FastQCFastQC Report
Wed 25 May 2016
SRR2431391_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431391_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4619639
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268590.5814090668123635No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221000.4783923592298013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146310.3167130591805983No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46860.10143649752718774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120500.064.583781
GTATCAA239600.045.3746721
ATCAACG312300.034.5669173
TCAACGC315400.034.227174
TATCAAC320650.033.8148652
CAACGCA326550.033.0584875
GTGGTAT38450.032.531031
AACGCAG339250.031.918326
TGGTATC40850.029.581532
CGCAGAG381700.028.1715838
ACGCAGA383000.028.1250467
TATAACG4600.026.5763342
TACCTGG35000.026.3310152
GTACATG237800.026.1415981
GCAGAGT411000.026.0031559
TACATGG241100.025.2162722
CATGGGT108150.024.954344
ACATGGG240700.024.612473
ATAACGC5350.024.6073593
GAGTACT271300.023.13510712-13
Produced by FastQC (version 0.11.5)