.1652007375137952
8	0.18659834637684947	0.8931927980216395
9	0.14298446602234752	0.7699788609057705
>10	0.786316553294194	8.95025012707479
>50	0.07941560074165602	3.297367770246874
>100	0.043060081701103266	4.6351184685940705
>500	0.0015348158516187336	0.6558148600080643
>1k	6.673112398313227E-4	0.6502648895012164
>5k	0.0	0.0
>10k+	6.673112398313228E-5	1.4548908291566574
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	36438	1.4546305219312357	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2866	0.11441273055203145	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2669	0.10654835235288623	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.984140303700729E-5	0.0
5	0.0	0.0	0.0	7.984140303700729E-5	0.0
6	0.0	0.0	0.0	7.984140303700729E-5	0.0
7	0.0	0.0	0.0	1.1976210455551093E-4	0.0
8	0.0	0.0	0.0	1.1976210455551093E-4	0.0
9	0.0	0.0	0.0	1.1976210455551093E-4	0.0
10-11	0.0	0.0	0.0	1.5968280607401458E-4	0.0
12-13	0.0	0.0	0.0	2.3952420911102187E-4	0.0
14-15	0.0	0.0	0.0	2.794449106295255E-4	1.9960350759251822E-5
16-17	0.0	0.0	0.0	2.794449106295255E-4	3.9920701518503644E-5
18-19	0.0	0.0	0.0	2.9940526138877736E-4	3.9920701518503644E-5
20-21	0.0	0.0	0.0	4.3912771670354003E-4	3.9920701518503644E-5
22-23	0.0	0.0	0.0	9.780571872033393E-4	3.9920701518503644E-5
24-25	0.0	0.0	0.0	0.0016367487622586494	3.9920701518503644E-5
26-27	0.0	0.0	0.0	0.0021956385835177	3.9920701518503644E-5
28-29	0.0	0.0	0.0	0.0038922683980541052	3.9920701518503644E-5
30-31	0.0	0.0	0.0	0.008682752580274542	3.9920701518503644E-5
32-33	0.0	0.0	0.0	0.01662697218245677	3.9920701518503644E-5
34-35	0.0	0.0	0.0	0.027984411764471057	3.9920701518503644E-5
36-37	0.0	0.0	0.0	0.04517027376818687	3.9920701518503644E-5
38-39	0.0	0.0	0.0	0.07103888835217723	3.9920701518503644E-5
40-41	0.0	0.0	0.0	0.09882369660905577	3.9920701518503644E-5
42-43	0.0	0.0	0.0	0.1256504080294902	3.9920701518503644E-5
44-45	0.0	0.0	0.0	0.1604612597536254	3.9920701518503644E-5
46-47	0.0	0.0	0.0	0.20463351598384968	3.9920701518503644E-5
48-49	0.0	0.0	0.0	0.2535962563962944	3.9920701518503644E-5
50-51	0.0	0.0	0.0	0.3034771729436647	3.9920701518503644E-5
52-53	0.0	0.0	0.0	0.35190098388560964	3.9920701518503644E-5
54-55	0.0	0.0	0.0	0.40816921267594053	3.9920701518503644E-5
56-57	0.0	0.0	0.0	0.47689270034004455	3.9920701518503644E-5
58-59	0.0	0.0	0.0	0.5564147377649038	3.9920701518503644E-5
60-61	0.0	0.0	0.0	0.6442003604040933	3.9920701518503644E-5
62-63	0.0	0.0	0.0	0.7319460623417643	3.9920701518503644E-5
64-65	0.0	0.0	0.0	0.8245820502154521	3.9920701518503644E-5
66-67	0.0	0.0	0.0	0.9343040983390593	3.9920701518503644E-5
68-69	0.0	0.0	0.0	1.0620104224967524	3.9920701518503644E-5
70-71	0.0	0.0	0.0	1.2047069700746436	3.9920701518503644E-5
72-73	0.0	0.0	0.0	1.358261948465568	3.9920701518503644E-5
74-75	0.0	0.0	0.0	1.5100005349374004	3.9920701518503644E-5
76-77	0.0	0.0	0.0	1.6806814942797628	3.9920701518503644E-5
78-79	0.0	0.0	0.0	1.882021552388336	3.9920701518503644E-5
80-81	0.0	0.0	0.0	2.1115256654182133	3.9920701518503644E-5
82-83	0.0	0.0	0.0	2.352606781888457	3.9920701518503644E-5
84-85	0.0	0.0	0.0	2.596542228517273	3.9920701518503644E-5
86-87	0.0	0.0	0.0	2.8530327357736596	3.9920701518503644E-5
88	0.0	0.0	0.0	3.062915824007192	3.9920701518503644E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3755	0.0	52.366913	1
GTATCAA	7080	0.0	41.12899	1
ATCAACG	8430	0.0	33.08961	3
TCAACGC	8550	0.0	32.515163	4
CAACGCA	9100	0.0	30.601654	5
TATCAAC	9425	0.0	30.545227	2
AACGCAG	9870	0.0	28.328379	6
GTACATG	16290	0.0	27.80967	1
CATGGGT	7090	0.0	27.268433	4
TACATGG	16220	0.0	26.768505	2
CGCAGAG	10835	0.0	25.50228	8
ACATGGG	16465	0.0	25.455437	3
ACGCAGA	11170	0.0	24.94779	7
ATGGGTG	3815	0.0	24.537092	5
GCAGAGT	11840	0.0	22.464876	9
GTGGTAT	1845	0.0	21.672707	1
TGGTATC	1715	0.0	21.12029	2
ATGGGTA	3120	0.0	20.95679	5
TACACGC	345	3.6379788E-12	20.452045	5
TATAACG	140	5.1825953E-4	20.160275	2
>>END_MODULE