##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431394_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4208269
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.65584305566018	34.0	31.0	34.0	31.0	34.0
2	32.76880874297722	34.0	31.0	34.0	31.0	34.0
3	32.845326189937005	34.0	31.0	34.0	31.0	34.0
4	36.30965059505464	37.0	37.0	37.0	35.0	37.0
5	36.21579894251057	37.0	37.0	37.0	35.0	37.0
6	36.26775759819536	37.0	37.0	37.0	35.0	37.0
7	36.227838334479095	37.0	37.0	37.0	35.0	37.0
8	36.24188900471904	37.0	37.0	37.0	35.0	37.0
9	38.036099403341375	39.0	39.0	39.0	35.0	39.0
10-11	38.01598590774496	39.0	39.0	39.0	35.0	39.0
12-13	38.00298887737452	39.0	39.0	39.0	35.0	39.0
14-15	39.47375547998476	41.0	39.0	41.0	36.0	41.0
16-17	39.508147649306636	41.0	39.0	41.0	36.5	41.0
18-19	39.57950097771791	41.0	40.0	41.0	37.0	41.0
20-21	39.5815855640407	41.0	40.0	41.0	37.0	41.0
22-23	39.52751178691286	41.0	40.0	41.0	36.0	41.0
24-25	39.484987057623925	41.0	39.0	41.0	36.0	41.0
26-27	39.37181772362936	41.0	39.0	41.0	36.0	41.0
28-29	39.29535457452934	41.0	39.0	41.0	36.0	41.0
30-31	39.12660668792798	41.0	39.0	41.0	35.0	41.0
32-33	38.91585162450404	41.0	38.5	41.0	35.0	41.0
34-35	38.78934414601348	41.0	38.0	41.0	35.0	41.0
36-37	38.65219119785356	41.0	38.0	41.0	35.0	41.0
38-39	38.510879770280845	41.0	38.0	41.0	35.0	41.0
40-41	38.37774522018435	41.0	38.0	41.0	35.0	41.0
42-43	38.210720963892754	40.5	38.0	41.0	34.0	41.0
44-45	37.97438186579802	40.0	38.0	41.0	33.5	41.0
46-47	37.61786924742691	40.0	38.0	41.0	33.0	41.0
48-49	37.290690899274736	40.0	38.0	41.0	32.5	41.0
50-51	37.12949576179659	40.0	37.5	41.0	32.0	41.0
52-53	37.07196212979731	40.0	37.0	41.0	32.0	41.0
54-55	36.938841480903434	40.0	37.0	41.0	31.5	41.0
56-57	36.794443748724234	40.0	36.5	41.0	31.0	41.0
58-59	36.622484921947716	40.0	36.0	41.0	31.0	41.0
60-61	36.473495753242005	40.0	36.0	41.0	31.0	41.0
62-63	36.32094645090416	39.5	35.0	41.0	31.0	41.0
64-65	36.09196037610714	39.0	35.0	41.0	31.0	41.0
66-67	35.8301524926282	39.0	35.0	41.0	30.0	41.0
68-69	35.54285075407489	38.5	35.0	41.0	30.0	41.0
70-71	35.2434979798107	37.0	35.0	40.5	29.5	41.0
72-73	34.92404204674178	37.0	35.0	40.0	29.0	41.0
74-75	34.583056715243245	36.5	35.0	39.0	29.0	41.0
76-77	34.24465914607645	36.0	35.0	39.0	29.0	41.0
78-79	33.91524365481389	36.0	35.0	38.5	29.0	40.0
80-81	33.63933092204894	35.0	35.0	37.0	29.0	39.0
82-83	33.42716043104659	35.0	35.0	37.0	29.0	39.0
84-85	33.153136004376144	35.0	34.5	36.5	29.0	39.0
86-87	32.902794593216356	35.0	34.0	36.0	29.0	37.0
88-89	32.66273674520332	35.0	34.0	36.0	29.0	37.0
90-91	32.47273510795056	35.0	34.0	36.0	29.0	37.0
92-93	32.30650262613916	35.0	34.0	35.0	29.0	36.0
94-95	32.16628380932873	35.0	34.0	35.0	28.5	36.0
96-97	32.02251982941205	35.0	34.0	35.0	27.0	36.0
98-99	31.908018950309497	35.0	34.0	35.0	27.0	36.0
100	31.806038302209295	35.0	34.0	35.0	27.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.017996554679896803
1101	2	0.008910767344424642
1101	3	0.006576159119795477
1101	4	0.019923695957622556
1101	5	0.053815723020740336
1101	6	0.014715696680418944
1101	7	0.0016442120386557235
1101	8	0.02177649640280066
1101	9	-0.024055122659966344
1101	10-11	0.06875178370479063
1101	12-13	0.06953153250220367
1101	14-15	0.09625645949891748
1101	16-17	0.019494622348261714
1101	18-19	0.014723680988055321
1101	20-21	0.038196792913069544
1101	22-23	0.06399449838882987
1101	24-25	0.03778650307817344
1101	26-27	0.06212855292469044
1101	28-29	0.02699260134895809
1101	30-31	0.033234805838695536
1101	32-33	0.010089343795996797
1101	34-35	-0.004006400122719356
1101	36-37	-0.012914545076306183
1101	38-39	-0.018303357039542334
1101	40-41	-0.018714717139395987
1101	42-43	-0.037144590108084685
1101	44-45	-0.031415922465001245
1101	46-47	-0.0375990940913411
1101	48-49	0.016312081145045454
1101	50-51	-0.05003528406078317
1101	52-53	0.028282052830263638
1101	54-55	0.0037674147031410143
1101	56-57	0.03996362153725386
1101	58-59	0.03247361664288917
1101	60-61	0.011825121094389601
1101	62-63	0.07973041858475227
1101	64-65	0.04752545918769613
1101	66-67	0.02013182312376216
1101	68-69	0.015193614857686555
1101	70-71	-5.697384133185324E-5
1101	72-73	-0.04346283329249445
1101	74-75	-0.0328527985007625
1101	76-77	-0.052400550900081555
1101	78-79	-0.07594522344382426
1101	80-81	0.006171895278939132
1101	82-83	-0.04583681853993937
1101	84-85	0.021073238677878692
1101	86-87	0.019619496913740875
1101	88-89	-0.026129793563548276
1101	90-91	-0.06599938965479168
1101	92-93	-0.027132972740012917
1101	94-95	-0.0617898063595419
1101	96-97	-0.08794582166162002
1101	98-99	-0.05619255904503717
1101	100	-0.04876713936851118
1102	1	-0.02562265157999377
1102	2	-0.0207434450005195
1102	3	-0.017381630424537775
1102	4	0.007822992450030597
1102	5	8.102789160346902E-4
1102	6	-0.04810653714262969
1102	7	-0.051482419328337414
1102	8	-0.005532897785180069
1102	9	-0.03684752577230199
1102	10-11	-0.01645995219398344
1102	12-13	-0.0412684277235158
1102	14-15	-0.09484218071784767
1102	16-17	-0.06685099129431649
1102	18-19	-0.04088542483638946
1102	20-21	-0.015898404342507888
1102	22-23	0.013820796646214717
1102	24-25	0.004488593713816158
1102	26-27	0.03697163095685596
1102	28-29	8.22988899606969E-4
1102	30-31	-8.601428276193701E-4
1102	32-33	0.0019440060874913456
1102	34-35	4.977264589314245E-4
1102	36-37	-0.047240992487253664
1102	38-39	-0.010598333050680253
1102	40-41	0.022228865528951758
1102	42-43	-0.08527799553230153
1102	44-45	-0.1313217692843054
1102	46-47	-0.13177558003362577
1102	48-49	-0.06954536534611577
1102	50-51	-0.0824855040622765
1102	52-53	-0.1765993079473276
1102	54-55	-0.14727324206967296
1102	56-57	-0.16684554892039216
1102	58-59	-0.14941075206469634
1102	60-61	-0.21893158460079576
1102	62-63	-0.17005237655284589
1102	64-65	-0.23535730611919092
1102	66-67	-0.19111497628956187
1102	68-69	-0.2042494830616164
1102	70-71	-0.22225350850710157
1102	72-73	-0.1644269867217858
1102	74-75	-0.23809927474805903
1102	76-77	-0.23196247448374407
1102	78-79	-0.20714068917578743
1102	80-81	-0.26096180845239303
1102	82-83	-0.21062477190459106
1102	84-85	-0.18510171844047107
1102	86-87	-0.18132203489325605
1102	88-89	-0.23176655494272325
1102	90-91	-0.2391607613703144
1102	92-93	-0.28246396933434426
1102	94-95	-0.23304605216459606
1102	96-97	-0.19001998826613686
1102	98-99	-0.21159720020644457
1102	100	-0.18400839524143464
1103	1	-0.0618000013186375
1103	2	-0.09972914798323984
1103	3	-0.06136456482546038
1103	4	-0.023142550994521116
1103	5	-0.05382788940939065
1103	6	-0.05254638105812148
1103	7	-0.07466624706992064
1103	8	-0.030726394032043913
1103	9	-0.0799280069009285
1103	10-11	-0.04417836567699851
1103	12-13	-0.05397932819332851
1103	14-15	-0.05962785377465707
1103	16-17	-0.06879503375431284
1103	18-19	-0.11365750487428272
1103	20-21	-0.14274069109102072
1103	22-23	-0.08031503502226656
1103	24-25	-0.1447306248980169
1103	26-27	-0.1159030453713541
1103	28-29	-0.07647008407829503
1103	30-31	-0.0966933347958161
1103	32-33	-0.060370588101051226
1103	34-35	-0.05979741240730618
1103	36-37	-0.05637067912206817
1103	38-39	-0.06609828498653769
1103	40-41	-0.0801458893112752
1103	42-43	-0.02463036813947639
1103	44-45	-0.05366998227675168
1103	46-47	-0.09537799972626715
1103	48-49	-0.08660305341285124
1103	50-51	-0.14030324612614464
1103	52-53	-0.08752217325766765
1103	54-55	-0.061897807806850835
1103	56-57	-0.05941323889665284
1103	58-59	-0.056369521509466836
1103	60-61	-0.05886634350363806
1103	62-63	-0.08286594617038645
1103	64-65	-0.07349362865091535
1103	66-67	-0.027843193335947092
1103	68-69	0.06074987427073353
1103	70-71	-0.05290166547546704
1103	72-73	-0.009460296697248793
1103	74-75	0.014357542552936309
1103	76-77	0.03839853618562472
1103	78-79	0.0709148529682011
1103	80-81	0.07856753155512308
1103	82-83	0.025198099321343648
1103	84-85	-0.010107884135202028
1103	86-87	0.027691264738628263
1103	88-89	0.028001449647071297
1103	90-91	0.061251486548471235
1103	92-93	-0.00440831844793621
1103	94-95	-0.06389706810144702
1103	96-97	-0.0033605127351421515
1103	98-99	0.01808702596732914
1103	100	0.05038424141841702
1104	1	-0.10889073023363238
1104	2	-0.04326314569905776
1104	3	-0.045105547196854445
1104	4	-0.012233810194956618
1104	5	-0.05266499888246301
1104	6	-0.0678109645172782
1104	7	-0.05102190773165205
1104	8	-0.07748519350695915
1104	9	-0.05079835481747352
1104	10-11	-0.029033286024493066
1104	12-13	-0.036210879310750954
1104	14-15	-0.09127430342150689
1104	16-17	-0.05753573039989135
1104	18-19	-0.06312701630481143
1104	20-21	-0.024313461393738578
1104	22-23	-0.07144438594259128
1104	24-25	-0.02644647477252704
1104	26-27	-0.011128214917803803
1104	28-29	0.002546333916633614
1104	30-31	0.036844321835417304
1104	32-33	0.01615989342684543
1104	34-35	-0.00531895139425842
1104	36-37	0.0215399258014628
1104	38-39	-0.030936663028057865
1104	40-41	-0.037008400486406856
1104	42-43	-0.04075410610479935
1104	44-45	-0.023704683744732336
1104	46-47	-0.011676206478547613
1104	48-49	-0.0924656054833406
1104	50-51	-0.2230459485398626
1104	52-53	-0.17573763543880716
1104	54-55	-0.13511691671605064
1104	56-57	-0.1672554104561783
1104	58-59	-0.0978791315113341
1104	60-61	-0.08103788136664747
1104	62-63	-0.13355916303953563
1104	64-65	-0.1471423012716926
1104	66-67	-0.18298548614612997
1104	68-69	-0.19497865749669785
1104	70-71	-0.1868494266715217
1104	72-73	-0.21696570449840635
1104	74-75	-0.20307429152783385
1104	76-77	-0.2708583031560323
1104	78-79	-0.2593742636407015
1104	80-81	-0.2556820833921023
1104	82-83	-0.2794709448729975
1104	84-85	-0.30690051029669263
1104	86-87	-0.2763608148171883
1104	88-89	-0.2601740921689668
1104	90-91	-0.27715607546283394
1104	92-93	-0.2713495436998201
1104	94-95	-0.21503016731114144
1104	96-97	-0.22838880771576342
1104	98-99	-0.24347721860204885
1104	100	-0.17313137234636145
1105	1	-0.17167474220256906
1105	2	-0.1655756551907146
1105	3	-0.18379006470229342
1105	4	-0.07699527031540754
1105	5	-0.1211347877216653
1105	6	-0.03792853839305366
1105	7	-0.03670493906722072
1105	8	-0.041145455473412085
1105	9	-0.06904621293566748
1105	10-11	-0.08924259657033673
1105	12-13	-0.0873384385768361
1105	14-15	-0.15377633438768612
1105	16-17	-0.13261144813505155
1105	18-19	-0.20518940360690863
1105	20-21	-0.15523441585831677
1105	22-23	-0.17297837055566134
1105	24-25	-0.1974906997657797
1105	26-27	-0.15470210528077644
1105	28-29	-0.12348949199524384
1105	30-31	-0.13257864502650563
1105	32-33	-0.15034604229979465
1105	34-35	-0.13566374147690397
1105	36-37	-0.125035616524535
1105	38-39	-0.06609880542050917
1105	40-41	-0.14162321987465276
1105	42-43	-0.23117331742011515
1105	44-45	-0.18421865579731644
1105	46-47	-0.09345255416139508
1105	48-49	-0.11061929308791463
1105	50-51	-0.1277520867994184
1105	52-53	-0.08879596876661822
1105	54-55	-0.01937273423216368
1105	56-57	-0.09161997502034325
1105	58-59	-0.12833148819475326
1105	60-61	-0.09308912073682052
1105	62-63	-0.13020735592727561
1105	64-65	-0.1464201062763948
1105	66-67	-0.1496361924581393
1105	68-69	-0.02555529273119106
1105	70-71	-0.016351921195372654
1105	72-73	-0.03792355125645486
1105	74-75	-0.06258044013288355
1105	76-77	-0.09741127108566872
1105	78-79	0.01049952797586684
1105	80-81	0.010366661122631626
1105	82-83	-0.07321848698943256
1105	84-85	-0.04886461541485687
1105	86-87	-0.06424027390706044
1105	88-89	-0.02494967786645219
1105	90-91	-0.01069218488100887
1105	92-93	-0.024375368305250333
1105	94-95	-0.04841865450163141
1105	96-97	-0.03765240093097333
1105	98-99	-0.028189829426487734
1105	100	-0.02640840674446565
1106	1	-0.16358372682683608
1106	2	-0.09823528985661056
1106	3	-0.10261450146834505
1106	4	-0.05938419299186393
1106	5	-0.08562425894372439
1106	6	-0.09694225551650959
1106	7	-0.0628722337354759
1106	8	-0.0664659736695441
1106	9	-0.08253147078874434
1106	10-11	-0.035472755044509086
1106	12-13	-0.07662350502338455
1106	14-15	-0.12194660104344734
1106	16-17	-0.15745630125286425
1106	18-19	-0.10918449226724647
1106	20-21	-0.11525945507240465
1106	22-23	-0.12112147183159294
1106	24-25	-0.05949406653891742
1106	26-27	-0.06573379624568076
1106	28-29	-0.12373997444650797
1106	30-31	-0.10266637000594159
1106	32-33	-0.024566966402645107
1106	34-35	-0.09583870891999169
1106	36-37	-0.06494786316182655
1106	38-39	-0.029493623820414427
1106	40-41	-0.09276662743199182
1106	42-43	-0.11350567995924621
1106	44-45	-0.06710237646351658
1106	46-47	-0.094343737566156
1106	48-49	0.012348780107920732
1106	50-51	0.014425455265246967
1106	52-53	0.028474780121079846
1106	54-55	-4.109223880277568E-4
1106	56-57	0.012249827259140034
1106	58-59	0.04927042648975544
1106	60-61	0.03738373697702002
1106	62-63	-0.0025044612713074343
1106	64-65	-0.021545928069130582
1106	66-67	0.030779950300470205
1106	68-69	-0.0029137562771310854
1106	70-71	0.0034875403892797863
1106	72-73	0.032988630615349734
1106	74-75	-0.03025854070310885
1106	76-77	-0.017760186754038898
1106	78-79	0.010059483914687917
1106	80-81	0.03254355037184098
1106	82-83	0.056904056534392566
1106	84-85	0.0434918961354569
1106	86-87	0.0469929254485848
1106	88-89	0.013515614929019648
1106	90-91	-9.830128754941825E-4
1106	92-93	0.020378356419762156
1106	94-95	-0.03610658084753737
1106	96-97	-0.03869220951413155
1106	98-99	-0.08002369898879991
1106	100	-0.047083665632236205
1107	1	-0.28965014119952315
1107	2	-0.2287669582967098
1107	3	-0.16558656516620118
1107	4	-0.23558650113394464
1107	5	-0.2490428202845152
1107	6	-0.225022555935098
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2216	24-25	-0.05267477408753507
2216	26-27	-0.03324062049026111
2216	28-29	0.00751637270848704
2216	30-31	-0.04498606996177301
2216	32-33	-0.010375182207035039
2216	34-35	0.0059500528607259184
2216	36-37	0.01255445004623823
2216	38-39	0.04582895409095045
2216	40-41	6.920735079347651E-4
2216	42-43	0.007496901480891438
2216	44-45	0.02576608118062751
2216	46-47	0.07838828215498239
2216	48-49	0.07236334244446851
2216	50-51	0.0796840720291101
2216	52-53	0.05406664179460563
2216	54-55	0.09361666130354251
2216	56-57	0.1111193521136471
2216	58-59	0.05933525553698615
2216	60-61	-0.007743639051668083
2216	62-63	0.03700626586735467
2216	64-65	0.029359944834816076
2216	66-67	0.05846820847470724
2216	68-69	0.04369289869638493
2216	70-71	0.06022040023735542
2216	72-73	0.04643265281806919
2216	74-75	0.07546653439064244
2216	76-77	0.08758943491314852
2216	78-79	0.08645124282888617
2216	80-81	0.10456495420885403
2216	82-83	0.16591882900478083
2216	84-85	0.1647671614626205
2216	86-87	0.12127299240551537
2216	88-89	0.14721819365313848
2216	90-91	0.1477904814256732
2216	92-93	0.11671567657817405
2216	94-95	0.04258211045972615
2216	96-97	0.0399868838430848
2216	98-99	0.048746146049232664
2216	100	0.0817179241150292
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	46.0
3	234.0
4	62.0
5	100.0
6	253.0
7	662.0
8	1316.0
9	2450.0
10	3522.0
11	4902.0
12	7609.0
13	11515.0
14	13305.0
15	17582.0
16	22737.0
17	35037.0
18	40527.0
19	20723.0
20	12365.0
21	11021.0
22	10724.0
23	10929.0
24	11958.0
25	13833.0
26	17072.0
27	21531.0
28	27113.0
29	34131.0
30	43190.0
31	54972.0
32	69944.0
33	90572.0
34	124828.0
35	199778.0
36	329076.0
37	697919.0
38	1330745.0
39	903862.0
40	10124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.18551210801804	21.397881863234126	14.954190052466258	22.462415976281584
2	14.18521052440218	22.15360566452167	40.81153740405082	22.849646407025332
3	16.377246455229155	28.487627015658084	32.275908152598674	22.859218376514082
4	10.987391728047804	18.84839110807793	40.42557640683141	29.738640757042862
5	11.044646253328262	39.38362970711297	35.98350133130468	13.588222708254088
6	26.795364079625138	40.659140373393434	18.762037312728822	13.78345823425261
7	23.700552412405195	35.39072716121522	23.989032069955606	16.919688356423983
8	24.507939012453814	35.48929500466819	21.473342127131133	18.529423855746867
9	25.391812209589414	16.156203053704303	22.652916902115976	35.7990678345903
10-11	22.34023062689196	27.869190396336357	29.976291914799173	19.814287061972514
12-13	24.019876105828786	26.21573145633038	30.00254974194853	19.7618426958923
14-15	21.34158961796406	27.375067991138398	28.71495382068019	22.56838857021735
16-17	19.001853137460216	30.67129628254471	29.362218689230502	20.964631890764572
18-19	18.921841095377395	28.336014366010325	33.31516670819208	19.426977830420196
20-21	20.539110023622538	27.355879578990795	33.098228749160285	19.006781648226383
22-23	19.980355329249626	27.388727328548146	33.3101923690185	19.32072497318373
24-25	19.687049287396604	27.562928821166626	33.20385008886676	19.546171802570015
26-27	19.408383124252662	27.60179732070041	33.222559231074854	19.767260323972067
28-29	19.409183438145046	27.51890003007181	33.447869969076294	19.624046562706848
30-31	19.71840624904206	27.867865134790833	33.01619124859413	19.39753736757297
32-33	19.40479283914999	27.723722798891835	33.33549577011919	19.535988591838986
34-35	19.742005852810028	27.5775235936206	33.02024482948314	19.660225724086224
36-37	19.89970570661127	27.67180055298974	32.6198361276888	19.808657612710185
38-39	19.698305284113086	27.87907735501467	32.72793593026993	19.694681430602316
40-41	19.718515524407294	27.693580280973574	32.67988043991098	19.90802375470815
42-43	19.713605315845772	27.76743103455036	32.475304155636245	20.04365949396762
44-45	19.976700597930858	27.935733571753186	32.07599335830405	20.011572472011903
46-47	20.174261922318067	28.229426250212974	31.424414301102377	20.171897526366582
48-49	20.288750964471564	28.28690839902354	30.940426014248633	20.48391462225626
50-51	20.2883070459227	28.353238449224754	30.66748664203335	20.6909678628192
52-53	20.29638469414937	28.51948804335867	30.61964993974926	20.5644773227427
54-55	20.420646060907632	28.815745105853015	30.439998564720984	20.323610268518365
56-57	20.309537210277444	28.449351864360544	30.44133867197632	20.799772253385694
58-59	20.25266854401501	28.21217085858092	30.823559157324777	20.711601440079292
60-61	20.35565927865343	28.415909555908886	30.59860563141647	20.629825534021208
62-63	20.206391644149193	28.337075618558284	30.785822865905317	20.670709871387213
64-65	20.25137570597236	28.238466632907205	30.8188435776506	20.691314083469834
66-67	20.2045696683549	28.242034661213566	30.687398807366055	20.86599686306548
68-69	20.587985085497472	28.47535545034959	30.32392539136787	20.61273407278507
70-71	20.493185417708766	28.29462568214141	30.344548987644522	20.8676399125053
72-73	20.34214148891501	28.38801033871727	30.469025314268915	20.8008228580988
74-75	20.442308370921292	28.485539265163197	30.2562674352856	20.81588492862991
76-77	20.521387666054018	28.408370594631023	30.14797308560615	20.92226865370881
78-79	20.54619162925981	28.449977781632658	30.28611791655874	20.717712672548792
80-81	20.442183320691445	28.434815380901053	30.273449752770286	20.84955154563722
82-83	20.35008504811789	28.436167335914643	30.459689948638445	20.754057667329022
84-85	20.243751833467613	28.4533737526925	30.252367126247957	21.050507287591923
86-87	20.38080201588766	28.434027514566306	30.18067706849365	21.004493401052382
88-89	20.245888248276437	28.307755371193828	30.2592015018253	21.187154878704437
90-91	20.155058746453214	28.377007667033844	30.18376096353522	21.284172622977717
92-93	20.203886921176796	28.37583155803589	30.178436700596333	21.241844820190984
94-95	20.03176155687234	28.24593558530043	30.34717430027622	21.375128557551008
96-97	20.069178169430437	28.384602296814077	30.16677423279528	21.379445300960207
98-99	19.972378063610535	28.2825317272657	30.171440642956178	21.573649566167596
100	19.97863250402007	28.368154921949944	30.046092673434465	21.60711990059552
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2947.0
1	1685.5
2	1527.5
3	2704.5
4	1789.0
5	1097.0
6	2573.5
7	6746.5
8	11941.5
9	13388.0
10	9208.0
11	5023.0
12	4436.5
13	4788.0
14	5212.5
15	5765.5
16	6374.5
17	7201.0
18	8179.5
19	9265.0
20	10880.0
21	13370.0
22	17378.5
23	23517.5
24	30436.0
25	37891.0
26	48119.5
27	61264.0
28	77546.0
29	92619.5
30	102094.5
31	111853.0
32	121735.5
33	130224.5
34	138687.5
35	144764.5
36	147604.0
37	149443.0
38	150083.0
39	148341.5
40	147510.0
41	145061.0
42	139966.5
43	136116.5
44	133827.0
45	130662.5
46	126455.0
47	122007.5
48	117365.5
49	113516.5
50	111401.0
51	105906.5
52	96190.0
53	88235.0
54	83016.0
55	79199.0
56	72065.5
57	63754.0
58	58186.0
59	52555.5
60	44772.5
61	36743.0
62	31032.0
63	25275.0
64	19176.5
65	14891.5
66	11741.5
67	8799.5
68	6538.5
69	4800.0
70	3412.0
71	2547.5
72	1942.5
73	1477.5
74	1204.5
75	933.5
76	582.0
77	453.0
78	357.5
79	185.5
80	76.0
81	40.0
82	24.0
83	14.5
84	7.5
85	4.5
86	2.5
87	1.0
88	0.5
89	0.5
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005489192824888333
2	0.004324818589305959
3	6.653567060470707E-4
4	0.0
5	0.04441256012864197
6	0.0
7	0.0
8	0.0
9	7.128821850504328E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.1881369750840548E-5
18-19	5.940684875420274E-5
20-21	0.0
22-23	7.128821850504328E-5
24-25	5.940684875420274E-5
26-27	1.1881369750840547E-4
28-29	2.019832857642893E-4
30-31	1.9010191601344877E-4
32-33	0.0012713065633399385
34-35	8.079331430571572E-4
36-37	0.0012713065633399385
38-39	9.861536893197654E-4
40-41	2.019832857642893E-4
42-43	4.8713615978446246E-4
44-45	1.5445780676092712E-4
46-47	1.4257643701008656E-4
48-49	1.4257643701008656E-4
50-51	2.970342437710137E-4
52-53	5.227802690369841E-4
54-55	6.653567060470707E-4
56-57	0.0019841887483903714
58-59	6.772380757979112E-4
60-61	0.0013307134120941414
62-63	0.0013307134120941414
64-65	0.0015683408071109522
66-67	4.8713615978446246E-4
68-69	3.8020383202689754E-4
70-71	3.3267835302353536E-4
72-73	2.3762739501681095E-4
74-75	6.772380757979112E-4
76-77	8.91102731313041E-4
78-79	9.267468405655627E-4
80-81	8.792213615622006E-4
82-83	0.0014970525886059088
84-85	0.0012950693028416197
86-87	0.0023287484711647473
88-89	0.0013425947818449819
90-91	0.001366357521346663
92-93	0.001283187933090779
94-95	8.792213615622006E-4
96-97	1.1881369750840547E-4
98-99	4.6337342028278134E-4
100	1.9010191601344877E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4208269.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.57069017660253
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.92038101524275	43.295677341801955
2	16.067322497438482	18.821483359300505
3	5.035068066540497	8.84722235230346
4	1.9662236925098793	4.606523148475662
5	0.9455964136289616	2.76921172873842
6	0.5382740050596819	1.891624798828177
7	0.32991004714183986	1.3526141410105128
8	0.2103306471003646	0.9855368932767823
9	0.14787298249436961	0.7794920378851106
>10	0.7497419762471141	7.862543620565235
>50	0.054087115542394176	2.217210874252176
>100	0.032301615803860415	3.3873445898818466
>500	0.0017909397662852497	0.6849269434767328
>1k	9.361728192213809E-4	0.9165574565544793
>5k	4.070316605309763E-5	0.11929598690626347
>10k+	1.221094981592929E-4	1.4627347267427462
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25990	0.6175935996486916	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21608	0.5134652751523251	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13758	0.3269277700641285	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5004	0.1189087484664122	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	1.1881369750840548E-5	0.0
16-17	0.0	0.0	0.0	2.3762739501681096E-5	0.0
18-19	0.0	0.0	0.0	4.752547900336219E-5	0.0
20-21	0.0	0.0	0.0	7.128821850504328E-5	0.0
22-23	0.0	0.0	0.0	1.782205462626082E-4	0.0
24-25	0.0	0.0	0.0	4.277293110302597E-4	1.1881369750840548E-5
26-27	0.0	0.0	0.0	8.316958825588383E-4	2.3762739501681096E-5
28-29	0.0	0.0	0.0	0.0019604260088886905	2.3762739501681096E-5
30-31	0.0	0.0	0.0	0.005394141866881608	2.3762739501681096E-5
32-33	0.0	0.0	0.0	0.011275419893547679	3.564410925252165E-5
34-35	0.0	0.0	0.0	0.020839922542974322	4.752547900336219E-5
36-37	0.0	0.0	0.0	0.03644016102582796	4.752547900336219E-5
38-39	0.0	0.0	0.0	0.058955356703670796	4.752547900336219E-5
40-41	0.0	0.0	0.0	0.08211214634805902	5.940684875420274E-5
42-43	0.0	0.0	0.0	0.10737193843834603	7.128821850504328E-5
44-45	0.0	0.0	0.0	0.13714665103395246	7.128821850504328E-5
46-47	0.0	0.0	0.0	0.1751432714971405	7.128821850504328E-5
48-49	0.0	0.0	0.0	0.2176072869866446	7.128821850504328E-5
50-51	0.0	0.0	0.0	0.2611406257537244	7.128821850504328E-5
52-53	0.0	0.0	0.0	0.3022739278311344	9.505095800672438E-5
54-55	0.0	0.0	0.0	0.35070239093556044	9.505095800672438E-5
56-57	0.0	0.0	0.0	0.41203402158939934	9.505095800672438E-5
58-59	0.0	0.0	0.0	0.4845103770695267	9.505095800672438E-5
60-61	0.0	0.0	0.0	0.5611095678531957	9.505095800672438E-5
62-63	0.0	0.0	0.0	0.6415702038058879	9.505095800672438E-5
64-65	0.0	0.0	0.0	0.7254407928770713	9.505095800672438E-5
66-67	0.0	0.0	0.0	0.8234739746912567	9.505095800672438E-5
68-69	0.0	0.0	0.0	0.9389965327786793	9.505095800672438E-5
70-71	0.0	0.0	0.0	1.0670895800624913	9.505095800672438E-5
72-73	0.0	0.0	0.0	1.2012183631797302	9.505095800672438E-5
74-75	0.0	0.0	0.0	1.3374620301126188	9.505095800672438E-5
76-77	0.0	0.0	0.0	1.4932386689158892	9.505095800672438E-5
78-79	0.0	0.0	0.0	1.6736810313219046	9.505095800672438E-5
80-81	0.0	0.0	0.0	1.8754860965399311	9.505095800672438E-5
82-83	0.0	0.0	0.0	2.08834083562624	9.505095800672438E-5
84-85	0.0	0.0	0.0	2.3042134426292615	9.505095800672438E-5
86-87	0.0	0.0	0.0	2.5381219689140595	9.505095800672438E-5
88	0.0	0.0	0.0	2.7306476843566796	9.505095800672438E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10985	0.0	64.76542	1
GTATCAA	22035	0.0	44.79233	1
ATCAACG	28550	0.0	34.13821	3
TCAACGC	28865	0.0	33.733086	4
TATCAAC	28995	0.0	33.713566	2
TGGTATC	3460	0.0	32.88612	2
CAACGCA	29825	0.0	32.615765	5
GTGGTAT	3595	0.0	32.569027	1
AACGCAG	31010	0.0	31.478722	6
CGCAGAG	34585	0.0	27.980207	8
ACGCAGA	34795	0.0	27.932903	7
GCAGAGT	37460	0.0	25.707304	9
GTACATG	20495	0.0	25.168829	1
TACATGG	20185	0.0	24.505318	2
CATGGGT	9570	0.0	24.318724	4
ACATGGG	20140	0.0	23.788237	3
GAGTACT	25495	0.0	23.125868	12-13
AGAGTAC	36035	0.0	21.982983	10-11
CAGAGTA	37310	0.0	21.949768	10-11
AGTACTT	26325	0.0	21.414812	12-13
>>END_MODULE