##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431395_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3869846
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.761725660400955	33.0	31.0	34.0	27.0	34.0
2	30.883670564668463	33.0	31.0	34.0	27.0	34.0
3	31.009651030040988	34.0	31.0	34.0	27.0	34.0
4	34.25079059993602	37.0	35.0	37.0	30.0	37.0
5	34.37725919842805	37.0	35.0	37.0	31.0	37.0
6	34.40090535902462	37.0	35.0	37.0	31.0	37.0
7	34.33835015656954	37.0	35.0	37.0	31.0	37.0
8	34.27478587003204	37.0	35.0	37.0	31.0	37.0
9	35.765482399041204	39.0	37.0	39.0	30.0	39.0
10-11	35.78134297850612	39.0	37.0	39.0	30.0	39.0
12-13	35.710634738436625	39.0	37.0	39.0	30.0	39.0
14-15	37.07531643378057	41.0	38.0	41.0	30.5	41.0
16-17	37.09620266542906	41.0	38.0	41.0	30.5	41.0
18-19	37.123991884948396	41.0	38.0	41.0	30.0	41.0
20-21	37.193173190871164	41.0	38.0	41.0	30.0	41.0
22-23	37.16810294259771	41.0	38.0	41.0	30.0	41.0
24-25	37.20542406597059	41.0	38.0	41.0	30.0	41.0
26-27	37.23252566122787	41.0	38.0	41.0	30.0	41.0
28-29	37.19599113246367	40.0	38.0	41.0	30.0	41.0
30-31	37.127664640918525	40.0	38.0	41.0	30.0	41.0
32-33	37.07237523146916	40.0	38.0	41.0	30.0	41.0
34-35	36.99875111309339	40.0	38.0	41.0	30.0	41.0
36-37	36.87507745786266	40.0	38.0	41.0	30.0	41.0
38-39	36.75540344499497	40.0	37.5	41.0	30.0	41.0
40-41	36.687996628289596	40.0	37.0	41.0	29.5	41.0
42-43	36.53451881547741	40.0	37.0	41.0	29.0	41.0
44-45	36.39438636576236	40.0	37.0	41.0	29.0	41.0
46-47	36.270513865409626	40.0	36.0	41.0	28.5	41.0
48-49	36.13469386120275	40.0	36.0	41.0	28.0	41.0
50-51	36.00169257381302	40.0	36.0	41.0	28.0	41.0
52-53	35.83887420843104	40.0	35.0	41.0	28.0	41.0
54-55	35.675392767567494	40.0	35.0	41.0	28.0	41.0
56-57	35.48139021552795	39.0	35.0	41.0	28.0	41.0
58-59	35.26564790950337	39.0	35.0	41.0	27.0	41.0
60-61	35.02765665610467	39.0	34.5	41.0	26.5	41.0
62-63	34.91728753547299	38.5	34.5	41.0	26.5	41.0
64-65	34.66047344002836	38.0	34.0	40.5	26.0	41.0
66-67	34.383266285014955	37.5	34.0	40.0	26.0	41.0
68-69	34.074915771842086	37.0	34.0	40.0	26.0	41.0
70-71	33.73512602310272	36.5	34.0	39.5	25.0	41.0
72-73	33.38425921858389	36.0	33.0	39.0	24.0	41.0
74-75	33.0897379378921	36.0	33.0	39.0	24.0	41.0
76-77	32.82430179392152	35.0	33.0	38.5	23.5	40.0
78-79	32.476997017452376	35.0	33.0	37.0	23.0	39.5
80-81	32.15374358566207	35.0	33.0	37.0	22.0	39.0
82-83	31.83379752062485	35.0	33.0	36.5	21.0	39.0
84-85	31.54237778454233	35.0	33.0	36.0	20.0	37.5
86-87	31.27026398466502	35.0	32.0	36.0	20.0	37.0
88-89	31.01185602734579	35.0	32.0	35.5	18.5	37.0
90-91	30.787510148982673	35.0	32.0	35.0	17.5	36.0
92-93	30.564621822160365	35.0	32.0	35.0	10.0	36.0
94-95	30.358661171529825	35.0	32.0	35.0	4.5	36.0
96-97	30.167720756846656	35.0	31.5	35.0	2.0	36.0
98-99	29.95632539382704	35.0	31.0	35.0	2.0	35.5
100	29.788781775812268	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.23696488504160484
1101	2	0.2568057990681858
1101	3	0.2525949055472765
1101	4	0.2791672950792332
1101	5	0.24923012497744423
1101	6	0.2241359788517201
1101	7	0.17748847904281462
1101	8	0.21363433696324563
1101	9	0.21072205820345857
1101	10-11	0.2253856228831097
1101	12-13	0.17167214752693383
1101	14-15	0.25771019424617236
1101	16-17	0.1920543879703871
1101	18-19	0.16000561311220451
1101	20-21	0.19608093868546916
1101	22-23	0.21634941502524896
1101	24-25	0.24135634784088467
1101	26-27	0.24563842822995952
1101	28-29	0.22384118155500943
1101	30-31	0.2642336690951339
1101	32-33	0.23461064738694404
1101	34-35	0.18272594842147072
1101	36-37	0.20678289737337252
1101	38-39	0.24581895311712998
1101	40-41	0.21369836886875504
1101	42-43	0.25195287075590045
1101	44-45	0.23652772020626855
1101	46-47	0.17849849777219617
1101	48-49	0.22834226406758518
1101	50-51	0.2522998346590768
1101	52-53	0.16942489601927235
1101	54-55	0.20328471861783015
1101	56-57	0.13476494533549754
1101	58-59	0.16608135434655935
1101	60-61	0.1879779211182253
1101	62-63	0.2590954008928392
1101	64-65	0.20647998885782215
1101	66-67	0.18114919096273496
1101	68-69	0.22335820971916576
1101	70-71	0.2065169600512604
1101	72-73	0.2412382881936992
1101	74-75	0.24056956228692172
1101	76-77	0.20161405544915567
1101	78-79	0.20672637976359454
1101	80-81	0.18217077716280983
1101	82-83	0.1755062947901216
1101	84-85	0.13786365625745134
1101	86-87	0.07643004977775547
1101	88-89	0.12163794459770827
1101	90-91	0.12089959321552612
1101	92-93	0.10818469463537284
1101	94-95	0.13832211531464367
1101	96-97	0.1396785491035608
1101	98-99	0.13227610007744772
1101	100	0.12181109937108303
1102	1	0.005514916063429354
1102	2	0.015655492065725696
1102	3	0.06945605396203192
1102	4	0.04784858956679727
1102	5	0.0988711948189831
1102	6	0.07808069311907673
1102	7	0.045016234044723547
1102	8	0.10286626532175802
1102	9	0.06720686495475547
1102	10-11	0.10133728300971256
1102	12-13	0.0927302350445558
1102	14-15	0.08709872344962832
1102	16-17	0.0352920793406426
1102	18-19	-0.016174991038653275
1102	20-21	0.01670347540939332
1102	22-23	0.06231276498186844
1102	24-25	0.14174162697376858
1102	26-27	0.09038080329890619
1102	28-29	1.0286095455569466E-4
1102	30-31	0.08510072815290926
1102	32-33	0.0782482590619864
1102	34-35	0.10477984102831073
1102	36-37	0.07956594088081914
1102	38-39	0.06868429785478725
1102	40-41	0.057480755481194024
1102	42-43	0.003258797818084247
1102	44-45	-0.04792361198632733
1102	46-47	-0.07615962219863093
1102	48-49	-0.08937232153076735
1102	50-51	-0.1370462832865016
1102	52-53	-0.08745834830523336
1102	54-55	-0.1643111714299863
1102	56-57	-0.20140181741987817
1102	58-59	-0.143863204330259
1102	60-61	-0.120034272455392
1102	62-63	-0.07750680995621195
1102	64-65	-0.14776653509946414
1102	66-67	-0.10097505501049397
1102	68-69	-0.13921016121584984
1102	70-71	-0.09378573542636559
1102	72-73	-0.1555533566973537
1102	74-75	-0.10691150143369299
1102	76-77	-0.06613064693067372
1102	78-79	-0.07090474024304427
1102	80-81	-0.10964347264497576
1102	82-83	-0.12971207305747967
1102	84-85	-0.14147122502196652
1102	86-87	-0.18201474838268084
1102	88-89	-0.17534856716651603
1102	90-91	-0.18543518805974202
1102	92-93	-0.20201927787850948
1102	94-95	-0.17645207236990146
1102	96-97	-0.19773316257588291
1102	98-99	-0.28476192345035756
1102	100	-0.32308038971119757
1103	1	-0.3736722702311468
1103	2	-0.37756407977492756
1103	3	-0.24169581492852288
1103	4	-0.2926677957360866
1103	5	-0.26092789860190635
1103	6	-0.2569232588092589
1103	7	-0.28190349114426283
1103	8	-0.29919684393385637
1103	9	-0.2553204776605469
1103	10-11	-0.27050066766211955
1103	12-13	-0.2999674795924392
1103	14-15	-0.3088449767712689
1103	16-17	-0.3440582506622647
1103	18-19	-0.3399071651396639
1103	20-21	-0.4123864326491642
1103	22-23	-0.336158818288979
1103	24-25	-0.24784352188219572
1103	26-27	-0.2851567602866041
1103	28-29	-0.3601900546227981
1103	30-31	-0.2965754974515278
1103	32-33	-0.2547037300752777
1103	34-35	-0.31030436544474327
1103	36-37	-0.39330153778890065
1103	38-39	-0.3287618106779959
1103	40-41	-0.2565462257184379
1103	42-43	-0.30131159041665256
1103	44-45	-0.39374436332558815
1103	46-47	-0.49684374490431793
1103	48-49	-0.44605580922574717
1103	50-51	-0.4571220792454014
1103	52-53	-0.5405628075665945
1103	54-55	-0.5173446986442443
1103	56-57	-0.5409143453704814
1103	58-59	-0.5448900301549315
1103	60-61	-0.5305254599929654
1103	62-63	-0.45374423640643613
1103	64-65	-0.5033880687178822
1103	66-67	-0.4955399713444564
1103	68-69	-0.5137938576537096
1103	70-71	-0.47674042673685335
1103	72-73	-0.5168768135875581
1103	74-75	-0.5501564061483109
1103	76-77	-0.47507990687298474
1103	78-79	-0.4800320058464038
1103	80-81	-0.4510397531035366
1103	82-83	-0.42253172326115873
1103	84-85	-0.4804260919737118
1103	86-87	-0.46735376979064824
1103	88-89	-0.5044948830910982
1103	90-91	-0.4838304349370439
1103	92-93	-0.5304295893715256
1103	94-95	-0.6124017849262238
1103	96-97	-0.589035204743297
1103	98-99	-0.7136478174305942
1103	100	-0.6291967588010365
1104	1	-0.3573345238951866
1104	2	-0.35519031706428095
1104	3	-0.33752391661937153
1104	4	-0.2391188289795707
1104	5	-0.2867810460430533
1104	6	-0.2602378763254123
1104	7	-0.34824572511936935
1104	8	-0.31984045641723924
1104	9	-0.3532130823146531
1104	10-11	-0.4213799615061191
1104	12-13	-0.37976710414238113
1104	14-15	-0.43175191825150705
1104	16-17	-0.3701236870544662
1104	18-19	-0.4477437168188487
1104	20-21	-0.48470072233146766
1104	22-23	-0.4036435107156464
1104	24-25	-0.46126638125470265
1104	26-27	-0.4107417830979827
1104	28-29	-0.39267409043699786
1104	30-31	-0.3379720318603887
1104	32-33	-0.3524985041958715
1104	34-35	-0.322971789813856
1104	36-37	-0.39532763600867327
1104	38-39	-0.3647816337610905
1104	40-41	-0.3889313917563655
1104	42-43	-0.47838036844667897
1104	44-45	-0.44803629417248914
1104	46-47	-0.5432547673018888
1104	48-49	-0.6035993416874419
1104	50-51	-0.6373248827444442
1104	52-53	-0.7102976495025004
1104	54-55	-0.5393984690641886
1104	56-57	-0.5988033315702808
1104	58-59	-0.6289773341041496
1104	60-61	-0.626059550461882
1104	62-63	-0.5649773640641769
1104	64-65	-0.5507294968074774
1104	66-67	-0.4820363344672103
1104	68-69	-0.5122553059990906
1104	70-71	-0.5913894036023635
1104	72-73	-0.577750504968364
1104	74-75	-0.5104762756194177
1104	76-77	-0.5087698421848472
1104	78-79	-0.5080578792118402
1104	80-81	-0.4895239986558124
1104	82-83	-0.5668170218497721
1104	84-85	-0.5071300957981251
1104	86-87	-0.44035268425435703
1104	88-89	-0.5200491348693852
1104	90-91	-0.5632008994691056
1104	92-93	-0.6140007675799275
1104	94-95	-0.5319824410285179
1104	96-97	-0.4773928043948672
1104	98-99	-0.5594852723062012
1104	100	-0.5840461961455645
1105	1	-0.06325033355635057
1105	2	-0.08628771167689209
1105	3	0.003163719040241375
1105	4	0.12945859150277528
1105	5	0.11146540816980632
1105	6	0.10030394986782909
1105	7	0.11163400681839875
1105	8	0.07128759327088119
1105	9	0.11002900494045775
1105	10-11	0.08071374633810535
1105	12-13	0.13235872280161942
1105	14-15	0.06998873922157145
1105	16-17	0.07091901430676018
1105	18-19	0.1093926480370726
1105	20-21	0.09846731102329187
1105	22-23	-5.462663033384274E-4
1105	24-25	-0.08668646127251378
1105	26-27	-0.01115004984883683
1105	28-29	0.02540695231091661
1105	30-31	0.08204930246481013
1105	32-33	0.06702493936563059
1105	34-35	-0.02119317184541103
1105	36-37	3.147845784212677E-4
1105	38-39	0.015386356591790218
1105	40-41	-0.07289173789335024
1105	42-43	-0.04535430256656525
1105	44-45	-0.180461540419536
1105	46-47	-0.057584103910230056
1105	48-49	-0.14374199890740869
1105	50-51	-0.09110692297681311
1105	52-53	-0.05498902399830996
1105	54-55	-0.14039433635032594
1105	56-57	-0.0921479630055444
1105	58-59	-0.07366095355928337
1105	60-61	-0.16216364467006628
1105	62-63	-0.05175379457209317
1105	64-65	-0.03151013537443248
1105	66-67	-0.06684603520226773
1105	68-69	-0.03398617349901656
1105	70-71	0.05825840973956531
1105	72-73	0.003890103904012676
1105	74-75	-0.007599957982264982
1105	76-77	-0.006563912898805313
1105	78-79	0.03567537409632848
1105	80-81	0.0018423623299383962
1105	82-83	0.09946706893541801
1105	84-85	-0.01366791362768538
1105	86-87	0.025817053371667953
1105	88-89	0.05664322511691111
1105	90-91	-0.04282805230454301
1105	92-93	-0.038335582946867675
1105	94-95	-0.12267317012852885
1105	96-97	-0.07494444739150907
1105	98-99	-0.11760841402142219
1105	100	-0.10135378078362578
1106	1	-0.2801781700352777
1106	2	-0.20620908478994693
1106	3	-0.20790322770077196
1106	4	-0.10026841346710569
1106	5	-0.18124050360000155
1106	6	-0.13396728396649138
1106	7	-0.08285631386640802
1106	8	-0.18107196370946355
1106	9	-0.12566605983843715
1106	10-11	-0.12206367709733001
1106	12-13	-0.12446630423598037
1106	14-15	-0.13750413708515907
1106	16-17	-0.1559048980652733
1106	18-19	-0.22565663688258297
1106	20-21	-0.27229027305952513
1106	22-23	-0.20565214258781594
1106	24-25	-0.19273884111374429
1106	26-27	-0.21511036823896035
1106	28-29	-0.18054436330911017
1106	30-31	-0.1564088258071763
1106	32-33	-0.23524694511386457
1106	34-35	-0.15960822029040145
1106	36-37	-0.17777207050102106
1106	38-39	-0.15278926386906733
1106	40-41	-0.18883051140783635
1106	42-43	-0.19196610654809376
1106	44-45	-0.2528926841027044
1106	46-47	-0.32593820399146267
1106	48-49	-0.22069746239044008
1106	50-51	-0.308938655193721
1106	52-53	-0.36334131229415334
1106	54-55	-0.3469305488208292
1106	56-57	-0.37639875525317734
1106	58-59	-0.4306845810804063
1106	60-61	-0.44637349928185444
1106	62-63	-0.3909537261188021
1106	64-65	-0.3122737853679922
1106	66-67	-0.23688049059192906
1106	68-69	-0.25181427488347197
1106	70-71	-0.3508581627352356
1106	72-73	-0.36501321404749376
1106	74-75	-0.2833394133186715
1106	76-77	-0.1941645474714946
1106	78-79	-0.18356950836636088
1106	80-81	-0.21320948353687186
1106	82-83	-0.21910201878646518
1106	84-85	-0.2535447594218745
1106	86-87	-0.23791342364225798
1106	88-89	-0.22824514130863705
1106	90-91	-0.26983026880672156
1106	92-93	-0.2336885591644311
1106	94-95	-0.29991510024032664
1106	96-97	-0.29141131943419296
1106	98-99	-0.2882080387385777
1106	100	-0.3048345872350815
1107	1	-0.4944572447343276
1107	2	-0.48813653913778055
1107	3	-0.4021291544614094
1107	4	-0.3851881732981397
1107	5	-0.3045039120669628
1107	6	-0.4440428074817646
1107	7	-0.41806400591076454
1107	8	-0.47573468017866816
1107	9	-0.4940414080006974
1107	10-11	-0.40848921791967996
1107	12-13	-0.4526936638228136
1107	14-15	-0.5058678956875866
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2216	64-65	0.2630784835977309
2216	66-67	0.17285012912112308
2216	68-69	0.1949274524826876
2216	70-71	0.09806150089526966
2216	72-73	0.1562792756062663
2216	74-75	0.16662665355927686
2216	76-77	0.23669008414228898
2216	78-79	0.275217932894094
2216	80-81	0.2515229357799029
2216	82-83	0.2879130206741465
2216	84-85	0.261593837782069
2216	86-87	0.2316293225886703
2216	88-89	0.26259855805894716
2216	90-91	0.24443673814144873
2216	92-93	0.28995757063827554
2216	94-95	0.2755260105487416
2216	96-97	0.26082594040086704
2216	98-99	0.24922688648184632
2216	100	0.23304697231061766
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	91609.0
3	18202.0
4	13285.0
5	11510.0
6	7673.0
7	6416.0
8	7005.0
9	7263.0
10	7400.0
11	8000.0
12	9315.0
13	10670.0
14	11577.0
15	12218.0
16	12541.0
17	12012.0
18	11839.0
19	10950.0
20	10537.0
21	11076.0
22	13070.0
23	18808.0
24	29706.0
25	38739.0
26	42272.0
27	33073.0
28	33476.0
29	41400.0
30	49374.0
31	56861.0
32	72340.0
33	97730.0
34	138415.0
35	217578.0
36	371552.0
37	690767.0
38	1069524.0
39	561526.0
40	2537.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.8605560268691	21.782435051713467	14.219410276729368	22.137598644688065
2	14.916278229336713	22.55833290619465	39.91069953925974	22.614689325208897
3	17.343048563984624	28.74179685163768	30.485613955507844	23.42954062886985
4	11.7826356417363	19.325947645041687	39.0102635765999	29.881153136622117
5	12.056177340314722	40.55709221703615	34.829914747004935	12.556815695644191
6	27.69855095251494	40.29832296132012	18.75328435591453	13.249841730250417
7	24.81241371962116	35.60991984491385	23.01054062399625	16.567125811468742
8	23.881899201102605	37.41604078397705	21.172681562006666	17.52937845291367
9	24.817151719469393	18.87716184509856	21.50568829714119	34.79999813829085
10-11	22.340303657926974	29.54579837115718	28.989618861744383	19.124279109171464
12-13	23.06397212795848	27.747214703032036	28.527130179217124	20.66168298979236
14-15	21.04016931798155	29.170191646408945	26.066657900279633	23.72298113532987
16-17	20.219528936970892	32.18445850662348	26.84263339047647	20.753379165929157
18-19	20.3019625275698	31.10887165073314	28.576070409048043	20.013095412649022
20-21	21.953614738477288	30.027687270514576	27.921564327551547	20.097133663456592
22-23	21.656506137240783	30.110564851649464	27.823472755993556	20.409456255116197
24-25	21.18766002222371	30.1834787890793	27.84820486775289	20.780656320944104
26-27	20.90637779507842	30.15501918392949	27.99002096894716	20.948582052044934
28-29	20.895027575732737	30.059669194646894	28.205059908398965	20.840243321221404
30-31	21.072092883424396	30.29887251222431	27.92390055556525	20.70513404878604
32-33	20.864663404098174	30.224286067467105	28.10726288709382	20.8037876413409
34-35	21.158205374906885	29.95377320956365	28.06088111358064	20.82714030194883
36-37	21.219469899261384	30.007618017523118	27.779629154751795	20.9932829284637
38-39	21.031696976060587	30.098368677195257	27.975797312318225	20.894137034425935
40-41	21.046853036447143	29.881726621421357	28.127609927107272	20.943810415024235
42-43	20.997161544468426	29.91537649977093	28.11095101255833	20.97651094320231
44-45	21.023906453682052	29.993495106457818	28.022615820728753	20.95998261913137
46-47	21.120789758300486	30.003475830307067	27.980938277632983	20.894796133759467
48-49	21.033985606379098	29.871606253141564	27.938490696183077	21.155917444296264
50-51	20.960451867814697	29.908712201297387	27.943635490192655	21.187200440695257
52-53	21.019123023352527	29.914747864300033	27.97395832255742	21.092170789790018
54-55	21.0816506855701	30.052970316682654	27.897626842397305	20.967752155349938
56-57	21.055798848366138	29.912766295288108	27.791157876661494	21.24027697968426
58-59	21.1356539080704	29.697432327705542	27.93949862105985	21.227415143164208
60-61	21.179943848527575	29.99308968614968	27.757931602375574	21.069034862947166
62-63	20.995823775452322	29.910167016657184	27.915744711772756	21.178264496117734
64-65	21.14659126408626	29.771045864271215	27.93014692952059	21.152215942121934
66-67	20.95589785019508	29.87087360990393	27.847190094207026	21.326038445693964
68-69	21.23500158587497	29.944707076028	27.75323863080223	21.067052707294806
70-71	21.216520008704187	29.77665847923168	27.814984053314305	21.19183745874982
72-73	21.136937487418265	29.87225815176742	27.928959701862855	21.061844658951465
74-75	21.12514232561411	29.925461848718893	27.831222703414753	21.11817312225224
76-77	21.215167325238752	29.840963860406937	27.66619543602368	21.277673378330622
78-79	21.23354402923753	29.845905588322573	27.837822985701255	21.082727396738644
80-81	21.039357502008194	29.80890733030953	27.91803671284091	21.233698454841367
82-83	21.102203913853483	29.78883119103263	28.016355977999787	21.0926089171141
84-85	21.10906118313015	29.753242667232893	27.820244061117826	21.31745208851913
86-87	21.296769236379305	29.80199246328431	27.757197341071944	21.144040959264444
88-89	21.167443833228592	29.692392043676502	27.846821893079465	21.29334223001544
90-91	21.09908241695718	29.71535011948235	27.780951617408217	21.404615846152257
92-93	21.155612814535598	29.741567851611205	27.800336718316547	21.30248261553665
94-95	21.066699835805007	29.64722319877809	27.933008856754704	21.353068108662203
96-97	21.1309295999007	29.784460465886053	27.727279779883325	21.357330154329926
98-99	21.05255467354665	29.707050858644035	27.8110046918828	21.42938977592652
100	21.07587665278451	29.793935061300648	27.700283005643044	21.429905280271804
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19680.0
1	14777.5
2	8737.5
3	6426.5
4	4425.0
5	3243.5
6	2718.0
7	2547.0
8	2675.5
9	2674.5
10	2230.0
11	1854.5
12	1739.0
13	1716.5
14	1814.5
15	1927.0
16	2170.0
17	2650.5
18	3339.5
19	4425.5
20	5956.5
21	8358.0
22	12271.5
23	17755.0
24	24140.5
25	30899.5
26	39605.5
27	50107.0
28	63331.0
29	76439.0
30	84338.5
31	92410.5
32	101042.5
33	107939.0
34	115040.0
35	121029.0
36	123848.5
37	125808.0
38	126982.5
39	127335.0
40	128326.5
41	127476.5
42	124865.0
43	122566.0
44	121620.0
45	120409.0
46	118649.0
47	116445.0
48	112987.0
49	110358.0
50	109016.0
51	104820.0
52	98525.0
53	92495.0
54	88418.0
55	86402.5
56	79762.5
57	70318.0
58	63480.5
59	58192.5
60	51309.0
61	43262.0
62	37277.0
63	31214.0
64	24770.0
65	20059.0
66	16361.5
67	12841.5
68	9868.0
69	7484.5
70	5533.5
71	4265.5
72	3349.0
73	2483.5
74	1926.0
75	1473.0
76	1033.5
77	808.0
78	629.0
79	444.5
80	336.0
81	243.0
82	172.5
83	131.5
84	113.5
85	110.0
86	86.5
87	65.5
88	62.5
89	63.5
90	56.0
91	40.0
92	33.0
93	29.0
94	24.0
95	23.0
96	22.5
97	26.0
98	27.0
99	23.0
100	37.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18396080877636992
2	0.17912857514226663
3	0.19269500646795765
4	0.18243620030357796
5	0.195795905056687
6	0.0782976893654166
7	0.07989982030292679
8	0.06824560977361889
9	0.06284487806491525
10-11	0.07568776638656939
12-13	0.07464121311287322
14-15	0.07241890245761717
16-17	0.06864614250799644
18-19	0.07346545573131334
20-21	0.07510634790118263
22-23	0.07337501285580873
24-25	0.07248350451154904
26-27	0.07590741336993771
28-29	0.07387890887647726
30-31	0.07916335688810357
32-33	0.076333786925888
34-35	0.075778209262074
36-37	0.08668303596577229
38-39	0.08186372274245539
40-41	0.0775095443074479
42-43	0.08089469193347745
44-45	0.09111473686549801
46-47	0.08124354302470951
48-49	0.0863471052853266
50-51	0.08342709244760645
52-53	0.08263894738963773
54-55	0.08389222723591586
56-57	0.0773157381456523
58-59	0.08500338256354388
60-61	0.08170867781301891
62-63	0.0732974903910905
64-65	0.07643715021217899
66-67	0.07589449295915135
68-69	0.07482209886388244
70-71	0.07084261234167974
72-73	0.06802596279025057
74-75	0.07320704751558589
76-77	0.07805220156047553
78-79	0.07489962132860067
80-81	0.08238053917391028
82-83	0.0839180680574886
84-85	0.0856494031028625
86-87	0.0820446084934646
88-89	0.08362089860940203
90-91	0.0818120410993099
92-93	0.07275483313806286
94-95	0.07228969834975345
96-97	0.07250934533312178
98-99	0.08186372274245539
100	0.09023614893202468
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3869846.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.39538986128721
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.94607870704654	44.51451563379745
2	15.00762434143324	17.82767397302342
3	4.649073128662406	8.284005329116136
4	2.015803867729457	4.789178264307269
5	0.9972313163765073	2.961547140903365
6	0.5607638518657951	1.9984072561011608
7	0.3706195600902241	1.5409165283244337
8	0.25058758370221773	1.190699778271293
9	0.18549368933164026	0.9915722995195139
>10	0.9325978692202261	9.755835395322409
>50	0.055635485504645206	2.2943911447324044
>100	0.02718460612078426	2.6868546686760966
>500	0.0010447943531764047	0.411431059438659
>1k	2.1766546930855382E-4	0.21533418801099802
>5k	0.0	0.0
>10k+	4.353309386171067E-5	0.5376373404554124
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20793	0.5373082029620817	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	2.5840821572744755E-5
7	0.0	0.0	0.0	0.0	2.5840821572744755E-5
8	0.0	0.0	0.0	0.0	2.5840821572744755E-5
9	0.0	0.0	0.0	2.5840821572744755E-5	2.5840821572744755E-5
10-11	0.0	0.0	0.0	2.5840821572744755E-5	2.5840821572744755E-5
12-13	0.0	0.0	0.0	3.876123235911713E-5	2.5840821572744755E-5
14-15	0.0	0.0	0.0	7.752246471823427E-5	2.5840821572744755E-5
16-17	0.0	0.0	0.0	1.0336328629097902E-4	2.5840821572744755E-5
18-19	0.0	0.0	0.0	1.0336328629097902E-4	2.5840821572744755E-5
20-21	0.0	0.0	0.0	1.0336328629097902E-4	2.5840821572744755E-5
22-23	0.0	2.5840821572744755E-5	0.0	2.0672657258195804E-4	2.5840821572744755E-5
24-25	0.0	2.5840821572744755E-5	0.0	4.522143775230332E-4	2.5840821572744755E-5
26-27	0.0	2.5840821572744755E-5	0.0	9.431899874051835E-4	2.5840821572744755E-5
28-29	0.0	2.5840821572744755E-5	0.0	0.002429037227838007	2.5840821572744755E-5
30-31	0.0	2.5840821572744755E-5	0.0	0.0060079910156631555	2.5840821572744755E-5
32-33	0.0	2.5840821572744755E-5	0.0	0.012442355587276601	2.5840821572744755E-5
34-35	0.0	2.5840821572744755E-5	0.0	0.022546116822219797	2.5840821572744755E-5
36-37	2.5840821572744755E-5	2.5840821572744755E-5	0.0	0.04013079590247261	2.5840821572744755E-5
38-39	2.5840821572744755E-5	2.5840821572744755E-5	0.0	0.06367178435524307	2.5840821572744755E-5
40-41	2.5840821572744755E-5	2.5840821572744755E-5	0.0	0.08889242621024196	2.5840821572744755E-5
42-43	2.5840821572744755E-5	2.5840821572744755E-5	0.0	0.11319571889940841	2.5840821572744755E-5
44-45	2.5840821572744755E-5	5.168164314548951E-5	0.0	0.14306770863750135	2.5840821572744755E-5
46-47	2.5840821572744755E-5	5.168164314548951E-5	0.0	0.18339231070176953	2.5840821572744755E-5
48-49	2.5840821572744755E-5	5.168164314548951E-5	0.0	0.22608134793994386	2.5840821572744755E-5
50-51	2.5840821572744755E-5	5.168164314548951E-5	0.0	0.2698815405057462	2.5840821572744755E-5
52-53	2.5840821572744755E-5	5.168164314548951E-5	0.0	0.31369465348233494	5.168164314548951E-5
54-55	2.5840821572744755E-5	5.168164314548951E-5	0.0	0.3630506226862774	5.168164314548951E-5
56-57	2.5840821572744755E-5	5.168164314548951E-5	0.0	0.4239445187224504	5.168164314548951E-5
58-59	2.5840821572744755E-5	5.168164314548951E-5	0.0	0.49302995519718357	5.168164314548951E-5
60-61	2.5840821572744755E-5	5.168164314548951E-5	0.0	0.5665987742147878	5.168164314548951E-5
62-63	2.5840821572744755E-5	5.168164314548951E-5	0.0	0.6440953981114494	5.168164314548951E-5
64-65	2.5840821572744755E-5	5.168164314548951E-5	0.0	0.7249384084017814	5.168164314548951E-5
66-67	2.5840821572744755E-5	5.168164314548951E-5	0.0	0.8220223750505834	5.168164314548951E-5
68-69	2.5840821572744755E-5	5.168164314548951E-5	0.0	0.9357995124353786	5.168164314548951E-5
70-71	2.5840821572744755E-5	5.168164314548951E-5	0.0	1.0624324585526144	5.168164314548951E-5
72-73	2.5840821572744755E-5	5.168164314548951E-5	0.0	1.1940526832333895	5.168164314548951E-5
74-75	2.5840821572744755E-5	5.168164314548951E-5	0.0	1.331086560033655	5.168164314548951E-5
76-77	2.5840821572744755E-5	5.168164314548951E-5	0.0	1.4823070478773575	5.168164314548951E-5
78-79	2.5840821572744755E-5	5.168164314548951E-5	0.0	1.6576499426592168	5.168164314548951E-5
80-81	2.5840821572744755E-5	5.168164314548951E-5	0.0	1.8555131134417235	5.168164314548951E-5
82-83	2.5840821572744755E-5	5.168164314548951E-5	0.0	2.0688936975786634	5.168164314548951E-5
84-85	2.5840821572744755E-5	5.168164314548951E-5	0.0	2.2850521700346733	5.168164314548951E-5
86-87	2.5840821572744755E-5	5.168164314548951E-5	0.0	2.51568150257142	5.168164314548951E-5
88	2.5840821572744755E-5	5.168164314548951E-5	0.0	2.7047329531976207	5.168164314548951E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5770	0.0	48.12326	1
GTATCAA	9405	0.0	36.87971	1
TCAACGC	10805	0.0	30.919188	4
ATCAACG	10920	0.0	30.552856	3
TATCAAC	11905	0.0	28.73646	2
CAACGCA	11765	0.0	28.396244	5
AACGCAG	12380	0.0	26.913103	6
CATGGGT	7615	0.0	26.508299	4
GTACATG	19905	0.0	25.487978	1
TACATGG	19720	0.0	24.57868	2
CGCAGAG	13565	0.0	24.21593	8
ACGCAGA	13690	0.0	24.029457	7
ATGGGTG	4620	0.0	23.832432	5
ACATGGG	19980	0.0	23.528715	3
GTGGTAT	2710	0.0	22.74997	1
TGGTATC	2700	0.0	21.437275	2
TACCTGG	2785	0.0	21.289896	2
GCAGAGT	15505	0.0	20.549788	9
CAGAGTA	14915	0.0	18.71598	10-11
GTACCTG	3255	0.0	18.651663	1
>>END_MODULE