##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431392_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3742836
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.633321096622986	34.0	31.0	34.0	31.0	34.0
2	32.73171306463868	34.0	31.0	34.0	31.0	34.0
3	32.829552777626375	34.0	31.0	34.0	31.0	34.0
4	36.30483729450075	37.0	37.0	37.0	35.0	37.0
5	36.21545320179671	37.0	37.0	37.0	35.0	37.0
6	36.26210419051222	37.0	37.0	37.0	35.0	37.0
7	36.21493728285183	37.0	37.0	37.0	35.0	37.0
8	36.228626100635985	37.0	37.0	37.0	35.0	37.0
9	38.016676926266605	39.0	39.0	39.0	35.0	39.0
10-11	37.99099305980812	39.0	39.0	39.0	35.0	39.0
12-13	37.96776829121019	39.0	38.5	39.0	35.0	39.0
14-15	39.40424026059384	41.0	39.0	41.0	36.0	41.0
16-17	39.4363479992177	41.0	39.0	41.0	36.0	41.0
18-19	39.519680931785416	41.0	39.5	41.0	36.0	41.0
20-21	39.51856840641695	41.0	40.0	41.0	36.0	41.0
22-23	39.45424939805004	41.0	39.0	41.0	36.0	41.0
24-25	39.39935853454439	41.0	39.0	41.0	36.0	41.0
26-27	39.27574184388523	41.0	39.0	41.0	35.5	41.0
28-29	39.1919380117109	41.0	39.0	41.0	35.0	41.0
30-31	38.99605352732527	41.0	38.5	41.0	35.0	41.0
32-33	38.76054828477657	41.0	38.0	41.0	35.0	41.0
34-35	38.62764932794277	41.0	38.0	41.0	35.0	41.0
36-37	38.48843590795856	41.0	38.0	41.0	35.0	41.0
38-39	38.347105510367	40.0	38.0	41.0	35.0	41.0
40-41	38.198441636235195	40.0	38.0	41.0	34.0	41.0
42-43	38.017145154102394	40.0	38.0	41.0	34.0	41.0
44-45	37.750428151273525	40.0	37.5	41.0	33.0	41.0
46-47	37.35266599979267	40.0	37.0	41.0	33.0	41.0
48-49	36.99280077994334	40.0	37.0	41.0	31.5	41.0
50-51	36.81398116294703	40.0	36.5	41.0	31.0	41.0
52-53	36.74086267739222	40.0	36.0	41.0	31.0	41.0
54-55	36.586291250805544	40.0	36.0	41.0	31.0	41.0
56-57	36.41377394040241	40.0	35.0	41.0	31.0	41.0
58-59	36.219253127842094	39.5	35.0	41.0	30.5	41.0
60-61	36.049819308139604	39.0	35.0	41.0	30.0	41.0
62-63	35.87577321047463	39.0	35.0	41.0	30.0	41.0
64-65	35.633362108305036	38.5	35.0	41.0	30.0	41.0
66-67	35.36330726219369	38.0	35.0	41.0	29.5	41.0
68-69	35.06473994585924	37.0	35.0	40.0	29.0	41.0
70-71	34.76215308391818	37.0	35.0	40.0	29.0	41.0
72-73	34.44305360961581	36.0	35.0	39.0	29.0	41.0
74-75	34.110927783103506	36.0	35.0	39.0	28.5	41.0
76-77	33.790965326827035	35.0	34.5	38.5	28.0	40.5
78-79	33.484422908190474	35.0	34.0	37.0	28.0	39.5
80-81	33.23378114884008	35.0	34.0	37.0	28.0	39.0
82-83	33.05236537214027	35.0	34.0	36.5	29.0	39.0
84-85	32.80837565418308	35.0	34.0	36.0	28.0	38.0
86-87	32.583649403821056	35.0	34.0	36.0	28.0	37.0
88-89	32.36331474315199	35.0	34.0	35.5	27.0	37.0
90-91	32.18751583024209	35.0	34.0	35.0	27.0	36.5
92-93	32.03442269444881	35.0	34.0	35.0	27.0	36.0
94-95	31.902779603487836	35.0	34.0	35.0	26.0	36.0
96-97	31.76650312757492	35.0	34.0	35.0	26.0	36.0
98-99	31.65690201227091	35.0	34.0	35.0	25.5	36.0
100	31.55646440292869	35.0	34.0	35.0	25.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.019195911237915197
1101	2	9.842374469570814E-4
1101	3	0.031195394372772967
1101	4	0.043885129638951526
1101	5	0.029671906962398964
1101	6	0.010174066261811276
1101	7	0.025488405447653406
1101	8	0.04221364040355269
1101	9	-0.022126676407751233
1101	10-11	0.06502456556261649
1101	12-13	0.06016930196442871
1101	14-15	0.03685614923517022
1101	16-17	0.01652272434755986
1101	18-19	0.015194080533440513
1101	20-21	0.04233817092946168
1101	22-23	0.022128744353430818
1101	24-25	0.07413381739090852
1101	26-27	0.08826045186262377
1101	28-29	0.028455851297721324
1101	30-31	0.04323905218296176
1101	32-33	0.04565128467993418
1101	34-35	0.06619041117925661
1101	36-37	0.07933488599447713
1101	38-39	0.0883348177952854
1101	40-41	0.04633443589754904
1101	42-43	0.06821751865862069
1101	44-45	0.1179631228023581
1101	46-47	0.0909432923802953
1101	48-49	0.06645128372855424
1101	50-51	-0.05882904690901114
1101	52-53	0.0035497566986464335
1101	54-55	0.059212583649923545
1101	56-57	0.04276405850963272
1101	58-59	0.0029139449030637365
1101	60-61	-0.04261623277566429
1101	62-63	-0.015256847312372201
1101	64-65	-0.029814743545763633
1101	66-67	0.009380122267472757
1101	68-69	0.02385086340539999
1101	70-71	0.019751215314393278
1101	72-73	0.028797049873034553
1101	74-75	0.026311550764184233
1101	76-77	-0.042023633936636884
1101	78-79	-0.007430166049836373
1101	80-81	8.844534924463687E-4
1101	82-83	-0.00260375136081592
1101	84-85	0.013028141635246016
1101	86-87	0.03937159310563487
1101	88-89	1.9879099675534917E-4
1101	90-91	-0.013326582564857858
1101	92-93	-0.03545404812088293
1101	94-95	0.0013906202141811264
1101	96-97	0.02445629789350079
1101	98-99	-0.044791385917449134
1101	100	0.031988888014105044
1102	1	-0.018797012860474638
1102	2	0.008959678931290682
1102	3	0.013059875114279862
1102	4	0.041913157058765194
1102	5	0.028924822112337267
1102	6	-0.00951569329010482
1102	7	0.016555424896715465
1102	8	0.011559961873750524
1102	9	0.0051931532403273195
1102	10-11	0.04108926504394361
1102	12-13	-0.006910839111704092
1102	14-15	0.018782979109730036
1102	16-17	-0.011765760879214326
1102	18-19	0.02767129513598121
1102	20-21	0.054094469365693953
1102	22-23	-0.0015901639360365039
1102	24-25	0.04400137208967436
1102	26-27	0.04698953762962077
1102	28-29	0.042932173817426644
1102	30-31	0.05990505139858726
1102	32-33	0.10619657376447833
1102	34-35	0.09226691763926453
1102	36-37	0.10187468448870618
1102	38-39	0.12834431861556084
1102	40-41	0.09567642123756315
1102	42-43	0.040028529667424095
1102	44-45	0.03645126334821214
1102	46-47	0.08634423251960044
1102	48-49	0.08898459183684082
1102	50-51	0.058270496278296946
1102	52-53	0.08895446678553753
1102	54-55	0.1101522396318515
1102	56-57	0.08910651889065946
1102	58-59	0.07157408067641313
1102	60-61	0.07579271125235465
1102	62-63	0.048303087949477685
1102	64-65	0.027696082970372515
1102	66-67	0.07089952161676649
1102	68-69	0.06499238419949194
1102	70-71	0.11735660813464222
1102	72-73	0.03231276765447433
1102	74-75	0.08821319241754821
1102	76-77	0.01262092767231593
1102	78-79	0.009092008975152055
1102	80-81	-0.012321824782162594
1102	82-83	-0.04466580221230032
1102	84-85	0.017263187509847455
1102	86-87	0.022826149356568237
1102	88-89	0.03578108909051991
1102	90-91	0.03336180157761248
1102	92-93	0.048377848121326394
1102	94-95	0.017770766261673288
1102	96-97	0.05162470821846199
1102	98-99	0.11095944126152446
1102	100	0.1272198352790319
1103	1	-0.09083285607329827
1103	2	-0.10436794793761095
1103	3	-0.09071362786921355
1103	4	-0.07596599546235439
1103	5	-0.09740479099212962
1103	6	-0.045753396479518926
1103	7	-0.0756149337421661
1103	8	-0.09306778780818803
1103	9	-0.09961123494811375
1103	10-11	-0.0561415834962915
1103	12-13	-0.03730420610491336
1103	14-15	-0.08409599422288494
1103	16-17	-0.06264299194829448
1103	18-19	-0.11565844753555155
1103	20-21	-0.14743235446306358
1103	22-23	-0.09016837902563424
1103	24-25	-0.12816848344304788
1103	26-27	-0.09873550727088087
1103	28-29	-0.10812303967188086
1103	30-31	-0.13471936262056516
1103	32-33	-0.10388229674150296
1103	34-35	-0.10306784093179999
1103	36-37	-0.15159953924604963
1103	38-39	-0.15459766014922138
1103	40-41	-0.15312787459988186
1103	42-43	-0.1063019655958044
1103	44-45	-0.18165969706078045
1103	46-47	-0.2196597192759313
1103	48-49	-0.24344359837313334
1103	50-51	-0.24838945771161036
1103	52-53	-0.24050722340306407
1103	54-55	-0.19104480844442406
1103	56-57	-0.16562602160249895
1103	58-59	-0.164722099989703
1103	60-61	-0.194748286386492
1103	62-63	-0.17240017865681523
1103	64-65	-0.1732186398457074
1103	66-67	-0.17567473892054863
1103	68-69	-0.16651870550089143
1103	70-71	-0.2370697957038459
1103	72-73	-0.23682890566283987
1103	74-75	-0.15191000656576392
1103	76-77	-0.16511769282436717
1103	78-79	-0.09896210043734044
1103	80-81	-0.1385066397976047
1103	82-83	-0.2026436875950708
1103	84-85	-0.17332917520074886
1103	86-87	-0.17117971975588375
1103	88-89	-0.20699103276317032
1103	90-91	-0.22619435540804744
1103	92-93	-0.1837742195071712
1103	94-95	-0.21386020743483058
1103	96-97	-0.21653649388824192
1103	98-99	-0.1530781166473716
1103	100	-0.13594886517707394
1104	1	-0.10119316158694858
1104	2	-0.09314596900547656
1104	3	-0.12616014543872467
1104	4	-0.043329375236346834
1104	5	-0.08674201238083867
1104	6	-0.1122013772291055
1104	7	-0.08943011903359377
1104	8	-0.16031415445070962
1104	9	-0.10857770150237656
1104	10-11	-0.1080444742915958
1104	12-13	-0.07641015286221631
1104	14-15	-0.0886817945658791
1104	16-17	-0.09548319851327847
1104	18-19	-0.08576902184920954
1104	20-21	-0.10596856902979113
1104	22-23	-0.1846130757029698
1104	24-25	-0.112476788623745
1104	26-27	-0.13672910697590623
1104	28-29	-0.0901571213802086
1104	30-31	-0.07229798189313641
1104	32-33	-0.042076077641468146
1104	34-35	-0.05360317084898014
1104	36-37	-0.08428221490505194
1104	38-39	-0.09897996358125738
1104	40-41	-0.18473046608647792
1104	42-43	-0.045792659233960364
1104	44-45	2.2622949842343587E-5
1104	46-47	-0.04071451595444131
1104	48-49	-0.003000255498513127
1104	50-51	-0.11161073772933605
1104	52-53	-0.042084011464844195
1104	54-55	-5.894615771921963E-4
1104	56-57	-0.01191751018701126
1104	58-59	0.02418717390288805
1104	60-61	0.013402287019125936
1104	62-63	-0.055828030533731976
1104	64-65	0.005507636790945014
1104	66-67	-0.03750513798279087
1104	68-69	0.0034404602869742007
1104	70-71	0.025191749626188198
1104	72-73	0.019822682062958563
1104	74-75	-0.04053376429011024
1104	76-77	-0.05036901653112125
1104	78-79	-0.05211178988133014
1104	80-81	-0.05197738961032172
1104	82-83	-0.04839396464996781
1104	84-85	-0.06441653743859632
1104	86-87	-0.09156129538138202
1104	88-89	-0.08276023416402722
1104	90-91	-0.07418470130876642
1104	92-93	-0.08620050501783183
1104	94-95	-0.06047167637269624
1104	96-97	-0.06186078398450334
1104	98-99	-0.11086915499617334
1104	100	-0.06866301262187235
1105	1	-0.22895686964459117
1105	2	-0.19390491296922363
1105	3	-0.17018902006178394
1105	4	-0.08496956711358195
1105	5	-0.1487585567298737
1105	6	-0.0799238201885899
1105	7	-0.11009523141805033
1105	8	-0.08662708509360328
1105	9	-0.08164121089648546
1105	10-11	-0.1065795701481278
1105	12-13	-0.1010748739397016
1105	14-15	-0.16431705269165064
1105	16-17	-0.1993842621567552
1105	18-19	-0.23808286371741616
1105	20-21	-0.1726302759490892
1105	22-23	-0.21578105174294393
1105	24-25	-0.18174273917130535
1105	26-27	-0.12940636492655244
1105	28-29	-0.11955781971263946
1105	30-31	-0.17903800493372302
1105	32-33	-0.20247492342274143
1105	34-35	-0.21227862413569198
1105	36-37	-0.18746842502868333
1105	38-39	-0.11216502441170917
1105	40-41	-0.1101181473053856
1105	42-43	-0.2171682468785079
1105	44-45	-0.17004608836412416
1105	46-47	-0.26489567876549813
1105	48-49	-0.2779978269325625
1105	50-51	-0.2259484096444382
1105	52-53	-0.27867247830773323
1105	54-55	-0.21577884183452056
1105	56-57	-0.1521519386325636
1105	58-59	-0.16207781479572247
1105	60-61	-0.21470347258157574
1105	62-63	-0.28600251786688347
1105	64-65	-0.27337714519907763
1105	66-67	-0.32169356596862997
1105	68-69	-0.30762292323215945
1105	70-71	-0.30605448780683986
1105	72-73	-0.24676445761994614
1105	74-75	-0.24430207772972068
1105	76-77	-0.2295010333764722
1105	78-79	-0.19306678410749356
1105	80-81	-0.1666122701111874
1105	82-83	-0.23583783399175928
1105	84-85	-0.1792368782861189
1105	86-87	-0.23171157914733698
1105	88-89	-0.1944596967326433
1105	90-91	-0.17498496509892192
1105	92-93	-0.24842529034628313
1105	94-95	-0.2054220378527667
1105	96-97	-0.2360821378518736
1105	98-99	-0.2508006968003471
1105	100	-0.2564375431903372
1106	1	-0.14277605007545446
1106	2	-0.11371019075587441
1106	3	-0.061007737248665705
1106	4	-0.03485868141238058
1106	5	-0.05575167729045205
1106	6	-0.07912099706259568
1106	7	-0.06488741058165459
1106	8	-0.060718700909923484
1106	9	-0.10935531481222682
1106	10-11	-0.10896374584006452
1106	12-13	-0.14890015641204712
1106	14-15	-0.11646288402824467
1106	16-17	-0.15802782253288683
1106	18-19	-0.15267039020751128
1106	20-21	-0.1780962221914848
1106	22-23	-0.16140892039464205
1106	24-25	-0.09673447266224855
1106	26-27	-0.16400250402753613
1106	28-29	-0.17008602434933806
1106	30-31	-0.22117610222561268
1106	32-33	-0.19471199252596705
1106	34-35	-0.15499597608397409
1106	36-37	-0.09987098405235173
1106	38-39	-0.09989504901156465
1106	40-41	-0.17621707385725216
1106	42-43	-0.18103994160604486
1106	44-45	-0.1730443951274836
1106	46-47	-0.20400711556730045
1106	48-49	-0.14328494502250066
1106	50-51	-0.20401427282239837
1106	52-53	-0.15838458814447876
1106	54-55	-0.09018427882801916
1106	56-57	-0.07439750054219019
1106	58-59	-0.05101560166089314
1106	60-61	-0.12141851363457334
1106	62-63	-0.15607735573148318
1106	64-65	-0.08106413336771823
1106	66-67	-0.04341777872008379
1106	68-69	-0.02846211367335627
1106	70-71	-0.07782342756382121
1106	72-73	-0.1321538354886016
1106	74-75	-0.11226289900273656
1106	76-77	-0.058273077400627926
1106	78-79	-0.005387343621030993
1106	80-81	0.0056572526942559875
1106	82-83	-0.03203344275824804
1106	84-85	-0.009462350315764922
1106	86-87	-0.0443711853367077
1106	88-89	0.011953703265227489
1106	90-91	-0.046658127830681906
1106	92-93	-0.02374077547455755
1106	94-95	-0.05201772702137575
1106	96-97	-0.074907574738873
1106	98-99	-0.05447343622293843
1106	100	0.05015543041008286
1107	1	-0.25723800846966327
1107	2	-0.2233686470258931
1107	3	-0.19095480028260425
1107	4	-0.2192005286441585
1107	5	-0.2739510714591802
1107	6	-0.23923897198918098
1107	7	-0.19612217443315672
1107	8	-0.1986410552502278
1107	9	-0.18796325757152488
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2216	24-25	0.09123397031122238
2216	26-27	0.1271829766543462
2216	28-29	0.14002680047610738
2216	30-31	0.08412875420284394
2216	32-33	0.16039059361133923
2216	34-35	0.22280379931885363
2216	36-37	0.20499852376511996
2216	38-39	0.19280076589912198
2216	40-41	0.1828147621275491
2216	42-43	0.20190955161976376
2216	44-45	0.15284346875721866
2216	46-47	0.17304874557074612
2216	48-49	0.22738001281187792
2216	50-51	0.27776512840848966
2216	52-53	0.22885805241524082
2216	54-55	0.2480712970164518
2216	56-57	0.20851063431012307
2216	58-59	0.19702821624576927
2216	60-61	0.24688702435125975
2216	62-63	0.20498714596014622
2216	64-65	0.17798773039029214
2216	66-67	0.2001568058313552
2216	68-69	0.22990081001367457
2216	70-71	0.2480693410816741
2216	72-73	0.2102896652435362
2216	74-75	0.22207791136592903
2216	76-77	0.25130423440216987
2216	78-79	0.22722149864083718
2216	80-81	0.16079547498414115
2216	82-83	0.17285719129449006
2216	84-85	0.1726443415150527
2216	86-87	0.15971435266957457
2216	88-89	0.19706021832028853
2216	90-91	0.11787713075750617
2216	92-93	0.18354389275414462
2216	94-95	0.18184765364425814
2216	96-97	0.22650512409552803
2216	98-99	0.2157819485402186
2216	100	0.24865748581139968
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	50.0
3	272.0
4	86.0
5	138.0
6	324.0
7	735.0
8	1516.0
9	2865.0
10	3842.0
11	5022.0
12	7004.0
13	10138.0
14	11484.0
15	15327.0
16	20714.0
17	32322.0
18	37976.0
19	19198.0
20	12308.0
21	11164.0
22	11046.0
23	11184.0
24	11660.0
25	13712.0
26	16743.0
27	21327.0
28	26523.0
29	34040.0
30	42352.0
31	52838.0
32	66258.0
33	85842.0
34	120804.0
35	205282.0
36	339325.0
37	690865.0
38	1126310.0
39	668012.0
40	6228.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.30246667665959	20.702486769390756	14.859269388750796	22.135777165198856
2	14.541439924246857	21.875002504887537	40.38800507550332	23.195552495362286
3	16.464241915790094	28.943675707159635	31.696354858274173	22.8957275187761
4	11.116810888855403	18.59656688137017	38.8896280788151	31.39699415095933
5	11.573375900302626	39.31207871610551	34.73894498137249	14.375600402219371
6	27.47844148127249	39.611941319363176	18.418333050125625	14.491284149238707
7	24.50176817792711	35.55704818485234	22.88454530201163	17.056638335208916
8	25.632889071281777	34.90027348246089	20.46544385059885	19.00139359565848
9	26.652531210307483	15.704917717430162	21.541911824034944	36.10063924822741
10-11	23.497396092161132	27.5443407084895	28.549487607792596	20.408775591556775
12-13	25.166614990159196	25.98070366704601	28.984055003218817	19.86862633957597
14-15	22.350819004626437	26.515575355158493	28.15616286687421	22.977442773340854
16-17	19.77074336145105	29.9016841774526	28.610604365246033	21.716968095850312
18-19	19.72487414277823	27.305416419249323	32.969949897570885	19.999759540401556
20-21	21.51181617361808	26.118857465301716	32.888269750531414	19.48105661054879
22-23	21.034173155619584	26.34772487329459	32.88172830465031	19.736373666435515
24-25	20.508288435331984	26.483477566673713	32.93234500955225	20.07588898844205
26-27	20.30367938262742	26.553443932383768	32.98744078342879	20.155435901560022
28-29	20.17752867347506	26.530017911570702	33.11166517759814	20.180788237356097
30-31	20.398327040855992	26.595938526713347	32.9417934936381	20.063940938792555
32-33	20.22238261978017	26.601805848508203	33.01870118856728	20.157110343144346
34-35	20.504157045977852	26.484502786949456	32.82606186488091	20.18527830219178
36-37	20.526275431208727	26.631275093225838	32.485713209359965	20.356736266205477
38-39	20.47150350019357	26.672888401789784	32.44387279766741	20.411735300349232
40-41	20.5070868157134	26.592801891509648	32.41744449822133	20.48266679455562
42-43	20.548574173642706	26.735446503858597	32.256362328644876	20.459616993853817
44-45	20.730172186577025	26.790600533633715	31.87672588345261	20.60250139633665
46-47	20.855277501100772	26.952011204350075	31.296261761147708	20.896449533401444
48-49	20.96309692767402	27.175772249462437	30.6255931337554	21.235537689108142
50-51	21.02958114770589	27.36930119324371	30.40060937960465	21.200508279445753
52-53	20.977703395201257	27.592262181482802	30.246723586437152	21.183310836878796
54-55	21.03569031593392	27.603085704636733	30.246047887040962	21.11517609238839
56-57	20.966931337799558	27.41957935832346	30.25253035328493	21.36095895059205
58-59	21.034023303837724	27.18357590018427	30.489388073207962	21.29301272277004
60-61	21.046275499523684	27.42456463967192	30.369159053919798	21.160000806884597
62-63	20.92630749845837	27.221601177301675	30.691694123858206	21.160397200381748
64-65	21.012336426624344	27.103588177496523	30.652089257242782	21.231986138636348
66-67	20.97508739057559	27.215509895136652	30.506073823678836	21.30332889060892
68-69	21.259299959348894	27.180949258788583	30.32301399036637	21.236736791496156
70-71	21.11659538359271	27.228344159383393	30.348448123662774	21.30661233336112
72-73	21.15279157268135	27.33830239304376	30.245355950815263	21.26355008345963
74-75	21.205454293334984	27.28876649728426	30.094396914833844	21.41138229454691
76-77	21.11823886096123	27.30183645688194	29.96395751314856	21.61596716900827
78-79	21.113162608002494	27.34139495465355	30.06447987874304	21.480962558600915
80-81	20.842143568221275	27.39842781057848	30.163986591877855	21.595442029322385
82-83	20.949434196778782	27.406939741125335	30.162394147767042	21.48123191432884
84-85	20.984670637596153	27.271153335170645	29.98620412155709	21.757971905676108
86-87	20.996545063924735	27.375432200987454	29.922428923460515	21.7055938116273
88-89	20.919079328276318	27.236440526035533	29.970030370400252	21.874449775287893
90-91	20.906550753167892	27.26434631268484	29.831868784551425	21.997234149595847
92-93	20.837637723220993	27.3875687572431	29.92276478426841	21.8520287352675
94-95	20.777559581062306	27.213783798225926	29.971184674575184	22.037471946136584
96-97	20.81609703039975	27.389390426006866	29.760940059479108	22.03357248411428
98-99	20.584040598243785	27.386621645378806	29.899097525877533	22.130240230499872
100	20.76710547092395	27.38577271754502	29.8088368058014	22.038285005729634
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4697.0
1	2728.5
2	2461.5
3	4283.5
4	2790.5
5	1672.0
6	4201.5
7	11375.0
8	20537.5
9	22690.5
10	14428.0
11	6539.0
12	5014.5
13	5197.5
14	5572.0
15	6008.5
16	6637.0
17	7368.5
18	8041.0
19	8764.0
20	10092.0
21	12109.0
22	14892.5
23	18629.5
24	23668.0
25	29057.0
26	35905.0
27	44690.0
28	54979.0
29	66503.0
30	74638.0
31	81165.5
32	87305.5
33	94443.5
34	101172.5
35	104704.0
36	106680.5
37	109576.5
38	112667.5
39	113447.5
40	114847.0
41	115491.5
42	114078.5
43	113216.0
44	112331.5
45	111728.5
46	113363.0
47	112971.5
48	109975.5
49	109476.5
50	110976.5
51	107985.5
52	101790.5
53	97260.5
54	94065.0
55	90206.5
56	81411.0
57	71451.5
58	65983.0
59	62417.5
60	55920.5
61	46832.5
62	39755.5
63	32687.5
64	25705.5
65	20756.0
66	16355.5
67	12595.0
68	9636.0
69	7231.5
70	5152.5
71	3790.5
72	2921.5
73	2122.0
74	1654.0
75	1297.0
76	829.5
77	599.0
78	466.0
79	261.5
80	115.0
81	55.0
82	33.0
83	19.0
84	9.0
85	6.0
86	4.5
87	2.5
88	2.5
89	2.0
90	2.0
91	1.5
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	1.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005049646845333325
2	0.00408780935098412
3	0.0012290145761128727
4	0.0
5	0.04258802683312868
6	0.0
7	0.0
8	0.0
9	5.343541635273359E-5
10-11	0.0
12-13	1.3358854088183398E-5
14-15	0.0
16-17	0.0
18-19	9.35119786172838E-5
20-21	0.0
22-23	1.3358854088183398E-5
24-25	1.3358854088183398E-5
26-27	1.870239572345676E-4
28-29	1.0687083270546719E-4
30-31	1.870239572345676E-4
32-33	0.001402679679259257
34-35	9.084020779964711E-4
36-37	0.0011488614515837725
38-39	0.0011488614515837725
40-41	1.469473949700174E-4
42-43	6.412249962328031E-4
44-45	1.736651031463842E-4
46-47	1.0687083270546719E-4
48-49	1.0687083270546719E-4
50-51	3.473302062927684E-4
52-53	6.412249962328031E-4
54-55	7.480958289382704E-4
56-57	0.00208398123775661
58-59	5.076364553509692E-4
60-61	0.0012156557220246893
62-63	0.001068708327054672
64-65	0.0015629859283174574
66-67	3.3397135220458496E-4
68-69	2.8053593585185136E-4
70-71	3.2061249811640155E-4
72-73	2.271005194991178E-4
74-75	6.145072880564364E-4
76-77	8.282489534673708E-4
78-79	0.0010820671811428552
80-81	8.015312452910038E-4
82-83	0.0015897036364938245
84-85	0.00137596197108289
86-87	0.00200382811322751
88-89	0.0012557322842892396
90-91	0.001202296867936506
92-93	0.0012958088465537898
94-95	9.618374943492048E-4
96-97	2.404593735873012E-4
98-99	3.740479144691352E-4
100	3.473302062927684E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3742836.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.805764976876105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.526905970578	32.28175396445064
2	17.088729485839384	16.33879571106901
3	6.490694533522253	9.30877852218778
4	3.081018936802297	5.891618687283012
5	1.644577801990029	3.9310149944061417
6	1.044232363691337	2.995219617592553
7	0.6536209250799339	2.1872793829837764
8	0.4655634574079152	1.7805293781331732
9	0.33382670225720235	1.4362956784002072
>10	1.5339565526510788	12.647107762687737
>50	0.08659163054241888	2.842428352404513
>100	0.04619132669619587	3.880978207690919
>500	0.0025774574892770226	0.8413868346917344
>1k	0.0011206336687242528	0.7814924875938057
>5k	2.2412673374485023E-4	0.6190331238489402
>10k+	1.6809505030863768E-4	2.2362872945760546
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35858	0.9580435797881606	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28425	0.7594508549132262	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19203	0.5130601501107717	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6508	0.1738788448117951	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5887	0.15728714803427132	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5456	0.14577181581025725	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5259	0.140508427299513	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4447	0.11881364826030316	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	2.6717708176366797E-5	0.0
12-13	0.0	0.0	0.0	4.007656226455019E-5	0.0
14-15	0.0	0.0	0.0	5.343541635273359E-5	0.0
16-17	0.0	0.0	0.0	1.2022968679365059E-4	0.0
18-19	0.0	0.0	0.0	1.469473949700174E-4	0.0
20-21	0.0	0.0	0.0	2.1374166541093437E-4	0.0
22-23	0.0	0.0	0.0	4.542010389982355E-4	0.0
24-25	0.0	0.0	0.0	0.0010553494729664886	0.0
26-27	0.0	0.0	0.0	0.0013492442629065233	0.0
28-29	0.0	0.0	0.0	0.00267177081763668	0.0
30-31	0.0	0.0	0.0	0.005664154133389761	0.0
32-33	0.0	0.0	0.0	0.01127487285042679	0.0
34-35	0.0	0.0	0.0	0.019356979573777744	0.0
36-37	0.0	0.0	0.0	0.03343721178272305	0.0
38-39	0.0	0.0	0.0	0.055586191860931125	0.0
40-41	0.0	0.0	0.0	0.07968556463601398	0.0
42-43	0.0	0.0	0.0	0.10196813325510388	0.0
44-45	0.0	0.0	0.0	0.1281782049761197	0.0
46-47	0.0	0.0	0.0	0.16197610581922373	0.0
48-49	0.0	0.0	0.0	0.20016906965734005	0.0
50-51	0.0	0.0	0.0	0.24283724961499784	1.3358854088183398E-5
52-53	0.0	0.0	0.0	0.28578596550850743	2.6717708176366797E-5
54-55	0.0	0.0	0.0	0.33052476784983364	2.6717708176366797E-5
56-57	0.0	0.0	0.0	0.3860174477321475	2.6717708176366797E-5
58-59	0.0	0.0	0.0	0.45380027337559004	2.6717708176366797E-5
60-61	0.0	0.0	0.0	0.5286232151235053	2.6717708176366797E-5
62-63	0.0	0.0	0.0	0.605156090194708	2.6717708176366797E-5
64-65	0.0	0.0	0.0	0.6828244678634062	2.6717708176366797E-5
66-67	0.0	0.0	0.0	0.7763898818970427	2.6717708176366797E-5
68-69	0.0	0.0	0.0	0.8859324854201467	2.6717708176366797E-5
70-71	0.0	0.0	0.0	1.011385484162277	2.6717708176366797E-5
72-73	0.0	0.0	0.0	1.1445732594214655	2.6717708176366797E-5
74-75	0.0	0.0	0.0	1.2764652258341003	2.6717708176366797E-5
76-77	0.0	0.0	0.0	1.4197122182216906	2.6717708176366797E-5
78-79	0.0	0.0	0.0	1.5924555604359902	2.6717708176366797E-5
80-81	0.0	0.0	0.0	1.7839280160819229	2.6717708176366797E-5
82-83	0.0	0.0	0.0	1.9915780440286457	2.6717708176366797E-5
84-85	0.0	0.0	0.0	2.196195612097351	2.6717708176366797E-5
86-87	0.0	0.0	0.0	2.415254101435382	2.6717708176366797E-5
88	0.0	0.0	0.0	2.59749558890638	2.6717708176366797E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11870	0.0	65.4119	1
GTATCAA	23840	0.0	46.04209	1
TATCAAC	31250	0.0	35.018776	2
ATCAACG	31295	0.0	34.844944	3
TCAACGC	31535	0.0	34.579754	4
CAACGCA	32760	0.0	33.31542	5
AACGCAG	33485	0.0	32.702553	6
GTGGTAT	3590	0.0	31.832613	1
TGGTATC	3655	0.0	30.23675	2
ACGCAGA	37380	0.0	29.23208	7
CGCAGAG	37775	0.0	28.801996	8
TACCTGG	2970	0.0	27.55164	2
GCAGAGT	40275	0.0	26.722431	9
GTACATG	23410	0.0	25.854586	1
TACATGG	23625	0.0	25.14121	2
CATGGGT	10505	0.0	25.067207	4
TAACGCA	385	0.0	24.42774	4
TATAACG	350	0.0	24.185724	2
ACATGGG	24020	0.0	24.11858	3
GAGTACT	26290	0.0	23.811663	12-13
>>END_MODULE