##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431393_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3752017 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.746247684911875 33.0 31.0 34.0 26.0 34.0 2 30.879106891040205 33.0 31.0 34.0 27.0 34.0 3 30.913967607289624 34.0 31.0 34.0 27.0 34.0 4 34.0898599340035 37.0 35.0 37.0 30.0 37.0 5 34.217094698664745 37.0 35.0 37.0 30.0 37.0 6 34.17374201662732 37.0 35.0 37.0 30.0 37.0 7 34.13941701223635 37.0 35.0 37.0 30.0 37.0 8 34.101706895251276 37.0 35.0 37.0 30.0 37.0 9 35.51600832299001 39.0 37.0 39.0 29.0 39.0 10-11 35.55349322777589 39.0 37.0 39.0 30.0 39.0 12-13 35.49491926609075 39.0 37.0 39.0 29.5 39.0 14-15 36.838793774122024 40.5 38.0 41.0 29.5 41.0 16-17 36.86354526112221 40.5 38.0 41.0 30.0 41.0 18-19 36.8937286264961 40.0 38.0 41.0 30.0 41.0 20-21 36.99092781296034 40.5 38.0 41.0 30.0 41.0 22-23 36.97011740618446 40.0 38.0 41.0 30.0 41.0 24-25 37.023202986553635 40.0 38.0 41.0 30.0 41.0 26-27 37.07547247253944 40.0 38.0 41.0 30.0 41.0 28-29 37.04557348754017 40.0 38.0 41.0 30.0 41.0 30-31 36.97415230261483 40.0 38.0 41.0 30.0 41.0 32-33 36.91967040661063 40.0 38.0 41.0 30.0 41.0 34-35 36.84160439571569 40.0 37.5 41.0 30.0 41.0 36-37 36.700368095347116 40.0 37.0 41.0 30.0 41.0 38-39 36.57375233107952 40.0 37.0 41.0 29.0 41.0 40-41 36.505343925680506 40.0 37.0 41.0 29.0 41.0 42-43 36.32855075016984 40.0 36.5 41.0 28.0 41.0 44-45 36.164021378367956 40.0 36.0 41.0 28.0 41.0 46-47 36.02741818600502 40.0 36.0 41.0 28.0 41.0 48-49 35.88467083171531 40.0 35.5 41.0 27.0 41.0 50-51 35.746971562229064 40.0 35.0 41.0 27.0 41.0 52-53 35.58038023281877 40.0 35.0 41.0 27.0 41.0 54-55 35.41927661841618 39.0 35.0 41.0 27.0 41.0 56-57 35.2295410708427 39.0 35.0 41.0 27.0 41.0 58-59 35.01761399268713 39.0 34.5 41.0 26.0 41.0 60-61 34.78655680398037 38.5 34.0 41.0 26.0 41.0 62-63 34.692944488257915 38.0 34.0 40.5 26.0 41.0 64-65 34.451002354200426 38.0 34.0 40.0 26.0 41.0 66-67 34.19632866802043 37.5 34.0 40.0 25.5 41.0 68-69 33.9058581024553 37.0 34.0 40.0 25.0 41.0 70-71 33.57926896386664 36.5 33.0 39.5 24.5 41.0 72-73 33.23670961512168 36.0 33.0 39.0 23.5 41.0 74-75 32.94599371484724 36.0 33.0 39.0 23.0 41.0 76-77 32.67371989519238 35.0 33.0 38.5 22.5 40.0 78-79 32.319370621188554 35.0 33.0 37.0 21.5 39.5 80-81 31.982052586648727 35.0 32.5 37.0 20.0 39.0 82-83 31.648316758692722 35.0 32.0 36.5 19.5 39.0 84-85 31.340480333644543 35.0 32.0 36.0 18.5 37.5 86-87 31.05034825268649 35.0 32.0 36.0 17.5 37.0 88-89 30.77768304354698 35.0 32.0 35.5 13.0 37.0 90-91 30.540468100224494 35.0 31.5 35.0 7.5 36.0 92-93 30.304892541798186 35.0 31.0 35.0 2.0 36.0 94-95 30.091461872374246 35.0 31.0 35.0 2.0 36.0 96-97 29.893031268248517 35.0 31.0 35.0 2.0 36.0 98-99 29.675812769505043 35.0 31.0 35.0 2.0 35.0 100 29.50612217375348 35.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30925243216616494 1101 2 0.3008846654683204 1101 3 0.38637408150628616 1101 4 0.4261566966772392 1101 5 0.43195233878672923 1101 6 0.44377112879180913 1101 7 0.3947808351598354 1101 8 0.39344486299059156 1101 9 0.44990516955866866 1101 10-11 0.4995214842479285 1101 12-13 0.39434057596996297 1101 14-15 0.4632558613882196 1101 16-17 0.39406969384286583 1101 18-19 0.41002016813669684 1101 20-21 0.3801178727361716 1101 22-23 0.38835032244892886 1101 24-25 0.36617356136697765 1101 26-27 0.31196098337587586 1101 28-29 0.32432381129162735 1101 30-31 0.36061604804979197 1101 32-33 0.38119875701417527 1101 34-35 0.32571641480216584 1101 36-37 0.30592662968928863 1101 38-39 0.3378339831266004 1101 40-41 0.3068665075829742 1101 42-43 0.28851668748138337 1101 44-45 0.30650157645725074 1101 46-47 0.23892060518807057 1101 48-49 0.24879344545706772 1101 50-51 0.341479150673635 1101 52-53 0.29627949729337644 1101 54-55 0.3167176872515398 1101 56-57 0.26144151316125175 1101 58-59 0.24571650291082392 1101 60-61 0.23738198818610812 1101 62-63 0.26276123026949705 1101 64-65 0.3397469985363841 1101 66-67 0.3448377256470607 1101 68-69 0.34711781912982076 1101 70-71 0.3954054068747723 1101 72-73 0.31000171188209436 1101 74-75 0.376398667474767 1101 76-77 0.43372869980673556 1101 78-79 0.42347169549197616 1101 80-81 0.41237174524707143 1101 82-83 0.36998883657807724 1101 84-85 0.3691628647633891 1101 86-87 0.3083993264020144 1101 88-89 0.2983071463017737 1101 90-91 0.3165211154013363 1101 92-93 0.25400073888178554 1101 94-95 0.3021319791995509 1101 96-97 0.32729548993280844 1101 98-99 0.2473450572699285 1101 100 0.229961437655696 1102 1 0.19122283755609004 1102 2 0.2085761053852906 1102 3 0.22078796868854766 1102 4 0.2702223282614824 1102 5 0.33503988343376534 1102 6 0.3197760994089336 1102 7 0.25892383759307336 1102 8 0.28830936854956235 1102 9 0.3188709202016895 1102 10-11 0.2737451724319513 1102 12-13 0.30418548716183125 1102 14-15 0.36458668164418384 1102 16-17 0.3528964442324991 1102 18-19 0.34439873686275746 1102 20-21 0.3890026772958137 1102 22-23 0.36113315646433364 1102 24-25 0.33374418960161023 1102 26-27 0.35132899995385714 1102 28-29 0.2737288645209688 1102 30-31 0.324875679488251 1102 32-33 0.25212576669507314 1102 34-35 0.3165111408107819 1102 36-37 0.3146488131508747 1102 38-39 0.32318897266122804 1102 40-41 0.29136448219533406 1102 42-43 0.3062323209747433 1102 44-45 0.2729048733093009 1102 46-47 0.16258535927789097 1102 48-49 0.13415112509524363 1102 50-51 0.12321516994519044 1102 52-53 0.22447768329580953 1102 54-55 0.07760375529206698 1102 56-57 0.07990655743514452 1102 58-59 0.035255905117750785 1102 60-61 0.022839114877832856 1102 62-63 0.1454415626621639 1102 64-65 0.17674850499436445 1102 66-67 0.11795812426834829 1102 68-69 0.11929025997357456 1102 70-71 0.16587223529476347 1102 72-73 0.17582446703407584 1102 74-75 0.21425406486380183 1102 76-77 0.20185826213555913 1102 78-79 0.1525550123375794 1102 80-81 0.1522873280623358 1102 82-83 0.135970481394164 1102 84-85 0.14358110817784464 1102 86-87 0.13199543946272385 1102 88-89 0.16589816725680961 1102 90-91 0.175903155228756 1102 92-93 0.2263699911718433 1102 94-95 0.16026303410195908 1102 96-97 0.21884561437179428 1102 98-99 0.23729005766910305 1102 100 0.1569915980552885 1103 1 -0.26378148074787333 1103 2 -0.12345389480291402 1103 3 -0.1351766233101479 1103 4 -0.11575207141061838 1103 5 -0.1306393240894934 1103 6 -0.18240668300082774 1103 7 -0.1933804428603807 1103 8 -0.24269312623815154 1103 9 -0.21335356050396115 1103 10-11 -0.206016986259975 1103 12-13 -0.2032531835115492 1103 14-15 -0.2709070583834645 1103 16-17 -0.26865989133631984 1103 18-19 -0.28630727969526504 1103 20-21 -0.36622905774226666 1103 22-23 -0.31761205732830433 1103 24-25 -0.25079445242248966 1103 26-27 -0.20525657042822587 1103 28-29 -0.298302432204018 1103 30-31 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-0.575279535173884 1103 96-97 -0.5914926980810264 1103 98-99 -0.7012455772261355 1103 100 -0.6757269259003174 1104 1 -0.04604428821411588 1104 2 0.016738310463047412 1104 3 -0.01936736709564002 1104 4 0.05796460893598265 1104 5 0.08091882507929427 1104 6 0.06506649102770723 1104 7 0.0683100886502217 1104 8 0.11988878828411487 1104 9 0.14666825878191503 1104 10-11 0.06421143867748214 1104 12-13 0.011895147321652644 1104 14-15 0.08872482693492145 1104 16-17 0.07246417926416626 1104 18-19 0.08991980212105233 1104 20-21 0.012389860367655103 1104 22-23 0.06674224533365702 1104 24-25 0.044305728461445426 1104 26-27 -0.013398903530848827 1104 28-29 0.023939430806201756 1104 30-31 -0.0393651600153575 1104 32-33 -0.11914479372191522 1104 34-35 -0.01978108031833159 1104 36-37 -0.11574004409553851 1104 38-39 -0.06698836902571514 1104 40-41 -0.07122004636055834 1104 42-43 -0.20960830451068801 1104 44-45 -0.19063393474744572 1104 46-47 -0.2562988120056957 1104 48-49 -0.23428757767835862 1104 50-51 -0.3568505025724562 1104 52-53 -0.3994719879101112 1104 54-55 -0.2850550755472625 1104 56-57 -0.39159778333704054 1104 58-59 -0.4238577316381509 1104 60-61 -0.4008592575483405 1104 62-63 -0.32421954643533724 1104 64-65 -0.3136594240344408 1104 66-67 -0.29079323491976794 1104 68-69 -0.2525705764233237 1104 70-71 -0.2856630000389515 1104 72-73 -0.3514387753415278 1104 74-75 -0.27937656390093224 1104 76-77 -0.2511790702312311 1104 78-79 -0.31241987504397883 1104 80-81 -0.28085649561428383 1104 82-83 -0.3509169346813614 1104 84-85 -0.3157531053916003 1104 86-87 -0.2629541627050713 1104 88-89 -0.3116654772658727 1104 90-91 -0.3426762127271985 1104 92-93 -0.3382273439564827 1104 94-95 -0.3589073002151686 1104 96-97 -0.3391438227948811 1104 98-99 -0.3636953752862446 1104 100 -0.35113695093589214 1105 1 -0.24882074383276986 1105 2 -0.21990308744251763 1105 3 -0.15538959058952528 1105 4 -0.13021540069127724 1105 5 -0.13365456260656572 1105 6 -0.1577402265130985 1105 7 -0.11882038096499059 1105 8 -0.18391317285001918 1105 9 -0.2539777103906431 1105 10-11 -0.3188137773468611 1105 12-13 -0.28037991638322524 1105 14-15 -0.24532582728138408 1105 16-17 -0.3057690159437456 1105 18-19 -0.2594320070302274 1105 20-21 -0.22677512601615746 1105 22-23 -0.2324411876945902 1105 24-25 -0.24529632221297248 1105 26-27 -0.21027403186631233 1105 28-29 -0.19666417504151212 1105 30-31 -0.2151189309740147 1105 32-33 -0.20772238239540286 1105 34-35 -0.2202157546239789 1105 36-37 -0.2491523703587788 1105 38-39 -0.3119716054692887 1105 40-41 -0.3131340035360708 1105 42-43 -0.3020577866245162 1105 44-45 -0.34163436225179566 1105 46-47 -0.32132892244993627 1105 48-49 -0.3782206681982032 1105 50-51 -0.2467391831324548 1105 52-53 -0.27047906361204355 1105 54-55 -0.3239628355927735 1105 56-57 -0.3830879052935998 1105 58-59 -0.34944211662097047 1105 60-61 -0.4945827026274898 1105 62-63 -0.3941235262652114 1105 64-65 -0.37660080544574726 1105 66-67 -0.3924074341233279 1105 68-69 -0.37489382693750883 1105 70-71 -0.3775583422612314 1105 72-73 -0.29348615090241026 1105 74-75 -0.24531749424465232 1105 76-77 -0.37490653789069484 1105 78-79 -0.36864331170769304 1105 80-81 -0.4145841820751883 1105 82-83 -0.4361227931740572 1105 84-85 -0.4374124955078358 1105 86-87 -0.4620992549887859 1105 88-89 -0.4280120654781996 1105 90-91 -0.4300809415312301 1105 92-93 -0.48478867736611164 1105 94-95 -0.5338017032743494 1105 96-97 -0.4479090084563211 1105 98-99 -0.5307073050139586 1105 100 -0.4634166936253976 1106 1 -0.04554836765431958 1106 2 -0.028782518117505873 1106 3 0.007294709250246001 1106 4 0.048954583509477345 1106 5 -0.006794075243000464 1106 6 0.005333531556644289 1106 7 0.01658336759101786 1106 8 -0.05465998456552512 1106 9 -0.057023354619055056 1106 10-11 -0.020515236755038302 1106 12-13 0.027598109322923392 1106 14-15 -0.03445153678440249 1106 16-17 -0.0031615492072987195 1106 18-19 -0.04015755584515546 1106 20-21 -0.0651441418804879 1106 22-23 0.03827044884525321 1106 24-25 0.049514221660992064 1106 26-27 -0.06459096282893739 1106 28-29 -0.0844289175493671 1106 30-31 -0.06292843800214598 1106 32-33 -0.07114568966404278 1106 34-35 -0.015098621289020286 1106 36-37 -0.08493249329437447 1106 38-39 -0.08410302058304353 1106 40-41 -0.13096204821051316 1106 42-43 -0.12052917539962493 1106 44-45 -0.1324640624606772 1106 46-47 -0.1271187234899287 1106 48-49 -0.1772751298898072 1106 50-51 -0.2225180831553999 1106 52-53 -0.2920930749036046 1106 54-55 -0.2582223554088898 1106 56-57 -0.25798908606179083 1106 58-59 -0.27312799507002694 1106 60-61 -0.3050926670644074 1106 62-63 -0.23990492142444708 1106 64-65 -0.24493211812412596 1106 66-67 -0.16051885264283072 1106 68-69 -0.19950400709358007 1106 70-71 -0.22972136900948925 1106 72-73 -0.2781791636660742 1106 74-75 -0.2428506624628639 1106 76-77 -0.21478581800278107 1106 78-79 -0.26065001603042504 1106 80-81 -0.26927909824321006 1106 82-83 -0.24540996002076199 1106 84-85 -0.2504114652368017 1106 86-87 -0.23153363816414085 1106 88-89 -0.22807291524469875 1106 90-91 -0.20375059666328355 1106 92-93 -0.22676119273306483 1106 94-95 -0.30433123431364706 1106 96-97 -0.24554773507551175 1106 98-99 -0.2535436043487813 1106 100 -0.2676887351465709 1107 1 -0.3084601379905969 1107 2 -0.34680076810780847 1107 3 -0.3102371971288491 1107 4 -0.2513864374112842 1107 5 -0.16642755551300326 1107 6 -0.3045100285029605 1107 7 -0.35505132348763624 1107 8 -0.26416191445213144 1107 9 -0.3358340108895277 1107 10-11 -0.3674835174805082 1107 12-13 -0.3278530627981979 1107 14-15 -0.37226520949474207 1107 16-17 -0.40345121657802707 1107 18-19 -0.44623646619433543 1107 20-21 -0.36345152694746474 1107 22-23 -0.4097942935035803 1107 24-25 -0.38327796208178455 1107 26-27 -0.32083469194000713 1107 28-29 -0.41827013743908736 1107 30-31 -0.38836075857656027 1107 32-33 -0.3741816256053383 1107 34-35 -0.39136847128791885 1107 36-37 -0.42061495359597956 1107 38-39 -0.505998792276344 1107 40-41 -0.45472464890948316 1107 42-43 -0.5014361082731682 1107 44-45 -0.4860543698348465 1107 46-47 -0.5367699843053657 1107 48-49 -0.5322211998441446 1107 50-51 -0.5451079695042083 1107 52-53 -0.5218445128190368 1107 54-55 -0.48472617429808196 1107 56-57 -0.4529223520994705 1107 58-59 -0.4838548586363274 1107 60-61 -0.5974681960316204 1107 62-63 -0.5729363488992263 1107 64-65 -0.5065440327890514 1107 66-67 -0.4945504317893423 1107 68-69 -0.46502320062319313 1107 70-71 -0.46124076070640996 1107 72-73 -0.4561092420319426 1107 74-75 -0.4097232777614437 1107 76-77 -0.43689117246412934 1107 78-79 -0.398045212621696 1107 80-81 -0.43038233539271786 1107 82-83 -0.519460897355323 1107 84-85 -0.5287503358178398 1107 86-87 -0.5408483672857862 1107 88-89 -0.5407008381723664 1107 90-91 -0.5881034280989041 1107 92-93 -0.4883866231777816 1107 94-95 -0.5089310886702982 1107 96-97 -0.48430281471600267 1107 98-99 -0.5624748091899683 1107 100 -0.5724341687209211 1108 1 -0.3313466048317544 1108 2 -0.3489496745274039 1108 3 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0.12893556302588394 2214 2 0.12396717574560157 2214 3 0.04013210734679973 2214 4 -0.011512668933605141 2214 5 0.006893082782937654 2214 6 0.02689709251186656 2214 7 -0.014604163840807871 2214 8 0.025833431463460954 2214 9 0.07150393254098475 2214 10-11 0.03478751850494888 2214 12-13 0.06536359811543946 2214 14-15 0.016117746371385522 2214 16-17 0.0938002554256272 2214 18-19 0.054233398166338986 2214 20-21 0.0733389715023165 2214 22-23 0.1029841793854871 2214 24-25 0.18226043599653252 2214 26-27 0.15849061195377345 2214 28-29 0.1541624276012996 2214 30-31 0.15249571473008672 2214 32-33 0.1659140200359559 2214 34-35 0.12747376211464 2214 36-37 0.1207266338364903 2214 38-39 0.16187131426498524 2214 40-41 0.20727847720933568 2214 42-43 0.12498585621541736 2214 44-45 0.2397027657821269 2214 46-47 0.28452078570647643 2214 48-49 0.3470851013477372 2214 50-51 0.3077452477146636 2214 52-53 0.23132801953648396 2214 54-55 0.25058683501915624 2214 56-57 0.30540282957476705 2214 58-59 0.27715284252273875 2214 60-61 0.3355922264715687 2214 62-63 0.2705356611670666 2214 64-65 0.24534433649461107 2214 66-67 0.2550009870975103 2214 68-69 0.2555017156663837 2214 70-71 0.2935991715591726 2214 72-73 0.22900915149857326 2214 74-75 0.23848725224843292 2214 76-77 0.29046029703755494 2214 78-79 0.2893202933062824 2214 80-81 0.30953873226575723 2214 82-83 0.31646037555225703 2214 84-85 0.32842030855586657 2214 86-87 0.3426261845925289 2214 88-89 0.3121245087860842 2214 90-91 0.2594905021513796 2214 92-93 0.34592025036463525 2214 94-95 0.37422772941367555 2214 96-97 0.3769560675110881 2214 98-99 0.3885455191067564 2214 100 0.41134445406420994 2215 1 0.04918841752915526 2215 2 -0.03739956185852833 2215 3 -0.016765570012886144 2215 4 -0.07130671567541214 2215 5 0.0076624950760972865 2215 6 0.01636064808625548 2215 7 0.038522203108691144 2215 8 -0.05747970781150258 2215 9 -0.05797049048614156 2215 10-11 -0.05373018035813715 2215 12-13 -0.06531210690145883 2215 14-15 -0.045354725330952306 2215 16-17 0.05821996593430612 2215 18-19 0.0745931862762177 2215 20-21 0.019954150165595763 2215 22-23 0.09862777024876834 2215 24-25 0.16690215450654478 2215 26-27 0.10260262195458836 2215 28-29 0.09246895697824442 2215 30-31 0.0502063059318445 2215 32-33 0.04819118544732248 2215 34-35 0.06879441805997288 2215 36-37 0.0404180997402932 2215 38-39 0.06290321353391448 2215 40-41 0.1162787527695528 2215 42-43 0.1317710831633363 2215 44-45 0.14230478420470405 2215 46-47 0.1789174899241388 2215 48-49 0.25572006359169563 2215 50-51 0.3141202847967435 2215 52-53 0.3070077814121319 2215 54-55 0.3137760039344002 2215 56-57 0.3268624315321489 2215 58-59 0.25536904658367376 2215 60-61 0.360119489477178 2215 62-63 0.3131656995457277 2215 64-65 0.3154940146062941 2215 66-67 0.33093052209338225 2215 68-69 0.31937196731671236 2215 70-71 0.34301488028381044 2215 72-73 0.34720560521033406 2215 74-75 0.3773306993200549 2215 76-77 0.3614100052443021 2215 78-79 0.426991442618295 2215 80-81 0.4160243434857662 2215 82-83 0.4083079470658326 2215 84-85 0.3971486392266321 2215 86-87 0.3452870162653774 2215 88-89 0.3446540330283838 2215 90-91 0.3995602015237729 2215 92-93 0.4180436331986144 2215 94-95 0.313109206035584 2215 96-97 0.30622448780613354 2215 98-99 0.32150366457155855 2215 100 0.36462444210086176 2216 1 0.20585457640506277 2216 2 0.141996288228615 2216 3 0.17615693489095108 2216 4 0.19992624941536974 2216 5 0.1566625271200337 2216 6 0.18854061438700143 2216 7 0.19555168370386156 2216 8 0.21050876562381404 2216 9 0.22267371092321042 2216 10-11 0.16945557578542747 2216 12-13 0.16192305208531366 2216 14-15 0.2290240506488459 2216 16-17 0.24103710625437458 2216 18-19 0.2511499776463495 2216 20-21 0.20929945051069154 2216 22-23 0.2648457860709357 2216 24-25 0.273187487447359 2216 26-27 0.2552694359319858 2216 28-29 0.230770511691901 2216 30-31 0.19661075750325807 2216 32-33 0.23719464807766144 2216 34-35 0.22310534742501886 2216 36-37 0.23394318491805421 2216 38-39 0.3140901244587866 2216 40-41 0.2776959396647172 2216 42-43 0.40183904972865037 2216 44-45 0.4566795581192906 2216 46-47 0.521896684325803 2216 48-49 0.487567793364299 2216 50-51 0.5531266005371549 2216 52-53 0.5065187429239302 2216 54-55 0.49552984667953837 2216 56-57 0.4907001995809779 2216 58-59 0.5743633955408001 2216 60-61 0.5568019736261363 2216 62-63 0.4896320995908283 2216 64-65 0.4763573083085362 2216 66-67 0.4539349729450919 2216 68-69 0.39068819524131015 2216 70-71 0.3704324755249928 2216 72-73 0.39793535879587694 2216 74-75 0.3577954203998459 2216 76-77 0.41226994804158323 2216 78-79 0.4319907469049653 2216 80-81 0.4197039130071474 2216 82-83 0.42263368689954817 2216 84-85 0.4217061415609571 2216 86-87 0.3832003295281261 2216 88-89 0.4070036771264647 2216 90-91 0.4349120559682049 2216 92-93 0.41164718318724525 2216 94-95 0.42501516581410215 2216 96-97 0.38258594395126266 2216 98-99 0.36876472602789434 2216 100 0.420830367762008 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 91909.0 3 17105.0 4 12049.0 5 10353.0 6 7519.0 7 6791.0 8 7647.0 9 8041.0 10 8381.0 11 8940.0 12 9877.0 13 10792.0 14 11663.0 15 11933.0 16 12178.0 17 12524.0 18 12568.0 19 11536.0 20 11230.0 21 11581.0 22 13597.0 23 20169.0 24 32258.0 25 42257.0 26 47845.0 27 37903.0 28 34141.0 29 42355.0 30 51114.0 31 57068.0 32 72592.0 33 96966.0 34 136159.0 35 216294.0 36 364379.0 37 656166.0 38 1012383.0 39 522055.0 40 1699.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.231595046975976 21.67356544781376 14.138157905276913 21.956681599933354 2 14.785490126346243 22.443906187340172 40.46075448908027 22.309849197233316 3 17.311649532932393 29.2106714974736 30.59513134557449 22.882547624019523 4 11.792975378178522 19.296069293012092 39.290782603587736 29.62017272522165 5 11.910640089919639 40.5557275033334 34.794509646626395 12.739122760120566 6 27.14373698503274 40.74257231633943 19.009434125827642 13.104256572800196 7 24.693584465592636 35.73630685992028 22.98392582116696 16.586182853320118 8 24.28991187724851 37.18203491785016 21.036456484440695 17.491596720460638 9 25.09510134720686 18.72829817125923 21.57888504737999 34.59771543415392 10-11 22.25340545782788 29.499764680967004 29.094501162992138 19.152328698212976 12-13 23.002211415126975 27.742470826310694 28.695798524638565 20.559519233923766 14-15 21.006253393945148 29.156709316132723 26.354097431963737 23.482939857958396 16-17 20.152915455102505 32.09831022221215 27.051460111229424 20.697314211455925 18-19 20.197999335868058 30.920982968616435 28.916904825091915 19.96411287042359 20-21 22.131332278714815 29.77615628624918 28.15272657828035 19.93978485675565 22-23 21.638212857730778 29.869804787195882 28.201983680656596 20.28999867441675 24-25 21.178774070592446 29.911913137095475 28.23063067195955 20.67868212035253 26-27 20.81967971754736 29.97873256659259 28.260814190487228 20.94077352537282 28-29 20.7599902433703 29.99863572550797 28.47544926039119 20.765924770730535 30-31 21.07546126777704 30.28711997796771 28.09501359347087 20.542405160784387 32-33 20.765585041177093 30.03661875208713 28.53069957846693 20.667096628268855 34-35 21.043499111677868 29.843342705102927 28.34531765817962 20.76784052503959 36-37 21.128696039316473 29.90577282965205 28.120902394512104 20.844628736519375 38-39 20.982130770362375 29.9776429571047 28.300297911631063 20.739928360901864 40-41 20.877485190248578 29.73482279710157 28.50239168533431 20.885300327315544 42-43 20.8309115783583 29.767180767793466 28.485093236788554 20.916814417059683 44-45 20.995205281113353 29.85629181640576 28.390479281150967 20.758023621329915 46-47 21.07100988667599 30.021765891301243 28.173012161958503 20.73421206006426 48-49 20.963969290649537 29.77500163053895 28.239507431856474 21.02152164695504 50-51 20.898291571115983 29.766506450054592 28.244746501824675 21.09045547700475 52-53 20.92999404234695 29.832304136654454 28.20379894741461 21.03390287358399 54-55 21.01092334982728 30.026128012590526 28.10931352281366 20.85363511476853 56-57 21.0134932925629 29.819835150736623 27.96512090030679 21.20155065639368 58-59 21.0253408279743 29.596522845103994 28.214978505190448 21.163157821731257 60-61 21.1366741478805 29.89859016374202 27.92475538458009 21.039980303797385 62-63 20.984015423800443 29.81261288029231 28.123270913635068 21.08010078227218 64-65 21.063735071386354 29.71436923740043 28.14782501541672 21.074070675796495 66-67 20.86096292200554 29.71490078687148 28.072248890482136 21.351887400640837 68-69 21.220610669411062 29.916007717351917 27.891796506120553 20.971585107116468 70-71 21.132423654771998 29.667441283005765 28.005314474408188 21.194820587814046 72-73 20.99963061593792 29.749898986127427 28.21828864497391 21.032181752960742 74-75 21.066646929504323 29.88839834927976 28.031998207652286 21.012956513563633 76-77 21.05274178206899 29.78766932920216 27.942939266343707 21.21664962238514 78-79 21.151812645005798 29.743170782807603 28.135575996341622 20.969440575844974 80-81 21.006712895288864 29.845856282262762 28.106761336113166 21.040669486335204 82-83 20.949911119552205 29.742980258510865 28.316905210549564 20.990203411387366 84-85 20.909671216930033 29.673209570864657 28.083031502931345 21.33408770927397 86-87 21.172600944880546 29.736529490539926 27.967869313441838 21.12300025113769 88-89 21.017626644828972 29.622499579873402 28.112324513786824 21.2475492615108 90-91 20.979689420963243 29.598795177187746 28.02780151045644 21.39371389139256 92-93 21.065340719660096 29.637756149863844 28.034163983922205 21.262739146553862 94-95 20.925308202467114 29.556404222090077 28.174539088233065 21.343748487209744 96-97 20.95248687659054 29.709614685256213 28.023798502738433 21.31409993541481 98-99 20.86323288010324 29.684161558114262 28.007488512759064 21.44511704902343 100 20.922692455233076 29.821631216457817 27.87833874596587 21.37733758234324 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 18895.0 1 13472.0 2 7284.5 3 5572.0 4 3851.0 5 2707.0 6 2246.0 7 2361.5 8 2876.5 9 3021.5 10 2324.5 11 1593.0 12 1415.5 13 1460.5 14 1519.5 15 1650.0 16 1896.5 17 2353.0 18 2960.5 19 3812.5 20 5126.5 21 6920.5 22 10029.0 23 15148.5 24 21017.5 25 27527.0 26 36467.0 27 48260.5 28 63184.5 29 77138.5 30 86177.5 31 94976.0 32 104139.5 33 112686.0 34 120649.5 35 126161.5 36 128242.5 37 128991.0 38 129763.5 39 129217.0 40 128555.5 41 126297.0 42 121135.0 43 115652.5 44 111894.0 45 109705.0 46 108701.0 47 105758.5 48 102625.5 49 102208.0 50 101197.5 51 97084.0 52 91856.5 53 87728.5 54 85442.5 55 83859.0 56 76834.5 57 67626.0 58 61684.5 59 56853.5 60 50712.0 61 42914.5 62 35829.0 63 29032.0 64 22886.0 65 18414.0 66 14486.5 67 11068.0 68 8517.0 69 6652.5 70 5052.0 71 3891.5 72 3071.0 73 2366.0 74 1960.5 75 1537.5 76 1077.0 77 835.5 78 664.5 79 499.5 80 372.0 81 283.5 82 213.0 83 177.0 84 151.0 85 126.5 86 124.0 87 110.0 88 91.5 89 86.0 90 69.5 91 49.5 92 44.0 93 38.0 94 34.0 95 31.5 96 31.5 97 31.0 98 31.0 99 35.5 100 46.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18419426137994577 2 0.17577212469986142 3 0.1909106488590004 4 0.18022306402129842 5 0.19562811149309825 6 0.07910411919775416 7 0.0805699974173891 8 0.0689762333166401 9 0.06217988884378722 10-11 0.07599912260525472 12-13 0.07553270680809815 14-15 0.07177472809957951 16-17 0.06821664187555654 18-19 0.07361373895693969 20-21 0.07446661355745457 22-23 0.07260095036882828 24-25 0.07213453457167171 26-27 0.07615903659285125 28-29 0.07386693610396754 30-31 0.07927735935098375 32-33 0.07582588245202514 34-35 0.07499966018277636 36-37 0.08681996909928713 38-39 0.0822624204527858 40-41 0.0768253448745035 42-43 0.0803301264359943 44-45 0.09097773277679713 46-47 0.0808098683987839 48-49 0.08679331676802104 50-51 0.08352840618792506 52-53 0.08148950284606919 54-55 0.08440793311970601 56-57 0.07715849901532962 58-59 0.08542072170781742 60-61 0.0818493093181614 62-63 0.0732006278223153 64-65 0.07571927312696078 66-67 0.07638558140861303 68-69 0.07471981070448241 70-71 0.07020224055488022 72-73 0.06748370276573906 74-75 0.07314732315978313 76-77 0.07886424821635936 78-79 0.07498633401714332 80-81 0.08296870723133717 82-83 0.08424801913210947 84-85 0.08590046367060704 86-87 0.08202254947139098 88-89 0.08387488649438422 90-91 0.08219578962462057 92-93 0.07321395398794835 94-95 0.07293410450965442 96-97 0.07210788224040562 98-99 0.08195591864322577 100 0.09005822734811703 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3752017.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.27309777410752 #Duplication Level Percentage of deduplicated Percentage of total 1 74.62801035600401 44.98061364874538 2 14.967087892905859 18.042255039255515 3 5.001622409887657 9.043898296209782 4 2.099941643822124 5.0627995207204375 5 1.0635381515620903 3.205136949779773 6 0.6111254482258114 2.210065433589577 7 0.3761843789630438 1.587165849803202 8 0.2411492596511172 1.1627850324084341 9 0.17798798352733203 0.965509842038321 >10 0.7678328601232248 7.839318626973335 >50 0.03991523459824991 1.6444634619858935 >100 0.023393760440684336 2.675697602593751 >500 0.0017684962631636355 0.6966368935771773 >1k 3.9791162313536013E-4 0.3507434060955432 >5k 0.0 0.0 >10k+ 4.4212402570594125E-5 0.5329103962238652 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 19998 0.5329933206592614 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.6652331266089678E-5 0.0 5 0.0 0.0 0.0 2.6652331266089678E-5 0.0 6 0.0 0.0 0.0 2.6652331266089678E-5 0.0 7 0.0 0.0 0.0 2.6652331266089678E-5 0.0 8 0.0 0.0 0.0 2.6652331266089678E-5 0.0 9 0.0 0.0 0.0 2.6652331266089678E-5 0.0 10-11 0.0 0.0 0.0 3.997849689913452E-5 0.0 12-13 0.0 0.0 0.0 5.3304662532179356E-5 0.0 14-15 0.0 0.0 0.0 6.66308281652242E-5 0.0 16-17 0.0 0.0 0.0 1.0660932506435871E-4 0.0 18-19 2.6652331266089678E-5 0.0 0.0 1.332616563304484E-4 0.0 20-21 2.6652331266089678E-5 0.0 0.0 2.2654481576176228E-4 0.0 22-23 2.6652331266089678E-5 0.0 0.0 4.664157971565694E-4 0.0 24-25 2.6652331266089678E-5 0.0 0.0 9.99462422478363E-4 0.0 26-27 2.6652331266089678E-5 0.0 0.0 0.001585813710332336 0.0 28-29 2.6652331266089678E-5 0.0 0.0 0.0034514768989586135 0.0 30-31 2.6652331266089678E-5 0.0 0.0 0.007702523735899917 0.0 32-33 2.6652331266089678E-5 0.0 0.0 0.0149786101715424 0.0 34-35 2.6652331266089678E-5 0.0 0.0 0.02651906960975923 0.0 36-37 2.6652331266089678E-5 0.0 0.0 0.04593529293710556 0.0 38-39 2.6652331266089678E-5 0.0 0.0 0.07277419052205786 0.0 40-41 2.6652331266089678E-5 0.0 0.0 0.10053259353569027 0.0 42-43 2.6652331266089678E-5 0.0 0.0 0.12825101805242353 0.0 44-45 2.6652331266089678E-5 0.0 0.0 0.16420501293037854 0.0 46-47 2.6652331266089678E-5 0.0 0.0 0.20876771080728046 0.0 48-49 2.6652331266089678E-5 0.0 0.0 0.2571949967177654 0.0 50-51 2.6652331266089678E-5 0.0 0.0 0.30571556578768166 0.0 52-53 2.6652331266089678E-5 0.0 0.0 0.35283688746612824 0.0 54-55 2.6652331266089678E-5 0.0 0.0 0.40862021680605387 0.0 56-57 2.6652331266089678E-5 0.0 0.0 0.47986189828031167 0.0 58-59 2.6652331266089678E-5 0.0 0.0 0.559618999594085 0.0 60-61 2.6652331266089678E-5 0.0 0.0 0.6462257500432435 0.0 62-63 2.6652331266089678E-5 0.0 0.0 0.7324060631921444 0.0 64-65 2.6652331266089678E-5 0.0 0.0 0.8259290936048531 0.0 66-67 2.6652331266089678E-5 0.0 0.0 0.9365762468560244 0.0 68-69 2.6652331266089678E-5 0.0 0.0 1.0650138312273105 0.0 70-71 2.6652331266089678E-5 0.0 0.0 1.208403373438873 0.0 72-73 2.6652331266089678E-5 0.0 0.0 1.360588184968245 0.0 74-75 2.6652331266089678E-5 0.0 0.0 1.5131194768040763 0.0 76-77 2.6652331266089678E-5 0.0 0.0 1.6820286262029196 0.0 78-79 2.6652331266089678E-5 0.0 0.0 1.880695103460352 0.0 80-81 2.6652331266089678E-5 0.0 0.0 2.103228743366568 0.0 82-83 2.6652331266089678E-5 0.0 0.0 2.3396615740280495 0.0 84-85 2.6652331266089678E-5 0.0 0.0 2.582397681033961 0.0 86-87 2.6652331266089678E-5 0.0 0.0 2.8403522691928105 0.0 88 2.6652331266089678E-5 0.0 0.0 3.04684120567684 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5275 0.0 49.243553 1 GTATCAA 9150 0.0 38.057774 1 TCAACGC 10685 0.0 31.265427 4 ATCAACG 10775 0.0 31.049604 3 TATCAAC 11755 0.0 29.34169 2 CAACGCA 11655 0.0 28.74292 5 GTACATG 20960 0.0 27.345682 1 TACATGG 20865 0.0 26.588827 2 AACGCAG 12665 0.0 26.346895 6 CATGGGT 10430 0.0 25.308075 4 ACATGGG 21385 0.0 25.018139 3 TACCTGG 2845 0.0 24.809198 2 CGCAGAG 13905 0.0 23.25286 8 ACGCAGA 14295 0.0 23.144787 7 TATACCG 270 4.5474735E-11 22.653887 5 ATGGGTG 5530 0.0 21.78101 5 GTGGTAT 2590 0.0 21.621208 1 GTACCTG 3445 0.0 21.036055 1 TGGTATC 2655 0.0 20.381544 2 GCAGAGT 15605 0.0 20.298355 9 >>END_MODULE