##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431394_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4208269 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.882024176686425 33.0 31.0 34.0 27.0 34.0 2 31.02473273452814 33.0 31.0 34.0 27.0 34.0 3 31.05380953546458 34.0 31.0 34.0 27.0 34.0 4 34.25704891963893 37.0 35.0 37.0 30.0 37.0 5 34.35684387095977 37.0 35.0 37.0 31.0 37.0 6 34.33600632469074 37.0 35.0 37.0 31.0 37.0 7 34.29720889990635 37.0 35.0 37.0 31.0 37.0 8 34.27119487846428 37.0 35.0 37.0 31.0 37.0 9 35.71513774428393 39.0 37.0 39.0 30.0 39.0 10-11 35.74221657408307 39.0 37.0 39.0 30.0 39.0 12-13 35.689025345100326 39.0 37.0 39.0 30.0 39.0 14-15 37.04564739088684 41.0 38.0 41.0 30.0 41.0 16-17 37.06896921275707 41.0 38.0 41.0 30.0 41.0 18-19 37.104952891557076 40.5 38.0 41.0 30.0 41.0 20-21 37.208521722351875 41.0 38.0 41.0 30.0 41.0 22-23 37.180531591492844 40.5 38.0 41.0 30.0 41.0 24-25 37.22908017524546 40.5 38.0 41.0 30.0 41.0 26-27 37.273545251028395 41.0 38.0 41.0 30.0 41.0 28-29 37.24121259833913 40.0 38.0 41.0 30.0 41.0 30-31 37.1707294614484 40.0 38.0 41.0 30.0 41.0 32-33 37.12475913493172 40.0 38.0 41.0 30.0 41.0 34-35 37.05326275482865 40.0 38.0 41.0 30.0 41.0 36-37 36.91665266645265 40.0 38.0 41.0 30.0 41.0 38-39 36.796237716742915 40.0 37.0 41.0 30.0 41.0 40-41 36.741072754617164 40.0 37.0 41.0 30.0 41.0 42-43 36.56967080764086 40.0 37.0 41.0 29.0 41.0 44-45 36.40885230958382 40.0 37.0 41.0 29.0 41.0 46-47 36.28108706929144 40.0 36.0 41.0 29.0 41.0 48-49 36.14629007793941 40.0 36.0 41.0 28.0 41.0 50-51 36.0137523290455 40.0 36.0 41.0 28.0 41.0 52-53 35.85868655259443 40.0 35.0 41.0 28.0 41.0 54-55 35.70101590463918 40.0 35.0 41.0 28.0 41.0 56-57 35.518577353301325 39.0 35.0 41.0 27.5 41.0 58-59 35.31353200092485 39.0 35.0 41.0 27.0 41.0 60-61 35.084816940171834 39.0 34.5 41.0 26.5 41.0 62-63 34.99565866630674 39.0 34.5 41.0 26.5 41.0 64-65 34.76027102830166 38.0 34.0 40.5 26.0 41.0 66-67 34.50922481428825 38.0 34.0 40.0 26.0 41.0 68-69 34.226952459550475 37.0 34.0 40.0 26.0 41.0 70-71 33.90695687466747 37.0 34.0 40.0 26.0 41.0 72-73 33.57161198583076 36.0 33.5 39.0 25.0 41.0 74-75 33.2822236411218 36.0 33.0 39.0 25.0 41.0 76-77 33.01090531522581 35.5 33.0 39.0 24.5 40.5 78-79 32.65511187616571 35.0 33.0 37.5 24.0 39.5 80-81 32.31821183484231 35.0 33.0 37.0 23.0 39.0 82-83 31.978789735161893 35.0 33.0 37.0 22.5 39.0 84-85 31.662638842716564 35.0 32.0 36.0 21.5 38.0 86-87 31.36915807901063 35.0 32.0 36.0 20.0 37.0 88-89 31.090842101586187 35.0 32.0 35.5 19.5 37.0 90-91 30.848481525301736 35.0 32.0 35.0 18.0 36.5 92-93 30.610041325780266 35.0 32.0 35.0 15.0 36.0 94-95 30.391268119979973 35.0 31.5 35.0 4.5 36.0 96-97 30.18841939524303 35.0 31.0 35.0 2.0 36.0 98-99 29.97182143061672 35.0 31.0 35.0 2.0 35.5 100 29.800080270534036 35.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2832659306296925 1101 2 0.2608171314813177 1101 3 0.27632205876081173 1101 4 0.34351811178451186 1101 5 0.3308087846426204 1101 6 0.4025475560186891 1101 7 0.3104247943958143 1101 8 0.34404044745448203 1101 9 0.34124637045662354 1101 10-11 0.3970391008840721 1101 12-13 0.30008153406219407 1101 14-15 0.3353991799042504 1101 16-17 0.27134531276577434 1101 18-19 0.2551728035782048 1101 20-21 0.2678645836756246 1101 22-23 0.3061449028651282 1101 24-25 0.2695472855568468 1101 26-27 0.27055988119403906 1101 28-29 0.25665287836108774 1101 30-31 0.3151428443333444 1101 32-33 0.30924994273503614 1101 34-35 0.29399367821285693 1101 36-37 0.26951911529936723 1101 38-39 0.31671523893071196 1101 40-41 0.2740432912608526 1101 42-43 0.2311722184595979 1101 44-45 0.2295978933645415 1101 46-47 0.20920406010137782 1101 48-49 0.24926915169510266 1101 50-51 0.28251037520183075 1101 52-53 0.2483350683564609 1101 54-55 0.2622378146341191 1101 56-57 0.17339334759419245 1101 58-59 0.15220809620446119 1101 60-61 0.13842404705036415 1101 62-63 0.12787255058474045 1101 64-65 0.21187885939209394 1101 66-67 0.2527758854811495 1101 68-69 0.2390572711987815 1101 70-71 0.25203730372745525 1101 72-73 0.1918392979654513 1101 74-75 0.2808170421803311 1101 76-77 0.2303987584972802 1101 78-79 0.1994782380537714 1101 80-81 0.17303545783644836 1101 82-83 0.23077598846859715 1101 84-85 0.12733095520300708 1101 86-87 0.1105863345316287 1101 88-89 0.08484063496615946 1101 90-91 0.08943668247023595 1101 92-93 -0.003725249810031528 1101 94-95 0.08140488648723476 1101 96-97 0.057750389311514994 1101 98-99 0.07721401398531214 1101 100 0.06724113407044996 1102 1 8.076314773823867E-4 1102 2 0.037153920205309277 1102 3 0.05677797145626684 1102 4 0.04266482916338532 1102 5 0.038196235856972294 1102 6 0.0766911660318641 1102 7 -0.01625753339397562 1102 8 0.007817264020474113 1102 9 0.02481213474428756 1102 10-11 0.057642364860996054 1102 12-13 -0.006799105806557293 1102 14-15 0.004990188430859632 1102 16-17 0.011346431417429415 1102 18-19 -0.021387715476166136 1102 20-21 -0.009254328994963146 1102 22-23 -0.018969758929188174 1102 24-25 -0.005858536403529513 1102 26-27 0.04368023568231649 1102 28-29 0.007710314908678129 1102 30-31 0.040519457056142016 1102 32-33 0.04298132961449852 1102 34-35 0.03359301447954266 1102 36-37 -0.07317298204818456 1102 38-39 -0.0365586815704404 1102 40-41 -0.023346832288005714 1102 42-43 -0.05352493767931321 1102 44-45 -0.09704869971970709 1102 46-47 -0.15450843399462855 1102 48-49 -0.1937809141810547 1102 50-51 -0.23050078645576377 1102 52-53 -0.17677524312587423 1102 54-55 -0.17938472743961142 1102 56-57 -0.19582322336560765 1102 58-59 -0.2308747834534799 1102 60-61 -0.26024848632273745 1102 62-63 -0.1187780199276034 1102 64-65 -0.10839173001303948 1102 66-67 -0.17849415702760751 1102 68-69 -0.25701128971800813 1102 70-71 -0.2978250399720537 1102 72-73 -0.27005880529852533 1102 74-75 -0.18617434420195167 1102 76-77 -0.16893968334526477 1102 78-79 -0.18891761547743613 1102 80-81 -0.24581413323422652 1102 82-83 -0.2678561491504148 1102 84-85 -0.33625600131873057 1102 86-87 -0.3092927267953378 1102 88-89 -0.24905295640282077 1102 90-91 -0.2113504132614885 1102 92-93 -0.2355372168968124 1102 94-95 -0.305655421266259 1102 96-97 -0.3255133390301488 1102 98-99 -0.3665871021804499 1102 100 -0.45189961716628346 1103 1 -0.2629052215711667 1103 2 -0.17197796302621882 1103 3 -0.1738670754791336 1103 4 -0.13520855856724978 1103 5 -0.07160918699658936 1103 6 -0.08474341388097884 1103 7 -0.20242747777440684 1103 8 -0.1990017768627581 1103 9 -0.11870870965358904 1103 10-11 -0.18129948339461777 1103 12-13 -0.1625803580493681 1103 14-15 -0.16338077086424363 1103 16-17 -0.24733561708081453 1103 18-19 -0.24539914908827143 1103 20-21 -0.2770788141757663 1103 22-23 -0.20997507056102194 1103 24-25 -0.20463901200803747 1103 26-27 -0.2429378198641814 1103 28-29 -0.21550346593012648 1103 30-31 -0.19649816704396272 1103 32-33 -0.21469591181671177 1103 34-35 -0.15666197451355401 1103 36-37 -0.2260209349287905 1103 38-39 -0.1953021586568937 1103 40-41 -0.20669260031608871 1103 42-43 -0.2431140425161331 1103 44-45 -0.44359551376278716 1103 46-47 -0.5392177448678694 1103 48-49 -0.4673929695387784 1103 50-51 -0.5480022033693857 1103 52-53 -0.5474074138723424 1103 54-55 -0.5406436557353018 1103 56-57 -0.560689300561414 1103 58-59 -0.5243240644704059 1103 60-61 -0.5530645059068178 1103 62-63 -0.43294536732176425 1103 64-65 -0.48448937591381736 1103 66-67 -0.49047667907731096 1103 68-69 -0.5229121488265633 1103 70-71 -0.5357623258310582 1103 72-73 -0.5225188305006654 1103 74-75 -0.5293021880878612 1103 76-77 -0.4630319310552622 1103 78-79 -0.4937988230923622 1103 80-81 -0.45025766895458474 1103 82-83 -0.505650085325577 1103 84-85 -0.5164264909336111 1103 86-87 -0.5790494286988981 1103 88-89 -0.62206055075535 1103 90-91 -0.6097575707305047 1103 92-93 -0.7198161089943262 1103 94-95 -0.6768774924090266 1103 96-97 -0.6076404145421819 1103 98-99 -0.6895918909787611 1103 100 -0.6683455693605076 1104 1 -0.1917135607566891 1104 2 -0.1513239677803746 1104 3 -0.1289281463253218 1104 4 -0.11275752398250916 1104 5 -0.1768204196231764 1104 6 -0.23403981067531987 1104 7 -0.12124050502994521 1104 8 -0.15440089791056977 1104 9 -0.2385731537435447 1104 10-11 -0.23224719936203542 1104 12-13 -0.24249434780819712 1104 14-15 -0.20118818678975714 1104 16-17 -0.1604840555689222 1104 18-19 -0.21045475999849828 1104 20-21 -0.24050293068039963 1104 22-23 -0.12002408811108012 1104 24-25 -0.11946009754405651 1104 26-27 -0.10294299001186857 1104 28-29 -0.011517753304204348 1104 30-31 -0.07033705722530925 1104 32-33 -0.10748508597702511 1104 34-35 -0.05227375410872526 1104 36-37 -0.09143248437249696 1104 38-39 -0.12380978157790423 1104 40-41 -0.11258509266039596 1104 42-43 -0.20713787177830056 1104 44-45 -0.18049234453537366 1104 46-47 -0.3379657512193859 1104 48-49 -0.34228129949440245 1104 50-51 -0.39017215145936746 1104 52-53 -0.44469200301351464 1104 54-55 -0.3270156718301962 1104 56-57 -0.32357137759038324 1104 58-59 -0.3737147914083394 1104 60-61 -0.4139959693565203 1104 62-63 -0.34628659625693103 1104 64-65 -0.3056765138646753 1104 66-67 -0.26683034913738624 1104 68-69 -0.21615191666011668 1104 70-71 -0.27068624016098397 1104 72-73 -0.26697940589016866 1104 74-75 -0.20638558292221632 1104 76-77 -0.1881410282131455 1104 78-79 -0.1927284887714933 1104 80-81 -0.21859702780751888 1104 82-83 -0.15987208372171935 1104 84-85 -0.19178307268870398 1104 86-87 -0.10442597063653736 1104 88-89 -0.19153344214622692 1104 90-91 -0.21704403943296313 1104 92-93 -0.26927077893226326 1104 94-95 -0.20046549907469569 1104 96-97 -0.16612163693951132 1104 98-99 -0.20334382030509346 1104 100 -0.15654885772610427 1105 1 -0.15849842689197757 1105 2 -0.10265359574716015 1105 3 -0.04898494042945245 1105 4 0.043657311719790926 1105 5 -0.0013840686112089884 1105 6 -0.04516254204465042 1105 7 -0.020098853345452028 1105 8 -0.03406053118936825 1105 9 -0.03487410944162406 1105 10-11 -0.07287388423500119 1105 12-13 -0.02093261605224228 1105 14-15 -0.07377163545987031 1105 16-17 -0.10002519316198999 1105 18-19 -0.05608660767039453 1105 20-21 -0.1034935872411964 1105 22-23 -0.1383374676852327 1105 24-25 -0.17142650387125258 1105 26-27 -0.06338136038449704 1105 28-29 -0.046766108138704965 1105 30-31 -0.028987845770721776 1105 32-33 -0.06859637761846216 1105 34-35 -0.10255649776000553 1105 36-37 -0.11201490910405454 1105 38-39 -0.1409127384657083 1105 40-41 -0.2041982136635383 1105 42-43 -0.2357863989050486 1105 44-45 -0.32126618741519053 1105 46-47 -0.32622805986920866 1105 48-49 -0.34400349250018536 1105 50-51 -0.3210266560263051 1105 52-53 -0.30981907020549926 1105 54-55 -0.3334643624953202 1105 56-57 -0.3668760861823799 1105 58-59 -0.3040947959577025 1105 60-61 -0.3739581817800044 1105 62-63 -0.2738419496185287 1105 64-65 -0.22827107067635666 1105 66-67 -0.22811439724923588 1105 68-69 -0.2092921915982231 1105 70-71 -0.17716537044986325 1105 72-73 -0.13061983899495289 1105 74-75 -0.18031295092930577 1105 76-77 -0.1941559376602342 1105 78-79 -0.179330478422024 1105 80-81 -0.17619708563617564 1105 82-83 -0.19312130465967314 1105 84-85 -0.20804917203329154 1105 86-87 -0.15562568308488878 1105 88-89 -0.15663650248896488 1105 90-91 -0.15940777237864978 1105 92-93 -0.18053025649812682 1105 94-95 -0.29307251690011427 1105 96-97 -0.24513202421243108 1105 98-99 -0.316220775918282 1105 100 -0.2608736054055001 1106 1 -0.0689673677130962 1106 2 -0.017578376183131184 1106 3 -0.014444810212122405 1106 4 0.10305941533372476 1106 5 0.11043465202016023 1106 6 0.07006028864056901 1106 7 0.10743267080317054 1106 8 0.025583114142449404 1106 9 0.03228055182843548 1106 10-11 0.027124890123339185 1106 12-13 0.10898709022725228 1106 14-15 -0.02173788309470126 1106 16-17 0.014624837117807488 1106 18-19 -0.05297672902440809 1106 20-21 -0.020639333580852792 1106 22-23 -2.3629367534283574E-4 1106 24-25 -0.06435847353417046 1106 26-27 -0.103811408370305 1106 28-29 -0.07683391063545741 1106 30-31 -0.026686361660587465 1106 32-33 -0.08070553661926994 1106 34-35 -0.06565512372539217 1106 36-37 -0.1364411604390412 1106 38-39 -0.10807173463680897 1106 40-41 -0.1071496619978376 1106 42-43 -0.10228041351624029 1106 44-45 -0.15426409917982653 1106 46-47 -0.07004594912848461 1106 48-49 -0.10720124062230951 1106 50-51 -0.10011257763530068 1106 52-53 -0.13409365161016495 1106 54-55 -0.15454228982430607 1106 56-57 -0.12238166852860388 1106 58-59 -0.2501528098350647 1106 60-61 -0.240224175436083 1106 62-63 -0.17161642948845213 1106 64-65 -0.12819804823627834 1106 66-67 -0.06478430563058879 1106 68-69 -0.05863323503470497 1106 70-71 -0.12312631350758352 1106 72-73 -0.1571119545066395 1106 74-75 -0.10421633062973967 1106 76-77 -0.08168303401708954 1106 78-79 -0.056054898183909074 1106 80-81 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36-37 -0.4665760057458854 1107 38-39 -0.5273387810754144 1107 40-41 -0.5728500705650603 1107 42-43 -0.6083109787486123 1107 44-45 -0.5966247320312164 1107 46-47 -0.6404518116545006 1107 48-49 -0.6478827231029101 1107 50-51 -0.6623502758556725 1107 52-53 -0.6196175615788846 1107 54-55 -0.622978935755782 1107 56-57 -0.5689391424739085 1107 58-59 -0.6943454252766159 1107 60-61 -0.7397423849386797 1107 62-63 -0.6878210440462169 1107 64-65 -0.6230458921919393 1107 66-67 -0.621378188535644 1107 68-69 -0.7314111166042778 1107 70-71 -0.7081925925443073 1107 72-73 -0.7098747719360006 1107 74-75 -0.6627475345560043 1107 76-77 -0.6739461224969787 1107 78-79 -0.6352353750339574 1107 80-81 -0.7070807812101094 1107 82-83 -0.7829261656137199 1107 84-85 -0.7051735659035074 1107 86-87 -0.762573141459832 1107 88-89 -0.7964063639908616 1107 90-91 -0.7859907224866305 1107 92-93 -0.7666013183629694 1107 94-95 -0.8206608430517939 1107 96-97 -0.8953199217279 1107 98-99 -0.8577586115726206 1107 100 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0.24766908855297487 2214 38-39 0.26123412755253383 2214 40-41 0.23904858679414787 2214 42-43 0.22248106022777847 2214 44-45 0.28919149920534437 2214 46-47 0.32172562359071577 2214 48-49 0.38004817255544765 2214 50-51 0.3843065455398431 2214 52-53 0.2650507834089737 2214 54-55 0.36538268217488223 2214 56-57 0.45244316128427187 2214 58-59 0.4360349932140579 2214 60-61 0.4512508784232523 2214 62-63 0.39716069872153525 2214 64-65 0.3665404838480981 2214 66-67 0.3534974662144208 2214 68-69 0.39552166396886435 2214 70-71 0.4294473888653556 2214 72-73 0.39916260542450743 2214 74-75 0.4066874089889225 2214 76-77 0.4853191676952875 2214 78-79 0.4683201480317649 2214 80-81 0.4889609594827391 2214 82-83 0.5086616653260343 2214 84-85 0.5145450495776203 2214 86-87 0.5116487916362082 2214 88-89 0.5218694943076017 2214 90-91 0.5286204842446196 2214 92-93 0.5519115486461565 2214 94-95 0.6111310327454973 2214 96-97 0.5968218539684287 2214 98-99 0.6274373274591554 2214 100 0.6294357146735905 2215 1 0.11420857675072327 2215 2 -0.005564354586198306 2215 3 -0.008735697689509436 2215 4 0.020179991905912686 2215 5 0.06901548017650327 2215 6 0.0745392755653782 2215 7 0.07793338038391795 2215 8 -0.003807831969091069 2215 9 0.03380222917360243 2215 10-11 -0.028478330355625303 2215 12-13 -0.01113377314757713 2215 14-15 0.06691623365008326 2215 16-17 0.0753946390745952 2215 18-19 0.11275878246628679 2215 20-21 0.011929280487137817 2215 22-23 0.04467195775718835 2215 24-25 0.12761436384199243 2215 26-27 0.031040967275622222 2215 28-29 0.07272954534548148 2215 30-31 0.04566433291082461 2215 32-33 0.042904330790513256 2215 34-35 -0.01698572307211066 2215 36-37 -0.029581756897876232 2215 38-39 0.05939991937083988 2215 40-41 0.03724275482453976 2215 42-43 0.1369562411482832 2215 44-45 0.16247065163409502 2215 46-47 0.1491973490993388 2215 48-49 0.2171653402165532 2215 50-51 0.2487175283443861 2215 52-53 0.34740478232995997 2215 54-55 0.3126270427656834 2215 56-57 0.29958591806664714 2215 58-59 0.23893737817717664 2215 60-61 0.29020705088289844 2215 62-63 0.25574391461254464 2215 64-65 0.22940165410722102 2215 66-67 0.23344422716126445 2215 68-69 0.17750689657329843 2215 70-71 0.21033138875811375 2215 72-73 0.24020806841511444 2215 74-75 0.22006959101290846 2215 76-77 0.24370369730876007 2215 78-79 0.24857479936429883 2215 80-81 0.26046612163422367 2215 82-83 0.25130805952399626 2215 84-85 0.24021211775983176 2215 86-87 0.20689863447347534 2215 88-89 0.19700401546477053 2215 90-91 0.23252303546782827 2215 92-93 0.25143867651231133 2215 94-95 0.30319685718608014 2215 96-97 0.2782451659260232 2215 98-99 0.28224033036755714 2215 100 0.32314118452502427 2216 1 0.22176370814154467 2216 2 0.12808905811532867 2216 3 0.17844629450988592 2216 4 0.09600383973138804 2216 5 0.1165143203150052 2216 6 0.12996695572383032 2216 7 0.1484618038489387 2216 8 0.09236797854281065 2216 9 0.11623053303075181 2216 10-11 0.11950541453491326 2216 12-13 0.13488251259729367 2216 14-15 0.16019342458106678 2216 16-17 0.16242554855053726 2216 18-19 0.21480901777472639 2216 20-21 0.14917190741571318 2216 22-23 0.15690575537263385 2216 24-25 0.18961927433340264 2216 26-27 0.18569641896216638 2216 28-29 0.16523059616571345 2216 30-31 0.11322188397912925 2216 32-33 0.12696555347708482 2216 34-35 0.17733044933042663 2216 36-37 0.1235174683120448 2216 38-39 0.25722430392163176 2216 40-41 0.2215960350063213 2216 42-43 0.29082365338024374 2216 44-45 0.37101075445654175 2216 46-47 0.4099945930209117 2216 48-49 0.40218300187173384 2216 50-51 0.40302271527269795 2216 52-53 0.3592537335304513 2216 54-55 0.3794214348256162 2216 56-57 0.4108508148552019 2216 58-59 0.4471303576681578 2216 60-61 0.417197592592089 2216 62-63 0.3666808308686029 2216 64-65 0.32206157631234333 2216 66-67 0.3005870666250914 2216 68-69 0.2821776875958051 2216 70-71 0.2866660256537443 2216 72-73 0.24144505496816748 2216 74-75 0.29114178420217485 2216 76-77 0.3241248285010627 2216 78-79 0.32203047006799324 2216 80-81 0.3112374284478534 2216 82-83 0.3229858644091941 2216 84-85 0.32578710141013545 2216 86-87 0.25937898995490727 2216 88-89 0.27505591373897076 2216 90-91 0.3334089870505679 2216 92-93 0.36072769374679936 2216 94-95 0.3128323227347103 2216 96-97 0.3124476359874926 2216 98-99 0.30756450527354673 2216 100 0.33441932203799496 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 97431.0 3 17663.0 4 11721.0 5 10181.0 6 7373.0 7 6873.0 8 7701.0 9 8107.0 10 8541.0 11 9283.0 12 10189.0 13 11616.0 14 12182.0 15 12396.0 16 12135.0 17 12540.0 18 12721.0 19 12223.0 20 11757.0 21 12346.0 22 14294.0 23 20986.0 24 32192.0 25 44438.0 26 50966.0 27 41504.0 28 37354.0 29 46224.0 30 56100.0 31 63181.0 32 79262.0 33 106295.0 34 150525.0 35 230725.0 36 391717.0 37 734256.0 38 1181290.0 39 619503.0 40 2478.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.731242775061844 21.808219908549184 14.440782645293861 22.019754671095104 2 14.580144531002656 22.344532050052155 40.94027038980713 22.135053029138057 3 17.048008149717493 29.02382182992012 31.000613990459456 22.92755602990293 4 11.623112775128423 19.244569679521405 40.278412906460204 28.853904638889965 5 11.684340669347943 40.365555562698006 35.539208858928674 12.410894909025371 6 26.89476929691701 41.15982808236884 19.199165191649918 12.746237429064239 7 24.337492143223884 35.45013054793032 23.694681221193306 16.51769608765249 8 23.908634100779366 37.28587380607705 21.48107795001931 17.32441414312428 9 24.77326139328211 18.528082204157954 22.111032488104307 34.587623914455634 10-11 21.93734026368716 29.364776355785644 29.831297051428923 18.86658632909827 12-13 22.807793734350472 27.539651219793786 29.27276797991819 20.379787065937556 14-15 20.795813291202453 29.155605249365802 26.750989463791953 23.297591995639795 16-17 19.849850224737203 32.16442642821518 27.53320528573815 20.452518061309473 18-19 19.903457243227393 31.008383018804665 29.387411608292137 19.70074812967581 20-21 21.667446846786216 29.96696609967765 28.634802105142754 19.730784948393374 22-23 21.200012460398405 29.98061028843682 28.756602049355067 20.062775201809707 24-25 20.776092400966014 30.152004934697064 28.687206978582385 20.384695685754533 26-27 20.44127633026572 30.135876935164617 28.783777951188842 20.63906878338082 28-29 20.480098961823508 30.021023680354453 29.018600047844867 20.480277309977176 30-31 20.7482209393136 30.40058235046235 28.597276879012455 20.253919831211586 32-33 20.37690047378601 30.219122016157023 28.960539868973147 20.443437641083822 34-35 20.738739692083794 29.988769863044645 28.838254519253237 20.434235925618328 36-37 20.834025510571134 30.056228376274085 28.52898315051332 20.580762962641455 38-39 20.587039912863773 30.18259606031996 28.80663319785679 20.423730828959478 40-41 20.566933923660123 29.890228086965713 28.96645132247445 20.576386666899715 42-43 20.44680005816898 29.946036738965283 28.961599318714278 20.645563884151464 44-45 20.658365185723753 29.964067506851507 28.84004736772131 20.53751993970343 46-47 20.674332886002446 30.108888567045295 28.7138035943905 20.502974952561758 48-49 20.603482063609995 29.94071226147203 28.736716207487344 20.719089467430628 50-51 20.57323905393201 29.86895575435907 28.739113089501416 20.818692102207496 52-53 20.600429329943974 29.897124193311914 28.75938676187884 20.74305971486527 54-55 20.72589548124849 30.155411604833866 28.589844450458685 20.528848463458964 56-57 20.721603173663276 29.89498013012267 28.4655879312522 20.91782876496186 58-59 20.675606320987562 29.723189241973312 28.770655629085525 20.8305488079536 60-61 20.7716550188837 29.971766601687676 28.48348457971785 20.77309379971077 62-63 20.612441176704888 29.918955561994544 28.63019645158456 20.838406809716012 64-65 20.693039769872957 29.77408326517295 28.67176968842175 20.861107276532344 66-67 20.51345313074072 29.775026128644484 28.615259128379332 21.09626161223546 68-69 20.924585636377323 29.97288960477274 28.400175163520235 20.702349595329704 70-71 20.78205233838959 29.743815278677914 28.5059852448653 20.968147138067195 72-73 20.622585071412157 29.82658466099542 28.69917511229095 20.85165515530148 74-75 20.655617559514962 29.908802895022387 28.601329707104412 20.834249838358236 76-77 20.69763446127797 29.836441388871883 28.501670900645472 20.964253249204678 78-79 20.74236166119051 29.836171921389727 28.677725511812636 20.743740905607126 80-81 20.672521535412514 29.78177837089865 28.655336250660397 20.890363843028446 82-83 20.594935634137347 29.772216978204145 28.852777700484992 20.780069687173512 84-85 20.525004664417043 29.765449684882807 28.67250750379545 21.0370381469047 86-87 20.76593695375578 29.7273267774118 28.577357099539817 20.929379169292602 88-89 20.64721232447897 29.659050573718982 28.696020800025686 20.99771630177636 90-91 20.550835516310194 29.691870288961532 28.591531040682444 21.16576315404583 92-93 20.67157108177773 29.765447237072344 28.523288164092648 21.039693517057277 94-95 20.55331452124837 29.616026345955216 28.706656674455694 21.124002458340723 96-97 20.614479128309824 29.737748886732618 28.559343485835676 21.088428499121882 98-99 20.506401903864543 29.657846128381482 28.623962257554897 21.211789710199074 100 20.503971158973364 29.837586090930667 28.476851118522102 21.181591631573866 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 13329.0 1 9717.5 2 5528.0 3 4314.5 4 3081.5 5 2271.0 6 1972.0 7 2035.0 8 2414.0 9 2485.5 10 1968.5 11 1560.5 12 1467.0 13 1484.0 14 1620.5 15 1853.0 16 2169.5 17 2710.5 18 3594.5 19 4688.0 20 6534.0 21 9313.0 22 13502.0 23 20165.5 24 27685.5 25 35506.0 26 46359.5 27 60153.5 28 76983.5 29 93023.0 30 103032.5 31 112940.5 32 123369.0 33 132100.0 34 140507.0 35 147042.5 36 149748.0 37 151026.0 38 151639.0 39 149993.5 40 149342.0 41 146613.5 42 141233.5 43 137108.5 44 134146.0 45 130430.5 46 126821.0 47 122912.0 48 118400.5 49 114680.5 50 112102.5 51 106767.5 52 97292.0 53 89805.0 54 85465.5 55 82491.0 56 75698.5 57 67118.5 58 60852.5 59 54306.0 60 46477.0 61 38705.0 62 32903.5 63 26904.0 64 20688.0 65 16461.0 66 13265.0 67 10189.5 68 7729.0 69 5906.0 70 4490.0 71 3468.5 72 2698.0 73 2127.5 74 1747.5 75 1402.0 76 1017.0 77 823.0 78 695.0 79 533.0 80 393.0 81 271.0 82 200.5 83 167.0 84 147.0 85 134.5 86 116.5 87 97.5 88 89.0 89 82.5 90 72.5 91 62.0 92 55.0 93 45.0 94 35.0 95 41.0 96 38.5 97 28.5 98 33.0 99 41.0 100 50.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17919481858217715 2 0.17515515286689137 3 0.18720286179424367 4 0.1772225112035376 5 0.19086232367750255 6 0.07680117406943329 7 0.07841704035554761 8 0.06665448430221547 9 0.06047617203177838 10-11 0.07358132286695551 12-13 0.0729634916399118 14-15 0.07036147166447772 16-17 0.06657131471395958 18-19 0.07086048919401303 20-21 0.07232189767336641 22-23 0.07000503057195251 24-25 0.06998126783245083 26-27 0.07374766204346728 28-29 0.07154960863956178 30-31 0.07701503872494843 32-33 0.07367637382496223 34-35 0.07286844068190507 36-37 0.0841557419452036 38-39 0.0798190419861468 40-41 0.07462688340502947 42-43 0.07790614145626147 44-45 0.08834986546725031 46-47 0.07908239706159469 48-49 0.08366860578541914 50-51 0.08059133101995143 52-53 0.07947448226337243 54-55 0.08162501018827456 56-57 0.07509025682531226 58-59 0.08276562168435525 60-61 0.07889229514558123 62-63 0.07089613330326555 64-65 0.07398528943848409 66-67 0.07399717080823494 68-69 0.07260705054738659 70-71 0.0685673848321008 72-73 0.06623863636093605 74-75 0.07086048919401303 76-77 0.07607641051463203 78-79 0.0729040847911576 80-81 0.08061509375945311 82-83 0.08264680798684686 84-85 0.08467852221424058 86-87 0.08124480635624767 88-89 0.08237353648257752 90-91 0.08041311047368882 92-93 0.07153772726981093 94-95 0.07113376069828237 96-97 0.07051592947123865 98-99 0.0795576518516283 100 0.0879934243747251 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4208269.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.124827378341784 #Duplication Level Percentage of deduplicated Percentage of total 1 76.63689590231122 48.37690824645344 2 14.582114488502226 18.409869197958393 3 4.465675489081362 8.45684983127859 4 1.7288700787772344 4.365385011295722 5 0.8303864627853227 2.620900106031766 6 0.4437611933909917 1.6807409238007973 7 0.29213132756261234 1.2908517736937009 8 0.17558055921458848 0.8866793993130893 9 0.13631223705398604 0.7744217790229625 >10 0.6356138065998395 7.174286537667597 >50 0.04699595014195349 2.057878740153228 >100 0.024116028696363142 2.772515159378487 >500 0.001093848875096545 0.4570092638809179 >1k 4.149080899062947E-4 0.3481260306449803 >5k 0.0 0.0 >10k+ 3.7718917264190697E-5 0.3275779994262951 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13758 0.3269277700641285 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.3762739501681096E-5 0.0 4 0.0 0.0 0.0 2.3762739501681096E-5 0.0 5 0.0 0.0 0.0 2.3762739501681096E-5 0.0 6 0.0 0.0 0.0 2.3762739501681096E-5 0.0 7 0.0 0.0 0.0 2.3762739501681096E-5 0.0 8 0.0 0.0 0.0 4.752547900336219E-5 0.0 9 0.0 0.0 0.0 4.752547900336219E-5 0.0 10-11 0.0 0.0 0.0 8.316958825588383E-5 0.0 12-13 0.0 0.0 0.0 1.0693232775756493E-4 0.0 14-15 0.0 0.0 0.0 1.1881369750840547E-4 0.0 16-17 0.0 0.0 0.0 1.1881369750840547E-4 0.0 18-19 0.0 0.0 0.0 1.4257643701008656E-4 0.0 20-21 0.0 0.0 0.0 1.9010191601344877E-4 0.0 22-23 0.0 0.0 0.0 3.0891561352185424E-4 0.0 24-25 0.0 0.0 0.0 5.703057480403464E-4 0.0 26-27 0.0 0.0 0.0 9.742723195689249E-4 0.0 28-29 0.0 0.0 0.0 0.0021148838156496174 0.0 30-31 0.0 0.0 0.0 0.005536718303891695 0.0 32-33 0.0 0.0 0.0 0.011394233591056086 0.0 34-35 0.0 0.0 0.0 0.020756752954718436 0.0 36-37 0.0 0.0 0.0 0.03615500815180779 0.0 38-39 0.0 0.0 0.0 0.05849198328338802 0.0 40-41 0.0 0.0 0.0 0.0815774847092712 0.0 42-43 0.0 0.0 0.0 0.10650459844653468 4.752547900336219E-5 44-45 0.0 0.0 0.0 0.135910988579865 4.752547900336219E-5 46-47 0.0 0.0 0.0 0.1735036424715245 4.752547900336219E-5 48-49 0.0 0.0 0.0 0.2152785385154799 4.752547900336219E-5 50-51 0.0 0.0 0.0 0.25801582550925334 7.128821850504328E-5 52-53 0.0 0.0 0.0 0.2985550590991213 7.128821850504328E-5 54-55 0.0 0.0 0.0 0.34598548714447674 7.128821850504328E-5 56-57 0.0 0.0 0.0 0.40649730328550765 7.128821850504328E-5 58-59 0.0 0.0 0.0 0.47766670809304257 7.128821850504328E-5 60-61 0.0 0.0 0.0 0.5530777619016275 7.128821850504328E-5 62-63 0.0 0.0 0.0 0.6321245148539696 7.128821850504328E-5 64-65 0.0 0.0 0.0 0.7145931022945539 7.128821850504328E-5 66-67 0.0 0.0 0.0 0.8106896208393521 7.128821850504328E-5 68-69 0.0 0.0 0.0 0.9248814655146806 7.128821850504328E-5 70-71 0.0 0.0 0.0 1.0504556624113144 7.128821850504328E-5 72-73 0.0 0.0 0.0 1.1824101548641497 7.128821850504328E-5 74-75 0.0 0.0 0.0 1.3164082429141293 7.128821850504328E-5 76-77 0.0 0.0 0.0 1.4695472176327131 7.128821850504328E-5 78-79 0.0 0.0 0.0 1.64661527102949 7.128821850504328E-5 80-81 0.0 0.0 0.0 1.8447846371037593 7.128821850504328E-5 82-83 0.0 0.0 0.0 2.0532551507520074 7.128821850504328E-5 84-85 0.0 0.0 0.0 2.263923717804161 7.128821850504328E-5 86-87 0.0 0.0 0.0 2.4927113737263467 7.128821850504328E-5 88 0.0 0.0 0.0 2.680603354966139 7.128821850504328E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5270 0.0 49.453613 1 GTATCAA 10105 0.0 39.757626 1 ATCAACG 11200 0.0 34.27204 3 TCAACGC 11260 0.0 34.088203 4 CAACGCA 12150 0.0 31.629168 5 TATCAAC 12560 0.0 31.385006 2 AACGCAG 13075 0.0 29.616928 6 GTACATG 20690 0.0 26.989141 1 TACATGG 20685 0.0 26.24328 2 CGCAGAG 14385 0.0 26.13542 8 CATGGGT 9775 0.0 25.744791 4 ACGCAGA 15070 0.0 25.353142 7 ACATGGG 21075 0.0 24.797869 3 GCAGAGT 16130 0.0 22.725298 9 ATGGGTG 5785 0.0 21.38427 5 GTGGTAT 2820 0.0 20.518938 1 TACCTGG 3325 0.0 19.381918 2 ATGGGTA 4180 0.0 19.242516 5 GTACCTG 3570 0.0 18.843723 1 CAGAGTA 15980 0.0 18.65968 10-11 >>END_MODULE