##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431395_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3869846 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66721750684653 34.0 31.0 34.0 31.0 34.0 2 32.79493835155198 34.0 31.0 34.0 31.0 34.0 3 32.87337428931281 34.0 31.0 34.0 31.0 34.0 4 36.33449134668408 37.0 37.0 37.0 35.0 37.0 5 36.24096462753298 37.0 37.0 37.0 35.0 37.0 6 36.28247584012387 37.0 37.0 37.0 35.0 37.0 7 36.24071164588978 37.0 37.0 37.0 35.0 37.0 8 36.25864750173521 37.0 37.0 37.0 35.0 37.0 9 38.060405762916666 39.0 39.0 39.0 35.0 39.0 10-11 38.0370128423715 39.0 39.0 39.0 35.0 39.0 12-13 38.01766581925999 39.0 39.0 39.0 35.0 39.0 14-15 39.49297426822669 41.0 39.0 41.0 36.0 41.0 16-17 39.520477042238895 41.0 39.0 41.0 36.5 41.0 18-19 39.588791647006104 41.0 40.0 41.0 37.0 41.0 20-21 39.585877448353244 41.0 40.0 41.0 37.0 41.0 22-23 39.52913979005883 41.0 40.0 41.0 36.0 41.0 24-25 39.47922527149659 41.0 39.5 41.0 36.0 41.0 26-27 39.36302878719205 41.0 39.0 41.0 36.0 41.0 28-29 39.28557570507974 41.0 39.0 41.0 36.0 41.0 30-31 39.11315695766705 41.0 39.0 41.0 35.0 41.0 32-33 38.899304907740515 41.0 38.0 41.0 35.0 41.0 34-35 38.77475666990366 41.0 38.0 41.0 35.0 41.0 36-37 38.643645250999654 41.0 38.0 41.0 35.0 41.0 38-39 38.50690027458457 41.0 38.0 41.0 35.0 41.0 40-41 38.37450831376752 41.0 38.0 41.0 35.0 41.0 42-43 38.214238499413156 40.0 38.0 41.0 34.0 41.0 44-45 37.98377209325642 40.0 38.0 41.0 33.5 41.0 46-47 37.63831325587633 40.0 38.0 41.0 33.0 41.0 48-49 37.320814058233836 40.0 37.5 41.0 32.5 41.0 50-51 37.152520539577026 40.0 37.0 41.0 32.0 41.0 52-53 37.087474281922326 40.0 37.0 41.0 32.0 41.0 54-55 36.9419646156462 40.0 36.5 41.0 32.0 41.0 56-57 36.78259948845509 40.0 36.0 41.0 31.5 41.0 58-59 36.59742648673875 40.0 35.5 41.0 31.0 41.0 60-61 36.43734040062576 39.5 35.0 41.0 31.0 41.0 62-63 36.272677775808134 39.0 35.0 41.0 31.0 41.0 64-65 36.033745012075414 39.0 35.0 41.0 31.0 41.0 66-67 35.766050380299376 38.5 35.0 41.0 31.0 41.0 68-69 35.46841398856699 37.5 35.0 41.0 30.0 41.0 70-71 35.16532117815541 37.0 35.0 40.0 30.0 41.0 72-73 34.84021896478568 36.5 35.0 39.5 29.5 41.0 74-75 34.50286678074528 36.0 35.0 39.0 29.0 41.0 76-77 34.17204521833685 36.0 35.0 39.0 29.0 41.0 78-79 33.85371808077117 35.0 35.0 37.5 29.0 39.5 80-81 33.59291933064003 35.0 34.5 37.0 29.0 39.0 82-83 33.39232620626247 35.0 35.0 37.0 29.0 39.0 84-85 33.132207586555126 35.0 34.0 36.0 29.0 38.0 86-87 32.89336888341293 35.0 34.0 36.0 29.0 37.0 88-89 32.66365056904073 35.0 34.0 36.0 29.0 37.0 90-91 32.48563237400145 35.0 34.0 35.5 29.0 36.5 92-93 32.32748422030231 35.0 34.0 35.0 29.0 36.0 94-95 32.19159677155112 35.0 34.0 35.0 29.0 36.0 96-97 32.05210866272198 35.0 34.0 35.0 27.5 36.0 98-99 31.940568565260733 35.0 34.0 35.0 27.0 36.0 100 31.84157534951003 35.0 34.0 35.0 27.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.01941499808894065 1101 2 0.02153894181280691 1101 3 0.027277484349092163 1101 4 0.022645757486202456 1101 5 0.023194138155368194 1101 6 0.008502968709144909 1101 7 0.017511504244481557 1101 8 0.07157223361645038 1101 9 0.010640305182107568 1101 10-11 0.058041210306939206 1101 12-13 0.03110907725482548 1101 14-15 0.06688077842640894 1101 16-17 -0.004792603271525309 1101 18-19 -0.050473286435092746 1101 20-21 0.016206324443864162 1101 22-23 0.027133380932326645 1101 24-25 0.03299739917222411 1101 26-27 0.016211870335723688 1101 28-29 -0.03690587755888686 1101 30-31 -0.029704950279679565 1101 32-33 -0.03590652029216557 1101 34-35 -0.011486097985823562 1101 36-37 -0.017359088959850055 1101 38-39 -0.01606672267671172 1101 40-41 -0.05250614227396255 1101 42-43 -0.08343007692256776 1101 44-45 -0.1084331160606169 1101 46-47 -0.05613713139864984 1101 48-49 -0.036640915533602936 1101 50-51 -0.12619787503466995 1101 52-53 -0.08257208214047296 1101 54-55 -0.05122345644407744 1101 56-57 0.02007990598063003 1101 58-59 -0.06308443709954759 1101 60-61 -0.05801049171299155 1101 62-63 -0.004037508442920057 1101 64-65 0.03040106745977056 1101 66-67 -0.025463768819605548 1101 68-69 0.03968787080893321 1101 70-71 0.0704647310216231 1101 72-73 0.031066291328485818 1101 74-75 -0.05300156041338511 1101 76-77 -0.03825243572395465 1101 78-79 0.042625208631179134 1101 80-81 0.05959887079968951 1101 82-83 0.002769474806349592 1101 84-85 0.04090888321455566 1101 86-87 0.06632405355638582 1101 88-89 0.08393598710528494 1101 90-91 0.03053154093694843 1101 92-93 0.06178502467324165 1101 94-95 0.035705211087247335 1101 96-97 0.008905137521701079 1101 98-99 -0.0016695672141615603 1101 100 0.059606323259277616 1102 1 -0.04773613386902298 1102 2 -0.02384901201377687 1102 3 0.016672936817784034 1102 4 0.018915307558685868 1102 5 -0.016388440172335095 1102 6 -0.03326840383942198 1102 7 -0.062342384434089126 1102 8 -0.03148165424051541 1102 9 -0.01291978594876042 1102 10-11 -0.03178992133047842 1102 12-13 -0.06912752433982661 1102 14-15 -0.07124105897455024 1102 16-17 -0.0665863721274107 1102 18-19 -0.05348666555210002 1102 20-21 -0.073455287969594 1102 22-23 -0.0026723478546273327 1102 24-25 -3.156637442529586E-4 1102 26-27 -0.01808188151047574 1102 28-29 -0.023842884061210157 1102 30-31 0.03417378949347949 1102 32-33 -0.008507823273028237 1102 34-35 0.029929065954860334 1102 36-37 -0.034020498669136146 1102 38-39 -0.1026076344500737 1102 40-41 -0.11437398352078532 1102 42-43 -0.11728128795481041 1102 44-45 -0.21915924116182595 1102 46-47 -0.2564930960939762 1102 48-49 -0.2978813366704429 1102 50-51 -0.2944240736305872 1102 52-53 -0.2666103933567143 1102 54-55 -0.2838769092124025 1102 56-57 -0.26329208107352287 1102 58-59 -0.20581468124188973 1102 60-61 -0.26093498396087256 1102 62-63 -0.216379560623011 1102 64-65 -0.26015247538931163 1102 66-67 -0.21575336619315522 1102 68-69 -0.2523996894326501 1102 70-71 -0.30627120424244936 1102 72-73 -0.25482630729915456 1102 74-75 -0.2528975894785148 1102 76-77 -0.291491145108175 1102 78-79 -0.28438741974866133 1102 80-81 -0.3160260106941166 1102 82-83 -0.292732090919678 1102 84-85 -0.2679749126090698 1102 86-87 -0.2783217834918048 1102 88-89 -0.30873713014976545 1102 90-91 -0.2638774891165525 1102 92-93 -0.24516001938956578 1102 94-95 -0.25195093948227765 1102 96-97 -0.2756503710058027 1102 98-99 -0.2590170798260445 1102 100 -0.2184561689339155 1103 1 -0.08923475913839951 1103 2 -0.10197897303492454 1103 3 -0.06351135139600927 1103 4 -0.013722216169767876 1103 5 -0.030637675119542962 1103 6 -0.04292664648106381 1103 7 -0.08287417212433468 1103 8 -0.017678277875077697 1103 9 -0.03859853808516789 1103 10-11 -8.456468977655618E-4 1103 12-13 0.019911447753479194 1103 14-15 0.021937642496105525 1103 16-17 0.0040649867279540786 1103 18-19 -0.05702482361571981 1103 20-21 -0.05704059895595748 1103 22-23 -0.039478649322589376 1103 24-25 -0.040562169496503486 1103 26-27 -0.08945201844309736 1103 28-29 -0.05239573519173035 1103 30-31 -0.054396017774010375 1103 32-33 -0.10853991158004561 1103 34-35 -0.08120726543965873 1103 36-37 -0.08128725045425966 1103 38-39 -0.10207463078187118 1103 40-41 -0.0890353674388038 1103 42-43 -0.09739667075142222 1103 44-45 -0.07529722763745639 1103 46-47 -0.053060998329350184 1103 48-49 -0.07182157810962053 1103 50-51 -0.15677401857380602 1103 52-53 -0.12137500029184167 1103 54-55 -0.09587016729020803 1103 56-57 -0.07732784433612494 1103 58-59 -0.13712341128803018 1103 60-61 -0.11588401421845873 1103 62-63 -0.16810185828089885 1103 64-65 -0.1399736590762899 1103 66-67 -0.14196309536784213 1103 68-69 -0.12910560516537828 1103 70-71 -0.1989577724149001 1103 72-73 -0.2057048513682247 1103 74-75 -0.19255569701150677 1103 76-77 -0.1589579928204543 1103 78-79 -0.14880554052229655 1103 80-81 -0.12889781284098945 1103 82-83 -0.07698720309364404 1103 84-85 -0.1246229342805023 1103 86-87 -0.1182224110715353 1103 88-89 -0.1129724489048769 1103 90-91 -0.12425518537876457 1103 92-93 -0.06552325902503497 1103 94-95 -0.08091620980093239 1103 96-97 -0.0995662928859744 1103 98-99 -0.09964661863263302 1103 100 -0.05184151910178869 1104 1 -0.13354570812764166 1104 2 -0.09703690137063603 1104 3 -0.08186938564000457 1104 4 -0.04524991246147181 1104 5 -0.06526231480935962 1104 6 -0.08251669355876601 1104 7 -0.0988609378402856 1104 8 -0.11569455241655646 1104 9 -0.07518281169699748 1104 10-11 -0.09824116284872275 1104 12-13 -0.10239013482427595 1104 14-15 -0.14627683453332452 1104 16-17 -0.13051638267278065 1104 18-19 -0.09250339017874865 1104 20-21 -0.08458166557802826 1104 22-23 -0.15073497098900646 1104 24-25 -0.14688137952085611 1104 26-27 -0.1565077870434166 1104 28-29 -0.16352380485501072 1104 30-31 -0.163208938017803 1104 32-33 -0.140132607781986 1104 34-35 -0.20899368782759353 1104 36-37 -0.19132161159867422 1104 38-39 -0.2131716176392331 1104 40-41 -0.31845429847371065 1104 42-43 -0.20724462953694456 1104 44-45 -0.1494878228684584 1104 46-47 -0.16541065060817317 1104 48-49 -0.2717660258607495 1104 50-51 -0.369430389179108 1104 52-53 -0.30945748404422346 1104 54-55 -0.2746961158590224 1104 56-57 -0.3073219017571134 1104 58-59 -0.3201223446470607 1104 60-61 -0.29803492549263666 1104 62-63 -0.27573103379293684 1104 64-65 -0.270863118603927 1104 66-67 -0.2806903820089488 1104 68-69 -0.3784890980224702 1104 70-71 -0.2613177698829219 1104 72-73 -0.32977087918403214 1104 74-75 -0.2836197682166386 1104 76-77 -0.30393117563364314 1104 78-79 -0.30079937497848874 1104 80-81 -0.3409180251677313 1104 82-83 -0.3589108508734853 1104 84-85 -0.33804277040136554 1104 86-87 -0.37541557701119643 1104 88-89 -0.33186361468015235 1104 90-91 -0.33953531326231 1104 92-93 -0.3993121113514775 1104 94-95 -0.4370526075777086 1104 96-97 -0.4108167570023369 1104 98-99 -0.4075040024051404 1104 100 -0.317910904616447 1105 1 -0.1813572491228328 1105 2 -0.11087419718672464 1105 3 -0.09178334144215228 1105 4 -0.04412971949939504 1105 5 -0.10018039621964192 1105 6 -0.015444596305407288 1105 7 -0.03931282271333458 1105 8 -0.018756727628286285 1105 9 -0.013987454871404736 1105 10-11 -0.06269290781251158 1105 12-13 -0.03470529134684597 1105 14-15 -0.038742169317693254 1105 16-17 -0.0801578376164187 1105 18-19 -0.09914319965712082 1105 20-21 -0.02404110738906695 1105 22-23 -0.09457343802451135 1105 24-25 -0.11897076757463054 1105 26-27 -0.051101684475717946 1105 28-29 -0.02992267759307765 1105 30-31 -0.04064875896334286 1105 32-33 -0.0741127090581628 1105 34-35 -0.05444102564953823 1105 36-37 -0.07717583545570506 1105 38-39 -0.03782757499899958 1105 40-41 -0.03366442638532163 1105 42-43 -0.07716855418048851 1105 44-45 -0.08716265585024985 1105 46-47 -0.056030794136454176 1105 48-49 0.0048302720812998245 1105 50-51 0.006481639746581891 1105 52-53 -0.04013659038420769 1105 54-55 0.03321566118098218 1105 56-57 0.12930979861947378 1105 58-59 0.05536355520656855 1105 60-61 0.11567845506436214 1105 62-63 0.06279774800533033 1105 64-65 0.038688794410248306 1105 66-67 0.05375006607803812 1105 68-69 0.15235476924112135 1105 70-71 0.1321486144659616 1105 72-73 0.13995671306695812 1105 74-75 0.07743431332988138 1105 76-77 0.024462392803606292 1105 78-79 0.050757722029239005 1105 80-81 0.12656311679079835 1105 82-83 0.07300865155918501 1105 84-85 0.08046709669340402 1105 86-87 0.03487532522346015 1105 88-89 0.08987856072269551 1105 90-91 0.09218146162577057 1105 92-93 0.057291789715769426 1105 94-95 0.03749643313111051 1105 96-97 0.06535615614745183 1105 98-99 0.06888658481315701 1105 100 0.09095419724717857 1106 1 -0.08948877675443612 1106 2 -0.11415937438587065 1106 3 -0.04684228921722422 1106 4 0.002173500032355946 1106 5 -0.0646140323482669 1106 6 -0.08650888285651348 1106 7 -0.03565324617490262 1106 8 -0.050105516527814586 1106 9 -0.061730723065984705 1106 10-11 -0.025964738071579063 1106 12-13 -0.08548302776002714 1106 14-15 -0.07838886269438206 1106 16-17 -0.09273492018929375 1106 18-19 -0.07951331582439991 1106 20-21 -0.10204709172710835 1106 22-23 -0.12032962544711268 1106 24-25 -0.04822620607752981 1106 26-27 -0.11802570252918088 1106 28-29 -0.08265473982844895 1106 30-31 -0.10894069314167609 1106 32-33 -0.13987712999848867 1106 34-35 -0.0756885966226335 1106 36-37 -0.11500420794232014 1106 38-39 -0.051031914134249234 1106 40-41 -0.12992920145283193 1106 42-43 -0.12464344334327393 1106 44-45 -0.1649859790592032 1106 46-47 -0.16209945220983002 1106 48-49 -0.18590555605348413 1106 50-51 -0.2679457537707819 1106 52-53 -0.24885335979536904 1106 54-55 -0.2632697051119166 1106 56-57 -0.19794191053438936 1106 58-59 -0.22101480561296682 1106 60-61 -0.18944163592913554 1106 62-63 -0.24980134346356664 1106 64-65 -0.21097172275585052 1106 66-67 -0.24031017645188513 1106 68-69 -0.2555861094300127 1106 70-71 -0.23566176240476722 1106 72-73 -0.2125542738320334 1106 74-75 -0.21610330112606135 1106 76-77 -0.18498250979809683 1106 78-79 -0.16868286230241836 1106 80-81 -0.18550233307902886 1106 82-83 -0.2298310648582813 1106 84-85 -0.16092038719779822 1106 86-87 -0.15179717780465296 1106 88-89 -0.25830913346631945 1106 90-91 -0.2405390678754742 1106 92-93 -0.22562869577978262 1106 94-95 -0.21467907090104887 1106 96-97 -0.2514904646609928 1106 98-99 -0.31316443128740445 1106 100 -0.22045128831130612 1107 1 -0.19058866758672366 1107 2 -0.19570280421091013 1107 3 -0.1394487562629365 1107 4 -0.128055768800742 1107 5 -0.17031028317914831 1107 6 -0.18586392087978254 1107 7 -0.1113897121840921 1107 8 -0.10083242560765626 1107 9 -0.11668663281434277 1107 10-11 -0.1239357449154852 1107 12-13 -0.11016647230558618 1107 14-15 -0.20818034333375124 1107 16-17 -0.18099922491555276 1107 18-19 -0.20732443785544774 1107 20-21 -0.20450132663548004 1107 22-23 -0.1419944651236733 1107 24-25 -0.16202317291736534 1107 26-27 -0.19920382348472288 1107 28-29 -0.20356115870914238 1107 30-31 -0.21574582711460977 1107 32-33 -0.24281987971249208 1107 34-35 -0.23630616682326888 1107 36-37 -0.28507101373125465 1107 38-39 -0.22554896231733323 1107 40-41 -0.30909841782266056 1107 42-43 -0.2587439817645105 1107 44-45 -0.3301118286036555 1107 46-47 -0.3951932560864222 1107 48-49 -0.40343782019941443 1107 50-51 -0.3514052052886214 1107 52-53 -0.377916778342275 1107 54-55 -0.38080941629295495 1107 56-57 -0.41270305148911035 1107 58-59 -0.45229237687865975 1107 60-61 -0.5045643236039368 1107 62-63 -0.42910167044368563 1107 64-65 -0.3467006834849471 1107 66-67 -0.34518371711828877 1107 68-69 -0.33906847261614814 1107 70-71 -0.3647146314805383 1107 72-73 -0.36515371106677463 1107 74-75 -0.3573606007657517 1107 76-77 -0.2916568897625851 1107 78-79 -0.3371325678919348 1107 80-81 -0.3341461049565453 1107 82-83 -0.37970977862872246 1107 84-85 -0.3190587425310909 1107 86-87 -0.32973516688407756 1107 88-89 -0.3020291729682967 1107 90-91 -0.3635010883529759 1107 92-93 -0.3707493900502854 1107 94-95 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0.20412599413207033 2214 70-71 0.18260666256700375 2214 72-73 0.15038831823862608 2214 74-75 0.17829572342444777 2214 76-77 0.21085455853339852 2214 78-79 0.20601981447174467 2214 80-81 0.12005817931130025 2214 82-83 0.08560584237145719 2214 84-85 0.1022626275731966 2214 86-87 0.11856490299305733 2214 88-89 0.12159802872445624 2214 90-91 0.13774508995088297 2214 92-93 0.13524816435353415 2214 94-95 0.11936761233720006 2214 96-97 0.1598334695407786 2214 98-99 0.15521903510219914 2214 100 0.22065296754364283 2215 1 0.05943501673214513 2215 2 0.0534119226296923 2215 3 0.0349719803951416 2215 4 0.02140345583205061 2215 5 0.004717521981632444 2215 6 0.01668666982013889 2215 7 0.025100683461111828 2215 8 0.012341121602347016 2215 9 0.016691054093193713 2215 10-11 0.026071318291727152 2215 12-13 0.04475659842488966 2215 14-15 0.08680536113278237 2215 16-17 0.032746931834992665 2215 18-19 0.03180159011668593 2215 20-21 0.011932254193247616 2215 22-23 -0.016363348810941147 2215 24-25 0.02222552299113545 2215 26-27 0.04735138990448462 2215 28-29 0.1024783885170848 2215 30-31 0.08236525088692304 2215 32-33 0.0711587736376984 2215 34-35 0.08785475400468101 2215 36-37 0.054934333797675095 2215 38-39 0.06386564702761177 2215 40-41 0.06390381562008685 2215 42-43 0.08015501345408893 2215 44-45 0.0762233861599313 2215 46-47 0.19768271506629986 2215 48-49 0.20992933388211554 2215 50-51 0.23419206578808627 2215 52-53 0.19726620310865428 2215 54-55 0.1788512681509502 2215 56-57 0.19067130966561052 2215 58-59 0.21587405432225637 2215 60-61 0.19925875399844273 2215 62-63 0.15656860496324043 2215 64-65 0.13338003910181584 2215 66-67 0.1468259053728076 2215 68-69 0.17900316008972794 2215 70-71 0.1977324996865093 2215 72-73 0.16911688614077747 2215 74-75 0.1808050912089314 2215 76-77 0.21705599338961434 2215 78-79 0.2730714860982104 2215 80-81 0.27664603947143007 2215 82-83 0.281735892298677 2215 84-85 0.2919855499765944 2215 86-87 0.30252028888868665 2215 88-89 0.2556217879722382 2215 90-91 0.2509350741047669 2215 92-93 0.21131757833699538 2215 94-95 0.16881947582419343 2215 96-97 0.21594447656787708 2215 98-99 0.23495122575415905 2215 100 0.2527832313948153 2216 1 0.0703462259142853 2216 2 0.056172623795916365 2216 3 0.02073679039930454 2216 4 0.0167727605516248 2216 5 0.014661679613773515 2216 6 0.018100062658852778 2216 7 0.04541137880272572 2216 8 0.017496924281260817 2216 9 -0.00873249218138028 2216 10-11 -0.008170240543115881 2216 12-13 0.01728137342529834 2216 14-15 -0.01005410789187522 2216 16-17 -0.030436360980942823 2216 18-19 -0.05704524344746176 2216 20-21 -0.06757314816774596 2216 22-23 -0.14363612706496554 2216 24-25 -0.04953260158014672 2216 26-27 -0.024633346548959878 2216 28-29 -0.0558221829578045 2216 30-31 -0.09493550972860021 2216 32-33 0.019118750512582494 2216 34-35 0.08986395893344934 2216 36-37 0.04220780794645407 2216 38-39 0.13539371847892312 2216 40-41 0.0468729066770166 2216 42-43 0.06283774891696225 2216 44-45 0.07444139851626375 2216 46-47 0.05756752409162402 2216 48-49 0.11262667803674731 2216 50-51 0.14309937846940102 2216 52-53 0.10593039871167065 2216 54-55 0.1778556352091769 2216 56-57 0.13539983678778356 2216 58-59 0.1285100118925584 2216 60-61 0.09648223588364147 2216 62-63 0.05088041730515158 2216 64-65 0.009630196187949025 2216 66-67 0.02270651175459193 2216 68-69 -0.019549123910785227 2216 70-71 0.01906477107934279 2216 72-73 0.03113048310861899 2216 74-75 0.0333325656692125 2216 76-77 0.0771769414276875 2216 78-79 0.047145206843545395 2216 80-81 0.06533154270911723 2216 82-83 0.057916415300546475 2216 84-85 0.08328158258628093 2216 86-87 0.06834746559268012 2216 88-89 0.10422717537021953 2216 90-91 0.07316987383875073 2216 92-93 0.06355866533990451 2216 94-95 0.07030693812032496 2216 96-97 0.07016539052495574 2216 98-99 0.03699064347632941 2216 100 0.031259028149701606 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40.0 3 286.0 4 82.0 5 125.0 6 258.0 7 671.0 8 1296.0 9 2158.0 10 3126.0 11 4396.0 12 6422.0 13 9527.0 14 10918.0 15 14653.0 16 19156.0 17 29409.0 18 34360.0 19 18605.0 20 11631.0 21 10334.0 22 10498.0 23 10711.0 24 11201.0 25 12918.0 26 16258.0 27 20564.0 28 25518.0 29 32536.0 30 41088.0 31 51331.0 32 65388.0 33 85351.0 34 118717.0 35 196490.0 36 327184.0 37 680073.0 38 1213104.0 39 765719.0 40 7744.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.52798146585642 20.99738604486493 14.815892537112866 22.658739952165785 2 14.431747409985865 21.956135794525114 40.30723550070161 23.30488129478741 3 16.530518795228296 28.054282740350178 32.06047218831302 23.354726276108504 4 11.219722955383753 18.594667591423537 39.50446606919242 30.681143384000293 5 11.504495931157097 39.07779960615845 35.52438397302209 13.89332048966237 6 27.534997516697047 39.89246600510718 18.377759735141915 14.194776743053858 7 24.31711752870786 35.3511224994483 23.262656963610436 17.0691030082334 8 24.9806581450528 35.22822355204833 20.93047113502708 18.860647167871797 9 25.820717413561162 16.009525960464575 22.07095579514017 36.098800830834094 10-11 22.96521618689736 27.63277918552831 29.22673150301072 20.175273124563613 12-13 24.532478105392062 25.953645953049033 29.435645428221875 20.07823051333703 14-15 21.813173395833683 26.919799113401183 28.261894210233223 23.005133280531908 16-17 19.482739106414055 30.22807625936536 28.93653907674879 21.352645557471796 18-19 19.449153221242625 27.77524250344071 32.970364164738506 19.805240110578158 20-21 20.914695313456917 26.80368934577758 32.89718764002495 19.38442770074055 22-23 20.521041540423454 26.904294616451047 32.93453613775228 19.64012770537321 24-25 20.240270636197398 27.000942802740525 32.83148715665449 19.92729940440759 26-27 19.966158811971706 27.058621666706934 32.91751946882565 20.057700052495704 28-29 19.930002301127836 26.996041052327335 33.11030955660587 19.963647089938956 30-31 20.1235427988753 27.180178059363286 32.836378210635964 19.859900931125452 32-33 19.927988219496 27.184169346941502 32.96847226724699 19.919370166315506 34-35 20.26335200914052 27.016644135364565 32.73864305443057 19.981360801064344 36-37 20.37261209495041 27.084283805174366 32.379327707444475 20.163776392430748 38-39 20.222836035801368 27.241371379783324 32.41593355308652 20.119859031328783 40-41 20.19626408037341 27.076114575368422 32.42560345564943 20.302017888608745 42-43 20.244336914334916 27.216805017183614 32.20396139820956 20.334896670271917 44-45 20.44500048257828 27.316139937105905 31.857014188059786 20.381845392256036 46-47 20.598810286730203 27.535027287949887 31.33731627147376 20.528846153846153 48-49 20.712047618512344 27.666328280539798 30.80392610597933 20.817697994968526 50-51 20.684825594496093 27.791128917224313 30.58435094856831 20.939694539711283 52-53 20.706677283885313 27.950254463101526 30.512896677605607 20.830171575407554 54-55 20.720138249592196 28.13436051488283 30.465003149377388 20.680498086147583 56-57 20.701404076964334 27.903259796165997 30.395193094983895 21.000143031885774 58-59 20.685360219689862 27.653218406338905 30.663591759717963 20.997829614253277 60-61 20.733349966451662 27.813140740601007 30.565331783550498 20.88817750939683 62-63 20.574214176313824 27.68493043426354 30.78012183080594 20.960733558616703 64-65 20.604435111725387 27.59956416110121 30.82120850069326 20.974792226480147 66-67 20.54536358519849 27.632769672610262 30.67911133897115 21.142755403220097 68-69 20.878820869014245 27.759505384340876 30.38680222118112 20.974871525463758 70-71 20.812332211751773 27.698275351933795 30.390032334728655 21.09936010158578 72-73 20.78488815294077 27.784806495775793 30.419141576469094 21.011163774814346 74-75 20.809976808965907 27.847936897233705 30.25104950120994 21.091036792590444 76-77 20.85292784932806 27.816044786677598 30.099463500999985 21.23156386299436 78-79 20.834962399894774 27.899882758889795 30.17613558106188 21.089019260153552 80-81 20.67847087902362 27.854917754253766 30.23918517858135 21.227426188141262 82-83 20.64584700231616 27.858237606307974 30.368269853694187 21.127645537681687 84-85 20.691116093301332 27.851812917723574 30.08297103921046 21.374099949764638 86-87 20.7762755312991 27.872271148598365 30.026371144463738 21.325082175638798 88-89 20.643497866484918 27.78422587803594 30.12795922256713 21.444317032912014 90-91 20.561344545632615 27.782535419161412 30.063171255967365 21.592948779238604 92-93 20.595846671167344 27.82128217634689 30.041920906509723 21.54095024597604 94-95 20.479884397335578 27.711924651539473 30.15499222571048 21.653198725414473 96-97 20.54893742376946 27.872948235585966 29.899789138569037 21.678325202075538 98-99 20.421860527074358 27.800433791272933 29.980062449715213 21.797643231937496 100 20.473435716576322 27.85139123358879 29.875454766981264 21.79971828285363 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2244.0 1 1363.0 2 2102.5 3 3793.5 4 2417.5 5 1421.0 6 3832.5 7 9934.5 8 17560.5 9 19591.5 10 12516.0 11 5705.0 12 4552.5 13 4754.0 14 5188.5 15 5631.5 16 6108.0 17 6756.5 18 7494.5 19 8391.5 20 9707.0 21 11870.0 22 15146.5 23 20290.5 24 26442.5 25 32642.5 26 40872.0 27 50519.5 28 63036.0 29 75757.5 30 83619.0 31 91261.5 32 99625.0 33 106699.5 34 113633.0 35 119322.0 36 121796.0 37 124004.5 38 125873.5 39 125593.0 40 126239.0 41 125573.0 42 123183.0 43 121928.5 44 121537.0 45 120075.0 46 117942.5 47 115714.0 48 111509.0 49 108596.5 50 108411.5 51 104198.0 52 96516.0 53 90607.5 54 86803.0 55 83653.5 56 76462.5 57 67745.5 58 61741.5 59 56955.5 60 50093.5 61 41559.5 62 35407.0 63 29671.5 64 23404.0 65 18788.5 66 14885.0 67 11258.0 68 8565.0 69 6271.5 70 4392.0 71 3397.5 72 2695.0 73 1879.0 74 1405.0 75 1040.0 76 605.5 77 459.5 78 363.0 79 183.0 80 79.0 81 41.0 82 21.0 83 15.0 84 12.0 85 3.5 86 1.5 87 2.5 88 1.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.5 94 1.0 95 1.0 96 0.5 97 0.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005969229783304038 2 0.004547984596803077 3 8.785879334733217E-4 4 0.0 5 0.046332593079931345 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 1.2920410786372378E-5 14-15 2.5840821572744755E-5 16-17 0.0 18-19 1.0336328629097902E-4 20-21 0.0 22-23 2.5840821572744755E-5 24-25 3.876123235911713E-5 26-27 1.4212451865009616E-4 28-29 1.162836970773514E-4 30-31 3.359306804456818E-4 32-33 0.0016150513482965471 34-35 0.0011499165599871417 36-37 0.0014858472404328236 38-39 0.0012920410786372378 40-41 2.325673941547028E-4 42-43 6.589409501049913E-4 44-45 1.9380616179558564E-4 46-47 1.5504492943646853E-4 48-49 1.679653402228409E-4 50-51 3.6177150201842655E-4 52-53 8.139858795414599E-4 54-55 8.656675226869493E-4 56-57 0.0020543453150332082 58-59 6.977021824641083E-4 60-61 0.0014600064188600788 62-63 0.001369563543355472 64-65 0.0017442554561602711 66-67 6.201797177458741E-4 68-69 3.359306804456818E-4 70-71 3.359306804456818E-4 72-73 2.0672657258195804E-4 74-75 8.139858795414599E-4 76-77 8.915083442596939E-4 78-79 0.0011111553276280245 80-81 0.001059473684482535 82-83 0.0019380616179558567 84-85 0.0016021309375101747 86-87 0.002222310655256049 88-89 0.0015116880620055682 90-91 0.0012403594354917483 92-93 0.0015116880620055682 94-95 0.0010077920413370453 96-97 1.5504492943646853E-4 98-99 3.2301026965930946E-4 100 1.8088575100921327E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3869846.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.27577986809341 #Duplication Level Percentage of deduplicated Percentage of total 1 72.53955031098019 40.09680214720228 2 16.281180740018026 17.99909925155757 3 5.210042772374336 8.639675321673446 4 2.1985876571703464 4.861145894338211 5 1.086428637913826 3.00265951158586 6 0.6413680670749531 2.1271272054035806 7 0.4054513725701825 1.568814857218401 8 0.29286344358361516 1.295060419115176 9 0.2100349606007346 1.0448861622092889 >10 1.0306345530174768 10.140868275081985 >50 0.06636675762844522 2.5391504819017525 >100 0.03510300340928996 3.253403877631066 >500 0.0015449976876672827 0.5592209569873828 >1k 6.086354233638624E-4 0.6492542371081618 >5k 9.363621897905479E-5 0.29018206954295994 >10k+ 1.404543284685822E-4 1.9326493314428115 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31917 0.8247615021372943 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25913 0.6696132094145348 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16850 0.43541784350074914 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5810 0.150135173337647 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5403 0.13961795895753992 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4672 0.12072831838786349 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4008 0.10357001286356099 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.5840821572744755E-5 0.0 5 0.0 0.0 0.0 5.168164314548951E-5 0.0 6 0.0 0.0 0.0 5.168164314548951E-5 0.0 7 0.0 0.0 0.0 5.168164314548951E-5 0.0 8 0.0 0.0 0.0 5.168164314548951E-5 0.0 9 0.0 0.0 0.0 5.168164314548951E-5 0.0 10-11 0.0 0.0 0.0 5.168164314548951E-5 0.0 12-13 0.0 0.0 0.0 6.460205393186188E-5 0.0 14-15 0.0 0.0 0.0 9.044287550460665E-5 0.0 16-17 0.0 0.0 0.0 1.0336328629097902E-4 0.0 18-19 0.0 0.0 0.0 1.0336328629097902E-4 0.0 20-21 0.0 0.0 0.0 1.1628369707735139E-4 0.0 22-23 0.0 0.0 0.0 2.454878049410752E-4 0.0 24-25 0.0 0.0 0.0 5.297368422412674E-4 0.0 26-27 0.0 0.0 0.0 0.001020712452123418 0.0 28-29 0.0 0.0 0.0 0.002493639281769869 0.0 30-31 0.0 0.0 0.0 0.006150115534313251 0.0 32-33 0.0 0.0 0.0 0.012571559695140323 0.0 34-35 0.0 0.0 0.0 0.022752843394801756 0.0 36-37 0.0 0.0 0.0 0.04040212452898642 0.0 38-39 0.0 0.0 0.0 0.06411107832197974 0.0 40-41 0.0 0.0 0.0 0.08928003853383312 0.0 42-43 0.0 0.0 0.0 0.11400970477894987 1.2920410786372378E-5 44-45 0.0 0.0 0.0 0.1441530231435566 2.5840821572744755E-5 46-47 0.0 0.0 0.0 0.18482647629905685 2.5840821572744755E-5 48-49 0.0 0.0 0.0 0.22779976257453138 2.5840821572744755E-5 50-51 0.0 0.0 0.0 0.27212969198257503 2.5840821572744755E-5 52-53 0.0 0.0 0.0 0.31636917851511404 2.5840821572744755E-5 54-55 0.0 0.0 0.0 0.3660610783995022 2.5840821572744755E-5 56-57 0.0 0.0 0.0 0.42802736853094414 3.876123235911713E-5 58-59 0.0 0.0 0.0 0.49818519910094616 5.168164314548951E-5 60-61 0.0 0.0 0.0 0.5730589796079741 5.168164314548951E-5 62-63 0.0 0.0 0.0 0.6519251670479911 5.168164314548951E-5 64-65 0.0 0.0 0.0 0.7338147306120192 5.168164314548951E-5 66-67 0.0 0.0 0.0 0.8322036587502448 5.168164314548951E-5 68-69 0.0 0.0 0.0 0.9473632800891818 7.752246471823427E-5 70-71 0.0 0.0 0.0 1.0752624264634818 9.044287550460665E-5 72-73 0.0 0.0 0.0 1.2088207127622133 1.0336328629097902E-4 74-75 0.0 0.0 0.0 1.3479864573422302 1.0336328629097902E-4 76-77 0.0 0.0 0.0 1.501442176251975 1.0336328629097902E-4 78-79 0.0 0.0 0.0 1.6792270286724587 1.0336328629097902E-4 80-81 0.0 0.0 0.0 1.880617471599645 1.0336328629097902E-4 82-83 0.0 0.0 0.0 2.0969568298066643 1.0336328629097902E-4 84-85 0.0 0.0 0.0 2.3163583253700537 1.0336328629097902E-4 86-87 0.0 0.0 0.0 2.5510963485368667 1.2920410786372376E-4 88 0.0 0.0 0.0 2.743184095697865 1.2920410786372376E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10330 0.0 66.183815 1 GTATCAA 20370 0.0 45.45089 1 GTGGTAT 3045 0.0 36.44286 1 ATCAACG 26380 0.0 34.91333 3 TCAACGC 26610 0.0 34.646896 4 TATCAAC 26670 0.0 34.553555 2 CAACGCA 27655 0.0 33.320694 5 TGGTATC 3175 0.0 33.319496 2 AACGCAG 28415 0.0 32.550488 6 ACGCAGA 32120 0.0 28.722672 7 CGCAGAG 32095 0.0 28.657185 8 GCAGAGT 34800 0.0 26.173403 9 GTACATG 18325 0.0 25.736214 1 CATGGGT 7445 0.0 25.385921 4 TACATGG 18295 0.0 25.134258 2 ACATGGG 18370 0.0 24.287828 3 GAGTACT 24185 0.0 23.436184 12-13 CAGAGTA 34320 0.0 22.663992 10-11 AGAGTAC 32935 0.0 22.068758 10-11 AGTACTT 24805 0.0 21.334616 12-13 >>END_MODULE