FastQCFastQC Report
Wed 25 May 2016
SRR2431393_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431393_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3752017
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA199980.5329933206592614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52750.049.2435531
GTATCAA91500.038.0577741
TCAACGC106850.031.2654274
ATCAACG107750.031.0496043
TATCAAC117550.029.341692
CAACGCA116550.028.742925
GTACATG209600.027.3456821
TACATGG208650.026.5888272
AACGCAG126650.026.3468956
CATGGGT104300.025.3080754
ACATGGG213850.025.0181393
TACCTGG28450.024.8091982
CGCAGAG139050.023.252868
ACGCAGA142950.023.1447877
TATACCG2704.5474735E-1122.6538875
ATGGGTG55300.021.781015
GTGGTAT25900.021.6212081
GTACCTG34450.021.0360551
TGGTATC26550.020.3815442
GCAGAGT156050.020.2983559
Produced by FastQC (version 0.11.5)