FastQCFastQC Report
Wed 25 May 2016
SRR2431395_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431395_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3869846
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319170.8247615021372943No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259130.6696132094145348No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168500.43541784350074914No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58100.150135173337647No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54030.13961795895753992No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46720.12072831838786349No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40080.10357001286356099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103300.066.1838151
GTATCAA203700.045.450891
GTGGTAT30450.036.442861
ATCAACG263800.034.913333
TCAACGC266100.034.6468964
TATCAAC266700.034.5535552
CAACGCA276550.033.3206945
TGGTATC31750.033.3194962
AACGCAG284150.032.5504886
ACGCAGA321200.028.7226727
CGCAGAG320950.028.6571858
GCAGAGT348000.026.1734039
GTACATG183250.025.7362141
CATGGGT74450.025.3859214
TACATGG182950.025.1342582
ACATGGG183700.024.2878283
GAGTACT241850.023.43618412-13
CAGAGTA343200.022.66399210-11
AGAGTAC329350.022.06875810-11
AGTACTT248050.021.33461612-13
Produced by FastQC (version 0.11.5)