##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431396_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2504966
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60058340113199	34.0	31.0	34.0	31.0	34.0
2	32.733105758720875	34.0	31.0	34.0	31.0	34.0
3	32.813458545944336	34.0	31.0	34.0	31.0	34.0
4	36.284823027538096	37.0	35.0	37.0	35.0	37.0
5	36.209457134348334	37.0	37.0	37.0	35.0	37.0
6	36.267738963323254	37.0	37.0	37.0	35.0	37.0
7	36.22123573733136	37.0	37.0	37.0	35.0	37.0
8	36.24022082535252	37.0	37.0	37.0	35.0	37.0
9	38.02718120724992	39.0	39.0	39.0	35.0	39.0
10-11	38.00235312575101	39.0	39.0	39.0	35.0	39.0
12-13	37.989226400677694	39.0	39.0	39.0	35.0	39.0
14-15	39.41046884468691	41.0	39.0	41.0	36.0	41.0
16-17	39.44276529102591	41.0	39.0	41.0	36.0	41.0
18-19	39.53784482503954	41.0	39.5	41.0	36.5	41.0
20-21	39.529427944331374	41.0	40.0	41.0	36.0	41.0
22-23	39.444487869296424	41.0	39.0	41.0	36.0	41.0
24-25	39.379814536404886	41.0	39.0	41.0	36.0	41.0
26-27	39.24305539476384	41.0	39.0	41.0	35.5	41.0
28-29	39.14419057983222	41.0	39.0	41.0	35.0	41.0
30-31	38.905849420710695	41.0	38.5	41.0	35.0	41.0
32-33	38.614064422431284	41.0	38.0	41.0	35.0	41.0
34-35	38.44255650575697	40.5	38.0	41.0	35.0	41.0
36-37	38.28043434521666	40.0	38.0	41.0	35.0	41.0
38-39	38.12363002132564	40.0	38.0	41.0	34.0	41.0
40-41	37.963928053314895	40.0	37.5	41.0	34.0	41.0
42-43	37.75521084916921	40.0	37.0	41.0	33.5	41.0
44-45	37.45392292749682	40.0	37.0	41.0	33.0	41.0
46-47	36.99782631780232	40.0	37.0	41.0	31.5	41.0
48-49	36.57902382706991	40.0	36.5	41.0	30.5	41.0
50-51	36.38455671653827	40.0	36.0	41.0	30.0	41.0
52-53	36.31474119808413	40.0	36.0	41.0	30.0	41.0
54-55	36.162228349606345	40.0	35.0	41.0	30.0	41.0
56-57	36.00549009447633	40.0	35.0	41.0	29.5	41.0
58-59	35.81361723073287	39.5	35.0	41.0	29.0	41.0
60-61	35.6455596602908	39.0	35.0	41.0	28.5	41.0
62-63	35.47369904421856	39.0	35.0	41.0	28.5	41.0
64-65	35.23479280756705	39.0	35.0	41.0	28.0	41.0
66-67	34.966297346950014	38.0	35.0	41.0	28.0	41.0
68-69	34.67216541062833	37.0	35.0	40.0	28.0	41.0
70-71	34.37248190194997	37.0	35.0	40.0	27.0	41.0
72-73	34.052570573812176	36.0	35.0	39.0	26.0	41.0
74-75	33.72471123360556	36.0	34.5	39.0	26.0	41.0
76-77	33.40318032260717	35.0	34.0	38.5	26.0	40.5
78-79	33.09259786360374	35.0	34.0	37.0	26.0	39.5
80-81	32.836475824422365	35.0	34.0	37.0	26.0	39.0
82-83	32.64677624366958	35.0	34.0	36.5	26.0	39.0
84-85	32.39557702579596	35.0	34.0	36.0	26.0	38.0
86-87	32.16633818582767	35.0	34.0	36.0	25.0	37.0
88-89	31.94507909488592	35.0	34.0	36.0	25.0	37.0
90-91	31.769236588440723	35.0	34.0	35.0	24.5	36.5
92-93	31.61488718808958	35.0	34.0	35.0	24.0	36.0
94-95	31.476870544350703	35.0	34.0	35.0	23.5	36.0
96-97	31.339765090623985	35.0	34.0	35.0	21.5	36.0
98-99	31.22323596408095	35.0	34.0	35.0	20.0	36.0
100	31.12196413045127	35.0	34.0	35.0	18.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.010557458566069045
1101	2	0.0644646328999201
1101	3	0.03194648094839181
1101	4	0.09048411352087271
1101	5	0.00990530180731497
1101	6	0.05976397802177047
1101	7	0.06877754278097115
1101	8	0.051234786452404535
1101	9	0.05098108348047958
1101	10-11	0.0732061463197411
1101	12-13	0.03357899945275733
1101	14-15	0.053755029890652395
1101	16-17	0.05816401652397474
1101	18-19	0.010040926662838956
1101	20-21	0.054601326831800634
1101	22-23	0.03513508775326102
1101	24-25	0.03268337035449065
1101	26-27	0.09324983581084467
1101	28-29	0.03990027287606068
1101	30-31	-0.003386008889961545
1101	32-33	-0.09481687174519493
1101	34-35	-0.033786389503262626
1101	36-37	-0.029237699597146616
1101	38-39	-0.025359631545043726
1101	40-41	-0.09133848983178439
1101	42-43	-0.14961541455500083
1101	44-45	-0.20530240089130558
1101	46-47	-0.30384646084817035
1101	48-49	-0.18579730875015343
1101	50-51	-0.26004865137422684
1101	52-53	-0.2246409171815742
1101	54-55	-0.1783827942232179
1101	56-57	-0.09859950674026408
1101	58-59	-0.12615314214198747
1101	60-61	-0.13152457832126174
1101	62-63	-0.17180463358158704
1101	64-65	-0.16439200591587166
1101	66-67	-0.19833433617952778
1101	68-69	-0.1610037187328217
1101	70-71	-0.19751156421985172
1101	72-73	-0.2641612242304916
1101	74-75	-0.2475854301960112
1101	76-77	-0.3060169237082988
1101	78-79	-0.24935707859949474
1101	80-81	-0.2127501594232939
1101	82-83	-0.2810288535651111
1101	84-85	-0.30849043165120804
1101	86-87	-0.25276870848587407
1101	88-89	-0.24010513017496748
1101	90-91	-0.2458310464917126
1101	92-93	-0.27470045785682373
1101	94-95	-0.29424371295571916
1101	96-97	-0.3340945613489197
1101	98-99	-0.24939858689531036
1101	100	-0.1983860758625653
1102	1	-0.022658742873204574
1102	2	0.01317859563247481
1102	3	-0.023978186200992013
1102	4	0.004251938207232797
1102	5	0.0380973429463225
1102	6	-0.008909643658959965
1102	7	0.006475886285770116
1102	8	0.0529180279224164
1102	9	0.05343745455904525
1102	10-11	0.03454309015258161
1102	12-13	0.003114634048536402
1102	14-15	-0.027855804563273523
1102	16-17	0.003034274972684159
1102	18-19	0.01267617262504217
1102	20-21	0.008619251262516059
1102	22-23	0.04201781794107262
1102	24-25	0.044607180441232686
1102	26-27	0.06920110145593839
1102	28-29	0.04890216432464456
1102	30-31	0.05060332215787611
1102	32-33	0.08715319017019851
1102	34-35	0.11282363221883429
1102	36-37	0.09573415032823362
1102	38-39	0.10720032589736661
1102	40-41	0.09888434645447575
1102	42-43	0.10053080328668074
1102	44-45	0.05916311711440159
1102	46-47	-0.025826497199439302
1102	48-49	0.09788185428385532
1102	50-51	0.10872146373814928
1102	52-53	0.07461506378572125
1102	54-55	0.05397250257166064
1102	56-57	0.045699401243723514
1102	58-59	0.12566981531519872
1102	60-61	0.12995245092230334
1102	62-63	0.10388426745723223
1102	64-65	0.06021625735012748
1102	66-67	0.09997348230052694
1102	68-69	0.02821103456613372
1102	70-71	0.03845418282844548
1102	72-73	0.027165966820589915
1102	74-75	0.07512303551408905
1102	76-77	0.045384964784894066
1102	78-79	0.0124700164014655
1102	80-81	0.044937101374955546
1102	82-83	8.62883674201953E-5
1102	84-85	0.011613302482054166
1102	86-87	-0.08085923203292822
1102	88-89	-0.06353478454229133
1102	90-91	0.03264463805012596
1102	92-93	0.05075422865035151
1102	94-95	0.08194073283506853
1102	96-97	0.049411120385304486
1102	98-99	0.04645862351356911
1102	100	0.04046250481215097
1103	1	-0.09660599410898385
1103	2	-0.13612752815608786
1103	3	-0.06422733270154879
1103	4	-0.015390669857318073
1103	5	-0.04286266397566152
1103	6	-0.025503895581714175
1103	7	-0.09081760489057444
1103	8	-0.021307892652345117
1103	9	-0.04411091165244585
1103	10-11	-0.011770993166265953
1103	12-13	-0.01351937450100138
1103	14-15	-0.01235057093528269
1103	16-17	-0.008449124584402057
1103	18-19	-0.037767229378601996
1103	20-21	-0.039601136204225895
1103	22-23	-0.037630481362924684
1103	24-25	-0.03834498405721831
1103	26-27	-0.04260061218148081
1103	28-29	-0.05816045497320488
1103	30-31	-0.07496559666326874
1103	32-33	-0.05758483752816801
1103	34-35	-0.04867964565164584
1103	36-37	-0.09559808453870033
1103	38-39	-0.13690935316297725
1103	40-41	-0.13234914578104906
1103	42-43	-0.07327733705048445
1103	44-45	-0.17332994412348768
1103	46-47	-0.18579347607622054
1103	48-49	-0.16042250693605808
1103	50-51	-0.1384340353939919
1103	52-53	-0.05038480568126147
1103	54-55	-0.039738938908151056
1103	56-57	-0.02273652233703416
1103	58-59	-0.0934181766171136
1103	60-61	-0.04967306104683189
1103	62-63	-0.1299287809212899
1103	64-65	-0.12475648362687508
1103	66-67	-0.054698592659740086
1103	68-69	-0.05496743688480166
1103	70-71	-0.07882973095411216
1103	72-73	-0.11416693935899502
1103	74-75	-0.0018219996440507202
1103	76-77	-7.354075400201054E-4
1103	78-79	0.025123029066151048
1103	80-81	-0.04389613486667798
1103	82-83	-0.0917111661642096
1103	84-85	0.017939640986234906
1103	86-87	0.039715344462024404
1103	88-89	-0.0010886853536078434
1103	90-91	0.003023899859822876
1103	92-93	0.00979550468143131
1103	94-95	-0.030036640945098725
1103	96-97	-0.02225650228030318
1103	98-99	-0.06046141643698988
1103	100	0.014507991465336545
1104	1	-0.11950036704367761
1104	2	-0.10107115636440511
1104	3	-0.12663925932990594
1104	4	-0.022220504424950605
1104	5	-0.07056997314057156
1104	6	-0.10480954889845151
1104	7	-0.08741451190658012
1104	8	-0.14719169399369747
1104	9	-0.10322891556189973
1104	10-11	-0.10183709397697527
1104	12-13	-0.07332114271721224
1104	14-15	-0.06048075127073105
1104	16-17	-0.03985219674571994
1104	18-19	-0.06366326403643541
1104	20-21	-0.015735571800277626
1104	22-23	-0.02853551252813702
1104	24-25	0.006927917436321707
1104	26-27	-0.05400436851612511
1104	28-29	-0.06752619283740557
1104	30-31	-0.08282388503265992
1104	32-33	-0.09172441706647305
1104	34-35	-0.05551747463582757
1104	36-37	-0.0763274876681237
1104	38-39	-0.06522040810246921
1104	40-41	-0.1452529521272794
1104	42-43	-0.15796996288911203
1104	44-45	-0.1129919178817218
1104	46-47	-0.19021806957872656
1104	48-49	-0.26648524934815043
1104	50-51	-0.3589059141971518
1104	52-53	-0.36750111179974
1104	54-55	-0.263220845684323
1104	56-57	-0.27932081676656395
1104	58-59	-0.19802805466694196
1104	60-61	-0.23741516637456783
1104	62-63	-0.28182507511878896
1104	64-65	-0.2514559013730633
1104	66-67	-0.3227810519058778
1104	68-69	-0.3337731602921181
1104	70-71	-0.31844730781300257
1104	72-73	-0.30692024244740423
1104	74-75	-0.29095721571825095
1104	76-77	-0.3437573787995305
1104	78-79	-0.28788016832863406
1104	80-81	-0.27600717768439864
1104	82-83	-0.29153908170003007
1104	84-85	-0.2456562401416278
1104	86-87	-0.22774767764309445
1104	88-89	-0.19140090264408371
1104	90-91	-0.19445733836003498
1104	92-93	-0.1789405483152322
1104	94-95	-0.16311894959873996
1104	96-97	-0.15164717528746863
1104	98-99	-0.19704803110648683
1104	100	-0.14044923468778592
1105	1	-0.1569859751312137
1105	2	-0.12550643553268515
1105	3	-0.08133572541073875
1105	4	-0.03207364057534079
1105	5	-0.11702082819574144
1105	6	-0.05505872526247657
1105	7	-0.0399330397757609
1105	8	-0.02063457080156894
1105	9	-0.04376863665385855
1105	10-11	-0.05006333887281755
1105	12-13	-0.11366545048399956
1105	14-15	-0.12882787576918275
1105	16-17	-0.10642163690783946
1105	18-19	-0.17942035010612756
1105	20-21	-0.1146867966870957
1105	22-23	-0.12924019392971076
1105	24-25	-0.1334140665121737
1105	26-27	-0.07128137102804999
1105	28-29	-0.10692546894968302
1105	30-31	-0.09975037217740379
1105	32-33	-0.1521034105701915
1105	34-35	-0.18599565632791837
1105	36-37	-0.06669797321126225
1105	38-39	-0.05410830490910712
1105	40-41	-0.1281288304682775
1105	42-43	-0.18012738699005126
1105	44-45	-0.13428667026018815
1105	46-47	-0.14043260858815643
1105	48-49	-0.126744635851324
1105	50-51	-0.19668444801002494
1105	52-53	-0.16691153637527378
1105	54-55	-0.03033342155845986
1105	56-57	-0.09798612843457732
1105	58-59	-0.05045980221787971
1105	60-61	-0.07530959787551694
1105	62-63	-0.09679925434543435
1105	64-65	-0.15522237097700753
1105	66-67	-0.11888032864859355
1105	68-69	-0.08759415532325221
1105	70-71	-0.09438346570713918
1105	72-73	0.03398199429733495
1105	74-75	0.01509793633350398
1105	76-77	-0.051705247858159
1105	78-79	-0.017888183669057867
1105	80-81	0.04505511222812686
1105	82-83	0.01850017250237812
1105	84-85	-0.04288366835213964
1105	86-87	-0.04215373094782393
1105	88-89	0.07603323250185667
1105	90-91	0.058691655338680704
1105	92-93	-0.025643350722795333
1105	94-95	-0.03328344661083804
1105	96-97	-0.06323431971944515
1105	98-99	-0.0890872454301217
1105	100	-0.030981339227061255
1106	1	-0.211995401138644
1106	2	-0.16261385392679273
1106	3	-0.132574113603674
1106	4	-0.07759117656070913
1106	5	-0.0447903694737235
1106	6	-0.09080165448721544
1106	7	-0.11337724772943858
1106	8	-0.07929469661051769
1106	9	-0.14283799917416928
1106	10-11	-0.14466986108025992
1106	12-13	-0.1504901168704862
1106	14-15	-0.20126123736007884
1106	16-17	-0.2561704083808678
1106	18-19	-0.17937866208404785
1106	20-21	-0.12960324359228537
1106	22-23	-0.1643891217434117
1106	24-25	-0.14444721420331064
1106	26-27	-0.19355632518885812
1106	28-29	-0.18494435538713105
1106	30-31	-0.22098873356585358
1106	32-33	-0.230034441793606
1106	34-35	-0.1724026454129941
1106	36-37	-0.09886550206523026
1106	38-39	-0.1471403107293412
1106	40-41	-0.1696505942257147
1106	42-43	-0.11872135369634407
1106	44-45	-0.10787804892328978
1106	46-47	-0.18137486651583146
1106	48-49	-0.15801901117641393
1106	50-51	-0.24667399426235903
1106	52-53	-0.18085684987271833
1106	54-55	-0.20806815227993525
1106	56-57	-0.21853713122548868
1106	58-59	-0.1881217630051779
1106	60-61	-0.17635914144457843
1106	62-63	-0.09344245157267039
1106	64-65	-0.07199646718280661
1106	66-67	-0.03384836712628214
1106	68-69	-0.0757419896551923
1106	70-71	-0.06251840426210009
1106	72-73	-0.00279509413500989
1106	74-75	-0.036433326085614226
1106	76-77	-0.028210273627848892
1106	78-79	0.02994257904398978
1106	80-81	-0.0736025066564423
1106	82-83	-0.06620343412521379
1106	84-85	-0.05757351158401747
1106	86-87	-0.06070627626608882
1106	88-89	-0.09470380044240301
1106	90-91	-0.13628124885773119
1106	92-93	-0.16971810729225467
1106	94-95	-0.17488915777110847
1106	96-97	-0.19290219667897546
1106	98-99	-0.14990350959928733
1106	100	-0.08197989785135107
1107	1	-0.21559265351114476
1107	2	-0.20901175949881434
1107	3	-0.15150364112296444
1107	4	-0.18972362194835313
1107	5	-0.23565401345089043
1107	6	-0.22424039084789626
1107	7	-0.16914567362084654
1107	8	-0.17641801690562886
1107	9	-0.14027960668671113
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2216	28-29	0.06588550449124853
2216	30-31	-0.10023445322790536
2216	32-33	-0.03671986199628918
2216	34-35	0.01489101398514947
2216	36-37	-0.027144271591446056
2216	38-39	-0.01741535035297659
2216	40-41	-0.013862483727692165
2216	42-43	0.04094171046548922
2216	44-45	0.1128702164297053
2216	46-47	0.07376026627093069
2216	48-49	-0.02224293309591019
2216	50-51	-0.025387592742106335
2216	52-53	-0.005402920435408021
2216	54-55	-0.01714368764113061
2216	56-57	-0.09615748936124646
2216	58-59	-0.03014659663240593
2216	60-61	-0.12003657842215887
2216	62-63	-0.10426146979250461
2216	64-65	-0.03770921720089149
2216	66-67	-0.05902728998260187
2216	68-69	-0.09487187527013674
2216	70-71	-0.08667086350980924
2216	72-73	-0.15960969883668952
2216	74-75	-0.11952171552474056
2216	76-77	-0.05807718275409002
2216	78-79	-0.1077410692163312
2216	80-81	-0.12198021075320753
2216	82-83	-0.08218680339548001
2216	84-85	-0.05277149759816524
2216	86-87	-0.08034737441264639
2216	88-89	-0.057795473341279546
2216	90-91	-0.03462365851577687
2216	92-93	-0.04511998844667531
2216	94-95	-0.14965592871791245
2216	96-97	-0.13684758502121497
2216	98-99	-0.05807781909064147
2216	100	-0.08990970401708509
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	54.0
3	323.0
4	72.0
5	136.0
6	308.0
7	714.0
8	1249.0
9	2018.0
10	2863.0
11	4037.0
12	6132.0
13	9103.0
14	9987.0
15	12794.0
16	17068.0
17	26462.0
18	31558.0
19	16310.0
20	9769.0
21	8241.0
22	8079.0
23	7984.0
24	8415.0
25	9546.0
26	11382.0
27	14431.0
28	17522.0
29	21617.0
30	27523.0
31	34826.0
32	43603.0
33	56803.0
34	80935.0
35	145804.0
36	219033.0
37	434771.0
38	745789.0
39	453146.0
40	4559.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.93808903486412	20.830959594608675	14.784063965787823	21.446887404739385
2	14.701544912981374	22.191566335285	40.64253337304271	22.464355378690918
3	16.518013263433897	29.411257471260697	32.18746057777217	21.883268687533235
4	10.791843082900126	18.860495511715527	39.88980289552832	30.457858509856024
5	10.788305676182686	39.43553292518251	35.40519998897681	14.37096140965799
6	25.966699747621323	40.479351815553585	18.90313082093729	14.650817615887801
7	23.331973368101604	36.118574064478324	23.724713229640642	16.824739337779434
8	25.149802432448187	34.207729765593626	21.34440148089834	19.29806632105985
9	26.36400109382766	15.381053228288618	22.563548792098892	35.69139688578483
10-11	22.607951565011263	27.585045066479946	29.55419354993241	20.25280981857638
12-13	24.73640854534723	26.3790259786013	29.714980106512446	19.16958536953902
14-15	22.007644015926765	26.69351599981796	29.46702669816676	21.831813286088515
16-17	18.705683031226773	29.95697346790336	30.0891109899296	21.24823251094027
18-19	18.629784973000614	26.934713046831604	35.17766419444268	19.25783778572511
20-21	20.394687991773143	25.888574934749613	35.17602634127569	18.540710732201553
22-23	19.84281613571769	25.9796695721636	35.46179995764411	18.7157143344746
24-25	19.37829857469038	26.194582797480763	35.32149856814338	19.105620059685478
26-27	18.99491988808624	26.32837710873747	35.29690740128253	19.37979560189376
28-29	19.127796022658206	26.13476159043372	35.66974701637488	19.067695370533194
30-31	19.447568245573486	26.443824873920192	35.18064752305525	18.927959357451073
32-33	19.04233328489556	26.33229165502297	35.50289987911847	19.122475180962997
34-35	19.522532240241695	26.17186252467121	35.11403847750688	19.19156675758021
36-37	19.640149624939617	26.251831388501873	34.63166635395001	19.476352632608496
38-39	19.419750457046966	26.477585586398394	34.77547062513885	19.32719333141579
40-41	19.37706427823342	26.32886772910482	34.68807804032118	19.605989952340586
42-43	19.436073814484928	26.488993517050503	34.411609308029625	19.663323360434944
44-45	19.744531309725147	26.62263277583375	33.919083484017435	19.713752430423664
46-47	19.957065268377004	26.95518699862074	33.13309766803129	19.954650064970966
48-49	20.07818873281438	27.046111831125753	32.517027686420306	20.358671749639566
50-51	20.163563392092055	27.189499718857956	32.130952910853125	20.515983978196868
52-53	20.155056863365047	27.464178954327267	32.010493614248894	20.37027056805879
54-55	20.21811196382206	27.770412367183084	31.88352511345527	20.127950555539588
56-57	20.186939989935812	27.343311956083255	31.83514932503873	20.634598728942205
58-59	20.220874359544	27.08090190097341	32.09838026933434	20.59984347014825
60-61	20.281524552058787	27.29895915358997	31.976410542000632	20.44310575235061
62-63	20.040440090381406	27.166099786821242	32.24684024367849	20.54661987911886
64-65	20.08306431346389	27.136644528292546	32.233500663592324	20.54679049465124
66-67	20.014698858044042	27.103384829237555	32.1186016603482	20.76331465237021
68-69	20.45377924819234	27.376238167238032	31.675304520526755	20.494678064042876
70-71	20.351210986675845	27.150097915676547	31.74226430226572	20.756426795381884
72-73	20.285700997799765	27.179639287869296	31.824780540539244	20.70987917379169
74-75	20.256922676486134	27.47820720204267	31.61173290069766	20.653137220773537
76-77	20.306281379100554	27.377296526499617	31.47907254890963	20.837349545490202
78-79	20.362965401098904	27.372867197522343	31.643073043987513	20.621094357391236
80-81	20.275686306524864	27.435114933500625	31.524061273115993	20.765137486858514
82-83	20.117108827175155	27.377896033230403	31.809947164297675	20.695047975296767
84-85	20.077997922903098	27.409734809856612	31.447099399845545	21.06516786739475
86-87	20.28574548144523	27.37063816171485	31.324654285573423	21.018962071266508
88-89	20.066744166921495	27.357323814002584	31.519642402326927	21.056289616748998
90-91	20.00940540739067	27.369216532758045	31.367911911732886	21.2534661481184
92-93	20.0013094133848	27.39650095201938	31.396838684861923	21.205350949733894
94-95	19.922844644156	27.258391997272597	31.526727522269944	21.292035836301462
96-97	19.925939040628734	27.510520612376187	31.217580615446096	21.345959731548984
98-99	19.76079063921367	27.380112381013593	31.277000176250287	21.58209680352245
100	19.828348830562035	27.44921683505232	31.19350218206729	21.528932152318355
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3769.0
1	2219.0
2	4830.0
3	7980.5
4	4227.5
5	1932.5
6	4290.0
7	10988.0
8	18938.5
9	19950.5
10	11932.5
11	4923.0
12	3742.5
13	3976.5
14	4334.5
15	4604.5
16	5035.5
17	5548.5
18	6106.0
19	6713.0
20	7586.5
21	8724.5
22	10888.5
23	14642.0
24	18526.0
25	21847.5
26	26866.0
27	33521.0
28	42255.0
29	50170.5
30	53524.5
31	57622.0
32	63156.5
33	67598.5
34	72345.0
35	77348.5
36	79192.0
37	79731.0
38	80786.5
39	80408.0
40	81022.5
41	80511.5
42	78550.5
43	78288.0
44	78845.5
45	77975.5
46	76444.5
47	74775.0
48	71324.5
49	69259.0
50	69909.0
51	66813.0
52	59775.5
53	54621.5
54	51925.5
55	50737.0
56	46067.5
57	39899.0
58	36532.0
59	33774.0
60	29515.5
61	24170.0
62	20079.5
63	16253.5
64	12565.5
65	10061.0
66	8203.0
67	6388.5
68	4781.5
69	3556.0
70	2654.5
71	2077.0
72	1664.0
73	1237.5
74	1085.5
75	883.5
76	565.5
77	456.5
78	365.0
79	208.0
80	109.5
81	58.0
82	29.5
83	19.0
84	12.0
85	6.0
86	3.5
87	2.5
88	2.0
89	2.0
90	1.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005069929092849963
2	0.003992070151850364
3	0.001357303851629124
4	0.0
5	0.0460285688508347
6	0.0
7	0.0
8	0.0
9	3.9920701518503644E-5
10-11	0.0
12-13	1.9960350759251822E-5
14-15	0.0
16-17	0.0
18-19	5.9881052277755467E-5
20-21	0.0
22-23	5.9881052277755467E-5
24-25	5.9881052277755467E-5
26-27	5.9881052277755467E-5
28-29	5.9881052277755467E-5
30-31	1.9960350759251822E-4
32-33	0.001357303851629124
34-35	8.782554334070801E-4
36-37	0.0014371452546661312
38-39	0.0010578985902403467
40-41	1.796431568332664E-4
42-43	4.1916736594428824E-4
44-45	5.9881052277755467E-5
46-47	3.9920701518503644E-5
48-49	5.9881052277755467E-5
50-51	1.796431568332664E-4
52-53	5.58889821259051E-4
54-55	5.58889821259051E-4
56-57	0.0017764712175734121
58-59	4.990087689812955E-4
60-61	0.001337343500869872
62-63	0.0010379382394810946
64-65	0.001576867709980894
66-67	3.7924666442578465E-4
68-69	1.3972245531476276E-4
70-71	1.796431568332664E-4
72-73	1.796431568332664E-4
74-75	3.9920701518503644E-4
76-77	5.788501720183028E-4
78-79	6.586915750553102E-4
80-81	6.986122765738138E-4
82-83	0.001237541747073613
84-85	0.0010978192917588502
86-87	0.0017764712175734121
88-89	0.001117779642518102
90-91	9.580968364440875E-4
92-93	0.0010978192917588502
94-95	7.984140303700729E-4
96-97	9.980175379625911E-5
98-99	2.1956385835177002E-4
100	7.984140303700729E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2504966.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.0315570854735
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.11742688240794	40.58711538710706
2	15.181125992583874	16.405197513801227
3	4.651179327574495	7.539313840578467
4	1.829043194812289	3.9530420716918813
5	0.890260772140184	2.4051087865425034
6	0.5215896807550249	1.6909381566545418
7	0.3222656576044675	1.2188760692880414
8	0.21672374677559536	0.936793719654663
9	0.16475548572796866	0.8011795889030091
>10	0.950416008705845	9.715641910194142
>50	0.09437605959075056	3.527722101574007
>100	0.05727638141396204	5.600703797926636
>500	0.002373873271782157	0.9693557029634857
>1k	7.418353974313662E-4	0.8006884519677716
>5k	1.4836707948627322E-4	0.48219273514078353
>10k+	2.9673415897254644E-4	3.3661301660118874
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31445	1.255306459249347	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24549	0.980013301577746	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17377	0.6937020302870378	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10609	0.42351872240980515	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6463	0.25800749391408906	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5567	0.2222385453535098	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4612	0.1841142754033388	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4415	0.17624989720419357	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	3.9920701518503644E-5	0.0
9	0.0	0.0	0.0	1.5968280607401458E-4	0.0
10-11	0.0	0.0	0.0	1.5968280607401458E-4	0.0
12-13	0.0	0.0	0.0	1.796431568332664E-4	0.0
14-15	0.0	0.0	0.0	1.9960350759251822E-4	0.0
16-17	0.0	0.0	0.0	1.9960350759251822E-4	0.0
18-19	0.0	0.0	0.0	2.1956385835177004E-4	0.0
20-21	0.0	0.0	0.0	3.592863136665328E-4	0.0
22-23	0.0	0.0	0.0	8.782554334070801E-4	0.0
24-25	0.0	0.0	0.0	0.0014770659561846348	0.0
26-27	0.0	0.0	0.0	0.0020758764789621893	0.0
28-29	0.0	0.0	0.0	0.00383238734577635	0.0
30-31	0.0	0.0	0.0	0.008722673281793046	0.0
32-33	0.0	0.0	0.0	0.016906417093086296	0.0
34-35	0.0	0.0	0.0	0.0285033808842116	0.0
36-37	0.0	0.0	0.0	0.045789044641723675	0.0
38-39	0.0	0.0	0.0	0.07195706448710282	0.0
40-41	0.0	0.0	0.0	0.10006123835612939	0.0
42-43	0.0	0.0	0.0	0.12722727573947112	1.9960350759251822E-5
44-45	0.0	0.0	0.0	0.1625770569341061	3.9920701518503644E-5
46-47	0.0	0.0	0.0	0.20738804438862646	3.9920701518503644E-5
48-49	0.0	0.0	0.0	0.25684979357005244	3.9920701518503644E-5
50-51	0.0	0.0	0.0	0.3078884104614593	3.9920701518503644E-5
52-53	0.0	0.0	0.0	0.35741004069516313	3.9920701518503644E-5
54-55	0.0	0.0	0.0	0.41477608877725286	3.9920701518503644E-5
56-57	0.0	0.0	0.0	0.4848369199422268	3.9920701518503644E-5
58-59	0.0	0.0	0.0	0.5661154682339002	3.9920701518503644E-5
60-61	0.0	0.0	0.0	0.6553981171800336	3.9920701518503644E-5
62-63	0.0	0.0	0.0	0.744920290335278	3.9920701518503644E-5
64-65	0.0	0.0	0.0	0.8397718771432426	3.9920701518503644E-5
66-67	0.0	0.0	0.0	0.9512105154321455	3.9920701518503644E-5
68-69	0.0	0.0	0.0	1.0816913283453746	3.9920701518503644E-5
70-71	0.0	0.0	0.0	1.2275416113432278	3.9920701518503644E-5
72-73	0.0	0.0	0.0	1.3843700872586693	3.9920701518503644E-5
74-75	0.0	0.0	0.0	1.5397015368671672	3.9920701518503644E-5
76-77	0.0	0.0	0.0	1.7132767470696209	3.9920701518503644E-5
78-79	0.0	0.0	0.0	1.9189681616437109	3.9920701518503644E-5
80-81	0.0	0.0	0.0	2.152863551840624	3.9920701518503644E-5
82-83	0.0	0.0	0.0	2.398695231791569	3.9920701518503644E-5
84-85	0.0	0.0	0.0	2.6486387439989203	3.9920701518503644E-5
86-87	0.0	0.0	0.0	2.911157277184601	3.9920701518503644E-5
88	0.0	0.0	0.0	3.12646958082465	3.9920701518503644E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8945	0.0	66.91247	1
GTATCAA	18465	0.0	46.546375	1
TATAACG	240	0.0	39.181202	2
GTGGTAT	2610	0.0	38.910988	1
TGGTATC	2540	0.0	38.87269	2
ATCAACG	24430	0.0	35.063713	3
TATCAAC	24450	0.0	35.03713	2
TCAACGC	24660	0.0	34.698547	4
CAACGCA	25400	0.0	33.650627	5
AACGCAG	26350	0.0	32.514942	6
CGCAGAG	29480	0.0	28.871405	8
ACGCAGA	29605	0.0	28.797127	7
GCAGAGT	30910	0.0	27.338055	9
GTACATG	15535	0.0	27.148148	1
CATGGGT	6680	0.0	26.604084	4
TACATGG	15495	0.0	26.489985	2
ACATGGG	15505	0.0	25.561644	3
ATAACGC	410	0.0	25.227362	3
GAGTACT	22500	0.0	23.66597	12-13
TACCTGG	1730	0.0	23.644611	2
>>END_MODULE