FastQCFastQC Report
Wed 25 May 2016
SRR2431392_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431392_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3742836
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT358580.9580435797881606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284250.7594508549132262No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192030.5130601501107717No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65080.1738788448117951No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58870.15728714803427132No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54560.14577181581025725No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52590.140508427299513No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44470.11881364826030316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118700.065.41191
GTATCAA238400.046.042091
TATCAAC312500.035.0187762
ATCAACG312950.034.8449443
TCAACGC315350.034.5797544
CAACGCA327600.033.315425
AACGCAG334850.032.7025536
GTGGTAT35900.031.8326131
TGGTATC36550.030.236752
ACGCAGA373800.029.232087
CGCAGAG377750.028.8019968
TACCTGG29700.027.551642
GCAGAGT402750.026.7224319
GTACATG234100.025.8545861
TACATGG236250.025.141212
CATGGGT105050.025.0672074
TAACGCA3850.024.427744
TATAACG3500.024.1857242
ACATGGG240200.024.118583
GAGTACT262900.023.81166312-13
Produced by FastQC (version 0.11.5)