FastQCFastQC Report
Wed 25 May 2016
SRR2431391_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431391_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4619639
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160880.3482523201488255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60500.047.6709671
GTATCAA108050.038.1877171
TCAACGC125450.031.7229274
ATCAACG126250.031.450463
CAACGCA134950.029.5236435
TATCAAC143350.028.4542242
AACGCAG143800.027.8445426
GTACATG240200.027.2068421
CATGGGT106900.026.7106554
TACATGG239450.026.5636162
TACCTGG36750.025.9874742
ACATGGG245000.025.1740193
CGCAGAG161150.024.1462678
GTACCTG41400.024.0926421
ACGCAGA164750.023.761527
GTGGTAT32000.021.0249481
GCAGAGT178650.020.939659
ATGGGTA48750.020.6489775
ATGGGTG59250.020.5622585
ACCTGGG45750.020.1549113
Produced by FastQC (version 0.11.5)