FastQCFastQC Report
Wed 25 May 2016
SRR2431395_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431395_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3869846
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA207930.5373082029620817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57700.048.123261
GTATCAA94050.036.879711
TCAACGC108050.030.9191884
ATCAACG109200.030.5528563
TATCAAC119050.028.736462
CAACGCA117650.028.3962445
AACGCAG123800.026.9131036
CATGGGT76150.026.5082994
GTACATG199050.025.4879781
TACATGG197200.024.578682
CGCAGAG135650.024.215938
ACGCAGA136900.024.0294577
ATGGGTG46200.023.8324325
ACATGGG199800.023.5287153
GTGGTAT27100.022.749971
TGGTATC27000.021.4372752
TACCTGG27850.021.2898962
GCAGAGT155050.020.5497889
CAGAGTA149150.018.7159810-11
GTACCTG32550.018.6516631
Produced by FastQC (version 0.11.5)