##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431392_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3742836
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.465700340597344	31.0	31.0	34.0	26.0	34.0
2	30.615218780625174	33.0	31.0	34.0	26.0	34.0
3	30.667705985514726	34.0	31.0	34.0	26.0	34.0
4	33.83085018953542	37.0	35.0	37.0	28.0	37.0
5	33.98372036605397	37.0	35.0	37.0	30.0	37.0
6	33.91812385047061	37.0	35.0	37.0	30.0	37.0
7	33.89364348317693	37.0	35.0	37.0	29.0	37.0
8	33.843814957428	37.0	35.0	37.0	29.0	37.0
9	35.22486184273102	39.0	37.0	39.0	27.0	39.0
10-11	35.265444705565514	39.0	37.0	39.0	27.5	39.0
12-13	35.19522909900407	39.0	37.0	39.0	27.0	39.0
14-15	36.498356860947155	40.0	38.0	41.0	27.0	41.0
16-17	36.51564695861641	40.0	38.0	41.0	27.0	41.0
18-19	36.52513107707631	40.0	38.0	41.0	27.0	41.0
20-21	36.607299117567536	40.0	38.0	41.0	28.0	41.0
22-23	36.5677313940552	40.0	38.0	41.0	28.0	41.0
24-25	36.60728121670306	40.0	37.5	41.0	28.0	41.0
26-27	36.65262090030127	40.0	37.5	41.0	29.0	41.0
28-29	36.6215035871195	40.0	37.0	41.0	29.5	41.0
30-31	36.542442949677735	40.0	37.0	41.0	29.5	41.0
32-33	36.491419207253536	40.0	37.0	41.0	29.0	41.0
34-35	36.41463157883487	40.0	37.0	41.0	29.0	41.0
36-37	36.267481129282714	40.0	37.0	41.0	28.0	41.0
38-39	36.13679279562342	40.0	36.0	41.0	27.0	41.0
40-41	36.06278701498009	40.0	36.0	41.0	27.0	41.0
42-43	35.874781849912736	40.0	36.0	41.0	27.0	41.0
44-45	35.687164625968116	40.0	35.0	41.0	27.0	41.0
46-47	35.538849818693635	40.0	35.0	41.0	26.0	41.0
48-49	35.381987749396444	40.0	35.0	41.0	26.0	41.0
50-51	35.21553482439519	39.5	35.0	41.0	26.0	41.0
52-53	35.02880850242971	39.0	35.0	41.0	25.0	41.0
54-55	34.8495435012381	39.0	34.5	41.0	25.0	41.0
56-57	34.6297799315813	39.0	34.0	41.0	24.5	41.0
58-59	34.393896767050435	38.0	34.0	40.5	24.0	41.0
60-61	34.13810490227196	38.0	34.0	40.0	23.0	41.0
62-63	34.025662625880486	38.0	34.0	40.0	23.0	41.0
64-65	33.768391535188826	37.0	33.0	40.0	22.0	41.0
66-67	33.501968026384276	37.0	33.0	40.0	22.0	41.0
68-69	33.21404584651852	36.5	33.0	40.0	21.0	41.0
70-71	32.89994859512947	36.0	33.0	39.0	20.0	41.0
72-73	32.576772399325	35.5	32.0	39.0	18.5	41.0
74-75	32.301802563617535	35.0	32.0	39.0	18.0	40.0
76-77	32.05908955134556	35.0	32.0	37.5	17.5	40.0
78-79	31.729687995947458	35.0	32.0	37.0	14.0	39.0
80-81	31.41783984123269	35.0	32.0	37.0	11.5	39.0
82-83	31.105972182590953	35.0	32.0	36.0	7.5	39.0
84-85	30.81886128593398	35.0	31.0	36.0	4.5	37.0
86-87	30.54676734433462	35.0	31.0	35.5	2.0	37.0
88-89	30.288899780807924	35.0	31.0	35.0	2.0	37.0
90-91	30.057744715504498	35.0	31.0	35.0	2.0	36.0
92-93	29.83115477140863	35.0	31.0	35.0	2.0	36.0
94-95	29.614375569755126	34.5	31.0	35.0	2.0	36.0
96-97	29.41347496924792	34.0	30.0	35.0	2.0	35.5
98-99	29.192613702550688	34.0	30.0	35.0	2.0	35.0
100	29.016658491047966	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.32858084356865547
1101	2	0.37971229855201827
1101	3	0.40466693943268695
1101	4	0.48675060462334585
1101	5	0.4772052739084458
1101	6	0.4696705616158212
1101	7	0.49357902839464884
1101	8	0.4114743688424909
1101	9	0.46962425297911636
1101	10-11	0.5152856461043172
1101	12-13	0.40644187499323436
1101	14-15	0.4407960769121786
1101	16-17	0.3831109019382808
1101	18-19	0.40427095688369974
1101	20-21	0.32440228118954906
1101	22-23	0.45431579188730353
1101	24-25	0.4481917822533461
1101	26-27	0.4557849744224569
1101	28-29	0.42075479830359086
1101	30-31	0.4800605362179411
1101	32-33	0.47199630201854603
1101	34-35	0.46314175079377407
1101	36-37	0.46170731430611767
1101	38-39	0.5948333778184036
1101	40-41	0.4778279254319102
1101	42-43	0.4651555695788687
1101	44-45	0.47195323404032763
1101	46-47	0.44112965060988074
1101	48-49	0.4332168051633545
1101	50-51	0.47069977225625337
1101	52-53	0.47928243176524177
1101	54-55	0.4664634723732277
1101	56-57	0.31246381132178414
1101	58-59	0.36658663895415344
1101	60-61	0.33966282253136626
1101	62-63	0.3234132807021268
1101	64-65	0.48589021810425237
1101	66-67	0.48811244850735136
1101	68-69	0.42499203004835806
1101	70-71	0.3747741691758222
1101	72-73	0.29654104452718144
1101	74-75	0.37986697509159484
1101	76-77	0.41586950112392174
1101	78-79	0.4488539273846932
1101	80-81	0.43734217272448817
1101	82-83	0.3811593891861804
1101	84-85	0.3458032402376787
1101	86-87	0.3932903632198368
1101	88-89	0.4181623574056168
1101	90-91	0.3126510231002726
1101	92-93	0.3520229142280904
1101	94-95	0.3420089423778627
1101	96-97	0.32749781885351226
1101	98-99	0.35715318734280643
1101	100	0.3841157118627798
1102	1	0.2938535502529227
1102	2	0.29587791663009
1102	3	0.30341650443873647
1102	4	0.305382155079883
1102	5	0.4201161153386508
1102	6	0.4082466537958709
1102	7	0.3289904360074587
1102	8	0.27764910107954677
1102	9	0.4392894319204359
1102	10-11	0.4560169606974682
1102	12-13	0.38487052493771046
1102	14-15	0.4385283084449654
1102	16-17	0.4496795453647593
1102	18-19	0.3953929374733036
1102	20-21	0.3416443366809716
1102	22-23	0.4562860383010161
1102	24-25	0.4022341189027614
1102	26-27	0.358842429528309
1102	28-29	0.3968105217192459
1102	30-31	0.3667883183840175
1102	32-33	0.3545872917057338
1102	34-35	0.4074750224454249
1102	36-37	0.3656897184555348
1102	38-39	0.3836673592697153
1102	40-41	0.3665243659385524
1102	42-43	0.38678015460845216
1102	44-45	0.3119419518162587
1102	46-47	0.19677794350027966
1102	48-49	0.16857877536833854
1102	50-51	0.16535121091244065
1102	52-53	0.20670396714199057
1102	54-55	0.20246833601988357
1102	56-57	0.14290033810912206
1102	58-59	0.13907927858031144
1102	60-61	0.07960320764600937
1102	62-63	0.13379803630457587
1102	64-65	0.14862532197805223
1102	66-67	0.11616886791332348
1102	68-69	0.04702157373279192
1102	70-71	0.055554410231515305
1102	72-73	0.07628261669198366
1102	74-75	0.036043267783142596
1102	76-77	0.056274984533388306
1102	78-79	0.06952898276011155
1102	80-81	0.048205352626936815
1102	82-83	0.13013031778088902
1102	84-85	-0.02786429733751561
1102	86-87	-0.05935481428531375
1102	88-89	-0.10417444391124064
1102	90-91	-0.10185830372241966
1102	92-93	-0.1315782971264916
1102	94-95	-0.06396373171638103
1102	96-97	-0.04945889441504292
1102	98-99	-0.16316020163292677
1102	100	-0.1930962669039893
1103	1	-0.3067394947523354
1103	2	-0.11508331938459193
1103	3	-0.1772169406806512
1103	4	-0.049232775732683365
1103	5	-0.10003811082204805
1103	6	-0.1515471691631376
1103	7	-0.18787878257907664
1103	8	-0.17539069392881146
1103	9	-0.12418306195613837
1103	10-11	-0.23783563333169155
1103	12-13	-0.2227044620021985
1103	14-15	-0.21187328054348598
1103	16-17	-0.275880246390642
1103	18-19	-0.27784632879115634
1103	20-21	-0.35040410486153917
1103	22-23	-0.32437345806903295
1103	24-25	-0.25538986919912077
1103	26-27	-0.28626596572843255
1103	28-29	-0.32126401518186753
1103	30-31	-0.32726958431111086
1103	32-33	-0.3038840856886722
1103	34-35	-0.3297359844147394
1103	36-37	-0.3990293070898474
1103	38-39	-0.3536866537945116
1103	40-41	-0.33167654709576055
1103	42-43	-0.406711443518617
1103	44-45	-0.5034079550746426
1103	46-47	-0.5927449395599282
1103	48-49	-0.5596676033010581
1103	50-51	-0.7317125098953454
1103	52-53	-0.7075120900770315
1103	54-55	-0.6764759818439714
1103	56-57	-0.6686951218057189
1103	58-59	-0.6769851079816576
1103	60-61	-0.5796360613751901
1103	62-63	-0.5493944617622546
1103	64-65	-0.5789108844360698
1103	66-67	-0.5936188902042616
1103	68-69	-0.5349259088755787
1103	70-71	-0.5077331059815933
1103	72-73	-0.5415705879564356
1103	74-75	-0.5332440995656818
1103	76-77	-0.48748677471170154
1103	78-79	-0.4693569541131595
1103	80-81	-0.4506092120897449
1103	82-83	-0.4618901083293565
1103	84-85	-0.5095307641400879
1103	86-87	-0.5334657292079967
1103	88-89	-0.5888954887731472
1103	90-91	-0.582528723698907
1103	92-93	-0.6624957560130689
1103	94-95	-0.615660233267743
1103	96-97	-0.5384517930370869
1103	98-99	-0.651566671440353
1103	100	-0.6603779636260825
1104	1	-0.17174396959313398
1104	2	-0.10803474001756541
1104	3	-0.08761673210698007
1104	4	-0.17537492639414154
1104	5	-0.09232913988408598
1104	6	-0.11721423688010191
1104	7	-0.13170298634289423
1104	8	-0.11237165882968725
1104	9	-0.09256461419276718
1104	10-11	-0.10970539448319983
1104	12-13	-0.178376159339237
1104	14-15	-0.18029837734933096
1104	16-17	-0.20584252152215754
1104	18-19	-0.32747382667067626
1104	20-21	-0.2608747851955684
1104	22-23	-0.1806841874371301
1104	24-25	-0.20735026909373744
1104	26-27	-0.27597266244291063
1104	28-29	-0.20888707330389167
1104	30-31	-0.24946510424221913
1104	32-33	-0.2979961445075503
1104	34-35	-0.2849601976716869
1104	36-37	-0.4480121664890433
1104	38-39	-0.3354802085103543
1104	40-41	-0.35005338716336354
1104	42-43	-0.3745495141296047
1104	44-45	-0.3866662376575718
1104	46-47	-0.539822431695427
1104	48-49	-0.5878167940118217
1104	50-51	-0.5666076692939157
1104	52-53	-0.584176156451889
1104	54-55	-0.5345215461107315
1104	56-57	-0.5434031221056443
1104	58-59	-0.6472381480185874
1104	60-61	-0.5899881807849923
1104	62-63	-0.5368010008770412
1104	64-65	-0.476080521832948
1104	66-67	-0.40621321097432883
1104	68-69	-0.3735753943548801
1104	70-71	-0.4455684180819688
1104	72-73	-0.5185358547993459
1104	74-75	-0.452432223799093
1104	76-77	-0.4647097662442441
1104	78-79	-0.4328896156356521
1104	80-81	-0.44181661425376717
1104	82-83	-0.5172534853517199
1104	84-85	-0.42339953745586456
1104	86-87	-0.42120934318710823
1104	88-89	-0.4785430254094045
1104	90-91	-0.4791225821948757
1104	92-93	-0.5137633982707008
1104	94-95	-0.5214048832361335
1104	96-97	-0.5106207941191627
1104	98-99	-0.4763942989217256
1104	100	-0.5328414924697142
1105	1	-0.3494559594574653
1105	2	-0.28542602235511794
1105	3	-0.20221480786182155
1105	4	-0.20524375646840554
1105	5	-0.19196937007109227
1105	6	-0.21550527896064864
1105	7	-0.14582615773534968
1105	8	-0.2779062717094334
1105	9	-0.3933895056940173
1105	10-11	-0.4179453135401161
1105	12-13	-0.35249274281896703
1105	14-15	-0.3924439777455575
1105	16-17	-0.37249253027867013
1105	18-19	-0.31786447005594454
1105	20-21	-0.2806166320549863
1105	22-23	-0.3539436581602615
1105	24-25	-0.31277387256569966
1105	26-27	-0.3295194290980845
1105	28-29	-0.36755602761127903
1105	30-31	-0.339160459455897
1105	32-33	-0.37081255586694084
1105	34-35	-0.36560640828786717
1105	36-37	-0.45896992449167584
1105	38-39	-0.486429196311434
1105	40-41	-0.48794797782344546
1105	42-43	-0.5370388763266192
1105	44-45	-0.5630565403595114
1105	46-47	-0.6268788592543757
1105	48-49	-0.7123351854391942
1105	50-51	-0.6029999695768566
1105	52-53	-0.5354260127902393
1105	54-55	-0.6360552710246665
1105	56-57	-0.6736138326972068
1105	58-59	-0.6425586434734072
1105	60-61	-0.686663268760654
1105	62-63	-0.5989133880684463
1105	64-65	-0.5568178549802028
1105	66-67	-0.6463704725917623
1105	68-69	-0.5728305087095507
1105	70-71	-0.6126847115019913
1105	72-73	-0.5212311867535249
1105	74-75	-0.5127924177559997
1105	76-77	-0.533303619714836
1105	78-79	-0.5407880304319796
1105	80-81	-0.5095492503492345
1105	82-83	-0.45339738780409533
1105	84-85	-0.4484789276190142
1105	86-87	-0.5313389601988376
1105	88-89	-0.539392968765668
1105	90-91	-0.5704423059814161
1105	92-93	-0.6123492887813704
1105	94-95	-0.593863132424783
1105	96-97	-0.6035915664989098
1105	98-99	-0.6990119441294489
1105	100	-0.5825132598089908
1106	1	-0.022776433776709126
1106	2	-0.012502500209922829
1106	3	0.021700562254149958
1106	4	0.10601560159257417
1106	5	0.1359502650843325
1106	6	0.08211899821899493
1106	7	0.13779896606261133
1106	8	0.029925213972603615
1106	9	0.1179730173350606
1106	10-11	0.08879748221720263
1106	12-13	0.16187520868417948
1106	14-15	0.13498798662192968
1106	16-17	0.1593865763526381
1106	18-19	0.08654280393955815
1106	20-21	0.08537194126003556
1106	22-23	0.1626376145360382
1106	24-25	0.11061721983817563
1106	26-27	0.010204607940138999
1106	28-29	0.050175035294053316
1106	30-31	0.10150038205075873
1106	32-33	0.034317338811611364
1106	34-35	0.08669643347437272
1106	36-37	0.039850267621453384
1106	38-39	0.013669842057950632
1106	40-41	0.0029565992401217045
1106	42-43	-0.007005158021563318
1106	44-45	-0.05303462945144588
1106	46-47	-0.04833331225374593
1106	48-49	0.02497698776080881
1106	50-51	-0.010485220314322419
1106	52-53	-0.0700111698139736
1106	54-55	-0.002226146822032149
1106	56-57	0.03706813433260692
1106	58-59	-0.0737392899916216
1106	60-61	-0.13439703392257485
1106	62-63	-0.07702524285476642
1106	64-65	0.04038850424154816
1106	66-67	0.04130776984990092
1106	68-69	0.0918261715882025
1106	70-71	-0.006509256511201045
1106	72-73	-0.029068462985378574
1106	74-75	-0.021560774464525423
1106	76-77	-0.013621208077296387
1106	78-79	-0.04447461392771501
1106	80-81	-0.012714218454618731
1106	82-83	-0.0032413757490985518
1106	84-85	0.03703478067571808
1106	86-87	0.021902483099033532
1106	88-89	-0.07891207358660068
1106	90-91	-0.07320692745251023
1106	92-93	-0.07573770555186954
1106	94-95	-0.10967219869529643
1106	96-97	0.004584024270286591
1106	98-99	-0.04525560780366433
1106	100	-0.017036384735778398
1107	1	-0.434885634505374
1107	2	-0.5165258854794068
1107	3	-0.4304112175885102
1107	4	-0.39331438236612115
1107	5	-0.2987189537762873
1107	6	-0.4138203626426957
1107	7	-0.44041999290016776
1107	8	-0.45133410356518056
1107	9	-0.43390214833718943
1107	10-11	-0.4739070802745289
1107	12-13	-0.5547224645121247
1107	14-15	-0.5731902796533603
1107	16-17	-0.6076273697503893
1107	18-19	-0.5690316155481199
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2216	78-79	0.6594793129974796
2216	80-81	0.6282333046922766
2216	82-83	0.5907274544961076
2216	84-85	0.6524595636145065
2216	86-87	0.6013513054966211
2216	88-89	0.6343840176255995
2216	90-91	0.706021385074763
2216	92-93	0.6730580734927436
2216	94-95	0.6084143877035935
2216	96-97	0.6485285095309692
2216	98-99	0.5961889566484722
2216	100	0.6165509573028238
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	110047.0
3	21809.0
4	15625.0
5	13160.0
6	9000.0
7	7622.0
8	8281.0
9	8546.0
10	9022.0
11	9655.0
12	10997.0
13	12134.0
14	12714.0
15	12454.0
16	12207.0
17	12173.0
18	12433.0
19	11770.0
20	11541.0
21	12715.0
22	14892.0
23	22786.0
24	34755.0
25	42763.0
26	47724.0
27	37865.0
28	36584.0
29	44062.0
30	52882.0
31	60714.0
32	77163.0
33	102932.0
34	146682.0
35	230002.0
36	388636.0
37	675725.0
38	943767.0
39	447335.0
40	1662.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.48878563705076	21.509281959596176	14.152499277356087	21.84943312599698
2	14.774759150823492	22.54506730555897	40.0330312030054	22.647142340612135
3	17.150901033530587	29.921342251411424	29.967435167449512	22.960321547608466
4	11.795179239576697	19.48120353072793	38.16620007815135	30.557417151544026
5	12.102489152285582	41.18295630666442	33.81858285084092	12.895971690209084
6	27.47679783832403	40.48875138667524	18.68956148041436	13.34488929458637
7	25.144766081488257	36.1452235682416	22.253294720132278	16.456715630137868
8	24.75785531552664	37.45254889211906	20.328309934502165	17.461285857852136
9	25.6876638090421	18.982360218957417	20.647157328325985	34.682818643674494
10-11	22.803058599631086	29.78660174223285	28.09557014263893	19.314769515497137
12-13	23.517482497850708	28.007943995261197	27.90429934787959	20.57027415900851
14-15	21.469472753797284	29.123204492933468	25.56680681238952	23.840515940879726
16-17	20.54108637015122	32.16760716297531	26.252399141161604	21.038907325711868
18-19	20.740103327745892	31.01477995564182	27.996533372439615	20.248583344172673
20-21	22.68117629538448	29.66277318516233	27.288223597615413	20.367826921837782
22-23	22.314542106208773	29.817838672005337	27.186267464045244	20.681351757740646
24-25	21.590258463788402	29.979526892759818	27.35268620133961	21.077528442112172
26-27	21.379430753472402	30.01660099616672	27.4354923362433	21.168475914117586
28-29	21.25925947711125	29.99038559743245	27.591824763342927	21.158530162113372
30-31	21.422745440493497	30.12748209198598	27.414966595726675	21.034805871793846
32-33	21.223866442379073	30.052443245083037	27.577668580989716	21.146021731548174
34-35	21.552498010438896	29.7882716626207	27.546768555723688	21.112461771216715
36-37	21.502687553504728	29.88468849234017	27.37106421449943	21.241559739655663
38-39	21.42243973054601	29.88831218523003	27.41352219230636	21.275725891917595
40-41	21.445150457408168	29.722293150307678	27.547375133519335	21.28518125876482
42-43	21.390667164888466	29.764403032914053	27.612713212080052	21.232216590117428
44-45	21.417085261852797	29.73423701492529	27.56723561918461	21.2814421040373
46-47	21.42363057065973	29.749232075705784	27.484604080480178	21.342533273154306
48-49	21.383693626206078	29.673092527231372	27.438857348676393	21.50435649788616
50-51	21.34559970843918	29.721515832830086	27.43191844960449	21.50096600912625
52-53	21.399533304402162	29.775053470523765	27.392242756578966	21.433170468495106
54-55	21.441358429903683	29.73633680736137	27.442205523381034	21.380099239353907
56-57	21.385453580964267	29.62862846322621	27.376004055526053	21.60991390028347
58-59	21.481915550865466	29.447846362968725	27.501513115067738	21.568724971098067
60-61	21.56320794130918	29.79775731546316	27.256845869750496	21.382188873477165
62-63	21.34923535594135	29.66809322510846	27.47959554434652	21.50307587460367
64-65	21.561187757836304	29.43092095896423	27.407306599125512	21.60058468407395
66-67	21.32953827351515	29.57006389224105	27.31603525068878	21.784362583555016
68-69	21.507084616080796	29.49086829075006	27.40173170740511	21.60031538576403
70-71	21.42743134704013	29.464226991493277	27.440143046048824	21.668198615417765
72-73	21.491523502441765	29.565286669542452	27.41942404757955	21.523765780436236
74-75	21.49139399915353	29.477220573337693	27.375042076188176	21.656343351320597
76-77	21.452741205656967	29.40881571300785	27.237179719418975	21.901263361916204
78-79	21.46873471413196	29.49668043918225	27.35647509871521	21.678109747970584
80-81	21.230154406349147	29.568415450180613	27.45405664770317	21.74737349576707
82-83	21.306156543911563	29.58986678103821	27.53698135062882	21.56699532442141
84-85	21.403100221750005	29.345389602697686	27.436970509361874	21.814539666190438
86-87	21.46225046638611	29.525201962498198	27.31910275254036	21.693444818575337
88-89	21.398249454124244	29.412194888474495	27.35219909183815	21.837356565563116
90-91	21.35722271918617	29.354267584847477	27.260726566783376	22.027783129182982
92-93	21.3745165424948	29.413212392981634	27.351797181254923	21.860473883268643
94-95	21.311128058867684	29.298382930029454	27.455071549313846	21.93541746178901
96-97	21.349975242468737	29.458227146873657	27.22118635095378	21.97061125970383
98-99	21.182663530423422	29.501105769063653	27.31495581068207	22.001274889830853
100	21.28308120898818	29.52466064092557	27.228760760108706	21.963497389977547
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20118.0
1	15433.0
2	9883.0
3	7641.0
4	5197.5
5	3704.5
6	3113.0
7	3172.0
8	3694.5
9	3850.5
10	3015.0
11	2178.0
12	2064.0
13	2091.5
14	2120.5
15	2202.0
16	2413.0
17	2927.5
18	3559.0
19	4431.0
20	6096.0
21	8404.0
22	11310.0
23	15409.0
24	21278.5
25	27404.5
26	34903.5
27	44345.0
28	55083.0
29	67184.0
30	75883.5
31	82718.5
32	89336.0
33	96357.5
34	102722.5
35	106741.0
36	108959.0
37	111962.0
38	114964.0
39	115310.0
40	116218.0
41	116801.5
42	115293.5
43	113584.5
44	112496.5
45	111782.0
46	113684.5
47	113211.0
48	110650.5
49	110995.5
50	111419.5
51	108202.0
52	103348.0
53	99250.5
54	96352.5
55	94189.5
56	85866.5
57	75084.5
58	68690.5
59	64173.0
60	57779.5
61	48855.0
62	41055.5
63	33918.0
64	27367.5
65	22604.5
66	18225.5
67	14135.0
68	10971.0
69	8546.5
70	6488.5
71	4920.5
72	3869.5
73	2960.5
74	2337.5
75	1891.0
76	1384.0
77	1046.5
78	830.0
79	647.0
80	474.0
81	337.5
82	262.0
83	224.5
84	185.0
85	138.5
86	130.5
87	120.0
88	92.0
89	84.5
90	76.0
91	62.0
92	56.0
93	52.5
94	48.0
95	38.0
96	32.0
97	33.0
98	35.5
99	42.0
100	44.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17516129480426074
2	0.17016508337528014
3	0.18445905724963638
4	0.1735582323136787
5	0.18771861764715314
6	0.07414164018941787
7	0.07579813809635261
8	0.06430952358051488
9	0.058778957988006954
10-11	0.07195078811895578
12-13	0.07086872093781293
14-15	0.06846412720193992
16-17	0.06505761940945315
18-19	0.06950611782081823
20-21	0.07088207979190111
22-23	0.06893168709502634
24-25	0.06849084491011628
26-27	0.07211109436801398
28-29	0.06984008917302281
30-31	0.07535729591144255
32-33	0.07207101780574944
34-35	0.0718572761403385
36-37	0.08273138336811979
38-39	0.07754814798190464
40-41	0.07323323811142139
42-43	0.07663974590390817
44-45	0.08668560417822208
46-47	0.07705387038064185
48-49	0.08222374691276882
50-51	0.07920464588883937
52-53	0.07814929641587288
54-55	0.07985922973916036
56-57	0.07358056831771416
58-59	0.08107488546118505
60-61	0.07744127714919917
62-63	0.0694393235503773
64-65	0.07204430009757308
66-67	0.07241834801204221
68-69	0.07092215635416566
70-71	0.06684770585726972
72-73	0.06426944701825034
74-75	0.06938588813402458
76-77	0.07415499904350605
78-79	0.07100230947869476
80-81	0.07832296151901927
82-83	0.07956533494922031
84-85	0.0811951151479787
86-87	0.07772181308505101
88-89	0.07945846411651485
90-91	0.07760158339825737
92-93	0.06919886417679
94-95	0.06902519907364363
96-97	0.06839733293149901
98-99	0.07761494225234555
100	0.08584399637066653
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3742836.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.36887054166114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.71609264361044	38.27406864050623
2	15.100280181899592	16.117697963412216
3	5.611125326622614	8.983782634486753
4	2.619792477825011	5.592614623802428
5	1.4367890695588132	3.833990492447904
6	0.8972398903732881	2.8730807732487746
7	0.5764238363063113	2.153416236787144
8	0.3938348547927968	1.681481710418455
9	0.27678183852992033	1.329438069790765
>10	1.2672486083109489	11.723234643840454
>50	0.06928738399193393	2.5553508293483693
>100	0.03255326526112334	3.0321336293091923
>500	0.0018004397297205444	0.6543554248603275
>1k	7.001709750804602E-4	0.598357783170612
>5k	0.0	0.0
>10k+	5.0012212505746786E-5	0.5969965445703014
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22367	0.5975949787807961	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	1.3358854088183398E-5	0.0	1.3358854088183398E-5	0.0
14-15	0.0	2.6717708176366797E-5	0.0	2.6717708176366797E-5	0.0
16-17	0.0	2.6717708176366797E-5	0.0	9.351197861728379E-5	0.0
18-19	0.0	2.6717708176366797E-5	0.0	1.2022968679365059E-4	0.0
20-21	0.0	2.6717708176366797E-5	0.0	1.8702395723456757E-4	0.0
22-23	0.0	2.6717708176366797E-5	0.0	4.4084218491005216E-4	0.0
24-25	0.0	2.6717708176366797E-5	0.0	0.0010553494729664886	0.0
26-27	0.0	2.6717708176366797E-5	0.0	0.0013492442629065233	0.0
28-29	0.0	2.6717708176366797E-5	0.0	0.002591617693107579	0.0
30-31	0.0	2.6717708176366797E-5	0.0	0.005490489030243377	0.0
32-33	0.0	2.6717708176366797E-5	0.0	0.011047772330927672	0.0
34-35	0.0	2.6717708176366797E-5	0.0	0.019089802492014078	0.0
36-37	0.0	2.6717708176366797E-5	0.0	0.03282270449466661	0.0
38-39	0.0	2.6717708176366797E-5	0.0	0.054757942907463754	0.0
40-41	0.0	2.6717708176366797E-5	0.0	0.07849662662216565	0.0
42-43	0.0	2.6717708176366797E-5	0.0	0.10065896555446191	0.0
44-45	0.0	2.6717708176366797E-5	0.0	0.12653506592327315	0.0
46-47	0.0	2.6717708176366797E-5	0.0	0.15993220114373163	0.0
48-49	0.0	2.6717708176366797E-5	0.0	0.19735035144473334	0.0
50-51	0.0	2.6717708176366797E-5	0.0	0.23921700015710012	0.0
52-53	0.0	2.6717708176366797E-5	0.0	0.2813641848053188	0.0
54-55	0.0	5.343541635273359E-5	0.0	0.32500756111141393	0.0
56-57	0.0	5.343541635273359E-5	0.0	0.379792221727054	0.0
58-59	0.0	5.343541635273359E-5	0.0	0.44643954477300096	0.0
60-61	0.0	5.343541635273359E-5	0.0	0.5197662948630397	0.0
62-63	0.0	5.343541635273359E-5	0.0	0.5948964902549831	0.0
64-65	0.0	5.343541635273359E-5	0.0	0.6712690590771276	0.0
66-67	0.0	5.343541635273359E-5	0.0	0.7632447694742703	0.0
68-69	0.0	5.343541635273359E-5	0.0	0.8711175162363513	0.0
70-71	0.0	5.343541635273359E-5	0.0	0.9942193566589612	0.0
72-73	0.0	5.343541635273359E-5	0.0	1.124441466310573	0.0
74-75	0.0	5.343541635273359E-5	0.0	1.2540757863823047	0.0
76-77	0.0	5.343541635273359E-5	0.0	1.394383830870495	0.0
78-79	0.0	5.343541635273359E-5	0.0	1.5629859283174576	0.0
80-81	0.0	5.343541635273359E-5	0.0	1.751091952733168	0.0
82-83	0.0	5.343541635273359E-5	0.0	1.9544003531012313	0.0
84-85	0.0	5.343541635273359E-5	0.0	2.1551572123384517	0.0
86-87	0.0	5.343541635273359E-5	0.0	2.369299643372031	0.0
88	0.0	5.343541635273359E-5	0.0	2.546651790246754	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6215	0.0	51.49234	1
GTATCAA	10765	0.0	39.08394	1
TCAACGC	13290	0.0	30.625187	4
ATCAACG	13280	0.0	30.616913	3
TATCAAC	14200	0.0	29.22981	2
CAACGCA	14540	0.0	28.056692	5
AACGCAG	14935	0.0	27.469477	6
GTACATG	24300	0.0	26.920515	1
TACATGG	24250	0.0	26.19798	2
CATGGGT	10660	0.0	25.20383	4
TACCTGG	3050	0.0	24.686863	2
ACATGGG	25140	0.0	24.465612	3
ACGCAGA	16550	0.0	24.27781	7
CGCAGAG	16840	0.0	23.747465	8
ATGGGTG	5995	0.0	21.662159	5
GCAGAGT	18540	0.0	21.088951	9
GTGGTAT	2830	0.0	20.787315	1
GTACCTG	3825	0.0	20.424503	1
ATGGGTA	4245	0.0	20.394917	5
ACCTGGG	3620	0.0	20.279713	3
>>END_MODULE