##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431391_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4619639
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.272456787207833	31.0	31.0	34.0	26.0	34.0
2	30.439119593543996	33.0	31.0	34.0	26.0	34.0
3	30.478170263953526	33.0	31.0	34.0	26.0	34.0
4	33.70305082280239	37.0	35.0	37.0	28.0	37.0
5	33.81329168794358	37.0	35.0	37.0	28.0	37.0
6	33.782220428912304	37.0	35.0	37.0	29.0	37.0
7	33.73300857491245	37.0	35.0	37.0	28.0	37.0
8	33.69062366128609	37.0	35.0	37.0	28.0	37.0
9	35.06028241600696	39.0	37.0	39.0	27.0	39.0
10-11	35.09036690962216	39.0	37.0	39.0	27.0	39.0
12-13	35.023977739386126	39.0	37.0	39.0	27.0	39.0
14-15	36.27544316774536	40.0	37.0	41.0	27.0	41.0
16-17	36.27010595416655	40.0	37.0	41.0	27.0	41.0
18-19	36.272264347928484	40.0	37.0	41.0	27.0	41.0
20-21	36.36526241552641	40.0	37.0	41.0	27.0	41.0
22-23	36.31679466728894	40.0	37.0	41.0	27.0	41.0
24-25	36.36220936311257	40.0	37.0	41.0	27.0	41.0
26-27	36.409454937929134	40.0	37.0	41.0	28.0	41.0
28-29	36.37687003248522	40.0	37.0	41.0	28.5	41.0
30-31	36.301937229294325	40.0	37.0	41.0	28.5	41.0
32-33	36.260457797676395	40.0	37.0	41.0	28.5	41.0
34-35	36.183015058103024	40.0	36.5	41.0	28.0	41.0
36-37	36.03196191304126	40.0	36.0	41.0	27.0	41.0
38-39	35.898989185085675	40.0	36.0	41.0	27.0	41.0
40-41	35.83376753032001	40.0	36.0	41.0	27.0	41.0
42-43	35.63901302677547	40.0	35.5	41.0	26.5	41.0
44-45	35.44779386441235	40.0	35.0	41.0	26.0	41.0
46-47	35.30111941647388	40.0	35.0	41.0	25.5	41.0
48-49	35.14926555949502	39.5	35.0	41.0	25.0	41.0
50-51	34.997537361685616	39.0	35.0	41.0	24.5	41.0
52-53	34.82697955403009	39.0	34.0	41.0	24.0	41.0
54-55	34.65295091239813	39.0	34.0	41.0	24.0	41.0
56-57	34.45341703107104	39.0	34.0	40.5	23.5	41.0
58-59	34.22622644323506	38.0	33.5	40.0	23.0	41.0
60-61	33.982077820366484	38.0	33.0	40.0	22.0	41.0
62-63	33.892380118879416	38.0	33.0	40.0	22.0	41.0
64-65	33.64748230327088	37.5	33.0	40.0	21.0	41.0
66-67	33.391307524245946	37.0	33.0	40.0	20.0	41.0
68-69	33.1020098107233	36.5	32.5	40.0	19.0	41.0
70-71	32.77616324565621	36.0	32.0	39.0	18.0	41.0
72-73	32.437897095422386	36.0	32.0	39.0	15.5	41.0
74-75	32.15981714155586	35.0	32.0	39.0	13.0	40.0
76-77	31.902889165149052	35.0	32.0	38.0	10.0	40.0
78-79	31.554646586021114	35.0	31.5	37.0	7.5	39.0
80-81	31.223542034345108	35.0	31.0	37.0	4.5	39.0
82-83	30.888326338919555	35.0	31.0	36.0	2.0	39.0
84-85	30.579565416258717	35.0	31.0	36.0	2.0	37.5
86-87	30.28938852148404	35.0	31.0	36.0	2.0	37.0
88-89	30.01312093867075	35.0	31.0	35.0	2.0	37.0
90-91	29.772952388703967	34.5	30.0	35.0	2.0	36.0
92-93	29.53072025757857	34.0	30.0	35.0	2.0	36.0
94-95	29.297307105598513	34.0	30.0	35.0	2.0	36.0
96-97	29.079657631256467	34.0	30.0	35.0	2.0	35.5
98-99	28.840553883106452	34.0	30.0	35.0	2.0	35.0
100	28.64931675397147	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2878722719639164
1101	2	0.3279238506498139
1101	3	0.33299111059290354
1101	4	0.37568823497196746
1101	5	0.2862061374069782
1101	6	0.3363665688358779
1101	7	0.3220953668505899
1101	8	0.3365806346090281
1101	9	0.3184849466482902
1101	10-11	0.32001149832824893
1101	12-13	0.2302624957364614
1101	14-15	0.37817402268454714
1101	16-17	0.3203232569277361
1101	18-19	0.30579740813966794
1101	20-21	0.3349129262221737
1101	22-23	0.40668487009284604
1101	24-25	0.39224980499739814
1101	26-27	0.35567750100127427
1101	28-29	0.3879178380120507
1101	30-31	0.40269650763293185
1101	32-33	0.3967375412854608
1101	34-35	0.4030049662455468
1101	36-37	0.3585198716714473
1101	38-39	0.4374577570206526
1101	40-41	0.3873580275721693
1101	42-43	0.3521632513492179
1101	44-45	0.33196239235145697
1101	46-47	0.36511429733167944
1101	48-49	0.3916581322679562
1101	50-51	0.3647676321862292
1101	52-53	0.23819404502891928
1101	54-55	0.309150481046359
1101	56-57	0.23235679513101104
1101	58-59	0.2700607484382189
1101	60-61	0.31979501441085034
1101	62-63	0.3259091856263936
1101	64-65	0.35825840047949953
1101	66-67	0.354589262497754
1101	68-69	0.2711035427934618
1101	70-71	0.26124895824560923
1101	72-73	0.2490493374951228
1101	74-75	0.3530450877887432
1101	76-77	0.3546143295878217
1101	78-79	0.3082492640068075
1101	80-81	0.20284177444224838
1101	82-83	0.21870045235773716
1101	84-85	0.2342299157959573
1101	86-87	0.20574332014862762
1101	88-89	0.23992381471059687
1101	90-91	0.15035556784281923
1101	92-93	0.17650607043347577
1101	94-95	0.16852282294827958
1101	96-97	0.16162668872717845
1101	98-99	0.11977690919421491
1101	100	0.14595242569963318
1102	1	0.02951936535751898
1102	2	0.07858458067725849
1102	3	0.06093212341082932
1102	4	0.168311907208178
1102	5	0.15494216178009879
1102	6	0.09437428432571693
1102	7	0.08126536428402176
1102	8	0.09245353215495555
1102	9	0.11722352350582099
1102	10-11	0.15360219308576717
1102	12-13	0.09590225933020946
1102	14-15	0.1293557792952953
1102	16-17	0.1139173873968744
1102	18-19	0.16397921940626503
1102	20-21	0.09336204402327297
1102	22-23	0.10336826736507021
1102	24-25	0.08865502795521962
1102	26-27	0.08852870073938135
1102	28-29	0.05543191682296822
1102	30-31	0.10665198509261131
1102	32-33	0.04372028496953817
1102	34-35	0.1355007973285467
1102	36-37	0.11435054475819584
1102	38-39	0.1354139452880787
1102	40-41	0.09265119984542736
1102	42-43	0.10505779261058734
1102	44-45	0.1033320890086884
1102	46-47	-0.02333269621639289
1102	48-49	0.0257866800029376
1102	50-51	-0.001778847963137764
1102	52-53	-0.012829519529631739
1102	54-55	-0.10515932829771657
1102	56-57	-0.06732871540672392
1102	58-59	-0.07453831007255474
1102	60-61	-0.029474442577679838
1102	62-63	-0.007945772968987797
1102	64-65	0.009514794720580255
1102	66-67	0.0265609623199623
1102	68-69	0.07587342054748802
1102	70-71	-0.0019068661378369711
1102	72-73	-0.009468769226330664
1102	74-75	0.017636170490078484
1102	76-77	0.008808229093748565
1102	78-79	-0.031014017434458196
1102	80-81	-0.005901231229280768
1102	82-83	-0.06203927086392724
1102	84-85	-0.03697574288729655
1102	86-87	-0.11737313793836535
1102	88-89	-0.06821669476641645
1102	90-91	-0.06366891121512097
1102	92-93	-0.06284368134727458
1102	94-95	-0.08284043896901849
1102	96-97	-0.10675767879902409
1102	98-99	-0.17437850019921086
1102	100	-0.18221226758105047
1103	1	-0.28941614605061616
1103	2	-0.18718887564879338
1103	3	-0.19437097175051576
1103	4	-0.13562064169742882
1103	5	-0.10837850120015702
1103	6	-0.18179330274924155
1103	7	-0.10792187312479484
1103	8	-0.061245888860931075
1103	9	-0.011274943306212037
1103	10-11	-0.06539074691789182
1103	12-13	-0.10943026127632294
1103	14-15	-0.1462649239404996
1103	16-17	-0.12906371369974323
1103	18-19	-0.16325038146752036
1103	20-21	-0.23532765721313353
1103	22-23	-0.22753882594967934
1103	24-25	-0.1343435990255628
1103	26-27	-0.23815844376146345
1103	28-29	-0.2061051769478297
1103	30-31	-0.1953899439759894
1103	32-33	-0.19281764841402804
1103	34-35	-0.20917171285944391
1103	36-37	-0.25459878355617604
1103	38-39	-0.1942092975239973
1103	40-41	-0.23956011490466267
1103	42-43	-0.3072967398898143
1103	44-45	-0.47326444154619907
1103	46-47	-0.5930013317979288
1103	48-49	-0.5098992482981757
1103	50-51	-0.5916007592135841
1103	52-53	-0.644511850709435
1103	54-55	-0.5799445296593362
1103	56-57	-0.5638518583002821
1103	58-59	-0.6037336187752302
1103	60-61	-0.4797530793144915
1103	62-63	-0.4615152788206345
1103	64-65	-0.5137975354470967
1103	66-67	-0.49450780095612856
1103	68-69	-0.5637467496488924
1103	70-71	-0.537017235300425
1103	72-73	-0.503504501952726
1103	74-75	-0.516065725318839
1103	76-77	-0.48573487290929407
1103	78-79	-0.5215343534520755
1103	80-81	-0.5637952612705348
1103	82-83	-0.5576542784970613
1103	84-85	-0.5642220832538314
1103	86-87	-0.6353003730586622
1103	88-89	-0.6599073638056545
1103	90-91	-0.6503636319594541
1103	92-93	-0.6453730625177982
1103	94-95	-0.7371152126612621
1103	96-97	-0.762531516850661
1103	98-99	-0.8159367650582183
1103	100	-0.8041637276859994
1104	1	-0.21409270194343577
1104	2	-0.1626273407092924
1104	3	-0.11796100310453994
1104	4	-0.06024224744547979
1104	5	-0.09499476044886279
1104	6	-0.1597385562058733
1104	7	-0.17488942397528717
1104	8	-0.18064631426398847
1104	9	-0.1735121148012766
1104	10-11	-0.23073227261750873
1104	12-13	-0.09893183419585938
1104	14-15	-0.2402790063784721
1104	16-17	-0.17643938258787273
1104	18-19	-0.17727204983876277
1104	20-21	-0.2541622957787908
1104	22-23	-0.12721616287417703
1104	24-25	-0.14738823476498197
1104	26-27	-0.12640693330745023
1104	28-29	-0.0959368409682142
1104	30-31	-0.1527581945945684
1104	32-33	-0.1963896347031735
1104	34-35	-0.1950248865717512
1104	36-37	-0.3092801672117105
1104	38-39	-0.2539030101361419
1104	40-41	-0.23482956992741322
1104	42-43	-0.30453813825801745
1104	44-45	-0.3311995544096362
1104	46-47	-0.39324314873558563
1104	48-49	-0.47711967191024485
1104	50-51	-0.5889466808340345
1104	52-53	-0.6842596023194432
1104	54-55	-0.48550650072874646
1104	56-57	-0.4568734440194362
1104	58-59	-0.6161716589136503
1104	60-61	-0.5968269491661857
1104	62-63	-0.53674109680356
1104	64-65	-0.5044489435490931
1104	66-67	-0.398817013089257
1104	68-69	-0.46307173181562433
1104	70-71	-0.5097520261859714
1104	72-73	-0.5458039640425341
1104	74-75	-0.4842656885780201
1104	76-77	-0.47064194897011546
1104	78-79	-0.42899703913881027
1104	80-81	-0.43283997977548694
1104	82-83	-0.48249442564456757
1104	84-85	-0.45120869331927693
1104	86-87	-0.3789899263396208
1104	88-89	-0.4416730453620339
1104	90-91	-0.48763607239498086
1104	92-93	-0.5983572354013909
1104	94-95	-0.5292248959635231
1104	96-97	-0.5314372423449214
1104	98-99	-0.575608041585248
1104	100	-0.4295441559100759
1105	1	-0.1587296697836571
1105	2	-0.1643955287198402
1105	3	-0.17549574999406659
1105	4	-0.06378292578990141
1105	5	-0.06139265674205063
1105	6	-0.06596727935009739
1105	7	-0.11129852169047894
1105	8	-0.05960535175445614
1105	9	-0.16275309646841407
1105	10-11	-0.24171633812713367
1105	12-13	-0.23245183040268103
1105	14-15	-0.2782506878621831
1105	16-17	-0.2652150267476614
1105	18-19	-0.20725489649797169
1105	20-21	-0.16817095700210416
1105	22-23	-0.17103263976045469
1105	24-25	-0.22366468226662306
1105	26-27	-0.16462624359241573
1105	28-29	-0.15451082185872167
1105	30-31	-0.14585364556086944
1105	32-33	-0.11368396687369398
1105	34-35	-0.1431602228617379
1105	36-37	-0.1751269492622356
1105	38-39	-0.18947168354238642
1105	40-41	-0.3024689027302401
1105	42-43	-0.2969565640343461
1105	44-45	-0.35943064213751086
1105	46-47	-0.33829712985258453
1105	48-49	-0.4671358854274601
1105	50-51	-0.33403987551039904
1105	52-53	-0.30621892137801865
1105	54-55	-0.34565148947059043
1105	56-57	-0.39203860185239137
1105	58-59	-0.36623361348410555
1105	60-61	-0.4437168921465755
1105	62-63	-0.367999116428976
1105	64-65	-0.3650513940945004
1105	66-67	-0.4236678679241237
1105	68-69	-0.283869956351559
1105	70-71	-0.31247162061375633
1105	72-73	-0.2743840504425492
1105	74-75	-0.3227570840316858
1105	76-77	-0.3811803851799169
1105	78-79	-0.36803821132530246
1105	80-81	-0.3546194951568822
1105	82-83	-0.2830441778563788
1105	84-85	-0.3880956052794531
1105	86-87	-0.34215890668654225
1105	88-89	-0.3948422002539864
1105	90-91	-0.4049757015654727
1105	92-93	-0.419015511824238
1105	94-95	-0.4383886172663267
1105	96-97	-0.449695985986704
1105	98-99	-0.5397600024983973
1105	100	-0.5230315908856404
1106	1	-0.1411004510159195
1106	2	-0.14869561016300992
1106	3	-0.052855937437964684
1106	4	-0.1083306313537804
1106	5	-0.07195521799098259
1106	6	-0.05667449125257917
1106	7	-0.13305092914240646
1106	8	-0.20871593286840806
1106	9	-0.21097927425492458
1106	10-11	-0.18438584710923323
1106	12-13	-0.1038249741627979
1106	14-15	-0.13637557641581566
1106	16-17	-0.11130039352017462
1106	18-19	-0.21791463756902374
1106	20-21	-0.26568256183854544
1106	22-23	-0.16885097434924745
1106	24-25	-0.1910425497435284
1106	26-27	-0.23494368407284583
1106	28-29	-0.19035279183279386
1106	30-31	-0.11386364618519451
1106	32-33	-0.21352530677698667
1106	34-35	-0.13864996694601928
1106	36-37	-0.22973846763238015
1106	38-39	-0.18620660348533136
1106	40-41	-0.18778999641615002
1106	42-43	-0.17245716159307278
1106	44-45	-0.1956326266401831
1106	46-47	-0.2526482011165072
1106	48-49	-0.17488210416598093
1106	50-51	-0.17243475509260975
1106	52-53	-0.2766011894380256
1106	54-55	-0.3022438867821222
1106	56-57	-0.29368343296887645
1106	58-59	-0.43143150215961157
1106	60-61	-0.38655326968103765
1106	62-63	-0.3076148142873265
1106	64-65	-0.3099144136283556
1106	66-67	-0.22096924473112978
1106	68-69	-0.22305997419572066
1106	70-71	-0.24820649626428803
1106	72-73	-0.2640240875272326
1106	74-75	-0.24862072489013087
1106	76-77	-0.1926048407499401
1106	78-79	-0.2532425920448347
1106	80-81	-0.24065056302772447
1106	82-83	-0.2526093255786712
1106	84-85	-0.1653997394019484
1106	86-87	-0.2989557271528014
1106	88-89	-0.3268335795831341
1106	90-91	-0.3239802826006972
1106	92-93	-0.3033882924125635
1106	94-95	-0.31390278760687806
1106	96-97	-0.3003824502131174
1106	98-99	-0.303565027900234
1106	100	-0.30567885519050364
1107	1	-0.404586421476985
1107	2	-0.5061989060297023
1107	3	-0.4685323295539341
1107	4	-0.4236069505949871
1107	5	-0.3734999320682775
1107	6	-0.394520321659634
1107	7	-0.4511931215601095
1107	8	-0.3912910764518287
1107	9	-0.375632345330807
1107	10-11	-0.4137922309799933
1107	12-13	-0.44600837191364207
1107	14-15	-0.4952166240429676
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2216	90-91	0.3192476261010633
2216	92-93	0.25547114049398445
2216	94-95	0.2301535361726934
2216	96-97	0.24570860384041637
2216	98-99	0.2808300702766857
2216	100	0.25277721191258706
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	142491.0
3	25248.0
4	17046.0
5	14709.0
6	11155.0
7	10059.0
8	11071.0
9	11726.0
10	12643.0
11	13526.0
12	14950.0
13	16185.0
14	17137.0
15	17305.0
16	17143.0
17	16915.0
18	16498.0
19	16573.0
20	16098.0
21	16860.0
22	19971.0
23	27964.0
24	41998.0
25	55675.0
26	59361.0
27	49197.0
28	48296.0
29	58992.0
30	69744.0
31	81878.0
32	103245.0
33	136694.0
34	191037.0
35	290343.0
36	475628.0
37	815625.0
38	1151498.0
39	505829.0
40	1326.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.99214925734958	21.71287826665247	14.132551049017161	22.162421426980785
2	14.680401954481443	22.2173411983829	40.75971955554736	22.342537291588293
3	17.028596658283714	29.116571896733774	30.856910882578028	22.997920562404488
4	11.694996878700376	18.9795616129801	39.699635353277124	29.6258061550424
5	11.74012658186672	40.55869944017288	35.207086155691144	12.494087822269256
6	27.18712585195007	40.916218781146284	19.022832251050133	12.873823115853511
7	24.731184659139526	35.552728359003034	23.2914301212255	16.42465686063194
8	24.382469964189006	37.16168490356505	21.140159753453684	17.31568537879226
9	25.22680164521348	18.335575034166986	21.59299376438966	34.844629556229876
10-11	22.310615824850913	29.375824504322622	29.40111471901326	18.912444951813207
12-13	23.026191591505402	27.61699028206121	28.883934397820845	20.472883728612548
14-15	20.94548528065429	29.04313720138618	26.42312388194631	23.588253636013217
16-17	20.13098830605439	31.998941679294603	27.16807371769046	20.701996296960544
18-19	20.30303795008849	30.852256462915328	28.922958819577516	19.92174676741866
20-21	22.074306430140034	29.711286396252728	28.233038259902898	19.98136891370434
22-23	21.611211384240313	29.745237658146788	28.300351506506743	20.343199451106152
24-25	21.17588637427002	29.841386226974198	28.284750983416224	20.69797641533956
26-27	20.780348883694135	29.90403039418405	28.34537317367534	20.970247548446473
28-29	20.846589613718987	29.84484533542293	28.556711247481193	20.751853803376893
30-31	21.063079735366806	30.10089408639192	28.258023539101263	20.57800263914001
32-33	20.762572668711908	29.92982104610452	28.608502814685643	20.699103470497924
34-35	21.097267418655896	29.739836420951505	28.438720676021312	20.724175484371283
36-37	21.162130371594152	29.7410612126055	28.21535781525591	20.88145060054444
38-39	20.96385080198143	29.878857115128472	28.422235318724294	20.735056764165805
40-41	20.877291621063414	29.621938778328488	28.592349527673942	20.90842007293416
42-43	20.851717863177505	29.66824444348165	28.528984879337983	20.95105281400286
44-45	21.026273146305112	29.713681753477054	28.468762352486465	20.79128274773137
46-47	21.05540956542093	29.837292938890265	28.308723696728	20.798573798960806
48-49	20.97701326846255	29.643309263508417	28.381346790164656	20.99833067786437
50-51	20.93305660541673	29.611035235149945	28.311308724392404	21.144599435040917
52-53	20.964563389679235	29.672788677961844	28.355884691289024	21.0067632410699
54-55	21.05610685460155	29.876299455792125	28.259847614863475	20.80774607474285
56-57	21.030951797364825	29.6583527850054	28.108082230668764	21.202613186961013
58-59	21.02194048914739	29.3915315145129	28.414187769928805	21.17234022641091
60-61	21.1670057823879	29.699247590942495	28.079399112576493	21.054347514093116
62-63	20.955374746508962	29.627086034674498	28.279776559644098	21.137762659172445
64-65	21.04360484164888	29.475917665324893	28.34467965044209	21.135797842584132
66-67	20.839969066939318	29.56298387061836	28.22702440419199	21.37002265825033
68-69	21.203401155054905	29.734340404252972	28.015686374747222	21.0465720659449
70-71	21.127270352472618	29.451151498925682	28.151780406447823	21.269797742153873
72-73	20.97171832255018	29.55696298494402	28.347610992100535	21.123707700405262
74-75	20.953786868751415	29.650485373802272	28.251560420184358	21.14416733726196
76-77	20.98066919933822	29.59692613462512	28.149490803044618	21.272913862992038
78-79	21.063085530521967	29.550871642319326	28.332726630956245	21.05331619620246
80-81	20.992018605886457	29.566631323541024	28.26739506216567	21.173955008406857
82-83	20.893092538173498	29.49665247357344	28.520556877620585	21.089698110632472
84-85	20.870526611212853	29.452840842684523	28.300888630899234	21.375743915203387
86-87	21.139347271118513	29.4417199238032	28.19328086526496	21.225651939813325
88-89	20.953666095985742	29.370372063309404	28.362372493107312	21.31358934759754
90-91	20.909626081724454	29.367166484427337	28.253638152304422	21.469569281543787
92-93	21.00346188653971	29.4164180326555	28.26643789593892	21.31368218486587
94-95	20.916258653459483	29.308079565851635	28.43520369035206	21.340458090336817
96-97	20.899888878539695	29.43225165003455	28.221460954778117	21.44639851664764
98-99	20.81738403315288	29.432865631613954	28.22078380655909	21.52896652867408
100	20.814116829411173	29.56315058806402	28.114828468329815	21.507904114194996
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14549.0
1	10663.0
2	5850.0
3	4273.5
4	3135.5
5	2357.5
6	1983.0
7	2023.0
8	2320.0
9	2407.0
10	2029.0
11	1663.5
12	1585.5
13	1652.0
14	1814.0
15	1994.5
16	2272.0
17	2804.0
18	3641.5
19	4739.0
20	6446.0
21	8985.5
22	13432.5
23	20793.5
24	28434.5
25	36084.0
26	47288.5
27	61524.5
28	79540.0
29	95831.5
30	104482.5
31	114512.5
32	126332.5
33	136755.0
34	147010.0
35	155224.5
36	158681.0
37	160086.5
38	161228.0
39	160828.0
40	160711.0
41	158165.5
42	153525.0
43	149630.5
44	147386.0
45	144403.0
46	140990.0
47	136946.5
48	131332.0
49	127657.0
50	126450.5
51	122388.0
52	113576.0
53	106061.5
54	101554.0
55	98148.5
56	90592.5
57	80668.0
58	73050.5
59	65872.0
60	58040.0
61	48923.5
62	40850.0
63	33304.5
64	26074.0
65	21033.5
66	16873.0
67	12972.0
68	9767.5
69	7441.5
70	5699.0
71	4390.5
72	3432.0
73	2599.0
74	2064.5
75	1635.5
76	1174.0
77	863.5
78	675.5
79	503.0
80	367.5
81	290.0
82	232.0
83	184.0
84	148.5
85	120.5
86	109.0
87	96.5
88	82.0
89	68.0
90	59.0
91	54.0
92	50.5
93	42.5
94	33.5
95	39.0
96	39.0
97	35.0
98	42.0
99	41.5
100	59.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16984011088312312
2	0.16611687623210386
3	0.17958113177241772
4	0.16836813439318526
5	0.1817025096549752
6	0.07182379402373215
7	0.07286283625192358
8	0.06277546795323184
9	0.05647627444482134
10-11	0.06989723655896056
12-13	0.06876078412187618
14-15	0.0666394062393187
16-17	0.06258064753544595
18-19	0.06685587337019192
20-21	0.0684252600690227
22-23	0.06670434637858066
24-25	0.06665022959586236
26-27	0.06990805991550422
28-29	0.06746198133663692
30-31	0.07278707275611795
32-33	0.06984311977624226
34-35	0.06962665264536903
36-37	0.08014695520580721
38-39	0.07529809147424724
40-41	0.07107698242221958
42-43	0.07444304630729803
44-45	0.08482264523266861
46-47	0.07488680392558814
48-49	0.07995213478802131
50-51	0.076899948242709
52-53	0.07595831622341052
54-55	0.07732205914791178
56-57	0.07107698242221958
58-59	0.07807969410596802
60-61	0.07503833091719937
62-63	0.06721304413613272
64-65	0.07005958690711547
66-67	0.06968076942808735
68-69	0.0681546761554312
70-71	0.06456132178293586
72-73	0.06210441984752488
74-75	0.06697493029217218
76-77	0.07146662325779135
78-79	0.06824126300778048
80-81	0.07501668420411206
82-83	0.07671595118146678
84-85	0.07804722403633704
86-87	0.0743456360984051
88-89	0.07610984321502179
90-91	0.07482186378632616
92-93	0.066693523022037
94-95	0.06624976540374693
96-97	0.06643376246498915
98-99	0.07465951343817126
100	0.08258221042813085
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4619639.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.37202992339776
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.47440253808055	46.16914918402965
2	14.089730633333414	17.012512788164383
3	4.421099827034308	8.007323131561318
4	1.854236047909961	4.47775976677852
5	0.9653251357288003	2.913931899001357
6	0.5529838839757028	2.0030855754322707
7	0.3322059914756476	1.4039165040669874
8	0.23165039791117736	1.118816380356848
9	0.1623987529241542	0.8823908133953565
>10	0.8282463008661735	8.952500866840643
>50	0.05526052657050353	2.288223489080125
>100	0.03034523404594909	3.368480934164656
>500	0.0015054015849480973	0.5898163849581686
>1k	5.73485702231048E-4	0.4639630851345831
>5k	0.0	0.0
>10k+	3.5842856389352004E-5	0.34812919703514195
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16088	0.3482523201488255	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	2.1646713087321324E-5	0.0	0.0	4.329342617464265E-5	0.0
10-11	2.1646713087321324E-5	0.0	0.0	4.329342617464265E-5	0.0
12-13	2.1646713087321324E-5	0.0	0.0	4.329342617464265E-5	0.0
14-15	2.1646713087321324E-5	0.0	0.0	4.329342617464265E-5	0.0
16-17	2.1646713087321324E-5	0.0	0.0	4.329342617464265E-5	0.0
18-19	2.1646713087321324E-5	0.0	0.0	4.329342617464265E-5	0.0
20-21	2.1646713087321324E-5	2.1646713087321324E-5	0.0	8.65868523492853E-5	0.0
22-23	2.1646713087321324E-5	2.1646713087321324E-5	0.0	2.4893720050419524E-4	0.0
24-25	2.1646713087321324E-5	2.1646713087321324E-5	0.0	5.195211140957118E-4	0.0
26-27	2.1646713087321324E-5	2.1646713087321324E-5	0.0	8.875152365801744E-4	2.1646713087321324E-5
28-29	2.1646713087321324E-5	2.1646713087321324E-5	0.0	0.002370315083061685	4.329342617464265E-5
30-31	2.1646713087321324E-5	2.1646713087321324E-5	0.0	0.00571473225505283	4.329342617464265E-5
32-33	2.1646713087321324E-5	2.1646713087321324E-5	0.0	0.012132982685443602	4.329342617464265E-5
34-35	2.1646713087321324E-5	2.1646713087321324E-5	0.0	0.02251258161081418	4.329342617464265E-5
36-37	2.1646713087321324E-5	2.1646713087321324E-5	0.0	0.03919137404459526	4.329342617464265E-5
38-39	2.1646713087321324E-5	2.1646713087321324E-5	0.0	0.06354392626783176	4.329342617464265E-5
40-41	2.1646713087321324E-5	2.1646713087321324E-5	0.0	0.08863246673603717	4.329342617464265E-5
42-43	2.1646713087321324E-5	2.1646713087321324E-5	0.0	0.11392665097857213	4.329342617464265E-5
44-45	2.1646713087321324E-5	2.1646713087321324E-5	0.0	0.14517368132012048	4.329342617464265E-5
46-47	2.1646713087321324E-5	2.1646713087321324E-5	0.0	0.18463563927830723	4.329342617464265E-5
48-49	2.1646713087321324E-5	2.1646713087321324E-5	0.0	0.22840529314087096	4.329342617464265E-5
50-51	2.1646713087321324E-5	2.1646713087321324E-5	0.0	0.2760821787156962	4.329342617464265E-5
52-53	2.1646713087321324E-5	2.1646713087321324E-5	0.0	0.3213129857116541	4.329342617464265E-5
54-55	2.1646713087321324E-5	2.1646713087321324E-5	0.0	0.37197711769252967	4.329342617464265E-5
56-57	2.1646713087321324E-5	2.1646713087321324E-5	0.0	0.43421141781857847	5.411678271830331E-5
58-59	2.1646713087321324E-5	2.1646713087321324E-5	0.0	0.5047905258397897	6.494013926196398E-5
60-61	2.1646713087321324E-5	2.1646713087321324E-5	0.0	0.5826970462410591	6.494013926196398E-5
62-63	2.1646713087321324E-5	2.1646713087321324E-5	0.0	0.661988956279917	6.494013926196398E-5
64-65	2.1646713087321324E-5	2.1646713087321324E-5	0.0	0.7474285328355743	6.494013926196398E-5
66-67	2.1646713087321324E-5	2.1646713087321324E-5	0.0	0.8456396701127513	6.494013926196398E-5
68-69	2.1646713087321324E-5	3.247006963098199E-5	0.0	0.9601291356315937	6.494013926196398E-5
70-71	2.1646713087321324E-5	4.329342617464265E-5	0.0	1.0917086811328764	6.494013926196398E-5
72-73	2.1646713087321324E-5	4.329342617464265E-5	0.0	1.2302692916048201	6.494013926196398E-5
74-75	2.1646713087321324E-5	4.329342617464265E-5	0.0	1.3678990934140094	6.494013926196398E-5
76-77	2.1646713087321324E-5	4.329342617464265E-5	0.0	1.522857089049599	6.494013926196398E-5
78-79	2.1646713087321324E-5	4.329342617464265E-5	0.0	1.7031200922842673	6.494013926196398E-5
80-81	2.1646713087321324E-5	4.329342617464265E-5	0.0	1.90645849167002	6.494013926196398E-5
82-83	2.1646713087321324E-5	4.329342617464265E-5	0.0	2.1251552339912276	6.494013926196398E-5
84-85	2.1646713087321324E-5	4.329342617464265E-5	0.0	2.348148848860268	6.494013926196398E-5
86-87	2.1646713087321324E-5	4.329342617464265E-5	0.0	2.583318739840927	6.494013926196398E-5
88	2.1646713087321324E-5	4.329342617464265E-5	0.0	2.777814456930509	6.494013926196398E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6050	0.0	47.670967	1
GTATCAA	10805	0.0	38.187717	1
TCAACGC	12545	0.0	31.722927	4
ATCAACG	12625	0.0	31.45046	3
CAACGCA	13495	0.0	29.523643	5
TATCAAC	14335	0.0	28.454224	2
AACGCAG	14380	0.0	27.844542	6
GTACATG	24020	0.0	27.206842	1
CATGGGT	10690	0.0	26.710655	4
TACATGG	23945	0.0	26.563616	2
TACCTGG	3675	0.0	25.987474	2
ACATGGG	24500	0.0	25.174019	3
CGCAGAG	16115	0.0	24.146267	8
GTACCTG	4140	0.0	24.092642	1
ACGCAGA	16475	0.0	23.76152	7
GTGGTAT	3200	0.0	21.024948	1
GCAGAGT	17865	0.0	20.93965	9
ATGGGTA	4875	0.0	20.648977	5
ATGGGTG	5925	0.0	20.562258	5
ACCTGGG	4575	0.0	20.154911	3
>>END_MODULE