FastQCFastQC Report
Wed 25 May 2016
SRR2431393_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431393_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3752017
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278950.7434667806675715No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233600.6225984583758549No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142440.3796358065541814No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60210.16047368655312597No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51430.13707293970149922No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46120.12292055179920561No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44890.11964231505347657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111250.063.409041
GTATCAA214800.044.860861
GTGGTAT38200.035.0866131
ATCAACG277900.034.601523
TATCAAC280300.034.407742
TGGTATC37600.034.392082
TCAACGC280050.034.369464
CAACGCA289700.033.257065
AACGCAG301350.032.0179866
CGCAGAG334550.028.5455828
ACGCAGA336100.028.4978377
GCAGAGT358950.026.3956769
CATGGGT99200.025.7856244
GTACATG202150.025.7300911
TATAACG3900.025.3202672
TACATGG204250.024.7261092
ACATGGG204600.024.1768973
GAGTACT245700.023.32667712-13
CAGAGTA353650.022.88420110-11
ATGGGTA45500.022.4253255
Produced by FastQC (version 0.11.5)