FastQCFastQC Report
Wed 25 May 2016
SRR2431392_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431392_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3742836
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA223670.5975949787807961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62150.051.492341
GTATCAA107650.039.083941
TCAACGC132900.030.6251874
ATCAACG132800.030.6169133
TATCAAC142000.029.229812
CAACGCA145400.028.0566925
AACGCAG149350.027.4694776
GTACATG243000.026.9205151
TACATGG242500.026.197982
CATGGGT106600.025.203834
TACCTGG30500.024.6868632
ACATGGG251400.024.4656123
ACGCAGA165500.024.277817
CGCAGAG168400.023.7474658
ATGGGTG59950.021.6621595
GCAGAGT185400.021.0889519
GTGGTAT28300.020.7873151
GTACCTG38250.020.4245031
ATGGGTA42450.020.3949175
ACCTGGG36200.020.2797133
Produced by FastQC (version 0.11.5)