##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431393_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3752017 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.624696263369806 34.0 31.0 34.0 31.0 34.0 2 32.74470291579169 34.0 31.0 34.0 31.0 34.0 3 32.85527171118894 34.0 31.0 34.0 31.0 34.0 4 36.324970009464245 37.0 37.0 37.0 35.0 37.0 5 36.22319088639524 37.0 37.0 37.0 35.0 37.0 6 36.2666453803381 37.0 37.0 37.0 35.0 37.0 7 36.22702855557424 37.0 37.0 37.0 35.0 37.0 8 36.240090863127755 37.0 37.0 37.0 35.0 37.0 9 38.035738377518015 39.0 39.0 39.0 35.0 39.0 10-11 38.01576498720555 39.0 39.0 39.0 35.0 39.0 12-13 37.99752599735022 39.0 39.0 39.0 35.0 39.0 14-15 39.46076030572356 41.0 39.0 41.0 36.0 41.0 16-17 39.48696541087101 41.0 39.0 41.0 36.0 41.0 18-19 39.56075878654068 41.0 39.5 41.0 36.5 41.0 20-21 39.56083901005779 41.0 40.0 41.0 37.0 41.0 22-23 39.49777879471228 41.0 39.5 41.0 36.0 41.0 24-25 39.44567108837727 41.0 39.0 41.0 36.0 41.0 26-27 39.329142431923955 41.0 39.0 41.0 36.0 41.0 28-29 39.24925686637347 41.0 39.0 41.0 35.5 41.0 30-31 39.06701608761368 41.0 39.0 41.0 35.0 41.0 32-33 38.83954137201404 41.0 38.0 41.0 35.0 41.0 34-35 38.698547874383294 41.0 38.0 41.0 35.0 41.0 36-37 38.550573731409 41.0 38.0 41.0 35.0 41.0 38-39 38.4030585415791 41.0 38.0 41.0 35.0 41.0 40-41 38.25518661029521 40.0 38.0 41.0 34.5 41.0 42-43 38.068677860468114 40.0 38.0 41.0 34.0 41.0 44-45 37.8045190093755 40.0 38.0 41.0 33.0 41.0 46-47 37.41022015625195 40.0 38.0 41.0 33.0 41.0 48-49 37.05325748790584 40.0 37.0 41.0 31.5 41.0 50-51 36.87160386000383 40.0 37.0 41.0 31.0 41.0 52-53 36.80190481546326 40.0 37.0 41.0 31.0 41.0 54-55 36.65805632010729 40.0 36.5 41.0 31.0 41.0 56-57 36.50332940922176 40.0 36.0 41.0 31.0 41.0 58-59 36.323694162366536 40.0 35.5 41.0 30.5 41.0 60-61 36.16863143210705 39.5 35.0 41.0 30.5 41.0 62-63 36.009213977442 39.0 35.0 41.0 30.0 41.0 64-65 35.776640137824536 39.0 35.0 41.0 30.0 41.0 66-67 35.51379644601823 38.5 35.0 41.0 29.5 41.0 68-69 35.22209134446885 37.5 35.0 41.0 29.0 41.0 70-71 34.926786978843644 37.0 35.0 40.0 29.0 41.0 72-73 34.61198323461754 37.0 35.0 39.5 29.0 41.0 74-75 34.27590893111625 36.0 35.0 39.0 28.5 41.0 76-77 33.94505448669342 36.0 35.0 39.0 28.0 41.0 78-79 33.62612336244745 35.0 34.0 38.0 28.0 39.5 80-81 33.35936750819626 35.0 34.0 37.0 28.0 39.0 82-83 33.16015439162456 35.0 34.0 37.0 29.0 39.0 84-85 32.89704470955222 35.0 34.0 36.5 28.5 38.5 86-87 32.65574236470677 35.0 34.0 36.0 28.0 37.0 88-89 32.42206511857489 35.0 34.0 36.0 27.0 37.0 90-91 32.23923812178889 35.0 34.0 35.5 27.0 36.5 92-93 32.077639307071365 35.0 34.0 35.0 27.0 36.0 94-95 31.94188898930895 35.0 34.0 35.0 26.5 36.0 96-97 31.801203592627644 35.0 34.0 35.0 26.0 36.0 98-99 31.68633004594595 35.0 34.0 35.0 26.0 36.0 100 31.58548935146083 35.0 34.0 35.0 25.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.035437872262640724 1101 2 0.009539790329505138 1101 3 0.027454615327272336 1101 4 0.05837838687155994 1101 5 0.04327004389266165 1101 6 0.02828033408800934 1101 7 0.05282170273493847 1101 8 0.06643303516588617 1101 9 0.08098245406736027 1101 10-11 0.035040195775998484 1101 12-13 0.03301113818077539 1101 14-15 0.015543788366329636 1101 16-17 0.007668653608185139 1101 18-19 -0.03933789757839179 1101 20-21 0.03149733493693674 1101 22-23 0.034721141220437346 1101 24-25 0.07432990896991498 1101 26-27 0.10071636026814446 1101 28-29 0.054779943586751756 1101 30-31 0.040374413862636516 1101 32-33 0.08302979068145078 1101 34-35 0.14085415033341064 1101 36-37 0.1530154456431987 1101 38-39 0.09385001721739172 1101 40-41 -0.006226033833058864 1101 42-43 0.04250252612386163 1101 44-45 0.06406693624708737 1101 46-47 0.11749969702142948 1101 48-49 0.2029286626304696 1101 50-51 0.14699710641743025 1101 52-53 0.19692042496848927 1101 54-55 0.20384096567837418 1101 56-57 0.24706970170266374 1101 58-59 0.2466959757873326 1101 60-61 0.22974968646846605 1101 62-63 0.19483588022774256 1101 64-65 0.2756761872406912 1101 66-67 0.24742242653216806 1101 68-69 0.3030906077877873 1101 70-71 0.3073550451226126 1101 72-73 0.23114720794591648 1101 74-75 0.2549643107937172 1101 76-77 0.17884497806122113 1101 78-79 0.22553148393539146 1101 80-81 0.1848578272633148 1101 82-83 0.2511673065734712 1101 84-85 0.26051177171234485 1101 86-87 0.2248004992185031 1101 88-89 0.258399692321305 1101 90-91 0.27960858994669024 1101 92-93 0.2906757685086845 1101 94-95 0.19802164524269372 1101 96-97 0.27563310183647616 1101 98-99 0.2510203334782979 1101 100 0.23022297898538469 1102 1 -0.04081113714585172 1102 2 -0.03293850660932662 1102 3 -0.015344064265775614 1102 4 0.03854746421811228 1102 5 -0.015052442248965292 1102 6 -0.024132081347282508 1102 7 -0.01679609429587714 1102 8 -0.029451974188731356 1102 9 0.027713717930076598 1102 10-11 0.01345445783720578 1102 12-13 -0.004089815330758029 1102 14-15 -0.008879835255207524 1102 16-17 -0.05495166344221758 1102 18-19 -0.01546652618095834 1102 20-21 0.039624311295263226 1102 22-23 0.06032077397791369 1102 24-25 0.07309905643271009 1102 26-27 0.054554009916003565 1102 28-29 0.04783669956485426 1102 30-31 0.020550867299547804 1102 32-33 0.08319197789182908 1102 34-35 0.08596571153066179 1102 36-37 0.07774496007083087 1102 38-39 0.03389238200931288 1102 40-41 0.03167275232280531 1102 42-43 0.01597177043039011 1102 44-45 -0.06152991962931509 1102 46-47 -0.06520432563791445 1102 48-49 -0.12548290932720363 1102 50-51 -0.10864508204254264 1102 52-53 -0.04973855320869802 1102 54-55 0.012085612372324306 1102 56-57 -0.043480745548741595 1102 58-59 -0.02815389907945587 1102 60-61 -0.10453446830099722 1102 62-63 -0.09988362007251084 1102 64-65 -0.0586924888465461 1102 66-67 -0.004623874488672186 1102 68-69 -0.10980436015658057 1102 70-71 -0.09143970171260918 1102 72-73 -0.07073846786015991 1102 74-75 -0.0930316488443168 1102 76-77 -0.10425848158548945 1102 78-79 -0.06950180392651362 1102 80-81 -0.08653786787679962 1102 82-83 -0.12257077453227794 1102 84-85 -0.07557293010269461 1102 86-87 -0.03541877025350715 1102 88-89 -0.08549689662740434 1102 90-91 -0.06553230205693694 1102 92-93 -0.06131030890381339 1102 94-95 -0.07197415472467483 1102 96-97 -0.07304631765653369 1102 98-99 -0.06985356835201273 1102 100 -0.06485673485702037 1103 1 -0.12125162506192311 1103 2 -0.10706799717492999 1103 3 -0.03184521337377788 1103 4 0.03165982167748638 1103 5 0.009736295447005716 1103 6 -0.0032761370398119993 1103 7 -0.08041924542396828 1103 8 -0.01773044519545408 1103 9 -0.008982559963762071 1103 10-11 -5.836896208464282E-4 1103 12-13 0.01807578796663023 1103 14-15 0.009389152212293084 1103 16-17 -0.006133132585681267 1103 18-19 -0.025641778221441314 1103 20-21 -0.09856795047132749 1103 22-23 -0.054729665485318435 1103 24-25 -0.09986125072103391 1103 26-27 -0.11627652645289999 1103 28-29 -0.07954552993470543 1103 30-31 -0.07828240643492279 1103 32-33 -0.09678661879530637 1103 34-35 -0.0641415141524817 1103 36-37 -0.08667890708015591 1103 38-39 -0.13756064885453156 1103 40-41 -0.17452087708097963 1103 42-43 -0.14146726620095507 1103 44-45 -0.1810242389161374 1103 46-47 -0.111656415467003 1103 48-49 -0.10631889324177735 1103 50-51 -0.16125744682108234 1103 52-53 -0.08777408040774048 1103 54-55 -0.014675586464129253 1103 56-57 -0.028756792868158243 1103 58-59 -0.09912187880329526 1103 60-61 -0.10597168978085136 1103 62-63 -0.07328637040343722 1103 64-65 -0.030225821343393022 1103 66-67 -0.07343573445433549 1103 68-69 -0.06702732475067563 1103 70-71 -0.03005898414223651 1103 72-73 -0.04226202405143198 1103 74-75 -0.06178971298943736 1103 76-77 0.0016797091601645775 1103 78-79 -0.059689268507014503 1103 80-81 -0.015533240076962329 1103 82-83 -0.0415378006742273 1103 84-85 -0.020109282275541318 1103 86-87 0.02734500701820508 1103 88-89 0.0242170915406561 1103 90-91 0.018130654841584715 1103 92-93 -0.01471084257508437 1103 94-95 -0.013575419475397865 1103 96-97 -0.05084730762510503 1103 98-99 -0.019254495166695307 1103 100 -0.008170891993096063 1104 1 -0.11536817126794574 1104 2 -0.09690336620303697 1104 3 -0.0797214266641646 1104 4 -0.04623187267068829 1104 5 -0.051649898194014554 1104 6 -0.04744159652704383 1104 7 -0.06060009505529962 1104 8 -0.07131048054887401 1104 9 -0.056081135277608496 1104 10-11 -0.1175847385590032 1104 12-13 -0.09114111065860442 1104 14-15 -0.09574179352166112 1104 16-17 -0.047775754296388584 1104 18-19 -0.11644453753773121 1104 20-21 -0.09595785270480661 1104 22-23 -0.09950947852903624 1104 24-25 -0.011712210589500671 1104 26-27 -0.030820435621542686 1104 28-29 -0.061115129082509156 1104 30-31 -0.04826159063034652 1104 32-33 0.012754189873398047 1104 34-35 0.0013505990848088345 1104 36-37 0.007521794327551845 1104 38-39 -0.02977725329678549 1104 40-41 -0.14085154046789228 1104 42-43 -0.05122105305638769 1104 44-45 -0.01898923028362276 1104 46-47 -0.02803299026795969 1104 48-49 -0.005838714570643333 1104 50-51 -0.06913568422760363 1104 52-53 -0.04758810187194484 1104 54-55 0.04744115872279764 1104 56-57 0.016901898266304727 1104 58-59 0.01644360864418104 1104 60-61 -0.03992833081156988 1104 62-63 -0.047404374659230086 1104 64-65 0.018737308008773823 1104 66-67 -0.05916218201900847 1104 68-69 -0.031502525468376064 1104 70-71 0.1128662925227033 1104 72-73 0.06425851172181751 1104 74-75 0.07957747454406672 1104 76-77 -0.0037753009990098008 1104 78-79 -0.008739709309764976 1104 80-81 0.01107513890619316 1104 82-83 0.007171799554257063 1104 84-85 -0.020424066642846128 1104 86-87 0.01084868344780432 1104 88-89 0.020099679081539534 1104 90-91 0.003979166901800113 1104 92-93 -0.0022503492911241096 1104 94-95 0.01088574965752187 1104 96-97 0.02863165400331269 1104 98-99 0.04154902748153333 1104 100 0.07728379224907655 1105 1 -0.1619132292166654 1105 2 -0.16515453679073744 1105 3 -0.14079978114268954 1105 4 -0.04262212018560518 1105 5 -0.12043336219107914 1105 6 -0.07904928218006546 1105 7 -0.06121149360043887 1105 8 -0.03491797769603977 1105 9 -0.006424215934551114 1105 10-11 -0.0635762625278602 1105 12-13 -0.111851912380736 1105 14-15 -0.13107884872358255 1105 16-17 -0.14735727129725973 1105 18-19 -0.19550471803209035 1105 20-21 -0.10728094500871777 1105 22-23 -0.1305898527131646 1105 24-25 -0.1549619000597673 1105 26-27 -0.08405997607119531 1105 28-29 -0.05450111501862409 1105 30-31 -0.09139072050751906 1105 32-33 -0.2218299359138598 1105 34-35 -0.16353377276992376 1105 36-37 -0.17697327927388784 1105 38-39 -0.11314056803760764 1105 40-41 -0.13080183887939256 1105 42-43 -0.20070713896792114 1105 44-45 -0.15788616807332545 1105 46-47 -0.2304258028089805 1105 48-49 -0.17440336029745396 1105 50-51 -0.19618314394289627 1105 52-53 -0.19291497439149197 1105 54-55 -0.20941070933655936 1105 56-57 -0.17733367682803447 1105 58-59 -0.1528788137907071 1105 60-61 -0.1849250319590965 1105 62-63 -0.2541166642311552 1105 64-65 -0.2277226866726707 1105 66-67 -0.21344281337898252 1105 68-69 -0.14416156886849052 1105 70-71 -0.1443476582570682 1105 72-73 -0.12665998305892145 1105 74-75 -0.1828513729109318 1105 76-77 -0.2379520521679126 1105 78-79 -0.12015648229149178 1105 80-81 -0.12417831731499973 1105 82-83 -0.2002067235696927 1105 84-85 -0.18528948692674874 1105 86-87 -0.22225195174961243 1105 88-89 -0.14620210532835642 1105 90-91 -0.167365839207811 1105 92-93 -0.20440007343299982 1105 94-95 -0.1621633493854766 1105 96-97 -0.17062396666904434 1105 98-99 -0.21585894968232466 1105 100 -0.1652621812995143 1106 1 -0.10060138583364164 1106 2 -0.06746386925795633 1106 3 -0.03122407923356718 1106 4 0.014563773714229455 1106 5 -0.026279969496073363 1106 6 -0.06602891147973367 1106 7 -0.014745776904590002 1106 8 -0.008882726252700479 1106 9 -0.03297611509817244 1106 10-11 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28-29 -0.17418062203216778 1107 30-31 -0.20961799225301547 1107 32-33 -0.15682405345836514 1107 34-35 -0.16503102749390308 1107 36-37 -0.20626712048220242 1107 38-39 -0.1601220135160446 1107 40-41 -0.26161552911379005 1107 42-43 -0.2934768191710475 1107 44-45 -0.2483452181940322 1107 46-47 -0.24363465431061115 1107 48-49 -0.27798691549689636 1107 50-51 -0.2641979134471768 1107 52-53 -0.28835726192112787 1107 54-55 -0.2272640138296893 1107 56-57 -0.2779695636319488 1107 58-59 -0.30685218413194093 1107 60-61 -0.2977304844137407 1107 62-63 -0.2594871860638932 1107 64-65 -0.2751471053817127 1107 66-67 -0.25828063013834424 1107 68-69 -0.19156998355128252 1107 70-71 -0.30887060946196243 1107 72-73 -0.2628605569659115 1107 74-75 -0.31481787349136425 1107 76-77 -0.24086816791007237 1107 78-79 -0.2867992838756521 1107 80-81 -0.24592751574749627 1107 82-83 -0.24750245949857685 1107 84-85 -0.29266983471114827 1107 86-87 -0.32931898493883693 1107 88-89 -0.2987211260484912 1107 90-91 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0.07221976758654591 2214 62-63 0.08500486999856349 2214 64-65 0.042736213932542455 2214 66-67 0.06077138578650931 2214 68-69 0.040812663002000704 2214 70-71 0.008990928427309086 2214 72-73 -0.0347368570706621 2214 74-75 0.025530896109977164 2214 76-77 -0.04043112237245339 2214 78-79 0.022382563733856387 2214 80-81 0.011775691386056053 2214 82-83 0.008727249457308517 2214 84-85 0.04427975947349694 2214 86-87 0.05931037502713821 2214 88-89 0.028912162434110655 2214 90-91 0.08921085616123037 2214 92-93 0.07547854033605716 2214 94-95 0.08798987020066207 2214 96-97 0.08954623872691769 2214 98-99 0.09364779220645758 2214 100 0.08309439676915531 2215 1 0.09389814222069504 2215 2 0.06206027270265224 2215 3 0.04402052543116497 2215 4 0.02058945588593275 2215 5 0.016039212857727136 2215 6 0.07467248818861805 2215 7 -0.008155760738659978 2215 8 0.020205051855235467 2215 9 0.004338442431475187 2215 10-11 0.01562891384349996 2215 12-13 -0.006894613253450643 2215 14-15 0.04147616124811293 2215 16-17 -0.003443832475440445 2215 18-19 0.019489337790517425 2215 20-21 0.02406883636886903 2215 22-23 -0.02273857958621761 2215 24-25 0.033585722806861895 2215 26-27 0.030580034759537966 2215 28-29 0.05836729945707475 2215 30-31 0.05974229368193562 2215 32-33 0.023992518594745604 2215 34-35 -0.03373503989563176 2215 36-37 -0.05415769315364116 2215 38-39 -0.020747666908206952 2215 40-41 -0.03306683048316472 2215 42-43 -0.048555232651146696 2215 44-45 0.026674999574638036 2215 46-47 0.022467512920748334 2215 48-49 -0.0038080747388704594 2215 50-51 0.05846325430031385 2215 52-53 -0.005965384043840061 2215 54-55 -0.0622814299512342 2215 56-57 -0.004965450946656347 2215 58-59 0.0012373151169740026 2215 60-61 -0.01400017259298636 2215 62-63 -0.005841112003032833 2215 64-65 -0.030303532641674735 2215 66-67 -0.00958045849732514 2215 68-69 -0.003256972147681836 2215 70-71 0.030091237947146965 2215 72-73 0.024376923721938226 2215 74-75 0.0023301867104379426 2215 76-77 -0.003109375387879254 2215 78-79 0.0354168093718954 2215 80-81 0.008669384697611804 2215 82-83 -0.011320503112017377 2215 84-85 0.011302029817116477 2215 86-87 1.6996701505433975E-4 2215 88-89 -0.04337798473856935 2215 90-91 -0.04882983041683531 2215 92-93 -0.01464540295305028 2215 94-95 -0.07373188921771856 2215 96-97 -0.09460243049085904 2215 98-99 -0.08569868948139359 2215 100 -0.01875016710545907 2216 1 0.13443963680826698 2216 2 0.11517622875070543 2216 3 0.05289978095621706 2216 4 0.020953334518573286 2216 5 0.03542592442475012 2216 6 0.05753583518318095 2216 7 0.06849706820136703 2216 8 0.03305638494307317 2216 9 0.05853215438251169 2216 10-11 0.037917336882436814 2216 12-13 0.026479663208974102 2216 14-15 0.03263038478490898 2216 16-17 0.013164845228850197 2216 18-19 -0.010431711598791082 2216 20-21 0.01784890647826387 2216 22-23 -0.1047882632851227 2216 24-25 -0.05330287412613899 2216 26-27 -0.04152488960563261 2216 28-29 -0.01902759513806984 2216 30-31 -0.09718726380155118 2216 32-33 5.336778347810878E-4 2216 34-35 0.0578486366819746 2216 36-37 0.04202930087984669 2216 38-39 0.03636412120012977 2216 40-41 0.04793000847651285 2216 42-43 0.08493300542401272 2216 44-45 0.1404793699566511 2216 46-47 0.15055741827460167 2216 48-49 0.1290414907915931 2216 50-51 0.1206139089648417 2216 52-53 0.11310310293561088 2216 54-55 0.1290867511371232 2216 56-57 0.059271358388649276 2216 58-59 0.058066619624696614 2216 60-61 0.0757387284232891 2216 62-63 0.05256301501640337 2216 64-65 0.028591856235514967 2216 66-67 0.019721495994453164 2216 68-69 0.05787645881399328 2216 70-71 0.05746629357422961 2216 72-73 0.07969877081682597 2216 74-75 0.10344886289478694 2216 76-77 0.1363194148403295 2216 78-79 0.1475717094299327 2216 80-81 0.13074181959315467 2216 82-83 0.1568695001386402 2216 84-85 0.16315778513988022 2216 86-87 0.10086787372332395 2216 88-89 0.12578259000974157 2216 90-91 0.1315680139342703 2216 92-93 0.12437602655962365 2216 94-95 0.08492418541347391 2216 96-97 0.12990779418170462 2216 98-99 0.1196340088564547 2216 100 0.14179382245404426 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49.0 3 272.0 4 63.0 5 112.0 6 297.0 7 689.0 8 1359.0 9 2430.0 10 3437.0 11 4983.0 12 7580.0 13 11076.0 14 12595.0 15 16810.0 16 22460.0 17 34562.0 18 40082.0 19 19809.0 20 12065.0 21 10793.0 22 10528.0 23 10309.0 24 11016.0 25 12736.0 26 15897.0 27 19872.0 28 24750.0 29 31255.0 30 39531.0 31 50367.0 32 63301.0 33 82575.0 34 115197.0 35 188582.0 36 310743.0 37 644286.0 38 1157817.0 39 755007.0 40 6725.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.789630128749145 21.06326789971299 14.725789702627438 22.42131226891043 2 14.3977431861397 21.997165122801142 40.5249906845658 23.08010100649335 3 16.48124896221434 28.536513663855796 32.1002596229883 22.88197775094157 4 11.127588174573836 18.690320432983114 39.68883936293466 30.493252029508394 5 11.293313267661276 39.13035363080585 35.48818884200736 14.088144259525507 6 27.103102144793056 40.045287641287345 18.642799326335673 14.208810887583931 7 23.977210124580992 35.46940219087493 23.488539630817236 17.064848053726838 8 24.9639060803829 35.06362044734872 21.1298083137683 18.842665158500083 9 25.84495908333013 15.841616880098591 22.354382284084075 35.959041752487195 10-11 22.755294019190213 27.614866883598875 29.459288164206075 20.170550933004836 12-13 24.43686955576161 26.00368015390122 29.6831144421787 19.876335848158472 14-15 21.721330153887898 26.93551228579188 28.66940634863861 22.673751211681612 16-17 19.278057642062922 30.265081421539403 29.222135720600413 21.234725215797262 18-19 19.157407644317082 27.79348488919024 33.41263736615196 19.636470100340723 20-21 20.881102084558787 26.72008149216808 33.28076605196618 19.118050371306953 22-23 20.334870684835924 26.809923892905026 33.46394757697563 19.391257845283423 24-25 20.002995723231955 26.90579236679593 33.33519011315385 19.75602179681827 26-27 19.685808616042724 27.021791638487347 33.35204773438255 19.940352011087384 28-29 19.610206314721136 26.995606755364893 33.58427435104459 19.809912578869383 30-31 19.918614354481182 27.28849820083246 33.16045280226908 19.632434642417284 32-33 19.689011248362025 27.118244729400047 33.493164987925674 19.699579034312258 34-35 19.96248652380007 27.03241084385998 33.15105204311851 19.85405058922144 36-37 20.134369321736767 27.057661003645418 32.81993671328881 19.988032961329008 38-39 19.979250969320887 27.211414232342502 32.87466026294099 19.934674535395622 40-41 19.975882241157866 27.08939508908166 32.82796380710377 20.106758862656708 42-43 19.981164679523033 27.12709923404718 32.63598404421543 20.255752042214358 44-45 20.29742704748452 27.335756477357155 32.14392276145136 20.222893713706977 46-47 20.474819720131602 27.67948462872002 31.483442157124436 20.36225349402394 48-49 20.593507755166225 27.714468092478306 30.893653143710782 20.79837100864469 50-51 20.58860019720131 27.874001183634288 30.648745396959253 20.88865322220515 52-53 20.58762581468293 28.082822038879833 30.504519393874702 20.825032752562535 54-55 20.692877830931806 28.257052459375547 30.406898847517944 20.643170862174703 56-57 20.622677453079305 27.955625652578632 30.343455423423997 21.078241470918062 58-59 20.57099977132184 27.670350570549346 30.719912430656947 21.03873722747187 60-61 20.632819727455104 27.90938571187555 30.51701653185301 20.940778028816336 62-63 20.531131220827014 27.7538511071375 30.744384354578248 20.970633317457242 64-65 20.54960370025464 27.71355649503539 30.79624025642078 20.94059954828919 66-67 20.506457416684178 27.7262001488005 30.605128699906597 21.162213734608724 68-69 20.88231829300731 27.879129595346697 30.287177352250875 20.951374759395115 70-71 20.763700600771802 27.749428505863662 30.300010101260472 21.186860792104063 72-73 20.6928258862815 27.817432813687486 30.396196918958516 21.093544381072494 74-75 20.739678195328807 27.953810724503608 30.16364754071599 21.142863539451596 76-77 20.774078048265636 27.92036004398737 30.080389425760117 21.225172481986874 78-79 20.819782717507707 27.90599741683196 30.22317271244905 21.05104715321129 80-81 20.663190107852213 27.958113383152284 30.24391615533747 21.134780353658037 82-83 20.527914184460393 27.904376239104177 30.41727984707704 21.150429729358393 84-85 20.495725914835464 27.874484687720923 30.154765260139833 21.475024137303777 86-87 20.639055210031064 27.906046064108082 30.071762779038608 21.38313594682225 88-89 20.476313459464674 27.797349759938932 30.19041770678914 21.53591907380725 90-91 20.41501980761817 27.805359617512476 30.08333898592618 21.69628158894318 92-93 20.39736922659297 27.821629479822167 30.143472380490653 21.637528913094208 94-95 20.243087034225443 27.70731367984905 30.252296814796527 21.797302471128983 96-97 20.2675340777525 27.86423962848693 30.048256826744506 21.81996946701606 98-99 20.18261966833471 27.74183033580308 30.126568311267132 21.94898168459508 100 20.22683841460977 27.839557996913655 29.939765618961573 21.993837969515006 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4647.0 1 2655.5 2 2263.5 3 3712.5 4 2244.0 5 1287.5 6 3114.5 7 7839.5 8 13832.5 9 15468.0 10 10177.0 11 4999.5 12 4095.5 13 4431.5 14 4976.0 15 5477.0 16 5928.0 17 6569.5 18 7354.0 19 8221.0 20 9364.0 21 11086.5 22 13978.5 23 18514.0 24 23765.5 25 29641.5 26 38089.5 27 49407.5 28 64067.0 29 77589.5 30 85697.5 31 94017.0 32 102430.5 33 110521.5 34 118932.0 35 124404.5 36 126547.5 37 127070.5 38 127402.5 39 127241.0 40 127260.0 41 124935.0 42 119281.0 43 115198.0 44 112735.0 45 110038.0 46 108585.5 47 105907.5 48 102393.5 49 101122.5 50 100415.0 51 96295.5 52 90215.5 53 85876.5 54 83038.5 55 80048.5 56 73202.0 57 64779.5 58 59193.5 59 54883.0 60 48723.0 61 40756.5 62 34202.0 63 27763.5 64 21341.0 65 16942.0 66 13092.5 67 9516.0 68 7140.5 69 5540.5 70 4047.5 71 2976.5 72 2231.0 73 1661.0 74 1325.0 75 957.5 76 573.0 77 417.0 78 319.5 79 179.0 80 79.5 81 40.5 82 17.5 83 10.0 84 6.5 85 4.0 86 3.0 87 1.0 88 2.0 89 3.0 90 1.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0059967745348701775 2 0.0047707672966300525 3 0.0010127885881114078 4 0.0 5 0.044376131558039315 6 0.0 7 0.0 8 0.0 9 2.665233126608968E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 2.665233126608968E-5 20-21 0.0 22-23 3.997849689913452E-5 24-25 3.997849689913452E-5 26-27 1.1993549069740356E-4 28-29 1.7324015322958293E-4 30-31 1.0660932506435873E-4 32-33 0.0012926380664053494 34-35 8.528746005148698E-4 36-37 0.0011860287413409908 38-39 9.19505428680094E-4 40-41 2.665233126608968E-4 42-43 6.263297847531075E-4 44-45 1.1993549069740356E-4 46-47 9.328315943131388E-5 48-49 5.330466253217936E-5 50-51 3.5980647209221065E-4 52-53 5.463727909548385E-4 54-55 7.46265275450511E-4 56-57 0.001985598679323681 58-59 5.063942940557039E-4 60-61 0.0012793119007723046 62-63 0.001172702575707946 64-65 0.0015724875446992911 66-67 3.5980647209221065E-4 68-69 3.9978496899134515E-4 70-71 2.665233126608968E-4 72-73 1.7324015322958293E-4 74-75 5.463727909548385E-4 76-77 8.2622226924878E-4 78-79 9.328315943131387E-4 80-81 8.2622226924878E-4 82-83 0.001665770704130605 84-85 0.0011860287413409908 86-87 0.001918967851158457 88-89 0.0014658782196349323 90-91 0.0013725950602036185 92-93 0.001079419416276632 94-95 7.329391098174662E-4 96-97 2.3987098139480713E-4 98-99 3.065018095600313E-4 100 1.8656631886262775E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3752017.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.27347323893401 #Duplication Level Percentage of deduplicated Percentage of total 1 71.45261259352495 39.4943407004012 2 16.706343753547774 18.468352887643107 3 5.5633668733553 9.225198299783283 4 2.3993542271722412 5.304825666653123 5 1.2300979610605727 3.3995893365974426 6 0.7066724689585417 2.3436145081002833 7 0.45188212161405394 1.748396604933099 8 0.28895619820442897 1.2777290150941292 9 0.20094558551337105 0.9996264399019716 >10 0.9153604209692754 8.765691210438806 >50 0.04970032064952529 1.8897821877878842 >100 0.03142651060183261 3.370132085011775 >500 0.0020747277899224674 0.7685520000043313 >1k 9.649896305499054E-4 0.8996309179834039 >5k 9.649896305498984E-5 0.2977345497989114 >10k+ 1.4474844458248475E-4 1.7468035898673322 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27895 0.7434667806675715 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23360 0.6225984583758549 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14244 0.3796358065541814 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6021 0.16047368655312597 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5143 0.13707293970149922 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4612 0.12292055179920561 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4489 0.11964231505347657 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.6652331266089678E-5 0.0 5 0.0 0.0 0.0 2.6652331266089678E-5 0.0 6 0.0 0.0 0.0 2.6652331266089678E-5 0.0 7 0.0 0.0 0.0 2.6652331266089678E-5 0.0 8 0.0 0.0 0.0 2.6652331266089678E-5 0.0 9 0.0 0.0 0.0 2.6652331266089678E-5 0.0 10-11 0.0 0.0 0.0 3.997849689913452E-5 0.0 12-13 0.0 0.0 0.0 5.3304662532179356E-5 0.0 14-15 0.0 0.0 0.0 5.3304662532179356E-5 0.0 16-17 0.0 0.0 0.0 7.995699379826904E-5 0.0 18-19 0.0 0.0 0.0 1.0660932506435873E-4 0.0 20-21 0.0 0.0 0.0 1.998924844956726E-4 0.0 22-23 0.0 0.0 0.0 4.397634658904797E-4 0.0 24-25 0.0 0.0 0.0 9.594839255792284E-4 0.0 26-27 0.0 0.0 0.0 0.0015325090478001567 0.0 28-29 0.0 0.0 0.0 0.003411498402059479 0.0 30-31 0.0 0.0 0.0 0.007689197570266872 0.0 32-33 0.0 0.0 0.0 0.015045240999707624 0.0 34-35 0.0 0.0 0.0 0.0267189620942549 0.0 36-37 0.0 0.0 0.0 0.04628177324356472 0.0 38-39 0.0 0.0 0.0 0.0735870866256736 0.0 40-41 0.0 0.0 0.0 0.1017586007739304 0.0 42-43 0.0 0.0 0.0 0.12994344108782024 0.0 44-45 0.0 0.0 0.0 0.16625724243786744 0.0 46-47 0.0 0.0 0.0 0.2113396607744581 0.0 48-49 0.0 0.0 0.0 0.26073975677615535 0.0 50-51 0.0 0.0 0.0 0.3100998742809534 0.0 52-53 0.0 0.0 0.0 0.3579408089035844 0.0 54-55 0.0 0.0 0.0 0.4143238156969971 0.0 56-57 0.0 0.0 0.0 0.486818156740761 0.0 58-59 0.0 0.0 0.0 0.5678945484522058 0.0 60-61 0.0 0.0 0.0 0.6559271986241 0.0 62-63 0.0 0.0 0.0 0.7437999348083977 0.0 64-65 0.0 0.0 0.0 0.8385622986249796 0.0 66-67 0.0 0.0 0.0 0.9511817243898415 0.0 68-69 0.0 0.0 0.0 1.0821512802314062 0.0 70-71 0.0 0.0 0.0 1.2283659695571743 0.0 72-73 0.0 0.0 0.0 1.3832426665444215 0.0 74-75 0.0 0.0 0.0 1.5384391915068616 0.0 76-77 0.0 0.0 0.0 1.710360054338773 0.0 78-79 0.0 0.0 0.0 1.9133708615925782 0.0 80-81 0.0 0.0 0.0 2.1397157848698445 0.0 82-83 0.0 0.0 0.0 2.3804263146995335 0.0 84-85 0.0 0.0 0.0 2.6276133610268824 0.0 86-87 0.0 0.0 0.0 2.891258221911041 0.0 88 0.0 0.0 0.0 3.1029443629919586 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11125 0.0 63.40904 1 GTATCAA 21480 0.0 44.86086 1 GTGGTAT 3820 0.0 35.086613 1 ATCAACG 27790 0.0 34.60152 3 TATCAAC 28030 0.0 34.40774 2 TGGTATC 3760 0.0 34.39208 2 TCAACGC 28005 0.0 34.36946 4 CAACGCA 28970 0.0 33.25706 5 AACGCAG 30135 0.0 32.017986 6 CGCAGAG 33455 0.0 28.545582 8 ACGCAGA 33610 0.0 28.497837 7 GCAGAGT 35895 0.0 26.395676 9 CATGGGT 9920 0.0 25.785624 4 GTACATG 20215 0.0 25.730091 1 TATAACG 390 0.0 25.320267 2 TACATGG 20425 0.0 24.726109 2 ACATGGG 20460 0.0 24.176897 3 GAGTACT 24570 0.0 23.326677 12-13 CAGAGTA 35365 0.0 22.884201 10-11 ATGGGTA 4550 0.0 22.425325 5 >>END_MODULE