FastQCFastQC Report
Wed 25 May 2016
SRR2431394_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431394_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4208269
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259900.6175935996486916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216080.5134652751523251No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137580.3269277700641285No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50040.1189087484664122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109850.064.765421
GTATCAA220350.044.792331
ATCAACG285500.034.138213
TCAACGC288650.033.7330864
TATCAAC289950.033.7135662
TGGTATC34600.032.886122
CAACGCA298250.032.6157655
GTGGTAT35950.032.5690271
AACGCAG310100.031.4787226
CGCAGAG345850.027.9802078
ACGCAGA347950.027.9329037
GCAGAGT374600.025.7073049
GTACATG204950.025.1688291
TACATGG201850.024.5053182
CATGGGT95700.024.3187244
ACATGGG201400.023.7882373
GAGTACT254950.023.12586812-13
AGAGTAC360350.021.98298310-11
CAGAGTA373100.021.94976810-11
AGTACTT263250.021.41481212-13
Produced by FastQC (version 0.11.5)