FastQCFastQC Report
Wed 25 May 2016
SRR2431394_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431394_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4208269
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA137580.3269277700641285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52700.049.4536131
GTATCAA101050.039.7576261
ATCAACG112000.034.272043
TCAACGC112600.034.0882034
CAACGCA121500.031.6291685
TATCAAC125600.031.3850062
AACGCAG130750.029.6169286
GTACATG206900.026.9891411
TACATGG206850.026.243282
CGCAGAG143850.026.135428
CATGGGT97750.025.7447914
ACGCAGA150700.025.3531427
ACATGGG210750.024.7978693
GCAGAGT161300.022.7252989
ATGGGTG57850.021.384275
GTGGTAT28200.020.5189381
TACCTGG33250.019.3819182
ATGGGTA41800.019.2425165
GTACCTG35700.018.8437231
CAGAGTA159800.018.6596810-11
Produced by FastQC (version 0.11.5)